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ES2648798T3 - Materiales de captura de polinucleótidos y métodos de utilización de los mismos - Google Patents

Materiales de captura de polinucleótidos y métodos de utilización de los mismos Download PDF

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ES2648798T3
ES2648798T3 ES08826342.1T ES08826342T ES2648798T3 ES 2648798 T3 ES2648798 T3 ES 2648798T3 ES 08826342 T ES08826342 T ES 08826342T ES 2648798 T3 ES2648798 T3 ES 2648798T3
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Sundaresh N. Brahmasandra
Elizabeth Boutt
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Abstract

Un método para aislar polinucleótidos desde una muestra que contiene células, comprendiendo dicho método: contacto de la muestra con una solución de lisis y una pluralidad de partículas de unión revestidas en moléculas de PAMAM (Generación 0) covalentemente unidas con grupos carboxílicos en la superficie de las partículas de unión, donde cada moléculas de PAMAM (Generación 0) incluye exactamente cinco grupos amina disponibles para protonación, de manera que los polinucleótidos se liberan desde las células y se unen a los polinucleótidos desde dichas células con las partículas revestidas con PAMAM (Generación 0), en virtud de lo cual se crean partículas de unión unidas con polinucleótidos y una solución que contiene materia celular residual; compactación de las partículas de unión unidas con polinucleótidos; retirada de la solución que contiene la materia celular residual; lavado de las partículas de unión y liberación de los polinucleótidos desde las partículas de unión.

Description

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puntas de pipeta 103, 123 y 133 y por lo tanto representa una reutilización de una de dichas puntas. Aunque es deseable el uso de perlas magnéticas, también se utilizan perlas no magnéticas en el presente documento y se separan, p.ej., por centrifugación en lugar de mediante el uso de un imán.
5 En ciertas realizaciones, la relación entre el volumen de la muestra original introducida en el tubo de procesamiento y el volumen de líquido en el que se libera el ARN o ADN es al menos aproximadamente 10 (p.ej., al menos aproximadamente 50, al menos aproximadamente 100, al menos aproximadamente 250, al menos aproximadamente 500, al menos aproximadamente 1,000). En algunas realizaciones, se puede retener ARN o ADN de una muestra que tiene un volumen de aproximadamente 2 ml dentro del tubo de procesamiento y liberarse tras la unión y el lavado en aproximadamente 4 microlitros o menos (p.ej., aproximadamente 3 microlitros o menos, aproximadamente 2 microlitros o menos, aproximadamente 1 microlitros o menos) del líquido.
En algunas realizaciones, la muestra tiene un volumen mayor que el volumen concentrado de las partículas de unión que tienen unido en ellas ARN o ADN en un factor de al menos aproximadamente 10.
15 En otras realizaciones, la muestra tiene un volumen de 100 μl – 1 ml y las partículas compactadas ocupan un volumen efectivo de menos de 2 microlitros.
El líquido en el que se libera ARN o ADN incluye normalmente al menos aproximadamente 50 % (p.ej., al menos aproximadamente 75 %, al menos aproximadamente 85 %, al menos aproximadamente 90 % o al menos aproximadamente 95 %) del ARN o ADN, respectivamente, presente en la muestra 109. Así por ejemplo, se pueden liberar ∼8 -10 μg de ADN desde 1 ml de un cultivo de toda la noche y se pueden extraer ∼2 -4 μg de ADN desde una torunda bucal. La concentración de ARN o ADN presente en el líquido de liberación puede ser más alta que la correspondiente concentración en la muestra original ya que el volumen del líquido de liberación es normalmente
25 menos que el volumen de la muestra líquida original. Por ejemplo, la concentración de ADN en el líquido de liberación puede ser al menos aproximadamente 10 veces mayor (p.ej., al menos aproximadamente 25 veces mayor, al menos aproximadamente 100 veces mayor) que la concentración del ADN en la muestra 109. La concentración de inhibidores presentes en el líquido en el que se libera ARN o ADN es generalmente menos que la concentración de los inhibidores de la muestra fluídica original en una cantidad suficiente como para aumentar la eficiencia de amplificación del ARN o ADN con respecto a la que se podría obtener de una muestra sin purificar.
En general, aunque los procesos y materiales descritos en el presente documento tienen capacidad para actuar perfectamente – normalmente solamente con una adaptación de rutina – en una amplia gama de tamaños de muestra y volúmenes de reactivos, para la mayoría de las aplicaciones prácticas (considerando el tamaño de la 35 mayoría de las muestras biológicas sometidas a análisis de diagnóstico), el volumen de las partículas compactadas que tienen ARN y/o ADN unidos a ellas que resulta (antes de liberación) está en el intervalo de 2 – 3 μl y es independiente del volumen de la muestra, hasta aproximadamente 2 ml de la muestra. Normalmente, la cantidad de las micropartículas requeridas se determina por la cantidad de ARN y/o ADN en la muestra. Se ha observado que, dada la eficiencia de la unión a las partículas, es suficiente 0,5 mg de partículas para la mayoría de las aplicaciones manuales y para la mayoría que implican un pipeteado automático, independientemente del tamaño de la muestra. Por lo tanto, por ejemplo, para muestras que tienen volúmenes de 0,5 microlitros a 3 microlitros, el volumen de las partículas compactadas es de 2-3 μl. Por ejemplo, para Chlamydia, el tamaño de la muestra es normalmente 1 ml, y es suficiente 0,5 mg de partículas. Para otras aplicaciones, se puede extraer también ADN de una muestra de 2 ml con 0,5 mg de partículas o, en algunos casos, se pueden usar perlas de 1 mg. Para muestras más pequeñas, como
45 por ejemplo las que tienen un volumen de 50 ml, suele ser todavía lo normal utilizar partículas de 0,5 mg.
Para agitar la solución en varias etapas durante el proceso manual, se puede pipetear la solución arriba y abajo varias veces, por ejemplo 10 veces, 15 veces o 20 veces. Dicho procedimiento es aceptable durante la etapa de liberación, así como las etapas de lavado. La agitación con vórtice también funciona para estas etapas. Sin embargo, para los procesos automáticos, es imposible tolerar ninguna etapa de mezclado, el número de operaciones de mezclado se mantiene en el mínimo ya que posiblemente fuera la causa de que parte del PAMAM(0) se saliera e inhibiera la PCR en dirección 3’.
El proceso descrito en el presente documento representa una limpieza enormemente eficaz de una muestra en la
55 preparación de PCR y proporciona la capacidad de detectar tan solo 25 copias de ARN o ADN desde 1 mililitro de muestra clínica. EL ARN o ADN está presente en un alto nivel de concentración ya que el volumen de elución puede ser de tan solo 3 microlitros. Asimismo hay un reducido líquido de muestra residual y/o volumen de lavado en las microesferas concentradas, minimizando la dilución de la muestra o el tampón de lavado, además de minimizar la inhibición de la muestra residual.
El intervalo de tiempo entre la introducción de la muestra que contiene el polinucleótido en tubo de procesamiento 101 y la liberación del ARN o ADN en el líquido de liberación está comprendido normalmente entre 10 y 30 minutos, y es normalmente aproximadamente 15 -20 minutos o puede ser 15 minutos o menos (p.ej., aproximadamente 10 minutos o menos, aproximadamente 5 minutos o menos). Estos períodos de tiempo incluyen el período de lisis (que
65 duplica el período de unión a muestra) y son enormemente rápidos. Para liberar ARN y ADN, por separado, de una sola muestra, tan solo es necesario añadir un procedimiento de liberación adicional como en 140 en la FIG. 1.
8
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EL pKa de un grupo amino se define por su base conjugada, del siguiente modo : una amina protonada, R-NH3+ está en equilibrio disociativo:
R-NH3+ ↔ H+ + R-NH2 5 y su pKa se da por –log10 Ka, donde Ka = [H+] [R-NH2] / [R-NH3+].
Dado que un átomo de nitrógeno es trivalente y debido a las condiciones de dendrimerización, cada molécula de PAMAM(0) tiene una mezcla de grupos amina primaria, terciaria. Por lo tanto, las moléculas PAMAM (0) presentan múltiples valores pKa a lo largo de un intervalo de valores aproximadamente en consonancia con el intervalo de pKa cubierto por las aminas alifáticas primarias y terciarias, cuyos pKa se encuentran en el intervalo de 10-11 normalmente, tal como lo prueba por ejemplo, en la Tabla 12.2 de Organic Chemistry, 2ª Ed., Allinger, et al., Eds., Worth Publishers, Inc. (1976). Sin embargo, de acuerdo con la información facilitada por el fabricante de PAMAM (0), Dendritech of Midland, Michigan, es probable que de hecho PAMAM tengan pKa en el intervalo de 5,5 (para las
15 aminas terciarias en el interior de la molécula) – 9,5 (para las aminas primarias en la superficie de las moléculas de PAMAM). El artículo Tomalia, et al., Angew. Chem. Int. Ed. Engl., 29, 138-175 (1990), en la página 163, columna derecha de la publicación, se hace referencia estos datos.
PAMAM(0) es eficaz como agente de unión para ADN en los procesos descritos en el presente documento al menos en parte porque los grupos amina de PAMAM(0) tienen un pKa comprendido entre 5 -9. Por tanto, a un pH bajo, normalmente está cargado positivamente – y puede llevar incluso cargas positivas múltiples por molécula que tienen lugar a partir de las protonaciones de los grupos amina a pH más bajos que su pKa – y por tanto es capaz de unirse fuertemente a los polinucleótidos, como ADN y ARN, que normalmente comprenden polianiones (de carga negativa predominantemente) en solución.
25 Durante el uso de la molécula PAMAM (0) en los procesos descritos en el presente documento, el pH del tampón de unión (normalmente TRIS) utilizado para lisar células en el mismo momento en el que se une el ADN liberado con las partículas, es aproximadamente 7-8. A este pH, todas las aminas (6 grupos posibles por cada molécula PEI, distribuidas por Sigma) permanecen protonadas (con carga positiva) y, por tanto, atraen intensamente las moléculas de ADN cargadas negativamente para su unión hacia las perlas.
Las moléculas PAMAM (0) también son ventajosas porque son resistentes, p.ej. son inmunes, a la degradación de enzimas líticas, enzimas de proteasa (p.ej. mezclas de endo-y exo-proteasas, como pronasa que escinden los enlaces péptido), sustancias químicas duras, como detergentes, y calor hasta 95 ºC, y como tales son capaces de
35 unirse a ARN y ADN durante el proceso de lisis también. Por lo tanto, la lisis de células y la unión de ARN y/o ADN se pueden combinar en una sola etapa (sincrónica), en virtud de lo cual se ahorra tiempo y al menos una etapa de procesamiento. La unión fuerte de moléculas de ARN y/o ADN a PAMAM (0) permite un rápido lavado de las perlas de afinidad revestidas en PAMAM (0) para retirar los inhibidores de PCR utilizando una solución de lavado. La retirada de ARN y/o ADN desde las perlas de afinidad se lleva a efecto elevando la temperatura en presencia de un reactivo de retirada patentado. Dado que la cantidad de las perlas utilizada es muy reducida, (< 1 μl), el ARN y/o el ADN se pueden liberar en un volumen final de tan solo 3 microlitros. El ARN y/o ADN liberado se neutraliza a un volumen final de 5 a 50 microlitros utilizando un reactivo de neutralización y queda listo entonces para PCR en dirección 3’.
45 Normalmente, la cantidad de la muestra introducida es aproximadamente 500 microlitros o menos (p.ej., aproximadamente 250 microlitros o menos, aproximadamente 100 microlitros o menos, aproximadamente 50 microlitros o menos, aproximadamente 25 microlitros o menos, aproximadamente 10 microlitros o menos). En algunas realizaciones, la cantidad de la muestra es aproximadamente 2 microlitros o menos (p.ej., aproximadamente
0.5 microlitros o menos).
PAMAM(0) proporciona una recuperación de ARN y ADN excelente, sobre la base en parte de su alta capacidad de unión y su alta eficiencia de liberación. En general, la relación entre la masa de partículas y la masa de ARN o ADN retenida por las partículas no es más de aproximadamente 25 o más (p.ej., no más de aproximadamente 20, no más de aproximadamente 10). Por ejemplo, En algunas realizaciones, aproximadamente 1 gramo de partículas retiene
55 aproximadamente 100 miligramos de ARN o ADN; cuando se utiliza en cantidades más reducidas, se pueden obtener relaciones similares (p.ej., una capacidad de unión de ∼100 mg de ARN o ADN /mg perlas).
Otro aparato para captura de ADN
En otros ejemplos, se puede configurar el soporta sólido como un elemento de retención (p.ej., un elemento poroso como pueda ser una columna, un filtro, una membrana porosa, un filtro microporoso o una matriz de ge que tiene múltiples aperturas, como poros y/o canales, a través de los cuales pasa el ARN y/o ADN), a través del cual debe pasar el material de muestra (que contiene ARN y/o ADN). Dicho elemento de retención puede estar formado de múltiples partículas modificadas en su superficie restringidas en una geometría adecuada. En algunos ejemplos, el 65 elemento de retención comprende una o más membranas de filtro disponibles, por ejemplo, de Osmonics, que se forman de polímeros que pueden estar modificados en su superficie también y utilizarse para retener ARN y/o ADN.
13
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tampón y plasma. Se utilizaron 500 copias de ARN de EV13 por 1 ml tanto en las muestras de tampón como las de plasma de acuerdo con un proceso, tal como se describe con mayor detalle en el presente documento.
La FIG. 6 muestra la extracción de ADN, utilizando perlas de PAMAM (0). Se extrajeron 2,5 pg ADN con adiciones 5 en un tampón M4, o un tampón de lisis tal como se describe en el presente documento utilizando el proceso para extraer ARN de un tampón de recogida M4 tal como se describe con mayor detalle en el presente documento..
La FIG. 7 muestra las curvas de PCR para la extracción de ARN desde una muestra de plasma de 500 μl que contiene 200 copias de ARN EV13, utilizando tratamiento con 7U ADNasa.
10 LA FIG. 8 muestra ejemplos de la sensibilidad del proceso. El análisis Probit revela una LOD de 50 copias /200 ml LCR.
Ejemplo 4: Ejemplo de protocolo para la extracción de ARN desde M4, torunda seca en 1X tampón TCEP, 15 muestras THB
Proceso previo de preparación de muestra (solamente requieren filtración las muestras de torunda)
Etapa
Acción
1
Pipetear 500 μl de la muestra en un tubo (tubo con tapón a presión 1,7 ml DOT) que contiene 500 μl de tampón TCEP
2
Pipetear de arriba a abajo 2x, y a continuación pipetear la cantidad entera en la jeringuilla de 3 ml
3
Insertar el émbolo en la jeringuilla y filtrar el contenido en un tubo limpio (tubo con tapón a presión 1,7 ml DOT), aplicando presión hasta que se expulsa el líquido y empieza a salir la espuma del filtro (evitar que la espuma llegue a la muestra).
Extracción de ARN y preparación PCR
Etapa
Acción
1
Pipetear muestra (500 μl de muestra, más 500 μl de tampón TCEP) en un tubo con tapón a presión 1,7 ml DOT que contiene 30 μl de perlas magnéticas de ARN. Cerrar e invertir el tubo de reacción 5 veces o hasta que se dispersan las perlas (o se disuelven en el caso de perlas liofilizadas).
2
Inmediatamente colocar las muestras en un baño de agua a 60 °C, e incubar durante 10 min (10,5 minutos, máximo).
3
Retirar muestras del baño de agua y secar la parte exterior del tubo con un paño absorbente.
4
Colocar los tubos sobre una rejilla magnética y permitir que tenga lugar la separación durante 1 minuto (1,5 minutos max).
5
Utilizando una pipeta nueva para cada muestra, aspirar cuidadosamente 1 ml de sobrenadante de cada muestra (sin perturbar las perlas), utilizando una pipeta de 1 ml. Descartar el sobrenadante. Asegurarse de retirar cualquier líquido que quede en el tapón del tubo.
6
Tras la retirada del sobrenadantes inicial de todas las muestras, retirar cualquier líquido que quede utilizando una punta pipeta de 1 ml nueva para cada muestra.
7
Colocar tubos en una rejilla para tubos no magnética y añadir 100 μl de tampón de lavado (0,1 mM Tris, pH 7,5) a cada tubo utilizando una punta de pipeta de 200 ul. Pipetear de arriba a abajo 10 veces, o hasta resuspender todas las perlas magnéticas y que no quede pegada ninguna perla en la punta de la pipeta.
8
Colocar los tubos sobre la rejilla magnética durante 30 segundos, dejando que se separen las perlas.
9
Aspirar cuidadosamente el sobrenadante de todas las muestras utilizando una punta de pipeta de 200 ul. Descartar el sobrenadante. Utilizando una punta nueva de 20 ul para cada muestra, aspirar cualquier líquido que quede en la muestra (es decir, líquido que se ha "sedimentado" tras la primera etapa de aspiración) y descartar el líquido.
10
Colocar los tubos en una rejilla de tubos no magnética y añadir 10 μl de Tampón de liberación (20 mM Bis-Tris Propano o 20 mM Tris pH 9). Agitar con vórtice durante 10 segundos o hasta resuspender las perlas.
Etapa
Acción
11
Colocar las muestras en un bloque de calor a 85 °C durante 3 minutos (3,5 minutos max.).
12
Retirar las muestras del bloque de calor y colocar encima de la rejilla magnética durante 30 segundos (1 minutos max.).
13
Manteniendo los tubos sobre la rejilla magnética, retirar todo el líquido, evitando cuidadosamente las perlas magnéticas a un lado del tubo y colocar en un tubo 0,65 ml DOT.
14
Mezclar la muestra pipeteando de arriba a abajo una vez. La muestra queda lista ahora para PCR.
15
Preparar la mezcla de PCR utilizando un kit Quantitect con adiciones de cebadores 0,6 uM y extra platinum taq.
16
Añadir 8 ul de la mezcla en instrumentos capilares Rotorgene o LC, añadir 2 ul de ARN.
16
Etapa
Acción
17
Poner en marcha el programa RT PCR del siguiente modo: 50 grados durante 20 min (etapa a temperatura ambiente), 95 grados durante 5 min (desnaturalización), ciclar a 95-2 s, 58-50 s (50 ciclos)
Ejemplo 5: Ejemplo de protocolo para la extracción de ARN desde muestras de plasma
Extracción de ARN y preparación PCR
Etapa
Acción
1
Pipetear la muestra (500 μl de muestra, más 500 μl de tampón TCEP, 70 μl 10 % SDS) en un tubo con tapón a presión 1.7 ml DOT que contiene 30 ml de perlas magnéticas de ARN. Cerrar e invertir el tubo de reacción 5 veces o hasta que se dispersan las perlas (o se disuelven en el caso de perlas liofilizadas).
2
Inmediatamente colocar las muestras en un baño de agua a 60 °C, e incubar durante 10 min (10,5 minutos, máximo).
3
Retirar las muestras del baño de agua y secar la parte exterior del tubo con un paño absorbente.
4
Colocar los tubos sobre una rejilla magnética y permitir que tenga lugar la separación durante 1 minuto (1,5 minutos max).
5
Utilizando una pipeta nueva para cada muestra, aspirar cuidadosamente 1 ml de sobrenadante de cada muestra (sin perturbar las perlas), utilizando una pipeta 1 ml. Descartar el sobrenadante. Asegurarse de retirar cualquier líquido que quede en el tapón del tubo.
6
Tras la retirada del sobrenadantes inicial de todas las muestras, retirar cualquier líquido que quede utilizando una punta pipeta de 1 ml nueva para cada muestra.
7
Añadir 250 ul de tampón ADNasa con 1 aglomerado de ADNasa o 5 unidades de ADNasa líquida. Resuspender las perlas por agitación con vórtice o pipeteado.
8
Se incuba a 37 durante 10 min.
9
Colocar los tubos sobre una rejilla magnética y permitir la separación para proceder durante 1 minuto (1,5 minuto max).
10
Utilizando una pipeta nueva para cada muestra, aspirar cuidadosamente 1 ml de sobrenadante de cada muestra (sin perturbar las perlas), utilizando una pipeta de 1 ml. Descartar el sobrenadante. Asegurarse de retirar cualquier líquido que quede en el tapón del tubo.
11
Colocar tubos en una rejilla para tubos no magnética y añadir 100 μl de tampón de lavado (0,1 mM Tris, pH 7,5) a cada tubo utilizando una punta de pipeta de 200 ul. Pipetear de arriba a abajo 10 veces, o hasta resuspender que todas las perlas magnéticas y que no queda pegada ninguna perla en la punta de la pipeta.
12
Colocar los tubos sobre la rejilla magnética durante 30 segundos, dejando que se separen las perlas.
13
Aspirar cuidadosamente el sobrenadante de todas las muestras utilizando una punta de pipeta de 200 ul. Descartar el sobrenadante. Utilizando una punta nueva de 20 ul para cada muestra, aspirar cualquier líquido que quede en la muestra (es decir, líquido que se ha "sedimentado" tras la primera etapa de aspiración) y descartar el líquido.
14
Colocar los tubos en una rejilla de tubos no magnética y añadir 10 μl de Tampón de liberación (20 mM Bis-Tris Propano o 20 mM Tris pH 9). Agitar con vórtice durante 10 segundos o hasta resuspender las perlas.
15
Colocar las muestras en un bloque de calor a 85 °C durante 3 minutos (3,5 minutos max.).
16
Retirar las muestras del bloque de calor y colocar encima de la rejilla magnética durante 30 segundos (1 minutos max.).
17
Manteniendo los tubos sobre la rejilla magnética, retirar todo el líquido, evitando cuidadosamente las perlas magnéticas a un lado del tubo y colocar en un tubo 0,65 ml DOT.
18
Mezclar la muestra pipeteando de arriba a abajo una vez. La muestra queda lista ahora para PCR.
19
Preparar la mezcla de PCR utilizando un kit Quantitect con la adición de cebadores 0,6 uM y extra platinum taq.
20
Añadir 8 ul de la mezcla en instrumentos capilares Rotorgene o LC, añadir 2 ul de ARN.
21
Poner en marcha el programa RT PCR del siguiente modo: 50 grados durante 20 min (etapa a temperatura ambiente), 95 grados durante 5 min (desnaturalización), ciclar a 95-2 s, 58-50 s (50 ciclos)
5
Ejemplo 6: Proceso de ensamblaje para 2X Tampón TCEP para extracciones de ARN
El procedimiento en este ejemplo proporciona un método apropiado para preparar 50 ml de 2 x tampón TCEP (20 mM Tris HCl pH 7,0, 2 % Tx-100, 10 mM TCEP) utilizado en extracciones de ARN, tal como se describe en el 10 presente documento con mayor detalle. A continuación, se ofrece una lista de los reactivos utilizados en el proceso.
• 1 M Tris-HCl pH 7,0
17
imagen13
imagen14
Etapa
Acción
3
Etiquetar tubos cónicos 1 -50 ml con número de lote, fecha e iniciales
4
Pipetear la cantidad apropiada de microesferas en un tubo cónico de 50 ml
5
Colocar los tubos cónicos en una rejilla magnética y dejar asentar hasta que las perlas son capturadas completamente por el imán. Retirar sobrenadante cuidadosamente
Lavado tampón MES
Durante 30 minutos
6
Añadir Tampón SN-B a cada tubo y agitar con vórtice para mezclar Colocar los tubos cónicos en una rejilla magnética y dejar reposar hasta que se capturan las perlas completamente con el imán. Retirar el sobrenadante cuidadosamente. repetir el lavado 2 veces más 10 minutos
Preparar sulfo-NHS
7
Pesar una pequeña cantidad de sulfo-NHS balanza para papel y multiplicar el peso (en mg) por 20 para calcular los μl de agua ultra pura que se deben añadir para obtener una solución de 50 mg/ml. Se necesitan 1,5 ml para 1 reacción (75 mg). Añadir a un tubo de 1,7 ml y mezclar bien. Pesar (mg) X 20 = μl agua ultra pura necesaria. Añadir agua ultra pura y agitar con vórtice hasta que se resuspende. Se deberá preparar la solución NHS inmediatamente antes de su uso y descartar al cabo de 15 min
Activación (Preparar EDAC en campana)
8
Añadir los reactivos en el siguiente orden a cada tubo cónico Durante 30 minutos
(i) ddH2O
5000 ml
(ii) Tampón SN-C
1000 ml
(iii) 50 mg/ml sulfo-NHS
1500 ml
Sonicar utilizando un desmembrador ultrasonido a una potencia de 12 durante un período apropiado asegurándose de que la sonda está sumergida en todas las ocasiones. Limpiar la sonda con H2Od y secar con un trapo antes de la sonicación
10 segundos
9
Inmediatamente preparar 5 mg/ml EDAC en la campana. Se deberá preparar la solución de EDAC inmediatamente antes de su uso, descartar al cabo de 15 min. Pesar una pequeña cantidad de EDAC en papel de peso y multiplicar el peso (en mg) por 200 para calcular los μl de agua ultra pura necesarios para obtener una solución de 5 mg/ml. Se necesitan 2480 ml en total (12,4 mg). Preparar en tubos cónicos de 50 ml. Pesar (mg) X 200 = (ml) agua ultra pura que se ha de añadir Agitar con vórtice a fondo después de añadir H2O ultra pura
10
Añadir los siguientes: (i) 10% Triton X-100 (ii) 5 mg/ml EDAC (añadir solución de EDAC cuidadosamente; gota a gota mientras se agita con vórtice la solución a una velocidad muy baja). 10 ml 2480 ml
Activación (Preparar EDAC en campana)
(iii) 50 mg/ml sulfo-NHS
1500 ml
Mezclar bien con vórtice
11
Registrar el número de veces que EDAC abrió el frasco. Descartar tras 5 usos.
12
Asegurar los tubos para el agitador orbital con cinta de etiquetado e incubar a temperatura ambiente en el ajuste 6 (o en un ajuste en el que las microesferas se mezclan bien) 30 minutos.
13
Tras la incubación, centrifugar durante 5 min a una velocidad máxima y a continuación colocar en el imán. Retirar el sobrenadante cuidadosamente pero completamente 5 minutos
Tampón de lavado MES
Durante 30 minutos
14
Añadir tampón SN-B a cada tubo y agitar con vórtice para mezclar Colocar tubos cónicos en una rejilla magnética y dejar en reposo hasta que las perlas son capturadas totalmente por el imán. Retirar el sobrenadante cuidadosamente. Repetir el lavado 2 veces más. 10 minutos
Acoplamiento
15
Preparar reacción de acoplamiento Durante 10 minutos
(i) Añadir Tampón H
6 ml
20
imagen15

Claims (1)

  1. imagen1
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Families Citing this family (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
EP2402089A1 (en) * 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
EP1745153B1 (en) 2004-05-03 2015-09-30 Handylab, Inc. Processing polynucleotide-containing samples
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8088616B2 (en) 2006-03-24 2012-01-03 Handylab, Inc. Heater unit for microfluidic diagnostic system
EP2001990B1 (en) 2006-03-24 2016-06-29 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8765076B2 (en) 2006-11-14 2014-07-01 Handylab, Inc. Microfluidic valve and method of making same
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
US9618139B2 (en) * 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
ES2648798T3 (es) 2007-07-13 2018-01-08 Handylab, Inc. Materiales de captura de polinucleótidos y métodos de utilización de los mismos
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
TWI429475B (zh) * 2009-10-30 2014-03-11 Rbc Bioscience Corp 一體式反應匣
EP2388324A1 (de) * 2010-05-20 2011-11-23 Siemens Healthcare Diagnostics Inc. Kontrollmaterial für Nukleinsäureteste
WO2011153244A1 (en) * 2010-06-01 2011-12-08 Streck, Inc. Sample processing method and device
US9475709B2 (en) 2010-08-25 2016-10-25 Lockheed Martin Corporation Perforated graphene deionization or desalination
GB201100152D0 (en) 2011-01-06 2011-02-23 Epistem Ltd Genedrive RFID
GB2502761B (en) 2011-03-09 2015-09-30 Becton Dickinson Infusion Therapy Systems Inc Method and internal control composition for determining the efficacy of enzymatic cell lysis
CN106190806B (zh) 2011-04-15 2018-11-06 贝克顿·迪金森公司 扫描实时微流体热循环仪和用于同步的热循环和扫描光学检测的方法
US8809069B2 (en) 2011-05-20 2014-08-19 Perkinelmer Health Sciences, Inc. Lab members and liquid handling systems and methods including same
US9720406B2 (en) * 2011-08-15 2017-08-01 Endress+Hauser Conducta Gmbh+Co. Kg Measuring system
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
CN106996984B (zh) * 2011-09-30 2018-11-27 贝克顿·迪金森公司 组合试剂条
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
CA2863637C (en) 2012-02-03 2021-10-26 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
US11485968B2 (en) 2012-02-13 2022-11-01 Neumodx Molecular, Inc. Microfluidic cartridge for processing and detecting nucleic acids
US9637775B2 (en) 2012-02-13 2017-05-02 Neumodx Molecular, Inc. System and method for processing biological samples
US11648561B2 (en) 2012-02-13 2023-05-16 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US9339812B2 (en) 2012-02-13 2016-05-17 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US9604213B2 (en) 2012-02-13 2017-03-28 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
CN104620112B (zh) 2012-04-13 2017-11-28 贝克顿·迪金森公司 使用先前检验的残余材料反射检验样本
US10376845B2 (en) 2016-04-14 2019-08-13 Lockheed Martin Corporation Membranes with tunable selectivity
US10653824B2 (en) 2012-05-25 2020-05-19 Lockheed Martin Corporation Two-dimensional materials and uses thereof
US9610546B2 (en) 2014-03-12 2017-04-04 Lockheed Martin Corporation Separation membranes formed from perforated graphene and methods for use thereof
US9834809B2 (en) 2014-02-28 2017-12-05 Lockheed Martin Corporation Syringe for obtaining nano-sized materials for selective assays and related methods of use
US9744617B2 (en) 2014-01-31 2017-08-29 Lockheed Martin Corporation Methods for perforating multi-layer graphene through ion bombardment
EP2912174B1 (en) 2012-10-25 2019-06-19 Neumodx Molecular, Inc. Method and materials for isolation of nucleic acid materials
EP2972353B1 (en) * 2013-03-11 2023-02-22 Meso Scale Technologies, LLC Improved methods for conducting multiplexed assays
US9592475B2 (en) 2013-03-12 2017-03-14 Lockheed Martin Corporation Method for forming perforated graphene with uniform aperture size
AU2013202778A1 (en) 2013-03-14 2014-10-02 Gen-Probe Incorporated Systems, methods, and apparatuses for performing automated reagent-based assays
GB201304797D0 (en) 2013-03-15 2013-05-01 Diagnostics For The Real World Ltd Apparatus and method for automated sample preparation and adaptor for use in the apparatus
JP6615744B2 (ja) 2013-03-15 2019-12-04 ベクトン・ディキンソン・アンド・カンパニー ナイセリア・ゴノレアの検出
US9572918B2 (en) 2013-06-21 2017-02-21 Lockheed Martin Corporation Graphene-based filter for isolating a substance from blood
AU2015210875A1 (en) 2014-01-31 2016-09-15 Lockheed Martin Corporation Processes for forming composite structures with a two-dimensional material using a porous, non-sacrificial supporting layer
KR20160142820A (ko) 2014-01-31 2016-12-13 록히드 마틴 코포레이션 브로드 이온 필드를 사용한 2차원 물질 천공
EP3116625A4 (en) 2014-03-12 2017-12-20 Lockheed Martin Corporation Separation membranes formed from perforated graphene
USD814653S1 (en) 2014-08-07 2018-04-03 Becton, Dickinson And Company Sample tube holder and components thereof
MX2017002738A (es) 2014-09-02 2017-08-02 Lockheed Corp Membranas de hemodialisis y hemofiltracion basadas en un material de membrana bidimensional y metodos que emplean las mismas.
KR20230118714A (ko) 2015-04-24 2023-08-11 벡톤 디킨슨 앤드 컴퍼니 외음질 칸디다증, 질편모충증 및 세균성 질염의 복합검출 방법
WO2017023376A1 (en) 2015-08-05 2017-02-09 Lockheed Martin Corporation Perforatable sheets of graphene-based material
MX2018001559A (es) 2015-08-06 2018-09-27 Lockheed Corp Modificacion de nanoparticula y perforacion de grafeno.
US10351893B2 (en) 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells
JP1565699S (es) * 2016-01-12 2016-12-19
WO2017132129A1 (en) * 2016-01-26 2017-08-03 Nutech Ventures Expedited pcr with stirring
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads
JP2019519756A (ja) 2016-04-14 2019-07-11 ロッキード・マーチン・コーポレーション 欠陥形成または欠陥修復をその場で監視して制御する方法
JP2019511451A (ja) 2016-04-14 2019-04-25 ロッキード・マーチン・コーポレーション 浮遊法を用いてグラフェンシートを大判転写用に処理する方法
EP3442786A4 (en) 2016-04-14 2020-03-18 Lockheed Martin Corporation TWO-DIMENSIONAL MEMBRANE STRUCTURES HAVING FLOW PASSAGES
WO2017180134A1 (en) 2016-04-14 2017-10-19 Lockheed Martin Corporation Methods for in vivo and in vitro use of graphene and other two-dimensional materials
WO2017180141A1 (en) 2016-04-14 2017-10-19 Lockheed Martin Corporation Selective interfacial mitigation of graphene defects
USD814652S1 (en) * 2016-10-14 2018-04-03 Spartan Bioscience In. Cartridge
FR3058734B1 (fr) * 2016-11-15 2020-10-30 Biomerieux Sa Systeme et procede d’extraction pour extraire des micro-organismes contenus dans un echantillon
US11077444B2 (en) * 2017-05-23 2021-08-03 Roche Molecular Systems, Inc. Packaging for a molecular diagnostic cartridge
USD836210S1 (en) * 2017-07-14 2018-12-18 Nipro Corporation Cell preservation container
US10816565B2 (en) * 2017-12-08 2020-10-27 Michael Doran Test tube removal device and system
JP1606642S (es) * 2017-12-14 2018-06-11
CN111601621A (zh) * 2018-01-18 2020-08-28 豪夫迈·罗氏有限公司 用二胺交联剂的超交联
USD907797S1 (en) * 2019-06-19 2021-01-12 Life Technologies Corporation Reagent strip
WO2022060548A1 (en) * 2020-09-18 2022-03-24 Microgem International Plc Method and system for sample collection, storage, preparation and detection
CN116507742A (zh) 2020-11-05 2023-07-28 贝克顿迪金森公司 霍乱弧菌的多重检测和分型
AU2021373166A1 (en) 2020-11-05 2023-05-11 Becton, Dickinson And Company Multiplex detection of bacterial respiratory pathogens
JP2023547535A (ja) 2020-11-05 2023-11-10 ベクトン・ディキンソン・アンド・カンパニー ビブリオ・パラヘモリティクスの迅速な同定及び分類
USD998172S1 (en) * 2021-03-12 2023-09-05 Ultima Genomics, Inc. Sample rack
WO2023084370A1 (en) * 2021-11-12 2023-05-19 3M Innovative Properties Company Solid support comprising ligands suitable for polynucleic acid processing, articles and methods
EP4430181A1 (en) * 2021-11-12 2024-09-18 Solventum Intellectual Properties Company Method of processing polynucleic acids
KR102505472B1 (ko) * 2022-09-05 2023-03-06 주식회사 제놀루션 핵산 포획용 자성 입자 및 이를 이용한 핵산 포획 또는 선별 방법
US20240345114A1 (en) * 2023-04-13 2024-10-17 Beckman Coulter, Inc. Sample preparation
GB2629182A (en) * 2023-04-20 2024-10-23 Ttp Plc A modular biological processing cartridge and container

Family Cites Families (1046)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1434314A (en) 1921-08-04 1922-10-31 Raich Anthony Lunch pail
US1616419A (en) 1925-04-03 1927-02-01 Everlasting Valve Co Automatic shut-off device for gas in case of fire
US1733401A (en) 1928-03-29 1929-10-29 Christman Matthias Journal box
NL109202C (es) 1959-11-20
GB1095429A (es) 1965-05-17
US3528449A (en) 1968-02-27 1970-09-15 Trw Inc Fluid flow control apparatus
US3813316A (en) 1972-06-07 1974-05-28 Gen Electric Microorganisms having multiple compatible degradative energy-generating plasmids and preparation thereof
US4038192A (en) 1973-12-03 1977-07-26 International Biomedical Laboratories, Inc. Device for exchange between fluids suitable for treatment of blood
US3985649A (en) 1974-11-25 1976-10-12 Eddelman Roy T Ferromagnetic separation process and material
JPS5255578A (en) 1975-10-31 1977-05-07 Hitachi Ltd Analyzing apparatus
US4018652A (en) 1976-01-09 1977-04-19 Mcdonnell Douglas Corporation Process and apparatus for ascertaining the concentration of microorganism in a water specimen
US4018089A (en) 1976-05-05 1977-04-19 Beckman Instruments, Inc. Fluid sampling apparatus
USD249706S (en) 1976-12-17 1978-09-26 Eastman Kodak Company Sample cup tray for chemical analysis of biological fluids
USD252157S (en) 1977-04-14 1979-06-19 Warner-Lambert Company Diagnostic device for measuring biochemical characteristics of microorganisms and the like
USD252341S (en) 1977-05-12 1979-07-10 Ryder International Corporation Testing tray
JPS5416896A (en) 1977-06-21 1979-02-07 Asahi Medical Co Blood dialyser
US4139005A (en) 1977-09-01 1979-02-13 Dickey Gilbert C Safety release pipe cap
USD254687S (en) 1979-01-25 1980-04-08 Mcdonnell Douglas Corporation Biochemical card for use with an automated microbial identification machine
USD261173S (en) 1979-02-05 1981-10-06 American Optical Corporation Bilirubinometer
USD261033S (en) 1979-02-05 1981-09-29 American Optical Corporation Bilirubin concentration analyzer
US4301412A (en) 1979-10-29 1981-11-17 United States Surgical Corporation Liquid conductivity measuring system and sample cards therefor
JPS5766361A (en) 1980-10-09 1982-04-22 Olympus Optical Co Ltd Plate-shaped apparatus for judging cohesion of particle
US4472357A (en) 1981-11-18 1984-09-18 Medical Laboratory Automation, Inc. Blood bank cuvette cassette and label therefor
US4457329A (en) 1981-12-04 1984-07-03 Air Products And Chemicals, Inc. Safety pressure regulator
JPS58212921A (ja) 1982-06-04 1983-12-10 Aron Kasei Co Ltd プラスチツクス中空成形物の成形方法
USD279817S (en) 1982-07-19 1985-07-23 Daryl Laboratories, Inc. Immunoassay test slide
US4504582A (en) 1982-07-20 1985-03-12 Genex Corporation Vermiculite as a carrier support for immobilized biological materials
US4439526A (en) 1982-07-26 1984-03-27 Eastman Kodak Company Clustered ingress apertures for capillary transport devices and method of use
ATE34618T1 (de) 1982-09-02 1988-06-15 Hettich Andreas Fa Zentrifugationskammern zur zytodiagnostischen praeparation von epithelzellen und deren verwendung.
US4647432A (en) 1982-11-30 1987-03-03 Japan Tectron Instruments Corporation Tokuyama Soda Kabushiki Kaisha Automatic analysis apparatus
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
USD282208S (en) 1983-02-07 1986-01-14 Data Packaging Corporation Pipetter tip cartridge
US4698302A (en) 1983-05-12 1987-10-06 Advanced Magnetics, Inc. Enzymatic reactions using magnetic particles
US4522786A (en) 1983-08-10 1985-06-11 E. I. Du Pont De Nemours And Company Multilayered test device for detecting analytes in liquid test samples
US4673657A (en) 1983-08-26 1987-06-16 The Regents Of The University Of California Multiple assay card and system
CA1220168A (en) 1983-09-09 1987-04-07 Henry J. Rahn Magnetic separator for solid phase immunoassays
US4599315A (en) 1983-09-13 1986-07-08 University Of California Regents Microdroplet test apparatus
USD292735S (en) 1983-11-02 1987-11-10 A/S Nunc Tube for the immunological adsorption analysis
US4724207A (en) 1984-02-02 1988-02-09 Cuno Incorporated Modified siliceous chromatographic supports
US4654127A (en) 1984-04-11 1987-03-31 Sentech Medical Corporation Self-calibrating single-use sensing device for clinical chemistry and method of use
JP2502961B2 (ja) 1984-04-26 1996-05-29 日本碍子株式会社 電気化学的装置の製造方法
USD288478S (en) 1984-06-21 1987-02-24 Sentech Medical Corporation Clinical chemistry analyzer
WO1986005410A1 (en) 1985-03-13 1986-09-25 Baxter Travenol Laboratories, Inc. Platelet collection system
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4612959A (en) 1985-05-07 1986-09-23 Mobil Oil Corporation Valveless shut-off and transfer device
US4720374A (en) 1985-07-22 1988-01-19 E. I. Du Pont De Nemours And Company Container having a sonication compartment
US4963498A (en) 1985-08-05 1990-10-16 Biotrack Capillary flow device
US4795698A (en) 1985-10-04 1989-01-03 Immunicon Corporation Magnetic-polymer particles
US4678752A (en) 1985-11-18 1987-07-07 Becton, Dickinson And Company Automatic random access analyzer
US4871779A (en) * 1985-12-23 1989-10-03 The Dow Chemical Company Ion exchange/chelation resins containing dense star polymers having ion exchange or chelate capabilities
JPS62119460U (es) 1986-01-22 1987-07-29
JPH0720010B2 (ja) 1986-03-05 1995-03-06 沖電気工業株式会社 円偏波変形ビ−ムアンテナ
DE3614955C1 (de) 1986-05-02 1987-08-06 Schulz Peter Proben-Verteiler-System
US4978622A (en) 1986-06-23 1990-12-18 Regents Of The University Of California Cytophaga-derived immunopotentiator
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5763262A (en) 1986-09-18 1998-06-09 Quidel Corporation Immunodiagnostic device
USD302294S (en) 1986-10-03 1989-07-18 Biotrack, Inc. Reagent cartridge for blood analysis
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US4978502A (en) 1987-01-05 1990-12-18 Dole Associates, Inc. Immunoassay or diagnostic device and method of manufacture
US4946562A (en) 1987-01-29 1990-08-07 Medtest Systems, Inc. Apparatus and methods for sensing fluid components
US5004583A (en) 1987-01-29 1991-04-02 Medtest Systems, Inc. Universal sensor cartridge for use with a universal analyzer for sensing components in a multicomponent fluid
US4997772A (en) 1987-09-18 1991-03-05 Eastman Kodak Company Water-insoluble particle and immunoreactive reagent, analytical elements and methods of use
ES2054797T3 (es) 1987-03-02 1994-08-16 Gen Probe Inc Soportes policationicos para purificar, separar e hibridar acidos nucleicos.
US5599667A (en) 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
US4855110A (en) 1987-05-06 1989-08-08 Abbott Laboratories Sample ring for clinical analyzer network
US5001417A (en) 1987-06-01 1991-03-19 Abbott Laboratories Apparatus for measuring electrolytes utilizing optical signals related to the concentration of the electrolytes
US5192507A (en) 1987-06-05 1993-03-09 Arthur D. Little, Inc. Receptor-based biosensors
JPH0354470Y2 (es) 1987-07-15 1991-12-02
JPH01503808A (ja) 1987-07-16 1989-12-21 イー・アイ・デユポン・ド・ネモアース・アンド・コンパニー（インコーポレイテツド） 固定化された凝集剤を用いるアフイニテイ分離
US4827944A (en) 1987-07-22 1989-05-09 Becton, Dickinson And Company Body fluid sample collection tube composite
GB8720470D0 (en) 1987-08-29 1987-10-07 Emi Plc Thorn Sensor arrangements
US4921809A (en) 1987-09-29 1990-05-01 Findley Adhesives, Inc. Polymer coated solid matrices and use in immunoassays
USD312692S (en) 1987-10-09 1990-12-04 Bradley Marshall C Pipette holder
US4914710A (en) 1987-11-20 1990-04-03 Storage Technology Corporation MICR document smear test machine
US4902624A (en) 1987-11-23 1990-02-20 Eastman Kodak Company Temperature cycling cuvette
USD310413S (en) 1987-12-17 1990-09-04 Miles Inc. Sample processor
US4895650A (en) 1988-02-25 1990-01-23 Gen-Probe Incorporated Magnetic separation rack for diagnostic assays
JPH01219669A (ja) 1988-02-29 1989-09-01 Shimadzu Corp 液体試料容器の類別検出方法
US5503803A (en) 1988-03-28 1996-04-02 Conception Technologies, Inc. Miniaturized biological assembly
DE3811713A1 (de) 1988-04-08 1989-10-19 Bosch Gmbh Robert Planare polarographische sonde zur bestimmung des (lambda)-wertes von gasgemischen
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US4919892A (en) 1988-06-07 1990-04-24 Plumb Arnold D Apparatus for detecting oil and other lighter-than-water contaminants in an effluent stream
US5096669A (en) 1988-09-15 1992-03-17 I-Stat Corporation Disposable sensing device for real time fluid analysis
US5060823A (en) 1988-09-15 1991-10-29 Brandeis University Sterile transfer system
US6147198A (en) 1988-09-15 2000-11-14 New York University Methods and compositions for the manipulation and characterization of individual nucleic acid molecules
DE8813340U1 (de) 1988-10-24 1988-12-08 Laboratorium Prof. Dr. Rudolf Berthold, 7547 Wildbad Probenrack für Probengefäße
JPH0814337B2 (ja) 1988-11-11 1996-02-14 株式会社日立製作所 流体自体の相変化を利用した流路の開閉制御弁及び開閉制御方法
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US4919829A (en) 1988-12-30 1990-04-24 The United States Of America As Represented By The Secretary Of Commerce Aluminum hydroxides as solid lubricants
US5229297A (en) 1989-02-03 1993-07-20 Eastman Kodak Company Containment cuvette for PCR and method of use
US4948561A (en) 1989-02-09 1990-08-14 Eastman Kodak Company Multiple level filter device and kit containing same
US5053199A (en) 1989-02-21 1991-10-01 Boehringer Mannheim Corporation Electronically readable information carrier
US5416000A (en) 1989-03-16 1995-05-16 Chemtrak, Inc. Analyte immunoassay in self-contained apparatus
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US6610256B2 (en) 1989-04-05 2003-08-26 Wisconsin Alumni Research Foundation Image processing and analysis of individual nucleic acid molecules
FI86229C (fi) 1989-04-10 1992-07-27 Niilo Kaartinen Foerfarande foer formning av ett uppvaermbart och nedkylbart element vid ett system behandlande smao vaetskemaengder samt ett medelst foerfarandet framstaellt element.
US5334499A (en) 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US4949742A (en) 1989-04-26 1990-08-21 Spectra-Physics, Inc. Temperature operated gas valve
US5135872A (en) 1989-04-28 1992-08-04 Sangstat Medical Corporation Matrix controlled method of delayed fluid delivery for assays
US5061336A (en) 1989-05-01 1991-10-29 Soane Technologies, Inc. Gel casting method and apparatus
US5071531A (en) 1989-05-01 1991-12-10 Soane Technologies, Inc. Casting of gradient gels
AU109440S (en) 1989-05-03 1990-10-31 Bayer Diagnostic G M B H Diagnostic working station evaluation device
US5089233A (en) 1989-06-12 1992-02-18 Eastman Kodak Company Processing apparatus for a chemical reaction pack
US5173269A (en) 1989-06-15 1992-12-22 At&T Bell Laboratories Apparatus for reducing the reactivity of articles destined for disposal
USD325638S (en) 1989-07-10 1992-04-21 Hach Company Microtester or the like
USD328794S (en) 1989-07-19 1992-08-18 Pb Diagnostic Systems, Inc. Diagnostic instrument or similar article
AU635314B2 (en) 1989-09-08 1993-03-18 Terumo Kabushiki Kaisha Measuring apparatus
EP0420560B1 (en) 1989-09-29 1993-11-18 Glory Kogyo Kabushiki Kaisha Leaf paper bundling apparatus
US5275787A (en) 1989-10-04 1994-01-04 Canon Kabushiki Kaisha Apparatus for separating or measuring particles to be examined in a sample fluid
LU87601A1 (fr) 1989-10-05 1990-02-07 Ceodeux Sa Robinet pour bouteille de gaz
US5231015A (en) 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
USD324426S (en) 1989-10-20 1992-03-03 Pacific Biotech, Inc. Reaction unit for use in analyzing biological fluids
US4967950A (en) 1989-10-31 1990-11-06 International Business Machines Corporation Soldering method
US5252743A (en) 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
US5091328A (en) 1989-11-21 1992-02-25 National Semiconductor Corporation Method of late programming MOS devices
JP2731613B2 (ja) 1989-12-12 1998-03-25 株式会社クラレ 酵素免疫測定用カートリツジ、それを用いた測定方法及び測定装置
US5279936A (en) 1989-12-22 1994-01-18 Syntex (U.S.A.) Inc. Method of separation employing magnetic particles and second medium
USD328135S (en) 1990-01-12 1992-07-21 Pacific Biotech, Inc. Reaction unit for use in analyzing biological fluids
US5935401A (en) 1996-09-18 1999-08-10 Aclara Biosciences Surface modified electrophoretic chambers
US5770029A (en) 1996-07-30 1998-06-23 Soane Biosciences Integrated electrophoretic microdevices
US5858188A (en) 1990-02-28 1999-01-12 Aclara Biosciences, Inc. Acrylic microchannels and their use in electrophoretic applications
US6054034A (en) 1990-02-28 2000-04-25 Aclara Biosciences, Inc. Acrylic microchannels and their use in electrophoretic applications
US5126022A (en) 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
US5750015A (en) 1990-02-28 1998-05-12 Soane Biosciences Method and device for moving molecules by the application of a plurality of electrical fields
GB9007791D0 (en) 1990-04-06 1990-06-06 Foss Richard C High voltage boosted wordline supply charge pump and regulator for dram
WO1991016675A1 (en) 1990-04-06 1991-10-31 Applied Biosystems, Inc. Automated molecular biology laboratory
WO1991017446A1 (en) 1990-05-01 1991-11-14 Autogen Instruments, Inc. Integral biomolecule preparation device
US5667976A (en) 1990-05-11 1997-09-16 Becton Dickinson And Company Solid supports for nucleic acid hybridization assays
US5935522A (en) 1990-06-04 1999-08-10 University Of Utah Research Foundation On-line DNA analysis system with rapid thermal cycling
US5147777A (en) 1990-06-18 1992-09-15 Eastman Kodak Company Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use
US5155166A (en) 1990-06-18 1992-10-13 Eastman Kodak Company Use of 1-(1-pyrrolidinylcarbonyl)pyridinium salts to attach compounds to carboxylated particles and a kit containing same
DE4023194A1 (de) 1990-07-20 1992-01-23 Kodak Ag Vorrichtung mit mehreren einreihig angeordneten aufnahmen fuer mit fluessigkeit gefuellte behaelter
US5208163A (en) 1990-08-06 1993-05-04 Miles Inc. Self-metering fluid analysis device
US5147606A (en) 1990-08-06 1992-09-15 Miles Inc. Self-metering fluid analysis device
JPH0734375Y2 (ja) 1990-09-11 1995-08-02 株式会社シノテスト 被検体物質の反応測定検出器具
WO1992005443A1 (en) 1990-09-15 1992-04-02 Medical Research Council Reagent separation
US5135627A (en) 1990-10-15 1992-08-04 Soane Technologies, Inc. Mosaic microcolumns, slabs, and separation media for electrophoresis and chromatography
US5652141A (en) 1990-10-26 1997-07-29 Oiagen Gmbh Device and process for isolating nucleic acids from cell suspension
US5141718A (en) 1990-10-30 1992-08-25 Millipore Corporation Test plate apparatus
EP0484278B1 (de) 1990-11-01 1995-04-12 Ciba-Geigy Ag Vorrichtung zur Aufbereitung oder Vorbereitung von flüssigen Proben für eine chemische Analyse
EP0488947B1 (de) 1990-11-26 1995-02-15 Ciba-Geigy Ag Detektorzelle
US6703236B2 (en) 1990-11-29 2004-03-09 Applera Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5273716A (en) 1991-01-14 1993-12-28 Electric Power Research Institute, Inc. pH optrode
FR2672301A1 (fr) 1991-02-01 1992-08-07 Larzul Daniel Procede et dispositif pour amplifier le nombre d'une sequence definie d'acide nucleique dans un echantillon biologique.
US5466574A (en) 1991-03-25 1995-11-14 Immunivest Corporation Apparatus and methods for magnetic separation featuring external magnetic means
US5384499A (en) 1991-04-25 1995-01-24 Altera Corporation High-density erasable programmable logic device architecture using multiplexer interconnections
US5327038A (en) 1991-05-09 1994-07-05 Rockwell International Corporation Walking expansion actuator
JPH05345900A (ja) 1991-07-08 1993-12-27 Fuji Oil Co Ltd 硬質油脂の製造法
DE4123348A1 (de) 1991-07-15 1993-01-21 Boehringer Mannheim Gmbh Elektrochemisches analysesystem
USD333522S (en) 1991-07-23 1993-02-23 P B Diagnostic Systems, Inc. Sample tube holder
IT1249433B (it) 1991-08-06 1995-02-23 Pompeo Moscetta Procedimento per il dosaggio di analiti in campioni liquidi e relativaapparecchiatura.
US7297313B1 (en) 1991-08-31 2007-11-20 The Regents Of The University Of California Microfabricated reactor, process for manufacturing the reactor, and method of amplification
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US5256376A (en) 1991-09-12 1993-10-26 Medical Laboratory Automation, Inc. Agglutination detection apparatus
US5582884A (en) 1991-10-04 1996-12-10 Alcan International Limited Peelable laminated structures and process for production thereof
CA2074671A1 (en) 1991-11-04 1993-05-05 Thomas Bormann Device and method for separating plasma from a biological fluid
USD347478S (en) 1991-11-05 1994-05-31 Hybaid Ltd. Laboratory instrument for handling bimolecular samples
US5849486A (en) 1993-11-01 1998-12-15 Nanogen, Inc. Methods for hybridization analysis utilizing electrically controlled hybridization
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
DE69231853T2 (de) 1991-11-07 2001-09-13 Nanotronics, Inc. Hybridisierung von mit chromophoren und fluorophoren konjugierten polynukleotiden zur erzeugung eines donor-zu-donor energietransfersystems
US5787032A (en) 1991-11-07 1998-07-28 Nanogen Deoxyribonucleic acid(DNA) optical storage using non-radiative energy transfer between a donor group, an acceptor group and a quencher group
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
USD340289S (en) * 1992-01-30 1993-10-12 Jan Gerber Diagnostic testing material
US5559432A (en) 1992-02-27 1996-09-24 Logue; Delmar L. Joystick generating a polar coordinates signal utilizing a rotating magnetic field within a hollow toroid core
US5217694A (en) 1992-03-25 1993-06-08 Gibler W Brian Holder for evacuated test tubes
US5646049A (en) 1992-03-27 1997-07-08 Abbott Laboratories Scheduling operation of an automated analytical system
EP1130117A3 (en) 1992-04-06 2003-12-03 Abbott Laboratories Method and device for detection of nucleic acid using total internal reflectance
US5223226A (en) 1992-04-14 1993-06-29 Millipore Corporation Insulated needle for forming an electrospray
US6235313B1 (en) 1992-04-24 2001-05-22 Brown University Research Foundation Bioadhesive microspheres and their use as drug delivery and imaging systems
US5744366A (en) 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
US5498392A (en) 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5726026A (en) 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5296375A (en) 1992-05-01 1994-03-22 Trustees Of The University Of Pennsylvania Mesoscale sperm handling devices
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5304487A (en) 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US6953676B1 (en) 1992-05-01 2005-10-11 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US5486335A (en) 1992-05-01 1996-01-23 Trustees Of The University Of Pennsylvania Analysis based on flow restriction
US5401465A (en) 1992-05-05 1995-03-28 Chiron Corporation Luminometer with reduced sample crosstalk
US5364591A (en) 1992-06-01 1994-11-15 Eastman Kodak Company Device for moving a target-bearing solid through a liquid for detection while being contained
US5414245A (en) 1992-08-03 1995-05-09 Hewlett-Packard Corporation Thermal-ink heater array using rectifying material
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
DE4231966A1 (de) 1992-09-24 1994-03-31 Bosch Gmbh Robert Planare polarograhische Sonde zur Bestimmung des Lambda-Wertes von Gasgemischen
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5422271A (en) 1992-11-20 1995-06-06 Eastman Kodak Company Nucleic acid material amplification and detection without washing
US5569364A (en) 1992-11-05 1996-10-29 Soane Biosciences, Inc. Separation media for electrophoresis
US5885432A (en) 1992-11-05 1999-03-23 Soane Biosciences Un-crosslinked polymeric media for electrophoresis
GB9223334D0 (en) 1992-11-06 1992-12-23 Hybaid Ltd Magnetic solid phase supports
US5500187A (en) 1992-12-08 1996-03-19 Westinghouse Electric Corporation Disposable optical agglutination assay device and method for use
US5302348A (en) 1992-12-10 1994-04-12 Itc Corporation Blood coagulation time test apparatus and method
US5311996A (en) 1993-01-05 1994-05-17 Duffy Thomas J Edge protector
DE4334834A1 (de) 1993-10-13 1995-04-20 Andrzej Dr Ing Grzegorzewski Biosensor zum Messen von Viskositäts- und/oder Dichteänderungen
FI932866A0 (fi) 1993-06-21 1993-06-21 Labsystems Oy Separeringsfoerfarande
JPH0664156U (ja) 1993-02-16 1994-09-09 株式会社ニッテク 血液容器の保持構造
USD351913S (en) 1993-02-25 1994-10-25 Diametrics Medical, Inc. Disposable electrochemical measurement cartridge for a portable medical analyzer
US5339486A (en) 1993-03-10 1994-08-23 Persic Jr William V Golf ball cleaner
DE4316023A1 (de) 1993-05-13 1994-11-17 Basf Ag Verfahren zur Herstellung von niedrigmolekularen Polymerisaten des 1-Vinylimidazols
US5565171A (en) 1993-05-28 1996-10-15 Governors Of The University Of Alberta Continuous biochemical reactor for analysis of sub-picomole quantities of complex organic molecules
JP3339650B2 (ja) 1993-07-02 2002-10-28 和光純薬工業株式会社 液体分注装置
EP0636413B1 (en) 1993-07-28 2001-11-14 PE Corporation (NY) Nucleic acid amplification reaction apparatus and method
USD356232S (en) 1993-08-20 1995-03-14 Flair Communications Agency, Inc. Dual vesselled beverage container
US5397709A (en) 1993-08-27 1995-03-14 Becton Dickinson And Company System for detecting bacterial growth in a plurality of culture vials
JP2948069B2 (ja) 1993-09-20 1999-09-13 株式会社日立製作所 化学分析装置
US5374395A (en) 1993-10-14 1994-12-20 Amoco Corporation Diagnostics instrument
US5645801A (en) 1993-10-21 1997-07-08 Abbott Laboratories Device and method for amplifying and detecting target nucleic acids
US5415839A (en) 1993-10-21 1995-05-16 Abbott Laboratories Apparatus and method for amplifying and detecting target nucleic acids
DE59410283D1 (de) 1993-11-11 2003-06-18 Aclara Biosciences Inc Vorrichtung und Verfahren zur elektrophoretischen Trennung von fluiden Substanzgemischen
DE4343089A1 (de) 1993-12-17 1995-06-29 Bosch Gmbh Robert Planares Sensorelement auf Festelektrolytbasis
CA2143365A1 (en) 1994-03-14 1995-09-15 Hugh V. Cottingham Nucleic acid amplification method and apparatus
DE4408361C2 (de) 1994-03-14 1996-02-01 Bosch Gmbh Robert Elektrochemischer Sensor zur Bestimmung der Sauerstoffkonzentration in Gasgemischen
US5725831A (en) 1994-03-14 1998-03-10 Becton Dickinson And Company Nucleic acid amplification apparatus
WO1995025815A1 (en) 1994-03-24 1995-09-28 Gamera Bioscience Corporation A dna meltometer and methods of use thereof
US5580523A (en) 1994-04-01 1996-12-03 Bard; Allen J. Integrated chemical synthesizers
US5475487A (en) 1994-04-20 1995-12-12 The Regents Of The University Of California Aqueous carrier waveguide in a flow cytometer
USD366116S (en) 1994-05-03 1996-01-09 Thomas Biskupski Electrical box for storing dental wax
DE69527585T2 (de) 1994-06-08 2003-04-03 Affymetrix, Inc. Verfahren und Vorrichtung zum Verpacken von Chips
DE4420732A1 (de) 1994-06-15 1995-12-21 Boehringer Mannheim Gmbh Vorrichtung zur Behandlung von Nukleinsäuren aus einer Probe
US5514343A (en) 1994-06-22 1996-05-07 Nunc, As Microtitration system
AU2907995A (en) 1994-06-23 1996-01-19 Dade International Inc. Method for the rapid isolation of nucleic acid
FR2722294B1 (fr) * 1994-07-07 1996-10-04 Lyon Ecole Centrale Procede d'analyse qualitative et/ou quantitative de substances biologiques presentes dans un milieu liquide conducteur et capteurs biochimiques d'affinite utilises pour la mise en oeuvre de ce procede
JPH10502692A (ja) * 1994-07-14 1998-03-10 トーンジェット コーポレイション プロプライエタリー リミテッド 固体インキジェットインキ
US5639428A (en) 1994-07-19 1997-06-17 Becton Dickinson And Company Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay
US6001229A (en) 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
CA2156226C (en) 1994-08-25 1999-02-23 Takayuki Taguchi Biological fluid analyzing device and method
US5627041A (en) 1994-09-02 1997-05-06 Biometric Imaging, Inc. Disposable cartridge for an assay of a biological sample
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
JP3403839B2 (ja) 1994-10-27 2003-05-06 プレシジョン・システム・サイエンス株式会社 カートリッジ容器
JP3652424B2 (ja) 1994-10-27 2005-05-25 日本政策投資銀行 自動分析装置及びその方法
US5721136A (en) 1994-11-09 1998-02-24 Mj Research, Inc. Sealing device for thermal cycling vessels
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
US5603351A (en) 1995-06-07 1997-02-18 David Sarnoff Research Center, Inc. Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device
ATE277450T1 (de) 1994-11-10 2004-10-15 Orchid Biosciences Inc Flüssigkeitsverteilungssystem
US5632876A (en) 1995-06-06 1997-05-27 David Sarnoff Research Center, Inc. Apparatus and methods for controlling fluid flow in microchannels
US5654141A (en) 1994-11-18 1997-08-05 Thomas Jefferson University Amplification based detection of bacterial infection
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5731212A (en) 1994-12-20 1998-03-24 International Technidyne Corporation Test apparatus and method for testing cuvette accommodated samples
US5846493A (en) 1995-02-14 1998-12-08 Promega Corporation System for analyzing a substance from a solution following filtering of the substance from the solution
US6884357B2 (en) 1995-02-21 2005-04-26 Iqbal Waheed Siddiqi Apparatus and method for processing magnetic particles
US5683659A (en) 1995-02-22 1997-11-04 Hovatter; Kenneth R. Integral assembly of microcentrifuge strip tubes and strip caps
US5579928A (en) * 1995-03-06 1996-12-03 Anukwuem; Chidi I. Test tube holder with lock down clamp
US6967088B1 (en) 1995-03-16 2005-11-22 Allergan, Inc. Soluble recombinant botulinum toxin proteins
US5578270A (en) 1995-03-24 1996-11-26 Becton Dickinson And Company System for nucleic acid based diagnostic assay
US5674394A (en) 1995-03-24 1997-10-07 Johnson & Johnson Medical, Inc. Single use system for preparation of autologous plasma
DE19512368A1 (de) 1995-04-01 1996-10-02 Boehringer Mannheim Gmbh System zur Freisetzung und Isolierung von Nukleinsäuren
US5700429A (en) 1995-04-19 1997-12-23 Roche Diagnostic Systems, Inc. Vessel holder for automated analyzer
USD382346S (en) 1995-04-19 1997-08-12 Roche Diagnostic Systems, Inc. Vessel holder
US5578818A (en) 1995-05-10 1996-11-26 Molecular Dynamics LED point scanning system
US5771902A (en) 1995-09-25 1998-06-30 Regents Of The University Of California Micromachined actuators/sensors for intratubular positioning/steering
DE19519015C1 (de) 1995-05-24 1996-09-05 Inst Physikalische Hochtech Ev Miniaturisierter Mehrkammer-Thermocycler
WO1996039547A2 (en) 1995-06-01 1996-12-12 The Regents Of The University Of California Multiple source deposition of shape-memory alloy thin films
JPH11506412A (ja) 1995-06-06 1999-06-08 デイヴィッド サーノフ リサーチ センター，インコーポレイテッド 超小型電気管路の製造方法
CA2222506C (en) 1995-06-06 2007-07-24 Quantic Biomedical Partners Device and method for concentrating plasma
US6228635B1 (en) 1995-06-07 2001-05-08 Aastrom Bioscience, Inc. Portable cell growth cassette for use in maintaining and growing biological cells
JP3554612B2 (ja) 1995-06-12 2004-08-18 マツダ株式会社 車両用ロールカーテン
US6524532B1 (en) 1995-06-20 2003-02-25 The Regents Of The University Of California Microfabricated sleeve devices for chemical reactions
US6521181B1 (en) 1995-06-20 2003-02-18 The Regents Of The University Of Calfornia Microfabricated electrochemiluminescence cell for chemical reaction detection
US5589136A (en) 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
US5968745A (en) * 1995-06-27 1999-10-19 The University Of North Carolina At Chapel Hill Polymer-electrodes for detecting nucleic acid hybridization and method of use thereof
US20020022261A1 (en) 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6168948B1 (en) 1995-06-29 2001-01-02 Affymetrix, Inc. Miniaturized genetic analysis systems and methods
US6158269A (en) 1995-07-13 2000-12-12 Bayer Corporation Method and apparatus for aspirating and dispensing sample fluids
US5872010A (en) 1995-07-21 1999-02-16 Northeastern University Microscale fluid handling system
JP3923968B2 (ja) 1995-07-31 2007-06-06 プレシジョン・システム・サイエンス株式会社 容器使用方法
EP2259070A3 (en) 1995-07-31 2011-03-30 Precision System Science Co., Ltd. Container
JP3927570B2 (ja) 1995-07-31 2007-06-13 プレシジョン・システム・サイエンス株式会社 容器
US5849208A (en) * 1995-09-07 1998-12-15 Microfab Technoologies, Inc. Making apparatus for conducting biochemical analyses
EP1291441A3 (en) 1995-09-12 2003-03-19 Becton, Dickinson and Company Device and method for DNA amplification and assay
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US6057149A (en) 1995-09-15 2000-05-02 The University Of Michigan Microscale devices and reactions in microscale devices
US6911183B1 (en) 1995-09-15 2005-06-28 The Regents Of The University Of Michigan Moving microdroplets
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
GB9519346D0 (en) 1995-09-22 1995-11-22 English Glass Company The Limi Dispensing systems
US5658515A (en) 1995-09-25 1997-08-19 Lee; Abraham P. Polymer micromold and fabrication process
US5628890A (en) 1995-09-27 1997-05-13 Medisense, Inc. Electrochemical sensor
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US6132580A (en) 1995-09-28 2000-10-17 The Regents Of The University Of California Miniature reaction chamber and devices incorporating same
DE69513658T2 (de) 1995-09-29 2000-05-31 Stmicroelectronics S.R.L., Agrate Brianza Spannungsregler für nichtflüchtige, elektrisch programmierbare Halbleiterspeicheranordnungen
US5651839A (en) 1995-10-26 1997-07-29 Queen's University At Kingston Process for engineering coherent twin and coincident site lattice grain boundaries in polycrystalline materials
US5705813A (en) 1995-11-01 1998-01-06 Hewlett-Packard Company Integrated planar liquid handling system for maldi-TOF MS
DE19540877C2 (de) 1995-11-02 1998-02-26 Byk Sangtec Diagnostica Modulare Reagenzienkartusche
WO1997016835A1 (en) 1995-11-03 1997-05-09 David Sarnoff Research Center Magnet
US20010055812A1 (en) 1995-12-05 2001-12-27 Alec Mian Devices and method for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics
JP2002503331A (ja) 1995-12-05 2002-01-29 ガメラ バイオサイエンス コーポレイション 機内に搭載された情報科学を備えた超微量液体素子工学システムにおいて液体移動を推進するために求心性加速を使用するための装置及び方法
US6068751A (en) 1995-12-18 2000-05-30 Neukermans; Armand P. Microfluidic valve and integrated microfluidic system
WO1997022825A1 (en) 1995-12-18 1997-06-26 Neukermans Armand P Microfluidic valve and integrated microfluidic system
US5872481A (en) * 1995-12-27 1999-02-16 Qualcomm Incorporated Efficient parallel-stage power amplifier
USD382647S (en) 1996-01-17 1997-08-19 Biomerieux Vitek, Inc. Biochemical test card
US5631337A (en) 1996-01-19 1997-05-20 Soane Bioscience Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis
US5883211A (en) 1996-01-19 1999-03-16 Aclara Biosciences, Inc. Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis
US5863502A (en) 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US5726944A (en) 1996-02-05 1998-03-10 Motorola, Inc. Voltage regulator for regulating an output voltage from a charge pump and method therefor
US5981735A (en) 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
US5736102A (en) 1996-02-21 1998-04-07 Biomerieux Vitek, Inc. Test sample positioning system
AU715627B2 (en) 1996-02-21 2000-02-03 Biomerieux Vitek, Inc. Automatic sample testing machine
USD378782S (en) 1996-03-01 1997-04-08 Johnson & Johnson Clinical Diagnostics, Inc. Processor for nucleic acid detection
US5849598A (en) 1996-03-15 1998-12-15 Washington University Method for transferring micro quantities of liquid samples to discrete locations
US5895762A (en) 1996-03-25 1999-04-20 Diasys Corporation Apparatus and method for handling fluid samples of body materials
US6114122A (en) 1996-03-26 2000-09-05 Affymetrix, Inc. Fluidics station with a mounting system and method of using
US5844238A (en) 1996-03-27 1998-12-01 David Sarnoff Research Center, Inc. Infrared imager using room temperature capacitance sensor
US7235406B1 (en) 1996-04-03 2007-06-26 Applera Corporation Nucleic acid analysis device
US7244622B2 (en) 1996-04-03 2007-07-17 Applera Corporation Device and method for multiple analyte detection
US5788814A (en) 1996-04-09 1998-08-04 David Sarnoff Research Center Chucks and methods for positioning multiple objects on a substrate
US6399023B1 (en) 1996-04-16 2002-06-04 Caliper Technologies Corp. Analytical system and method
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US5671303A (en) 1996-04-17 1997-09-23 Motorola, Inc. Molecular detection apparatus and method using optical waveguide detection
US5948363A (en) 1996-04-22 1999-09-07 Gaillard; Patrick Micro-well strip with print tabs
US6001307A (en) 1996-04-26 1999-12-14 Kyoto Daiichi Kagaku Co., Ltd. Device for analyzing a sample
US6221672B1 (en) 1996-04-30 2001-04-24 Medtronic, Inc. Method for determining platelet inhibitor response
US6054277A (en) 1996-05-08 2000-04-25 Regents Of The University Of Minnesota Integrated microchip genetic testing system
US6180950B1 (en) * 1996-05-14 2001-01-30 Don Olsen Micro heating apparatus for synthetic fibers
JPH09304385A (ja) 1996-05-14 1997-11-28 Shionogi & Co Ltd 磁性粒子分離装置及び物質の分離方法
JP3682302B2 (ja) 1996-05-20 2005-08-10 プレシジョン・システム・サイエンス株式会社 分注機による磁性体粒子の制御方法およびその装置
US5726404A (en) 1996-05-31 1998-03-10 University Of Washington Valveless liquid microswitch
US6083762A (en) 1996-05-31 2000-07-04 Packard Instruments Company Microvolume liquid handling system
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
US5914229A (en) 1996-06-14 1999-06-22 Sarnoff Corporation Method for amplifying a polynucleotide
US5912124A (en) 1996-06-14 1999-06-15 Sarnoff Corporation Padlock probe detection
US5863801A (en) 1996-06-14 1999-01-26 Sarnoff Corporation Automated nucleic acid isolation
WO1997048818A1 (en) 1996-06-17 1997-12-24 The Board Of Trustees Of The Leland Stanford Junior University Thermocycling apparatus and method
WO1998000705A1 (en) 1996-06-28 1998-01-08 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
US5779868A (en) 1996-06-28 1998-07-14 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
BR9710054A (pt) 1996-06-28 2000-01-11 Caliper Techn Corp Aparelhos para separar compostos de teste para um efeito sobre um sistema bioquìmico e para detectar ummefeito de um composto de teste sobre um sistema bioquìmico, processos de determinação de se uma amostra contém um composto capaz de afetar um sistema bioquìmico, de separação de uma pluralidade de compostos de teste para um efeito sobre um sistema bioquìmico e usos de um sistema microfluido e de um substrato de ensaio.
US5800690A (en) 1996-07-03 1998-09-01 Caliper Technologies Corporation Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces
US5699157A (en) 1996-07-16 1997-12-16 Caliper Technologies Corp. Fourier detection of species migrating in a microchannel
US6074827A (en) 1996-07-30 2000-06-13 Aclara Biosciences, Inc. Microfluidic method for nucleic acid purification and processing
US20020053399A1 (en) 1996-07-30 2002-05-09 Aclara Biosciences, Inc Methods for fabricating enclosed microchannel structures
US6558916B2 (en) 1996-08-02 2003-05-06 Axiom Biotechnologies, Inc. Cell flow apparatus and method for real-time measurements of patient cellular responses
US5804436A (en) 1996-08-02 1998-09-08 Axiom Biotechnologies, Inc. Apparatus and method for real-time measurement of cellular response
US6280967B1 (en) 1996-08-02 2001-08-28 Axiom Biotechnologies, Inc. Cell flow apparatus and method for real-time of cellular responses
US5872623A (en) * 1996-09-26 1999-02-16 Sarnoff Corporation Massively parallel detection
US5858187A (en) 1996-09-26 1999-01-12 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing electrodynamic focusing on a microchip
US6110343A (en) 1996-10-04 2000-08-29 Lockheed Martin Energy Research Corporation Material transport method and apparatus
WO1998016527A1 (en) 1996-10-15 1998-04-23 Fujisawa Pharmaceutical Co., Ltd. Benzoxepine derivatives which promote release of growth hormone
US6500390B1 (en) 1996-10-17 2002-12-31 David A. Boulton Method for sealing and venting a microplate assembly
US5874046A (en) 1996-10-30 1999-02-23 Raytheon Company Biological warfare agent sensor system employing ruthenium-terminated oligonucleotides complementary to target live agent DNA sequences
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
USD421653S (en) 1996-11-18 2000-03-14 Tekmar Company Housing for a laboratory instrument
US6447727B1 (en) 1996-11-19 2002-09-10 Caliper Technologies Corp. Microfluidic systems
DE19749011A1 (de) 1996-11-19 1998-05-20 Lang Volker Mikroventil
US6465257B1 (en) 1996-11-19 2002-10-15 Caliper Technologies Corp. Microfluidic systems
US6379929B1 (en) 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
US5772966A (en) 1997-01-24 1998-06-30 Maracas; George N. Assay dispensing apparatus
USD399959S (en) 1997-01-24 1998-10-20 Abbott Laboratories Housing for a device for measuring the concentration of an analyte in a sample of blood
USD414271S (en) 1997-02-03 1999-09-21 Eli Lilly And Company Reaction vessel for combining chemicals
US5972694A (en) 1997-02-11 1999-10-26 Mathus; Gregory Multi-well plate
DE19707226A1 (de) 1997-02-24 1998-08-27 Bodenseewerk Perkin Elmer Co Lichtabtastvorrichtung
US5882496A (en) 1997-02-27 1999-03-16 The Regents Of The University Of California Porous silicon structures with high surface area/specific pore size
US5911737A (en) 1997-02-28 1999-06-15 The Regents Of The University Of California Microfabricated therapeutic actuators
CA2574107C (en) 1997-02-28 2011-08-16 Cepheid Heat exchanging, optically interrogated chemical reaction assembly
US5958349A (en) 1997-02-28 1999-09-28 Cepheid Reaction vessel for heat-exchanging chemical processes
US8293064B2 (en) 1998-03-02 2012-10-23 Cepheid Method for fabricating a reaction vessel
US6565815B1 (en) 1997-02-28 2003-05-20 Cepheid Heat exchanging, optically interrogated chemical reaction assembly
US5861563A (en) 1997-03-20 1999-01-19 Bayer Corporation Automatic closed tube sampler
US5964997A (en) 1997-03-21 1999-10-12 Sarnoff Corporation Balanced asymmetric electronic pulse patterns for operating electrode-based pumps
US5747666A (en) 1997-03-26 1998-05-05 Willis; John P. Point-of-care analyzer module
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US5964995A (en) 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US5993750A (en) 1997-04-11 1999-11-30 Eastman Kodak Company Integrated ceramic micro-chemical plant
DE19717085C2 (de) 1997-04-23 1999-06-17 Bruker Daltonik Gmbh Verfahren und Geräte für extrem schnelle DNA-Vervielfachung durch Polymerase-Kettenreaktionen (PCR)
US5976336A (en) 1997-04-25 1999-11-02 Caliper Technologies Corp. Microfluidic devices incorporating improved channel geometries
KR100351531B1 (ko) 1997-04-25 2002-09-11 캘리퍼 테크놀로지스 코포레이션 기하형상이 개선된 채널을 채용하는 미소 유체 장치
US5997708A (en) 1997-04-30 1999-12-07 Hewlett-Packard Company Multilayer integrated assembly having specialized intermediary substrate
DK0979146T3 (da) 1997-05-02 2002-12-02 Gen Probe Inc Reaktionsbeholderapparat
WO1998050147A1 (en) 1997-05-09 1998-11-12 The Regents Of The University Of California Peltier-assisted microfabricated reaction chambers for thermal cycling
US5944717A (en) 1997-05-12 1999-08-31 The Regents Of The University Of California Micromachined electrical cauterizer
US6106685A (en) 1997-05-13 2000-08-22 Sarnoff Corporation Electrode combinations for pumping fluids
US5980719A (en) 1997-05-13 1999-11-09 Sarnoff Corporation Electrohydrodynamic receptor
AU7591998A (en) 1997-05-23 1998-12-11 Gamera Bioscience Corporation Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
JP3937107B2 (ja) 1997-05-23 2007-06-27 東拓工業株式会社 環状凹凸波形管用ベルマウス
US5869004A (en) 1997-06-09 1999-02-09 Caliper Technologies Corp. Methods and apparatus for in situ concentration and/or dilution of materials in microfluidic systems
EP1021566A4 (en) 1997-06-09 2002-06-19 Caliper Techn Corp DEVICE AND METHOD FOR CORRECTING VARIOUS SPEEDS IN MICROFLUIDIC SYSTEMS
ES2249818T3 (es) 1997-06-09 2006-04-01 F. Hoffmann-La Roche Ag Dispositivo desechable despues de su uso en un proceso.
US6190619B1 (en) 1997-06-11 2001-02-20 Argonaut Technologies, Inc. Systems and methods for parallel synthesis of compounds
US6425972B1 (en) 1997-06-18 2002-07-30 Calipher Technologies Corp. Methods of manufacturing microfabricated substrates
US5900130A (en) 1997-06-18 1999-05-04 Alcara Biosciences, Inc. Method for sample injection in microchannel device
US5882465A (en) 1997-06-18 1999-03-16 Caliper Technologies Corp. Method of manufacturing microfluidic devices
US5959291A (en) 1997-06-27 1999-09-28 Caliper Technologies Corporation Method and apparatus for measuring low power signals
JP3191150B2 (ja) 1997-06-30 2001-07-23 株式会社アステックコーポレーション 採血管ラック
US5928161A (en) 1997-07-03 1999-07-27 The Regents Of The University Of California Microbiopsy/precision cutting devices
US6001231A (en) 1997-07-15 1999-12-14 Caliper Technologies Corp. Methods and systems for monitoring and controlling fluid flow rates in microfluidic systems
US5985217A (en) 1997-07-17 1999-11-16 The Regents Of The University Of California Microfabricated instrument for tissue biopsy and analysis
US5932799A (en) 1997-07-21 1999-08-03 Ysi Incorporated Microfluidic analyzer module
US5827481A (en) 1997-07-31 1998-10-27 Hewlett-Packard Company Cartridge system for effecting sample acquisition and introduction
US5876675A (en) 1997-08-05 1999-03-02 Caliper Technologies Corp. Microfluidic devices and systems
US5919711A (en) 1997-08-07 1999-07-06 Careside, Inc. Analytical cartridge
US5916522A (en) 1997-08-07 1999-06-29 Careside, Inc. Electrochemical analytical cartridge
US6156199A (en) 1997-08-11 2000-12-05 Zuk, Jr.; Peter Centrifugal filtration apparatus
AU4437602A (en) 1997-08-13 2002-07-11 Cepheid Microstructures for the manipulation of fluid samples
JP2001515216A (ja) 1997-08-13 2001-09-18 シーフィード 流体試料を操作するための微小構造体
AU4437702A (en) 1997-08-13 2002-07-11 Cepheid Microstructures for the manipulation of fluid samples
US6368871B1 (en) 1997-08-13 2002-04-09 Cepheid Non-planar microstructures for manipulation of fluid samples
US5916776A (en) 1997-08-27 1999-06-29 Sarnoff Corporation Amplification method for a polynucleotide
US5989402A (en) 1997-08-29 1999-11-23 Caliper Technologies Corp. Controller/detector interfaces for microfluidic systems
CA2300203A1 (en) 1997-09-02 1999-03-11 Caliper Technologies Corporation Microfluidic system with electrofluidic and electrothermal controls
US5965410A (en) 1997-09-02 1999-10-12 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6043880A (en) 1997-09-15 2000-03-28 Becton Dickinson And Company Automated optical reader for nucleic acid assays
US6597450B1 (en) 1997-09-15 2003-07-22 Becton, Dickinson And Company Automated Optical Reader for Nucleic Acid Assays
US20020092767A1 (en) 1997-09-19 2002-07-18 Aclara Biosciences, Inc. Multiple array microfluidic device units
AU744879B2 (en) 1997-09-19 2002-03-07 Aclara Biosciences, Inc. Apparatus and method for transferring liquids
US6902703B2 (en) 1999-05-03 2005-06-07 Ljl Biosystems, Inc. Integrated sample-processing system
US5961925A (en) 1997-09-22 1999-10-05 Bristol-Myers Squibb Company Apparatus for synthesis of multiple organic compounds with pinch valve block
US5993611A (en) 1997-09-24 1999-11-30 Sarnoff Corporation Capacitive denaturation of nucleic acid
US6012902A (en) 1997-09-25 2000-01-11 Caliper Technologies Corp. Micropump
DE69839294T2 (de) 1997-09-29 2009-04-09 F. Hoffmann-La Roche Ag Gerät zur Abscheidung magnetischer Teilchen
AU9673198A (en) 1997-10-02 1999-04-27 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
AU1517999A (en) 1997-10-15 1999-05-03 Aclara Biosciences, Inc. Laminate microstructure device and method for making same
US5958694A (en) 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US6132684A (en) 1997-10-31 2000-10-17 Becton Dickinson And Company Sample tube holder
DE69841255D1 (de) 1997-11-14 2009-12-03 Gen Probe Inc Arbeitsgerät zur Analyse
AU1460299A (en) 1997-11-14 1999-06-07 California Institute Of Technology Cell lysis device
JP3477170B2 (ja) 1997-11-19 2003-12-10 イネックス ゲゼルシャフト ミット ベシュレンクテル ハフツング 超音波信号を用いて連続的に移動するプラスチックのストリップ又は管の中の欠陥を検出しかつ／又はそれらの壁厚を測定するための装置
US5992820A (en) 1997-11-19 1999-11-30 Sarnoff Corporation Flow control in microfluidics devices by controlled bubble formation
US6174675B1 (en) 1997-11-25 2001-01-16 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6123205A (en) 1997-11-26 2000-09-26 Bayer Corporation Sample tube rack
USD413677S (en) 1997-11-26 1999-09-07 Bayer Corporation Sample tube rack
US6197503B1 (en) 1997-11-26 2001-03-06 Ut-Battelle, Llc Integrated circuit biochip microsystem containing lens
US6258264B1 (en) 1998-04-10 2001-07-10 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
DE69805649T2 (de) 1997-12-06 2002-12-05 Dna Res Innovations Ltd Isolierung von nukleinsäuren
US6074725A (en) 1997-12-10 2000-06-13 Caliper Technologies Corp. Fabrication of microfluidic circuits by printing techniques
DE19755479A1 (de) 1997-12-13 1999-06-17 Wolfgang Prof Dr Ing Benecke Miniaturisiertes PCR-System zur Genanalyse
US5948227A (en) 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
JP4209589B2 (ja) 1997-12-24 2009-01-14 シーフィード 一体型流体操作カートリッジ
US6430512B1 (en) 1997-12-30 2002-08-06 Caliper Technologies Corp. Software for the display of chromatographic separation data
US6167910B1 (en) 1998-01-20 2001-01-02 Caliper Technologies Corp. Multi-layer microfluidic devices
JP3551293B2 (ja) 1998-02-02 2004-08-04 東洋紡績株式会社 核酸抽出装置
USD413391S (en) 1998-02-05 1999-08-31 Bayer Corporation Test tube sample rack
US6420143B1 (en) 1998-02-13 2002-07-16 Caliper Technologies Corp. Methods and systems for performing superheated reactions in microscale fluidic systems
US6537432B1 (en) 1998-02-24 2003-03-25 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US6756019B1 (en) 1998-02-24 2004-06-29 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US6100541A (en) 1998-02-24 2000-08-08 Caliper Technologies Corporation Microfluidic devices and systems incorporating integrated optical elements
US6861035B2 (en) 1998-02-24 2005-03-01 Aurora Discovery, Inc. Multi-well platforms, caddies, lids and combinations thereof
US6251343B1 (en) 1998-02-24 2001-06-26 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
USD417009S (en) 1998-03-02 1999-11-23 Bayer Corporation Sample tube rack
US6369893B1 (en) 1998-05-19 2002-04-09 Cepheid Multi-channel optical detection system
USD428497S (en) 1998-03-06 2000-07-18 Bayer Corporation Test tube sample rack
AU764319B2 (en) 1998-03-17 2003-08-14 Cepheid Chemical processing device
US6979424B2 (en) 1998-03-17 2005-12-27 Cepheid Integrated sample analysis device
ATE250455T1 (de) 1998-03-17 2003-10-15 Cepheid Vorrichtung zum analysieren einer probe
US7188001B2 (en) 1998-03-23 2007-03-06 Cepheid System and method for temperature control
AU1357102A (en) 1998-03-23 2002-03-14 Cepheid Multi-site reactor system with dynamic independent control of indvidual reaction sites
JPH11295323A (ja) 1998-04-13 1999-10-29 Matsushita Electric Ind Co Ltd 自動分注装置および分注方法
US6024920A (en) 1998-04-21 2000-02-15 Bio-Rad Laboratories, Inc. Microplate scanning read head
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
DE69942654D1 (de) 1998-05-01 2010-09-16 Gen Probe Inc Transportvorrichtung für automatische Analysevorrichtung
JPH11316226A (ja) 1998-05-06 1999-11-16 Olympus Optical Co Ltd 自動測定用カートリッジ及び自動測定法
US6123798A (en) 1998-05-06 2000-09-26 Caliper Technologies Corp. Methods of fabricating polymeric structures incorporating microscale fluidic elements
US6818437B1 (en) 1998-05-16 2004-11-16 Applera Corporation Instrument for monitoring polymerase chain reaction of DNA
CA2320296A1 (en) 1998-05-18 1999-11-25 University Of Washington Liquid analysis cartridge
EP0964555A1 (de) 1998-06-04 1999-12-15 Siemens Aktiengesellschaft Pegelregelung und adaptive Filterung in CAP-Empfängern
US6274089B1 (en) 1998-06-08 2001-08-14 Caliper Technologies Corp. Microfluidic devices, systems and methods for performing integrated reactions and separations
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
US6149882A (en) 1998-06-09 2000-11-21 Symyx Technologies, Inc. Parallel fixed bed reactor and fluid contacting apparatus
USD421130S (en) 1998-06-15 2000-02-22 Bayer Corporation Sample tube rack
US7799521B2 (en) 1998-06-24 2010-09-21 Chen & Chen, Llc Thermal cycling
US6780617B2 (en) 2000-12-29 2004-08-24 Chen & Chen, Llc Sample processing device and method
US6375901B1 (en) 1998-06-29 2002-04-23 Agilent Technologies, Inc. Chemico-mechanical microvalve and devices comprising the same
US6787111B2 (en) 1998-07-02 2004-09-07 Amersham Biosciences (Sv) Corp. Apparatus and method for filling and cleaning channels and inlet ports in microchips used for biological analysis
USD420747S (en) 1998-07-10 2000-02-15 Bayer Corporation Sample tube rack
DE19833087A1 (de) 1998-07-23 2000-01-27 Bosch Gmbh Robert Gassensor und Verfahren zu dessen Herstellung
DE19833293C1 (de) 1998-07-24 2000-01-20 Gunther Botsch Trennvorrichtung
US20020049694A1 (en) 1998-07-27 2002-04-25 J. Wallace Parce Distributed database for analytical instruments
ATE303598T1 (de) 1998-07-31 2005-09-15 Tecan Trading Ag Magnetseparator
US6540896B1 (en) 1998-08-05 2003-04-01 Caliper Technologies Corp. Open-Field serial to parallel converter
US6236456B1 (en) 1998-08-18 2001-05-22 Molecular Devices Corporation Optical system for a scanning fluorometer
US6316774B1 (en) 1998-08-18 2001-11-13 Molecular Devices Corporation Optical system for a scanning fluorometer
US6740518B1 (en) * 1998-09-17 2004-05-25 Clinical Micro Sensors, Inc. Signal detection techniques for the detection of analytes
USD439673S1 (en) 1998-10-06 2001-03-27 Sorenson Bioscience Multi-well microcentrifuge tube
US6958392B2 (en) 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
US6572830B1 (en) 1998-10-09 2003-06-03 Motorola, Inc. Integrated multilayered microfludic devices and methods for making the same
WO2000022436A1 (en) 1998-10-13 2000-04-20 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
US6149787A (en) 1998-10-14 2000-11-21 Caliper Technologies Corp. External material accession systems and methods
US6498497B1 (en) 1998-10-14 2002-12-24 Caliper Technologies Corp. Microfluidic controller and detector system with self-calibration
US6948843B2 (en) 1998-10-28 2005-09-27 Covaris, Inc. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
US6086740A (en) 1998-10-29 2000-07-11 Caliper Technologies Corp. Multiplexed microfluidic devices and systems
US5973138A (en) 1998-10-30 1999-10-26 Becton Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
US6441152B1 (en) 1998-12-08 2002-08-27 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
JP2000180455A (ja) 1998-12-15 2000-06-30 Sanuki Kogyo Kk フローインジェクション自動分析装置
US6062261A (en) 1998-12-16 2000-05-16 Lockheed Martin Energy Research Corporation MicrofluIdic circuit designs for performing electrokinetic manipulations that reduce the number of voltage sources and fluid reservoirs
US6887693B2 (en) 1998-12-24 2005-05-03 Cepheid Device and method for lysing cells, spores, or microorganisms
US7914994B2 (en) 1998-12-24 2011-03-29 Cepheid Method for separating an analyte from a sample
US6261431B1 (en) 1998-12-28 2001-07-17 Affymetrix, Inc. Process for microfabrication of an integrated PCR-CE device and products produced by the same
WO2000040334A1 (en) 1999-01-08 2000-07-13 Pe Corporation (Ny) Fiber array for contacting chemical species and methods for using and making same
IT1306964B1 (it) 1999-01-19 2001-10-11 St Microelectronics Srl Circuito a boosting capacitivo per la regolazione della tensione dilettura di riga in memorie non-volatili
US6150119A (en) 1999-01-19 2000-11-21 Caliper Technologies Corp. Optimized high-throughput analytical system
US6416642B1 (en) 1999-01-21 2002-07-09 Caliper Technologies Corp. Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection
US20020019059A1 (en) 1999-01-28 2002-02-14 Calvin Y.H. Chow Devices, systems and methods for time domain multiplexing of reagents
MXPA01007804A (es) 1999-02-02 2003-06-09 Caliper Techn Corp Metodos, dispositivos y sistemas para caracterizar proteinas.
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
ATE469699T1 (de) 1999-02-23 2010-06-15 Caliper Life Sciences Inc Manipulation von mikroteilchen in mikrofluiden systemen
US6143547A (en) 1999-03-02 2000-11-07 Academia Sinica Cell line derived from Penaeus monodon and a method of growing virus using the same
US6749814B1 (en) 1999-03-03 2004-06-15 Symyx Technologies, Inc. Chemical processing microsystems comprising parallel flow microreactors and methods for using same
US6326083B1 (en) 1999-03-08 2001-12-04 Calipher Technologies Corp. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
US6171850B1 (en) 1999-03-08 2001-01-09 Caliper Technologies Corp. Integrated devices and systems for performing temperature controlled reactions and analyses
US6558945B1 (en) 1999-03-08 2003-05-06 Aclara Biosciences, Inc. Method and device for rapid color detection
US6148508A (en) 1999-03-12 2000-11-21 Caliper Technologies Corp. Method of making a capillary for electrokinetic transport of materials
JP2000266760A (ja) 1999-03-17 2000-09-29 Hitachi Ltd 分注装置
JP3988306B2 (ja) 1999-03-25 2007-10-10 東ソー株式会社 自動測定装置
DE60036746T2 (de) 1999-03-25 2008-07-24 Tosoh Corp., Shinnanyo Analysator
US6500323B1 (en) 1999-03-26 2002-12-31 Caliper Technologies Corp. Methods and software for designing microfluidic devices
US6194563B1 (en) 1999-03-26 2001-02-27 Vysis, Inc. Solid phase nucleic acid labeling by transamination
US6783962B1 (en) 1999-03-26 2004-08-31 Upfront Chromatography Particulate material for purification of bio-macromolecules
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6306273B1 (en) 1999-04-13 2001-10-23 Aclara Biosciences, Inc. Methods and compositions for conducting processes in microfluidic devices
US6322683B1 (en) 1999-04-14 2001-11-27 Caliper Technologies Corp. Alignment of multicomponent microfabricated structures
US6942771B1 (en) 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US20040053290A1 (en) 2000-01-11 2004-03-18 Terbrueggen Robert Henry Devices and methods for biochip multiplexing
CA2270106C (en) 1999-04-23 2006-03-14 Yousef Haj-Ahmad Nucleic acid purification and process
US6773676B2 (en) 1999-04-27 2004-08-10 Agilent Technologies, Inc. Devices for performing array hybridization assays and methods of using the same
EP1190252B1 (en) 1999-04-28 2008-12-31 Eidgenössische Technische Hochschule Zürich Polyionic coatings in analytic and sensor devices
US7001725B2 (en) 1999-04-30 2006-02-21 Aclara Biosciences, Inc. Kits employing generalized target-binding e-tag probes
US6555389B1 (en) 1999-05-11 2003-04-29 Aclara Biosciences, Inc. Sample evaporative control
US6458259B1 (en) 1999-05-11 2002-10-01 Caliper Technologies Corp. Prevention of surface adsorption in microchannels by application of electric current during pressure-induced flow
JP3815969B2 (ja) 1999-05-12 2006-08-30 アクララ バイオサイエンシーズ， インコーポレイテッド 微量流体デバイスにおける多重方式蛍光検出
US6838680B2 (en) 1999-05-12 2005-01-04 Aclara Biosciences, Inc. Multiplexed fluorescent detection in microfluidic devices
US6326147B1 (en) 1999-05-13 2001-12-04 The Perkin-Elmer Corporation Methods, apparatus, articles of manufacture, and user interfaces for performing automated biological assay preparation and macromolecule purification
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6592821B1 (en) 1999-05-17 2003-07-15 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
AU770678B2 (en) 1999-05-17 2004-02-26 Caliper Life Sciences, Inc. Focusing of microparticles in microfluidic systems
US6287774B1 (en) 1999-05-21 2001-09-11 Caliper Technologies Corp. Assay methods and system
US6472141B2 (en) 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
WO2001012325A1 (en) 1999-05-28 2001-02-22 Bio/Data Corporation Method and apparatus for directly sampling a fluid for microfiltration
US20040200909A1 (en) 1999-05-28 2004-10-14 Cepheid Apparatus and method for cell disruption
EP1181098B2 (en) 1999-05-28 2011-07-06 Cepheid Cartridge for conducting a chemical reaction
AU782343B2 (en) 1999-05-28 2005-07-21 Cepheid Apparatus and method for analyzing a fluid sample
DE29909529U1 (de) 1999-06-01 1999-08-12 Festo AG & Co, 73734 Esslingen Fluidtechnisches Steuergerät
KR20020006050A (ko) 1999-06-01 2002-01-18 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 멸균 방법
AU5291600A (en) 1999-06-01 2000-12-18 Caliper Technologies Corporation Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
GB9913275D0 (en) 1999-06-08 1999-08-04 Dna Research Instr Limited Sample processing device
US6811668B1 (en) 1999-06-22 2004-11-02 Caliper Life Sciences, Inc. Apparatus for the operation of a microfluidic device
US6706519B1 (en) 1999-06-22 2004-03-16 Tecan Trading Ag Devices and methods for the performance of miniaturized in vitro amplification assays
AU5887000A (en) 1999-06-22 2001-01-09 Tecan Trading Ag Devices and methods for the performance of miniaturized in vitro amplification assays
FR2795426A1 (fr) 1999-06-22 2000-12-29 Commissariat Energie Atomique Support d'analyse biologique a amplification
BR0011982B1 (pt) 1999-06-28 2009-05-05 estrutura elastomérica, método de atuação de uma estrutura elastomérica, método de controle de fluido ou gás através de uma estrutura elastomérica, método de micro-fabricação de uma estrutura elastomérica, uso de uma membrana flexìvel, uso de camadas elastoméricas unidas, uso de um material elastomérico e estrutura elastomérica micro-fabricada.
US7306672B2 (en) 2001-04-06 2007-12-11 California Institute Of Technology Microfluidic free interface diffusion techniques
AU6068300A (en) 1999-07-06 2001-01-22 Caliper Technologies Corporation Microfluidic systems and methods for determining modulator kinetics
US6353475B1 (en) 1999-07-12 2002-03-05 Caliper Technologies Corp. Light source power modulation for use with chemical and biochemical analysis
JP4856831B2 (ja) 1999-07-19 2012-01-18 オルガノン・テクニカ・ベー・ヴエー 磁性粒子を流体と混合するための装置および方法
USD438311S1 (en) 1999-07-28 2001-02-27 Matsushita Electric Industrial Co.,Ltd. Strip for blood test
US6242188B1 (en) 1999-07-30 2001-06-05 Applied Gene Technologies, Inc. Sample processing to release nucleic acids for direct detection
US6337435B1 (en) 1999-07-30 2002-01-08 Bio-Rad Laboratories, Inc. Temperature control for multi-vessel reaction apparatus
US6818060B2 (en) 1999-08-02 2004-11-16 Emerald Biostructures, Inc. Robot for mixing crystallization trial matrices
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
EP1077086B1 (en) 1999-08-18 2004-10-27 Becton Dickinson and Company Stopper-shield assembly
US6495104B1 (en) 1999-08-19 2002-12-17 Caliper Technologies Corp. Indicator components for microfluidic systems
WO2001014931A1 (en) 1999-08-23 2001-03-01 Mitsubishi Chemical Corporation Photopolymerizable composition and photopolymerizable lithographic plate
US6858185B1 (en) 1999-08-25 2005-02-22 Caliper Life Sciences, Inc. Dilutions in high throughput systems with a single vacuum source
US6613581B1 (en) 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
US6613211B1 (en) 1999-08-27 2003-09-02 Aclara Biosciences, Inc. Capillary electrokinesis based cellular assays
US6824663B1 (en) 1999-08-27 2004-11-30 Aclara Biosciences, Inc. Efficient compound distribution in microfluidic devices
US6633785B1 (en) 1999-08-31 2003-10-14 Kabushiki Kaisha Toshiba Thermal cycler and DNA amplifier method
US6752966B1 (en) 1999-09-10 2004-06-22 Caliper Life Sciences, Inc. Microfabrication methods and devices
USD466219S1 (en) 1999-09-13 2002-11-26 Micronic B.V. Carrier for test-tubes
US6906797B1 (en) 1999-09-13 2005-06-14 Aclara Biosciences, Inc. Side light activated microfluid channels
DE19945604A1 (de) 1999-09-23 2003-08-07 Aclara Biosciences Inc Verfahren zur Verbindung von Werkstücken aus Kunststoff und seine Verwendung in der Mikro- und Nanostrukturtechnik
DE19948473A1 (de) 1999-10-08 2001-04-12 Nmi Univ Tuebingen Verfahren und Vorrichtung zum Messen an in einer flüssigen Umgebung befindlichen Zellen
US6221600B1 (en) 1999-10-08 2001-04-24 Board Of Regents, The University Of Texas System Combinatorial oligonucleotide PCR: a method for rapid, global expression analysis
JP2003511682A (ja) 1999-10-08 2003-03-25 カリパー・テクノロジーズ・コープ． 膜透過電圧測定でのネルンスト電圧感知性染料の使用
US6287254B1 (en) 1999-11-02 2001-09-11 W. Jean Dodds Animal health diagnosis
US6272939B1 (en) 1999-10-15 2001-08-14 Applera Corporation System and method for filling a substrate with a liquid sample
US6232072B1 (en) * 1999-10-15 2001-05-15 Agilent Technologies, Inc. Biopolymer array inspection
US6908594B1 (en) 1999-10-22 2005-06-21 Aclara Biosciences, Inc. Efficient microfluidic sealing
USD461906S1 (en) 1999-10-25 2002-08-20 Tuan Hung Pham Diagnostic test card
GB2355717A (en) 1999-10-28 2001-05-02 Amersham Pharm Biotech Uk Ltd DNA isolation method
US6300124B1 (en) 1999-11-02 2001-10-09 Regents Of The University Of Minnesota Device and method to directly control the temperature of microscope slides
US6875619B2 (en) 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
EP1237655A2 (en) 1999-12-09 2002-09-11 Motorola, Inc. Multilayered microfluidic devices for analyte reactions
USD438633S1 (en) 1999-12-21 2001-03-06 Compucyte Corporation Reagent cartridge for treating a sample
USD438632S1 (en) 1999-12-21 2001-03-06 Compucyte Corporation Multi-well reagent cartridge for treating a sample
ATE433499T1 (de) 1999-12-21 2009-06-15 Ortho Clinical Diagnostics Inc Detektion von nukleinsäuren
US6936414B2 (en) 1999-12-22 2005-08-30 Abbott Laboratories Nucleic acid isolation method and kit
US6699713B2 (en) 2000-01-04 2004-03-02 The Regents Of The University Of California Polymerase chain reaction system
EP1244810B1 (en) 2000-01-06 2008-02-20 Caliper Life Sciences, Inc. Methods and systems for monitoring intracellular binding reactions
US6620625B2 (en) 2000-01-06 2003-09-16 Caliper Technologies Corp. Ultra high throughput sampling and analysis systems and methods
US6468761B2 (en) 2000-01-07 2002-10-22 Caliper Technologies, Corp. Microfluidic in-line labeling method for continuous-flow protease inhibition analysis
DE60125713T2 (de) 2000-01-11 2007-11-08 Clinical Micro Sensors, Inc., Pasadena Patrone, die einen Biochip enthält
WO2001051918A1 (en) 2000-01-12 2001-07-19 Ut-Battelle, Llc A microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream
US7037416B2 (en) 2000-01-14 2006-05-02 Caliper Life Sciences, Inc. Method for monitoring flow rate using fluorescent markers
JP3397737B2 (ja) 2000-01-24 2003-04-21 倉敷紡績株式会社 核酸の抽出方法
US6556923B2 (en) 2000-01-26 2003-04-29 Caliper Technologies Corp. Software for high throughput microfluidic systems
US6589729B2 (en) 2000-02-04 2003-07-08 Caliper Technologies Corp. Methods, devices, and systems for monitoring time dependent reactions
US6685813B2 (en) 2000-02-11 2004-02-03 Aclara Biosciences, Inc. Tandem isotachophoresis/zone electrophoresis method and system
CA2399398A1 (en) 2000-02-11 2001-08-16 Aclara Biosciences, Inc. Microfluid device with sample injector and method of use
JP3750460B2 (ja) 2000-02-18 2006-03-01 日立工機株式会社 分注装置及び分注方法
CA2400644C (en) 2000-02-18 2009-07-14 Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US6681616B2 (en) 2000-02-23 2004-01-27 Caliper Technologies Corp. Microfluidic viscometer
US7040144B2 (en) 2000-02-23 2006-05-09 Caliper Life Sciences, Inc. Microfluidic viscometer
AU2001249071B2 (en) 2000-02-23 2005-09-08 Caliper Life Sciences, Inc. Multi-reservoir pressure control system
AU2001249253A1 (en) 2000-03-17 2001-10-03 Aclara Biosciences, Inc. Microfluidic device and system with improved sample handling
US20020012971A1 (en) 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
US6358387B1 (en) 2000-03-27 2002-03-19 Caliper Technologies Corporation Ultra high throughput microfluidic analytical systems and methods
US6927851B2 (en) 2000-03-31 2005-08-09 Neogen Corporation Methods and apparatus to improve the sensitivity and reproducibility of bioluminescent analytical methods
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US6401552B1 (en) * 2000-04-17 2002-06-11 Carlos D. Elkins Centrifuge tube and method for collecting and dispensing mixed concentrated fluid samples
US6733645B1 (en) 2000-04-18 2004-05-11 Caliper Technologies Corp. Total analyte quantitation
USD446306S1 (en) 2000-04-26 2001-08-07 Matsushita Electric Industrial Co., Ltd. Medical information communication apparatus
US7160735B2 (en) 2000-04-28 2007-01-09 Monogram Biosciences, Inc. Tagged microparticle compositions and methods
US6787016B2 (en) 2000-05-01 2004-09-07 Aclara Biosciences, Inc. Dynamic coating with linear polymer mixture for electrophoresis
ATE503209T1 (de) 2000-05-03 2011-04-15 Caliper Life Sciences Inc Herstellungsprozesse für substrate mit mehreren tiefen
US7578976B1 (en) 2000-05-10 2009-08-25 Lawrence Livermore National Security, Llc Sleeve reaction chamber system
AU2001261541B2 (en) 2000-05-11 2004-10-14 Caliper Life Sciences, Inc. Microfluidic devices and methods to regulate hydrodynamic and electrical resistance utilizing bulk viscosity enhancers
ATE409238T1 (de) 2000-05-12 2008-10-15 Caliper Life Sciences Inc Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation
AU2001261767A1 (en) 2000-05-19 2001-12-03 Aclara Biosciences, Inc. Optical alignment in capillary detection using capillary wall scatter
EP1282469B1 (en) * 2000-05-19 2009-01-07 Becton, Dickinson and Company System and method for manipulating magnetically responsive particles in fluid samples to collect dna or rna from a sample
US6672458B2 (en) 2000-05-19 2004-01-06 Becton, Dickinson And Company System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample
JP2004508919A (ja) 2000-05-24 2004-03-25 セルラー　プロセス　ケミストリー　インコーポレイテッド マイクロリアクタを組み入れたモジュラ式化学生産システム
WO2001092569A2 (en) 2000-05-31 2001-12-06 Tepnel Medical Limited Formulation for polymerase chain reaction and vessel containing same
US6520197B2 (en) 2000-06-02 2003-02-18 The Regents Of The University Of California Continuous laminar fluid mixing in micro-electromechanical systems
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
AU2001269896A1 (en) 2000-06-14 2001-12-24 Board Of Regents, The University Of Texas System Systems and methods for cell subpopulation analysis
AU2001269929B2 (en) 2000-06-19 2005-11-03 Caliper Life Sciences, Inc. Methods and devices for enhancing bonded substrate yields and regulating temperature
US7169618B2 (en) 2000-06-28 2007-01-30 Skold Technology Magnetic particles and methods of producing coated magnetic particles
US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US6720187B2 (en) 2000-06-28 2004-04-13 3M Innovative Properties Company Multi-format sample processing devices
AU2001282920A1 (en) * 2000-07-19 2002-01-30 Genisphere Inc Methods for detecting and assaying nucleic acid sequences
DE60106053T2 (de) 2000-07-21 2005-11-17 Aclara BioSciences, Inc., Mountain View Verfahren und vorrichtungen zur kapillarelektrophorese mit einer norbornenhaltigen oberflächenbeschichtung
FR2812088B1 (fr) 2000-07-21 2003-01-24 Abx Sa Dispositif de traitement d'echantillons de produits sanguins
US7004184B2 (en) 2000-07-24 2006-02-28 The Reagents Of The University Of Michigan Compositions and methods for liquid metering in microchannels
AU8095101A (en) 2000-08-02 2002-02-13 Caliper Techn Corp High throughput separations based analysis systems
US20020142618A1 (en) 2000-08-04 2002-10-03 Caliper Technologies Corp. Control of operation conditions within fluidic systems
FR2813207B1 (fr) 2000-08-28 2002-10-11 Bio Merieux Carte reactionnelle et utilisation d'une telle carte
JP3993372B2 (ja) 2000-09-13 2007-10-17 独立行政法人理化学研究所 リアクタの製造方法
AU2001290867A1 (en) 2000-09-14 2002-03-26 Caliper Technologies Corp. Microfluidic devices and methods for performing temperature mediated reactions
EP2299256A3 (en) 2000-09-15 2012-10-10 California Institute Of Technology Microfabricated crossflow devices and methods
US6939451B2 (en) 2000-09-19 2005-09-06 Aclara Biosciences, Inc. Microfluidic chip having integrated electrodes
US7585682B2 (en) 2000-10-03 2009-09-08 Minerva Biotechologies Corporation Magnetic in situ dilution
WO2002028531A1 (en) 2000-10-06 2002-04-11 Protasis Corporation Fluid separation conduit cartridge with encryption capability
US6623860B2 (en) 2000-10-10 2003-09-23 Aclara Biosciences, Inc. Multilevel flow structures
USD463031S1 (en) 2000-10-11 2002-09-17 Aclara Biosciences, Inc. Microvolume sample plate
EP1325459A4 (en) 2000-10-13 2010-09-01 Irm Llc HIGH EFFICIENCY PROCESSING SYSTEM AND METHOD OF USE
US6375185B1 (en) 2000-10-20 2002-04-23 Gamemax Corporation Paper currency receiving control assembly for currency-coin exchange machine
JP2004513342A (ja) 2000-10-31 2004-04-30 カリパー・テクノロジーズ・コープ． 現場で材料を濃縮するミクロ流体方法、装置及びシステム
US7514046B2 (en) 2000-10-31 2009-04-07 Caliper Life Sciences, Inc. Methods and systems for processing microscale devices for reuse
US7105304B1 (en) 2000-11-07 2006-09-12 Caliper Life Sciences, Inc. Pressure-based mobility shift assays
US8900811B2 (en) 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
EP1345698A4 (en) 2000-11-16 2006-05-17 Fluidigm Corp MICROFLUID DEVICES FOR THE INTRODUCTION AND DISPOSAL OF FLUIDS FROM MICROFLUID SYSTEMS
US20050202470A1 (en) 2000-11-16 2005-09-15 Caliper Life Sciences, Inc. Binding assays using molecular melt curves
CH695544A5 (de) 2000-11-17 2006-06-30 Tecan Trading Ag Vorrichtung zur Abgabe bzw. Aufnahme/Abgabe von Flüssigkeitsproben.
USD468437S1 (en) 2000-11-21 2003-01-07 Acon Laboratories, Inc. Test platform
US6521188B1 (en) 2000-11-22 2003-02-18 Industrial Technology Research Institute Microfluidic actuator
SE0004296D0 (sv) 2000-11-23 2000-11-23 Gyros Ab Device and method for the controlled heating in micro channel systems
US7024281B1 (en) 2000-12-11 2006-04-04 Callper Life Sciences, Inc. Software for the controlled sampling of arrayed materials
US20040043479A1 (en) 2000-12-11 2004-03-04 Briscoe Cynthia G. Multilayerd microfluidic devices for analyte reactions
US6382254B1 (en) 2000-12-12 2002-05-07 Eastman Kodak Company Microfluidic valve and method for controlling the flow of a liquid
KR100881923B1 (ko) 2000-12-27 2009-02-04 다이나박스 테크놀로지 코퍼레이션 면역자극 폴리뉴클레오티드 및 그것의 사용 방법
US6453928B1 (en) 2001-01-08 2002-09-24 Nanolab Ltd. Apparatus, and method for propelling fluids
JP4505776B2 (ja) * 2001-01-19 2010-07-21 凸版印刷株式会社 遺伝子検出システム、これを備えた遺伝子検出装置、検出方法、並びに遺伝子検出用チップ
US6878755B2 (en) 2001-01-22 2005-04-12 Microgen Systems, Inc. Automated microfabrication-based biodetector
JP3548858B2 (ja) 2001-01-22 2004-07-28 独立行政法人産業技術総合研究所 流量の制御方法及びそれに用いるマイクロバルブ
EP1355823A4 (en) 2001-01-29 2005-04-20 Caliper Life Sciences Inc NON-MECHANICAL VALVES FOR FLUID SYSTEMS
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7670559B2 (en) 2001-02-15 2010-03-02 Caliper Life Sciences, Inc. Microfluidic systems with enhanced detection systems
US6432695B1 (en) 2001-02-16 2002-08-13 Institute Of Microelectronics Miniaturized thermal cycler
US6509186B1 (en) 2001-02-16 2003-01-21 Institute Of Microelectronics Miniaturized thermal cycler
US6720148B1 (en) 2001-02-22 2004-04-13 Caliper Life Sciences, Inc. Methods and systems for identifying nucleotides by primer extension
US7867776B2 (en) 2001-03-02 2011-01-11 Caliper Life Sciences, Inc. Priming module for microfluidic chips
WO2002072264A1 (en) 2001-03-09 2002-09-19 Biomicro Systems, Inc. Method and system for microfluidic interfacing to arrays
US7150999B1 (en) 2001-03-09 2006-12-19 Califer Life Sciences, Inc. Process for filling microfluidic channels
US6576459B2 (en) 2001-03-23 2003-06-10 The Regents Of The University Of California Sample preparation and detection device for infectious agents
US20140227710A1 (en) 2001-03-28 2014-08-14 Handylab, Inc. Moving microdroplets in a microfluidic device
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7192557B2 (en) 2001-03-28 2007-03-20 Handylab, Inc. Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
US6575188B2 (en) 2001-07-26 2003-06-10 Handylab, Inc. Methods and systems for fluid control in microfluidic devices
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US7270786B2 (en) 2001-03-28 2007-09-18 Handylab, Inc. Methods and systems for processing microfluidic samples of particle containing fluids
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
JP2002355038A (ja) 2001-03-29 2002-12-10 Japan Science & Technology Corp 遺伝子解析方法およびその解析装置
US20020143297A1 (en) 2001-03-30 2002-10-03 Becton, Dickinson And Company Adaptor for use with point-of-care testing cartridge
US6960437B2 (en) 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
USD470595S1 (en) 2001-04-10 2003-02-18 Andrea Crisanti Assay device
USD500142S1 (en) 2001-04-10 2004-12-21 Andrea Crisanti Assay device
US7440684B2 (en) 2001-04-12 2008-10-21 Spaid Michael A Method and apparatus for improved temperature control in microfluidic devices
US6588625B2 (en) 2001-04-24 2003-07-08 Abbott Laboratories Sample handling system
US20020155010A1 (en) 2001-04-24 2002-10-24 Karp Christoph D. Microfluidic valve with partially restrained element
EP3231445A1 (en) 2001-05-18 2017-10-18 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
USD495805S1 (en) 2001-05-25 2004-09-07 Umedik, Inc. Assay device
US7723123B1 (en) 2001-06-05 2010-05-25 Caliper Life Sciences, Inc. Western blot by incorporating an affinity purification zone
US20020187557A1 (en) 2001-06-07 2002-12-12 Hobbs Steven E. Systems and methods for introducing samples into microfluidic devices
US7025774B2 (en) 2001-06-12 2006-04-11 Pelikan Technologies, Inc. Tissue penetration device
US6977163B1 (en) 2001-06-13 2005-12-20 Caliper Life Sciences, Inc. Methods and systems for performing multiple reactions by interfacial mixing
DE10128894A1 (de) 2001-06-15 2002-12-19 Basf Ag Verfahren zur schmutzablösungsfördernden Behandlung von Oberflächen textiler und nicht-textiler Materialien
US6859698B2 (en) 2001-06-21 2005-02-22 Snap-On Incorporated Detachable cartridge unit and auxiliary unit for function expansion of a data processing system
US20030211517A1 (en) 2001-06-22 2003-11-13 Carulli John P. Gp354 nucleic acids and polypeptides
US6709857B2 (en) 2001-06-26 2004-03-23 Becton, Dickinson And Company System and method for optically monitoring the concentration of a gas in a sample vial using photothermal spectroscopy to detect sample growth
US6514750B2 (en) 2001-07-03 2003-02-04 Pe Corporation (Ny) PCR sample handling device
US6762049B2 (en) 2001-07-05 2004-07-13 Institute Of Microelectronics Miniaturized multi-chamber thermal cycler for independent thermal multiplexing
WO2003006964A1 (en) 2001-07-12 2003-01-23 Aclara Biosciences, Inc. Submersible light-directing member for material excitation in microfluidic devices
CA2450343C (en) 2001-07-16 2013-12-17 Idaho Technology, Inc. Thermal cycling system and method of use
US7023007B2 (en) 2001-07-17 2006-04-04 Caliper Life Sciences, Inc. Methods and systems for alignment of detection optics
US7270959B2 (en) 2001-07-25 2007-09-18 Oakville Hong Kong Company Limited Specimen collection container
US6766817B2 (en) 2001-07-25 2004-07-27 Tubarc Technologies, Llc Fluid conduction utilizing a reversible unsaturated siphon with tubarc porosity action
AU2002367840A1 (en) * 2001-07-26 2003-11-17 Motorola, Inc. System and methods for mixing within a microfluidic device
ES2683698T3 (es) 2001-07-26 2018-09-27 Handylab, Inc. Métodos y sistemas para procesamiento microfluídico
US20060062696A1 (en) 2001-07-27 2006-03-23 Caliper Life Sciences, Inc. Optimized high throughput analytical systems
US7060171B1 (en) 2001-07-31 2006-06-13 Caliper Life Sciences, Inc. Methods and systems for reducing background signal in assays
JP2003047840A (ja) 2001-08-06 2003-02-18 Yamatake Corp マイクロリアクタ
JP2003047839A (ja) 2001-08-06 2003-02-18 Yamatake Corp マイクロリアクタ
US6640981B2 (en) 2001-08-14 2003-11-04 3088081 Canada Inc. Modular test tube rack
USD482796S1 (en) 2001-09-11 2003-11-25 Sysmex Corporation Sample analyzer
USD512155S1 (en) 2001-09-12 2005-11-29 Techno Medica Co., Ltd. Automatic blood sampling tube preparation apparatus
US20030059823A1 (en) 2001-09-21 2003-03-27 Juki Corporation Hybridization apparatus and method for detecting nucleic acid in sample using the same
JP3996416B2 (ja) 2001-09-21 2007-10-24 Ｊｕｋｉ株式会社 核酸ハイブリダイゼーションにおけるｂ／ｆ分離方法
USD467349S1 (en) 2001-09-28 2002-12-17 Orasure Technologies, Inc. Analyzer
USD467348S1 (en) 2001-10-15 2002-12-17 Kimberly-Clark Worldwide, Inc. Diagnostic test carrier
WO2003047712A2 (en) 2001-10-18 2003-06-12 The Board Of Trustees Of The University Of Illinois Hybrid microfluidic and nanofluidic system
US20030156991A1 (en) 2001-10-23 2003-08-21 William Marsh Rice University Optomechanically-responsive materials for use as light-activated actuators and valves
US7338760B2 (en) 2001-10-26 2008-03-04 Ntu Ventures Private Limited Sample preparation integrated chip
US7247274B1 (en) 2001-11-13 2007-07-24 Caliper Technologies Corp. Prevention of precipitate blockage in microfluidic channels
US6750661B2 (en) 2001-11-13 2004-06-15 Caliper Life Sciences, Inc. Method and apparatus for controllably effecting samples using two signals
US7069952B1 (en) 2001-11-14 2006-07-04 Caliper Life Sciences, Inc. Microfluidic devices and methods of their manufacture
DE10156790A1 (de) 2001-11-19 2003-06-18 Chemagen Biopolymer Technologi Vorrichtung und Verfahren zum Behandeln von Magnetpartikeln
US20030099954A1 (en) 2001-11-26 2003-05-29 Stefan Miltenyi Apparatus and method for modification of magnetically immobilized biomolecules
US7635588B2 (en) 2001-11-29 2009-12-22 Applied Biosystems, Llc Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
US7118910B2 (en) 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
US6960235B2 (en) 2001-12-05 2005-11-01 The Regents Of The University Of California Chemical microreactor and method thereof
JP2003185584A (ja) 2001-12-18 2003-07-03 Fuji Photo Film Co Ltd スキャナ
DE10163476A1 (de) 2001-12-21 2003-10-30 Siemens Ag Anordnung zur Trennung einer Komponente aus einem Fluid
US20030127327A1 (en) 2002-01-04 2003-07-10 Kurnik Ronald T. Microfluidic device and method for improved sample handling
US7410615B2 (en) 2002-01-24 2008-08-12 Perkinelmer Las, Inc. Precision liquid dispensing system
US7205154B2 (en) 2002-01-31 2007-04-17 Agilent Technologies, Inc. Calibrating array scanners
US7288228B2 (en) 2002-02-12 2007-10-30 Gilson, Inc. Sample injection system
US6819027B2 (en) 2002-03-04 2004-11-16 Cepheid Method and apparatus for controlling ultrasonic transducer
US7101467B2 (en) 2002-03-05 2006-09-05 Caliper Life Sciences, Inc. Mixed mode microfluidic systems
EP2497564B1 (en) 2002-03-05 2014-05-14 Caliper Life Sciences, Inc. Electrophoretic separation in a microfluidic channel network
US7303727B1 (en) 2002-03-06 2007-12-04 Caliper Life Sciences, Inc Microfluidic sample delivery devices, systems, and methods
US7195986B1 (en) 2002-03-08 2007-03-27 Caliper Life Sciences, Inc. Microfluidic device with controlled substrate conductivity
KR100450818B1 (ko) 2002-03-09 2004-10-01 삼성전자주식회사 다챔버 ｐｃｒ 칩
US7252928B1 (en) 2002-03-12 2007-08-07 Caliper Life Sciences, Inc. Methods for prevention of surface adsorption of biological materials to capillary walls in microchannels
US7223371B2 (en) 2002-03-14 2007-05-29 Micronics, Inc. Microfluidic channel network device
JP2004003989A (ja) 2002-03-15 2004-01-08 Affymetrix Inc 生物学的物質の走査のためのシステム、方法、および製品
DE10212761B4 (de) 2002-03-22 2009-12-31 Eppendorf Ag Mikrotiterplatte
KR100830926B1 (ko) 2002-03-27 2008-05-22 일렉트론 바이오 (주) 바이오 디스크 및 바이오 드라이버 장치 및 이들을 이용한분석방법
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
AU2003228395B2 (en) 2002-04-02 2006-12-21 Caliper Life Sciences, Inc. Methods and apparatus for separation and isolation of components from a biological sample
USD472324S1 (en) 2002-04-05 2003-03-25 Charles River Laboratories, Inc. Cuvette
JP2003299485A (ja) 2002-04-10 2003-10-21 Sekisui Chem Co Ltd 温度制御型マイクロリアクター及びマイクロリアクターシステム
AU2003228514A1 (en) 2002-04-11 2003-10-27 Sequenom, Inc. Methods and devices for performing chemical reactions on a solid support
US8241883B2 (en) 2002-04-24 2012-08-14 Caliper Life Sciences, Inc. High throughput mobility shift
EP2500076B1 (en) 2002-04-26 2017-11-01 Abbott Laboratories Structure and method for handling magnetic particles in biological assays
US6905612B2 (en) 2003-03-21 2005-06-14 Hanuman Llc Plasma concentrate apparatus and method
JP3972189B2 (ja) 2002-05-10 2007-09-05 日本パルスモーター株式会社 着脱自在なカートリッジラック構造を備える分注装置
USD474279S1 (en) 2002-05-15 2003-05-06 Monogen, Inc. Specimen processing instrument
JP3839349B2 (ja) 2002-05-15 2006-11-01 株式会社堀場製作所 化学発光酵素免疫測定装置
DE60316938T2 (de) 2002-05-17 2008-07-17 Gen-Probe Inc., San Diego Probenträger mit lösbarer sperreinrichtung
EP1506413B1 (en) * 2002-05-17 2016-07-06 Becton Dickinson and Company Automated system for isolating, amplyifying and detecting a target nucleic acid sequence
AU2003239485B2 (en) 2002-05-17 2008-11-20 Gen-Probe Incorporated Sample carrier having sample tube blocking means and drip shield for use therewith
AU2003222704A1 (en) 2002-05-24 2003-12-12 Epr Labautomation Ag Method and device for dosing small volumes of liquid
US7161356B1 (en) 2002-06-05 2007-01-09 Caliper Life Sciences, Inc. Voltage/current testing equipment for microfluidic devices
USD480814S1 (en) 2002-06-11 2003-10-14 Diversa Corporation Gigamatrix holding tray
US7208125B1 (en) 2002-06-28 2007-04-24 Caliper Life Sciences, Inc Methods and apparatus for minimizing evaporation of sample materials from multiwell plates
AU2003249231A1 (en) 2002-07-15 2004-02-02 Phynexus, Inc. Low dead volume extraction column devices
EP1539352B1 (en) 2002-07-23 2009-12-23 Protedyne Corporation Liquid handling tool having hollow plunger
US7214348B2 (en) 2002-07-26 2007-05-08 Applera Corporation Microfluidic size-exclusion devices, systems, and methods
CA2492613A1 (en) 2002-07-26 2004-02-05 Applera Corporation Microfluidic size-exclusion devices, systems, and methods
US7041258B2 (en) 2002-07-26 2006-05-09 Applera Corporation Micro-channel design features that facilitate centripetal fluid transfer
DE10236029A1 (de) 2002-08-02 2004-02-19 Cybio Systems Gmbh Einrichtung zum Dispensieren und Beobachten der Lumineszenz von Einzelproben in Multiprobenanordnungen
US20040053315A1 (en) 2002-08-12 2004-03-18 Caliper Technologies Corp. Methods and systems for monitoring molecular interactions
GB0219457D0 (en) 2002-08-21 2002-09-25 Amersham Biosciences Uk Ltd Fluorescence reference plate
US7001853B1 (en) 2002-08-30 2006-02-21 Caliper Life Sciences, Inc. Flow control of photo-polymerizable resin
USD516221S1 (en) 2002-09-09 2006-02-28 Meso Scale Technologies, Llc. Diagnostic instrument
ITTO20020808A1 (it) 2002-09-17 2004-03-18 St Microelectronics Srl Dispositivo integrato di analisi del dna.
JP3756477B2 (ja) * 2002-09-17 2006-03-15 横河電機株式会社 デンドリマーによる核酸またはタンパク質の抽出方法およびデンドリマー組成物
USD484989S1 (en) 2002-09-20 2004-01-06 Dade Behring Inc. Multi-well liquid container
TW590982B (en) 2002-09-27 2004-06-11 Agnitio Science & Technology I Micro-fluid driving device
US6730883B2 (en) 2002-10-02 2004-05-04 Stratagene Flexible heating cover assembly for thermal cycling of samples of biological material
WO2004034028A2 (en) 2002-10-09 2004-04-22 The Board Of Trustees Of The University Of Illinois Microfluidic systems and components
US7932098B2 (en) 2002-10-31 2011-04-26 Hewlett-Packard Development Company, L.P. Microfluidic system utilizing thin-film layers to route fluid
US7122384B2 (en) 2002-11-06 2006-10-17 E. I. Du Pont De Nemours And Company Resonant light scattering microparticle methods
US20040141887A1 (en) * 2002-11-08 2004-07-22 Irm, Llc Apparatus and methods to process substrate surface features
JP2004170159A (ja) 2002-11-18 2004-06-17 Hitachi Koki Co Ltd 自動分注装置
CA2506714A1 (en) * 2002-11-26 2004-06-10 University Of Massachusetts Delivery of sirnas
USD491276S1 (en) 2002-12-09 2004-06-08 Babette Langille Plastic diagnostic card
USD491272S1 (en) 2002-12-13 2004-06-08 Immunivest Corporation Autoprep instrument
DE60239968D1 (de) 2002-12-13 2011-06-16 Dhr Finland Oy Sche
WO2004055492A2 (en) 2002-12-13 2004-07-01 Aclara Biosciences, Inc. Closed-loop control of electrokinetic processes in microfludic devices based on optical readings
USD491273S1 (en) 2002-12-19 2004-06-08 3M Innovative Properties Company Hybridization cartridge
SE0203781D0 (sv) 2002-12-19 2002-12-19 Alphahelix Ab Holder and method for cooling or heating samples
US20050042639A1 (en) 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
US20060094108A1 (en) 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
WO2004083443A1 (en) 2002-12-20 2004-09-30 Caliper Life Sciences, Inc. Single molecule amplification and detection of dna
US7850912B2 (en) 2003-05-14 2010-12-14 Dako Denmark A/S Method and apparatus for automated pre-treatment and processing of biological samples
US7648678B2 (en) 2002-12-20 2010-01-19 Dako Denmark A/S Method and system for pretreatment of tissue slides
US8676383B2 (en) 2002-12-23 2014-03-18 Applied Biosystems, Llc Device for carrying out chemical or biological reactions
WO2004061418A2 (en) 2002-12-26 2004-07-22 Meso Scale Technologies, Llc. Assay cartridges and methods of using the same
US20060113190A1 (en) 2002-12-27 2006-06-01 Kurnik Ronald T Microfluidic device and method for improved sample handling
EP2404676A1 (en) 2002-12-30 2012-01-11 The Regents of the University of California Microfluidic Control Structures
US20070269891A9 (en) * 2003-01-13 2007-11-22 Yasunobu Tanaka Solid surface with immobilized degradable cationic polymer for transfecting eukaryotic cells
US7419638B2 (en) 2003-01-14 2008-09-02 Micronics, Inc. Microfluidic devices for fluid manipulation and analysis
US6964747B2 (en) 2003-01-21 2005-11-15 Bioarray Solutions, Ltd. Production of dyed polymer microparticles
US7049558B2 (en) 2003-01-27 2006-05-23 Arcturas Bioscience, Inc. Apparatus and method for heating microfluidic volumes and moving fluids
US7338637B2 (en) 2003-01-31 2008-03-04 Hewlett-Packard Development Company, L.P. Microfluidic device with thin-film electronic devices
JP4021335B2 (ja) 2003-01-31 2007-12-12 ユニバーサル・バイオ・リサーチ株式会社 監視機能付分注装置および分注装置の監視方法
CN1767897B (zh) 2003-02-05 2011-03-02 伊库姆公司 试样处理细管
US20040157220A1 (en) 2003-02-10 2004-08-12 Purnima Kurnool Methods and apparatus for sample tracking
KR100959101B1 (ko) 2003-02-20 2010-05-25 삼성전자주식회사 Ｐｃｒ 반응기 및 ｐｃｒ 반응기의 입구와 출구의 개폐를조절하는 방법
WO2004076056A2 (en) 2003-02-26 2004-09-10 Lake Shore Cryotronics Inc. Microfluidic chemical reactor for the manufacture of chemically-produced nanoparticles
JP4129864B2 (ja) 2003-03-24 2008-08-06 農工大ティー・エル・オー株式会社 磁気微粒子の磁気分離装置
JP2004305009A (ja) 2003-04-02 2004-11-04 Hitachi Ltd 核酸増幅装置及び核酸増幅方法
US20050058577A1 (en) 2003-04-08 2005-03-17 Irm, Llc Material removal and dispensing devices, systems, and methods
CA2515850A1 (en) 2003-04-14 2004-10-28 Caliper Life Sciences, Inc. Reduction of migration shift assay interference
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
GB2401237A (en) 2003-04-28 2004-11-03 Hewlett Packard Development Co Data transfer arrangement for disaster recovery
US7148043B2 (en) 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
EP1620204A1 (en) 2003-05-09 2006-02-01 Caliper Life Sciences, Inc. Automated sample analysis
US7038472B1 (en) 2003-05-12 2006-05-02 Caliper Life Sciences, Inc. Methods and systems for measuring internal dimensions of microscale structures
US7374949B2 (en) 2003-05-29 2008-05-20 Bayer Healthcare Llc Diagnostic test strip for collecting and detecting an analyte in a fluid sample
US7055695B2 (en) 2003-06-02 2006-06-06 Caliper Life Sciencee, Inc. Container providing a controlled hydrated environment
JP2005009870A (ja) 2003-06-16 2005-01-13 Fuji Photo Film Co Ltd 分析装置の吸引方法
JP4531698B2 (ja) 2003-07-17 2010-08-25 三菱化学メディエンス株式会社 自動測定用カートリッジおよびそれを用いる測定装置
US7381370B2 (en) 2003-07-18 2008-06-03 Dade Behring Inc. Automated multi-detector analyzer
EP1648622A4 (en) * 2003-07-21 2009-11-11 Dendritic Nanotechnologies Inc STABILIZED AND CHEMICALLY FUNCTIONALIZED NANOPARTICLES
USD508999S1 (en) 2003-07-24 2005-08-30 Biomerieux, Inc. Sample testing machine
USD500363S1 (en) 2003-07-24 2004-12-28 Biomerieux Inc. Sample holder
EP2402089A1 (en) 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US7744817B2 (en) 2003-08-11 2010-06-29 Sakura Finetek U.S.A., Inc. Manifold assembly
US7413712B2 (en) 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
US7347617B2 (en) * 2003-08-19 2008-03-25 Siemens Healthcare Diagnostics Inc. Mixing in microfluidic devices
USD499813S1 (en) 2003-08-22 2004-12-14 As.Pire Bioresearch Inc. Assay testing device
JP2005065607A (ja) 2003-08-26 2005-03-17 Hitachi Ltd 遺伝子処理チップおよび遺伝子処理装置
USD515707S1 (en) 2003-09-01 2006-02-21 Matsushita Electric Industrial Co., Ltd. Fluorescent reader
EP1660881A2 (en) 2003-09-05 2006-05-31 Caliper Life Sciences, Inc. Analyte injection system
US7501094B2 (en) * 2003-09-15 2009-03-10 Syngenta Limited Preparation and characterization of formulations in a high throughput mode
US20050220675A1 (en) 2003-09-19 2005-10-06 Reed Mark T High density plate filler
CA2539131A1 (en) 2003-09-19 2005-03-31 University Of Rochester Diagnostic system for otolaryngologic pathogens and use thereof
US20050069898A1 (en) 2003-09-25 2005-03-31 Cepheid Lyophilized beads containing mannitol
JP2007506439A (ja) * 2003-09-26 2007-03-22 データスコープ・インベストメント・コーポレイション 少量の核酸を合成する方法
DE10344700A1 (de) 2003-09-26 2005-04-14 Hirschmann Laborgeräte GmbH & Co. KG Mehrkanal-Pipettiervorrichtung
US8554372B2 (en) 2003-09-29 2013-10-08 Leica Biosystems Melbourne Pty Ltd System and method for histological tissue specimen processing
USD528215S1 (en) 2003-09-30 2006-09-12 Biacore Ab Chip carrier for biosensor
US7039527B2 (en) 2003-10-01 2006-05-02 Caliper Life Sciences, Inc. Method for measuring diffusivities of compounds using microchips
NL1024578C2 (nl) 2003-10-21 2005-04-22 Univ Delft Tech Inrichting voor het uitvoeren van een reactie.
AU2004291066C1 (en) 2003-11-12 2011-08-04 Van Lue Veterinary Surgical, Llc Trocars and trocar assemblies
US7754500B2 (en) 2003-11-21 2010-07-13 Anp Technologies, Inc. Asymmetrically branched polymer conjugates and microarray assays
EP1541237A3 (en) 2003-12-10 2006-02-01 Samsung Electronics Co., Ltd. Polymerase chain reaction (pcr) module and multiple pcr system using the same
JP4595446B2 (ja) 2003-12-12 2010-12-08 Ｄｉｃ株式会社 核酸増幅方法
JP2005176613A (ja) * 2003-12-16 2005-07-07 Yokogawa Electric Corp デンドリマー利用のｄｎａ抽出方法およびバイオチップ
US7122799B2 (en) 2003-12-18 2006-10-17 Palo Alto Research Center Incorporated LED or laser enabled real-time PCR system and spectrophotometer
EP1697732A1 (en) 2003-12-23 2006-09-06 Caliper Life Sciences, Inc. Analyte injection system
JP4750412B2 (ja) 2003-12-25 2011-08-17 モレックス インコーポレイテド 生体由来分子、ダイオキシン類及び内分泌撹乱物質検出装置及び当該装置を使用した検出方法
KR100750586B1 (ko) 2003-12-26 2007-08-20 한국전자통신연구원 미소유체 가열 시스템
JP4732683B2 (ja) 2003-12-29 2011-07-27 ユニバーサル・バイオ・リサーチ株式会社 標的物質の検出方法
US7099778B2 (en) 2003-12-30 2006-08-29 Caliper Life Sciences, Inc. Method for determining diffusivity and molecular weight in a microfluidic device
WO2005072353A2 (en) 2004-01-25 2005-08-11 Fluidigm Corporation Crystal forming devices and systems and methods for making and using the same
CN1942590B (zh) 2004-02-18 2012-09-05 周小川 多元化学和生化反应的流体装置和方法
WO2005082043A2 (en) * 2004-02-24 2005-09-09 Thermal Gradient Thermal cycling device
US20050196321A1 (en) 2004-03-03 2005-09-08 Zhili Huang Fluidic programmable array devices and methods
USD517554S1 (en) 2004-03-05 2006-03-21 Seiko Epson Corporation Film scanner
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
JP2007529753A (ja) 2004-03-19 2007-10-25 エスピール・カハット ナノ量の液体を吸引、操作、混合、及び分注する装置
JP3858029B2 (ja) 2004-03-22 2006-12-13 株式会社アイディエス 試験管の検知装置
WO2005094981A1 (en) 2004-03-29 2005-10-13 Agilent Technologies, Inc. Cyclic pcr system
JP2005291954A (ja) 2004-03-31 2005-10-20 Olympus Corp 使い捨て試薬パックとその試薬パックを用いる分析装置
US8900856B2 (en) 2004-04-08 2014-12-02 Biomatrica, Inc. Integration of sample storage and sample management for life science
WO2005102526A1 (en) 2004-04-09 2005-11-03 Research Think Tank, Inc. Devices and methods for collection, storage and transportation of biological specimens
EP1745153B1 (en) 2004-05-03 2015-09-30 Handylab, Inc. Processing polynucleotide-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
JP4784508B2 (ja) 2004-05-07 2011-10-05 コニカミノルタエムジー株式会社 検査用マイクロリアクタおよび検査装置ならびに検査方法
GB2414059B (en) 2004-05-10 2008-06-11 E2V Tech Uk Ltd Microfluidic device
JP3952036B2 (ja) 2004-05-13 2007-08-01 コニカミノルタセンシング株式会社 マイクロ流体デバイス並びに試液の試験方法および試験システム
AU2005246404A1 (en) 2004-05-21 2005-12-01 Caliper Life Sciences, Inc. Automated system for handling microfluidic devices
US7553671B2 (en) 2004-05-25 2009-06-30 Vertex Pharmaceuticals, Inc. Modular test tube rack
WO2005116202A1 (ja) 2004-05-27 2005-12-08 Universal Bio Research Co., Ltd. 反応容器、反応測定装置、および液回転処理装置
US7311794B2 (en) 2004-05-28 2007-12-25 Wafergen, Inc. Methods of sealing micro wells
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
AU2005252242A1 (en) 2004-06-07 2005-12-22 Irm Llc Dispensing systems, software, and related methods
WO2006004929A2 (en) 2004-06-28 2006-01-12 Boise State University Electrochemical deposition method utilizing microdroplets of solution
US20060002817A1 (en) 2004-06-30 2006-01-05 Sebastian Bohm Flow modulation devices
US7480042B1 (en) 2004-06-30 2009-01-20 Applied Biosystems Inc. Luminescence reference standards
US8965710B2 (en) 2004-07-02 2015-02-24 The United States Of America, As Represented By The Secretary Of The Navy Automated sample-to-microarray apparatus and method
JP4440020B2 (ja) 2004-07-09 2010-03-24 株式会社神戸製鋼所 マイクロ反応器及びその製造方法
USD523153S1 (en) 2004-07-23 2006-06-13 Hitachi High-Technologies Corporation Main part for immunity analysis machine
EP1621890A1 (en) 2004-07-26 2006-02-01 bioMerieux B.V. Device and method for separating, mixing and concentrating magnetic particles with a fluid and use thereof in purification methods
US7585663B2 (en) 2004-08-26 2009-09-08 Applied Biosystems, Llc Thermal device, system, and method, for fluid processing device
US8053214B2 (en) 2004-09-09 2011-11-08 Microfluidic Systems, Inc. Apparatus and method of extracting and optically analyzing an analyte from a fluid-based sample
CN101073002B (zh) 2004-09-15 2012-08-08 英特基因有限公司 微流体装置
KR100668304B1 (ko) 2004-09-16 2007-01-12 삼성전자주식회사 Ｐｃｒ 혼합액을 ｐｃｒ 칩의 각 ｐｃｒ 채널에 주입하기위한 장치 및 상기 장치를 포함하는 ｐｃｒ 칩 유니트
CN101031801B (zh) 2004-09-30 2010-12-01 爱科来株式会社 薄膜加热器和分析用具
USD548841S1 (en) 2004-10-15 2007-08-14 Microsulis, Ltd Electrical equipment for ablative treatment
JPWO2006043642A1 (ja) 2004-10-20 2008-05-22 株式会社荏原製作所 流体反応装置
US20060094004A1 (en) 2004-10-28 2006-05-04 Akihisa Nakajima Micro-reactor, biological material inspection device, and microanalysis system
JP2006145458A (ja) 2004-11-24 2006-06-08 Yaskawa Electric Corp 分注装置
US7785868B2 (en) 2004-12-02 2010-08-31 Microfluidic Systems, Inc. Apparatus to automatically lyse a sample
US7727477B2 (en) 2004-12-10 2010-06-01 Bio-Rad Laboratories, Inc. Apparatus for priming microfluidics devices with feedback control
JP4573243B2 (ja) 2004-12-15 2010-11-04 小林クリエイト株式会社 試験管用トレイ
AU2005316501B2 (en) * 2004-12-17 2010-10-21 Nitto Denko Corporation Immobilized degradable cationic polymer for transfecting eukaryotic cells
US20060165558A1 (en) 2004-12-21 2006-07-27 Thomas Witty Cartridge for diagnostic assays
US7315376B2 (en) 2005-01-07 2008-01-01 Advanced Molecular Systems, Llc Fluorescence detection system
US20060183216A1 (en) 2005-01-21 2006-08-17 Kalyan Handique Containers for liquid storage and delivery with application to microfluidic devices
US7964380B2 (en) * 2005-01-21 2011-06-21 Argylia Technologies Nanoparticles for manipulation of biopolymers and methods of thereof
DE102005004664B4 (de) 2005-02-02 2007-06-21 Chemagen Biopolymer-Technologie Aktiengesellschaft Vorrichtung und Verfahren und Verwendung zum Abtrennen von magnetischen oder magnetisierbaren Partikeln aus einer Flüssigkeit sowie deren Verwendungen
US20080069729A1 (en) 2005-02-16 2008-03-20 Mcneely Michael R Liquid Valving Using Reactive or Responsive Materials
JP4982387B2 (ja) 2005-02-18 2012-07-25 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッド 微生物のゲノムｄｎａを同定するデバイスおよび方法
USD535403S1 (en) 2005-02-25 2007-01-16 Fuji Photo Film Co., Ltd. Component extractor for biochemistry
JP2008532048A (ja) 2005-03-07 2008-08-14 ノブックス システムズ インコーポレーテッド 自動分析器
ZA200707354B (en) 2005-03-08 2009-04-29 Authentix Inc Microfluidic device for identification, quantification, and authentication of latent markers
EP1861721B1 (en) 2005-03-10 2017-05-03 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
US20060228734A1 (en) 2005-03-18 2006-10-12 Applera Corporation Fluid processing device with captured reagent beads
US7507575B2 (en) 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
US9097723B2 (en) 2005-04-01 2015-08-04 Caliper Life Sciences, Inc. Method and apparatus for performing peptide digestion on a microfluidic device
JP4525427B2 (ja) 2005-04-01 2010-08-18 パナソニック株式会社 分析ディスク、並びに分析ディスクおよび分析装置の検査方法
US20060246493A1 (en) 2005-04-04 2006-11-02 Caliper Life Sciences, Inc. Method and apparatus for use in temperature controlled processing of microfluidic samples
CA112854S (en) 2005-04-10 2007-03-27 Akubio Ltd Microbalance analyser cartridge
AU2006240184A1 (en) 2005-04-12 2006-11-02 Caliper Life Sciences, Inc. Compact optical detection system for a microfluidic devices
US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples
KR100941416B1 (ko) 2005-04-30 2010-02-11 삼성전자주식회사 바이오 디스크 및 바이오 드라이버 장치 및 이들을 이용한분석방법
US7300631B2 (en) 2005-05-02 2007-11-27 Bioscale, Inc. Method and apparatus for detection of analyte using a flexural plate wave device and magnetic particles
US20070259348A1 (en) 2005-05-03 2007-11-08 Handylab, Inc. Lyophilized pellets
USD566291S1 (en) 2005-05-03 2008-04-08 Handylab, Inc. Microfluidic cartridge
USD534280S1 (en) 2005-05-04 2006-12-26 Abbott Laboratories Reagent carrier for use in an automated analyzer
JP4875066B2 (ja) 2005-05-06 2012-02-15 カリパー・ライフ・サイエンシズ・インク． 除去された周囲を有するマイクロタイタープレート
WO2006122312A2 (en) 2005-05-11 2006-11-16 The Trustees Of The University Of Pennsylvania Methods of testing using a microfluidic cassette
JP4995197B2 (ja) 2005-07-01 2012-08-08 ハネウェル・インターナショナル・インコーポレーテッド ３ｄ流体力学的集束を有する成形カートリッジ
US7527763B2 (en) 2005-07-05 2009-05-05 3M Innovative Properties Company Valve control system for a rotating multiplex fluorescence detection device
US20080226502A1 (en) 2005-07-07 2008-09-18 Jacques Jonsmann Microfluidic Methods and Support Instruments
US20070020699A1 (en) 2005-07-19 2007-01-25 Idexx Laboratories, Inc. Lateral flow assay and device using magnetic particles
JP4581128B2 (ja) 2005-07-20 2010-11-17 独立行政法人産業技術総合研究所 酵素免疫測定方法及びそのための酵素免疫センサ
US20070020764A1 (en) 2005-07-20 2007-01-25 Miller Kerry L Method for processing chemistry and coagulation test samples in a laboratory workcell
EP2660482B1 (en) 2005-08-22 2019-08-07 Life Technologies Corporation Vorrichtung, System und Verfahren unter Verwendung von nichtmischbaren Flüssigkeiten mit unterschiedlichen Volumen
JP2007074960A (ja) 2005-09-13 2007-03-29 Shimadzu Corp 遺伝子増幅法
USD549827S1 (en) 2005-09-16 2007-08-28 Horiba, Ltd. Blood analyzer
EP1929269A2 (en) 2005-09-30 2008-06-11 Caliper Life Sciences, Inc. Microfluidic device for purifying a biological component using magnetic beads
JP4827483B2 (ja) 2005-10-04 2011-11-30 キヤノン株式会社 核酸試料処理装置
JP4630786B2 (ja) 2005-10-04 2011-02-09 キヤノン株式会社 生化学処理装置、ｄｎａ増幅精製装置、該装置を含むｄｎａ検査装置
JP4656646B2 (ja) 2005-10-04 2011-03-23 キヤノン株式会社 表示方法と表示装置、該表示装置を備えた全自動検査装置
US20070199821A1 (en) 2005-10-05 2007-08-30 Chow Andrea W Automated two-dimensional gel electrophoresis
US7727371B2 (en) 2005-10-07 2010-06-01 Caliper Life Sciences, Inc. Electrode apparatus for use with a microfluidic device
EP1945815A4 (en) 2005-10-11 2009-02-18 Handylab Inc DEVICE FOR PREPARING SAMPLE OF POLYNUCLEOTIDES
DE102005049920A1 (de) 2005-10-17 2007-04-19 Manz Automation Ag Roboteranordnung
USD537951S1 (en) 2005-10-21 2007-03-06 Sanyo Electric Co., Ltd. Gene amplification apparatus
USD556914S1 (en) 2005-10-21 2007-12-04 Sanyo Electric Co., Ltd. Gene amplification apparatus
US20070092403A1 (en) 2005-10-21 2007-04-26 Alan Wirbisky Compact apparatus, compositions and methods for purifying nucleic acids
DE102005051850A1 (de) 2005-10-28 2007-05-03 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung zur Vervielfältigung und Detektion von Nukleinsäuren
US20070104617A1 (en) 2005-11-04 2007-05-10 Advanced Biotechnologies Limited Capped tubes
US20070116613A1 (en) 2005-11-23 2007-05-24 Donat Elsener Sample tube and system for storing and providing nucleic acid samples
WO2007064117A1 (en) 2005-11-30 2007-06-07 Electronics And Telecommunications Research Institute Affinity chromatography microdevice and method for manufacturing the same
EP1792656B1 (en) 2005-11-30 2011-11-02 F. Hoffmann-La Roche AG Integrally built, linear array of cuvettes, two dimensional array of cuvettes and system comprising two or more two-dimensional arrays of cuvettes
JP3899360B2 (ja) 2005-12-19 2007-03-28 オリンパス株式会社 Ｄｎａ増幅装置
JP5175213B2 (ja) 2005-12-22 2013-04-03 ハネウェル・インターナショナル・インコーポレーテッド 携帯用サンプル分析システム
JP2007178328A (ja) 2005-12-28 2007-07-12 Shimadzu Corp 反応容器キット及び反応容器処理装置
US8158059B2 (en) 2005-12-30 2012-04-17 Sotax Corporation Integrated dissolution processing and sample transfer system
US20070154895A1 (en) 2005-12-30 2007-07-05 Caliper Life Sciences, Inc. Multi-assay microfluidic chips
SG134186A1 (en) 2006-01-12 2007-08-29 Nanyang Polytechnic Smart nano-integrated system assembly
JP5006215B2 (ja) 2006-02-07 2012-08-22 古河電気工業株式会社 光検出装置及び測定対象読取装置
US8124033B2 (en) 2006-02-17 2012-02-28 Agency, Science, Technology and Research Apparatus for regulating the temperature of a biological and/or chemical sample and method of using the same
USD538436S1 (en) 2006-03-06 2007-03-13 Steris Inc. Reprocessor for decontaminating medical, dental and veterinary instruments and articles
WO2007106579A2 (en) 2006-03-15 2007-09-20 Micronics, Inc. Integrated nucleic acid assays
US8088616B2 (en) 2006-03-24 2012-01-03 Handylab, Inc. Heater unit for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP2001990B1 (en) 2006-03-24 2016-06-29 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
USD569526S1 (en) 2006-03-27 2008-05-20 Handylab, Inc. Molecular diagnostic instrument
USD559995S1 (en) 2006-03-27 2008-01-15 Handylab, Inc. Controller cartridge for a diagnostic instrument
EP1839756A1 (en) 2006-03-31 2007-10-03 F.Hoffmann-La Roche Ag Apparatus for separating magnetic particles from liquids containing said particles, and an array of vessels suitable for use with such an apparatus
US8021531B2 (en) 2006-04-14 2011-09-20 Caliper Life Sciences, Inc. Method for modifying the concentration of reactants in a microfluidic device
CA2680532C (en) 2006-04-18 2017-03-21 Advanced Liquid Logic, Inc. Droplet-based pyrosequencing
USD554070S1 (en) 2006-05-03 2007-10-30 Data I/O Corporation Processing apparatus
USD554069S1 (en) 2006-05-03 2007-10-30 Data I/O Corporation Processing apparatus
CN101522909A (zh) 2006-05-17 2009-09-02 加利福尼亚技术学院 热循环系统
US8232091B2 (en) 2006-05-17 2012-07-31 California Institute Of Technology Thermal cycling system
US8815162B2 (en) 2006-06-08 2014-08-26 Koninklijke Philips N.V. Microelectronic sensor device for DNA detection
US7629124B2 (en) 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US7858366B2 (en) 2006-08-24 2010-12-28 Microfluidic Systems, Inc Integrated airborne substance collection and detection system
US7705739B2 (en) 2006-08-24 2010-04-27 Microfluidic Systems, Inc. Integrated airborne substance collection and detection system
US7633606B2 (en) 2006-08-24 2009-12-15 Microfluidic Systems, Inc. Integrated airborne substance collection and detection system
US9278321B2 (en) * 2006-09-06 2016-03-08 Canon U.S. Life Sciences, Inc. Chip and cartridge design configuration for performing micro-fluidic assays
US8246919B2 (en) 2006-09-21 2012-08-21 Abbott Laboratories Specimen sample rack
WO2008147382A1 (en) 2006-09-27 2008-12-04 Micronics, Inc. Integrated microfluidic assay devices and methods
US20080095673A1 (en) 2006-10-20 2008-04-24 Lin Xu Microplate with fewer peripheral artifacts
US8765076B2 (en) * 2006-11-14 2014-07-01 Handylab, Inc. Microfluidic valve and method of making same
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
KR101422467B1 (ko) 2007-02-08 2014-07-24 삼성전자주식회사 미세유체 칩 내의 형광을 검출하기 위한 시스템 및 형광검출 방법
US7985375B2 (en) 2007-04-06 2011-07-26 Qiagen Gaithersburg, Inc. Sample preparation system and method for processing clinical specimens
JP2008267950A (ja) 2007-04-19 2008-11-06 Enplas Corp 流体取扱装置
US20080257882A1 (en) 2007-04-20 2008-10-23 Bioinnovations Oy Vessel for accurate analysis
US20100129878A1 (en) * 2007-04-25 2010-05-27 Parthasarathy Ranjani V Methods for nucleic acid amplification
WO2008149282A2 (en) 2007-06-06 2008-12-11 Koninklijke Philips Electronics N. V. Microfluidic device and method of operating a microfluidic device
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
ES2648798T3 (es) 2007-07-13 2018-01-08 Handylab, Inc. Materiales de captura de polinucleótidos y métodos de utilización de los mismos
GB0719193D0 (en) 2007-10-02 2007-11-07 Advanced Biotech Ltd A Vessel
WO2009049171A2 (en) 2007-10-10 2009-04-16 Pocared Diagnostics Ltd. System for conducting the identification of bacteria in urine
USD632799S1 (en) 2008-05-15 2011-02-15 The Automation Partnership Cell dispenser
US8647593B2 (en) 2008-06-09 2014-02-11 Qiagen Gaithersburg, Inc. Magnetic microplate assembly
USD596312S1 (en) 2008-06-13 2009-07-14 Csp Technologies, Inc. Vial rack
USD595423S1 (en) 2008-06-30 2009-06-30 Ge Healthcare Bio-Sciences Ab Magnetic rack
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
US20100009351A1 (en) * 2008-07-11 2010-01-14 Handylab, Inc. Polynucleotide Capture Materials, and Method of Using Same
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
USD598566S1 (en) 2008-08-12 2009-08-18 Diagenode Societe Anonyme Medical instrument for laboratory use
DK2331954T3 (da) 2008-08-27 2020-04-06 Life Technologies Corp Apparat og fremgangsmåde til processering af biologiske prøver
KR20120012468A (ko) 2009-04-15 2012-02-10 비오까르띠 에스아 ｒｔＰＣＲ 반응을 모니터링하기 위한 광학 검출 시스템
USD599234S1 (en) 2009-04-28 2009-09-01 Shiseido Co., Ltd. Auto-sampler for chromatograph
USD638953S1 (en) 2009-05-12 2011-05-31 Invitrogen Dynal As Laboratory apparatus
GB2473868A (en) 2009-09-28 2011-03-30 Invitrogen Dynal As Apparatus and method of automated processing of biological samples
US8329117B2 (en) 2009-05-14 2012-12-11 Canon U.S. Life Sciences, Inc. Microfluidic chip features for optical and thermal isolation
WO2010130310A1 (en) 2009-05-15 2010-11-18 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Pipette head with filter and flushing means
EP2263802A1 (en) 2009-05-25 2010-12-22 F. Hoffmann-La Roche AG System and method for dispensing fluids
US9335339B2 (en) 2009-06-04 2016-05-10 Universal Bio Research Co., Ltd. Specimen testing device and method thereof
USD628305S1 (en) 2009-07-14 2010-11-30 Medical Research Council Sitting-drop microwell plate for crystalization
EP2454381A1 (en) 2009-07-16 2012-05-23 Abbott Laboratories A method for amplification of nucleic acids
JP4857373B2 (ja) * 2009-09-09 2012-01-18 国立大学法人東京農工大学 デンドリマー被覆磁気微粒子並びにその製造方法及び用途
US8658417B2 (en) 2009-09-15 2014-02-25 Qiagen Gaithersburg, Inc. Multiple-input analytical system
US20110097493A1 (en) 2009-10-27 2011-04-28 Kerr Roger S Fluid distribution manifold including non-parallel non-perpendicular slots
US20130029343A1 (en) 2010-02-22 2013-01-31 4Titude Ltd. Multiwell strips
EP2372367B1 (en) 2010-04-01 2016-05-11 F. Hoffmann-La Roche AG A computer-implemented method for operating an automated sample workcell
US20110300033A1 (en) 2010-06-07 2011-12-08 Chemence Medical, Inc. Pipette Holder and Applicator Apparatus
EP2752671A3 (en) 2010-07-23 2016-08-24 Beckman Coulter, Inc. System or method of including analytical units
JPWO2012036296A1 (ja) 2010-09-17 2014-02-03 ユニバーサル・バイオ・リサーチ株式会社 カートリッジおよび自動分析装置
CN106190806B (zh) 2011-04-15 2018-11-06 贝克顿·迪金森公司 扫描实时微流体热循环仪和用于同步的热循环和扫描光学检测的方法
JP6080397B2 (ja) 2011-07-05 2017-02-15 キヤノン株式会社 圧電材料、圧電素子、積層圧電素子、液体吐出ヘッド、液体吐出装置、超音波モータ、光学機器、圧電音響部品および電子機器
CN106996984B (zh) 2011-09-30 2018-11-27 贝克顿·迪金森公司 组合试剂条
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
EP2766711B1 (en) 2011-10-14 2017-12-13 Becton, Dickinson and Company Square wave thermal cycling
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
EP2809774B1 (en) 2012-02-02 2018-10-03 Corning Incorporated Synthetic attachment medium for cell culture
CA2863637C (en) 2012-02-03 2021-10-26 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
US9339812B2 (en) * 2012-02-13 2016-05-17 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
CN104620112B (zh) 2012-04-13 2017-11-28 贝克顿·迪金森公司 使用先前检验的残余材料反射检验样本
USD686749S1 (en) 2012-04-20 2013-07-23 Stratec Biomedical Ag Rack for holding sheaths
USD702854S1 (en) 2012-05-18 2014-04-15 Yoko Nakahana Micro tube array container
USD687567S1 (en) 2012-10-22 2013-08-06 Qiagen Gaithersburg, Inc. Tube strip for automated processing systems
EP2912174B1 (en) * 2012-10-25 2019-06-19 Neumodx Molecular, Inc. Method and materials for isolation of nucleic acid materials
USD710024S1 (en) 2013-03-14 2014-07-29 Bio-Rad Laboratories, Inc. Microplate
BR112015022459B1 (pt) 2013-03-15 2021-10-19 Becton, Dickinson And Company Sistema de tubo de processamento e de bandeja transportadora
EP4129482A1 (en) 2013-03-16 2023-02-08 Leslie Don ROBERTS Self-contained modular analytical cartridge and programmable reagent delivery system
WO2015021228A1 (en) 2013-08-08 2015-02-12 Illumina, Inc. Fluidic system for reagent delivery to a flow cell
CH709054A1 (de) 2013-12-20 2015-06-30 Tecan Trading Ag Abstandhalter für übereinandergestapelte Pipettenspitzen-Träger.
USD729404S1 (en) 2014-06-02 2015-05-12 Seahorse Bioscience Carrier
US10822661B2 (en) 2014-09-04 2020-11-03 Miodx Method of multivariate molecule analysis
USD787008S1 (en) 2015-06-29 2017-05-16 David Hale Shooting target
US10168347B2 (en) 2016-05-23 2019-01-01 Becton, Dickinson And Company Liquid dispenser with manifold mount for modular independently-actuated pipette channels

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