RU2015129061A - Регулярные элементы растений и их применение - Google Patents
Регулярные элементы растений и их применение Download PDFInfo
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- RU2015129061A RU2015129061A RU2015129061A RU2015129061A RU2015129061A RU 2015129061 A RU2015129061 A RU 2015129061A RU 2015129061 A RU2015129061 A RU 2015129061A RU 2015129061 A RU2015129061 A RU 2015129061A RU 2015129061 A RU2015129061 A RU 2015129061A
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- dna molecule
- seq
- dna sequence
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- plant
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- 102000053602 DNA Human genes 0.000 claims 18
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 16
- 230000009261 transgenic effect Effects 0.000 claims 12
- 108020004511 Recombinant DNA Proteins 0.000 claims 11
- 108020004414 DNA Proteins 0.000 claims 7
- 239000012634 fragment Substances 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 230000009418 agronomic effect Effects 0.000 claims 3
- 238000000034 method Methods 0.000 claims 2
- 230000001105 regulatory effect Effects 0.000 claims 2
- 241000607479 Yersinia pestis Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 claims 1
- 239000004009 herbicide Substances 0.000 claims 1
- 230000008929 regeneration Effects 0.000 claims 1
- 238000011069 regeneration method Methods 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Physiology (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Claims (29)
1. Рекомбинантная молекула ДНК, содержащая последовательность ДНК, выбранную из группы, состоящей из:
а) последовательности ДНК с по меньшей мере 85 % идентичности последовательности к любой из SEQ ID №№: 1-98 и 168-171;
б) последовательности ДНК, содержащей любую из SEQ ID №№: 1-98 и 168-171; и
в) фрагмента любой из SEQ ID №№: 1-98 и 168-171, причем фрагмент обладает ген-регуляторной активностью;
причем указанная последовательность ДНК функционально связана с гетерологической транскрибируемой молекулой ДНК.
2. Рекомбинантная молекула ДНК по п. 1, отличающаяся тем, что указанная последовательность ДНК имеет по меньшей мере около 90 % идентичности последовательности к последовательности ДНК любой из SEQ ID №№: 1-98 и 168-171.
3. Рекомбинантная молекула ДНК по п. 1, отличающаяся тем, что указанная последовательность ДНК имеет по меньшей мере 95 % идентичности последовательности к последовательности ДНК любой из SEQ ID №№: 1-98 и 168-171.
4. Молекула ДНК по п. 1, отличающаяся тем, что гетерологическая транскрибируемая молекула ДНК представляет собой ген, представляющий агрономический интерес.
5. Рекомбинантная молекула ДНК по п. 4, отличающаяся тем, что ген, представляющий агрономический интерес, обуславливает у растений толерантность к гербицидам.
6. Рекомбинантная молекула ДНК по п. 4, отличающаяся тем, что ген, представляющий агрономический интерес, обуславливает у растений устойчивость к вредителям.
7. Генетическая конструкция, содержащая молекулу рекомбинантной ДНК по п. 1.
8. Клетка трансгенного растения, содержащая рекомбинантную молекулу ДНК, содержащую последовательность ДНК, выбранную из группы, состоящей из:
а) последовательности ДНК с по меньшей мере 85 % идентичности последовательности к любой из SEQ ID №№: 1-98 и 168-171;
б) последовательности ДНК, содержащей любую из SEQ ID №№: 1-98 и 168-171; и
в) фрагмента любой из SEQ ID №№: 1-98 и 168-171, причем данный фрагмент обладает ген-регуляторной активностью;
причем указанная последовательность ДНК функционально связана с гетерологической транскрибируемой молекулой ДНК.
9. Клетка трансгенного растения по п. 8, отличающаяся тем, что указанная клетка трансгенного растения представляет собой клетку однодольного растения.
10. Клетка трансгенного растения по п. 8, отличающаяся тем, что указанная клетка трансгенного растения представляет собой клетку двудольного растения.
11. Трансгенное растение, или его часть, содержащее рекомбинантную молекулу ДНК, содержащую последовательность ДНК, выбранную из группы, состоящей из:
а) последовательности ДНК с по меньшей мере 85 % идентичности последовательности к любой из SEQ ID №№: 1-98 и 168-171;
б) последовательности ДНК, содержащей любую из SEQ ID №№: 1-98 и 168-171; и
в) фрагмента любой из SEQ ID №№: 1-98 и 168-171, причем данный фрагмент обладает ген-регуляторной активностью;
причем указанная последовательность ДНК функционально связана с гетерологической транскрибируемой молекулой ДНК.
12. Растение-потомок трансгенного растения по п. 11, отличающееся тем, что растение-потомок содержит указанную молекулу рекомбинантной ДНК.
13. Трансгенное семя из трансгенного растения по п. 11, отличающееся тем, что данное семя содержит указанную молекулу рекомбинантной ДНК.
14. Способ экспрессии транскрибируемой молекулы ДНК, включающий следующие этапы: получение трансгенного растения по п. 11 и культивирование указанного растения, причем транскрибируемая молекула ДНК экспрессируется.
15. Способ получения трансгенного растения, включающий следующие этапы:
а) трансформацию клетки растения рекомбинантной молекулой ДНК по п. 1 для получения трансформированной клетки растения; и
б) регенерацию трансгенного растения из трансформированной клетки растения.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261739720P | 2012-12-19 | 2012-12-19 | |
| US61/739,720 | 2012-12-19 | ||
| PCT/US2013/075813 WO2014100009A2 (en) | 2012-12-19 | 2013-12-17 | Plant regulatory elements and uses thereof |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2018135979A Division RU2799433C2 (ru) | 2012-12-19 | 2013-12-17 | Регуляторные элементы растений и их применение |
| RU2018135984A Division RU2796556C2 (ru) | 2012-12-19 | 2013-12-17 | Регуляторные элементы растений и их применение |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2015129061A true RU2015129061A (ru) | 2017-01-25 |
| RU2670887C2 RU2670887C2 (ru) | 2018-10-25 |
| RU2670887C9 RU2670887C9 (ru) | 2018-12-17 |
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| RU2015129061A RU2670887C9 (ru) | 2012-12-19 | 2013-12-17 | Регуляторные элементы растений и их применение |
Country Status (20)
| Country | Link |
|---|---|
| US (3) | US9303266B2 (ru) |
| EP (3) | EP2935586B1 (ru) |
| JP (4) | JP6457399B2 (ru) |
| KR (3) | KR102281540B1 (ru) |
| CN (3) | CN104995301B (ru) |
| AP (1) | AP2015008825A0 (ru) |
| AR (4) | AR094188A1 (ru) |
| AU (3) | AU2013362921B2 (ru) |
| BR (1) | BR112015014823A2 (ru) |
| CA (3) | CA2895184C (ru) |
| CL (3) | CL2015001750A1 (ru) |
| CR (3) | CR20200030A (ru) |
| ES (1) | ES2691941T3 (ru) |
| MX (2) | MX370509B (ru) |
| PH (3) | PH12015501434A1 (ru) |
| RU (1) | RU2670887C9 (ru) |
| UA (1) | UA118661C2 (ru) |
| UY (2) | UY35203A (ru) |
| WO (1) | WO2014100009A2 (ru) |
| ZA (1) | ZA201504306B (ru) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2895184C (en) | 2012-12-19 | 2021-11-23 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
| NZ745763A (en) * | 2016-03-11 | 2022-08-26 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
| BR112018073946A2 (pt) * | 2016-05-24 | 2019-02-26 | Monsanto Technology Llc | elementos reguladores de plantas e suas utilizações |
| JP7049919B2 (ja) | 2018-05-29 | 2022-04-07 | 日本光電工業株式会社 | 固定装置および人工呼吸器 |
| JP7107756B2 (ja) | 2018-06-05 | 2022-07-27 | ポリプラ・エボニック株式会社 | シート及びシートの製造方法 |
| CN118792304A (zh) * | 2018-08-03 | 2024-10-18 | 孟山都技术公司 | 植物调控元件及其用途 |
| JP7275533B2 (ja) * | 2018-11-02 | 2023-05-18 | 凸版印刷株式会社 | 樹脂成形体の製造方法 |
| US11970704B2 (en) * | 2020-04-28 | 2024-04-30 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
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