KR102273360B1 - 식물 조절 요소 및 그의 용도 - Google Patents
식물 조절 요소 및 그의 용도 Download PDFInfo
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Abstract
본 발명은 식물, 식물 세포, 종자 및 자손 식물에서 유전자 발현을 조절하는데 유용한 신규한 재조합 DNA 분자 및 작제물을 제공한다. 본 발명은 또한, 본 발명의 재조합 DNA 분자를 포함하는 유전자전이 식물, 식물 세포, 식물 부위, 종자 및 자손 식물을, 그들의 사용 방법과 함께 제공한다.
Description
관련 출원에 대한 참조
본 출원은, 전문이 본 명세서에 참조로 편입된, 미국 가출원 제61/739,720호(출원일: 2012년 12월 19일)의 유익을 주장한다.
서열 목록의 포함
345 킬로바이트(마이크로소프트 윈도우즈(Microsoft Windows)(등록상표)에서 측정됨)이고 2013년 12월 17일에 작성된 "MONS323WOseq.txt"로 명명된 파일에 포함된 서열 목록이 본 명세서에 전자 파일로 제출되고, 본 명세서에 참고로 편입된다.
본 발명의 분야
본 발명은, 식물 분자 생물학, 식물 유전 공학 분야, 및 식물에서 유전자 발현의 조절에 유용한 DNA 분자에 관한 것이다.
조절 요소는 작동 가능하게 연결된 전사가능 DNA 분자의 전사를 조절함으로써 유전자 활성을 조절하는 유전 요소(genetic element)이다. 이러한 요소는 프로모터, 리더, 인핸서, 인트론 및 3' 비번역 영역을 포함하고, 식물 분자 생물학 및 식물 유전 공학 분야에 유용하다.
본 발명은 식물에서 사용하기 위한 신규한 유전자 조절 요소 및 이러한 조절 요소를 포함하는 작제물(construct)을 제공한다. 본 발명은 또한 조절 요소를 포함하는 트랜스제닉, 즉, 유전자전이(transgenic) 식물 세포, 식물, 식물 부위 및 종자를 제공한다. 일 실시형태에 있어서, 본 발명은 전사 가능 DNA 분자에 작동 가능하게 연결된 본 명세서에 개시된 조절 요소를 제공한다. 소정의 실시형태에서, 전사가능 DNA 분자는 본 명세서에서 제공되는 조절 요소 서열에 관하여 이종성이다. 또, 본 명세서에서는, 조절 요소에 관하여 이종성인 전사 가능 DNA 분자에 작동 가능하게 연결된 조절 요소를 포함하는 작제물, 그리고 조절 요소를 포함하는 유전자전이 식물, 식물 세포, 식물 부위 및 종자를 비롯하여, 본 명세서에 개시된 조절 요소를 제조하는 방법 및 이용하는 방법을 제공한다.
이와 같이 해서, 일 양상에 있어서, 본 발명은 a) 서열번호 1 내지 98 및 168 내지 171 중 어느 하나에 대해서 적어도 약 85퍼센트(%)의 서열 동일성을 갖는 DNA 서열; b) 서열번호 1 내지 98 및 168 내지 171 중 어느 하나를 포함하는 DNA 서열; 및 c) 서열번호 1 내지 98 및 168 내지 171 중 어느 하나의 단편(여기서 상기 단편은 유전자-조절 활성을 지님)으로 이루어진 군으로부터 선택된 DNA 서열을 포함하는 재조합 DNA 분자를 제공하되; 여기서 상기 DNA 서열은 이종성 전사 가능 DNA 분자에 작동 가능하게 연결된다. "이종성 전사 가능(heterologous transcribable) DNA 분자"란, 전사 가능 DNA 분자가 DNA 서열에 관하여 이종성인 것을 의미한다. 구체적인 실시형태에 있어서, 재조합 DNA 분자는 서열번호 1 내지 98 및 168 내지 171 중 어느 하나의 DNA 서열에 대해서 적어도 90%, 적어도 91%, 적어도 92%, 적어도 93%, 적어도 94%, 적어도 약 95%, 적어도 96%, 적어도 97%, 적어도 98% 또는 적어도 99%의 서열 동일성을 갖는 DNA 서열을 포함한다. 특정 실시형태에 있어서, 이종성 전사 가능 DNA 분자는 식물에서 제초제 내성 또는 내충성(해충 저항성)(pest resistance)을 부여할 수 있는 유전자 등과 같은 관심대상 작물학적 유전자(gene of agronomic interest)를 포함한다. 또 다른 실시형태에 있어서, 본 발명은 본 명세서에서 제공된 바와 같은 재조합 DNA 분자를 포함하는 작제물을 제공한다.
다른 양상에 있어서, 본 명세서에서는, a) 서열번호 1 내지 98 및 168 내지 171 중 어느 하나에 대해서 적어도 약 85%의 서열 동일성을 갖는 DNA 서열; b) 서열번호 1 내지 98 및 168 내지 171 중 어느 하나를 포함하는 DNA 서열; 및 c) 서열번호 1 내지 98 및 168 내지 171 중 어느 하나의 단편(여기서 상기 단편은 유전자-조절 활성을 지님)으로 이루어진 군으로부터 선택된 DNA 서열을 포함하는 재조합 DNA 분자를 포함하는 유전자전이 식물 세포를 제공하되; 여기서 DNA 서열은 이종성 전사 가능 DNA 분자에 작동 가능하게 연결된다. 소정의 실시형태에 있어서, 유전자전이 식물 세포는 외떡잎 식물 세포이다. 다른 실시형태에 있어서, 유전자전이 식물 세포는 쌍떡잎 식물 세포이다.
또 다른 양상에 있어서, 본 명세서에서는, a) 서열번호 1 내지 98 및 168 내지 171 중 어느 하나에 대해서 적어도 약 85%의 서열 동일성을 갖는 DNA 서열; b) 서열번호 1 내지 98 및 168 내지 171 중 어느 하나를 포함하는 DNA 서열; 및 c) 서열번호 1 내지 98 및 168 내지 171 중 어느 하나의 단편(여기서 상기 단편은 유전자-조절 활성을 지님)으로 이루어진 군으로부터 선택된 DNA 서열을 포함하는 재조합 DNA 분자를 포함하는 유전자전이 식물 또는 그의 부분을 제공하되; 여기서 DNA 서열은 이종성 전사 가능 DNA 분자에 작동 가능하게 연결된다. 구체적인 실시형태에 있어서, 유전자전이 식물은 출발 유전자전이 식물에 대해서 임의의 세대의 자손 식물이고 상기 재조합 DNA 분자를 포함한다. 증식 시 이러한 유전자전이 식물을 생산하는 재조합 DNA 분자를 포함하는 유전자전이 종자가 또한 본 발명에 의해 제공된다.
또 다른 양상에 있어서, 본 발명은, 본 발명의 재조합 DNA 분자를 포함하는 유전자전이 식물을 얻고 해당 식물을 재배함으로써, 유전자전이 식물에서 관심대상 작물학적 유전자 등과 같은 전사 가능 DNA 분자를 발현하는 방법을 제공한다.
또한, 본 명세서에서는, 식물 세포를 본 발명의 재조합 DNA 분자로 형질전환(transforming)시켜 형질전환된 식물 세포를 생산하고 형질전환된 식물 세포를 재생시켜 유전자전이 식물을 생산함으로써, 유전자전이 식물을 생산하는 방법이 제공된다.
도 1: 아그로스티스 네불로사(Agrostis nebulosa)로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 1은 P-AGRne.Ubq1-1:1:5의 프로모터 변이체와 함께 조절 발현 요소군(EXP) EXP-AGRne.Ubq1:1:7(서열번호 1)에 포함된 2005 염기쌍(bp) 프로모터 P-AGRne.Ubq1-1:1:5(서열번호 2)의 정렬을 도시한다. 예를 들어, P-AGRne.Ubq1-1:1:5의 5' 단부의 결실은 EXP-AGRne.Ubq1:1:8(서열번호 5)에 포함되는 999 bp 서열인 프로모터 P-AGRne.Ubq1-1:1:4(서열번호 6)를 생성하였다. 도 1에 도시된 다른 프로모터 변이체는 EXP-AGRne.Ubq1:1:9(서열번호 7)에 포함된 762 bp 서열인 P-AGRne.Ubq1-1:1:6(서열번호 8)이다.
도 2: 아룬도 도낙스(Arundo donax)로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 2는 P-ARUdo.Ubq1-1:1:4의 프로모터 변이체와 함께 조절 발현 요소군 EXP-ARUdo.Ubq1:1:4(서열번호 9)에 포함된 4114 bp 프로모터 P-ARUdo.Ubq1-1:1:4(서열번호 10)의 정렬을 도시한다. 이 정렬에는 2012 bp 프로모터 P-ARUdo.Ubq1-1:1:5(서열번호 14); 1000 bp 프로모터 P-ARUdo.Ubq1-1:1:6(서열번호 17); 및 755 bp 프로모터 P-ARUdo.Ubq1-1:1:8(서열번호 22)이 포함된다.
도 3: 아룬도 도낙스로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 3은 P-ARUdo.Ubq2-1:1:4의 프로모터 변이체와 함께 2033 bp 프로모터 P-ARUdo.Ubq2-1:1:4(서열번호 24)의 정렬을 도시한다. 이 정렬에는 2004 bp 프로모터 P-ARUdo.Ubq2-1:1:6(서열번호 28); 1001 bp 프로모터 P-ARUdo.Ubq2-1:1:5(서열번호 31); 및 696 bp 프로모터 P-ARUdo.Ubq2-1:1:7(서열번호 33)이 포함된다.
도 4: 보우텔로우아 그라실리스(Bouteloua gracilis)로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 4는 P-BOUgr.Ubq1-1:1:2의 5' 단부의 프로모터 변이체와 함께 2371 bp 프로모터 P-BOUgr.Ubq1-1:1:2(서열번호 35)의 정렬을 도시한다. 이 정렬에는 1999 bp 프로모터 P-BOUgr.Ubq1-1:1:3(서열번호 39); 1022 bp 프로모터 P-BOUgr.Ubq1-1:1:5(서열번호 42); 및 760 bp 프로모터 P-BOUgr.Ubq1-1:1:6(서열번호 44)이 포함된다.
도 5: 보우텔로우아 그라실리스로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 5는 P-BOUgr.Ubq2-1:1:4의 프로모터 변이체와 함께 2100 bp 프로모터 요소 P-BOUgr.Ubq2-1:1:4(서열번호 46)의 정렬을 도시한다. 이 정렬에는 2043 bp 프로모터 P-BOUgr.Ubq2-1:1:7(서열번호 50); 2002 bp 프로모터 P-BOUgr.Ubq2-1:1:5(서열번호 53); 1024 bp 프로모터 P-BOUgr.Ubq2-1:1:6(서열번호 56); 및 749 bp 프로모터 P-BOUgr.Ubq2-1:1:8(서열번호 61)이 포함된다.
도 6: 미스칸투스 시네시스(Miscanthus sinesis)로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 6은 P-MISsi.Ubq1-1:1:2의 프로모터 변이체와 함께 5359 bp 프로모터 요소 P-MISsi.Ubq1-1:1:2(서열번호 63)의 정렬을 도시한다. 이 정렬에는 2423 bp 프로모터 P-MISsi.Ubq1-1:1:11(서열번호 67); 1447 bp 프로모터 P-MISsi.Ubq1-1:1:10(서열번호 71); 899 bp 프로모터 P-MISsi.Ubq1-1:1:13(서열번호 73); 691 bp 프로모터 P-MISsi.Ubq1-1:1:14(서열번호 75); 및 506 bp 프로모터 P-MISsi.Ubq1-1:1:9(서열번호 77)가 포함된다.
도 7: 쉬자키움 스코파륨(Schizachyium scoparium)으로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 7은 P-SCHsc.Ubq1-1:1:12의 프로모터 변이체와 함께 2831 bp 프로모터 요소, P-SCHsc.Ubq1-1:1:12(서열번호 79)의 정렬을 도시한다. 이 정렬에는 2033 bp 프로모터 P-SCHsc.Ubq1-1:1:11(서열번호 83); 1046 bp 프로모터 P-SCHsc.Ubq1-1:1:10(서열번호 85); 및 547 bp 프로모터 P-SCHsc.Ubq1-1:1:14(서열번호 87)가 포함된다.
도 8: 소가스트룸 누탄스(Sorghastrum nutans)로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 8은 P-SORnu.Ubq1-1:1:4의 프로모터 변이체와 함께 2218 bp 프로모터 요소 P-SORnu.Ubq1-1:1:4(서열번호 89)의 정렬을 도시한다. 이 정렬에는 1964 bp 프로모터 P-SORnu.Ubq1-1:1:5(서열번호 93); 1023 bp 프로모터 P-SORnu.Ubq1-1:1:6(서열번호 96); 및 724 bp 프로모터 P-SORnu.Ubq1-1:1:7(서열번호 98)이 포함된다.
도 9: 본 발명의 발현 카세트 구성을 도시한다.
서열의 간단한 설명
서열번호 1, 5, 7, 9, 13, 16, 18, 19, 21, 23, 27, 30, 32, 34, 38, 41, 43, 45, 49, 52, 55, 58, 60, 62, 66, 70, 72, 74, 76, 78, 82, 84, 86, 88, 92, 95, 97, 99, 103, 106, 108, 110, 114, 116, 118, 120, 122, 126, 128, 132, 134, 138, 140, 144, 148, 150 및 168은 인트론 서열에 5' 작동 가능하게 연결된 리더 서열에 5' 작동 가능하게 연결된 프로모터 서열을 포함하는 조절 발현 요소군(EXP)의 DNA 서열이다.
서열번호 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98, 100, 104, 107, 109, 111, 117, 119, 121, 123, 129, 135, 141, 145, 151 및 169는 프로모터 서열이다.
서열번호 3, 11, 25, 36, 47, 64, 68, 80, 90, 101, 112, 124, 130, 136, 142, 146, 152 및 170은 리더 서열이다.
서열번호 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94, 102, 105, 113, 115, 125, 127, 131, 133, 137, 139, 143, 147, 149, 153 및 171은 인트론 서열이다.
도 2: 아룬도 도낙스(Arundo donax)로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 2는 P-ARUdo.Ubq1-1:1:4의 프로모터 변이체와 함께 조절 발현 요소군 EXP-ARUdo.Ubq1:1:4(서열번호 9)에 포함된 4114 bp 프로모터 P-ARUdo.Ubq1-1:1:4(서열번호 10)의 정렬을 도시한다. 이 정렬에는 2012 bp 프로모터 P-ARUdo.Ubq1-1:1:5(서열번호 14); 1000 bp 프로모터 P-ARUdo.Ubq1-1:1:6(서열번호 17); 및 755 bp 프로모터 P-ARUdo.Ubq1-1:1:8(서열번호 22)이 포함된다.
도 3: 아룬도 도낙스로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 3은 P-ARUdo.Ubq2-1:1:4의 프로모터 변이체와 함께 2033 bp 프로모터 P-ARUdo.Ubq2-1:1:4(서열번호 24)의 정렬을 도시한다. 이 정렬에는 2004 bp 프로모터 P-ARUdo.Ubq2-1:1:6(서열번호 28); 1001 bp 프로모터 P-ARUdo.Ubq2-1:1:5(서열번호 31); 및 696 bp 프로모터 P-ARUdo.Ubq2-1:1:7(서열번호 33)이 포함된다.
도 4: 보우텔로우아 그라실리스(Bouteloua gracilis)로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 4는 P-BOUgr.Ubq1-1:1:2의 5' 단부의 프로모터 변이체와 함께 2371 bp 프로모터 P-BOUgr.Ubq1-1:1:2(서열번호 35)의 정렬을 도시한다. 이 정렬에는 1999 bp 프로모터 P-BOUgr.Ubq1-1:1:3(서열번호 39); 1022 bp 프로모터 P-BOUgr.Ubq1-1:1:5(서열번호 42); 및 760 bp 프로모터 P-BOUgr.Ubq1-1:1:6(서열번호 44)이 포함된다.
도 5: 보우텔로우아 그라실리스로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 5는 P-BOUgr.Ubq2-1:1:4의 프로모터 변이체와 함께 2100 bp 프로모터 요소 P-BOUgr.Ubq2-1:1:4(서열번호 46)의 정렬을 도시한다. 이 정렬에는 2043 bp 프로모터 P-BOUgr.Ubq2-1:1:7(서열번호 50); 2002 bp 프로모터 P-BOUgr.Ubq2-1:1:5(서열번호 53); 1024 bp 프로모터 P-BOUgr.Ubq2-1:1:6(서열번호 56); 및 749 bp 프로모터 P-BOUgr.Ubq2-1:1:8(서열번호 61)이 포함된다.
도 6: 미스칸투스 시네시스(Miscanthus sinesis)로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 6은 P-MISsi.Ubq1-1:1:2의 프로모터 변이체와 함께 5359 bp 프로모터 요소 P-MISsi.Ubq1-1:1:2(서열번호 63)의 정렬을 도시한다. 이 정렬에는 2423 bp 프로모터 P-MISsi.Ubq1-1:1:11(서열번호 67); 1447 bp 프로모터 P-MISsi.Ubq1-1:1:10(서열번호 71); 899 bp 프로모터 P-MISsi.Ubq1-1:1:13(서열번호 73); 691 bp 프로모터 P-MISsi.Ubq1-1:1:14(서열번호 75); 및 506 bp 프로모터 P-MISsi.Ubq1-1:1:9(서열번호 77)가 포함된다.
도 7: 쉬자키움 스코파륨(Schizachyium scoparium)으로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 7은 P-SCHsc.Ubq1-1:1:12의 프로모터 변이체와 함께 2831 bp 프로모터 요소, P-SCHsc.Ubq1-1:1:12(서열번호 79)의 정렬을 도시한다. 이 정렬에는 2033 bp 프로모터 P-SCHsc.Ubq1-1:1:11(서열번호 83); 1046 bp 프로모터 P-SCHsc.Ubq1-1:1:10(서열번호 85); 및 547 bp 프로모터 P-SCHsc.Ubq1-1:1:14(서열번호 87)가 포함된다.
도 8: 소가스트룸 누탄스(Sorghastrum nutans)로부터의 프로모터 요소에 대응하는 각종 크기의 다수의 프로모터 변이체의 정렬을 도시한다. 특히, 도 8은 P-SORnu.Ubq1-1:1:4의 프로모터 변이체와 함께 2218 bp 프로모터 요소 P-SORnu.Ubq1-1:1:4(서열번호 89)의 정렬을 도시한다. 이 정렬에는 1964 bp 프로모터 P-SORnu.Ubq1-1:1:5(서열번호 93); 1023 bp 프로모터 P-SORnu.Ubq1-1:1:6(서열번호 96); 및 724 bp 프로모터 P-SORnu.Ubq1-1:1:7(서열번호 98)이 포함된다.
도 9: 본 발명의 발현 카세트 구성을 도시한다.
서열의 간단한 설명
서열번호 1, 5, 7, 9, 13, 16, 18, 19, 21, 23, 27, 30, 32, 34, 38, 41, 43, 45, 49, 52, 55, 58, 60, 62, 66, 70, 72, 74, 76, 78, 82, 84, 86, 88, 92, 95, 97, 99, 103, 106, 108, 110, 114, 116, 118, 120, 122, 126, 128, 132, 134, 138, 140, 144, 148, 150 및 168은 인트론 서열에 5' 작동 가능하게 연결된 리더 서열에 5' 작동 가능하게 연결된 프로모터 서열을 포함하는 조절 발현 요소군(EXP)의 DNA 서열이다.
서열번호 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98, 100, 104, 107, 109, 111, 117, 119, 121, 123, 129, 135, 141, 145, 151 및 169는 프로모터 서열이다.
서열번호 3, 11, 25, 36, 47, 64, 68, 80, 90, 101, 112, 124, 130, 136, 142, 146, 152 및 170은 리더 서열이다.
서열번호 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94, 102, 105, 113, 115, 125, 127, 131, 133, 137, 139, 143, 147, 149, 153 및 171은 인트론 서열이다.
본 발명은 식물에서 유전자-조절 활성을 지니는 DNA 분자를 제공한다. 이들 DNA 분자의 뉴클레오타이드 서열은 서열번호 1 내지 98 및 168 내지 171로서 제공된다. 이들 DNA 분자는, 예를 들어, 식물 조직에서 작동 가능하게 연결된 전사 가능 DNA 분자의 발현에 영향을 미칠 수 있고, 따라서 유전자전이 식물에서 작동 가능하게 연결된 트랜스젠, 즉, 전이유전자(transgene)의 유전자 발현을 조절할 수 있다. 본 발명은 또한 이것을 변형, 생산 및 이용하는 방법들을 제공한다. 본 발명은 또한 본 발명의 재조합 DNA 분자를 함유하는 유전자전이 식물 세포, 식물, 식물 부위 및 종자를 포함하는 조성물과, 이를 제조 및 이용하는 방법들을 제공한다.
이하의 정의들 및 방법들은 본 발명을 보다 잘 규정하고 본 발명의 실행 시 당업계에서 통상의 기술을 가진 자에게 지침이 되도록 제공된다. 달리 언급되지 않는 한, 용어는 관련 기술 분야에서 통상의 기술을 가진 자가 통상적인 용법에 따라 이해할 것이다.
DNA 분자
본 명세서에서 사용되는 바와 같이, 용어 "DNA" 또는 "DNA 분자"는, 세포 혹은 합성 기원의 이중가닥 DNA 분자, 즉, 데옥시리보뉴클레오타이드 염기의 중합체를 지칭한다. 본 명세서에서 사용되는 바와 같이, 용어 "DNA 서열"은 DNA 분자의 뉴클레오타이드 서열을 지칭한다. 본 명세서에서 사용된 명명법은 WIPO 표준 ST.25 (1998)의 표들, 부록 2, 표 1 및 3에 제시되고 미국 연방 규정집(United States Code of Federal Regulations) § 1.822의 표제 37에 의한 것에 대응한다.
본 명세서에서 사용되는 바와 같이, "재조합 DNA 분자"는 인간을 개재하는 일 없이 자연적으로 함께 발생되지 않는 DNA 분자의 조합을 포함하는 DNA 분자이다. 예를 들어, 재조합 DNA 분자는 서로에 관하여 이종성인 적어도 2개의 DNA 분자, 자연에 존재하는 DNA 서열에서 벗어난 DNA 서열을 포함하는 DNA 분자, 또는 유전자 형질전환에 의해 숙주 세포의 DNA 내로 혼입되는 DNA 분자를 포함하는 DNA 분자일 수 있다.
본 명세서에서 사용되는 바와 같이, 용어 "서열 동일성"은 2개의 최적으로 정렬된 DNA 서열이 동일한 정도를 의미한다. 최적 서열 정렬은 2개의 DNA 서열, 예컨대, 참조 서열(reference sequence)과 다른 DNA 서열을 상호 정렬시켜, 적절한 내부 뉴클레오타이드 삽입, 결실 또는 간극을 지니는 서열 정렬에서 뉴클레오타이드 정합의 수를 최대화함으로써 작성된다. 본 명세서에서 사용되는 바와 같이, 용어 "참조 서열"이란 서열번호 1 내지 98 및 168 내지 171로서 제공되는 DNA 서열을 의미한다.
본 명세서에서 사용되는 바와 같이, 용어 "서열 동일성 %" 또는 "% 동일성" 또는 "동일성 %"는 동일성 분율×100이다. 참조 서열과 최적으로 정렬된 DNA 서열에 대한 "동일성 분율"은 최적 정렬 내의 뉴클레오타이드 정합의 수를 참조 서열 내의 뉴클레오타이드의 총수, 예컨대, 전체 참조 서열의 전체 길이 내의 뉴클레오타이드의 총수로 나눈 값이다. 따라서, 본 발명의 일 실시형태는, 서열번호 1 내지 98 및 168 내지 171로서 본 명세서에서 제공되는 참조 서열에 최적으로 정렬될 때, 참조 서열에 대해 적어도 약 85% 동일성, 적어도 약 86% 동일성, 적어도 약 87% 동일성, 적어도 약 88% 동일성, 적어도 약 89% 동일성, 적어도 약 90% 동일성, 적어도 약 91% 동일성, 적어도 약 92% 동일성, 적어도 약 93% 동일성, 적어도 약 94% 동일성, 적어도 약 95% 동일성, 적어도 약 96% 동일성, 적어도 약 97% 동일성, 적어도 약 98% 동일성, 적어도 약 99% 동일성 또는 적어도 약 100% 동일성을 갖는 DNA 서열을 포함하는 DNA 분자를 제공한다.
조절 요소
프로모터, 리더, 인핸서, 인트론 및 전사 종결 영역(또는 3' UTR) 등과 같은 조절 요소는, 살아있는 세포에서 유전자들의 전체 발현에서 통합적인 역할을 한다. 용어 "조절 요소"는, 본 명세서에서 사용되는 바와 같이, 유전자-조절 활성을 지니는 DNA 분자를 의미한다. 용어 "유전자-조절 활성"은, 본 명세서에서 사용되는 바와 같이, 예를 들어, 작동 가능하게 연결된 전사 가능 DNA 분자의 전사 및/또는 번역에 영향을 미침으로써 작동 가능하게 연결된 전사 가능 DNA 분자의 발현에 영향을 미치는 능력을 의미한다. 식물에서 기능하는 프로모터, 리더, 인핸서 및 인트론 등과 같은 조절 요소는 따라서 유전 공학을 통해 식물 표현형을 변형시키는데 유용하다.
본 명세서에서 사용되는 바와 같이, "조절 발현 요소군" 또는 "EXP" 서열은 작동 가능하게 연결된 발현 요소, 예컨대, 인핸서, 프로모터, 리더 및 인트론으로 이루어진 군을 의미할 수 있다. 따라서, 조절 발현 요소군은, 예를 들어, 인트론 서열에 5' 작동 가능하게 연결된 리더 서열에 5' 작동 가능하게 연결된 프로모터를 포함할 수 있다.
조절 요소는, 그의 유전자 발현 패턴, 예컨대, 구성적 발현 또는 그의 일시적, 공간적, 발생적, 조직, 환경적, 생리학적, 병리학적, 세포 주기 및/또는 화학적 반응 발현, 및 이들의 임의의 조합 등과 같은 긍정적 및/또는 부정적 효과에 의해서뿐만 아니라, 정량적 또는 정성적 표시에 의해서 특성규명될 수 있다. 본 명세서에서 사용되는 바와 같이, "유전자 발현 패턴"은 작동 가능하게 연결된 DNA 분자의 전사된 RNA 분자로의 임의의 전사 패턴이다. 전사된 RNA 분자는 번역되어 단백질 분자를 생성하거나 또는 안티센스 혹은 기타 조절 RNA 분자, 예컨대, 이중가닥 RNA(dsRNA), 운반 RNA(tRNA), 리보솜 RNA(rRNA), 마이크로RNA(miRNA) 등을 생성할 수 있다.
본 명세서에서 사용되는 바와 같이, 용어 "단백질 발현"은 전사된 RNA 분자의 단백질 분자로의 임의의 번역 패턴이다. 단백질 발현은 그의 일시적, 공간적, 발생적 또는 형태학적 질에 의해서뿐만 아니라 정량적 또는 정성적 표시에 의해서 특성규명될 수 있다.
프로모터는 작동 가능하게 연결된 전사 가능 DNA 분자의 발현을 조절하기 위한 조절 요소로서 유용하다. 본 명세서에서 사용되는 바와 같이, 용어 "프로모터"는, 일반적으로 전사를 개시시키기 위하여 RNA 중합효소 II 및 기타 단백질, 예컨대, 트랜스-작용성 잔사 인자의 인식 및 결합에 관련되는 DNA 분자를 의미한다. 프로모터는 유전자의 5' 비번역 영역(5' UTR)으로부터 유래될 수 있다. 대안적으로, 프로모터는 합성적으로 생성되거나 조작된 DNA 분자일 수 있다. 프로모터는 또한 키메라일 수 있다. 키메라 프로모터는 2종 이상의 이종성 DNA 분자의 융합을 통해 생성된다. 본 발명을 실행하는데 유용한 프로모터는 서열번호 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 및 169, 및 이들의 단편 또는 변이체를 포함한다. 본 발명의 구체적인 실시형태에 있어서, 본 명세서에 기재된 바와 같은 이러한 DNA 분자 및 그의 임의의 변이체 혹은 유도체는, 프로모터 활성을 포함하는 것으로 더욱 정의되고, 즉, 숙주 세포, 예컨대, 유전자전이 식물에서 프로모터로서 작용할 수 있다. 추가의 구체적인 실시형태에 있어서, 단편은 해당 단편이 유도되는 출발 프로모터 분자가 가진 프로모터 활성을 나타내는 것으로 정의될 수도 있거나, 또는 단편은 기저 전사 수준을 제공하고 전사 개시를 위해 RNA 중합효소 II 복합체의 인식 및 결합을 위한 TATA 박스 또는 등가의 DNA 서열을 포함하는 "최소 프로모터"를 포함할 수 있다.
일 실시형태에서, 본 명세서에 개시된 프로모터 서열의 단편이 제공된다. 프로모터 단편은 상기 설명한 바와 같은 프로모터 활성을 포함할 수 있고, 단독으로 또는 키메라 프로모터의 작제에서와 같이 다른 프로모터 및 프로모터 단편과 조합되어 유용할 수 있다. 구체적인 실시형태에서, 본 명세서에 개시된 프로모터 활성을 갖는 DNA 분자의 적어도 약 50, 적어도 약 75, 적어도 약 95, 적어도 약 100, 적어도 약 125, 적어도 약 150, 적어도 약 175, 적어도 약 200, 적어도 약 225, 적어도 약 250, 적어도 약 275, 적어도 약 300, 적어도 약 500, 적어도 약 600, 적어도 약 700, 적어도 약 750, 적어도 약 800, 적어도 약 900 또는 적어도 약 1000개 또는 그 초과의 연속적인 뉴클레오타이드를 포함하는 프로모터의 단편이 제공된다. 출발 프로모터 분자로부터 이러한 단편을 생성하는 방법이 또한 당업계에 공지되어 있다.
서열번호 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 및 169로서 제시된 프로모터들 중 어느 하나로부터 유도된 조성, 예컨대, 내부 또는 5' 결실은, 예를 들어, 발현에 대한 긍정적인 또는 부정적인 효과를 갖는 요소의 제거; 발현에 대한 긍정적인 또는 부정적인 효과를 갖는 요소의 복제; 및/또는 발현에 대한 조직- 또는 세포-특이적 효과를 갖는 요소의 복제 또는 제거를 비롯하여 발현을 개선하거나 변경하기 위해 당업계에 공지된 방법을 사용하여 생성될 수 있다. TATA 박스 요소 또는 그의 등가의 DNA 서열 및 하류 서열이 제거된 3' 결실을 포함하는, 서열번호 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 및 169로서 제시된 프로모터들 중 어느 하나로부터 유도된 조성이 예를 들어 인핸서 요소를 만들기 위해 사용될 수 있다. 발현에 대한 긍정적인 또는 부정적인; 조직 특이적; 세포 특이적; 또는 타이밍 특이적 (비제한적인 예로서, 일주기성 리듬 (circadian rhythm) 등) 효과를 갖는 임의의 요소를 제거하기 위해 추가의 결실이 이루어질 수 있다. 서열번호 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 및 169로서 제시된 프로모터들 중 어느 하나 및 이로부터 유도된 단편 또는 인핸서는 다른 인핸서 및 프로모터에 작동 가능하게 연결된 서열번호 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 및 169로서 제시된 임의의 프로모터 및 이로부터 유도된 단편 또는 인핸서로 이루어진 키메라 조절 요소 조성물을 제조하기 위해 사용될 수 있다.
본 발명에 따르면, 프로모터 또는 프로모터 단편은, 공지의 프로모터 요소의 존재, 즉, DNA 서열 특징, 예컨대, TATA 박스 및 기타 공지된 전사 인자 결합 부위 모티프에 대해서 분석될 수 있다. 이러한 공지된 프로모터 요소의 확인은 원래의 프로모터와 유사한 발현 패턴을 지니는 프로모터의 변이체를 설계하기 위하여 당업자에 의해 이용될 수 있다.
본 명세서에서 사용되는 바와 같이, 용어 "리더"는 유전자의 비번역 5' 영역(5' UTR)으로부터 단리되고 일반적으로 전사 개시 부위(TSS)와 단백질 암호화 서열 개시 부위 사이의 DNA 분절(segment)로서 규정된 DNA 분자를 의미한다. 대안적으로, 리더는 합성에 의해 생산되거나 조작된 DNA 요소일 수 있다. 리더는 작동 가능하게 연결된 전사가능 DNA 분자의 발현을 조정하기 위한 5' 조절 요소로서 사용될 수 있다. 리더 분자는 이종성 프로모터와 함께 또는 그의 천연 프로모터와 함께 사용될 수 있다. 따라서, 본 발명의 프로모터 분자는 그의 천연 리더에 작동 가능하게 연결될 수 있거나 또는 이종성 리더에 작동 가능하게 연결될 수 있다. 본 발명의 실시에 유용한 리더는 서열번호 3, 11, 25, 36, 47, 64, 68, 80, 90 및 170 또는 이들의 단편 또는 변이체를 포함한다. 구체적인 실시형태에서, 이러한 DNA 서열은 예를 들어 유전자전이 식물 세포를 포함하는 숙주 세포에서 리더로서 작용할 수 있는 것으로서 규정될 수 있다. 일 실시형태에서, 이러한 DNA 서열은 리더 활성을 포함하는 것으로서 이해될 수 있다.
서열번호 3, 11, 25, 36, 47, 64, 68, 80, 90 및 170으로서 제시된 리더 서열(5' UTR)은 조절 요소로 이루어질 수 있거나 또는 작동 가능하게 연결된 DNA 분자의 전사 또는 번역에 대해 효과를 가질 수 있는 2차 구조를 취할 수 있다. 서열번호 3, 11, 25, 36, 47, 64, 68, 80, 90 및 170으로서 제시된 리더 서열은 작동 가능하게 연결된 DNA 분자의 전사 또는 번역에 영향을 주는 키메라 조절 요소를 만들기 위해 본 발명에 따라 사용될 수 있다. 추가로, 서열번호 3, 11, 25, 36, 47, 64, 68, 80, 90 및 170으로서 제시된 리더 서열은 작동 가능하게 연결된 DNA 분자의 전사 또는 번역에 영향을 주는 키메라 리더 서열을 만들기 위해 사용될 수 있다.
본 명세서에서 사용되는 바와 같이, 용어 "인트론"은, 유전자의 게놈 카피로부터 단리되거나 확인될 수 있고 번역 전에 메신저 RNA(mRNA) 과정 동안 스플라이스 아웃되는 영역으로서 일반적으로 정의될 수 있는 DNA 분자를 의미한다. 대안적으로, 인트론은 합성적으로 생성되거나 조작되는 DNA 요소일 수 있다. 인트론은 작동 가능하게 연결된 유전자의 전사에 영향을 미치는 인핸서 요소를 함유할 수 있다. 인트론은 작동 가능하게 연결된 전사 가능 DNA 분자의 발현을 조절하기 위하여 조절 요소로서 사용될 수 있다. 작제물은 인트론을 포함할 수 있고, 인트론은 전사 가능 DNA 분자에 관하여 이종성일 수 있거나 그렇지 않을 수 있다. 당업계에서 인트론의 예는 벼 액틴 인트론 및 옥수수 HSP70 인트론을 포함한다.
식물에서, 몇몇 인트론의 작제물 내의 내포는 인트론이 결여된 작제물에 비해 mRNA 및 단백질 축적의 증가를 초래한다. 이 효과는 유전자 발현의 "인트론 매개 향상"(IME)이라고 명명되었다. 식물에서 발현을 자극하는 것으로 알려진 인트론은, 옥수수 유전자(예컨대, tubA1, Adh1, Sh1 및 Ubi1)에서, 벼 유전자(예컨대, tpi)에서, 그리고 페튜니아(예컨대, rbcS), 감자(예컨대, st-ls1) 및 아라비돕시스 탈리아나(Arabidopsis thaliana)(예컨대, ubq3 및 pat1)로부터의 것들과 같은 쌍떡잎 식물에서 확인되었다. 인트론의 스플라이스 부위 내의 결실 또는 돌연변이는 유전자 발현을 감소시키는 것으로 밝혀졌고, 이것은 스플라이싱이 IME를 위해 필요할 수도 있음을 나타낸다. 그러나, 스플라이싱 자체가 쌍떡잎 식물에서 특정 IME로서 필요하지 않다는 것은 아라비돕시스 탈리아나(A. thaliana)로부터의 pat1 유전자의 스플라이스 부위 내의 점 돌연변이에 의해 밝혀졌다. 하나의 식물 내의 동일한 인트론의 다수의 사용은, 단점을 나타내는 것으로 밝혀졌다. 이들 경우에, 적절한 재조합 DNA 요소의 작제를 위하여 기본적인 제어 요소를 수집할 필요가 있다.
본 발명을 실시함에 있어서 유용한 인트론은 서열번호 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 및 171을 포함한다. 서열번호 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 및 171로서 제시된 인트론들 중 어느 하나로부터 유도된 조성물은 시스 조절 요소들의 내부적 결실 혹은 복제를 포함할 수 있고; 그리고/또는 인트론/액손 스플리스 접합부를 포함하는 5' 및 3' DNA 서열의 변경은, 프로모터 + 리더 또는 키메라 프로모터 + 리더 및 암호화 서열에 작동 가능하게 연결된 경우 발현 또는 발현의 특이성을 향상시키는데 이용될 수 있다. 인트론/엑손 경계 서열을 변형할 때, 메신저 RNA의 최종 전사체로의 프로세싱 동안 원치 않는 개시 코돈이 형성될 가능성을 제거하기 위해 스플라이스 부위(GT)의 5' 단부의 바로 앞의 뉴클레오타이드 서열 AT 또는 뉴클레오타이드 A 및 각각 스플라이스 부위(AG)의 3' 단부의 바로 다음의 뉴클레오타이드 G 또는 뉴클레오타이드 서열 TG의 사용을 피하는 것이 유익할 수 있다. 따라서, 인트론의 5' 또는 3' 단부 스플라이스 접합 부위 주위의 DNA 서열은 이러한 방식으로 변형될 수 있다. 인트론 및 본 명세서에 기재된 바와 같이 그리고 당업계에 공지된 방법을 통해서 변경된 인트론 변이체는, 작동 가능하게 연결된 DNA 분자의 발현에 대한 인트론의 효과를 결정하기 위하여 작업예로 기재된 바와 같이 실험적으로 시험될 수 있다.
본 명세서에서 사용되는 바와 같이, 본 명세서에서의 용어 "3' 전사 종료 분자", "3' 비번역 영역" 또는 "3' UTR"은 mRNA 분자의 3' 부분의 비번역 영역으로의 전사 동안 사용되는 DNA 분자를 의미한다. mRNA 분자의 3' 비번역 영역은 특이적 절단 및 폴리A 테일로도 알려진 3' 폴리아데닐화에 의해 생성될 수 있다. 3' UTR은 전사가능 DNA 분자에 작동 가능하게 연결되고 이 분자의 하류에 위치될 수 있으며, 그리고 폴리아데닐화 신호 및 전사, mRNA 프로세싱, 또는 유전자 발현에 영향을 줄 수 있는 다른 조절 신호를 포함할 수 있다. 폴리A 테일은 mRNA 안정성에서 그리고 번역의 개시에서 기능하는 것으로 여겨진다. 당업계에서 3' 전사 종료 분자의 예는 노팔린 합성효소 3' 영역; 밀 hsp17 3' 영역, 완두콩 루비스코 소형 서브유닛 3' 영역, 목화 E6 3' 영역 및 코익신 3' UTR이다.
3' UTR은 전형적으로 특이적 DNA 분자의 재조합 발현을 위해 유용한 용도를 발견하였다. 약한 3' UTR은 번역 초과(read-through)를 발생할 가능성을 지니며, 이것은 이웃하는 발현 카세트에 위치하는 DNA 분자의 발현에 영향을 미칠 수 있다. 전사 종결의 적절한 제어는 하류에 위치하는 DNA 서열(예컨대, 다른 발현 카세트)로의 번역 초과를 방지할 수 있고, RNA 중합효소의 효율적인 재순환을 더욱 허용하여 유전자 발현을 개선할 수 있다. 효율적인 전사 종결(DNA로부터 RNA 중합효소 II의 방출)은 전사의 재개시를 위한 전제조건이고, 이에 따라서 전체 전사체 수준에 직접 영향을 준다. 전사 종결 후에, 성숙 mRNA는 합성 부위 및 주형으로부터 방출되어 세포질로 이송된다. 진핵 mRNA는 폴리(A)가 생체 내에서 형성됨에 따라서 축적되므로, 통상적인 방법에 의해 전사 종결 부위를 검출하는 것은 어렵다. 그러나, 생물정보학(bioinformatics) 방법에 의한 기능적이면서도 효율적인 3' UTR의 예측은, 효과적인 3' UTR의 쉬운 예측을 허용하는 보존된 DNA 서열이 존재하지 않는다는 점에서 어렵다.
실용적인 관점에서, 발현 카세트에 사용된 3' UTR은 다음 특징을 갖는 것이 전형적으로 유익하다. 3' UTR은 전이유전자의 전사를 효율적으로 및 효과적으로 종료하고, 하나의 운반 DNA(T-DNA) 상에 존재하는 다중 카세트, 또는 T-DNA가 삽입된 이웃하는 염색체 DNA의 경우에서처럼 다른 발현 카세트에 포함될 수 있는 임의의 이웃하는 DNA 서열로의 전사체의 번역 초과를 방지할 수 있어야 한다. 3' UTR은 DNA 분자의 발현을 유도하는데 사용되는 프로모터, 리더, 인핸서 및 인트론에 의해 부여된 전사 활성의 감소를 초래하지 않아야 한다. 식물 생명공학에서, 3' UTR은 종종 형질전환된 식물로부터 추출된 역전사된 RNA의 증폭 반응의 프라이밍(priming)을 위해 사용되고, (1) 일단 식물 염색체 내로 통합된 후에 발현 카세트의 전사 활성 또는 발현을 평가하고; (2) 식물 DNA 내의 삽입 카피수를 평가하고; 그리고 (3) 육종 후에 얻어지는 종자의 접합 상태(zygosity)를 평가하기 위해 사용된다. 3' UTR은 또한 삽입된 카세트의 무손상성의 특징을 결정하기 위해 형질전환된 식물로부터 추출된 DNA의 증폭 반응에 사용된다.
본 명세서에서 사용되는 바와 같이, 용어 "인핸서" 또는 "인핸서 요소"는 전체 발현 패턴의 측면을 부여하지만 통상 단독으로 작동 가능하게 연결된 DNA 서열의 전사를 유도하기에는 불충분한, 시스-요소로도 알려진 시스-작용성 전사 조절 요소를 의미한다. 프로모터와 달리, 인핸서 요소는 통상 전사 개시 부위(TSS) 또는 TATA 박스 또는 등가의 DNA 서열을 포함하지 않는다. 프로모터 또는 프로모터 단편은 작동 가능하게 연결된 DNA 서열의 전사에 영향을 주는 하나 이상의 인핸서 요소를 천연적으로 포함할 수 있다. 또한, 인핸서 요소는 프로모터에 융합되어, 유전자 발현의 전체 조정 양상을 부여하는 키메라 프로모터 시스-요소를 생성할 수 있다.
많은 프로모터 인핸서 요소는 DNA-결합 단백질에 결합하고/하거나 DNA 토폴로지(topology)에 영향을 주고, 이에 의해 DNA 주형에 대한 RNA 중합효소의 접근을 선택적으로 허용하거나 제한하거나 또는 전사 개시 부위에서 이중 나선의 선택적인 개방을 용이하게 하는 국소 입체형태를 생성하는 것으로 생각된다. 인핸서 요소는 전사를 조절하는 전사 인자에 결합하는 기능을 수행할 수 있다. 몇몇의 인핸서 요소는 하나 초과의 전사 인자에 결합하고, 전사 인자는 하나 초과의 인핸서 도메인과 상이한 친화도로 상호작용할 수 있다. 인핸서 요소는 결실 분석, 즉, 5' 단부 또는 내부로부터 프로모터까지 하나 이상의 뉴클레오타이드의 결실; DNase I 풋프린팅(footprinting), 메틸화 간섭, 전기영동 이동도-전환 검정, 결찰-매개 중합효소 연쇄 반응(PCR)에 의한 생체내 게놈 풋프린팅, 및 다른 통상적인 검정을 사용한 DNA 결합 단백질 분석; 또는 통상적인 DNA 서열 비교 방법, 예컨대, BLAST에 의한, 공지의 시스-요소 모티프 또는 인핸서 요소를 표적 서열 또는 표적 모티프로서 사용하는 DNA 서열 유사성 분석을 포함하는 많은 수법에 의해 확인할 수 있다. 인핸서 도메인의 정밀한 구조는 하나 이상의 뉴클레오타이드의 돌연변이 유발(또는 치환) 또는 다른 통상적인 방법에 의해 추가로 연구될 수 있다. 인핸서 요소들은 화학적 합성에 의해 또는 이러한 요소를 포함하는 조절 요소로부터의 단리에 의해 얻어질 수 있고, 이들은 하위서열 조작을 용이하게 하도록 유용한 제한 효소를 함유하는 추가의 측면에 위치하는 뉴클레오타이드를 사용하여 합성될 수 있다. 따라서, 작동 가능하게 연결된 전사가능 DNA 분자의 발현을 조정하기 위한, 본 명세서에 개시된 방법에 따른 인핸서 요소의 설계, 작제 및 용도가 본 발명에 의해 포함된다.
본 명세서에서 사용되는 바와 같이, 용어 "키메라"는 제1 DNA 분자를 제2 DNA 분자에 융합함으로써 생성된 단일 DNA 분자를 의미하고, 여기서 제1 또는 제2 DNA 분자의 어느 것도 정상적으로는 그 입체형태, 즉, 서로 융합된 형태로 발견되지 않을 것이다. 키메라 DNA 분자는 따라서 자연에서는 정상적으로 발견되지 않는 새로운 DNA 분자이다. 본 명세서에서 사용되는 바와 같이, 용어 "키메라 프로모터"는 이러한 DNA 분자의 조작을 통해 생성된 프로모터를 의미한다. 키메라 프로모터는 2 이상의 DNA 단편을 조합할 수 있고, 예를 들어, 프로모터의 인핸서 요소에 대한 융합일 것이다. 따라서, 작동 가능하게 연결된 전사가능 DNA 분자의 발현을 조정하기 위한 본 명세서에 개시된 방법에 따른 키메라 프로모터의 설계, 작제 및 용도가 본 발명에 포함된다.
본 명세서에서 사용되는 바와 같이, 용어 "변이체"는 제1 DNA 분자와 조성이 유사하지만 동일하지는 않은 제2 DNA 분자, 예컨대, 조절 요소를 의미하며, 여기서, 제2 DNA 분자는 제1 DNA 분자의 일반적인 기능성, 즉, 동일한 또는 유사한 발현 패턴을 예를 들어, 다소의 혹은 등가의 전사 혹은 번역 활성을 통해서 여전히 유지한다. 변이체는, 제1 DNA 분자의 단축된 또는 절두된 형태 및/또는 제1 DNA 분자의 DNA 서열의 변경된 형태, 예컨대, 상이한 제한 효소 부위 및/또는 내부 결실, 치환 및/또는 삽입을 갖는 것일 수 있다. 또한, 조절 요소 "변이체"는 세균 및 식물 세포 형질전환 동안 또는 그의 결과로서 일어나는 돌연변이로부터 발생하는 변이체를 포함한다. 본 발명에서, 서열번호 1 내지 98 및 168 내지 171로 제시되는 DNA 서열은 본래의 조절 요소의 일반적인 기능성, 즉, 동일한 또는 유사한 발현 패턴을 계속 유지하면서 본래의 조절 요소의 DNA 서열과 조성이 유사하지만 동일하지 않은 변이체를 생성하는데 사용될 수 있다. 본 발명의 이러한 변이체의 생산은 본 개시내용에 비추어 해당 분야의 통상적인 기술 범위 내이고, 본 발명의 범위 내에 포함된다.
키메라 조절 요소는, 당업계에 공지된 다양한 방법, 예컨대, 제한 효소 소화 및 결찰, 결찰 독립적 클로닝, 증폭 동안 PCR 산물의 모듈식 조립(modular assembly), 또는 조절 요소의 직접적인 화학적 합성뿐만 아니라 당업계에 공지된 다른 방법에 의해 작동 가능하게 연결될 수 있는 각종 구성 요소를 포함하도록 설계될 수 있다. 생성되는 각종 키메라 조절 요소는, 동일한 구성 요소 또는 동일한 구성 요소의 변이체를 포함할 수 있지만, 구성 부분들이 작동 가능하게 연결되도록 하는 연결 DNA 서열 또는 서열들을 포함하는 DNA 서열 또는 DNA 서열들이 상이할 수 있다. 본 발명에서, 서열번호 1 내지 98 및 168 내지 171로서 제시된 DNA 서열은 조절 요소 참조 서열을 제공할 수 있고, 여기서 참조 서열을 포함하는 구성 요소는 당업계에 공지된 방법에 의해 접합될 수 있고 세균 및 식물 세포 형질전환에서 천연적으로 발생하는 하나 이상의 뉴클레오타이드의 치환, 결실 및/또는 삽입 또는 돌연변이를 포함할 수 있다.
특정 전이유전자의 소망의 발현 양상에 대한 본 명세서에 기재된 변형, 복제 혹은 결실의 효능은 그 결과를 검증하기 위하여 안정적인 과도 식물 검정, 예컨대, 본 명세서의 작업예에 기재된 것들에서 실험적으로 시험될 수 있고, 이것은 출발 DNA 분자에서 이루어진 변형 및 그 변화 목적에 따라 다를 수 있다.
작제물
본 명세서에서 사용되는 바와 같이, 용어 "작제물"은, 임의의 공급원으로부터 유래되고 게놈 통합 또는 자가 복제가 가능하고 DNA 분자를 포함하는 임의의 재조합 DNA 분자, 예컨대, 플라스미드, 코스미드, 바이러스, 파지, 또는 선형 혹은 환형 DNA 또는 RNA 분자를 의미하고, 여기서 적어도 하나의 DNA 분자는 기능적으로 작동하는 방식으로 다른 DNA 분자에 연결된, 즉, 작동 가능하게 연결된다. 본 명세서에서 사용되는 바와 같이, 용어 "벡터"는 형질전환의 목적, 즉, 이종성 DNA 또는 RNA의 숙주 세포 내로의 도입 목적을 위해 사용될 수 있는 임의의 작제물을 의미한다. 작제물은 전형적으로 하나 이상의 발현 카세트를 포함한다. 본 명세서에서 사용되는 바와 같이, "발현 카세트"는, 하나 이상의 조절 요소, 전형적으로 적어도 프로모터 및 3' UTR에 작동 가능하게 연결된 적어도 전사 가능 DNA 분자를 포함하는 DNA 분자를 의미한다.
본 명세서에서 사용되는 바와 같이, 용어 "작동 가능하게 연결된"은 제1 DNA 분자가 제2 DNA 분자의 기능에 영향을 주도록 분자가 제1 및 제2 DNA 분자가 배열된 상태로, 제1 DNA 분자가 제2 DNA 분자에 접합된 것을 의미한다. 2개의 DNA 분자는 하나의 연속적인 DNA분자의 일부이거나 아닐 수 있고, 인접하거나 인접하지 않을 수 있다. 예를 들어, 프로모터는 프로모터가 세포에서 관심 대상인 전사가능 DNA 분자의 전사를 조정할 경우에 전사가능 DNA 분자에 작동 가능하게 연결된 것이다. 예를 들어, 리더는 DNA 서열의 전자 혹은 번역에 영향을 미칠 수 있을 경우 DNA 서열에 작동 가능하게 연결된다.
본 발명의 작제물은, 일 실시형태에서, T-DNA를 포함하는 아그로박테리움 투메파시엔스(Agrobacterium tumefaciens)로부터 단리된 Ti 플라스미드의 우측 경계(RB 또는 AGRtu.RB) 및 좌측 경계(LB 또는 AGRtu.LB) 영역을 갖고, 아그로박테리움 투메파시엔스(A. tumefaciens) 세포에 의해 제공되는 전달 분자와 함께 T-DNA의 식물 세포의 게놈 내로의 통합을 허용하는 이중 종양-유도(Ti) 플라스미드 경계 작제물로서 제공될 수 있다(예를 들어 미국 특허 제6,603,061호 참조). 또한, 작제물은 또한 세균 세포에서 복제 기능 및 항생제 선택을 제공하는 플라스미드 골격 DNA 분절, 예를 들어, 에스케리키아 콜라이(Escherichia coli) 복제 기원, 예컨대, ori322, 광범위한 숙주 범위의 복제 기원, 예컨대, oriV 또는 oriRi, 및 선택가능 마커의 암호화 영역, 예컨대, 스펙티노마이신 또는 스트렙토마이신에 대한 내성을 부여하는 Tn7 아미노글라이코사이드 아데닐트랜스퍼라제(aadA)를 암호화하는 Spec/Strp, 또는 겐타마이신(Gm, Gent) 선택가능 마커 유전자를 함유할 수 있다. 식물 형질전환을 위해, 숙주 세균 균주는 종종 아그로박테리움 투메파시엔스 ABI, C58 또는 LBA4404이지만; 식물 형질전환의 당업자에게 공지된 다른 균주가 본 발명에서 기능할 수 있다.
전사가능 DNA 분자가 단백질로서 번역 및 발현되는 기능성 mRNA 분자로 전사되는 방식으로 작제물을 조립하고 세포 내로 도입하기 위한 방법은 당업계에 공지되어 있다. 본 발명의 실시를 위하여, 작제물 및 숙주 세포의 제조 및 사용을 위한 통상적인 조성물 및 방법은 당업자에게 잘 알려져 있다. 보다 고등 식물에서 핵산의 발현에 유용한 전형적인 벡터가 당업계에 잘 알려져 있고, 아그로박테리움 투메파시엔스의 Ti 플라스미드로부터 유래된 벡터 및 pCaMVCN 이송 제어 벡터를 포함한다.
본 명세서에서 제공되는 것들 중 임의의 것을 비롯하여 다양한 조절 요소가 작제물에 포함될 수 있다. 임의의 이러한 조절 요소는 다른 조절 요소와 조합되어 제공될 수 있다. 이러한 조합은 바람직한 조절 특성을 생성하도록 설계되거나 변형될 수 있다. 일 실시형태에서, 본 발명의 작제물은 3' UTR에 작동 가능하게 연결된 전사가능 DNA 분자에 작동 가능하게 연결된 적어도 하나의 조절 요소를 포함한다.
본 발명의 작제물은 본원에서 제공되거나 당업계에 공지된 임의의 프로모터 또는 리더를 포함할 수 있다. 예를 들어, 본 발명의 프로모터는 이종성 비-번역 5' 리더, 예컨대, 열 충격 단백질 유전자로부터 유래된 것에 작동 가능하게 연결될 수 있다. 대안적으로, 본 발명의 리더는 이종성 프로모터, 예컨대, 꽃양배추 모자이크 바이러스 35S 전사체 프로모터에 작동 가능하게 연결될 수 있다.
발현 카세트는 또한 작동 가능하게 연결된 단백질을, 특히 엽록체, 백색체, 또는 다른 색소체 소기관; 미토콘드리아; 과산화소체; 액포(vacuole); 또는 세포외 위치로 세포 이하 표적화하기에 유용한 펩타이드를 암호화하는 수송 펩타이드 암호화 서열을 포함할 수 있다. 많은 엽록체-위치된 단백질은 핵 유전자로부터 전구체로서 발현되고, 엽록체 수송 펩타이드(CTP)에 의해 엽록체로 표적화된다. 이러한 단리된 엽록체 단백질의 예는 리불로스-1,5-비스포스페이트 카복실라제, 페레독신, 페레독신 옥시도리덕타제, 광 포획(lightharvesting) 복합체 단백질 I 및 단백질 II, 티오레독신 F, 에놀피루빌 시키메이트 포스페이트 합성효소(EPSPS)의 소형 서브유닛(SSU)과 결합한 것들을 포함하지만, 이들로 제한되는 것은 아니다. 엽록체 수송 펩타이드는, 예를 들어, 미국 특허 제7,193,133호에 기재되어 있다. 비-엽록소 단백질은, 비-엽록소 단백질을 암호화하는 전이유전자에 작동 가능하게 연결된 이종성 CTP의 발현에 의해 엽촉체에 표적화될 수 있는 것이 입증되었다.
전사 가능 DNA 분자
본 명세서에서 사용되는 바와 같이, 용어 "전사 가능 DNA 분자"는, RNA 분자로 전사될 수 있는 임의의 DNA 분자를 의미하고, 단백질 암호화 서열을 갖는 것들 및 유전자 억제에 유용한 서열을 갖는 RNA 분자를 생산하는 것들을 포함하지만 이들로 제한되는 것은 아니다. 이러한 유형의 DNA 분자는, 동일 식물로부터의 DNA 분자, 다른 식물로부터의 DNA 분자, 다른 유기체로부터의 DNA 분자, 또는 합성 DNA 분자, 예컨대, 유전자의 안티센스 메시지를 함유하는 DNA 분자, 또는 인공, 합성 혹은 기타 변형된 형태의 전이유전자를 암호화하는 DNA 분자를 포함할 수 있지만 이들로 제한되는 것은 아니다. 본 발명의 작제물로의 혼입을 위한 예시적인 전사 가능 DNA 분자는, 예컨대, DNA 분자가 혼입되는 종 이외의 종으로부터의 DNA 분자 또는 유전자, 또는 동일 종으로부터 유래되거나 동일 종 내에 존재하지만 전통적인 생육 기법보다는 오히려 유전 공학에 의해 수용자 세포 내로 혼입되는 유전자를 포함한다.
"전이유전자"는 적어도 숙주 세포 게놈 내의 그의 위치에 관하여 숙주 세포에 대해 이종성인 전사가능 DNA 분자 및/또는 세포의 현재 또는 임의의 이전 세대에서 숙주 세포의 게놈 내로 인공적으로 혼입된 전사가능 DNA 분자를 의미한다.
조절 요소, 예컨대, 본 발명의 프로모터는, 조절 요소에 관하여 이종성인 전사 가능 DNA 분자에 작동 가능하게 연결될 수 있다. 본 명세서에서 사용되는 바와 같이, 용어 "이종성"은 그 조합이 정상적으로는 자연에서 발견되지 않는, 2 이상의 DNA 분자의 조합을 나타낸다. 예를 들어, 2개의 DNA 분자는 상이한 종으로부터 유래될 수 있고/있거나, 2개의 DNA 분자는 상이한 유전자, 예컨대, 동일한 종으로부터의 상이한 유전자 또는 상이한 종으로부터의 동일한 유전자로부터 유래될 수 있다. 따라서, 조절 요소는 이러한 조합이 정상적으로는 자연에서 발견되지 않으면, 작동 가능하게 연결된 전사가능 DNA 분자에 관하여 이종성이고, 즉, 전사 가능 DNA 분자는 자연적으로 조절 요소에 작동 가능한 연결이 일어나지 않는다.
전사 가능 DNA 분자는 일반적으로 전사체의 발현이 요구되는 임의의 DNA 분자일 수 있다. 전사체의 그러한 발현은 얻어지는 mRNA 분자의 번역, 따라서 단백질 발현을 유발할 수 있다. 대안적으로, 예를 들어, 전사 가능 DNA 분자는 궁극적으로 특이적 유전자 또는 단백질의 발현 감소를 유발하도록 설계될 수 있다. 일 실시형태에서, 이것은 안티센스 방향으로 배향된 전사 가능 DNA 분자를 사용함으로써 달성될 수 있다. 당업자는 이러한 안티센스 기술을 사용하는 것에 친숙하다. 임의의 유전자는 이러한 방법으로 음성적으로 조절될 수 있고, 일 실시형태에 있어서, 전사 가능 DNA 분자는 dsRNA, siRNA 또는 miRNA 분자의 발현을 통한 특정 유전자의 억제를 위해 설계될 수 있다.
따라서, 본 발명의 일 실시형태는, 작제물이 유전자전이 식물 세포의 게놈에 통합될 때 전사 가능 DNA 분자의 전사를 목적으로 하는 수준으로 또는 목적으로 하는 패턴으로 조정하기 위하여 전사 가능 DNA 분자에 작동 가능하게 연결된 본 발명의 조절 요소, 예컨대, 서열번호 1 내지 98 및 168 내지 171로서 제시되는 것들을 포함하는 재조합 DNA 분자이다. 일 실시형태에서, 전사 가능 DNA 분자는 유전자의 단백질-암호화 영역을 포함하고, 다른 실시형태에 있어서, 전사 가능 DNA 분자는 유전자의 안티센스 영역을 포함한다.
관심대상 작물학적 유전자
전사 가능 DNA 분자는 관심대상 작물학적 유전자일 수 있다. 본 명세서에서 사용되는 바와 같이, 용어 "관심대상 작물학적 유전자"는, 특정 식물 조직, 세포, 또는 세포 유형에서 발현될 때, 바람직한 특징을 부여하는 전사가능 DNA 분자를 의미한다. 관심대상 작물학적 유전자의 산물은, 식물 형태, 생리학, 성장, 발달, 수율, 알갱이 조성, 영양 프로파일, 질환 내성 또는 내충성, 및/또는 환경적 또는 화학적 내성에 영향을 미치기 위하여 식물 내에서 작용할 수 있거나, 또는 식물에 공급되는 해충의 먹이에서 살충제로서 작용할 수 있다. 본 발명의 일 실시형태에 있어서, 본 발명의 조절 요소는 해당 조절 요소가 관심대상 작물학적 유전자인 전사 가능 DNA 분자에 작동 가능하게 연결되도록 작제물에 혼입된다. 이러한 작제물을 함유하는 유전자전이 식물에 있어서, 관심대상 작물학적 유전자의 발현은 유익한 작물학적 형질을 부여할 수 있다. 유익한 작물학적 형질은, 예를 들어, 제초제 내성, 곤충 방제, 변형된 수율, 질환 내성, 병원균 내성, 변형된 식물 성장 및 발달, 변형된 전분 함량, 변형된 오일 함량, 변형된 지방산 함량, 변형된 단백질 함량, 변형된 과일 숙성, 향상된 동물 및 인간 영양분, 생체 중합체 생산, 환경적 스트레스 내성, 약제학적 펩타이드, 개선된 프로세싱 품질, 개선된 풍미, 잡종 종자 생산 이용성, 개선된 섬유 생산, 및 바람직한 생체연료 생산을 포함할 수 있지만 이들로 제한되는 것은 아니다.
당업계에 공지된 관심대상 작물학적 유전자의 예는 제초제 내성(미국 특허 제6,803,501호; 제6,448,476호; 제6,248,876호; 제6,225,114호; 제6,107,549호; 제5,866,775호; 제5,804,425호; 제5,633,435호; 및 제5,463,175호), 증가된 수득량(미국 특허 제USRE38,446호; 제6,716,474호; 제6,663,906호; 제6,476,295호; 제6,441,277호; 제6,423,828호; 제6,399,330호; 제6,372,211호; 제6,235,971호; 제6,222,098호; 및 제5,716,837호), 곤충 방제(미국 특허 제6,809,078호; 제6,713,063호; 제6,686,452호; 제6,657,046호; 제6,645,497호; 제6,642,030호; 제6,639,054호; 제6,620,988호; 제6,593,293호; 제6,555,655호; 제6,538,109호; 제6,537,756호; 제6,521,442호; 제6,501,009호; 제6,468,523호; 제6,326,351호; 제6,313,378호; 제6,284,949호; 제6,281,016호; 제6,248,536호; 제6,242,241호; 제6,221,649호; 제6,177,615호; 제6,156,573호; 제6,153,814호; 제6,110,464호; 제6,093,695호; 제6,063,756호; 제6,063,597호; 제6,023,013호; 제5,959,091호; 제5,942,664호; 제5,942,658호, 제5,880,275호; 제5,763,245호; 및 제5,763,241호), 진균 질환 내성(미국 특허 제6,653,280호; 제6,573,361호; 제6,506,962호; 제6,316,407호; 제6,215,048호; 제5,516,671호; 제5,773,696호; 제6,121,436호; 제6,316,407호; 및 제6,506,962호), 바이러스 내성(미국 특허 제6,617,496호; 제6,608,241호; 제6,015,940호; 제6,013,864호; 제5,850,023호; 및 제5,304,730호), 선충 내성(미국 특허 제6,228,992호), 세균 질환 내성(미국 특허 제5,516,671호), 식물 성장 및 발달(미국 특허 제6,723,897호 및 제6,518,488호), 전분 생산(미국 특허 제6,538,181호; 제6,538,179호; 제6,538,178호; 제5,750,876호; 제6,476,295호), 변형된 오일 생산(미국 특허 제6,444,876호; 제6,426,447호; 및 제6,380,462호), 고급 오일 생산(미국 특허 제6,495,739호; 제5,608,149호; 제6,483,008호; 및 제6,476,295호), 변형된 지방산 함량(미국 특허 제6,828,475호; 제6,822,141호; 제6,770,465호; 제6,706,950호; 제6,660,849호; 제6,596,538호; 제6,589,767호; 제6,537,750호; 제6,489,461호; 및 제6,459,018호), 고단백 생산(미국 특허 제6,380,466호), 과일 숙성(미국 특허 제5,512,466호), 향상된 동물 및 인간 영양분(미국 특허 제6,723,837호; 제6,653,530호; 제6,5412,59호; 제5,985,605호; 및 제6,171,640호), 생체중합체(미국 특허 제USRE37,543호; 제6,228,623호; 및 제5,958,745호 및 제6,946,588호), 환경적 스트레스 내성(미국 특허 제6,072,103호), 약제학적 펩타이드 및 분비가능 펩타이드(미국 특허 제6,812,379호; 제6,774,283호; 제6,140,075호; 및 제6,080,560호), 개선된 프로세싱 형질(미국 특허 제6,476,295호), 개선된 소화율(미국 특허 제6,531,648호) 낮은 라피노스(미국 특허 제6,166,292호), 산업적 효소 생산(미국 특허 제5,543,576호), 개선된 풍미(미국 특허 제6,011,199호), 질소 고정(미국 특허 제5,229,114호), 잡종 종자 생산(미국 특허 제5,689,041호), 섬유 생산(미국 특허 제6,576,818호; 제6,271,443호; 제5,981,834호; 및 제5,869,720호) 및 생체연료 생산(미국 특허 제5,998,700호)을 위한 것들을 포함한다.
대안적으로, 관심대상 작물학적 유전자는, 예를 들어, 안티센스(예컨대, 미국 특허 제5,107,065호 참조); 저해성 RNA("RNAi", 예를 들어 미국 특허 출원 공개 제2006/0200878호 및 미국 특허 출원 공개 제2008/0066206호 및 미국 특허 출원 제11/974,469호에서 설명된 바와 같은 miRNA-, siRNA-, 트랜스-작용성 siRNA-, 및 페이스드(phased) sRNA-매개 메카니즘을 통한 유전자 발현의 조정 포함); 또는 동시 억제-매개 메카니즘에 의해 내인성 유전자 발현의 표적화된 조정을 유발하는 RNA 분자를 암호화함으로써 상기 언급된 식물 특징 또는 표현형에 영향을 줄 수 있다. 또한, RNA는 요구되는 내인성 mRNA 산물을 절단하도록 조작된 촉매적 RNA 분자(예를 들어 리보자임(ribozyme) 또는 리보스위치(riboswitch); 예를 들어 미국 특허 출원 공개 제2006/0200878호 참조)일 수 있다. 전사 가능 DNA 분자가 유전자 억제를 야기할 수 있는 분자로 전사되는 방식으로 작제물을 제작하고 세포 내로 도입하는 방법은 당업계에 공지되어 있다.
식물 세포 내에서 전사가능 DNA 분자의 발현은 식물 세포를 먹는 식물 해충, 예를 들어 딱정벌레(coleopteran) 해충으로부터 단리된 조성물 및 선충 해충으로부터 단리된 조성물을 억제하기 위해 사용될 수 있다. 식물 해충은 절지동물 해충, 선충 해충, 및 진균 또는 미생물 해충을 포함하지만 이들로 제한되는 것은 아니다.
선택 가능 마커
선택 가능 마커 전이유전자는 또한 본 발명의 조절 요소와 함께 이용될 수 있다. 본 명세서에서 이용되는 바와 같이, 용어 "선택 가능 마커 전이유전자"는 유전자전이 식물, 조직 또는 세포에서의 발현 혹은 그의 결실이 몇몇 방식으로 선별되거나 점수화될 수 있는 임의의 전사 가능 DNA 분자를 의미한다. 선택 가능 마커 유전자, 그리고 본 발명의 실시에 이용하기 위한 그들의 관련된 선택 및 선별 수법은 당업계에 공지되어 있고, β-글루쿠로니다제(GUS), 녹색 형광 단백질(GFP), 항생제 내성을 부여하는 단백질, 또는 제초제 내성을 부여하는 단백질을 암호화하는 전사 가능 DNA 분자를 포함하지만 이들로 제한되는 것은 아니다.
세포 형질전환
본 발명은 또한 전사 가능 DNA 분자에 작동 가능하게 연결된 하나 이상의 조절 요소를 포함하는 형질전환된 세포 및 식물을 생산하는 방법에 관한 것이다.
용어 "형질전환"은 수용자 숙주 내로의 DNA 분자의 도입을 의미한다. 본 명세서에서 사용되는 바와 같이, 용어 "숙주"는 임의의 세포, 조직, 기관을 포함하는 세균, 진균 또는 식물, 또는 세균 진균 또는 식물의 자손을 의미한다. 특히 관심있는 식물 조직 및 세포는, 원형질체, 캘러스(callus), 뿌리, 괴경, 종자, 줄기, 잎, 묘목, 배아 및 꽃가루를 포함한다.
본 명세서에서 사용되는 바와 같이, 용어 "형질전환된"은 외래 DNA 분자, 예컨대, 작제물이 도입된 세포, 조직, 기관 또는 유기체를 의미한다. 도입된 DNA 분자는 도입된 DNA 분자가 후속 자손에 유전되도록 수용자 세포, 조직, 기관 또는 유기체의 게놈 DNA 내로 통합될 수 있다. "유전자전이" 또는 "형질전환된" 세포 또는 유기체는 또한 세포 또는 유기체의 자손체 및 교배 시에 모체로서 이러한 유전자전이 유기체를 사용하는 육종 프로그램으로부터 생산되고 외래 DNA 분자의 존재에 의한 변경된 표현형을 나타내는 자손체를 포함한다. 도입된 DNA 분자는 또한 해당 도입된 DNA 분자가 후대 자손체에 의해 유전되지 않도록 수용자 세포 내로 일시적으로 도입될 수도 있다. 용어 "유전자전이"는 하나 이상의 이종성 DNA 분자를 함유하는 세균, 진균 또는 식물을 의미한다.
DNA 분자를 식물 세포 내로 도입하기 위한 많은 방법이 당업자에게 알려져 있다. 이 방법은 일반적으로 적합한 숙주 세포를 선택하는 단계, 숙주 세포를 벡터로 형질전환시키는 단계 및 형질전환된 숙주 세포를 얻는 단계를 포함한다. 본 발명의 실시에 있어서 식물 게놈에 식물 작제물을 도입함으로써 식물 세포를 형질전환시키기 위한 방법 및 재료는, 잘 알려져 있으면서도 입증된 방법들의 어느 하나를 포함할 수 있다. 적합한 방법은 특히 세균 감염(예를 들어 아그로박테리움), 이원성 BAC 벡터, DNA의 직접 전달(예를 들어, PEG-매개 형질전환, 건조/억제-매개 DNA 흡수, 전기천공, 탄화규소 섬유를 사용한 교반, 및 DNA 코팅된 입자의 가속에 의함) 등을 포함하지만 이들로 제한되는 것은 아니다.
숙주 세포는 임의의 세포 또는 유기체, 예컨대 식물 세포, 조류 세포(algal cell), 조류(algae), 진균 세포, 진균, 세균 세포 또는 곤충 세포일 수 있다. 구체적인 실시형태에 있어서, 숙주 세포 및 형질전환된 세포는 작물 식물로부터의 세포를 포함할 수 있다.
유전자전이 식물은 이어서 본 발명의 유전자전이 식물 세포로부터 재생될 수 있다. 통상의 육종 수법 혹은 자기수분을 이용해서, 종자는 이 유전자전이 식물로부터 생산될 수 있다. 이러한 종자, 및 이러한 종자로부터 성장된 얻어지는 자손 식물은, 본 발명의 재조합 DNA 분자를 포함할 것이고, 따라서 유전자전이일 것이다.
본 발명의 유전자전이 식물은 본 발명의 동형접합성 유전자전이 식물(재조합 DNA 분자를 위한 동형접합성)용의 종자를 제공하기 위하여 자가수분될 수 있거나, 또는 본 발명의 이형접합성 유전자전이 식물(재조합 DNA 분자를 위한 이형접합성)용의 종자를 제공하기 위해 교배될 수 있다. 이러한 동형접합성 및 이형접합성 유전자전이 식물은 둘 다 본 명세서에서 "자손 식물"이라 지칭된다. 자손 식물은 원래의 유전자전이 식물로부터 유래되고 본 발명의 재조합 DNA 분자를 함유하는 유전자전이 식물이다. 본 발명의 유전자전이 식물을 이용해서 생산된 종자는 유전자전이 식물의 세대, 즉, 본 발명의 작제물을 포함하고 관심대상 작물학적 유전자를 발현하는, 본 발명의 자손 식물을 성장시키기 위하여 수확되고 이용될 수 있다. 상이한 작물에 대해서 공통적으로 이용되는 육종 방법의 설명은, 여러 참고 서적, 예컨대, [Allard, Principles of Plant Breeding, John Wiley & Sons, NY, U. of CA, Davis, CA, 50-98 (1960); Simmonds, Principles of Crop Improvement, Longman, Inc., NY, 369-399 (1979); Sneep and Hendriksen, Plant breeding Perspectives, Wageningen (ed), Center for Agricultural Publishing and Documentation (1979); Fehr, Soybeans: Improvement, Production and Uses, 2nd Edition, Monograph, 16:249 (1987); Fehr, Principles of Variety Development, Theory and Technique, (Vol. 1) and Crop Species Soybean (Vol. 2), Iowa State Univ., Macmillan Pub. Co., NY, 360-376 (1987)] 중 하나에서 찾을 수 있다.
형질전환된 식물은, 관심대상 유전자 또는 유전자들의 존재 및 본 발명의 조절 요소에 의해 부여된 발현 수준 및/또는 프로파일에 대해 분석될 수 있다. 당업자는 형질전환된 식물의 분석을 위해 이용가능한 많은 방법을 알고 있다. 예를 들어, 식물 분석 방법은, 서던 블롯(Southern blot) 또는 노던 블롯(northern blot), PCR-기반 접근법, 생화학적 분석, 표현형 선별 방법, 재배지 평가 및 면역진단 검정을 포함하지만 이들로 제한되는 것은 아니다. 전사가능 DNA 분자의 발현은 제조사가 설명하는 바와 같은 택맨(TaqMan)(등록상표)(어플라이드 바이오시스템즈사(Applied Biosystems), 미국 캘리포니아주 포스터 시티 소재) 시약 및 방법 및 택맨(등록상표) 테스팅 매트릭스(Testing Matrix)를 이용하여 결정된 PCR 사이클 시간을 사용하여 측정할 수 있다. 대안적으로, 제조사가 설명하는 바와 같은 인베이더(Invader)(등록상표)(써드 웨이브 테크놀로지스사(Third Wave Technologies), 미국 위스콘신주 메디슨시 소재) 시약 및 방법이 전이유전자 발현을 위해 사용될 수 있다.
본 발명은 또한 본 발명의 식물 부위를 제공된다. 식물 부위는 잎, 줄기, 뿌리, 괴경, 종자, 배젖, 배주 및 꽃가루를 포함하지만 이들로 제한되는 것은 아니다. 본 발명의 식물 부위는 살아있고/있거나, 살아있지 않고/않거나, 재생가능하고/하거나 재생가능하지 않을 수 있다. 또한 본 발명은 본 발명의 DNA 분자를 포함하는 형질전환된 식물 세포를 포함하고 이를 제공한다. 본 발명의 형질전환된 또는 유전자전이 식물 세포는 재생가능하고/하거나 재생가능하지 않은 식물 세포를 포함한다.
본 발명은, 특정되지 않는 한, 예시로써만 제공되고 본 발명을 제한하도록 의도되지 않은 이하의 실시예들을 참조하면 더욱 용이하게 이해될 수 있다. 당업자라면, 이하의 실시예에 개시된 수법들이 본 발명의 실시에 잘 기능하도록 본 발명자들에 의해 개시된 기술을 표현하는 것을 이해해야 한다. 그러나, 당업자라면, 본 개시내용에 비추어, 개시된 구체적인 실시형태에 많은 변화가 행해질 수 있고, 본 발명의 취지와 범주로부터 벗어나는 일 없이 동일하거나 유사한 결과를 여전히 얻을 수 있고, 따라서 첨부된 도면에 제시되거나 도시된 모든 사항은 예시적인 것일 뿐 제한하는 의미로 해석되어서는 안 된다는 것을 이해할 것이다.
실시예
실시예 1
조절 요소의 확인 및 클로닝
신규한 유비퀴틴 전사 조절 요소, 또는 전사 조절 발현 요소 군(EXP) 서열을 외떡잎 식물인 구름초(Cloud grass)(아그로스티스 네불로사), 물대(giant reed)(아룬도 도낙스), 서양개밀(Blue grama)(보우텔로우아 그라실리스), 참억새(Chinese silvergrass)(미스칸투스 시네시스), 리틀 블루스템(Little bluestem)(쉬자키움 스코파륨), 옐로 인디안그래스(Yellow Indiangrass)(소가스트륨 누탄스) 및 염주(코익스 라크리마-조비(Coix lacryma-jobi))의 게놈 DNA로부터 확인하고 단리하였다.
유비퀴틴 1 전사체 서열은 상기 종들의 각각으로부터 확인되었다. 각각의 유비퀴틴 1 전사체의 5' 비번역 영역(5' UTR)은 작동 가능하게 연결된 프로모터, 리더(5' UTR) 및 제1 인트론을 포함하는 확인된 유비퀴틴 유전자에 대한 상응하는 조절 요소를 증폭시키기 위한 프라이머를 설계하기 위하여 사용되었다. 프라이머는 상응하는 게놈 DNA 서열의 5' 영역을 클로닝하기 위해 제조사의 프로토콜에 따라 작제된 게놈워커(GenomeWalker)(상표명)(클론테크 래보래토리즈사(Clontech Laboratories, Inc), 캘리포니아주 마운틴 뷰시에 소재) 라이브러리와 함께 사용되었다. 유비퀴틴 전사 조절 요소는 또한 위에 기재된 바와 같은 게놈워커(상표명) 라이브러리를 사용하여 외떡잎 식물인 세타리아 이탈리카(Setaria italica), 세타리아 비리디스(Setaria viridis) 및 제아 마이스(Zea mays) 아종 멕시카나(테오신트(Teosinte))로부터 단리되었다. 또한, 유비퀴틴 조절 요소는 유비퀴틴 4, 6 및 7 유전자에 대해서 상동체인 공개된 서열을 사용하여 외떡잎 식물인 소르굼 비콜로르(Sorghum bicolor)로부터 단리되었다.
확인된 서열을 이용하여, 증폭된 DNA 내에서 조절 요소를 확인하기 위해 생물정보학 분석을 수행하였다. 상기 분석 결과를 사용하여, 조절 요소가 DNA 서열 내에서 규정되었고, 조절 요소를 증폭하기 위한 프라이머를 설계하였다. 각각의 조절 요소에 대한 상응하는 DNA 분자는 특유한 제한 효소 부위를 함유하는 프라이머 및 아그로스티스 네불로사, 아룬도 도낙스, 보우텔로우아 그라실리스, 미스칸투스 시네시스, 쉬자키움 스코파륨, 소가스트륨 누탄스 및 코익스 라크리마-조비로부터 단리된 게놈 DNA를 사용하여 표준 중합효소 연쇄 반응(PCR) 조건을 사용하여 증폭하였다. 얻어진 DNA 단편을 기본 식물 발현 벡터 내에 결찰하고, 서열을 결정하였다. 조절 요소 전사 개시 부위(TSS) 및 인트론/엑손 스플라이스 접합부의 분석은 이어서 형질전환된 식물 원형질체를 사용하여 수행하였다. 간단하게 설명하면, 원형질체를 이종성 전사가능 DNA 분자에 작동 가능하게 연결된 클로닝된 DNA 단편을 포함하는 식물 발현 벡터로 형질전환시키고, 그에 의해 생산된 메신저 RNA(mRNA) 전사체의 서열을 분석함으로써 조절 요소 TSS 및 인트론/엑손 스플라이스 접합부를 확인하기 위해 cDNA 단부의 신속한 증폭을 위한 5' 레이스 시스템(5' RACE System for Rapid Amplification of cDNA Ends), 버전 2.0(인비트로겐사(Invitrogen), 캘리포니아주 92008 칼스배드시에 소재)을 사용하였다.
확인된 EXP의 DNA 서열은, 하기 표 1에 나열된 바와 같은, 서열번호 1, 5, 7, 9, 13, 16, 18, 19, 21, 23, 27, 30, 32, 34, 38, 41, 43, 45, 49, 52, 55, 58, 60, 62, 66, 70, 72, 74, 76, 78, 82, 84, 86, 88, 92, 95, 97, 99, 103, 106, 108, 110, 114, 116, 118, 120, 122, 126, 128, 132, 134, 138, 140, 144, 148, 150 및 168로서 본 명세서에서 제공된다. 프로모터 서열은 서열번호 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98, 100, 104, 107, 109, 111, 117, 119, 121, 123, 129, 135, 141, 145, 151 및 169로서 본 명세서에서 제공된다. 리더 서열은 서열번호 3, 11, 25, 36, 47, 64, 68, 80, 90, 101, 112, 124, 130, 136, 142, 146, 152 및 170으로서 본 명세서에서 제공된다. 인트론 서열은 서열번호 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94, 102, 105, 113, 115, 125, 127, 131, 133, 137, 139, 143, 147, 149, 153 및 171로서 본 명세서에서 제공된다.
표 1에 나타낸 바와 같이, 예를 들어, 아그로스티스 네불로사로부터 단리된 성분과 함께 EXP-AGRne.Ubq1:1:7(서열번호 1)로 지정된 조절 EXP 서열은, 인트론 요소 I-AGRne.Ubq1-1:1:3(서열번호 4)에 5' 작동 가능하게 연결된 리더 요소 L-AGRne.Ubq1-1:1:1(서열번호 3)에 5' 작동 가능하게 연결된 프로모터 요소 P-AGRne.Ubq1-1:1:5(서열번호 2)를 포함한다. 기타 EXP 서열은 표 1에 개략적으로 표시된 바와 같이, 마찬가지로 연결된다.
표 1, 서열 목록 및 도 1 내지 8에 나타낸 바와 같이, 예를 들어, P-AGRne.Ubq1-1:1:5(서열번호 2), P-ARUdo.Ubq1-1:1:4(서열번호 10)의 보다 짧은 프로모터 단편, 또는 기타 종들로부터, 예를 들어 P-AGRne.Ubq1-1:1:4(서열번호 6) 및 P-ARUdo.Ubq1-1:1:5(서열번호 14)로 되는 기타 각각의 프로모터뿐만 아니라 기타 프로모터 단편을 포함하는, 아그로스티스 네불로사, 아룬도 도낙스, 보우텔로우아 그라실리스, 미스칸투스 시네시스, 쉬자키움 스코파륨 및 소가스트륨 누탄스로부터의 프로모터 서열의 변이체가 공학적으로 조작되었다.
또한 표 1에는 제아 마이스 아종 멕시카나로부터의 게놈 DNA의 증폭을 위하여 설계된 동일한 프라이머를 이용해서 단리된 3가지 대립유전자 변이체가 열거되어 있다. 제아 마이스 아종 멕시카나 EXP 서열의 대립유전자 변이체는 다른 DNA 서열들의 각종 영역 내에 일부 동일성을 공유하는 DNA 서열로 구성되어 있지만, 삽입, 결실 및 뉴클레오타이드 부정합은 또한 각 EXP 서열의 각각의 프로모터, 리더 및/또는 인트론 내에 명백할 수 있다. EXP-Zm.UbqM1:1:6(서열번호 122) 및 EXP-Zm.UbqM1:1:10(서열번호 126)으로 지정된 EXP 서열은 제아 마이스 아종 멕시카나 Ubq1 유전자 조절 발현 요소군의 제1 대립유전자(대립유전자-1)를 나타내되, 두 EXP 서열 간의 유일한 차이는 3' 인트론 스플라이스 접합부의 서열 5'-AG-3' 이후의 각각의 인트론의 마지막 3' 뉴클레오타이드에서 발견된다. EXP-Zm.UbqM1:1:7(서열번호 128) 및 EXP-Zm.UbqM1:1:12(서열번호 132)로 지정된 EXP 서열은 제아 마이스 아종 멕시카나 Ubq1 유전자 조절 발현 요소군의 제2 대립유전자(대립유전자-2)를 나타내되, 두 EXP 서열 간의 유일한 차이는 3' 인트론 스플라이스 접합부의 서열 5'-AG-3' 이후의 각각의 인트론의 마지막 3' 뉴클레오타이드에서 발견된다. EXP 서열인 EXP-Zm.UbqM1:1:8(서열번호 134) 및 EXP-Zm.UbqM1:1:11(서열번호 138)은 제아 마이스 아종 멕시카나 Ubq1 유전자 조절 발현 요소군의 제3 대립유전자(대립유전자-3)를 나타내되, 두 EXP 서열 간의 유일한 차이는 3' 인트론 스플라이스 접합부의 서열 5'-AG-3' 이후의 각각의 인트론의 마지막 3' 뉴클레오타이드에서 발견된다.
실시예 2
GUS 발현 카세트 앰플리콘을 이용해서 옥수수 원형질체에서 GUS를 유도하는 조절 요소의 분석
옥수수 잎 원형질체를 β-글루쿠로니다제 전이유전자(GUS)의 발현을 유도하는 EXP 서열을 함유하는 식물 발현 벡터로부터 유래되는 DNA 앰플리콘으로 형질전환시키고, 이하에 제시된 일련의 실험에서 GUS의 발현이 공지의 구성적 프로모터에 의해 유도되는 잎 원형질체와 비교하였다.
제1 실험 세트에서, 잎 조직으로부터 유래된 옥수수 원형질체 세포를, EXP-AGRne.Ubq1:1:7(서열번호 1), EXP-AGRne.Ubq1:1:8(서열번호 5), EXP-AGRne.Ubq1:1:9(서열번호 7), EXP-ARUdo.Ubq1:1:8(서열번호 13), EXP-ARUdo.Ubq1:1:9(서열번호 16), EXP-ARUdo.Ubq1:1:11(서열번호 20), EXP-ARUdo.Ubq2:1:8(서열번호 26), EXP-ARUdo.Ubq2:1:9(서열번호 29), EXP-ARUdo.Ubq2:1:10(서열번호 31), EXP-BOUgr.Ubq1:1:6(서열번호 37), EXP-BOUgr.Ubq1:1:7(서열번호 40), EXP-BOUgr.Ubq1:1:8(서열번호 42), EXP-BOUgr.Ubq2:1:14(서열번호 51), EXP-BOUgr.Ubq2:1:16(서열번호 57), EXP-BOUgr.Ubq2:1:17(서열번호 59), EXP-MISsi.Ubq1:1:8(서열번호 69), EXP-MISsi.Ubq1:1:10(서열번호 71), EXP-MISsi.Ubq1:1:11(서열번호 73), EXP-MISsi.Ubq1:1:7(서열번호 75), EXP-SCHsc.Ubq1:1:9(서열번호 77), EXP-SCHsc.Ubq1:1:7(서열번호 83), EXP-SCHsc.Ubq1:1:10(서열번호 85), EXP-SORnu.Ubq1:1:6(서열번호 91), EXP-SORnu.Ubq1:1:7(서열번호 94), EXP-SORnu.Ubq1:1:8(서열번호 96), EXP-SETit.Ubq1:1:5(서열번호 102), EXP-SETit.Ubq1:1:7(서열번호 105), EXP-SETit.Ubq1:1:6(서열번호 107), EXP-Sv.Ubq1:1:7(서열번호 109), EXP-Sv.Ubq1:1:8(서열번호 115), EXP-Sv.Ubq1:1:10(서열번호 117), EXP-Zm.UbqM1:1:6(서열번호 121), EXP-Zm.UbqM1:1:7(서열번호 127), EXP-Zm.UbqM1:1:8(서열번호 133), Exp-Sb.Ubq4:1:2(서열번호 139) 및 Exp-Sb.Ubq6:1:2(서열번호 143) 중 하나에 의해 유도된 전이유전자(GUS)의 발현을 공지의 구성적 프로모터의 것과 비교하기 위하여, 식물 발현 벡터를 포함하는 GUS 발현 카세트의 증폭으로부터 생성된 앰플리콘으로 상기와 같이 형질전환시켰다. 발현 카세트 앰플리콘이 생성되는 증폭 주형을 포함하는 각 EXP 서열을, 당업계에 공지된 방법을 사용하여, "앰플리콘 주형"이라는 표제 하에 하기 표 2에 나타낸 식물 발현 벡터로 클로닝하였다. 얻어진 식물 발현 벡터는, 3' UTR T-AGRtu.nos-1:1:13(서열번호 157) 또는 T-Ta.Hsp17-1:1:1(서열번호 158)에 5' 작동 가능하게 연결된, 프로세싱 가능한 인트론("GUS-2", 서열번호 154), 또는 연속적인 GUS 암호화 서열("GUS-1", 서열번호 153)을 함유하는 GUS에 대한 암호화 서열에 5' 작동 가능하게 연결된 EXP 서열을 포함하는 발현 카세트를 포함한다. 앰플리콘은 하기 표 2에 제시된 플라스미드 작제물 주형을 사용하여 당업자에게 공지된 방법을 이용하여 생산하였다. 간단하게 설명하면, 5' 올리고뉴클레오타이드 프라이머는 프로모터 서열에 어닐링하도록 설계되었고, 3' UTR의 3' 단부에서 어닐링하는 3' 올리고뉴클레오타이드 프라이머는 각 발현 카세트의 증폭을 위해 사용되었다. 각각의 앰플리콘 주형을 포함하는 프로모터 서열 내의 상이한 위치에서 어닐링하도록 설계된 상이한 올리고뉴클레오타이드 프라이머의 사용에 의해, 연속적인 5' 결실을 발현 카세트를 포함하는 프로모터 서열 내로 도입하여 상이한 EXP 서열을 생성하였다.
표 2에서 앰플리콘 주형으로서 제시된 플라스미드 작제물은 표 2의 나열된 EXP 서열을 포함하는 전이유전자 발현 카세트의 증폭을 위한 주형으로서 제공되었다. 비교를 위한 GUS 전이유전자 앰플리콘을 생성하기 위해 사용된 대조군 플라스미드를 구성적 EXP 서열인 EXP-Os.Act1:1:9(서열번호 162) 및 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1(서열번호 161)로 앞서 기재된 바와 같이 제작하였다. 전이유전자 발현을 위하여 설계되지 않은 공백 벡터(empty vector)는 배경 GUS 및 루시페라제 발현을 평가하기 위한 음성 대조군으로서 사용하였다.
데이터의 동시-형질전환 및 정규화에서 사용하기 위한 2개의 플라스미드를 또한 당업계에 공지된 방법을 이용하여 제작하였다. 각각의 플라스미드는 구성적 EXP 서열에 의해 유도된 특이적 루시퍼라제 암호화 서열을 함유하였다. 식물 벡터 pMON19437은 아그로박테리움 투메파시엔스 노팔린 합성효소 유전자(T-AGRtu.nos-1:1:13, 서열번호 158)로부터의 3' UTR에 5' 작동 가능하게 연결된 반딧불이(포티누스 피랄리스(Photinus pyralis)) 루시퍼라제 암호화 서열(루시페라제:1:3, 서열번호 156)에 5' 작동 가능하게 연결된 인트론(EXP-CaMV.35S-enh+Zm.DnaK:1:1, 서열번호 163)에 5' 작동 가능하게 연결된 구성적 프로모터를 갖는 발현 카세트를 포함한다. 식물 벡터 pMON63934는 아그로박테리움 투메파시엔스 노팔린 합성효소 유전자(T-AGRtu.nos-1:1:13, 서열번호 158)로부터의 3' UTR에 5' 작동 가능하게 연결된 씨 팬지(sea pansy)(레닐라 레니포르미스(Renilla reniformis) 루시퍼라제 암호화 서열(CR-Ren.hRenilla Lucife-0:0:1, 서열번호 157)에 5' 작동 가능하게 연결된 구성적 EXP 서열(EXP-CaMV.35S-enh-Lhcb1, 서열번호 164)을 갖는 발현 카세트를 포함한다.
옥수수 잎 원형질체는 당업계에 잘 공지된 바와 같이 PEG-기반 형질전환 방법을 이용하여 형질전환시켰다. 원형질체 세포를 pMON19437 플라스미드 DNA, pMON63934 플라스미드 DNA 및 표 2에 제시된 앰플리콘으로 형질전환시키고, 완전한 어둠 속에서 하룻밤 인큐베이팅하였다. GUS 및 루시퍼라제 둘 모두의 측정은 상기한 바와 같이 형질전환된 세포의 용해된 제제의 분취액을 2개의 상이한 작은-웰 트레이 내에 넣음으로써 수행하였다. 하나의 트레이는 GUS 측정을 위해 사용하고, 제2 트레이는 이중 루시퍼라제 리포터 검정 시스템(프로메가사(Promega Corp.), 위스콘신주 메디슨시에 소재; 예를 들어, 문헌[Promega Notes Magazine, No: 57, 1996, p.02] 참조)을 사용하여 이중 루시퍼라제 검정을 수행하기 위해 사용하였다. 각각의 EXP 서열에 대한 하나 또는 둘의 형질전환을 수행하고, 각각의 EXP 서열에 대한 평균 발현값을 각각의 형질전환 실험으로부터의 몇몇 샘플로부터 결정하였다. 샘플 측정은 각각의 EXP 서열 작제물 형질전환의 4회 반복, 또는 대안적으로, 두 형질전환 실험 중 하나당 각각의 EXP 서열 앰플리콘의 3회 반복을 사용하여 이루어졌다. 평균 GUS 및 루시퍼라제 발현 수준을 표 3에 제공한다. 이 표에서, 반딧불이 루시퍼라제 값(예컨대, pMON19437의 발현으로부터)은 "FLuc"로 표시된 열에 제공되고, 레닐라 루시퍼라제값은 "RLuc"로 표시된 열에 제공된다.
각각의 EXP 서열의 상대적인 활성을 비교하기 위하여, GUS값들은 루시페라제 활성에 대한 GUS의 비로 표현되었고 EXP-Os.Act1:1:1 및 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1에 대해 관찰된 발현 수준에 관하여 정규화되었다. 하기 표 4는 옥수수 원형질체에서 EXP-Os.Act1:1:1 및 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 유도 발현에 대하여 정규화된 GUS/RLuc 발현 비를 나타낸다. 하기 표 5는 옥수수 원형질체에서 EXP-Os.Act1:1:1 및 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1 유도 발현에 대하여 정규화된 GUS/FLuc 발현 비를 나타낸다.
표 9 및 표 10에서 알 수 있는 바와 같이, 모든 EXP 서열은 옥수수 세포에서 GUS 전이유전자 발현을 유도할 수 있었다. 평균 GUS 발현은 EXP-Os.Act1:1:9에 대한 EXP 서열 모두에 대해서 더 높았다. EXP 서열인 EXP-AGRne.Ubq1:1:7(서열번호 1), EXP-AGRne.Ubq1:1:8(서열번호 5), EXP-AGRne.Ubq1:1:9(서열번호 7), EXP-ARUdo.Ubq1:1:8(서열번호 13), EXP-ARUdo.Ubq1:1:9(서열번호 18), EXP-ARUdo.Ubq1:1:11(서열번호 21), EXP-ARUdo.Ubq2:1:8(서열번호 27), EXP-ARUdo.Ubq2:1:9(서열번호 30), EXP-ARUdo.Ubq2:1:10(서열번호 32), EXP-BOUgr.Ubq1:1:6(서열번호 38), EXP-BOUgr.Ubq1:1:7(서열번호 41), EXP-BOUgr.Ubq2:1:14(서열번호 52), EXP-BOUgr.Ubq2:1:16(서열번호 58), EXP-BOUgr.Ubq2:1:17(서열번호 60), EXP-MISsi.Ubq1:1:10(서열번호 72), EXP-SCHsc.Ubq1:1:7(서열번호 84), EXP-SCHsc.Ubq1:1:10(서열번호 86), EXP-SORnu.Ubq1:1:7(서열번호 95), EXP-SORnu.Ubq1:1:8(서열번호 97), EXP-SETit.Ubq1:1:5(서열번호 103), EXP-SETit.Ubq1:1:7(서열번호 106), EXP-SETit.Ubq1:1:6(서열번호 108), EXP-Sv.Ubq1:1:7(서열번호 110), EXP-Sv.Ubq1:1:8(서열번호 116), EXP-Sv.Ubq1:1:10(서열번호 118), EXP-Zm.UbqM1:1:6(서열번호 122), EXP-Zm.UbqM1:1:8(서열번호 134), EXP-Sb.Ubq4:1:2(서열번호 140) 및 EXP-Sb.Ubq6:1:2(서열번호 144)은 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1의 것보다 높은 GUS 발현 수준을 나타내었다.
제2 실험 세트에서, EXP 서열 EXP-Zm.UbqM1:1:7(서열번호 128)을 함유하는 GUS 발현 카세트 앰플리콘을 GUS 발현에 관하여 대조군 앰플리콘 PCR0145942(EXP-Os.Act1:1:9, 서열번호 162) 및 PCR0145944(EXP-CaMV.35S-enh+Zm.DnaK:1:1, 서열번호 161)와 비교하였다. EXP 서열 EXP-Zm.UbqM1:1:7에 의해 유도된 GUS 발현은 두 대조군의 것보다 더 높았다. 하기 표 6은 각각의 앰플리콘에 대해 결정된 평균 GUS 및 루시퍼라제 값을 나타낸다. 하기 표 7은 옥수수 원형질체에서 EXP-Os.Act1:1:9 및 EXP-CaMV.35S-enh+Zm.DnaK:1:1 유도 발현에 대하여 정규화된 GUS/RLuc 및 GUS/FLuc 발현 비를 나타낸다.
앰플리콘으로부터 GUS 발현을 유도하는 조절 요소의 효능은 유사하게 사탕수수 잎 원형질체에서 연구될 수 있다. 예를 들어, 사탕수수 원형질체를 EXP 서열을 함유하는 식물 발현 벡터로부터 유래된 DNA 앰플리콘으로 형질전환시키고, GUS 전이유전자의 발현을 유도하고, GUS의 발현이 공지의 구성적 프로모터에 의해 유도되는 잎 원형질체와 비교할 수 있다.
실시예 3
GUS 발현 카세트 앰플리콘을 사용하여 밀 원형질체에서 GUS를 유도하는 조절 요소의 분석
밀 잎 원형질체를 EXP 서열을 함유하는 식물 발현 벡터로부터 유래된 DNA 앰플리콘으로 형질전환시키고, GUS 전이유전자의 발현을 유도시켜, GUS의 발현이 공지의 구성적 프로모터에 의해 유도된 잎 원형질체와 비교하였다.
잎 조직으로부터 유래된 밀 원형질체 세포를 당업계에 공지된 방법을 사용하여 식물 발현 벡터를 포함하는 GUS 발현 카세트의 증폭으로부터 생성된 앰플리콘으로 형질전환시켜, 상기 실시예 2에서 옥수수에서의 검정에서 사용된 바와 동일한 GUS 발현 카세트 앰플리콘을 사용하여, 선행 실시예(실시예 2)에 기재된 바와 같은 방법으로 표 3에 제시된 EXP 서열에 의해 유도된 전이유전자(GUS)의 발현을 공지의 구성적 프로모터의 것과 비교하였다. 또한, 밀 원형질체 형질전환에 사용된 대조군 GUS 발현 카세트 앰플리콘 및 루시퍼라제 플라스미드도 선행 실시예에서 제시되고 실시예 2에서 상기 표 3에 제공된 것과 동일하였다. 마찬가지로, 상기 설명된 바와 같이 GUS 및 루시퍼라제 배경의 결정을 위해 음성 대조군을 사용하였다. 밀 잎 원형질체를 상기 실시예 2에 기재된 바와 같이 PEG-기반 형질전환 방법을 이용하여 형질전환시켰다. 표 8은 형질전환된 밀 잎 원형질체 세포에서 관찰된 평균 GUS 및 LUC 활성을 나열하고, 표 9 및 표 10은 구성적 EXP 대조군에 관하여 밀 원형질체에서 정규화된 GUS/FLuc 및 GUS/RLuc 발현 비를 나타낸다.
상기 표 9 및 표 10에서 알 수 있는 바와 같이, 모든 EXP 서열은 밀 세포에서 GUS 전이유전자 발현을 유도할 수 있었다. 모든 EXP 서열은 밀 세포에서 EXP-Os.Act1:1:9보다 더 높은 수준에서 GUS 발현을 유도하였다. EXP 서열인 EXP-ARUdo.Ubq1:1:8(서열번호 13), EXP-ARUdo.Ubq1:1:9(서열번호 18), EXP-ARUdo.Ubq1:1:11(서열번호 21), EXP-ARUdo.Ubq2:1:10(서열번호 32), EXP-BOUgr.Ubq2:1:14(서열번호 52), EXP-BOUgr.Ubq2:1:16(서열번호 58), EXP-BOUgr.Ubq2:1:17(서열번호 60), EXP-MISsi.Ubq1:1:10(서열번호 72), EXP-SORnu.Ubq1:1:7(서열번호 95), EXP-SETit.Ubq1:1:5(서열번호 103), EXP-SETit.Ubq1:1:7(서열번호 106), EXP-SETit.Ubq1:1:6(서열번호 108), EXP-Sv.Ubq1:1:7(서열번호 110), EXP-Sv.Ubq1:1:8(서열번호 116), EXP-Sv.Ubq1:1:10(서열번호 118), EXP-Zm.UbqM1:1:6(서열번호 122) 및 EXP-Zm.UbqM1:1:8(서열번호 134)은, 밀 세포에서 EXP-CaMV.35S-enh+Ta.Lhcb1+Os.Act1:1:1에 의해 유도된 GUS 발현과 동등하거나 그보다 큰 GUS 발현 수준을 입증하였다.
제2 실험 세트에서, EXP-ARUdo.Ubq1:1:11(서열번호 21)을 포함하는 앰플리콘 GUS 발현 카세트는 대조군인 EXP-Os.Act1:1:9(서열번호 162) 및 EXP-CaMV.35S-enh+Zm.DnaK:1:1(서열번호 161)과 비교하였다. 하기 표 11은 각 앰플리콘에 대해서 결정된 평균 GUS 및 루시페라제값을 나타낸다. 하기 표 12는 밀 원형질체에서 EXP-Os.Act1:1:9 및 EXP-CaMV.35S-enh+Zm.DnaK:1:1 유도 발현에 대하여 정규화된 GUS/RLuc 발현 비를 나타낸다.
상기 표 12에서 알 수 있는 바와 같이, EXP-ARUdo.Ubq1:1:11(서열번호 21)에 의해 유도된 GUS 발현은 구성적 대조군인 EXP-Os.Act1:1:9 및 EXP-CaMV.35S-enh+Zm.DnaK:1:1보다 더 높았다.
실시예 4
옥수수 및 밀 원형질체에서 GUS를 유도하는 조절 요소의 분석
옥수수 및 밀 잎 원형질체를 β-글루쿠로니다제(GUS) 전이유전자의 발현을 유도하는 EXP 서열을 함유하는 식물 발현 벡터로 형질전환시키고, GUS의 발현이 공지의 구성적 프로모터에 의해 유도되는 잎 원형질체에서의 GUS 발현과 비교하였다.
EXP-Cl.Ubq10(서열번호 168)에 의해 유도된 전이유전자의 발현을 공지의 구성적 프로모터로부터의 발현과 비교하였다. 상기 EXP 서열을 하기 표 13에 표시된 바와 같은 식물 발현 벡터로 클로닝시켜, 아그로박테리움 투메파시엔스 노팔린 합성효소 유전자(T-AGRtu.nos-1:1:13, 서열번호 161) 또는 밀 Hsp17 유전자(T-Ta.Hsp17-1:1:1, 서열번호 162)로부터 유래된 3' UTR에 5' 작동 가능하게 연결된, 감자 광-유도성 조직-특이적 ST-LS1 유전자(젠뱅크(GenBank) 기탁 번호: X04753)로부터 유래된 프로세싱 가능한 인트론(GUS-2라 지칭됨, 서열번호 160)을 함유하는 GUS 리포터 또는 연속적인 GUS 암호화 서열(GUS-1, 서열번호 159)에 5' 작동 가능하게 연결된 EXP 서열을 지니는 벡터를 얻었다.
데이터의 동시-형질전환 및 정규화에서 사용하기 위한 2개의 플라스미드를 또한 당업계에 공지된 방법을 이용하여 제작하였다. 각각의 플라스미드는 구성적 EXP 서열에 의해 유도된 특이적 루시퍼라제 암호화 서열을 함유하였다. 식물 벡터 pMON19437은 아그로박테리움 투메파시엔스 노팔린 합성효소 유전자(T-AGRtu.nos-1:1:13, 서열번호 158)로부터의 3' UTR에 5' 작동 가능하게 연결된 반딧불이(포티누스 피랄리스) 루시퍼라제 암호화 서열(루시페라제:1:3, 서열번호 156)에 5' 작동 가능하게 연결된 인트론(EXP-CaMV.35S-enh+Zm.DnaK:1:1, 서열번호 163)에 5' 작동 가능하게 연결된 구성적 프로모터를 갖는 발현 카세트를 포함한다. 식물 벡터 pMON63934는 아그로박테리움 투메파시엔스 노팔린 합성효소 유전자(T-AGRtu.nos-1:1:13, 서열번호 158)로부터의 3' UTR에 5' 작동 가능하게 연결된 씨 팬지(레닐라 레니포르미스 루시퍼라제 암호화 서열(CR-Ren.hRenilla Lucife-0:0:1, 서열번호 157)에 5' 작동 가능하게 연결된 구성적 EXP 서열(EXP-CaMV.35S-enh-Lhcb1, 서열번호 164)을 갖는 발현 카세트를 포함한다.
옥수수 잎 원형질체는 당업계에 잘 공지된 바와 같이 PEG-기반 형질전환 방법을 이용하여 형질전환시켰다. 원형질체 세포를 pMON19437 플라스미드 DNA, pMON63934 플라스미드 DNA 및 표 13에 제시된 플라스미드로 형질전환시키고, 완전한 어둠 속에서 하룻밤 인큐베이팅하였다. GUS 및 루시퍼라제 둘 모두의 측정은 실시예 2에 기재된 것과 마찬가지 방식으로 행하였다. 각각의 EXP 서열에 대한 하나 또는 둘의 형질전환을 수행하고, 각각의 EXP 서열에 대한 평균 발현값을 각각의 형질전환 실험으로부터의 몇몇 샘플로부터 결정하였다. 샘플 측정은 각각의 EXP 서열 작제물 형질전환의 4회 반복, 또는 대안적으로, 두 형질전환 실험 중 하나당 각각의 EXP 서열 앰플리콘의 3회 반복을 사용하여 이루어졌다. 평균 GUS 및 루시퍼라제 발현 수준을 표 14에 제공한다. 이 표에서, 반딧불이 루시퍼라제 값(예컨대, pMON19437의 발현으로부터)은 "FLuc"로 표시된 열에 제공되고, 레닐라 루시퍼라제값은 "RLuc"로 표시된 열에 제공된다.
하기 표 15는 옥수수 원형질체에서의 EXP-Os.Act1:1:9 및 EXP-CaMV.35S-enh+Zm.DnaK:1:1 유도 발현에 대하여 정규화된 GUS/FLuc 및 GUS/RLuc 발현 비를 나타낸다.
상기 표 15에서 알 수 있는 바와 같이, EXP-Cl.Ubq10(서열번호 168)은 GUS의 발현을 유도할 수 있었지만, 두 구성적 대조군의 것보다 낮은 수준이었다.
상기 표 13에 열거된 플라스미드는 또한 위에 기재된 옥수수 잎 원형질체에 대한 것과 마찬가지 방식으로 밀 잎 원형질체 세포를 형질전환시켰다. 평균 GUS 및 루시페라제값은 하기 표 16에 표시되어 있다. 하기 표 17은 옥수수 원형질체에서 EXP-CaMV.35S-enh+Zm.DnaK:1:1에 대하여 정규화된 GUS/FLuc 및 GUS/RLuc 발현 비를 나타낸다.
상기 표 17에서 알 수 있는 바와 같이, EXP-Cl.Ubq10(서열번호 168)은, 밀 원형질체 세포에서 EXP-CaMV.35S-enh+Zm.DnaK:1:1(서열번호 160)의 것과 유사한 수준에서 GUS를 발현하였다.
실시예 5
유전자전이 옥수수에서 GUS를 유도하는 조절 요소의 분석.
옥수수 식물을 GUS 전이유전자의 발현을 유도하는 EXP 서열을 함유하는 식물 발현 벡터로 형질전환시키고, 얻어진 식물을 GUS 단백질 발현에 대해서 분석하였다. 유비퀴틴 EXP 서열은 당업계에 공지된 방법을 이용해서 식물 이원성 형질전환 플라스미드 작제물 내로 클로닝하였다.
얻어지는 식물 발현 벡터는, 아그로박테리움 투메파시엔스로부터의 우측 경계 영역; 벼 지질 전달 단백질 유전자(T-Os.LTP-1:1:1, 서열번호 159)로부터의 3' UTR에 5' 작동 가능하게 연결된 상기 설명된 프로세싱가능한 인트론 GUS-2를 갖는 GUS에 대한 암호화 서열에 작동 가능하게 연결된 EXP 서열을 검정하기 위한 제1 발현 카세트; 제초제 글라이포세이트(벼 액틴 1 프로모터에 의해 유도됨)에 대한 내성을 부여하는 형질전환된 식물 세포의 선택을 위해 사용된 제2 전이유전자 선택 카세트; 및 아그로박테리움 투메파시엔스로부터의 좌측 경계 영역을 함유한다. 생성되는 플라스미드는 옥수수 식물을 형질전환시키기 위해 사용되었다. 표 18은 플라스미드 지정, EXP 서열 및 서열 번호를 나열하고, 이는 또한 표 1에 기재되어 있다.
식물을, 예를 들어, 미국 특허 출원 공개 제2009/0138985호에 설명된 바와 같이, 아그로박테리움-매개 형질전환을 이용하여 형질전환시켰다.
형질전환된 식물의 정성적 발현 분석을 위해 조직화학적 GUS 분석을 이용하였다. 전체 조직 절편을 GUS 염색 용액 X-Gluc(5-브로모-4-클로로-3-인돌릴-b-글루쿠로나이드)(1 ㎎/㎖)와 함께 적절한 정도의 시간 동안 인큐베이팅하고, 세정하고, 청색 착색에 대해 시각적으로 검사하였다. GUS 활성은 선택된 식물 기관 및 조직을 이용하여 직접 시각 검사에 의해 또는 현미경 하 검사에 의해 정성적으로 결정하였다. R0 식물을 뿌리 및 잎뿐만 아니라 꽃밥, 견사(silk) 및 발생하는 종자와 수분(pollination) 21일 후(21 DAP) 배에서 발현에 대해 검사하였다.
정량적 분석을 위해, 형질전환된 옥수수 식물의 선택된 조직으로부터 총 단백질을 추출하였다. 1 마이크로그램의 총 단백질을 형광 기질인 4-메틸움벨리페릴-β-D-글루쿠로나이드(MUG)와 함께 50㎕의 총 반응 부피로 사용하였다. 반응 생성물인 4-메틸움벨리페론(4-MU)은 하이드록실기가 이온화되는 높은 pH에서 최대로 형광성이다. 탄산나트륨의 염기성 용액을 동시에 첨가하여 검정을 중지시키고 형광 생성물을 정량하기 위해 pH를 조정하였다. 형광을 여기 2㎚ 및 방출 3㎚로 설정된 슬릿(slit) 폭을 갖는, 마이크로맥스 판독기(Micromax Reader)로 플루오로맥스(Fluoromax)-3(호리바사(Horiba), 일본 쿄토에 소재)을 사용하여 365㎚에서의 여기 및 445㎚에서의 방출로 측정하였다.
각각의 형질전환에 대해 관찰된 평균 R0 GUS 발현은 하기 표 19 및 20에 제시되어 있다.
R0 옥수수 식물에서, 잎과 뿌리에서의 GUS 발현 수준은 유비퀴틴 EXP 서열들 간에 상이하였다. 모든 EXP 서열은, 안정적으로 형질전환된 식물에서 GUS 전이유전자 발현을 유도하는 능력을 나타낸 한편, 각각의 EXP 서열은 다른 것들에 비해 독특한 발현 패턴을 나타내었다. 예컨대, EXP 서열인 EXP-AGRne.Ubq1:1:7(서열번호 1), EXP-AGRne.Ubq1:1:8(서열번호 5), EXP-ARUdo.Ubq1:1:8(서열번호 13), EXP-ARUdo.Ubq1:1:9(서열번호 18), EXP-ARUdo.Ubq2:1:8(서열번호 27), EXP-ARUdo.Ubq2:1:9(서열번호 30), EXP-BOUgr.Ubq1:1:6(서열번호 38), EXP-BOUgr.Ubq1:1:7(서열번호 41), EXP-BOUgr.Ubq2:1:14(서열번호 52), EXP-BOUgr.Ubq2:1:15(서열번호 55), EXP-MISsi.Ubq1:1:7(서열번호 76), EXP-SORnu.Ubq1:1:6(서열번호 92), EXP-SORnu.Ubq1:1:7(서열번호 95), EXP-SETit.Ubq1:1:10(서열번호 99), EXP-Sv.Ubq1:1:11(서열번호 114), EXP-Zm.UbqM1:1:12(서열번호 132) 및 EXP-Sb.Ubq7:1:2(서열번호 150)는 EXP-Sv.Ubq1:1:12(서열번호 120), EXP-Zm.UbqM1:1:10(서열번호 126), EXP-Zm.UbqM1:1:11(서열번호 138), EXP-Sb.Ubq4:1:2(서열번호 140) 및 EXP-Sb.Ubq6:1:3(서열번호 148)에 비해서 발생 단계인 V3 및 V7 단계에서 보다 낮은 수준의 뿌리의 GUS 발현을 나타내었다. 보다 높은 수준의 GUS 발현이 EXP-ARUdo.Ubq1:1:8(서열번호 13), EXP-ARUdo.Ubq1:1:9(서열번호 18), EXP-BOUgr.Ubq1:1:7(서열번호 41), EXP-Zm.UbqM1:1:11(서열번호 138) 및 EXP-Sb.Ubq6:1:3(서열번호 148)에 대한 뿌리 발생 후기 단계(VT)에서 관찰되었다. EXP-Zm.UbqM1:1:10(서열번호 140)에 의해 유도된 뿌리 발현은 V3에서 발현을 보이지 않았지만 V7에서 높았으며, 그 후 VT 단계에서 하강하였다. EXP-Zm.UbqM1:1:11(서열번호 150)에 의해 유도된 뿌리 발현은 V3 단계로부터 V7 단계 내지 VT 단계까지 발달 전체를 통해 유사한 수준으로 유지되었다. EXP-Cl.Ubq10(서열번호 168)에 의해 유도된 GUS의 발현은 V4 단계에서 V7 단계까지 비교적 규칙적이었지만, VT 단계에서는 V4 및 V7 단계의 것의 대략 절반으로 떨어졌다.
GUS 발현 수준은 잎 조직에서 또한 극적인 차이를 보였다. EXP 서열인 EXP-Zm.UbqM1:1:10(서열번호 126), EXP-Zm.UbqM1:1:12(서열번호 132) 및 EXP-Zm.UbqM1:1:11(서열번호 138)은 세 발단 단계(V3, V7 및 VT) 모두에 걸쳐서 최고 수준의 GUS 발현이 관찰된 것을 나타내었다. EXP 서열인 EXP-Sb.Ubq4:1:2(서열번호 140)는, V3에서 VT까지의 발단 단계에서 발현의 감소를 보였다. EXP 서열인 EXP-ARUdo.Ubq1:1:8(서열번호 13) 및 EXP-Sb.Ubq7:1:2(서열번호 150)는 V3 및 VT 단계에서 보다 높은 수준의 GUS 발현을 보였고, V7 단계에서 성장의 중간에서 보다 낮은 수준의 발현을 보였다. EXP 서열인 EXP-ARUdo.Ubq2:1:9(서열번호 30), EXP-BOUgr.Ubq1:1:7(서열번호 41) 및 EXP-MISsi.Ubq1:1:7(서열번호 76)은 세 단계 모두에 걸쳐서 GUS 발현을 유지한 반면, EXP-ARUdo.Ubq2:1:8(서열번호 27), EXP-BOUgr.Ubq1:1:6(서열번호 38) 및 EXP-BOUgr.Ubq2:1:15(서열번호 55)는 VT 단계에서 발현의 약간 감소를 보였다. EXP-Cl.Ubq10(서열번호 168)에 의해 유도된 발현은 V4 및 VT 단계에서 유사하였지만, V7 단계에서 V4 및 VT의 대략 절반 수준으로 떨어졌다.
마찬가지로, 생식 조직(꽃밥 및 견사)에 관하여, 상이한 발현이 패턴이 각각의 EXP 서열에 독특하게 관찰되었다. 예를 들어, EXP 서열인 EXP-ARUdo.Ubq1:1:8(서열번호 13), EXP-ARUdo.Ubq1:1:9(서열번호 18), EXP-BOUgr.Ubq2:1:14(서열번호 52), EXP-BOUgr.Ubq2:1:15(서열번호 55), EXP-SORnu.Ubq1:1:7(서열번호 95), EXP-Zm.UbqM1:1:10(서열번호 126), EXP-Zm.UbqM1:1:12(서열번호 132), EXP-Zm.UbqM1:1:11(서열번호 138) 및 EXP-Sb.Ubq7:1:2(서열번호 150)에 대해서 꽃밥 및 견사에서 높은 수준의 발현이 관찰되었다. EXP 서열인 EXP-AGRne.Ubq1:1:7(서열번호 1), EXP-SORnu.Ubq1:1:6(서열번호 92), EXP-Sv.Ubq1:1:11(서열번호 114), EXP-Sv.Ubq1:1:12(서열번호 120), EXP-Sb.Ubq4:1:2(서열번호 140), EXP-Sb.Ubq6:1:3(서열번호 148) 및 EXP-Cl.Ubq10(서열번호 168)에 의해 유도된 발현은 꽃밥에서 높았지만 각 EXP 서열에 비해서 견사에서 낮은 한편, EXP-BOUgr.Ubq1:1:6(서열번호 38)에 의해 유도된 발현은 꽃밥에서의 발현에 비해서 견사에서 더 높았다.
발생하는 종자(21 DAP 배 및 배젖)에서의 발현은 EXP 서열들 간에 상이하였다. EXP 서열인 EXP-Zm.UbqM1:1:10(서열번호 126), EXP-Zm.UbqM1:1:12(서열번호 132) 및 EXP-Zm.UbqM1:1:11(서열번호 138)은 발생하는 종자 배 및 배젖 조직에서 GUS의 높은 발현을 유도하였다. 배젖에서의 발현 수준은, GUS가 EXP 서열인 EXP-ARUdo.Ubq1:1:8(서열번호 13), EXP-ARUdo.Ubq1:1:9(서열번호 18), EXP-ARUdo.Ubq2:1:8(서열번호 27), EXP-BOUgr.Ubq2:1:14(서열번호 52), EXP-BOUgr.Ubq2:1:15(서열번호 55), EXP-SORnu.Ubq1:1:6(서열번호 92), EXP-SORnu.Ubq1:1:7(서열번호 95), EXP-Sv.Ubq1:1:12(서열번호 120), EXP-Sb.Ubq4:1:2(서열번호 140) 및 EXP-Cl.Ubq10(서열번호 168)에 의해 유도된 경우의 배에서보다 약 2배 이상 높았다. EXP-Sb.Ubq7:1:2(서열번호 150)에 의해 유도된 경우 GUS의 발현은 배젖에서보다 배에서 3배 더 높았다. GUS 발현 수준은 EXP 서열인 EXP-AGRne.Ubq1:1:7(서열번호 1), EXP-AGRne.Ubq1:1:8(서열번호 5), EXP-BOUgr.Ubq1:1:6(서열번호 38), EXP-BOUgr.Ubq1:1:7(서열번호 41), EXP-MISsi.Ubq1:1:7(서열번호 76), EXP-SETit.Ubq1:1:10(서열번호 99) 및 EXP-Sb.Ubq6:1:3(서열번호 148)에 의해 유도된 경우 배 및 배젖에서 비교적 동등하였다.
각각의 EXP 서열은 안정적으로 형질전환된 옥수수 식물에서 전이유전자 발현을 유도하는 능력을 나타내었다. 그러나, 각각의 EXP 서열은, 독특한 각 조직에 대한 발현 패턴을 지녔으며, 목적으로 하는 결과를 달성하기 위하여 필요로 되는 조직 발현 전략에 따라서 특정 전이유전자의 발현을 최상으로 제공할 EXP 서열을 선택할 기회를 제공한다. 이 실시예는 상동성 유전자로부터 단리된 EXP 서열이 형질전환된 식물에서 반드시 동등하게 거동하지는 않고, 발현은 각각의 EXP 서열에 대한 특성의 실험에 의한 조사를 통해서만 결정될 수 있고 그로부터 프로모터가 유래되는 유전자 상동성에 기반하여 예측될 수 없음을 입증한다.
실시예 6
조절 요소로부터 유래된 인핸서.
인핸서는 본 명세서에서 제공된 프로모터 요소, 예컨대, 서열번호 2, 6, 8, 10, 14, 17, 22, 24, 28, 31, 33, 35, 39, 42, 44, 46, 50, 53, 56, 61, 63, 67, 71, 73, 75, 77, 79, 83, 85, 87, 89, 93, 96, 98 및 169로서 제시된 것들로부터 유래된다. 인핸서 요소는, 프로모터 요소에 5' 또는 3' 작동 가능하게 연결될 때, 또는 프로모터에 작동 가능하게 연결된 추가의 인핸서 요소에 5' 또는 3' 작동 가능하게 연결될 때, 특정 세포 종류 또는 식물기관 내에서 또는 발달 또는 일주기성 리듬의 특정 시점에서 전이유전자의 발현을 향상 또는 조정하거나 전이유전자의 발현을 제공할 수 있는 하나 이상의 시스 조절 요소로 구성될 수 있다. 인핸서는 그의 단편을 비롯하여 위에서 설명된 바와 같이 본 명세서에서 제공된 프로모터로부터 전사가 개시되도록 허용하는 프로모터로부터, TATA 박스 또는 기능상 유사한 요소 및 임의의 하류 DNA 서열을 제거함으로써 제조하였고, 여기서 TATA 박스 또는 기능상 유사한 요소 및 TATA 박스의 하류의 DNA 서열이 제거된다.
인핸서 요소는 본 명세서에서 제공된 프로모터 요소로부터 유래되고, 당업계에 공지된 방법을 이용하여 프로모터 요소에 5' 또는 3' 작동 가능하게 연결되거나 프로모터에 작동 가능하게 연결된 추가의 인핸서 요소에 5' 또는 3' 작동 가능하게 연결되도록 클로닝될 수 있다. 대안적으로, 인핸서 요소를 당업계에 공지된 방법을 이용하여 프로모터 요소에 5' 또는 3' 작동 가능하게 연결된, 또는 프로모터에 작동 가능하게 연결된 추가의 인핸서 요소에 5' 또는 3' 작동 가능하게 연결된 인핸서 요소의 하나 이상의 카피에 작동 가능하게 연결되도록 클로닝하였다. 인핸서 요소는 또한 상이한 속의 유기체로부터 유래된 프로모터 요소에 5' 또는 3' 작동 가능하게 연결되거나, 또는 동일한 또는 상이한 속의 유기체로부터 유래된 프로모터에 작동 가능하게 연결된 다른 속의 유기체 또는 동일한 속의 유기체로부터 유래된 추가의 인핸서 요소에 5' 또는 3' 작동 가능하게 연결되어, 결과적으로 키메라 조절 요소를 생성하도록 클로닝될 수 있다. GUS 발현 식물 형질전환 벡터를 선행 실시예에 설명된 작제물과 유사한 당업계에 공지된 방법을 사용하여 제작하였고, 여기서 생성되는 식물 발현 벡터는, 아그로박테리움 투메파시엔스로부터의 우측 경계 영역; 아그로박테리움 투메파시엔스로부터의 노팔린 합성효소 3' UTR(T-AGRtu.nos-1:1:13, 서열번호 158) 또는 벼 지질 전달 단백질 유전자(T-Os.LTP-1:1:1, 서열번호 160)로부터의 3' UTR에 작동 가능하게 연결된, 프로세싱가능한 인트론(GUS-2, 서열번호 155)을 갖거나 인트론을 갖지 않는(GUS-1, 서열번호 154) GUS에 대한 암호화 서열에 작동 가능하게 연결된, 제아 메이즈의 HSP70 열 충격 단백질(I-Zm.DnaK-1:1:1 서열번호 165)로부터 유래된 인트론 또는 본 명세서에 제시된 임의의 인트론 또는 임의의 다른 인트론에 작동 가능하게 연결된, 조절 또는 키메라 조절 요소를 포함하는 조절 또는 키메라 조절 요소를 시험하기 위한 제1 발현 카세트; 제초제 글라이포세이트(벼 액틴 1 프로모터에 의해 유도됨), 또는 대안적으로 항생제 카나마이신(벼 액틴 1 프로모터에 의해 유도됨)에 대한 내성을 부여하는 형질전환된 식물 세포의 선택을 위해 사용되는 제2 전이유전자 선택 카세트, 및 아그로박테리움 투메파시엔스로부터 좌측 경계 영역을 함유한다. 얻어지는 플라스미드는 위에서 설명된 방법에 의해 또는 당업계에 공지된 다른 아그로박테리움-매개 또는 입자 충격 (bombardment) 방법에 의해 옥수수 식물 또는 다른 속의 식물을 형질전환시키기 위해 사용된다. 대안적으로, 옥수수 또는 다른 속의 식물로부터 유래된 원형질체 세포는 일시적 검정을 수행하기 위해 당업계에 공지된 방법을 이용하여 형질전환된다.
하나 이상의 인핸서를 포함하는 조절 요소에 의해 유도된 GUS 발현을, 전이유전자의 발현에 대한 인핸서 요소의 효과를 결정하기 위해 안정한 또는 일시적 식물 검정에서 평가하였다. 하나 이상의 인핸서 요소에 대한 변형 또는 하나 이상의 인핸서 요소의 복제를 경험적 실험에 기반하여 수행하고, 얻어지는 유전자 발현 조절을 각각의 조절 요소 조성물을 사용하여 관찰하였다. 얻어지는 조절 또는 키메라 조절 요소 내에서 하나 이상의 인핸서의 상대적인 위치를 변경시키면 조절 또는 키메라 조절 요소의 전사 활성 또는 특이성에 영향을 미칠 수 있고, 옥수수 식물 또는 다른 속의 식물 내에서 목적으로 하는 전이유전자 발현 프로파일을 위한 최상의 인핸서를 확인하기 위해 실험으로 결정하였다.
실시예 7
식물 유래 원형질체를 사용하는 GUS 활성의 인트론 향상의 분석.
인트론은 전이유전자의 최적 발현을 위한 벡터 전달 DNA(T-DNA) 요소 배열 내에서 인트론 및 구성을 실험으로 선택하기 위해 인트론이 없는 발현 벡터 대조군과의 비교 및 실험에 기반하여 선택한다. 예를 들어, 글라이포세이트에 대한 내성을 부여하는 CP4와 같은 제초제 내성 유전자의 발현에서, 제초제를 시비할 때 수확량의 손실을 방지하기 위해 생식 조직뿐만 아니라 생장 조직 내에서 전이유전자 발현을 갖는 것이 바람직하다. 이 경우에, 인트론은, 구성적 프로모터에 작동 가능하게 연결될 때, 특히 유전자전이 식물의 생식 세포 및 조직 내에서 제초제 내성 부여 전이유전자의 발현을 향상시키는, 이에 따라서 제초제를 분무할 때 유전자전이 식물에 생장 및 생식 내성을 모두 제공하는 그의 능력에 따라 선택될 것이다. 대부분의 유비퀴틴 유전자 내에서, 5' UTR은 그 내부에 인트론 서열이 묻혀 있는 리더를 포함한다. 따라서, 그러한 유전자로부터 유래된 발현 요소는 프로모터, 리더, 및 인트론을 포함하는 전체 5' UTR을 사용하여 검정하였다. 상이한 발현 프로파일을 달성하기 위해 또는 전이유전자 발현의 수준을 조정하기 위해, 그러한 발현 요소로부터의 인트론을 제거하거나 이종성 인트론으로 치환시킬 수 있다.
서열번호 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 및 171로서 본 명세서에서 제시된 인트론은 발현된 서열 태그 클러스터에 비해서 게놈 DNA 콘티그(contig) 또는 게놈 DNA 내에서 엑손 및 인트론 서열을 확인하기 위해 cDNA 콘티그를 사용하여 확인되었다. 추가로, 5' UTR 또는 리더 서열은 또한 유전자 서열이 하나 이상의 인트론이 개재된 리더 서열을 암호화하는 조건 하에 하나 이상의 인트론의 인트론/엑손 스플라이스 접합부를 규정하기 위해 사용되었다. 인트론을 당업계에 공지된 방법을 이용하여, 예를 들어, 도 9에 제시된 발현 카세트에 도시된 바와 같이, 조절 요소 및 리더 단편에 3' 작동 가능하게 연결되고 제2 리더 단편 또는 암호화 서열에 5' 작동 가능하게 연결되도록 식물 형질전환 벡터 내로 클로닝하였다.
따라서, 예를 들어, 제1의 가능한 발현 카세트(도 9에서의 발현 카세트 구성 1)는, 3' UTR 요소[E]에 작동 가능하게 연결된 암호화 영역[D]에 작동 가능하게 연결된 시험 인트론 요소[C]에 5' 작동 가능하게 연결된 리더 요소[B]에 5' 작동 가능하게 연결된 프로모터 또는 키메라 프로모터 요소[A]로 구성된다. 대안적으로, 제2의 가능한 발현 카세트(도 9에서의 발현 카세트 구성 2)는 3' UTR 요소[K]에 작동 가능하게 연결된 암호화 영역[J]에 작동 가능하게 연결된 제2 리더 요소 또는 제1 리더 요소의 제2 단편[I]에 5' 작동 가능하게 연결된 시험 인트론 요소[H]에 5' 작동 가능하게 연결된 제1 리더 요소 또는 제1 리더 요소 단편[G]에 5' 작동 가능하게 연결된 프로모터 또는 키메라 프로모터 요소[F]로 구성된다. 또한, 제3의 가능한 발현 카세트(도 9에서의 발현 카세트 구성 3)는 3' UTR 요소[Q]에 작동 가능하게 연결된 암호화 서열 요소[P]의 제2 단편에 5' 작동 가능하게 연결된 인트론 요소[O] 요소에 5' 작동 가능하게 연결된 암호화 서열 요소[N]의 제1 단편에 5' 작동 가능하게 연결된 리더 요소[M]에 5' 작동 가능하게 연결된 프로모터 또는 키메라 프로모터 요소[L]로 구성된다. 발현 카세트 구성 3은 암호화 서열의 제1 단편과 제2 단편 간의 프레임 이동 없이 완전한 개방 해독틀(open reading frame)을 생산하는 방식으로 인트론의 스플라이싱을 허용하도록 설계된다.
위에서 논의된 바와 같이, 메신저 RNA의 최종 전사체로의 프로세싱 동안 원치 않는 개시 코돈이 형성될 가능성을 제거하기 위해, 스플라이스 부위의 5' 단부(GT) 바로 앞의 뉴클레오타이드 서열 AT 또는 뉴클레오타이드 A, 및 스플라이스 부위의 3' 단부(AG)의 바로 다음의 각각 뉴클레오타이드 G 또는 뉴클레오타이드 서열 TG의 사용을 피하는 것이 바람직할 수 있다. 따라서, 인트론의 5' 또는 3' 단부 스플라이스 접합부 부위 주위의 DNA 서열은 변형될 수 있다.
인트론을 일시적 검정 또는 안정한 식물 검정에서 발현을 향상시키는 능력을 통한 향상 효과에 대해 검정한다. 인트론 향상의 일시적 검정을 위해, 기본 식물 벡터를 당업계에 공지된 방법을 이용하여 제작하였다. 인트론을, 아그로박테리움 투메파시엔스로부터의 노팔린 합성효소 3' UTR(T-AGRtu.nos-1:1:13, 서열번호 158)에 작동 가능하게 연결된, 프로세싱가능한 인트론(GUS-2, 서열 155)을 갖거나 인트론을 갖지 않는(GUS-1, 서열 154) GUS에 대한 암호화 서열에 작동 가능하게 연결된 시험 인트론 요소(예컨대, 서열번호 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 및 171 중 하나)에 5' 작동 가능하게 연결된 리더 요소 L-CaMV.35S-1:1:15(서열번호 167)에 5' 작동 가능하게 연결된 꽃양배추 모자이크 바이러스 프로모터 P-CaMV.35S-enh-1:1:9(서열번호 166) 등과 같은 구성적 프로모터를 포함하는 발현 카세트를 포함하는 기본 식물 벡터 내로 클로닝시킨다. 옥수수 또는 다른 속의 식물 조직으로부터 유래된 원형질체 세포를 상기 실시예 2에서 앞서 설명된 바와 같이 기본 식물 벡터 및 루시퍼라제 대조군 벡터로 형질전환시키고, 활성에 대해 검정하였다. 발현을 향상시키는 인트론의 상대적인 능력을 비교하기 위해, GUS 값을 GUS 대 루시퍼라제 활성의 비로서 표현하고, 공지의 인트론 표준물에 작동 가능하게 연결된 구성적 프로모터를 포함하는 작제물, 예컨대 제아 메이즈의 HSP70 열 충격 단백질로부터 유래된 인트론 I-Zm.DnaK-1:1:1(서열번호 165)뿐만 아니라 구성적 프로모터를 포함하지만 프로모터에 작동 가능하게 연결된 인트론이 없는 작제물에 의해 부여된 수준과 비교하였다.
서열번호 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 및 171로서 제시된 인트론의 안정적인 식물 검정을 위해, 선행 실시예에 설명된 작제물과 유사한 GUS 발현 식물 형질전환 벡터를 제작하고, 여기서 얻어지는 식물 발현 벡터는, 아그로박테리움 투메파시엔스로부터의 우측 경계 영역; 아그로박테리움 투메파시엔스로부터의 노팔린 합성효소 3' UTR(T-AGRtu.nos-1:1:13, 서열번호 158)에 작동 가능하게 연결된 프로세싱 가능한 인트론(GUS-2, 서열번호 155)을 지니거나 인트론을 지니지 않는(GUS-1, 서열번호 154) GUS에 대한 암호화 서열에 작동 가능하게 연결된 본 명세서에 제시된 시험 인트론 요소에 5' 작동 가능하게 연결된 리더 요소 L-CaMV.35S-1:1:15(서열번호 167)에 5' 작동 가능하게 연결된 꽃양배추 모자이크 바이러스 프로모터 P-CaMV.35S-enh-1:1:9(서열번호 166) 등과 같은 구성적 프로모터를 포함하는 인트론을 시험하기 위한 제1 발현 카세트; 글라이포세이트(벼 액틴 1 프로모터에 의해 유도됨), 또는 대안적으로 항생제 카나마이신(벼 액틴 1 프로모터에 의해 유도됨)에 대한 내성을 부여하는 형질전환된 식물 세포의 선택을 위해 사용되는 제2 전이유전자 선택 카세트, 및 아그로박테리움 투메파시엔스로부터의 좌측 경계 영역을 함유한다. 얻어지는 플라스미드는 상기 설명된 방법에 의해 또는 당업계에 공지된 아그로박테리움-매개된 방법에 의해 옥수수 식물 또는 다른 속의 식물을 형질전환시키기 위해 사용된다. 단일 카피 또는 낮은 카피수 형질전환체는 시험 인트론이 인트론 매개된 향상 효과를 제공하는지를 결정하기 위해 시험 벡터와 동일한 식물 형질전환 벡터를 사용하지만 시험 인트론이 없이 형질전환된 단일 카피 또는 낮은 카피수 형질전환된 식물에 비교하기 위해 선택된다.
서열번호 4, 12, 15, 20, 26, 29, 37, 40, 48, 51, 54, 57, 59, 65, 69, 81, 91, 94 및 171에서 제시된 인트론의 어느 것이라도 인트론 서열 내에서 단편을 결실시키는 것과 같은 많은 방식으로 변형될 수 있고, 이것은 발현을 향상시킬 수 있는, 인트론 내의 단편의 발현 또는 복제를 감소시킬 수 있다. 추가로, 특정 세포 종류 또는 조직 및 기관에 대해 발현의 특이성에 영향을 미칠 수 있는 인트론 내의 DNA 서열은 전이유전자의 발현 및 발현의 패턴에 영향을 미치도록 복제되거나 변경되거나 결실될 수 있다. 또한, 본 명세서에서 제공되는 인트론은 임의의 잠재적인 개시 코돈(ATG)을 제거하도록 변형될 수 있고, 이것은 의도하지 않은 전사체가 부적절하게 스플라이싱된 인트론으로부터 상이한, 보다 긴 또는 말단절단된 단백질로서 발현되도록 할 수 있다. 일단 인트론이 실험에 의해 시험되거나 실험에 기반하여 변경된 후, 인트론은, 해당 인트론이 전이유전자의 향상을 제공하는 한, 임의의 속의 외떡잎식물 또는 쌍떡잎 식물일 수 있는 안정적으로 형질전환된 식물 내에서 전이유전자의 발현을 향상시키기 위해 사용된다. 인트론은 또한, 해당 인트론이 그에 작동 가능하게 연결된 전이유전자의 발현의 향상 또는 감쇠 또는 특이성을 제공하는 한, 다른 유기체, 예컨대 조류, 진균 또는 동물 세포에서 발현을 향상시키기 위해 사용될 수 있다.
* * * * * * *
본 발명의 원리를 예시하고 설명하였지만, 본 발명은 이러한 원리를 벗어나는 일 없이 배열 및 상세가 변형될 수 있음은 당업자에게 명백할 것이다. 본 발명자들은 특허청구범위의 취지와 범위 내에 있는 모든 변형을 청구한다. 본 명세서에서 인용한 모든 간행물 및 간행된 특허 문서는 마치 각각의 개별 간행물 또는 특허 출원이 참고로 편입되는 것으로 구체적이고도 개별적으로 지시되는 것과 동일한 정도로 본 명세서에 참고로 포함된다.
<110> Monsanto Technology LLC
<120> PLANT REGULATORY ELEMENTS AND USES THEREOF
<130> MONS:323WO
<140> Unknown
<141> 2013-12-17
<150> 61/739,720
<151> 2012-12-19
<160> 171
<210> 1
<211> 3143
<212> DNA
<213> Agrostis nebulosa
<400> 1
ggcctcttta cgtttggcac aatttaattg aatcccggca tggcatgtta gaccggagtg 60
agccggccct tttactggta tgacactccc tctgtcttga gtgtcgctgt gccagcttgt 120
acctctgtct atgttcacag cccgtgctgt gtacctagac cctccgtttg tccacattca 180
ttttaatctc tattgtatct tgtcaaaacc taaaagccta aaacgactct gataaaggga 240
cagaaagatt atacaagagc aagtgtataa tgaaataatg taagcgagct atatgaattg 300
tcacgtgtca tatttatgtt gagacgaaga agagaaaata aacaccatgc aaatttatgg 360
cgagtgatag atggccagat gggcacaagg cctcctattt cttaaatcgg attttgtaag 420
aacgaaaaaa gggacttata agagaatagg atagaccata tatcaatgat gtagtatgca 480
tcaagatcta actattatat gagtgaattg ataaatttat tctaggtgac atggccttaa 540
cgatgaacag tacatggtta aatcaataga acaatagcca actctagcag ctctaaaaaa 600
agatatatat tcgtcgaggc actattatgc aaccacatag tcaacttcaa caccgcttga 660
gtgcgttctc atgttttttt tttcttgcaa attacgcttt tctaaaataa aataatttgg 720
atcgtgcaat tatttcactt taggtgtgcg tgactacgtg agtaacattt ttgaatctca 780
gaaaggaaat aaaagtataa tactgctgcc tactttgagg attcggcttg ttatttaaaa 840
ccgtctttaa ggtcaaatgc tcaagattca ttcaacaatt gaaacgtctc acatgattaa 900
atcatgtata aggatgctaa ggtcttgctt gacaatgttt ttctaggaat ttcatctaac 960
tttttgagtg aaactatcaa ataataattt taaaacaatt ttataagaga agctccggag 1020
ataaaaggcc atctaatcta tgttagaaga gtgaagttta ctccctctgt cccaaaaata 1080
gaattctaag tatgaaatga tttttttgtt atacaaaagg agtatatatc acaagattga 1140
tgtcagttat gcttagggca cgtacacgac gctggtgctt taggtagacg ttaatcgttg 1200
tttctgcatt ttattttatt ttgttgccac ggtgtacatt tgggtagacg tttgtcacag 1260
gcattgccac tcaaacaagc agccggcgct tggagctttt atagtttgaa aagtgacggt 1320
tttaaggatg ggtaagctga ttagtatatg taagtttagc tttttccatt gtaggttaag 1380
ccttaaggct cttacacaat tgtttcatta ttctcattct ttaagagccc atataagcgt 1440
tcatgaattg tacatatcct tagatttttt tttttgggta aagctcgagc ttctgtatct 1500
aaaagtagag aaatcagaaa aagattcatg ttttggtagt tttgatttct tgcctccata 1560
ataattttgg tttaccattt tttgtttgat tttagtttta gaagcgttta tagcaggatt 1620
taaaatccaa aactaccatt atcttcaagt gaccgtcagt gagccgttta acggcgtcga 1680
caagtccaac ggacaccaac cagtgaacca ccagcgtcga gccaagcgat gcaaacggaa 1740
cggccgagac gttgacacct ttggcgcggc acggcatgtc ggatctccct ctctggcccc 1800
ctctcgagag ttccagctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt 1860
tccgcctcct gcctgctcct ctcagacggc acgaaaccgt gacggcaccg gcagcacggg 1920
gggattcctt ttccactgct ccttcctttt cccttcctcg cccgccgcta taaatagcca 1980
gccccgtccc cagattcttt cccaacctca tctttgttcg gagcacccac acaacccgat 2040
ccccaattcc ctcgtctctc ctcgcgagcc tcgtcgaccc ccccttcaag gtacggcgat 2100
cgtcctccct ccctctctct ctctaccttc tcttctctag actagatcgg cgacccggtc 2160
catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg taaaatagat 2220
ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc ctttaggaca 2280
tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct aggcagtggg 2340
gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa ctgggaaacc 2400
tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga tgagatcgat 2460
ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt ccgtggtatg 2520
atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca tttaattgtc 2580
aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg ttgggctgta 2640
gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc tgtatgtgtc 2700
acatatatct tcatgattaa tatggttgga attatctctt catcttttag atatatatgg 2760
ataggtatat atgttgctgt gggttttact ggtactttat tagatatatt catgcttaga 2820
tacatgaagc aacgtgctgt tacagtttaa taattcttgt ttatctaata aacaaataag 2880
gataggtata tatgttgctg atggttttac tgatacttta ttagatagta cttctttgac 2940
atgaaggaac atcctgcgac agcttaataa ttattcttca tctaataaaa agcttgcttt 3000
ttaattattt tgatatactt ggatgatgtc atgcagcagc tatgtgtgaa ttttcggccc 3060
tgtcttcata tgatgtttat ttgcttggga ctgtttcttt ggctgataac tcaccctgtt 3120
gtttggtgat ccttctgcag gtg 3143
<210> 2
<211> 2005
<212> DNA
<213> Agrostis nebulosa
<400> 2
ggcctcttta cgtttggcac aatttaattg aatcccggca tggcatgtta gaccggagtg 60
agccggccct tttactggta tgacactccc tctgtcttga gtgtcgctgt gccagcttgt 120
acctctgtct atgttcacag cccgtgctgt gtacctagac cctccgtttg tccacattca 180
ttttaatctc tattgtatct tgtcaaaacc taaaagccta aaacgactct gataaaggga 240
cagaaagatt atacaagagc aagtgtataa tgaaataatg taagcgagct atatgaattg 300
tcacgtgtca tatttatgtt gagacgaaga agagaaaata aacaccatgc aaatttatgg 360
cgagtgatag atggccagat gggcacaagg cctcctattt cttaaatcgg attttgtaag 420
aacgaaaaaa gggacttata agagaatagg atagaccata tatcaatgat gtagtatgca 480
tcaagatcta actattatat gagtgaattg ataaatttat tctaggtgac atggccttaa 540
cgatgaacag tacatggtta aatcaataga acaatagcca actctagcag ctctaaaaaa 600
agatatatat tcgtcgaggc actattatgc aaccacatag tcaacttcaa caccgcttga 660
gtgcgttctc atgttttttt tttcttgcaa attacgcttt tctaaaataa aataatttgg 720
atcgtgcaat tatttcactt taggtgtgcg tgactacgtg agtaacattt ttgaatctca 780
gaaaggaaat aaaagtataa tactgctgcc tactttgagg attcggcttg ttatttaaaa 840
ccgtctttaa ggtcaaatgc tcaagattca ttcaacaatt gaaacgtctc acatgattaa 900
atcatgtata aggatgctaa ggtcttgctt gacaatgttt ttctaggaat ttcatctaac 960
tttttgagtg aaactatcaa ataataattt taaaacaatt ttataagaga agctccggag 1020
ataaaaggcc atctaatcta tgttagaaga gtgaagttta ctccctctgt cccaaaaata 1080
gaattctaag tatgaaatga tttttttgtt atacaaaagg agtatatatc acaagattga 1140
tgtcagttat gcttagggca cgtacacgac gctggtgctt taggtagacg ttaatcgttg 1200
tttctgcatt ttattttatt ttgttgccac ggtgtacatt tgggtagacg tttgtcacag 1260
gcattgccac tcaaacaagc agccggcgct tggagctttt atagtttgaa aagtgacggt 1320
tttaaggatg ggtaagctga ttagtatatg taagtttagc tttttccatt gtaggttaag 1380
ccttaaggct cttacacaat tgtttcatta ttctcattct ttaagagccc atataagcgt 1440
tcatgaattg tacatatcct tagatttttt tttttgggta aagctcgagc ttctgtatct 1500
aaaagtagag aaatcagaaa aagattcatg ttttggtagt tttgatttct tgcctccata 1560
ataattttgg tttaccattt tttgtttgat tttagtttta gaagcgttta tagcaggatt 1620
taaaatccaa aactaccatt atcttcaagt gaccgtcagt gagccgttta acggcgtcga 1680
caagtccaac ggacaccaac cagtgaacca ccagcgtcga gccaagcgat gcaaacggaa 1740
cggccgagac gttgacacct ttggcgcggc acggcatgtc ggatctccct ctctggcccc 1800
ctctcgagag ttccagctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt 1860
tccgcctcct gcctgctcct ctcagacggc acgaaaccgt gacggcaccg gcagcacggg 1920
gggattcctt ttccactgct ccttcctttt cccttcctcg cccgccgcta taaatagcca 1980
gccccgtccc cagattcttt cccaa 2005
<210> 3
<211> 85
<212> DNA
<213> Agrostis nebulosa
<400> 3
cctcatcttt gttcggagca cccacacaac ccgatcccca attccctcgt ctctcctcgc 60
gagcctcgtc gaccccccct tcaag 85
<210> 4
<211> 1053
<212> DNA
<213> Agrostis nebulosa
<400> 4
gtacggcgat cgtcctccct ccctctctct ctctaccttc tcttctctag actagatcgg 60
cgacccggtc catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg 120
taaaatagat ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc 180
ctttaggaca tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct 240
aggcagtggg gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa 300
ctgggaaacc tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga 360
tgagatcgat ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt 420
ccgtggtatg atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca 480
tttaattgtc aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg 540
ttgggctgta gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc 600
tgtatgtgtc acatatatct tcatgattaa tatggttgga attatctctt catcttttag 660
atatatatgg ataggtatat atgttgctgt gggttttact ggtactttat tagatatatt 720
catgcttaga tacatgaagc aacgtgctgt tacagtttaa taattcttgt ttatctaata 780
aacaaataag gataggtata tatgttgctg atggttttac tgatacttta ttagatagta 840
cttctttgac atgaaggaac atcctgcgac agcttaataa ttattcttca tctaataaaa 900
agcttgcttt ttaattattt tgatatactt ggatgatgtc atgcagcagc tatgtgtgaa 960
ttttcggccc tgtcttcata tgatgtttat ttgcttggga ctgtttcttt ggctgataac 1020
tcaccctgtt gtttggtgat ccttctgcag gtg 1053
<210> 5
<211> 2137
<212> DNA
<213> Agrostis nebulosa
<400> 5
gagaagctcc ggagataaaa ggccatctaa tctatgttag aagagtgaag tttactccct 60
ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacaa aaggagtata 120
tatcacaaga ttgatgtcag ttatgcttag ggcacgtaca cgacgctggt gctttaggta 180
gacgttaatc gttgtttctg cattttattt tattttgttg ccacggtgta catttgggta 240
gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt 300
tgaaaagtga cggttttaag gatgggtaag ctgattagta tatgtaagtt tagctttttc 360
cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga 420
gcccatataa gcgttcatga attgtacata tccttagatt tttttttttg ggtaaagctc 480
gagcttctgt atctaaaagt agagaaatca gaaaaagatt catgttttgg tagttttgat 540
ttcttgcctc cataataatt ttggtttacc attttttgtt tgattttagt tttagaagcg 600
tttatagcag gatttaaaat ccaaaactac cattatcttc aagtgaccgt cagtgagccg 660
tttaacggcg tcgacaagtc caacggacac caaccagtga accaccagcg tcgagccaag 720
cgatgcaaac ggaacggccg agacgttgac acctttggcg cggcacggca tgtcggatct 780
ccctctctgg ccccctctcg agagttccag ctccacctcc accggtggcg gtttccaagt 840
ccgttccgtt ccgttccgcc tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc 900
accggcagca cggggggatt ccttttccac tgctccttcc ttttcccttc ctcgcccgcc 960
gctataaata gccagccccg tccccagatt ctttcccaac ctcatctttg ttcggagcac 1020
ccacacaacc cgatccccaa ttccctcgtc tctcctcgcg agcctcgtcg accccccctt 1080
caaggtacgg cgatcgtcct ccctccctct ctctctctac cttctcttct ctagactaga 1140
tcggcgaccc ggtccatggt tagggcctgc tagttctgtt cctgtttttt ccatggctgc 1200
gaggtaaaat agatctgatg gcgttatgat ggttaactcg tcatactctt gcgatctatg 1260
gtccctttag gacatcgatt taatttcgga tggttcgaga tcggtgatcc atggttagta 1320
ccctaggcag tggggttaga tccgtgctgt tagggttcgt agatggattc tgattgctca 1380
gtaactggga aacctgggat ggttctagct gggaatcctg ggatggttct agctggttcg 1440
cagatgagat cgatttcatg gtctgctata tcttgtttcg ttgcctaggt tccgtttaat 1500
ctgtccgtgg tatgatgtta gcctttgata aggttcgatc gtgctagcta cgtcctgcgc 1560
agcatttaat tgtcaggtca taatttttag cattcctgtt tttgtttggt ttggttttgt 1620
ctggttgggc tgtagatagt ttcaatctac ctgtcggttt attttattaa atttggattg 1680
gatctgtatg tgtcacatat atcttcatga ttaatatggt tggaattatc tcttcatctt 1740
ttagatatat atggataggt atatatgttg ctgtgggttt tactggtact ttattagata 1800
tattcatgct tagatacatg aagcaacgtg ctgttacagt ttaataattc ttgtttatct 1860
aataaacaaa taaggatagg tatatatgtt gctgatggtt ttactgatac tttattagat 1920
agtacttctt tgacatgaag gaacatcctg cgacagctta ataattattc ttcatctaat 1980
aaaaagcttg ctttttaatt attttgatat acttggatga tgtcatgcag cagctatgtg 2040
tgaattttcg gccctgtctt catatgatgt ttatttgctt gggactgttt ctttggctga 2100
taactcaccc tgttgtttgg tgatccttct gcaggtg 2137
<210> 6
<211> 999
<212> DNA
<213> Agrostis nebulosa
<400> 6
gagaagctcc ggagataaaa ggccatctaa tctatgttag aagagtgaag tttactccct 60
ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacaa aaggagtata 120
tatcacaaga ttgatgtcag ttatgcttag ggcacgtaca cgacgctggt gctttaggta 180
gacgttaatc gttgtttctg cattttattt tattttgttg ccacggtgta catttgggta 240
gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt 300
tgaaaagtga cggttttaag gatgggtaag ctgattagta tatgtaagtt tagctttttc 360
cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga 420
gcccatataa gcgttcatga attgtacata tccttagatt tttttttttg ggtaaagctc 480
gagcttctgt atctaaaagt agagaaatca gaaaaagatt catgttttgg tagttttgat 540
ttcttgcctc cataataatt ttggtttacc attttttgtt tgattttagt tttagaagcg 600
tttatagcag gatttaaaat ccaaaactac cattatcttc aagtgaccgt cagtgagccg 660
tttaacggcg tcgacaagtc caacggacac caaccagtga accaccagcg tcgagccaag 720
cgatgcaaac ggaacggccg agacgttgac acctttggcg cggcacggca tgtcggatct 780
ccctctctgg ccccctctcg agagttccag ctccacctcc accggtggcg gtttccaagt 840
ccgttccgtt ccgttccgcc tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc 900
accggcagca cggggggatt ccttttccac tgctccttcc ttttcccttc ctcgcccgcc 960
gctataaata gccagccccg tccccagatt ctttcccaa 999
<210> 7
<211> 1900
<212> DNA
<213> Agrostis nebulosa
<400> 7
gtagacgttt gtcacaggca ttgccactca aacaagcagc cggcgcttgg agcttttata 60
gtttgaaaag tgacggtttt aaggatgggt aagctgatta gtatatgtaa gtttagcttt 120
ttccattgta ggttaagcct taaggctctt acacaattgt ttcattattc tcattcttta 180
agagcccata taagcgttca tgaattgtac atatccttag attttttttt ttgggtaaag 240
ctcgagcttc tgtatctaaa agtagagaaa tcagaaaaag attcatgttt tggtagtttt 300
gatttcttgc ctccataata attttggttt accatttttt gtttgatttt agttttagaa 360
gcgtttatag caggatttaa aatccaaaac taccattatc ttcaagtgac cgtcagtgag 420
ccgtttaacg gcgtcgacaa gtccaacgga caccaaccag tgaaccacca gcgtcgagcc 480
aagcgatgca aacggaacgg ccgagacgtt gacacctttg gcgcggcacg gcatgtcgga 540
tctccctctc tggccccctc tcgagagttc cagctccacc tccaccggtg gcggtttcca 600
agtccgttcc gttccgttcc gcctcctgcc tgctcctctc agacggcacg aaaccgtgac 660
ggcaccggca gcacgggggg attccttttc cactgctcct tccttttccc ttcctcgccc 720
gccgctataa atagccagcc ccgtccccag attctttccc aacctcatct ttgttcggag 780
cacccacaca acccgatccc caattccctc gtctctcctc gcgagcctcg tcgacccccc 840
cttcaaggta cggcgatcgt cctccctccc tctctctctc taccttctct tctctagact 900
agatcggcga cccggtccat ggttagggcc tgctagttct gttcctgttt tttccatggc 960
tgcgaggtaa aatagatctg atggcgttat gatggttaac tcgtcatact cttgcgatct 1020
atggtccctt taggacatcg atttaatttc ggatggttcg agatcggtga tccatggtta 1080
gtaccctagg cagtggggtt agatccgtgc tgttagggtt cgtagatgga ttctgattgc 1140
tcagtaactg ggaaacctgg gatggttcta gctgggaatc ctgggatggt tctagctggt 1200
tcgcagatga gatcgatttc atggtctgct atatcttgtt tcgttgccta ggttccgttt 1260
aatctgtccg tggtatgatg ttagcctttg ataaggttcg atcgtgctag ctacgtcctg 1320
cgcagcattt aattgtcagg tcataatttt tagcattcct gtttttgttt ggtttggttt 1380
tgtctggttg ggctgtagat agtttcaatc tacctgtcgg tttattttat taaatttgga 1440
ttggatctgt atgtgtcaca tatatcttca tgattaatat ggttggaatt atctcttcat 1500
cttttagata tatatggata ggtatatatg ttgctgtggg ttttactggt actttattag 1560
atatattcat gcttagatac atgaagcaac gtgctgttac agtttaataa ttcttgttta 1620
tctaataaac aaataaggat aggtatatat gttgctgatg gttttactga tactttatta 1680
gatagtactt ctttgacatg aaggaacatc ctgcgacagc ttaataatta ttcttcatct 1740
aataaaaagc ttgcttttta attattttga tatacttgga tgatgtcatg cagcagctat 1800
gtgtgaattt tcggccctgt cttcatatga tgtttatttg cttgggactg tttctttggc 1860
tgataactca ccctgttgtt tggtgatcct tctgcaggtg 1900
<210> 8
<211> 762
<212> DNA
<213> Agrostis nebulosa
<400> 8
gtagacgttt gtcacaggca ttgccactca aacaagcagc cggcgcttgg agcttttata 60
gtttgaaaag tgacggtttt aaggatgggt aagctgatta gtatatgtaa gtttagcttt 120
ttccattgta ggttaagcct taaggctctt acacaattgt ttcattattc tcattcttta 180
agagcccata taagcgttca tgaattgtac atatccttag attttttttt ttgggtaaag 240
ctcgagcttc tgtatctaaa agtagagaaa tcagaaaaag attcatgttt tggtagtttt 300
gatttcttgc ctccataata attttggttt accatttttt gtttgatttt agttttagaa 360
gcgtttatag caggatttaa aatccaaaac taccattatc ttcaagtgac cgtcagtgag 420
ccgtttaacg gcgtcgacaa gtccaacgga caccaaccag tgaaccacca gcgtcgagcc 480
aagcgatgca aacggaacgg ccgagacgtt gacacctttg gcgcggcacg gcatgtcgga 540
tctccctctc tggccccctc tcgagagttc cagctccacc tccaccggtg gcggtttcca 600
agtccgttcc gttccgttcc gcctcctgcc tgctcctctc agacggcacg aaaccgtgac 660
ggcaccggca gcacgggggg attccttttc cactgctcct tccttttccc ttcctcgccc 720
gccgctataa atagccagcc ccgtccccag attctttccc aa 762
<210> 9
<211> 5068
<212> DNA
<213> Arundo donax
<400> 9
ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttaa catttgaaga 60
ttgaaccggg cactaatgca agtctacaac taagaactac aagaaagcat gttccttgag 120
gtacttggat gcaacctcac aattatcaaa ttaattaaca actacagtta gaattttaga 180
tcacaagaat atcacgaact gtggatacta cttcaagggc tattcttttc tgaatgttgc 240
agttggttgt tttaaacata ttacaaacta ggtgtttaaa tgccaaaaag ttcatggaaa 300
aagattaagc taatattcca tccgtccaca aaatttaaat gctaggaatc attatatttg 360
tggatagaag gagtagttag taagacctac acttaattac acattggtca ttcctggagg 420
aataatcccc catagcaagt tgttttgagt ttgactaccc aaacttgcat aaattttttc 480
ttaaaaaaag ggggagcttc accattccat caagatggcg aggctaaatg aaacgcacga 540
tgggcaaaac ggactaacgt acaaacaaca aggcaatgaa agatagggtt ttgataaata 600
tcaaatatac aaagtcaacc aaagaaaaaa gagatcccaa tggctaacct ttggatccgt 660
gtcgcaattt gtgctttagg acatacaagg tggatttctt ctttggcaaa ctctataata 720
attgggtgac ggtggcctca cggcagcctc aaagagtcgg tagcaacttt tagatctttt 780
gagctgaaac tcaattatgt agtagaatga tatttagata gatagatcga aatttggggg 840
tgtgaaaaca aagaggttct caatattgat agcaactcca acgaatggat atggaaaata 900
catgattttt tattcgagta gaaaaaggag gggaacggaa caaatctagc aatagtagcc 960
accaaagatg aggaccctgg atttcggatc caatagtggt aaggaagaaa gggccggact 1020
atccagaata aggtgaattg gtcaaggaag cggaagtctc cataaagaaa ttgtgggctc 1080
acttgatgtg agaaagaaga ccgaccaaga agcgggtttt gggggacaga ggagattggt 1140
gccaggttgc agtggcatgt atgtggggga agaggcaatg acaacggccg agagaggaga 1200
agggaatgag gtaagtattt gaagtgaaga ggtgcccata taggttaaaa aatgagctgt 1260
ggatttaaat caaaggtgtc agcgacacag gcacggaagt accctaagtt acctatgtgg 1320
gtcgcatatc acgctaagtt ccttcaccgt acaaggtgaa agtaacactg gcaatgtgcc 1380
ccagctgcaa ggcttgtcta tcaatgtggc cctacaaggc tcctggctac cccaggagct 1440
caaaacacgt ggcacatggt ggtacttcgc ccgacctcta tgctcaccgt gcacccggcc 1500
ccgaggtcaa tggctcctga gcacccgact gcatgactgg acccctgagt acccgacccc 1560
cgggacaagc tcccgtggac cttccccggg gatcaggctc acgagtactc gacctcacgt 1620
caatggctct cccgagtacc caaccttgtg tcgatagctc actaaggatc atgtgctaat 1680
ccttagcatc tcggattttg agtactaggc cattatttgc atgccatcct cttggatcta 1740
tgcggatttt caaggacctt acctaagcat caacatgcac aaacacaaac ccttcgtgaa 1800
gccatcccca actactcggg tggcaggacc ctcgacacgt gcgatgcgag ctcggacaga 1860
gctgacaaga acctcccgac ggcgcattaa atgccctggc aagggcgccc cgcctcgtcg 1920
agctctggac ttcatcaagt cacatcaaca gcaggcaggc gctccttccg cagacttcat 1980
catgagggaa tccgttaccc tctatttaca tagtgcagcg gggaatgtgg agatcaaatc 2040
tctccaatga tgtcactgtg tagcatgtat tagcacgcca acaccctgtc gcttaccacg 2100
aggatcagcc atgcaagcaa gagatgttgg tcgggcctcg gtggcaactg aggctatagt 2160
gacctatgac gagcaggcca tagataggcc cactggcaag cccaagaatc gctagacggg 2220
ctagatctgg acacttgtcc gcaccaagca ctaccgttgc aactgcaacc tctatatgta 2280
actatagatt cacatgttgc gacatctttg cccaatacgt attgtaccct agacagctca 2340
ccctatcttt ttcttttttt tcctctttct tcttcctcct ccttgcatgg agacgtagaa 2400
ggactcctcc cttgtgacta ttaaaggaag gacttagggc tgtgctaggg gagagaactt 2460
ttggacttgg gagagctctg cactgaacat cttcctctcc acgcttgtaa tattttccac 2520
aacaaagaat tccataaagc cggatgtagg gctattatcc ctctcgggag gcctgaacca 2580
gggtaaaaca ccactcttct caccagcgtt cgccgcatta gtctagacta gcatcttttg 2640
accctatatc gaaccatcta gggactttac gtcccctgcc tgcagtttcc cggtgacaga 2700
atgactatga tttttcgtcg attttataaa agtgaaaaca accggttgat atctatgcgc 2760
actattttcc tacatatatt tctaacttct tgcttagcca tgtcggttaa gagcaagtgg 2820
agagcactct catttcgtag aacaagtgat gaatgccgac ctgcatcatc ttacttagac 2880
ttgatcatca agtggaatcc ccattcatct taataatctc atattgagtg ccaatgcaac 2940
attgttataa tcctcttcat atgctaattc ttcaaagcta acgtagttaa atgaaggcaa 3000
aatatgcaac ttcgtcctct aagtttgctc aaaggctcat ttttaccctt taactatcaa 3060
accgattact ttcgtccctg aactttcatg tttggtccaa tttaatccct gggctgatgt 3120
atccgtccac ggtggtgtgt ccaatcagtg aataatctag ttagtgaagc cagaagtcca 3180
tagtgcccct tgctctgtca ccatatatcc agttcaaccg caccaatttg ccatctcgaa 3240
ctggttcatg ttttattcag gttggtaaat gaattttgcc aattcaatgt agttagatat 3300
ttccatgtca ttttagtaca tttaccaatt ttttatattc tggctagaaa aggagaatgg 3360
tgacgtcttt cggaagatca agatcaatta tcaagtatca gcaacagcac ctgaaggttg 3420
gagtgcatta gttgtcattg agaataatgc tagctattca ttgcactggc attagagaca 3480
gagagggcga gccagtttga catggcaaat tagcacagtc aaactggata cgtggtgacg 3540
gagggagggg cactatgaat ttttggtgac ggagggaggg gcactatgaa tttttggctt 3600
tgctgacggg acacgccact atggatgaaa ttggacaaaa tacgaatatt caaggatgaa 3660
agtggtcggt ttgatagttc agggatgaaa tgtgtctttg ggcaaacttt gaggacgaag 3720
ttgcctattt tgcattaaac gaatatattt atatacccca aaaaaaagaa tacacatctc 3780
cactccgagc cggcatgtgg ggtccccact agtcagccac tgtatggcgc cgactagctc 3840
aacggccacg aaccagccaa ccaccagcgc aacctaaacg gcgtaaacgt tgacggcatc 3900
tctctctcgc cccgtctcga agcttccgca ccgctcgctg gtcgctgccc ggcgccgctc 3960
gtgctggact ctttccgtgg cggcttccgc gaaattgcgt ggtggagagg agagacggaa 4020
ccgtcacggc actggattcc ttccccaccc ggcttggccg gcccctcctc gcctccataa 4080
ataggcaccc cgtcctcgcc tcctctcccc acctcatctc ctcctttccc gtgaaccgtg 4140
aacacaaccc gacccagatc ccctcttgcg agcttcgtcg atccctcctc cgcgtcaagg 4200
tacggagctt ctcctccccc ttcttctcta gatcggcgtg ttatgttgtt tccgtggttg 4260
cttggttgga tgaatcgaat gattcttagg gcctaggagg ctggttagat ctgttgcgtt 4320
ctgtttcgta gatggatttt ggtgtaagat caggtcggtt ccgctgttta acttgtgatg 4380
ctagtgtgat ttttgggagg atttgagttg ttaatctggg agttgttggg aggttctcgt 4440
aggcggattg tagatgaagt cgcccgcacg atttgcgtgg cttgttgggt agctagggtt 4500
agatctgctc ggatttttca ttgttactta ttgagagata atgtagctaa cctttacttg 4560
ttcatctatg tatctcgtat tcgtattcat ctggttcgat ggtgctagat agatgcgcct 4620
gatttgtccg atcgaattgg gtagcatccg cggcttgttt ggtagtgttc tgattgattt 4680
gtcgctctag atctgagtgg aataatatta catctcaaca tgttactaga aacttggttt 4740
atagctccgg atttacatgt ttattcttat gtaaggtttt aaatgaaaga tttatgctac 4800
tgctgctcgt tgatccttta gcatccacct gaggaacatg catgcatctg ttacttcttt 4860
tgatatatgc ttagatagtt gttagtatat actgctgttg ttcgatgatc cttcaggatg 4920
aacatgcatg atcatgttac ttgtttttat atgcttctgc tgttcgttga ttctttagta 4980
ctacctacct gatcatcttg catgtttcct gcttgttaga gattaattga ttaggcttac 5040
cttgttgcct ggtgattctt ccttgcag 5068
<210> 10
<211> 4114
<212> DNA
<213> Arundo donax
<400> 10
ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttaa catttgaaga 60
ttgaaccggg cactaatgca agtctacaac taagaactac aagaaagcat gttccttgag 120
gtacttggat gcaacctcac aattatcaaa ttaattaaca actacagtta gaattttaga 180
tcacaagaat atcacgaact gtggatacta cttcaagggc tattcttttc tgaatgttgc 240
agttggttgt tttaaacata ttacaaacta ggtgtttaaa tgccaaaaag ttcatggaaa 300
aagattaagc taatattcca tccgtccaca aaatttaaat gctaggaatc attatatttg 360
tggatagaag gagtagttag taagacctac acttaattac acattggtca ttcctggagg 420
aataatcccc catagcaagt tgttttgagt ttgactaccc aaacttgcat aaattttttc 480
ttaaaaaaag ggggagcttc accattccat caagatggcg aggctaaatg aaacgcacga 540
tgggcaaaac ggactaacgt acaaacaaca aggcaatgaa agatagggtt ttgataaata 600
tcaaatatac aaagtcaacc aaagaaaaaa gagatcccaa tggctaacct ttggatccgt 660
gtcgcaattt gtgctttagg acatacaagg tggatttctt ctttggcaaa ctctataata 720
attgggtgac ggtggcctca cggcagcctc aaagagtcgg tagcaacttt tagatctttt 780
gagctgaaac tcaattatgt agtagaatga tatttagata gatagatcga aatttggggg 840
tgtgaaaaca aagaggttct caatattgat agcaactcca acgaatggat atggaaaata 900
catgattttt tattcgagta gaaaaaggag gggaacggaa caaatctagc aatagtagcc 960
accaaagatg aggaccctgg atttcggatc caatagtggt aaggaagaaa gggccggact 1020
atccagaata aggtgaattg gtcaaggaag cggaagtctc cataaagaaa ttgtgggctc 1080
acttgatgtg agaaagaaga ccgaccaaga agcgggtttt gggggacaga ggagattggt 1140
gccaggttgc agtggcatgt atgtggggga agaggcaatg acaacggccg agagaggaga 1200
agggaatgag gtaagtattt gaagtgaaga ggtgcccata taggttaaaa aatgagctgt 1260
ggatttaaat caaaggtgtc agcgacacag gcacggaagt accctaagtt acctatgtgg 1320
gtcgcatatc acgctaagtt ccttcaccgt acaaggtgaa agtaacactg gcaatgtgcc 1380
ccagctgcaa ggcttgtcta tcaatgtggc cctacaaggc tcctggctac cccaggagct 1440
caaaacacgt ggcacatggt ggtacttcgc ccgacctcta tgctcaccgt gcacccggcc 1500
ccgaggtcaa tggctcctga gcacccgact gcatgactgg acccctgagt acccgacccc 1560
cgggacaagc tcccgtggac cttccccggg gatcaggctc acgagtactc gacctcacgt 1620
caatggctct cccgagtacc caaccttgtg tcgatagctc actaaggatc atgtgctaat 1680
ccttagcatc tcggattttg agtactaggc cattatttgc atgccatcct cttggatcta 1740
tgcggatttt caaggacctt acctaagcat caacatgcac aaacacaaac ccttcgtgaa 1800
gccatcccca actactcggg tggcaggacc ctcgacacgt gcgatgcgag ctcggacaga 1860
gctgacaaga acctcccgac ggcgcattaa atgccctggc aagggcgccc cgcctcgtcg 1920
agctctggac ttcatcaagt cacatcaaca gcaggcaggc gctccttccg cagacttcat 1980
catgagggaa tccgttaccc tctatttaca tagtgcagcg gggaatgtgg agatcaaatc 2040
tctccaatga tgtcactgtg tagcatgtat tagcacgcca acaccctgtc gcttaccacg 2100
aggatcagcc atgcaagcaa gagatgttgg tcgggcctcg gtggcaactg aggctatagt 2160
gacctatgac gagcaggcca tagataggcc cactggcaag cccaagaatc gctagacggg 2220
ctagatctgg acacttgtcc gcaccaagca ctaccgttgc aactgcaacc tctatatgta 2280
actatagatt cacatgttgc gacatctttg cccaatacgt attgtaccct agacagctca 2340
ccctatcttt ttcttttttt tcctctttct tcttcctcct ccttgcatgg agacgtagaa 2400
ggactcctcc cttgtgacta ttaaaggaag gacttagggc tgtgctaggg gagagaactt 2460
ttggacttgg gagagctctg cactgaacat cttcctctcc acgcttgtaa tattttccac 2520
aacaaagaat tccataaagc cggatgtagg gctattatcc ctctcgggag gcctgaacca 2580
gggtaaaaca ccactcttct caccagcgtt cgccgcatta gtctagacta gcatcttttg 2640
accctatatc gaaccatcta gggactttac gtcccctgcc tgcagtttcc cggtgacaga 2700
atgactatga tttttcgtcg attttataaa agtgaaaaca accggttgat atctatgcgc 2760
actattttcc tacatatatt tctaacttct tgcttagcca tgtcggttaa gagcaagtgg 2820
agagcactct catttcgtag aacaagtgat gaatgccgac ctgcatcatc ttacttagac 2880
ttgatcatca agtggaatcc ccattcatct taataatctc atattgagtg ccaatgcaac 2940
attgttataa tcctcttcat atgctaattc ttcaaagcta acgtagttaa atgaaggcaa 3000
aatatgcaac ttcgtcctct aagtttgctc aaaggctcat ttttaccctt taactatcaa 3060
accgattact ttcgtccctg aactttcatg tttggtccaa tttaatccct gggctgatgt 3120
atccgtccac ggtggtgtgt ccaatcagtg aataatctag ttagtgaagc cagaagtcca 3180
tagtgcccct tgctctgtca ccatatatcc agttcaaccg caccaatttg ccatctcgaa 3240
ctggttcatg ttttattcag gttggtaaat gaattttgcc aattcaatgt agttagatat 3300
ttccatgtca ttttagtaca tttaccaatt ttttatattc tggctagaaa aggagaatgg 3360
tgacgtcttt cggaagatca agatcaatta tcaagtatca gcaacagcac ctgaaggttg 3420
gagtgcatta gttgtcattg agaataatgc tagctattca ttgcactggc attagagaca 3480
gagagggcga gccagtttga catggcaaat tagcacagtc aaactggata cgtggtgacg 3540
gagggagggg cactatgaat ttttggtgac ggagggaggg gcactatgaa tttttggctt 3600
tgctgacggg acacgccact atggatgaaa ttggacaaaa tacgaatatt caaggatgaa 3660
agtggtcggt ttgatagttc agggatgaaa tgtgtctttg ggcaaacttt gaggacgaag 3720
ttgcctattt tgcattaaac gaatatattt atatacccca aaaaaaagaa tacacatctc 3780
cactccgagc cggcatgtgg ggtccccact agtcagccac tgtatggcgc cgactagctc 3840
aacggccacg aaccagccaa ccaccagcgc aacctaaacg gcgtaaacgt tgacggcatc 3900
tctctctcgc cccgtctcga agcttccgca ccgctcgctg gtcgctgccc ggcgccgctc 3960
gtgctggact ctttccgtgg cggcttccgc gaaattgcgt ggtggagagg agagacggaa 4020
ccgtcacggc actggattcc ttccccaccc ggcttggccg gcccctcctc gcctccataa 4080
ataggcaccc cgtcctcgcc tcctctcccc acct 4114
<210> 11
<211> 85
<212> DNA
<213> Arundo donax
<400> 11
catctcctcc tttcccgtga accgtgaaca caacccgacc cagatcccct cttgcgagct 60
tcgtcgatcc ctcctccgcg tcaag 85
<210> 12
<211> 869
<212> DNA
<213> Arundo donax
<400> 12
gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt 60
gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt 120
tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat 180
gctagtgtga tttttgggag gatttgagtt gttaatctgg gagttgttgg gaggttctcg 240
taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt 300
tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt 360
gttcatctat gtatctcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc 420
tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt 480
tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt 540
tatagctccg gatttacatg tttattctta tgtaaggttt taaatgaaag atttatgcta 600
ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt 660
ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat 720
gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt 780
actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta 840
ccttgttgcc tggtgattct tccttgcag 869
<210> 13
<211> 2969
<212> DNA
<213> Arundo donax
<400> 13
gatcagccat gcaagcaaga gatgttggtc gggcctcggt ggcaactgag gctatagtga 60
cctatgacga gcaggccata gataggccca ctggcaagcc caagaatcgc tagacgggct 120
agatctggac acttgtccgc accaagcact accgttgcaa ctgcaacctc tatatgtaac 180
tatagattca catgttgcga catctttgcc caatacgtat tgtaccctag acagctcacc 240
ctatcttttt cttttttttc ctctttcttc ttcctcctcc ttgcatggag acgtagaagg 300
actcctccct tgtgactatt aaaggaagga cttagggctg tgctagggga gagaactttt 360
ggacttggga gagctctgca ctgaacatct tcctctccac gcttgtaata ttttccacaa 420
caaagaattc cataaagccg gatgtagggc tattatccct ctcgggaggc ctgaaccagg 480
gtaaaacacc actcttctca ccagcgttcg ccgcattagt ctagactagc atcttttgac 540
cctatatcga accatctagg gactttacgt cccctgcctg cagtttcccg gtgacagaat 600
gactatgatt tttcgtcgat tttataaaag tgaaaacaac cggttgatat ctatgcgcac 660
tattttccta catatatttc taacttcttg cttagccatg tcggttaaga gcaagtggag 720
agcactctca tttcgtagaa caagtgatga atgccgacct gcatcatctt acttagactt 780
gatcatcaag tggaatcccc attcatctta ataatctcat attgagtgcc aatgcaacat 840
tgttataatc ctcttcatat gctaattctt caaagctaac gtagttaaat gaaggcaaaa 900
tatgcaactt cgtcctctaa gtttgctcaa aggctcattt ttacccttta actatcaaac 960
cgattacttt cgtccctgaa ctttcatgtt tggtccaatt taatccctgg gctgatgtat 1020
ccgtccacgg tggtgtgtcc aatcagtgaa taatctagtt agtgaagcca gaagtccata 1080
gtgccccttg ctctgtcacc atatatccag ttcaaccgca ccaatttgcc atctcgaact 1140
ggttcatgtt ttattcaggt tggtaaatga attttgccaa ttcaatgtag ttagatattt 1200
ccatgtcatt ttagtacatt taccaatttt ttatattctg gctagaaaag gagaatggtg 1260
acgtctttcg gaagatcaag atcaattatc aagtatcagc aacagcacct gaaggttgga 1320
gtgcattagt tgtcattgag aataatgcta gctattcatt gcactggcat tagagacaga 1380
gagggcgagc cagtttgaca tggcaaatta gcacagtcaa actggatacg tggtgacgga 1440
gggaggggca ctatgaattt ttggtgacgg agggaggggc actatgaatt tttggctttg 1500
ctgacgggac acgccactat ggatgaaatt ggacaaaata cgaatattca aggatgaaag 1560
tggtcggttt gatagttcag ggatgaaatg tgtctttggg caaactttga ggacgaagtt 1620
gcctattttg cattaaacga atatatttat ataccccaaa aaaaagaata cacatctcca 1680
ctccgagccg gcatgtgggg tccccactag tcagccactg tatggcgccg actagctcaa 1740
cggccacgaa ccagccaacc accagcgcaa cctaaacggc gtaaacgttg acggcatctc 1800
tctctcgccc cgtctcgaag cttccgcacc gctcgctggt cgctgcccgg cgccgctcgt 1860
gctggactct ttccgtggcg gcttccgcga aattgcgtgg tggagaggag agacggaacc 1920
gtcacggcac tggattcctt ccccacccgg cttggccggc ccctcctcgc ctccataaat 1980
aggcaccccg tcctcgcctc ctctccccac ctcatctcct cctttcccgt gaaccgtgaa 2040
cacaacccga cccagatccc ctcttgcgag cttcgtcgat ccctcctccg cgtcaaggta 2100
cggagcttct cctccccctt cttctctaga tcggcgtgtt atgttgtttc cgtggttgct 2160
tggttggatg aatcgaatga ttcttagggc ctaggaggct ggttagatct gttgcgttct 2220
gtttcgtaga tggattttgg tgtaagatca ggtcggttcc gctgtttaac ttgtgatgct 2280
agtgtgattt ttgggaggat ttgagttgtt aatctgggag ttgttgggag gttctcgtag 2340
gcggattgta gatgaagtcg cccgcacgat ttgcgtggct tgttgggtag ctagggttag 2400
atctgctcgg atttttcatt gttacttatt gagagataat gtagctaacc tttacttgtt 2460
catctatgta tctcgtattc gtattcatct ggttcgatgg tgctagatag atgcgcctga 2520
tttgtccgat cgaattgggt agcatccgcg gcttgtttgg tagtgttctg attgatttgt 2580
cgctctagat ctgagtggaa taatattaca tctcaacatg ttactagaaa cttggtttat 2640
agctccggat ttacatgttt attcttatgt aaggttttaa atgaaagatt tatgctactg 2700
ctgctcgttg atcctttagc atccacctga ggaacatgca tgcatctgtt acttcttttg 2760
atatatgctt agatagttgt tagtatatac tgctgttgtt cgatgatcct tcaggatgaa 2820
catgcatgat catgttactt gtttttatat gcttctgctg ttcgttgatt ctttagtact 2880
acctacctga tcatcttgca tgtttcctgc ttgttagaga ttaattgatt aggcttacct 2940
tgttgcctgg tgattcttcc ttgcaggtg 2969
<210> 14
<211> 2012
<212> DNA
<213> Arundo donax
<400> 14
gatcagccat gcaagcaaga gatgttggtc gggcctcggt ggcaactgag gctatagtga 60
cctatgacga gcaggccata gataggccca ctggcaagcc caagaatcgc tagacgggct 120
agatctggac acttgtccgc accaagcact accgttgcaa ctgcaacctc tatatgtaac 180
tatagattca catgttgcga catctttgcc caatacgtat tgtaccctag acagctcacc 240
ctatcttttt cttttttttc ctctttcttc ttcctcctcc ttgcatggag acgtagaagg 300
actcctccct tgtgactatt aaaggaagga cttagggctg tgctagggga gagaactttt 360
ggacttggga gagctctgca ctgaacatct tcctctccac gcttgtaata ttttccacaa 420
caaagaattc cataaagccg gatgtagggc tattatccct ctcgggaggc ctgaaccagg 480
gtaaaacacc actcttctca ccagcgttcg ccgcattagt ctagactagc atcttttgac 540
cctatatcga accatctagg gactttacgt cccctgcctg cagtttcccg gtgacagaat 600
gactatgatt tttcgtcgat tttataaaag tgaaaacaac cggttgatat ctatgcgcac 660
tattttccta catatatttc taacttcttg cttagccatg tcggttaaga gcaagtggag 720
agcactctca tttcgtagaa caagtgatga atgccgacct gcatcatctt acttagactt 780
gatcatcaag tggaatcccc attcatctta ataatctcat attgagtgcc aatgcaacat 840
tgttataatc ctcttcatat gctaattctt caaagctaac gtagttaaat gaaggcaaaa 900
tatgcaactt cgtcctctaa gtttgctcaa aggctcattt ttacccttta actatcaaac 960
cgattacttt cgtccctgaa ctttcatgtt tggtccaatt taatccctgg gctgatgtat 1020
ccgtccacgg tggtgtgtcc aatcagtgaa taatctagtt agtgaagcca gaagtccata 1080
gtgccccttg ctctgtcacc atatatccag ttcaaccgca ccaatttgcc atctcgaact 1140
ggttcatgtt ttattcaggt tggtaaatga attttgccaa ttcaatgtag ttagatattt 1200
ccatgtcatt ttagtacatt taccaatttt ttatattctg gctagaaaag gagaatggtg 1260
acgtctttcg gaagatcaag atcaattatc aagtatcagc aacagcacct gaaggttgga 1320
gtgcattagt tgtcattgag aataatgcta gctattcatt gcactggcat tagagacaga 1380
gagggcgagc cagtttgaca tggcaaatta gcacagtcaa actggatacg tggtgacgga 1440
gggaggggca ctatgaattt ttggtgacgg agggaggggc actatgaatt tttggctttg 1500
ctgacgggac acgccactat ggatgaaatt ggacaaaata cgaatattca aggatgaaag 1560
tggtcggttt gatagttcag ggatgaaatg tgtctttggg caaactttga ggacgaagtt 1620
gcctattttg cattaaacga atatatttat ataccccaaa aaaaagaata cacatctcca 1680
ctccgagccg gcatgtgggg tccccactag tcagccactg tatggcgccg actagctcaa 1740
cggccacgaa ccagccaacc accagcgcaa cctaaacggc gtaaacgttg acggcatctc 1800
tctctcgccc cgtctcgaag cttccgcacc gctcgctggt cgctgcccgg cgccgctcgt 1860
gctggactct ttccgtggcg gcttccgcga aattgcgtgg tggagaggag agacggaacc 1920
gtcacggcac tggattcctt ccccacccgg cttggccggc ccctcctcgc ctccataaat 1980
aggcaccccg tcctcgcctc ctctccccac ct 2012
<210> 15
<211> 872
<212> DNA
<213> Arundo donax
<400> 15
gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt 60
gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt 120
tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat 180
gctagtgtga tttttgggag gatttgagtt gttaatctgg gagttgttgg gaggttctcg 240
taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt 300
tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt 360
gttcatctat gtatctcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc 420
tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt 480
tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt 540
tatagctccg gatttacatg tttattctta tgtaaggttt taaatgaaag atttatgcta 600
ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt 660
ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat 720
gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt 780
actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta 840
ccttgttgcc tggtgattct tccttgcagg tg 872
<210> 16
<211> 1954
<212> DNA
<213> Arundo donax
<400> 16
tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga 60
agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat 120
ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt 180
agatatttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga 240
gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga 300
aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta 360
gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg 420
gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaatttt 480
tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag 540
gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg 600
acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca 660
catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac 720
tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac 780
ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg 840
ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag 900
acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct 960
ccataaatag gcaccccgtc ctcgcctcct ctccccacct catctcctcc tttcccgtga 1020
accgtgaaca caacccgacc cagatcccct cttgcgagct tcgtcgatcc ctcctccgcg 1080
tcaaggtacg gagcttctcc tcccccttct tctctagatc ggcgtgttat gttgtttccg 1140
tggttgcttg gttggatgaa tcgaatgatt cttagggcct aggaggctgg ttagatctgt 1200
tgcgttctgt ttcgtagatg gattttggtg taagatcagg tcggttccgc tgtttaactt 1260
gtgatgctag tgtgattttt gggaggattt gagttgttaa tctgggagtt gttgggaggt 1320
tctcgtaggc ggattgtaga tgaagtcgcc cgcacgattt gcgtggcttg ttgggtagct 1380
agggttagat ctgctcggat ttttcattgt tacttattga gagataatgt agctaacctt 1440
tacttgttca tctatgtatc tcgtattcgt attcatctgg ttcgatggtg ctagatagat 1500
gcgcctgatt tgtccgatcg aattgggtag catccgcggc ttgtttggta gtgttctgat 1560
tgatttgtcg ctctagatct gagtggaata atattacatc tcaacatgtt actagaaact 1620
tggtttatag ctccggattt acatgtttat tcttatgtaa ggttttaaat gaaagattta 1680
tgctactgct gctcgttgat cctttagcat ccacctgagg aacatgcatg catctgttac 1740
ttcttttgat atatgcttag atagttgtta gtatatactg ctgttgttcg atgatccttc 1800
aggatgaaca tgcatgatca tgttacttgt ttttatatgc ttctgctgtt cgttgattct 1860
ttagtactac ctacctgatc atcttgcatg tttcctgctt gttagagatt aattgattag 1920
gcttaccttg ttgcctggtg attcttcctt gcag 1954
<210> 17
<211> 1000
<212> DNA
<213> Arundo donax
<400> 17
tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga 60
agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat 120
ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt 180
agatatttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga 240
gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga 300
aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta 360
gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg 420
gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaatttt 480
tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag 540
gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg 600
acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca 660
catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac 720
tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac 780
ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg 840
ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag 900
acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct 960
ccataaatag gcaccccgtc ctcgcctcct ctccccacct 1000
<210> 18
<211> 1957
<212> DNA
<213> Arundo donax
<400> 18
tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga 60
agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat 120
ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt 180
agatatttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga 240
gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga 300
aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta 360
gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg 420
gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaatttt 480
tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag 540
gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg 600
acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca 660
catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac 720
tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac 780
ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg 840
ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag 900
acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct 960
ccataaatag gcaccccgtc ctcgcctcct ctccccacct catctcctcc tttcccgtga 1020
accgtgaaca caacccgacc cagatcccct cttgcgagct tcgtcgatcc ctcctccgcg 1080
tcaaggtacg gagcttctcc tcccccttct tctctagatc ggcgtgttat gttgtttccg 1140
tggttgcttg gttggatgaa tcgaatgatt cttagggcct aggaggctgg ttagatctgt 1200
tgcgttctgt ttcgtagatg gattttggtg taagatcagg tcggttccgc tgtttaactt 1260
gtgatgctag tgtgattttt gggaggattt gagttgttaa tctgggagtt gttgggaggt 1320
tctcgtaggc ggattgtaga tgaagtcgcc cgcacgattt gcgtggcttg ttgggtagct 1380
agggttagat ctgctcggat ttttcattgt tacttattga gagataatgt agctaacctt 1440
tacttgttca tctatgtatc tcgtattcgt attcatctgg ttcgatggtg ctagatagat 1500
gcgcctgatt tgtccgatcg aattgggtag catccgcggc ttgtttggta gtgttctgat 1560
tgatttgtcg ctctagatct gagtggaata atattacatc tcaacatgtt actagaaact 1620
tggtttatag ctccggattt acatgtttat tcttatgtaa ggttttaaat gaaagattta 1680
tgctactgct gctcgttgat cctttagcat ccacctgagg aacatgcatg catctgttac 1740
ttcttttgat atatgcttag atagttgtta gtatatactg ctgttgttcg atgatccttc 1800
aggatgaaca tgcatgatca tgttacttgt ttttatatgc ttctgctgtt cgttgattct 1860
ttagtactac ctacctgatc atcttgcatg tttcctgctt gttagagatt aattgattag 1920
gcttaccttg ttgcctggtg attcttcctt gcaggtg 1957
<210> 19
<211> 1957
<212> DNA
<213> Arundo donax
<400> 19
tgatgtatcc gtccacggtg gtgtgtccaa tcagtgaata atctagttag tgaagccaga 60
agtccatagt gccccttgct ctgtcaccat atatccagtt caaccgcacc aatttgccat 120
ctcgaactgg ttcatgtttt attcaggttg gtaaatgaat tttgccaatt caatgtagtt 180
agatatttcc atgtcatttt agtacattta ccaatttttt atattctggc tagaaaagga 240
gaatggtgac gtctttcgga agatcaagat caattatcaa gtatcagcaa cagcacctga 300
aggttggagt gcattagttg tcattgagaa taatgctagc tattcattgc actggcatta 360
gagacagaga gggcgagcca gtttgacatg gcaaattagc acagtcaaac tggatacgtg 420
gtgacggagg gaggggcact atgaattttt ggtgacggag ggaggggcac tatgaatttt 480
tggctttgct gacgggacac gccactatgg atgaaattgg acaaaatacg aatattcaag 540
gatgaaagtg gtcggtttga tagttcaggg atgaaatgtg tctttgggca aactttgagg 600
acgaagttgc ctattttgca ttaaacgaat atatttatat accccaaaaa aaagaataca 660
catctccact ccgagccggc atgtggggtc cccactagtc agccactgta tggcgccgac 720
tagctcaacg gccacgaacc agccaaccac cagcgcaacc taaacggcgt aaacgttgac 780
ggcatctctc tctcgccccg tctcgaagct tccgcaccgc tcgctggtcg ctgcccggcg 840
ccgctcgtgc tggactcttt ccgtggcggc ttccgcgaaa ttgcgtggtg gagaggagag 900
acggaaccgt cacggcactg gattccttcc ccacccggct tggccggccc ctcctcgcct 960
ccataaatag gcaccccgtc ctcgcctcct ctccccacct catctcctcc tttcccgtga 1020
accgtgaaca caacccgacc cagatcccct cttgcgagct tcgtcgatcc ctcctccgcg 1080
tcaaggtacg gagcttctcc tcccccttct tctctagatc ggcgtgttat gttgtttccg 1140
tggttgcttg gttggatgaa tcgaatgatt cttagggcct aggaggctgg ttagatctgt 1200
tgcgttctgt ttcgtagatg gattttggtg taagatcagg tcggttccgc tgtttaactt 1260
gtgatgctag tgtgattttt gggaggattt gagttgttaa tctgggagtt gttgggaggt 1320
tctcgtaggc ggattgtaga tgaagtcgcc cgcacgattt gcgtggcttg ttgggtagct 1380
agggttagat ctgctcggat ttttcattgt tacttattga gagataatgt agctaacctt 1440
tacttgttca tctatgtata tcgtattcgt attcatctgg ttcgatggtg ctagatagat 1500
gcgcctgatt tgtccgatcg aattgggtag catccgcggc ttgtttggta gtgttctgat 1560
tgatttgtcg ctctagatct gagtggaata atattacatc tcaacatgtt actagaaact 1620
tggtttatag ctccggattt acatgtttat tcttatgtaa ggttttaaat gaaagattta 1680
tgctactgct gctcgttgat cctttagcat ccacctgagg aacatgcatg catctgttac 1740
ttcttttgat atatgcttag atagttgtta gtatatactg ctgttgttcg atgatccttc 1800
aggatgaaca tgcatgatca tgttacttgt ttttatatgc ttctgctgtt cgttgattct 1860
ttagtactac ctacctgatc atcttgcatg tttcctgctt gttagagatt aattgattag 1920
gcttaccttg ttgcctggtg attcttcctt gcaggtg 1957
<210> 20
<211> 872
<212> DNA
<213> Arundo donax
<400> 20
gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt 60
gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt 120
tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat 180
gctagtgtga tttttgggag gatttgagtt gttaatctgg gagttgttgg gaggttctcg 240
taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt 300
tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt 360
gttcatctat gtatatcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc 420
tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt 480
tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt 540
tatagctccg gatttacatg tttattctta tgtaaggttt taaatgaaag atttatgcta 600
ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt 660
ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat 720
gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt 780
actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta 840
ccttgttgcc tggtgattct tccttgcagg tg 872
<210> 21
<211> 1712
<212> DNA
<213> Arundo donax
<400> 21
gtgacgtctt tcggaagatc aagatcaatt atcaagtatc agcaacagca cctgaaggtt 60
ggagtgcatt agttgtcatt gagaataatg ctagctattc attgcactgg cattagagac 120
agagagggcg agccagtttg acatggcaaa ttagcacagt caaactggat acgtggtgac 180
ggagggaggg gcactatgaa tttttggtga cggagggagg ggcactatga atttttggct 240
ttgctgacgg gacacgccac tatggatgaa attggacaaa atacgaatat tcaaggatga 300
aagtggtcgg tttgatagtt cagggatgaa atgtgtcttt gggcaaactt tgaggacgaa 360
gttgcctatt ttgcattaaa cgaatatatt tatatacccc aaaaaaaaga atacacatct 420
ccactccgag ccggcatgtg gggtccccac tagtcagcca ctgtatggcg ccgactagct 480
caacggccac gaaccagcca accaccagcg caacctaaac ggcgtaaacg ttgacggcat 540
ctctctctcg ccccgtctcg aagcttccgc accgctcgct ggtcgctgcc cggcgccgct 600
cgtgctggac tctttccgtg gcggcttccg cgaaattgcg tggtggagag gagagacgga 660
accgtcacgg cactggattc cttccccacc cggcttggcc ggcccctcct cgcctccata 720
aataggcacc ccgtcctcgc ctcctctccc cacctcatct cctcctttcc cgtgaaccgt 780
gaacacaacc cgacccagat cccctcttgc gagcttcgtc gatccctcct ccgcgtcaag 840
gtacggagct tctcctcccc cttcttctct agatcggcgt gttatgttgt ttccgtggtt 900
gcttggttgg atgaatcgaa tgattcttag ggcctaggag gctggttaga tctgttgcgt 960
tctgtttcgt agatggattt tggtgtaaga tcaggtcggt tccgctgttt aacttgtgat 1020
gctagtgtga tttttgggag gatttgagtt gttaatctgg gagttgttgg gaggttctcg 1080
taggcggatt gtagatgaag tcgcccgcac gatttgcgtg gcttgttggg tagctagggt 1140
tagatctgct cggatttttc attgttactt attgagagat aatgtagcta acctttactt 1200
gttcatctat gtatctcgta ttcgtattca tctggttcga tggtgctaga tagatgcgcc 1260
tgatttgtcc gatcgaattg ggtagcatcc gcggcttgtt tggtagtgtt ctgattgatt 1320
tgtcgctcta gatctgagtg gaataatatt acatctcaac atgttactag aaacttggtt 1380
tatagctccg gatttacatg tttattctta tgtaaggttt taaatgaaag atttatgcta 1440
ctgctgctcg ttgatccttt agcatccacc tgaggaacat gcatgcatct gttacttctt 1500
ttgatatatg cttagatagt tgttagtata tactgctgtt gttcgatgat ccttcaggat 1560
gaacatgcat gatcatgtta cttgttttta tatgcttctg ctgttcgttg attctttagt 1620
actacctacc tgatcatctt gcatgtttcc tgcttgttag agattaattg attaggctta 1680
ccttgttgcc tggtgattct tccttgcagg tg 1712
<210> 22
<211> 755
<212> DNA
<213> Arundo donax
<400> 22
gtgacgtctt tcggaagatc aagatcaatt atcaagtatc agcaacagca cctgaaggtt 60
ggagtgcatt agttgtcatt gagaataatg ctagctattc attgcactgg cattagagac 120
agagagggcg agccagtttg acatggcaaa ttagcacagt caaactggat acgtggtgac 180
ggagggaggg gcactatgaa tttttggtga cggagggagg ggcactatga atttttggct 240
ttgctgacgg gacacgccac tatggatgaa attggacaaa atacgaatat tcaaggatga 300
aagtggtcgg tttgatagtt cagggatgaa atgtgtcttt gggcaaactt tgaggacgaa 360
gttgcctatt ttgcattaaa cgaatatatt tatatacccc aaaaaaaaga atacacatct 420
ccactccgag ccggcatgtg gggtccccac tagtcagcca ctgtatggcg ccgactagct 480
caacggccac gaaccagcca accaccagcg caacctaaac ggcgtaaacg ttgacggcat 540
ctctctctcg ccccgtctcg aagcttccgc accgctcgct ggtcgctgcc cggcgccgct 600
cgtgctggac tctttccgtg gcggcttccg cgaaattgcg tggtggagag gagagacgga 660
accgtcacgg cactggattc cttccccacc cggcttggcc ggcccctcct cgcctccata 720
aataggcacc ccgtcctcgc ctcctctccc cacct 755
<210> 23
<211> 3276
<212> DNA
<213> Arundo donax
<400> 23
ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttag cctttgaagc 60
ttgaaccggg cactaatgca agtatataat aactgagaac tacaagaaag catattcctt 120
gaggtactta tgcaacctta caattatcaa attaattaac aactagcagt tagaatttta 180
tatcacaaga atatcatgaa ccgtggatac tacttcttaa agggctattc tttttctgaa 240
tgtcgcagtt ggttatttta accatattac aaactagggg tttaaatccc aaaaagttca 300
cggaaaggga ttaagcaagt agttagcaag actcacactt atgaccgtta gccaaattac 360
acattggtca ttccaggagg agtaatcccc catagctagt tgttttgagt ttgactaccc 420
aaacttgcat aatcgttttc ctagaggggg ggggggggtt caccattcca tcaagatgag 480
gcaaagctaa atgaaacaca cgagaggcaa aacggactga cgtgatagag tttttaataa 540
atatcaaata tgtagagtca accaaagaaa aaagatatcc caatggctaa actttggatc 600
tatgtcgtaa ttcgtgtttt aggacataca aggcgaattc cttctacggc aaactctaga 660
atagctgggc gacaatggcc tcacgatagc ctcaaagagt tggtagcaac tttgagatct 720
tttgatccga aactcaatta tgtagtacaa tgatatttag atagattgat tgaaagttgg 780
gggtgggggc gaaagcgaag gggatctcaa tattaataca tctatagtga atggatatag 840
aaaacacagg atttccaatt caagtagaaa taggaggaac ggaacagatc tagcaatagt 900
agccaccaaa gacgaggagg attctagatt gcaaatccaa ggtgaaagga agaaatgttg 960
aactatccag aataaggcgg attggccaag gaggcggaag tctctagaaa gaagtcattt 1020
ggctctgagg gctcacttga tgcgagaagg aagactgact gaggaatgga ttttggtgga 1080
ccgaggaaat tggtgctggg ttgcagaggc atgtatgtgg gaaaagaggc agtggcaacg 1140
atcgagagag gagaagggaa tgaggtaagt atttgaagtg aagaggagcc catataggtg 1200
aaaaataaaa ataatccatc gtggattcaa ataatcaaag ggctatgacc tttcatcaat 1260
tttagaaaag tgaaaacaac cggtttaaca cctatatgca ccattttcct acatagattt 1320
ttaacttctt acttaaccat gttgactaag agcaagtgga gagcactctc atttcataga 1380
acaagtgatg aatgccaacc tgcattatta tcttaattag actttgatca tcaagtggaa 1440
tcccatttat cttaataatc ttggcaacat tgttataatg ctacttcata tgctaattct 1500
tcaaagctaa catcgttaaa cgaatacata tctcctgtat tctaagaccc tatttagaat 1560
acagaaattt tacagaaatc agttcaattc tcgtagaatt gggaaagaaa tcctccgttc 1620
caaacgtgac ctaagccggc atggcacgac cccactcgtc aggcactgta tgtaaacgtc 1680
agcaactccg tggcaagtaa cgtcgagagg aggagcgggc ctaacggcgc cgactagctc 1740
aacggccacc aaccagccaa ccaccagcgc aaccgaaacg gcgcaaacgt tgacgtcatc 1800
tctctctctc tcgcgccccg cgtcccgaag cttccgcacc actcgctggt cgctgctagc 1860
tgggccccac cggccggccc cgttcgtgct ggactcttct tcctcgaaat tgcgtggtgg 1920
agagggagag ggggcacctc gagacggaac cgtcacggca cgggattcct tccccacccg 1980
gcccctcctc gtctccataa ataggcgccc cctcctcgcg tcctctcccc cgtctcatct 2040
cctcctgttc cgtgaaccgt gaacgcaacc cgacccccag atctctctcg cgagcatcgt 2100
cgatccctcc tccgcgtcaa ggtacggatc ttctccttcc tcccccttcc cctctgggtc 2160
ggcgtgtcgt gttgtttctc tagttgcttg gctggatgga tcgagtggtt cttagggctt 2220
agatggctgg ttagatctgt tgcgttctgt ttcgtagatg gatttttggt gtagatctgg 2280
taggttatgc tggttaactg gtgatgctcc tgcgattttt gggggatctg agttgttaat 2340
ctggtagttg tatggggttc tcgtagccgg attgtagatg aaatcgtccg cgcggtttgc 2400
gtggctcgtt ggttagctag ggttagatct gctcggattt ttcattgttc ctgattcaga 2460
gatgtagtta acctttactt gttcatcttt gtatctcgta ttcgtacctg catgtatgat 2520
ctgtttcgat ggtgctagat aggtgcgcct gatttgtccg atcgaatctg gtagcatgcg 2580
ctgtttgttt ggtagtgttc tgattgattt gtcgctctag atctgagtag aataggatta 2640
tttctcaaca tgatattaga agcttggttt atagctccgg attagcatgt atgttacatg 2700
tttattctta tgtaaggttt taaacggaag atatatgcta ctgctgctca ttgattcttt 2760
atcatccacc tgagtccatg catgcttctg ttacttcttt tgatatgtgc ttagatagct 2820
gttgatatgt actgctgctg ttagatgatc cttcaggatg aacatgcatg attctgttac 2880
ttgttttggt atgcttagat aaatcaagat acgcttctgc tgttcgttga ttctttagta 2940
ctacctacct gatcagctta gatagatcaa gatatgcttc tgctgttcgt tgattcttta 3000
gtaataccta cctgatcagc ttagatagat caagatacgc ttctgctgtt cgttgattct 3060
ctagtactac ctacctgata aacatgcatg ttttctgctt gttaaaggtt gattgcttag 3120
gctcatcttt ttcttttcgt tgattctcta gtactaccta cctgataaac atgcatgttt 3180
tctgcttgtt aaagattgat tgcttagtct catctttttc tttctctttt gtctaccgcc 3240
aggcctaacc ttgttgctgg tgactctttc ttgcag 3276
<210> 24
<211> 2033
<212> DNA
<213> Arundo donax
<400> 24
ggcctcttta cgtttggcac aatttgatcg aatccaacac ggcaagttag cctttgaagc 60
ttgaaccggg cactaatgca agtatataat aactgagaac tacaagaaag catattcctt 120
gaggtactta tgcaacctta caattatcaa attaattaac aactagcagt tagaatttta 180
tatcacaaga atatcatgaa ccgtggatac tacttcttaa agggctattc tttttctgaa 240
tgtcgcagtt ggttatttta accatattac aaactagggg tttaaatccc aaaaagttca 300
cggaaaggga ttaagcaagt agttagcaag actcacactt atgaccgtta gccaaattac 360
acattggtca ttccaggagg agtaatcccc catagctagt tgttttgagt ttgactaccc 420
aaacttgcat aatcgttttc ctagaggggg ggggggggtt caccattcca tcaagatgag 480
gcaaagctaa atgaaacaca cgagaggcaa aacggactga cgtgatagag tttttaataa 540
atatcaaata tgtagagtca accaaagaaa aaagatatcc caatggctaa actttggatc 600
tatgtcgtaa ttcgtgtttt aggacataca aggcgaattc cttctacggc aaactctaga 660
atagctgggc gacaatggcc tcacgatagc ctcaaagagt tggtagcaac tttgagatct 720
tttgatccga aactcaatta tgtagtacaa tgatatttag atagattgat tgaaagttgg 780
gggtgggggc gaaagcgaag gggatctcaa tattaataca tctatagtga atggatatag 840
aaaacacagg atttccaatt caagtagaaa taggaggaac ggaacagatc tagcaatagt 900
agccaccaaa gacgaggagg attctagatt gcaaatccaa ggtgaaagga agaaatgttg 960
aactatccag aataaggcgg attggccaag gaggcggaag tctctagaaa gaagtcattt 1020
ggctctgagg gctcacttga tgcgagaagg aagactgact gaggaatgga ttttggtgga 1080
ccgaggaaat tggtgctggg ttgcagaggc atgtatgtgg gaaaagaggc agtggcaacg 1140
atcgagagag gagaagggaa tgaggtaagt atttgaagtg aagaggagcc catataggtg 1200
aaaaataaaa ataatccatc gtggattcaa ataatcaaag ggctatgacc tttcatcaat 1260
tttagaaaag tgaaaacaac cggtttaaca cctatatgca ccattttcct acatagattt 1320
ttaacttctt acttaaccat gttgactaag agcaagtgga gagcactctc atttcataga 1380
acaagtgatg aatgccaacc tgcattatta tcttaattag actttgatca tcaagtggaa 1440
tcccatttat cttaataatc ttggcaacat tgttataatg ctacttcata tgctaattct 1500
tcaaagctaa catcgttaaa cgaatacata tctcctgtat tctaagaccc tatttagaat 1560
acagaaattt tacagaaatc agttcaattc tcgtagaatt gggaaagaaa tcctccgttc 1620
caaacgtgac ctaagccggc atggcacgac cccactcgtc aggcactgta tgtaaacgtc 1680
agcaactccg tggcaagtaa cgtcgagagg aggagcgggc ctaacggcgc cgactagctc 1740
aacggccacc aaccagccaa ccaccagcgc aaccgaaacg gcgcaaacgt tgacgtcatc 1800
tctctctctc tcgcgccccg cgtcccgaag cttccgcacc actcgctggt cgctgctagc 1860
tgggccccac cggccggccc cgttcgtgct ggactcttct tcctcgaaat tgcgtggtgg 1920
agagggagag ggggcacctc gagacggaac cgtcacggca cgggattcct tccccacccg 1980
gcccctcctc gtctccataa ataggcgccc cctcctcgcg tcctctcccc cgt 2033
<210> 25
<211> 88
<212> DNA
<213> Arundo donax
<400> 25
ctcatctcct cctgttccgt gaaccgtgaa cgcaacccga cccccagatc tctctcgcga 60
gcatcgtcga tccctcctcc gcgtcaag 88
<210> 26
<211> 1155
<212> DNA
<213> Arundo donax
<400> 26
gtacggatct tctccttcct cccccttccc ctctgggtcg gcgtgtcgtg ttgtttctct 60
agttgcttgg ctggatggat cgagtggttc ttagggctta gatggctggt tagatctgtt 120
gcgttctgtt tcgtagatgg atttttggtg tagatctggt aggttatgct ggttaactgg 180
tgatgctcct gcgatttttg ggggatctga gttgttaatc tggtagttgt atggggttct 240
cgtagccgga ttgtagatga aatcgtccgc gcggtttgcg tggctcgttg gttagctagg 300
gttagatctg ctcggatttt tcattgttcc tgattcagag atgtagttaa cctttacttg 360
ttcatctttg tatctcgtat tcgtacctgc atgtatgatc tgtttcgatg gtgctagata 420
ggtgcgcctg atttgtccga tcgaatctgg tagcatgcgc tgtttgtttg gtagtgttct 480
gattgatttg tcgctctaga tctgagtaga ataggattat ttctcaacat gatattagaa 540
gcttggttta tagctccgga ttagcatgta tgttacatgt ttattcttat gtaaggtttt 600
aaacggaaga tatatgctac tgctgctcat tgattcttta tcatccacct gagtccatgc 660
atgcttctgt tacttctttt gatatgtgct tagatagctg ttgatatgta ctgctgctgt 720
tagatgatcc ttcaggatga acatgcatga ttctgttact tgttttggta tgcttagata 780
aatcaagata cgcttctgct gttcgttgat tctttagtac tacctacctg atcagcttag 840
atagatcaag atatgcttct gctgttcgtt gattctttag taatacctac ctgatcagct 900
tagatagatc aagatacgct tctgctgttc gttgattctc tagtactacc tacctgataa 960
acatgcatgt tttctgcttg ttaaaggttg attgcttagg ctcatctttt tcttttcgtt 1020
gattctctag tactacctac ctgataaaca tgcatgtttt ctgcttgtta aagattgatt 1080
gcttagtctc atctttttct ttctcttttg tctaccgcca ggcctaacct tgttgctggt 1140
gactctttct tgcag 1155
<210> 27
<211> 3250
<212> DNA
<213> Arundo donax
<400> 27
gaatccaaca cggcaagtta gcctttgaag cttgaaccgg gcactaatgc aagtatataa 60
taactgagaa ctacaagaaa gcatattcct tgaggtactt atgcaacctt acaattatca 120
aattaattaa caactagcag ttagaatttt atatcacaag aatatcatga accgtggata 180
ctacttctta aagggctatt ctttttctga atgtcgcagt tggttatttt aaccatatta 240
caaactaggg gtttaaatcc caaaaagttc acggaaaggg attaagcaag tagttagcaa 300
gactcacact tatgaccgtt agccaaatta cacattggtc attccaggag gagtaatccc 360
ccatagctag ttgttttgag tttgactacc caaacttgca taatcgtttt cctagagggg 420
gggggggggt tcaccattcc atcaagatga ggcaaagcta aatgaaacac acgagaggca 480
aaacggactg acgtgataga gtttttaata aatatcaaat atgtagagtc aaccaaagaa 540
aaaagatatc ccaatggcta aactttggat ctatgtcgta attcgtgttt taggacatac 600
aaggcgaatt ccttctacgg caaactctag aatagctggg cgacaatggc ctcacgatag 660
cctcaaagag ttggtagcaa ctttgagatc ttttgatccg aaactcaatt atgtagtaca 720
atgatattta gatagattga ttgaaagttg ggggtggggg cgaaagcgaa ggggatctca 780
atattaatac atctatagtg aatggatata gaaaacacag gatttccaat tcaagtagaa 840
ataggaggaa cggaacagat ctagcaatag tagccaccaa agacgaggag gattctagat 900
tgcaaatcca aggtgaaagg aagaaatgtt gaactatcca gaataaggcg gattggccaa 960
ggaggcggaa gtctctagaa agaagtcatt tggctctgag ggctcacttg atgcgagaag 1020
gaagactgac tgaggaatgg attttggtgg accgaggaaa ttggtgctgg gttgcagagg 1080
catgtatgtg ggaaaagagg cagtggcaac gatcgagaga ggagaaggga atgaggtaag 1140
tatttgaagt gaagaggagc ccatataggt gaaaaataaa aataatccat cgtggattca 1200
aataatcaaa gggctatgac ctttcatcaa ttttagaaaa gtgaaaacaa ccggtttaac 1260
acctatatgc accattttcc tacatagatt tttaacttct tacttaacca tgttgactaa 1320
gagcaagtgg agagcactct catttcatag aacaagtgat gaatgccaac ctgcattatt 1380
atcttaatta gactttgatc atcaagtgga atcccattta tcttaataat cttggcaaca 1440
ttgttataat gctacttcat atgctaattc ttcaaagcta acatcgttaa acgaatacat 1500
atctcctgta ttctaagacc ctatttagaa tacagaaatt ttacagaaat cagttcaatt 1560
ctcgtagaat tgggaaagaa atcctccgtt ccaaacgtga cctaagccgg catggcacga 1620
ccccactcgt caggcactgt atgtaaacgt cagcaactcc gtggcaagta acgtcgagag 1680
gaggagcggg cctaacggcg ccgactagct caacggccac caaccagcca accaccagcg 1740
caaccgaaac ggcgcaaacg ttgacgtcat ctctctctct ctcgcgcccc gcgtcccgaa 1800
gcttccgcac cactcgctgg tcgctgctag ctgggcccca ccggccggcc ccgttcgtgc 1860
tggactcttc ttcctcgaaa ttgcgtggtg gagagggaga gggggcacct cgagacggaa 1920
ccgtcacggc acgggattcc ttccccaccc ggcccctcct cgtctccata aataggcgcc 1980
ccctcctcgc gtcctctccc ccgtctcatc tcctcctgtt ccgtgaaccg tgaacgcaac 2040
ccgaccccca gatctctctc gcgagcatcg tcgatccctc ctccgcgtca aggtacggat 2100
cttctccttc ctcccccttc ccctctgggt cggcgtgtcg tgttgtttct ctagttgctt 2160
ggctggatgg atcgagtggt tcttagggct tagatggctg gttagatctg ttgcgttctg 2220
tttcgtagat ggatttttgg tgtagatctg gtaggttatg ctggttaact ggtgatgctc 2280
ctgcgatttt tgggggatct gagttgttaa tctggtagtt gtatggggtt ctcgtagccg 2340
gattgtagat gaaatcgtcc gcgcggtttg cgtggctcgt tggttagcta gggttagatc 2400
tgctcggatt tttcattgtt cctgattcag agatgtagtt aacctttact tgttcatctt 2460
tgtatctcgt attcgtacct gcatgtatga tctgtttcga tggtgctaga taggtgcgcc 2520
tgatttgtcc gatcgaatct ggtagcatgc gctgtttgtt tggtagtgtt ctgattgatt 2580
tgtcgctcta gatctgagta gaataggatt atttctcaac atgatattag aagcttggtt 2640
tatagctccg gattagcatg tatgttacat gtttattctt atgtaaggtt ttaaacggaa 2700
gatatatgct actgctgctc attgattctt tatcatccac ctgagtccat gcatgcttct 2760
gttacttctt ttgatatgtg cttagatagc tgttgatatg tactgctgct gttagatgat 2820
ccttcaggat gaacatgcat gattctgtta cttgttttgg tatgcttaga taaatcaaga 2880
tacgcttctg ctgttcgttg attctttagt actacctacc tgatcagctt agatagatca 2940
agatatgctt ctgctgttcg ttgattcttt agtaatacct acctgatcag cttagataga 3000
tcaagatacg cttctgctgt tcgttgattc tctagtacta cctacctgat aaacatgcat 3060
gttttctgct tgttaaaggt tgattgctta ggctcatctt tttcttttcg ttgattctct 3120
agtactacct acctgataaa catgcatgtt ttctgcttgt taaagattga ttgcttagtc 3180
tcatcttttt ctttctcttt tgtctaccgc caggcctaac cttgttgctg gtgactcttt 3240
cttgcaggtg 3250
<210> 28
<211> 2004
<212> DNA
<213> Arundo donax
<400> 28
gaatccaaca cggcaagtta gcctttgaag cttgaaccgg gcactaatgc aagtatataa 60
taactgagaa ctacaagaaa gcatattcct tgaggtactt atgcaacctt acaattatca 120
aattaattaa caactagcag ttagaatttt atatcacaag aatatcatga accgtggata 180
ctacttctta aagggctatt ctttttctga atgtcgcagt tggttatttt aaccatatta 240
caaactaggg gtttaaatcc caaaaagttc acggaaaggg attaagcaag tagttagcaa 300
gactcacact tatgaccgtt agccaaatta cacattggtc attccaggag gagtaatccc 360
ccatagctag ttgttttgag tttgactacc caaacttgca taatcgtttt cctagagggg 420
gggggggggt tcaccattcc atcaagatga ggcaaagcta aatgaaacac acgagaggca 480
aaacggactg acgtgataga gtttttaata aatatcaaat atgtagagtc aaccaaagaa 540
aaaagatatc ccaatggcta aactttggat ctatgtcgta attcgtgttt taggacatac 600
aaggcgaatt ccttctacgg caaactctag aatagctggg cgacaatggc ctcacgatag 660
cctcaaagag ttggtagcaa ctttgagatc ttttgatccg aaactcaatt atgtagtaca 720
atgatattta gatagattga ttgaaagttg ggggtggggg cgaaagcgaa ggggatctca 780
atattaatac atctatagtg aatggatata gaaaacacag gatttccaat tcaagtagaa 840
ataggaggaa cggaacagat ctagcaatag tagccaccaa agacgaggag gattctagat 900
tgcaaatcca aggtgaaagg aagaaatgtt gaactatcca gaataaggcg gattggccaa 960
ggaggcggaa gtctctagaa agaagtcatt tggctctgag ggctcacttg atgcgagaag 1020
gaagactgac tgaggaatgg attttggtgg accgaggaaa ttggtgctgg gttgcagagg 1080
catgtatgtg ggaaaagagg cagtggcaac gatcgagaga ggagaaggga atgaggtaag 1140
tatttgaagt gaagaggagc ccatataggt gaaaaataaa aataatccat cgtggattca 1200
aataatcaaa gggctatgac ctttcatcaa ttttagaaaa gtgaaaacaa ccggtttaac 1260
acctatatgc accattttcc tacatagatt tttaacttct tacttaacca tgttgactaa 1320
gagcaagtgg agagcactct catttcatag aacaagtgat gaatgccaac ctgcattatt 1380
atcttaatta gactttgatc atcaagtgga atcccattta tcttaataat cttggcaaca 1440
ttgttataat gctacttcat atgctaattc ttcaaagcta acatcgttaa acgaatacat 1500
atctcctgta ttctaagacc ctatttagaa tacagaaatt ttacagaaat cagttcaatt 1560
ctcgtagaat tgggaaagaa atcctccgtt ccaaacgtga cctaagccgg catggcacga 1620
ccccactcgt caggcactgt atgtaaacgt cagcaactcc gtggcaagta acgtcgagag 1680
gaggagcggg cctaacggcg ccgactagct caacggccac caaccagcca accaccagcg 1740
caaccgaaac ggcgcaaacg ttgacgtcat ctctctctct ctcgcgcccc gcgtcccgaa 1800
gcttccgcac cactcgctgg tcgctgctag ctgggcccca ccggccggcc ccgttcgtgc 1860
tggactcttc ttcctcgaaa ttgcgtggtg gagagggaga gggggcacct cgagacggaa 1920
ccgtcacggc acgggattcc ttccccaccc ggcccctcct cgtctccata aataggcgcc 1980
ccctcctcgc gtcctctccc ccgt 2004
<210> 29
<211> 1158
<212> DNA
<213> Arundo donax
<400> 29
gtacggatct tctccttcct cccccttccc ctctgggtcg gcgtgtcgtg ttgtttctct 60
agttgcttgg ctggatggat cgagtggttc ttagggctta gatggctggt tagatctgtt 120
gcgttctgtt tcgtagatgg atttttggtg tagatctggt aggttatgct ggttaactgg 180
tgatgctcct gcgatttttg ggggatctga gttgttaatc tggtagttgt atggggttct 240
cgtagccgga ttgtagatga aatcgtccgc gcggtttgcg tggctcgttg gttagctagg 300
gttagatctg ctcggatttt tcattgttcc tgattcagag atgtagttaa cctttacttg 360
ttcatctttg tatctcgtat tcgtacctgc atgtatgatc tgtttcgatg gtgctagata 420
ggtgcgcctg atttgtccga tcgaatctgg tagcatgcgc tgtttgtttg gtagtgttct 480
gattgatttg tcgctctaga tctgagtaga ataggattat ttctcaacat gatattagaa 540
gcttggttta tagctccgga ttagcatgta tgttacatgt ttattcttat gtaaggtttt 600
aaacggaaga tatatgctac tgctgctcat tgattcttta tcatccacct gagtccatgc 660
atgcttctgt tacttctttt gatatgtgct tagatagctg ttgatatgta ctgctgctgt 720
tagatgatcc ttcaggatga acatgcatga ttctgttact tgttttggta tgcttagata 780
aatcaagata cgcttctgct gttcgttgat tctttagtac tacctacctg atcagcttag 840
atagatcaag atatgcttct gctgttcgtt gattctttag taatacctac ctgatcagct 900
tagatagatc aagatacgct tctgctgttc gttgattctc tagtactacc tacctgataa 960
acatgcatgt tttctgcttg ttaaaggttg attgcttagg ctcatctttt tcttttcgtt 1020
gattctctag tactacctac ctgataaaca tgcatgtttt ctgcttgtta aagattgatt 1080
gcttagtctc atctttttct ttctcttttg tctaccgcca ggcctaacct tgttgctggt 1140
gactctttct tgcaggtg 1158
<210> 30
<211> 2247
<212> DNA
<213> Arundo donax
<400> 30
tcacttgatg cgagaaggaa gactgactga ggaatggatt ttggtggacc gaggaaattg 60
gtgctgggtt gcagaggcat gtatgtggga aaagaggcag tggcaacgat cgagagagga 120
gaagggaatg aggtaagtat ttgaagtgaa gaggagccca tataggtgaa aaataaaaat 180
aatccatcgt ggattcaaat aatcaaaggg ctatgacctt tcatcaattt tagaaaagtg 240
aaaacaaccg gtttaacacc tatatgcacc attttcctac atagattttt aacttcttac 300
ttaaccatgt tgactaagag caagtggaga gcactctcat ttcatagaac aagtgatgaa 360
tgccaacctg cattattatc ttaattagac tttgatcatc aagtggaatc ccatttatct 420
taataatctt ggcaacattg ttataatgct acttcatatg ctaattcttc aaagctaaca 480
tcgttaaacg aatacatatc tcctgtattc taagacccta tttagaatac agaaatttta 540
cagaaatcag ttcaattctc gtagaattgg gaaagaaatc ctccgttcca aacgtgacct 600
aagccggcat ggcacgaccc cactcgtcag gcactgtatg taaacgtcag caactccgtg 660
gcaagtaacg tcgagaggag gagcgggcct aacggcgccg actagctcaa cggccaccaa 720
ccagccaacc accagcgcaa ccgaaacggc gcaaacgttg acgtcatctc tctctctctc 780
gcgccccgcg tcccgaagct tccgcaccac tcgctggtcg ctgctagctg ggccccaccg 840
gccggccccg ttcgtgctgg actcttcttc ctcgaaattg cgtggtggag agggagaggg 900
ggcacctcga gacggaaccg tcacggcacg ggattccttc cccacccggc ccctcctcgt 960
ctccataaat aggcgccccc tcctcgcgtc ctctcccccg tctcatctcc tcctgttccg 1020
tgaaccgtga acgcaacccg acccccagat ctctctcgcg agcatcgtcg atccctcctc 1080
cgcgtcaagg tacggatctt ctccttcctc ccccttcccc tctgggtcgg cgtgtcgtgt 1140
tgtttctcta gttgcttggc tggatggatc gagtggttct tagggcttag atggctggtt 1200
agatctgttg cgttctgttt cgtagatgga tttttggtgt agatctggta ggttatgctg 1260
gttaactggt gatgctcctg cgatttttgg gggatctgag ttgttaatct ggtagttgta 1320
tggggttctc gtagccggat tgtagatgaa atcgtccgcg cggtttgcgt ggctcgttgg 1380
ttagctaggg ttagatctgc tcggattttt cattgttcct gattcagaga tgtagttaac 1440
ctttacttgt tcatctttgt atctcgtatt cgtacctgca tgtatgatct gtttcgatgg 1500
tgctagatag gtgcgcctga tttgtccgat cgaatctggt agcatgcgct gtttgtttgg 1560
tagtgttctg attgatttgt cgctctagat ctgagtagaa taggattatt tctcaacatg 1620
atattagaag cttggtttat agctccggat tagcatgtat gttacatgtt tattcttatg 1680
taaggtttta aacggaagat atatgctact gctgctcatt gattctttat catccacctg 1740
agtccatgca tgcttctgtt acttcttttg atatgtgctt agatagctgt tgatatgtac 1800
tgctgctgtt agatgatcct tcaggatgaa catgcatgat tctgttactt gttttggtat 1860
gcttagataa atcaagatac gcttctgctg ttcgttgatt ctttagtact acctacctga 1920
tcagcttaga tagatcaaga tatgcttctg ctgttcgttg attctttagt aatacctacc 1980
tgatcagctt agatagatca agatacgctt ctgctgttcg ttgattctct agtactacct 2040
acctgataaa catgcatgtt ttctgcttgt taaaggttga ttgcttaggc tcatcttttt 2100
cttttcgttg attctctagt actacctacc tgataaacat gcatgttttc tgcttgttaa 2160
agattgattg cttagtctca tctttttctt tctcttttgt ctaccgccag gcctaacctt 2220
gttgctggtg actctttctt gcaggtg 2247
<210> 31
<211> 1001
<212> DNA
<213> Arundo donax
<400> 31
tcacttgatg cgagaaggaa gactgactga ggaatggatt ttggtggacc gaggaaattg 60
gtgctgggtt gcagaggcat gtatgtggga aaagaggcag tggcaacgat cgagagagga 120
gaagggaatg aggtaagtat ttgaagtgaa gaggagccca tataggtgaa aaataaaaat 180
aatccatcgt ggattcaaat aatcaaaggg ctatgacctt tcatcaattt tagaaaagtg 240
aaaacaaccg gtttaacacc tatatgcacc attttcctac atagattttt aacttcttac 300
ttaaccatgt tgactaagag caagtggaga gcactctcat ttcatagaac aagtgatgaa 360
tgccaacctg cattattatc ttaattagac tttgatcatc aagtggaatc ccatttatct 420
taataatctt ggcaacattg ttataatgct acttcatatg ctaattcttc aaagctaaca 480
tcgttaaacg aatacatatc tcctgtattc taagacccta tttagaatac agaaatttta 540
cagaaatcag ttcaattctc gtagaattgg gaaagaaatc ctccgttcca aacgtgacct 600
aagccggcat ggcacgaccc cactcgtcag gcactgtatg taaacgtcag caactccgtg 660
gcaagtaacg tcgagaggag gagcgggcct aacggcgccg actagctcaa cggccaccaa 720
ccagccaacc accagcgcaa ccgaaacggc gcaaacgttg acgtcatctc tctctctctc 780
gcgccccgcg tcccgaagct tccgcaccac tcgctggtcg ctgctagctg ggccccaccg 840
gccggccccg ttcgtgctgg actcttcttc ctcgaaattg cgtggtggag agggagaggg 900
ggcacctcga gacggaaccg tcacggcacg ggattccttc cccacccggc ccctcctcgt 960
ctccataaat aggcgccccc tcctcgcgtc ctctcccccg t 1001
<210> 32
<211> 1942
<212> DNA
<213> Arundo donax
<400> 32
catgttgact aagagcaagt ggagagcact ctcatttcat agaacaagtg atgaatgcca 60
acctgcatta ttatcttaat tagactttga tcatcaagtg gaatcccatt tatcttaata 120
atcttggcaa cattgttata atgctacttc atatgctaat tcttcaaagc taacatcgtt 180
aaacgaatac atatctcctg tattctaaga ccctatttag aatacagaaa ttttacagaa 240
atcagttcaa ttctcgtaga attgggaaag aaatcctccg ttccaaacgt gacctaagcc 300
ggcatggcac gaccccactc gtcaggcact gtatgtaaac gtcagcaact ccgtggcaag 360
taacgtcgag aggaggagcg ggcctaacgg cgccgactag ctcaacggcc accaaccagc 420
caaccaccag cgcaaccgaa acggcgcaaa cgttgacgtc atctctctct ctctcgcgcc 480
ccgcgtcccg aagcttccgc accactcgct ggtcgctgct agctgggccc caccggccgg 540
ccccgttcgt gctggactct tcttcctcga aattgcgtgg tggagaggga gagggggcac 600
ctcgagacgg aaccgtcacg gcacgggatt ccttccccac ccggcccctc ctcgtctcca 660
taaataggcg ccccctcctc gcgtcctctc ccccgtctca tctcctcctg ttccgtgaac 720
cgtgaacgca acccgacccc cagatctctc tcgcgagcat cgtcgatccc tcctccgcgt 780
caaggtacgg atcttctcct tcctccccct tcccctctgg gtcggcgtgt cgtgttgttt 840
ctctagttgc ttggctggat ggatcgagtg gttcttaggg cttagatggc tggttagatc 900
tgttgcgttc tgtttcgtag atggattttt ggtgtagatc tggtaggtta tgctggttaa 960
ctggtgatgc tcctgcgatt tttgggggat ctgagttgtt aatctggtag ttgtatgggg 1020
ttctcgtagc cggattgtag atgaaatcgt ccgcgcggtt tgcgtggctc gttggttagc 1080
tagggttaga tctgctcgga tttttcattg ttcctgattc agagatgtag ttaaccttta 1140
cttgttcatc tttgtatctc gtattcgtac ctgcatgtat gatctgtttc gatggtgcta 1200
gataggtgcg cctgatttgt ccgatcgaat ctggtagcat gcgctgtttg tttggtagtg 1260
ttctgattga tttgtcgctc tagatctgag tagaatagga ttatttctca acatgatatt 1320
agaagcttgg tttatagctc cggattagca tgtatgttac atgtttattc ttatgtaagg 1380
ttttaaacgg aagatatatg ctactgctgc tcattgattc tttatcatcc acctgagtcc 1440
atgcatgctt ctgttacttc ttttgatatg tgcttagata gctgttgata tgtactgctg 1500
ctgttagatg atccttcagg atgaacatgc atgattctgt tacttgtttt ggtatgctta 1560
gataaatcaa gatacgcttc tgctgttcgt tgattcttta gtactaccta cctgatcagc 1620
ttagatagat caagatatgc ttctgctgtt cgttgattct ttagtaatac ctacctgatc 1680
agcttagata gatcaagata cgcttctgct gttcgttgat tctctagtac tacctacctg 1740
ataaacatgc atgttttctg cttgttaaag gttgattgct taggctcatc tttttctttt 1800
cgttgattct ctagtactac ctacctgata aacatgcatg ttttctgctt gttaaagatt 1860
gattgcttag tctcatcttt ttctttctct tttgtctacc gccaggccta accttgttgc 1920
tggtgactct ttcttgcagg tg 1942
<210> 33
<211> 696
<212> DNA
<213> Arundo donax
<400> 33
catgttgact aagagcaagt ggagagcact ctcatttcat agaacaagtg atgaatgcca 60
acctgcatta ttatcttaat tagactttga tcatcaagtg gaatcccatt tatcttaata 120
atcttggcaa cattgttata atgctacttc atatgctaat tcttcaaagc taacatcgtt 180
aaacgaatac atatctcctg tattctaaga ccctatttag aatacagaaa ttttacagaa 240
atcagttcaa ttctcgtaga attgggaaag aaatcctccg ttccaaacgt gacctaagcc 300
ggcatggcac gaccccactc gtcaggcact gtatgtaaac gtcagcaact ccgtggcaag 360
taacgtcgag aggaggagcg ggcctaacgg cgccgactag ctcaacggcc accaaccagc 420
caaccaccag cgcaaccgaa acggcgcaaa cgttgacgtc atctctctct ctctcgcgcc 480
ccgcgtcccg aagcttccgc accactcgct ggtcgctgct agctgggccc caccggccgg 540
ccccgttcgt gctggactct tcttcctcga aattgcgtgg tggagaggga gagggggcac 600
ctcgagacgg aaccgtcacg gcacgggatt ccttccccac ccggcccctc ctcgtctcca 660
taaataggcg ccccctcctc gcgtcctctc ccccgt 696
<210> 34
<211> 3511
<212> DNA
<213> Bouteloua gracilis
<400> 34
gtggccagct tttgttctag ttcaacggtc ccggccttcc gtgcacctaa tactacactg 60
attaatctat tgcagctaac ctcaaaagaa atacacttgc agttgtctgt cccaatcaag 120
ccactagcag actctcatgt cattgatgga ggaaattaaa ttcagtcttt gacgtggatg 180
caacaactgc acagtatacc atgcatctta attagccgtt gtgtcaaagt ttgttttgct 240
gacgttttga gaaaaccaac tttgaccaac aggagatgag cgtcttgcgt ttggcacagt 300
gtaatggaat ccggcacggc aagttagact ctgtagtgtt agcggtctct ttacgtttgg 360
cacaatttaa ttgaatcccg gcatggcatg ttagaccgga gtgagccggc ccttttactg 420
gtatgacact ccctctgtct tgagtgtcgc tgtgccagct tgtacctctg tctatgttca 480
cagcccgtgc tgtgtaccta gaccctccgt ttgtccacat tcattttaat ctctattgta 540
tcttgtcaaa acctaaaagc ctaaaacgac tctgataaag ggacagaaag attatacaag 600
agcaagtgta taatgaaata atgtaagcga gctatatgaa ttgtcacgtg tcatatttat 660
gttgagacga agaagagaaa ataaacacca tgcaaattta tggcgagtga tagatggcca 720
gatgggcaca aggcctccta tttcttaaat cggattttgt aagaacgaaa aaagggactt 780
ataagagaat aggatagacc atatatcaat gatgtagtat gcatcaagat ctaactatta 840
tatgagtgaa ttgataaatt tattctaggt gacatggcct taacgatgaa cagtacgtgg 900
ttaaatcaat agaacaatag ccaactctag cggctctaaa aaaagatata tattcgtcga 960
ggcactatta tgcaaccaca tagtcaactt caacgccgct tgagtgcgtt ctcatgtttt 1020
ttttttcttg caaattacgc ttttctaaaa taaaataatt tggatcgtgc aattatttca 1080
ctttaggtgt gcgtgactac gtgagtaaca attttgaatc tcagaaagga aataaaagta 1140
taatactgct acctactttg aggattcagc ttgttactta aaaccgtctt taaggtcaaa 1200
tgctcaagat tcattcaaca attgaaacgt ctcacatgat taaaccatgt ataaggatgc 1260
taaggtcttg cttgacaatg tttttctagg aatttcatct aactttttga gtgaaactat 1320
caaataataa ttttaaaaca attttataag agaagctccg gagataaaag ggcatctaat 1380
ctatgttaga agagtgaagt ttactccctc tgtcccaaaa atagaattct aagtatgaaa 1440
tgattttttt gttatacgaa aggagtatat atcacaagat tgatgtcagt tatgcttagg 1500
gcacgtacac gacgctggtg ctttaggtag acgttaatcg ttgtttctgc attttatttt 1560
attttgttgc cacggtgtac atttgggtag acgtttgtca caggcattgc cactcaaaca 1620
agcagccggc gcttggagct tttatagttt gaaaagtgac ggttttaatg atgggtaagc 1680
tgattagtat atgtaagttt agctttttcc attgtaggtt aagccttaag gctcttacac 1740
aattgtttca ttattctcat tctttaagag cccatataag cgttcatgaa ttgtacatat 1800
ccttagatgt tttttttttt gggtaaagct cgagcttctc tatctaaaag tagagaaatc 1860
agaaaaagat tcatgttttg gtagttttga tttcttgcct ccataataat tttggtttac 1920
cattttttgt ttgattttag ttttagaagc gtttatagca ggatttaaaa tccaaaacta 1980
ccattatctt caagtgaccg tcagtgagcc gtttaacggc gtcgacaagt ccaacggaca 2040
ccaaccagtg aaccaccagc gtcgagccaa gcgatgcaaa cggaacggcc gagacgttga 2100
cacctttggc gcggcacggc atgtcggatc tccctctctg gccagagagt tccagctcca 2160
cctccacctc cacctccacc ggtggcggtt tccaagtccg ttccgttccg ttccgttccg 2220
ttccgttccg cctcctgcct gctcctctca gacggcacga aaccgtgacg gcaccggcag 2280
cacgggggga ttccttttcc actgctcctt cctcttccct tcctcgcccg ccgctataaa 2340
tagccagccc cgtccccaga ttctttccca acctcatctt tgttcggagc acgcacacaa 2400
cccgatcccc aattccctcg tctctcctcg cgagcctcgt cgaccccccc cttcaaggta 2460
cggcgatcat cctccctccc tccctctctc taccttctct tctctagact agatcggcga 2520
cccggtccat ggttagggcc tgctagttct gttcctgttt tttccatggc tgcgaggtaa 2580
aatagatctg atggcgttat gatggttaac tcgtcatact cttgcgatct atggtccctt 2640
taggacatcg atttaatttc ggatggttcg agatcggtga tccatggtta gtaccctagg 2700
cagtggggtt agatccgtgc tgttagggtt cgtagatgga ttctgattgc tcagtaactg 2760
ggaaacctgg gatggttcta gctgggaatc ctgggatggt tctagctggt tcgcagatga 2820
gatcgatttc atggtctgct atatcttgtt tcgttgccta ggttccgttt aatctgtccg 2880
tggtatgatg ttagcctttg ataaggttcg atcgtgctag ctacgtcctg cgcagcattt 2940
aattgtcagg tcataatttt tagcattcct gtttttgttt ggtttggttt tgtctggttg 3000
ggctgtagat agtttcaatc tacctgtcgg tttattttat taaatttgga ttggatctgt 3060
atgtgtcaca tatatcttca tgattaagat ggttggaatt atctcttcat cttttagata 3120
tatatggata ggtatatatg ttgctgtggg ttttactggt actttattag atatattcat 3180
gcttagatac atgaagcaac gtgctgttac agtttaataa ttcttgttta tctaataaac 3240
aaataaggat aggtatatgt tgctgatggt tttactgata ctttattaga tagtactttg 3300
acatgaagga acatcctgcg acagcttaat aattattctt catctaataa aaagcttgct 3360
ttttaattat tttaattatt ttgatatact tggatgatgt catgcagcag ctatgtgtga 3420
attttcggcc ctgtcttcat atgatgttta tttgcttggg actgtttctt tggctgataa 3480
cttaccctgt tgtttggtga tccttctgca g 3511
<210> 35
<211> 2371
<212> DNA
<213> Bouteloua gracilis
<400> 35
gtggccagct tttgttctag ttcaacggtc ccggccttcc gtgcacctaa tactacactg 60
attaatctat tgcagctaac ctcaaaagaa atacacttgc agttgtctgt cccaatcaag 120
ccactagcag actctcatgt cattgatgga ggaaattaaa ttcagtcttt gacgtggatg 180
caacaactgc acagtatacc atgcatctta attagccgtt gtgtcaaagt ttgttttgct 240
gacgttttga gaaaaccaac tttgaccaac aggagatgag cgtcttgcgt ttggcacagt 300
gtaatggaat ccggcacggc aagttagact ctgtagtgtt agcggtctct ttacgtttgg 360
cacaatttaa ttgaatcccg gcatggcatg ttagaccgga gtgagccggc ccttttactg 420
gtatgacact ccctctgtct tgagtgtcgc tgtgccagct tgtacctctg tctatgttca 480
cagcccgtgc tgtgtaccta gaccctccgt ttgtccacat tcattttaat ctctattgta 540
tcttgtcaaa acctaaaagc ctaaaacgac tctgataaag ggacagaaag attatacaag 600
agcaagtgta taatgaaata atgtaagcga gctatatgaa ttgtcacgtg tcatatttat 660
gttgagacga agaagagaaa ataaacacca tgcaaattta tggcgagtga tagatggcca 720
gatgggcaca aggcctccta tttcttaaat cggattttgt aagaacgaaa aaagggactt 780
ataagagaat aggatagacc atatatcaat gatgtagtat gcatcaagat ctaactatta 840
tatgagtgaa ttgataaatt tattctaggt gacatggcct taacgatgaa cagtacgtgg 900
ttaaatcaat agaacaatag ccaactctag cggctctaaa aaaagatata tattcgtcga 960
ggcactatta tgcaaccaca tagtcaactt caacgccgct tgagtgcgtt ctcatgtttt 1020
ttttttcttg caaattacgc ttttctaaaa taaaataatt tggatcgtgc aattatttca 1080
ctttaggtgt gcgtgactac gtgagtaaca attttgaatc tcagaaagga aataaaagta 1140
taatactgct acctactttg aggattcagc ttgttactta aaaccgtctt taaggtcaaa 1200
tgctcaagat tcattcaaca attgaaacgt ctcacatgat taaaccatgt ataaggatgc 1260
taaggtcttg cttgacaatg tttttctagg aatttcatct aactttttga gtgaaactat 1320
caaataataa ttttaaaaca attttataag agaagctccg gagataaaag ggcatctaat 1380
ctatgttaga agagtgaagt ttactccctc tgtcccaaaa atagaattct aagtatgaaa 1440
tgattttttt gttatacgaa aggagtatat atcacaagat tgatgtcagt tatgcttagg 1500
gcacgtacac gacgctggtg ctttaggtag acgttaatcg ttgtttctgc attttatttt 1560
attttgttgc cacggtgtac atttgggtag acgtttgtca caggcattgc cactcaaaca 1620
agcagccggc gcttggagct tttatagttt gaaaagtgac ggttttaatg atgggtaagc 1680
tgattagtat atgtaagttt agctttttcc attgtaggtt aagccttaag gctcttacac 1740
aattgtttca ttattctcat tctttaagag cccatataag cgttcatgaa ttgtacatat 1800
ccttagatgt tttttttttt gggtaaagct cgagcttctc tatctaaaag tagagaaatc 1860
agaaaaagat tcatgttttg gtagttttga tttcttgcct ccataataat tttggtttac 1920
cattttttgt ttgattttag ttttagaagc gtttatagca ggatttaaaa tccaaaacta 1980
ccattatctt caagtgaccg tcagtgagcc gtttaacggc gtcgacaagt ccaacggaca 2040
ccaaccagtg aaccaccagc gtcgagccaa gcgatgcaaa cggaacggcc gagacgttga 2100
cacctttggc gcggcacggc atgtcggatc tccctctctg gccagagagt tccagctcca 2160
cctccacctc cacctccacc ggtggcggtt tccaagtccg ttccgttccg ttccgttccg 2220
ttccgttccg cctcctgcct gctcctctca gacggcacga aaccgtgacg gcaccggcag 2280
cacgggggga ttccttttcc actgctcctt cctcttccct tcctcgcccg ccgctataaa 2340
tagccagccc cgtccccaga ttctttccca a 2371
<210> 36
<211> 86
<212> DNA
<213> Bouteloua gracilis
<400> 36
cctcatcttt gttcggagca cgcacacaac ccgatcccca attccctcgt ctctcctcgc 60
gagcctcgtc gacccccccc ttcaag 86
<210> 37
<211> 1054
<212> DNA
<213> Bouteloua gracilis
<400> 37
gtacggcgat catcctccct ccctccctct ctctaccttc tcttctctag actagatcgg 60
cgacccggtc catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg 120
taaaatagat ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc 180
ctttaggaca tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct 240
aggcagtggg gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa 300
ctgggaaacc tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga 360
tgagatcgat ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt 420
ccgtggtatg atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca 480
tttaattgtc aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg 540
ttgggctgta gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc 600
tgtatgtgtc acatatatct tcatgattaa gatggttgga attatctctt catcttttag 660
atatatatgg ataggtatat atgttgctgt gggttttact ggtactttat tagatatatt 720
catgcttaga tacatgaagc aacgtgctgt tacagtttaa taattcttgt ttatctaata 780
aacaaataag gataggtata tgttgctgat ggttttactg atactttatt agatagtact 840
ttgacatgaa ggaacatcct gcgacagctt aataattatt cttcatctaa taaaaagctt 900
gctttttaat tattttaatt attttgatat acttggatga tgtcatgcag cagctatgtg 960
tgaattttcg gccctgtctt catatgatgt ttatttgctt gggactgttt ctttggctga 1020
taacttaccc tgttgtttgg tgatccttct gcag 1054
<210> 38
<211> 3142
<212> DNA
<213> Bouteloua gracilis
<400> 38
gaatcccggc atggcatgtt agaccggagt gagccggccc ttttactggt atgacactcc 60
ctctgtcttg agtgtcgctg tgccagcttg tacctctgtc tatgttcaca gcccgtgctg 120
tgtacctaga ccctccgttt gtccacattc attttaatct ctattgtatc ttgtcaaaac 180
ctaaaagcct aaaacgactc tgataaaggg acagaaagat tatacaagag caagtgtata 240
atgaaataat gtaagcgagc tatatgaatt gtcacgtgtc atatttatgt tgagacgaag 300
aagagaaaat aaacaccatg caaatttatg gcgagtgata gatggccaga tgggcacaag 360
gcctcctatt tcttaaatcg gattttgtaa gaacgaaaaa agggacttat aagagaatag 420
gatagaccat atatcaatga tgtagtatgc atcaagatct aactattata tgagtgaatt 480
gataaattta ttctaggtga catggcctta acgatgaaca gtacgtggtt aaatcaatag 540
aacaatagcc aactctagcg gctctaaaaa aagatatata ttcgtcgagg cactattatg 600
caaccacata gtcaacttca acgccgcttg agtgcgttct catgtttttt ttttcttgca 660
aattacgctt ttctaaaata aaataatttg gatcgtgcaa ttatttcact ttaggtgtgc 720
gtgactacgt gagtaacaat tttgaatctc agaaaggaaa taaaagtata atactgctac 780
ctactttgag gattcagctt gttacttaaa accgtcttta aggtcaaatg ctcaagattc 840
attcaacaat tgaaacgtct cacatgatta aaccatgtat aaggatgcta aggtcttgct 900
tgacaatgtt tttctaggaa tttcatctaa ctttttgagt gaaactatca aataataatt 960
ttaaaacaat tttataagag aagctccgga gataaaaggg catctaatct atgttagaag 1020
agtgaagttt actccctctg tcccaaaaat agaattctaa gtatgaaatg atttttttgt 1080
tatacgaaag gagtatatat cacaagattg atgtcagtta tgcttagggc acgtacacga 1140
cgctggtgct ttaggtagac gttaatcgtt gtttctgcat tttattttat tttgttgcca 1200
cggtgtacat ttgggtagac gtttgtcaca ggcattgcca ctcaaacaag cagccggcgc 1260
ttggagcttt tatagtttga aaagtgacgg ttttaatgat gggtaagctg attagtatat 1320
gtaagtttag ctttttccat tgtaggttaa gccttaaggc tcttacacaa ttgtttcatt 1380
attctcattc tttaagagcc catataagcg ttcatgaatt gtacatatcc ttagatgttt 1440
ttttttttgg gtaaagctcg agcttctcta tctaaaagta gagaaatcag aaaaagattc 1500
atgttttggt agttttgatt tcttgcctcc ataataattt tggtttacca ttttttgttt 1560
gattttagtt ttagaagcgt ttatagcagg atttaaaatc caaaactacc attatcttca 1620
agtgaccgtc agtgagccgt ttaacggcgt cgacaagtcc aacggacacc aaccagtgaa 1680
ccaccagcgt cgagccaagc gatgcaaacg gaacggccga gacgttgaca cctttggcgc 1740
ggcacggcat gtcggatctc cctctctggc cagagagttc cagctccacc tccacctcca 1800
cctccaccgg tggcggtttc caagtccgtt ccgttccgtt ccgttccgtt ccgttccgcc 1860
tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc accggcagca cggggggatt 1920
ccttttccac tgctccttcc tcttcccttc ctcgcccgcc gctataaata gccagccccg 1980
tccccagatt ctttcccaac ctcatctttg ttcggagcac gcacacaacc cgatccccaa 2040
ttccctcgtc tctcctcgcg agcctcgtcg acccccccct tcaaggtacg gcgatcatcc 2100
tccctccctc cctctctcta ccttctcttc tctagactag atcggcgacc cggtccatgg 2160
ttagggcctg ctagttctgt tcctgttttt tccatggctg cgaggtaaaa tagatctgat 2220
ggcgttatga tggttaactc gtcatactct tgcgatctat ggtcccttta ggacatcgat 2280
ttaatttcgg atggttcgag atcggtgatc catggttagt accctaggca gtggggttag 2340
atccgtgctg ttagggttcg tagatggatt ctgattgctc agtaactggg aaacctggga 2400
tggttctagc tgggaatcct gggatggttc tagctggttc gcagatgaga tcgatttcat 2460
ggtctgctat atcttgtttc gttgcctagg ttccgtttaa tctgtccgtg gtatgatgtt 2520
agcctttgat aaggttcgat cgtgctagct acgtcctgcg cagcatttaa ttgtcaggtc 2580
ataattttta gcattcctgt ttttgtttgg tttggttttg tctggttggg ctgtagatag 2640
tttcaatcta cctgtcggtt tattttatta aatttggatt ggatctgtat gtgtcacata 2700
tatcttcatg attaagatgg ttggaattat ctcttcatct tttagatata tatggatagg 2760
tatatatgtt gctgtgggtt ttactggtac tttattagat atattcatgc ttagatacat 2820
gaagcaacgt gctgttacag tttaataatt cttgtttatc taataaacaa ataaggatag 2880
gtatatgttg ctgatggttt tactgatact ttattagata gtactttgac atgaaggaac 2940
atcctgcgac agcttaataa ttattcttca tctaataaaa agcttgcttt ttaattattt 3000
taattatttt gatatacttg gatgatgtca tgcagcagct atgtgtgaat tttcggccct 3060
gtcttcatat gatgtttatt tgcttgggac tgtttctttg gctgataact taccctgttg 3120
tttggtgatc cttctgcagg tg 3142
<210> 39
<211> 1999
<212> DNA
<213> Bouteloua gracilis
<400> 39
gaatcccggc atggcatgtt agaccggagt gagccggccc ttttactggt atgacactcc 60
ctctgtcttg agtgtcgctg tgccagcttg tacctctgtc tatgttcaca gcccgtgctg 120
tgtacctaga ccctccgttt gtccacattc attttaatct ctattgtatc ttgtcaaaac 180
ctaaaagcct aaaacgactc tgataaaggg acagaaagat tatacaagag caagtgtata 240
atgaaataat gtaagcgagc tatatgaatt gtcacgtgtc atatttatgt tgagacgaag 300
aagagaaaat aaacaccatg caaatttatg gcgagtgata gatggccaga tgggcacaag 360
gcctcctatt tcttaaatcg gattttgtaa gaacgaaaaa agggacttat aagagaatag 420
gatagaccat atatcaatga tgtagtatgc atcaagatct aactattata tgagtgaatt 480
gataaattta ttctaggtga catggcctta acgatgaaca gtacgtggtt aaatcaatag 540
aacaatagcc aactctagcg gctctaaaaa aagatatata ttcgtcgagg cactattatg 600
caaccacata gtcaacttca acgccgcttg agtgcgttct catgtttttt ttttcttgca 660
aattacgctt ttctaaaata aaataatttg gatcgtgcaa ttatttcact ttaggtgtgc 720
gtgactacgt gagtaacaat tttgaatctc agaaaggaaa taaaagtata atactgctac 780
ctactttgag gattcagctt gttacttaaa accgtcttta aggtcaaatg ctcaagattc 840
attcaacaat tgaaacgtct cacatgatta aaccatgtat aaggatgcta aggtcttgct 900
tgacaatgtt tttctaggaa tttcatctaa ctttttgagt gaaactatca aataataatt 960
ttaaaacaat tttataagag aagctccgga gataaaaggg catctaatct atgttagaag 1020
agtgaagttt actccctctg tcccaaaaat agaattctaa gtatgaaatg atttttttgt 1080
tatacgaaag gagtatatat cacaagattg atgtcagtta tgcttagggc acgtacacga 1140
cgctggtgct ttaggtagac gttaatcgtt gtttctgcat tttattttat tttgttgcca 1200
cggtgtacat ttgggtagac gtttgtcaca ggcattgcca ctcaaacaag cagccggcgc 1260
ttggagcttt tatagtttga aaagtgacgg ttttaatgat gggtaagctg attagtatat 1320
gtaagtttag ctttttccat tgtaggttaa gccttaaggc tcttacacaa ttgtttcatt 1380
attctcattc tttaagagcc catataagcg ttcatgaatt gtacatatcc ttagatgttt 1440
ttttttttgg gtaaagctcg agcttctcta tctaaaagta gagaaatcag aaaaagattc 1500
atgttttggt agttttgatt tcttgcctcc ataataattt tggtttacca ttttttgttt 1560
gattttagtt ttagaagcgt ttatagcagg atttaaaatc caaaactacc attatcttca 1620
agtgaccgtc agtgagccgt ttaacggcgt cgacaagtcc aacggacacc aaccagtgaa 1680
ccaccagcgt cgagccaagc gatgcaaacg gaacggccga gacgttgaca cctttggcgc 1740
ggcacggcat gtcggatctc cctctctggc cagagagttc cagctccacc tccacctcca 1800
cctccaccgg tggcggtttc caagtccgtt ccgttccgtt ccgttccgtt ccgttccgcc 1860
tcctgcctgc tcctctcaga cggcacgaaa ccgtgacggc accggcagca cggggggatt 1920
ccttttccac tgctccttcc tcttcccttc ctcgcccgcc gctataaata gccagccccg 1980
tccccagatt ctttcccaa 1999
<210> 40
<211> 1057
<212> DNA
<213> Bouteloua gracilis
<400> 40
gtacggcgat catcctccct ccctccctct ctctaccttc tcttctctag actagatcgg 60
cgacccggtc catggttagg gcctgctagt tctgttcctg ttttttccat ggctgcgagg 120
taaaatagat ctgatggcgt tatgatggtt aactcgtcat actcttgcga tctatggtcc 180
ctttaggaca tcgatttaat ttcggatggt tcgagatcgg tgatccatgg ttagtaccct 240
aggcagtggg gttagatccg tgctgttagg gttcgtagat ggattctgat tgctcagtaa 300
ctgggaaacc tgggatggtt ctagctggga atcctgggat ggttctagct ggttcgcaga 360
tgagatcgat ttcatggtct gctatatctt gtttcgttgc ctaggttccg tttaatctgt 420
ccgtggtatg atgttagcct ttgataaggt tcgatcgtgc tagctacgtc ctgcgcagca 480
tttaattgtc aggtcataat ttttagcatt cctgtttttg tttggtttgg ttttgtctgg 540
ttgggctgta gatagtttca atctacctgt cggtttattt tattaaattt ggattggatc 600
tgtatgtgtc acatatatct tcatgattaa gatggttgga attatctctt catcttttag 660
atatatatgg ataggtatat atgttgctgt gggttttact ggtactttat tagatatatt 720
catgcttaga tacatgaagc aacgtgctgt tacagtttaa taattcttgt ttatctaata 780
aacaaataag gataggtata tgttgctgat ggttttactg atactttatt agatagtact 840
ttgacatgaa ggaacatcct gcgacagctt aataattatt cttcatctaa taaaaagctt 900
gctttttaat tattttaatt attttgatat acttggatga tgtcatgcag cagctatgtg 960
tgaattttcg gccctgtctt catatgatgt ttatttgctt gggactgttt ctttggctga 1020
taacttaccc tgttgtttgg tgatccttct gcaggtg 1057
<210> 41
<211> 2165
<212> DNA
<213> Bouteloua gracilis
<400> 41
gagaagctcc ggagataaaa gggcatctaa tctatgttag aagagtgaag tttactccct 60
ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacga aaggagtata 120
tatcacaaga ttgatgtcag ttatgcttag ggcacgtaca cgacgctggt gctttaggta 180
gacgttaatc gttgtttctg cattttattt tattttgttg ccacggtgta catttgggta 240
gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt 300
tgaaaagtga cggttttaat gatgggtaag ctgattagta tatgtaagtt tagctttttc 360
cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga 420
gcccatataa gcgttcatga attgtacata tccttagatg tttttttttt tgggtaaagc 480
tcgagcttct ctatctaaaa gtagagaaat cagaaaaaga ttcatgtttt ggtagttttg 540
atttcttgcc tccataataa ttttggttta ccattttttg tttgatttta gttttagaag 600
cgtttatagc aggatttaaa atccaaaact accattatct tcaagtgacc gtcagtgagc 660
cgtttaacgg cgtcgacaag tccaacggac accaaccagt gaaccaccag cgtcgagcca 720
agcgatgcaa acggaacggc cgagacgttg acacctttgg cgcggcacgg catgtcggat 780
ctccctctct ggccagagag ttccagctcc acctccacct ccacctccac cggtggcggt 840
ttccaagtcc gttccgttcc gttccgttcc gttccgttcc gcctcctgcc tgctcctctc 900
agacggcacg aaaccgtgac ggcaccggca gcacgggggg attccttttc cactgctcct 960
tcctcttccc ttcctcgccc gccgctataa atagccagcc ccgtccccag attctttccc 1020
aacctcatct ttgttcggag cacgcacaca acccgatccc caattccctc gtctctcctc 1080
gcgagcctcg tcgacccccc ccttcaaggt acggcgatca tcctccctcc ctccctctct 1140
ctaccttctc ttctctagac tagatcggcg acccggtcca tggttagggc ctgctagttc 1200
tgttcctgtt ttttccatgg ctgcgaggta aaatagatct gatggcgtta tgatggttaa 1260
ctcgtcatac tcttgcgatc tatggtccct ttaggacatc gatttaattt cggatggttc 1320
gagatcggtg atccatggtt agtaccctag gcagtggggt tagatccgtg ctgttagggt 1380
tcgtagatgg attctgattg ctcagtaact gggaaacctg ggatggttct agctgggaat 1440
cctgggatgg ttctagctgg ttcgcagatg agatcgattt catggtctgc tatatcttgt 1500
ttcgttgcct aggttccgtt taatctgtcc gtggtatgat gttagccttt gataaggttc 1560
gatcgtgcta gctacgtcct gcgcagcatt taattgtcag gtcataattt ttagcattcc 1620
tgtttttgtt tggtttggtt ttgtctggtt gggctgtaga tagtttcaat ctacctgtcg 1680
gtttatttta ttaaatttgg attggatctg tatgtgtcac atatatcttc atgattaaga 1740
tggttggaat tatctcttca tcttttagat atatatggat aggtatatat gttgctgtgg 1800
gttttactgg tactttatta gatatattca tgcttagata catgaagcaa cgtgctgtta 1860
cagtttaata attcttgttt atctaataaa caaataagga taggtatatg ttgctgatgg 1920
ttttactgat actttattag atagtacttt gacatgaagg aacatcctgc gacagcttaa 1980
taattattct tcatctaata aaaagcttgc tttttaatta ttttaattat tttgatatac 2040
ttggatgatg tcatgcagca gctatgtgtg aattttcggc cctgtcttca tatgatgttt 2100
atttgcttgg gactgtttct ttggctgata acttaccctg ttgtttggtg atccttctgc 2160
aggtg 2165
<210> 42
<211> 1022
<212> DNA
<213> Bouteloua gracilis
<400> 42
gagaagctcc ggagataaaa gggcatctaa tctatgttag aagagtgaag tttactccct 60
ctgtcccaaa aatagaattc taagtatgaa atgatttttt tgttatacga aaggagtata 120
tatcacaaga ttgatgtcag ttatgcttag ggcacgtaca cgacgctggt gctttaggta 180
gacgttaatc gttgtttctg cattttattt tattttgttg ccacggtgta catttgggta 240
gacgtttgtc acaggcattg ccactcaaac aagcagccgg cgcttggagc ttttatagtt 300
tgaaaagtga cggttttaat gatgggtaag ctgattagta tatgtaagtt tagctttttc 360
cattgtaggt taagccttaa ggctcttaca caattgtttc attattctca ttctttaaga 420
gcccatataa gcgttcatga attgtacata tccttagatg tttttttttt tgggtaaagc 480
tcgagcttct ctatctaaaa gtagagaaat cagaaaaaga ttcatgtttt ggtagttttg 540
atttcttgcc tccataataa ttttggttta ccattttttg tttgatttta gttttagaag 600
cgtttatagc aggatttaaa atccaaaact accattatct tcaagtgacc gtcagtgagc 660
cgtttaacgg cgtcgacaag tccaacggac accaaccagt gaaccaccag cgtcgagcca 720
agcgatgcaa acggaacggc cgagacgttg acacctttgg cgcggcacgg catgtcggat 780
ctccctctct ggccagagag ttccagctcc acctccacct ccacctccac cggtggcggt 840
ttccaagtcc gttccgttcc gttccgttcc gttccgttcc gcctcctgcc tgctcctctc 900
agacggcacg aaaccgtgac ggcaccggca gcacgggggg attccttttc cactgctcct 960
tcctcttccc ttcctcgccc gccgctataa atagccagcc ccgtccccag attctttccc 1020
aa 1022
<210> 43
<211> 1903
<212> DNA
<213> Bouteloua gracilis
<400> 43
actcaaacaa gcagccggcg cttggagctt ttatagtttg aaaagtgacg gttttaatga 60
tgggtaagct gattagtata tgtaagttta gctttttcca ttgtaggtta agccttaagg 120
ctcttacaca attgtttcat tattctcatt ctttaagagc ccatataagc gttcatgaat 180
tgtacatatc cttagatgtt tttttttttg ggtaaagctc gagcttctct atctaaaagt 240
agagaaatca gaaaaagatt catgttttgg tagttttgat ttcttgcctc cataataatt 300
ttggtttacc attttttgtt tgattttagt tttagaagcg tttatagcag gatttaaaat 360
ccaaaactac cattatcttc aagtgaccgt cagtgagccg tttaacggcg tcgacaagtc 420
caacggacac caaccagtga accaccagcg tcgagccaag cgatgcaaac ggaacggccg 480
agacgttgac acctttggcg cggcacggca tgtcggatct ccctctctgg ccagagagtt 540
ccagctccac ctccacctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt 600
tccgttccgt tccgttccgc ctcctgcctg ctcctctcag acggcacgaa accgtgacgg 660
caccggcagc acggggggat tccttttcca ctgctccttc ctcttccctt cctcgcccgc 720
cgctataaat agccagcccc gtccccagat tctttcccaa cctcatcttt gttcggagca 780
cgcacacaac ccgatcccca attccctcgt ctctcctcgc gagcctcgtc gacccccccc 840
ttcaaggtac ggcgatcatc ctccctccct ccctctctct accttctctt ctctagacta 900
gatcggcgac ccggtccatg gttagggcct gctagttctg ttcctgtttt ttccatggct 960
gcgaggtaaa atagatctga tggcgttatg atggttaact cgtcatactc ttgcgatcta 1020
tggtcccttt aggacatcga tttaatttcg gatggttcga gatcggtgat ccatggttag 1080
taccctaggc agtggggtta gatccgtgct gttagggttc gtagatggat tctgattgct 1140
cagtaactgg gaaacctggg atggttctag ctgggaatcc tgggatggtt ctagctggtt 1200
cgcagatgag atcgatttca tggtctgcta tatcttgttt cgttgcctag gttccgttta 1260
atctgtccgt ggtatgatgt tagcctttga taaggttcga tcgtgctagc tacgtcctgc 1320
gcagcattta attgtcaggt cataattttt agcattcctg tttttgtttg gtttggtttt 1380
gtctggttgg gctgtagata gtttcaatct acctgtcggt ttattttatt aaatttggat 1440
tggatctgta tgtgtcacat atatcttcat gattaagatg gttggaatta tctcttcatc 1500
ttttagatat atatggatag gtatatatgt tgctgtgggt tttactggta ctttattaga 1560
tatattcatg cttagataca tgaagcaacg tgctgttaca gtttaataat tcttgtttat 1620
ctaataaaca aataaggata ggtatatgtt gctgatggtt ttactgatac tttattagat 1680
agtactttga catgaaggaa catcctgcga cagcttaata attattcttc atctaataaa 1740
aagcttgctt tttaattatt ttaattattt tgatatactt ggatgatgtc atgcagcagc 1800
tatgtgtgaa ttttcggccc tgtcttcata tgatgtttat ttgcttggga ctgtttcttt 1860
ggctgataac ttaccctgtt gtttggtgat ccttctgcag gtg 1903
<210> 44
<211> 760
<212> DNA
<213> Bouteloua gracilis
<400> 44
actcaaacaa gcagccggcg cttggagctt ttatagtttg aaaagtgacg gttttaatga 60
tgggtaagct gattagtata tgtaagttta gctttttcca ttgtaggtta agccttaagg 120
ctcttacaca attgtttcat tattctcatt ctttaagagc ccatataagc gttcatgaat 180
tgtacatatc cttagatgtt tttttttttg ggtaaagctc gagcttctct atctaaaagt 240
agagaaatca gaaaaagatt catgttttgg tagttttgat ttcttgcctc cataataatt 300
ttggtttacc attttttgtt tgattttagt tttagaagcg tttatagcag gatttaaaat 360
ccaaaactac cattatcttc aagtgaccgt cagtgagccg tttaacggcg tcgacaagtc 420
caacggacac caaccagtga accaccagcg tcgagccaag cgatgcaaac ggaacggccg 480
agacgttgac acctttggcg cggcacggca tgtcggatct ccctctctgg ccagagagtt 540
ccagctccac ctccacctcc acctccaccg gtggcggttt ccaagtccgt tccgttccgt 600
tccgttccgt tccgttccgc ctcctgcctg ctcctctcag acggcacgaa accgtgacgg 660
caccggcagc acggggggat tccttttcca ctgctccttc ctcttccctt cctcgcccgc 720
cgctataaat agccagcccc gtccccagat tctttcccaa 760
<210> 45
<211> 3234
<212> DNA
<213> Bouteloua gracilis
<400> 45
ggcctcttta cgtttggcac aacttagttg aatccggctt ccggcaaact atatggcaag 60
ttagacccaa gtgtgagccg gccaccgcaa gttattgtga cattatacgt aggaagcaag 120
tgtataataa gaatatgaga taatgtaagc agctatatga attatcacgt catatttatg 180
ttaagatgaa gaggagagaa taaacggtac gtaaatttat agcgagtgat agacgggcac 240
gaggcctcct agctatttcc ataaatcgga ttttgtaaga acaaaaaaga ggacttatta 300
taagagaatg tggtaagtaa gcatactccc tccgtttcaa attataagtt gttttaactt 360
tttttttata tctattttac tatacattag atataataat gtgtctagat acataataaa 420
atggatgaac aaaaaagtca aagtgactta caatttggaa cggagggagt aagttcaagc 480
catcaaggca cttctatgca accacatagt caacttgaat gccgcttgag tgccttctca 540
agtttttttt ttcttgcaaa aattgtttct ttttttttaa aaaagtataa tttggatcgt 600
gcaaatttct ctctaggtgt gtgtgtgact gtgtgagtaa caatttctct agttgtgcgt 660
gactgctgct tactttggag attacaatat atttctaaaa tgcttcgatt acttatttat 720
aaaccgtctc taaggccaat tgctcaagat tcattcaaca attgaaacgt ctcacatgat 780
taaatcatat aaagtttcta agtcttgttt gacaagattt ttttagattt tcatctaaat 840
tggatgaaac tatcaaacac taattttaaa aaatataaga gaagctccgg agataaaagg 900
tcgtctatgt tattataaga gtaaagtcgt ctattctctt cgtcccaaca tatataattc 960
taagcatgaa ttgctttctt tttggacaaa aggagtatgc cacaacacaa gaatgatgtc 1020
accgtcatgc ttagatcctt ttatggtaaa gcttcacctt ctataatcta acaatagaga 1080
aatcggggaa aaatcatgtt ttggttgttt ttatttctaa cctccacaat aactttggtt 1140
taccattttt tgtttgattt tagttttaga gaagcgttta taacaggacc taaaatcttt 1200
ttttgagtac acagtacaac gcagacgctc atacacgcac gcacaatgtc ctctatgaac 1260
acacgtaagg aaaccctaca ccttgagcac cttcgaagga ctgagccggc aaatctagag 1320
attctcgaag tcactattgg cacctcgtta tcaacgagaa cgtcgcttac cacttaaagc 1380
ataacaccga gaaatcccgt aacaaatcca gtaaaatacg agcacccgta ccaagttgaa 1440
tatttgaacc cgagtgggta gattccaccg caaaggacct aaccagatca tttcgcaaac 1500
aggaactaaa atcggtagag agcccagaca aaaacctttt ctaagagcaa ctccagtgaa 1560
agcccctact ttaggtataa aatgcaacac tagtggagct tctaaataaa cttctatttt 1620
tcatgccctc ctaaaattta ctcctaaaac cctagctata ggagcctcct atccatcctc 1680
tattttattc cactagaatt gattataaat ttagcctctt aaattttata agttgggagt 1740
cgagggtaac tagagttgct ctaaacggac cttatcttca agtgacctca gtgagcccgt 1800
ttaacggcgt cgacaagtct aatctaacgg acaccaacca gagaaccacc gccagcgccg 1860
agccaagcga cgttgacatc ttggcgcggc acggcatctc cctggcgtct ggtcccctcc 1920
cgagacttcc gctccacctc ccaccggtgg cggtttccga gtccgttccg cctcctctca 1980
cacggcacga aaccttgacg gcaccggcag cacgggggat tccgttccca cggctccttc 2040
cctttccctt cctcgcccgc tgctataaat agccagcccc atccccagct tcttccccaa 2100
cctcatcttc tcgtgttgtt cggcccaacc cgatcgatcc ccaattccct cgtcgtctct 2160
cgtcgcgagc ctcgtcgatc cccgcttcaa ggtacagcga tcgatcgatc atcctcgctc 2220
tctctacctt ctctctctta gggcgtgctg gttctgttcc tgtttttcca tggctgcgag 2280
gtacaataga ttggcgattc atggttaggg cctgctagtt ctgttcctgt tttttttttt 2340
tccatggctg cgaggcacaa tagatctgat ggcgttatga tggttaactt gtcatactct 2400
tgcgatctat ggtcccttta ggagtttagg acatcgattt aatttcggat agttcgagat 2460
ctgtgatcca tggttagtac cctaggcagt ggggttagat ccgtgctgtt atggttcgta 2520
gatggattct gattgctcag taactgggaa tcctgggatg gttctagctg gttcgcagat 2580
aagatcgatt tcatgatatg ctatatcttg tttggttgcc gtggttccgt taaatctgtc 2640
tgttatgatc ttagtctttg ataaggttcg gtcgtgctag ctacgtcctg tgcagcactt 2700
aattgtcagg tcataatttt tagcatgcct tttttttatt ggtttggttt tgtctgactg 2760
ggctgtagat agtttcaatc tttgtctgac tgggctgtag atagtttcaa tcttcctgtc 2820
tgtttatttt attaaatttg gatctgtatg tgtgtcatat atcttcatct tttagatata 2880
tcgataggta tatatgttgc tgtcgttttt tactgttcct ttatgagata tattcatgct 2940
tagatacatg aaacaacgtg ctgttacagt ttaatagttc ttgtttatct aataaacaaa 3000
taaggatagg tgctgcagtt agttttactg gtactttttt tgacatgaac ctacggctta 3060
ataattagtc ttcatcaaat aaaaagcata ttttttaatt atttcgatat acttgaatga 3120
tgtcatatgc agcatctgtg tgaatttttg gccctgtctt catatgatgt ttatttgctt 3180
gggactgttt ctttggctga taactcaccc tgttgtttgg tgatccttct gcag 3234
<210> 46
<211> 2100
<212> DNA
<213> Bouteloua gracilis
<400> 46
ggcctcttta cgtttggcac aacttagttg aatccggctt ccggcaaact atatggcaag 60
ttagacccaa gtgtgagccg gccaccgcaa gttattgtga cattatacgt aggaagcaag 120
tgtataataa gaatatgaga taatgtaagc agctatatga attatcacgt catatttatg 180
ttaagatgaa gaggagagaa taaacggtac gtaaatttat agcgagtgat agacgggcac 240
gaggcctcct agctatttcc ataaatcgga ttttgtaaga acaaaaaaga ggacttatta 300
taagagaatg tggtaagtaa gcatactccc tccgtttcaa attataagtt gttttaactt 360
tttttttata tctattttac tatacattag atataataat gtgtctagat acataataaa 420
atggatgaac aaaaaagtca aagtgactta caatttggaa cggagggagt aagttcaagc 480
catcaaggca cttctatgca accacatagt caacttgaat gccgcttgag tgccttctca 540
agtttttttt ttcttgcaaa aattgtttct ttttttttaa aaaagtataa tttggatcgt 600
gcaaatttct ctctaggtgt gtgtgtgact gtgtgagtaa caatttctct agttgtgcgt 660
gactgctgct tactttggag attacaatat atttctaaaa tgcttcgatt acttatttat 720
aaaccgtctc taaggccaat tgctcaagat tcattcaaca attgaaacgt ctcacatgat 780
taaatcatat aaagtttcta agtcttgttt gacaagattt ttttagattt tcatctaaat 840
tggatgaaac tatcaaacac taattttaaa aaatataaga gaagctccgg agataaaagg 900
tcgtctatgt tattataaga gtaaagtcgt ctattctctt cgtcccaaca tatataattc 960
taagcatgaa ttgctttctt tttggacaaa aggagtatgc cacaacacaa gaatgatgtc 1020
accgtcatgc ttagatcctt ttatggtaaa gcttcacctt ctataatcta acaatagaga 1080
aatcggggaa aaatcatgtt ttggttgttt ttatttctaa cctccacaat aactttggtt 1140
taccattttt tgtttgattt tagttttaga gaagcgttta taacaggacc taaaatcttt 1200
ttttgagtac acagtacaac gcagacgctc atacacgcac gcacaatgtc ctctatgaac 1260
acacgtaagg aaaccctaca ccttgagcac cttcgaagga ctgagccggc aaatctagag 1320
attctcgaag tcactattgg cacctcgtta tcaacgagaa cgtcgcttac cacttaaagc 1380
ataacaccga gaaatcccgt aacaaatcca gtaaaatacg agcacccgta ccaagttgaa 1440
tatttgaacc cgagtgggta gattccaccg caaaggacct aaccagatca tttcgcaaac 1500
aggaactaaa atcggtagag agcccagaca aaaacctttt ctaagagcaa ctccagtgaa 1560
agcccctact ttaggtataa aatgcaacac tagtggagct tctaaataaa cttctatttt 1620
tcatgccctc ctaaaattta ctcctaaaac cctagctata ggagcctcct atccatcctc 1680
tattttattc cactagaatt gattataaat ttagcctctt aaattttata agttgggagt 1740
cgagggtaac tagagttgct ctaaacggac cttatcttca agtgacctca gtgagcccgt 1800
ttaacggcgt cgacaagtct aatctaacgg acaccaacca gagaaccacc gccagcgccg 1860
agccaagcga cgttgacatc ttggcgcggc acggcatctc cctggcgtct ggtcccctcc 1920
cgagacttcc gctccacctc ccaccggtgg cggtttccga gtccgttccg cctcctctca 1980
cacggcacga aaccttgacg gcaccggcag cacgggggat tccgttccca cggctccttc 2040
cctttccctt cctcgcccgc tgctataaat agccagcccc atccccagct tcttccccaa 2100
<210> 47
<211> 91
<212> DNA
<213> Bouteloua gracilis
<400> 47
cctcatcttc tcgtgttgtt cggcccaacc cgatcgatcc ccaattccct cgtcgtctct 60
cgtcgcgagc ctcgtcgatc cccgcttcaa g 91
<210> 48
<211> 1043
<212> DNA
<213> Bouteloua gracilis
<400> 48
gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 60
ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 120
ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg 180
gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga 240
catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg 300
gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat 360
cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt 420
ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtctttga taaggttcgg 480
tcgtgctagc tacgtcctgt gcagcactta attgtcaggt cataattttt agcatgcctt 540
ttttttattg gtttggtttt gtctgactgg gctgtagata gtttcaatct ttgtctgact 600
gggctgtaga tagtttcaat cttcctgtct gtttatttta ttaaatttgg atctgtatgt 660
gtgtcatata tcttcatctt ttagatatat cgataggtat atatgttgct gtcgtttttt 720
actgttcctt tatgagatat attcatgctt agatacatga aacaacgtgc tgttacagtt 780
taatagttct tgtttatcta ataaacaaat aaggataggt gctgcagtta gttttactgg 840
tacttttttt gacatgaacc tacggcttaa taattagtct tcatcaaata aaaagcatat 900
tttttaatta tttcgatata cttgaatgat gtcatatgca gcatctgtgt gaatttttgg 960
ccctgtcttc atatgatgtt tatttgcttg ggactgtttc tttggctgat aactcaccct 1020
gttgtttggt gatccttctg cag 1043
<210> 49
<211> 3176
<212> DNA
<213> Bouteloua gracilis
<400> 49
aagttagacc caagtgtgag ccggccaccg caagttattg tgacattata cgtaggaagc 60
aagtgtataa taagaatatg agataatgta agcagctata tgaattatca cgtcatattt 120
atgttaagat gaagaggaga gaataaacgg tacgtaaatt tatagcgagt gatagacggg 180
cacgaggcct cctagctatt tccataaatc ggattttgta agaacaaaaa agaggactta 240
ttataagaga atgtggtaag taagcatact ccctccgttt caaattataa gttgttttaa 300
cttttttttt atatctattt tactatacat tagatataat aatgtgtcta gatacataat 360
aaaatggatg aacaaaaaag tcaaagtgac ttacaatttg gaacggaggg agtaagttca 420
agccatcaag gcacttctat gcaaccacat agtcaacttg aatgccgctt gagtgccttc 480
tcaagttttt tttttcttgc aaaaattgtt tctttttttt taaaaaagta taatttggat 540
cgtgcaaatt tctctctagg tgtgtgtgtg actgtgtgag taacaatttc tctagttgtg 600
cgtgactgct gcttactttg gagattacaa tatatttcta aaatgcttcg attacttatt 660
tataaaccgt ctctaaggcc aattgctcaa gattcattca acaattgaaa cgtctcacat 720
gattaaatca tataaagttt ctaagtcttg tttgacaaga tttttttaga ttttcatcta 780
aattggatga aactatcaaa cactaatttt aaaaaatata agagaagctc cggagataaa 840
aggtcgtcta tgttattata agagtaaagt cgtctattct cttcgtccca acatatataa 900
ttctaagcat gaattgcttt ctttttggac aaaaggagta tgccacaaca caagaatgat 960
gtcaccgtca tgcttagatc cttttatggt aaagcttcac cttctataat ctaacaatag 1020
agaaatcggg gaaaaatcat gttttggttg tttttatttc taacctccac aataactttg 1080
gtttaccatt ttttgtttga ttttagtttt agagaagcgt ttataacagg acctaaaatc 1140
tttttttgag tacacagtac aacgcagacg ctcatacacg cacgcacaat gtcctctatg 1200
aacacacgta aggaaaccct acaccttgag caccttcgaa ggactgagcc ggcaaatcta 1260
gagattctcg aagtcactat tggcacctcg ttatcaacga gaacgtcgct taccacttaa 1320
agcataacac cgagaaatcc cgtaacaaat ccagtaaaat acgagcaccc gtaccaagtt 1380
gaatatttga acccgagtgg gtagattcca ccgcaaagga cctaaccaga tcatttcgca 1440
aacaggaact aaaatcggta gagagcccag acaaaaacct tttctaagag caactccagt 1500
gaaagcccct actttaggta taaaatgcaa cactagtgga gcttctaaat aaacttctat 1560
ttttcatgcc ctcctaaaat ttactcctaa aaccctagct ataggagcct cctatccatc 1620
ctctatttta ttccactaga attgattata aatttagcct cttaaatttt ataagttggg 1680
agtcgagggt aactagagtt gctctaaacg gaccttatct tcaagtgacc tcagtgagcc 1740
cgtttaacgg cgtcgacaag tctaatctaa cggacaccaa ccagagaacc accgccagcg 1800
ccgagccaag cgacgttgac atcttggcgc ggcacggcat ctccctggcg tctggtcccc 1860
tcccgagact tccgctccac ctcccaccgg tggcggtttc cgagtccgtt ccgcctcctc 1920
tcacacggca cgaaaccttg acggcaccgg cagcacgggg gattccgttc ccacggctcc 1980
ttccctttcc cttcctcgcc cgctgctata aatagccagc cccatcccca gcttcttccc 2040
caacctcatc ttctcgtgtt gttcggccca acccgatcga tccccaattc cctcgtcgtc 2100
tctcgtcgcg agcctcgtcg atccccgctt caaggtacag cgatcgatcg atcatcctcg 2160
ctctctctac cttctctctc ttagggcgtg ctggttctgt tcctgttttt ccatggctgc 2220
gaggtacaat agattggcga ttcatggtta gggcctgcta gttctgttcc tgtttttttt 2280
ttttccatgg ctgcgaggca caatagatct gatggcgtta tgatggttaa cttgtcatac 2340
tcttgcgatc tatggtccct ttaggagttt aggacatcga tttaatttcg gatagttcga 2400
gatctgtgat ccatggttag taccctaggc agtggggtta gatccgtgct gttatggttc 2460
gtagatggat tctgattgct cagtaactgg gaatcctggg atggttctag ctggttcgca 2520
gataagatcg atttcatgat atgctatatc ttgtttggtt gccgtggttc cgttaaatct 2580
gtctgttatg atcttagtct tgataaggtt cggtcgtgct agctacgtcc tgtgcagcac 2640
ttaattgtca ggtcataatt tttagcatgc ctttttttta ttggtttggt tttgtctgac 2700
tgggctgtag atagtttcaa tctttgtctg actgggctgt agatagtttc aatcttcctg 2760
tctgtttatt ttattaaatt tggatctgta tgtgtgtcat atatcttcat cttttagata 2820
tatcgatagg tatatatgtt gctgtcgttt tttactgttc ctttatgaga tatattcatg 2880
cttagataca tgaaacaacg tgctgttaca gtttaatagt tcttgtttat ctaataaaca 2940
aataaggata ggtgctgcag ttagttttac tggtactttt tttgacatga acctacggct 3000
taataattag tcttcatcaa ataaaaagca tattttttaa ttatttcgat atacttgaat 3060
gatgtcatat gcagcatctg tgtgaatttt tggccctgtc ttcatatgat gtttatttgc 3120
ttgggactgt ttctttggct gataactcac cctgttgttt ggtgatcctt ctgcag 3176
<210> 50
<211> 2043
<212> DNA
<213> Bouteloua gracilis
<400> 50
aagttagacc caagtgtgag ccggccaccg caagttattg tgacattata cgtaggaagc 60
aagtgtataa taagaatatg agataatgta agcagctata tgaattatca cgtcatattt 120
atgttaagat gaagaggaga gaataaacgg tacgtaaatt tatagcgagt gatagacggg 180
cacgaggcct cctagctatt tccataaatc ggattttgta agaacaaaaa agaggactta 240
ttataagaga atgtggtaag taagcatact ccctccgttt caaattataa gttgttttaa 300
cttttttttt atatctattt tactatacat tagatataat aatgtgtcta gatacataat 360
aaaatggatg aacaaaaaag tcaaagtgac ttacaatttg gaacggaggg agtaagttca 420
agccatcaag gcacttctat gcaaccacat agtcaacttg aatgccgctt gagtgccttc 480
tcaagttttt tttttcttgc aaaaattgtt tctttttttt taaaaaagta taatttggat 540
cgtgcaaatt tctctctagg tgtgtgtgtg actgtgtgag taacaatttc tctagttgtg 600
cgtgactgct gcttactttg gagattacaa tatatttcta aaatgcttcg attacttatt 660
tataaaccgt ctctaaggcc aattgctcaa gattcattca acaattgaaa cgtctcacat 720
gattaaatca tataaagttt ctaagtcttg tttgacaaga tttttttaga ttttcatcta 780
aattggatga aactatcaaa cactaatttt aaaaaatata agagaagctc cggagataaa 840
aggtcgtcta tgttattata agagtaaagt cgtctattct cttcgtccca acatatataa 900
ttctaagcat gaattgcttt ctttttggac aaaaggagta tgccacaaca caagaatgat 960
gtcaccgtca tgcttagatc cttttatggt aaagcttcac cttctataat ctaacaatag 1020
agaaatcggg gaaaaatcat gttttggttg tttttatttc taacctccac aataactttg 1080
gtttaccatt ttttgtttga ttttagtttt agagaagcgt ttataacagg acctaaaatc 1140
tttttttgag tacacagtac aacgcagacg ctcatacacg cacgcacaat gtcctctatg 1200
aacacacgta aggaaaccct acaccttgag caccttcgaa ggactgagcc ggcaaatcta 1260
gagattctcg aagtcactat tggcacctcg ttatcaacga gaacgtcgct taccacttaa 1320
agcataacac cgagaaatcc cgtaacaaat ccagtaaaat acgagcaccc gtaccaagtt 1380
gaatatttga acccgagtgg gtagattcca ccgcaaagga cctaaccaga tcatttcgca 1440
aacaggaact aaaatcggta gagagcccag acaaaaacct tttctaagag caactccagt 1500
gaaagcccct actttaggta taaaatgcaa cactagtgga gcttctaaat aaacttctat 1560
ttttcatgcc ctcctaaaat ttactcctaa aaccctagct ataggagcct cctatccatc 1620
ctctatttta ttccactaga attgattata aatttagcct cttaaatttt ataagttggg 1680
agtcgagggt aactagagtt gctctaaacg gaccttatct tcaagtgacc tcagtgagcc 1740
cgtttaacgg cgtcgacaag tctaatctaa cggacaccaa ccagagaacc accgccagcg 1800
ccgagccaag cgacgttgac atcttggcgc ggcacggcat ctccctggcg tctggtcccc 1860
tcccgagact tccgctccac ctcccaccgg tggcggtttc cgagtccgtt ccgcctcctc 1920
tcacacggca cgaaaccttg acggcaccgg cagcacgggg gattccgttc ccacggctcc 1980
ttccctttcc cttcctcgcc cgctgctata aatagccagc cccatcccca gcttcttccc 2040
caa 2043
<210> 51
<211> 1042
<212> DNA
<213> Bouteloua gracilis
<400> 51
gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 60
ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 120
ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg 180
gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga 240
catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg 300
gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat 360
cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt 420
ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtcttgat aaggttcggt 480
cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt 540
tttttattgg tttggttttg tctgactggg ctgtagatag tttcaatctt tgtctgactg 600
ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg 660
tgtcatatat cttcatcttt tagatatatc gataggtata tatgttgctg tcgtttttta 720
ctgttccttt atgagatata ttcatgctta gatacatgaa acaacgtgct gttacagttt 780
aatagttctt gtttatctaa taaacaaata aggataggtg ctgcagttag ttttactggt 840
actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt 900
ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc 960
cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg 1020
ttgtttggtg atccttctgc ag 1042
<210> 52
<211> 3139
<212> DNA
<213> Bouteloua gracilis
<400> 52
gacattatac gtaggaagca agtgtataat aagaatatga gataatgtaa gcagctatat 60
gaattatcac gtcatattta tgttaagatg aagaggagag aataaacggt acgtaaattt 120
atagcgagtg atagacgggc acgaggcctc ctagctattt ccataaatcg gattttgtaa 180
gaacaaaaaa gaggacttat tataagagaa tgtggtaagt aagcatactc cctccgtttc 240
aaattataag ttgttttaac ttttttttta tatctatttt actatacatt agatataata 300
atgtgtctag atacataata aaatggatga acaaaaaagt caaagtgact tacaatttgg 360
aacggaggga gtaagttcaa gccatcaagg cacttctatg caaccacata gtcaacttga 420
atgccgcttg agtgccttct caagtttttt ttttcttgca aaaattgttt cttttttttt 480
aaaaaagtat aatttggatc gtgcaaattt ctctctaggt gtgtgtgtga ctgtgtgagt 540
aacaatttct ctagttgtgc gtgactgctg cttactttgg agattacaat atatttctaa 600
aatgcttcga ttacttattt ataaaccgtc tctaaggcca attgctcaag attcattcaa 660
caattgaaac gtctcacatg attaaatcat ataaagtttc taagtcttgt ttgacaagat 720
ttttttagat tttcatctaa attggatgaa actatcaaac actaatttta aaaaatataa 780
gagaagctcc ggagataaaa ggtcgtctat gttattataa gagtaaagtc gtctattctc 840
ttcgtcccaa catatataat tctaagcatg aattgctttc tttttggaca aaaggagtat 900
gccacaacac aagaatgatg tcaccgtcat gcttagatcc ttttatggta aagcttcacc 960
ttctataatc taacaataga gaaatcgggg aaaaatcatg ttttggttgt ttttatttct 1020
aacctccaca ataactttgg tttaccattt tttgtttgat tttagtttta gagaagcgtt 1080
tataacagga cctaaaatct ttttttgagt acacagtaca acgcagacgc tcatacacgc 1140
acgcacaatg tcctctatga acacacgtaa ggaaacccta caccttgagc accttcgaag 1200
gactgagccg gcaaatctag agattctcga agtcactatt ggcacctcgt tatcaacgag 1260
aacgtcgctt accacttaaa gcataacacc gagaaatccc gtaacaaatc cagtaaaata 1320
cgagcacccg taccaagttg aatatttgaa cccgagtggg tagattccac cgcaaaggac 1380
ctaaccagat catttcgcaa acaggaacta aaatcggtag agagcccaga caaaaacctt 1440
ttctaagagc aactccagtg aaagccccta ctttaggtat aaaatgcaac actagtggag 1500
cttctaaata aacttctatt tttcatgccc tcctaaaatt tactcctaaa accctagcta 1560
taggagcctc ctatccatcc tctattttat tccactagaa ttgattataa atttagcctc 1620
ttaaatttta taagttggga gtcgagggta actagagttg ctctaaacgg accttatctt 1680
caagtgacct cagtgagccc gtttaacggc gtcgacaagt ctaatctaac ggacaccaac 1740
cagagaacca ccgccagcgc cgagccaagc gacgttgaca tcttggcgcg gcacggcatc 1800
tccctggcgt ctggtcccct cccgagactt ccgctccacc tcccaccggt ggcggtttcc 1860
gagtccgttc cgcctcctct cacacggcac gaaaccttga cggcaccggc agcacggggg 1920
attccgttcc cacggctcct tccctttccc ttcctcgccc gctgctataa atagccagcc 1980
ccatccccag cttcttcccc aacctcatct tctcgtgttg ttcggcccaa cccgatcgat 2040
ccccaattcc ctcgtcgtct ctcgtcgcga gcctcgtcga tccccgcttc aaggtacagc 2100
gatcgatcga tcatcctcgc tctctctacc ttctctctct tagggcgtgc tggttctgtt 2160
cctgtttttc catggctgcg aggtacaata gattggcgat tcatggttag ggcctgctag 2220
ttctgttcct gttttttttt tttccatggc tgcgaggcac aatagatctg atggcgttat 2280
gatggttaac ttgtcatact cttgcgatct atggtccctt taggagttta ggacatcgat 2340
ttaatttcgg atagttcgag atctgtgatc catggttagt accctaggca gtggggttag 2400
atccgtgctg ttatggttcg tagatggatt ctgattgctc agtaactggg aatcctggga 2460
tggttctagc tggttcgcag ataagatcga tttcatgata tgctatatct tgtttggttg 2520
ccgtggttcc gttaaatctg tctgttatga tcttagtctt tgataaggtt cggtcgtgct 2580
agctacgtcc tgtgcagcac ttaattgtca ggtcataatt tttagcatgc ctttttttta 2640
ttggtttggt tttgtctgac tgggctgtag atagtttcaa tctttgtctg actgggctgt 2700
agatagtttc aatcttcctg tctgtttatt ttattaaatt tggatctgta tgtgtgtcat 2760
atatcttcat cttttagata tatcgatagg tatatatgtt gctgtcgttt tttactgttc 2820
ctttatgaga tatattcatg cttagataca tgaaacaacg tgctgttaca gtttaatagt 2880
tcttgtttat ctaataaaca aataaggata ggtgctgcag ttagttttac tggtactttt 2940
tttgacatga acctacggct taataattag tcttcatcaa ataaaaagca tattttttaa 3000
ttatttcgat atacttgaat gatgtcatat gcagcatctg tgtgaatttt tggccctgtc 3060
ttcatatgat gtttatttgc ttgggactgt ttctttggct gataactcac cctgttgttt 3120
ggtgatcctt ctgcaggtg 3139
<210> 53
<211> 2002
<212> DNA
<213> Bouteloua gracilis
<400> 53
gacattatac gtaggaagca agtgtataat aagaatatga gataatgtaa gcagctatat 60
gaattatcac gtcatattta tgttaagatg aagaggagag aataaacggt acgtaaattt 120
atagcgagtg atagacgggc acgaggcctc ctagctattt ccataaatcg gattttgtaa 180
gaacaaaaaa gaggacttat tataagagaa tgtggtaagt aagcatactc cctccgtttc 240
aaattataag ttgttttaac ttttttttta tatctatttt actatacatt agatataata 300
atgtgtctag atacataata aaatggatga acaaaaaagt caaagtgact tacaatttgg 360
aacggaggga gtaagttcaa gccatcaagg cacttctatg caaccacata gtcaacttga 420
atgccgcttg agtgccttct caagtttttt ttttcttgca aaaattgttt cttttttttt 480
aaaaaagtat aatttggatc gtgcaaattt ctctctaggt gtgtgtgtga ctgtgtgagt 540
aacaatttct ctagttgtgc gtgactgctg cttactttgg agattacaat atatttctaa 600
aatgcttcga ttacttattt ataaaccgtc tctaaggcca attgctcaag attcattcaa 660
caattgaaac gtctcacatg attaaatcat ataaagtttc taagtcttgt ttgacaagat 720
ttttttagat tttcatctaa attggatgaa actatcaaac actaatttta aaaaatataa 780
gagaagctcc ggagataaaa ggtcgtctat gttattataa gagtaaagtc gtctattctc 840
ttcgtcccaa catatataat tctaagcatg aattgctttc tttttggaca aaaggagtat 900
gccacaacac aagaatgatg tcaccgtcat gcttagatcc ttttatggta aagcttcacc 960
ttctataatc taacaataga gaaatcgggg aaaaatcatg ttttggttgt ttttatttct 1020
aacctccaca ataactttgg tttaccattt tttgtttgat tttagtttta gagaagcgtt 1080
tataacagga cctaaaatct ttttttgagt acacagtaca acgcagacgc tcatacacgc 1140
acgcacaatg tcctctatga acacacgtaa ggaaacccta caccttgagc accttcgaag 1200
gactgagccg gcaaatctag agattctcga agtcactatt ggcacctcgt tatcaacgag 1260
aacgtcgctt accacttaaa gcataacacc gagaaatccc gtaacaaatc cagtaaaata 1320
cgagcacccg taccaagttg aatatttgaa cccgagtggg tagattccac cgcaaaggac 1380
ctaaccagat catttcgcaa acaggaacta aaatcggtag agagcccaga caaaaacctt 1440
ttctaagagc aactccagtg aaagccccta ctttaggtat aaaatgcaac actagtggag 1500
cttctaaata aacttctatt tttcatgccc tcctaaaatt tactcctaaa accctagcta 1560
taggagcctc ctatccatcc tctattttat tccactagaa ttgattataa atttagcctc 1620
ttaaatttta taagttggga gtcgagggta actagagttg ctctaaacgg accttatctt 1680
caagtgacct cagtgagccc gtttaacggc gtcgacaagt ctaatctaac ggacaccaac 1740
cagagaacca ccgccagcgc cgagccaagc gacgttgaca tcttggcgcg gcacggcatc 1800
tccctggcgt ctggtcccct cccgagactt ccgctccacc tcccaccggt ggcggtttcc 1860
gagtccgttc cgcctcctct cacacggcac gaaaccttga cggcaccggc agcacggggg 1920
attccgttcc cacggctcct tccctttccc ttcctcgccc gctgctataa atagccagcc 1980
ccatccccag cttcttcccc aa 2002
<210> 54
<211> 1046
<212> DNA
<213> Bouteloua gracilis
<400> 54
gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 60
ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 120
ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg 180
gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga 240
catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg 300
gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat 360
cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt 420
ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtctttga taaggttcgg 480
tcgtgctagc tacgtcctgt gcagcactta attgtcaggt cataattttt agcatgcctt 540
ttttttattg gtttggtttt gtctgactgg gctgtagata gtttcaatct ttgtctgact 600
gggctgtaga tagtttcaat cttcctgtct gtttatttta ttaaatttgg atctgtatgt 660
gtgtcatata tcttcatctt ttagatatat cgataggtat atatgttgct gtcgtttttt 720
actgttcctt tatgagatat attcatgctt agatacatga aacaacgtgc tgttacagtt 780
taatagttct tgtttatcta ataaacaaat aaggataggt gctgcagtta gttttactgg 840
tacttttttt gacatgaacc tacggcttaa taattagtct tcatcaaata aaaagcatat 900
tttttaatta tttcgatata cttgaatgat gtcatatgca gcatctgtgt gaatttttgg 960
ccctgtcttc atatgatgtt tatttgcttg ggactgtttc tttggctgat aactcaccct 1020
gttgtttggt gatccttctg caggtg 1046
<210> 55
<211> 2160
<212> DNA
<213> Bouteloua gracilis
<400> 55
gagaaatcgg ggaaaaatca tgttttggtt gtttttattt ctaacctcca caataacttt 60
ggtttaccat tttttgtttg attttagttt tagagaagcg tttataacag gacctaaaat 120
ctttttttga gtacacagta caacgcagac gctcatacac gcacgcacaa tgtcctctat 180
gaacacacgt aaggaaaccc tacaccttga gcaccttcga aggactgagc cggcaaatct 240
agagattctc gaagtcacta ttggcacctc gttatcaacg agaacgtcgc ttaccactta 300
aagcataaca ccgagaaatc ccgtaacaaa tccagtaaaa tacgagcacc cgtaccaagt 360
tgaatatttg aacccgagtg ggtagattcc accgcaaagg acctaaccag atcatttcgc 420
aaacaggaac taaaatcggt agagagccca gacaaaaacc ttttctaaga gcaactccag 480
tgaaagcccc tactttaggt ataaaatgca acactagtgg agcttctaaa taaacttcta 540
tttttcatgc cctcctaaaa tttactccta aaaccctagc tataggagcc tcctatccat 600
cctctatttt attccactag aattgattat aaatttagcc tcttaaattt tataagttgg 660
gagtcgaggg taactagagt tgctctaaac ggaccttatc ttcaagtgac ctcagtgagc 720
ccgtttaacg gcgtcgacaa gtctaatcta acggacacca accagagaac caccgccagc 780
gccgagccaa gcgacgttga catcttggcg cggcacggca tctccctggc gtctggtccc 840
ctcccgagac ttccgctcca cctcccaccg gtggcggttt ccgagtccgt tccgcctcct 900
ctcacacggc acgaaacctt gacggcaccg gcagcacggg ggattccgtt cccacggctc 960
cttccctttc ccttcctcgc ccgctgctat aaatagccag ccccatcccc agcttcttcc 1020
ccaacctcat cttctcgtgt tgttcggccc aacccgatcg atccccaatt ccctcgtcgt 1080
ctctcgtcgc gagcctcgtc gatccccgct tcaaggtaca gcgatcgatc gatcatcctc 1140
gctctctcta ccttctctct cttagggcgt gctggttctg ttcctgtttt tccatggctg 1200
cgaggtacaa tagattggcg attcatggtt agggcctgct agttctgttc ctgttttttt 1260
ttttccatgg ctgcgaggca caatagatct gatggcgtta tgatggttaa cttgtcatac 1320
tcttgcgatc tatggtccct ttaggagttt aggacatcga tttaatttcg gatagttcga 1380
gatctgtgat ccatggttag taccctaggc agtggggtta gatccgtgct gttatggttc 1440
gtagatggat tctgattgct cagtaactgg gaatcctggg atggttctag ctggttcgca 1500
gataagatcg atttcatgat atgctatatc ttgtttggtt gccgtggttc cgttaaatct 1560
gtctgttatg atcttagtct ttgataaggt tcggtcgtgc tagctacgtc ctgtgcagca 1620
cttaattgtc aggtcataat ttttagcatg cctttttttt attggtttgg ttttgtctga 1680
ctgggctgta gatagtttca atctttgtct gactgggctg tagatagttt caatcttcct 1740
gtctgtttat tttattaaat ttggatctgt atgtgtgtca tatatcttca tcttttagat 1800
atatcgatag gtatatatgt tgctgtcgtt ttttactgtt cctttatgag atatattcat 1860
gcttagatac atgaaacaac gtgctgttac agtttaatag ttcttgttta tctaataaac 1920
aaataaggat aggtgctgca gttagtttta ctggtacttt ttttgacatg aacctacggc 1980
ttaataatta gtcttcatca aataaaaagc atatttttta attatttcga tatacttgaa 2040
tgatgtcata tgcagcatct gtgtgaattt ttggccctgt cttcatatga tgtttatttg 2100
cttgggactg tttctttggc tgataactca ccctgttgtt tggtgatcct tctgcaggtg 2160
<210> 56
<211> 1024
<212> DNA
<213> Bouteloua gracilis
<400> 56
gagaaatcgg ggaaaaatca tgttttggtt gtttttattt ctaacctcca caataacttt 60
ggtttaccat tttttgtttg attttagttt tagagaagcg tttataacag gacctaaaat 120
ctttttttga gtacacagta caacgcagac gctcatacac gcacgcacaa tgtcctctat 180
gaacacacgt aaggaaaccc tacaccttga gcaccttcga aggactgagc cggcaaatct 240
agagattctc gaagtcacta ttggcacctc gttatcaacg agaacgtcgc ttaccactta 300
aagcataaca ccgagaaatc ccgtaacaaa tccagtaaaa tacgagcacc cgtaccaagt 360
tgaatatttg aacccgagtg ggtagattcc accgcaaagg acctaaccag atcatttcgc 420
aaacaggaac taaaatcggt agagagccca gacaaaaacc ttttctaaga gcaactccag 480
tgaaagcccc tactttaggt ataaaatgca acactagtgg agcttctaaa taaacttcta 540
tttttcatgc cctcctaaaa tttactccta aaaccctagc tataggagcc tcctatccat 600
cctctatttt attccactag aattgattat aaatttagcc tcttaaattt tataagttgg 660
gagtcgaggg taactagagt tgctctaaac ggaccttatc ttcaagtgac ctcagtgagc 720
ccgtttaacg gcgtcgacaa gtctaatcta acggacacca accagagaac caccgccagc 780
gccgagccaa gcgacgttga catcttggcg cggcacggca tctccctggc gtctggtccc 840
ctcccgagac ttccgctcca cctcccaccg gtggcggttt ccgagtccgt tccgcctcct 900
ctcacacggc acgaaacctt gacggcaccg gcagcacggg ggattccgtt cccacggctc 960
cttccctttc ccttcctcgc ccgctgctat aaatagccag ccccatcccc agcttcttcc 1020
ccaa 1024
<210> 57
<211> 1045
<212> DNA
<213> Bouteloua gracilis
<400> 57
gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 60
ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 120
ctgctagttc tgttcctgtt tttttttttc catggctgcg aggcacaata gatctgatgg 180
cgttatgatg gttaacttgt catactcttg cgatctatgg tccctttagg agtttaggac 240
atcgatttaa tttcggatag ttcgagatct gtgatccatg gttagtaccc taggcagtgg 300
ggttagatcc gtgctgttat ggttcgtaga tggattctga ttgctcagta actgggaatc 360
ctgggatggt tctagctggt tcgcagataa gatcgatttc atgatatgct atatcttgtt 420
tggttgccgt ggttccgtta aatctgtctg ttatgatctt agtctttgat aaggttcggt 480
cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt 540
tttttattgg tttggttttg tctgactggg ctgtagatag tttcaatctt tgtctgactg 600
ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg 660
tgtcatatat cttcatcttt tagatatatc gataggtata tatgttgctg tcgtttttta 720
ctgttccttt atgagatata ttcatgctta gatacatgaa acaacgtgct gttacagttt 780
aatagttctt gtttatctaa taaacaaata aggataggtg ctgcagttag ttttactggt 840
actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt 900
ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc 960
cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg 1020
ttgtttggtg atccttctgc aggtg 1045
<210> 58
<211> 2160
<212> DNA
<213> Bouteloua gracilis
<400> 58
gagaaatcgg ggaaaaatca tgttttggtt gtttttattt ctaacctcca caataacttt 60
ggtttaccat tttttgtttg attttagttt tagagaagcg tttataacag gacctaaaat 120
ctttttttga gtacacagta caacgcagac gctcatacac gcacgcacaa tgtcctctat 180
gaacacacgt aaggaaaccc tacaccttga gcaccttcga aggactgagc cggcaaatct 240
agagattctc gaagtcacta ttggcacctc gttatcaacg agaacgtcgc ttaccactta 300
aagcataaca ccgagaaatc ccgtaacaaa tccagtaaaa tacgagcacc cgtaccaagt 360
tgaatatttg aacccgagtg ggtagattcc accgcaaagg acctaaccag atcatttcgc 420
aaacaggaac taaaatcggt agagagccca gacaaaaacc ttttctaaga gcaactccag 480
tgaaagcccc tactttaggt ataaaatgca acactagtgg agcttctaaa taaacttcta 540
tttttcatgc cctcctaaaa tttactccta aaaccctagc tataggagcc tcctatccat 600
cctctatttt attccactag aattgattat aaatttagcc tcttaaattt tataagttgg 660
gagtcgaggg taactagagt tgctctaaac ggaccttatc ttcaagtgac ctcagtgagc 720
ccgtttaacg gcgtcgacaa gtctaatcta acggacacca accagagaac caccgccagc 780
gccgagccaa gcgacgttga catcttggcg cggcacggca tctccctggc gtctggtccc 840
ctcccgagac ttccgctcca cctcccaccg gtggcggttt ccgagtccgt tccgcctcct 900
ctcacacggc acgaaacctt gacggcaccg gcagcacggg ggattccgtt cccacggctc 960
cttccctttc ccttcctcgc ccgctgctat aaatagccag ccccatcccc agcttcttcc 1020
ccaacctcat cttctcgtgt tgttcggccc aacccgatcg atccccaatt ccctcgtcgt 1080
ctctcgtcgc gagcctcgtc gatccccgct tcaaggtaca gcgatcgatc gatcatcctc 1140
gctctctcta ccttctctct cttagggcgt gctggttctg ttcctgtttt tccatggctg 1200
cgaggtacaa tagattggcg attcatggtt agggcctgct agttctgttc ctgttttttt 1260
tttttccatg gctgcgaggc acaatagatc tgatggcgtt atgatggtta acttgtcata 1320
ctcttgcgat ctatggtccc tttaggagtt taggacatcg atttaatttc ggatagttcg 1380
agatctgtga tccatggtta gtaccctagg cagtggggtt agatccgtgc tgttatggtt 1440
cgtagatgga ttctgattgc tcagtaactg ggaatcctgg gatggttcta gctggttcgc 1500
agataagatc gatttcatga tatgctatat cttgtttggt tgccgtggtt ccgttaaatc 1560
tgtctgttat gatcttagtc ttgataaggt tcggtcgtgc tagctacgtc ctgtgcagca 1620
cttaattgtc aggtcataat ttttagcatg cctttttttt attggtttgg ttttgtctga 1680
ctgggctgta gatagtttca atctttgtct gactgggctg tagatagttt caatcttcct 1740
gtctgtttat tttattaaat ttggatctgt atgtgtgtca tatatcttca tcttttagat 1800
atatcgatag gtatatatgt tgctgtcgtt ttttactgtt cctttatgag atatattcat 1860
gcttagatac atgaaacaac gtgctgttac agtttaatag ttcttgttta tctaataaac 1920
aaataaggat aggtgctgca gttagtttta ctggtacttt ttttgacatg aacctacggc 1980
ttaataatta gtcttcatca aataaaaagc atatttttta attatttcga tatacttgaa 2040
tgatgtcata tgcagcatct gtgtgaattt ttggccctgt cttcatatga tgtttatttg 2100
cttgggactg tttctttggc tgataactca ccctgttgtt tggtgatcct tctgcaggtg 2160
<210> 59
<211> 1045
<212> DNA
<213> Bouteloua gracilis
<400> 59
gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 60
ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 120
ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg 180
gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga 240
catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg 300
gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat 360
cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt 420
ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtcttgat aaggttcggt 480
cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt 540
tttttattgg tttggttttg tctgactggg ctgtagatag tttcaatctt tgtctgactg 600
ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg 660
tgtcatatat cttcatcttt tagatatatc gataggtata tatgttgctg tcgtttttta 720
ctgttccttt atgagatata ttcatgctta gatacatgaa acaacgtgct gttacagttt 780
aatagttctt gtttatctaa taaacaaata aggataggtg ctgcagttag ttttactggt 840
actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt 900
ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc 960
cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg 1020
ttgtttggtg atccttctgc aggtg 1045
<210> 60
<211> 1885
<212> DNA
<213> Bouteloua gracilis
<400> 60
caacgagaac gtcgcttacc acttaaagca taacaccgag aaatcccgta acaaatccag 60
taaaatacga gcacccgtac caagttgaat atttgaaccc gagtgggtag attccaccgc 120
aaaggaccta accagatcat ttcgcaaaca ggaactaaaa tcggtagaga gcccagacaa 180
aaaccttttc taagagcaac tccagtgaaa gcccctactt taggtataaa atgcaacact 240
agtggagctt ctaaataaac ttctattttt catgccctcc taaaatttac tcctaaaacc 300
ctagctatag gagcctccta tccatcctct attttattcc actagaattg attataaatt 360
tagcctctta aattttataa gttgggagtc gagggtaact agagttgctc taaacggacc 420
ttatcttcaa gtgacctcag tgagcccgtt taacggcgtc gacaagtcta atctaacgga 480
caccaaccag agaaccaccg ccagcgccga gccaagcgac gttgacatct tggcgcggca 540
cggcatctcc ctggcgtctg gtcccctccc gagacttccg ctccacctcc caccggtggc 600
ggtttccgag tccgttccgc ctcctctcac acggcacgaa accttgacgg caccggcagc 660
acgggggatt ccgttcccac ggctccttcc ctttcccttc ctcgcccgct gctataaata 720
gccagcccca tccccagctt cttccccaac ctcatcttct cgtgttgttc ggcccaaccc 780
gatcgatccc caattccctc gtcgtctctc gtcgcgagcc tcgtcgatcc ccgcttcaag 840
gtacagcgat cgatcgatca tcctcgctct ctctaccttc tctctcttag ggcgtgctgg 900
ttctgttcct gtttttccat ggctgcgagg tacaatagat tggcgattca tggttagggc 960
ctgctagttc tgttcctgtt tttttttttt ccatggctgc gaggcacaat agatctgatg 1020
gcgttatgat ggttaacttg tcatactctt gcgatctatg gtccctttag gagtttagga 1080
catcgattta atttcggata gttcgagatc tgtgatccat ggttagtacc ctaggcagtg 1140
gggttagatc cgtgctgtta tggttcgtag atggattctg attgctcagt aactgggaat 1200
cctgggatgg ttctagctgg ttcgcagata agatcgattt catgatatgc tatatcttgt 1260
ttggttgccg tggttccgtt aaatctgtct gttatgatct tagtcttgat aaggttcggt 1320
cgtgctagct acgtcctgtg cagcacttaa ttgtcaggtc ataattttta gcatgccttt 1380
tttttattgg tttggttttg tctgactggg ctgtagatag tttcaatctt tgtctgactg 1440
ggctgtagat agtttcaatc ttcctgtctg tttattttat taaatttgga tctgtatgtg 1500
tgtcatatat cttcatcttt tagatatatc gataggtata tatgttgctg tcgtttttta 1560
ctgttccttt atgagatata ttcatgctta gatacatgaa acaacgtgct gttacagttt 1620
aatagttctt gtttatctaa taaacaaata aggataggtg ctgcagttag ttttactggt 1680
actttttttg acatgaacct acggcttaat aattagtctt catcaaataa aaagcatatt 1740
ttttaattat ttcgatatac ttgaatgatg tcatatgcag catctgtgtg aatttttggc 1800
cctgtcttca tatgatgttt atttgcttgg gactgtttct ttggctgata actcaccctg 1860
ttgtttggtg atccttctgc aggtg 1885
<210> 61
<211> 749
<212> DNA
<213> Bouteloua gracilis
<400> 61
caacgagaac gtcgcttacc acttaaagca taacaccgag aaatcccgta acaaatccag 60
taaaatacga gcacccgtac caagttgaat atttgaaccc gagtgggtag attccaccgc 120
aaaggaccta accagatcat ttcgcaaaca ggaactaaaa tcggtagaga gcccagacaa 180
aaaccttttc taagagcaac tccagtgaaa gcccctactt taggtataaa atgcaacact 240
agtggagctt ctaaataaac ttctattttt catgccctcc taaaatttac tcctaaaacc 300
ctagctatag gagcctccta tccatcctct attttattcc actagaattg attataaatt 360
tagcctctta aattttataa gttgggagtc gagggtaact agagttgctc taaacggacc 420
ttatcttcaa gtgacctcag tgagcccgtt taacggcgtc gacaagtcta atctaacgga 480
caccaaccag agaaccaccg ccagcgccga gccaagcgac gttgacatct tggcgcggca 540
cggcatctcc ctggcgtctg gtcccctccc gagacttccg ctccacctcc caccggtggc 600
ggtttccgag tccgttccgc ctcctctcac acggcacgaa accttgacgg caccggcagc 660
acgggggatt ccgttcccac ggctccttcc ctttcccttc ctcgcccgct gctataaata 720
gccagcccca tccccagctt cttccccaa 749
<210> 62
<211> 6813
<212> DNA
<213> Miscanthus sinesis
<400> 62
agcagactcg cattatcgat gggggaaatg aaattcagcg tttgacgtgg atgcaacaac 60
tgcactgcac aggatatctt agccgttgtg tcgaagtttg ctttgctaac gttttgagaa 120
aaccagcttt gaccaacacg agacgagcgc cttacgtttg gcacaatgta atgtagcccg 180
gcacggcaag ttagactagt atattgtgtt agccggcctc tttacgtttg gcacagttta 240
attgaatccg gcatggcaag ttagactgga gtgtgagccg gtcattgcaa agttattatg 300
acatatatat aagagcacaa gtgtataata agataatgta agcaaggcag caagctatat 360
gaattgtcac gttatattta tgttgagatg ttgagatgaa gaagagaaaa taaacagcct 420
ataaattcat agcgagtgat agacgggcac aaggcctcct atttcttaaa ccgaattttg 480
taagaacaaa aaaaaggact tataggagaa tgggatagac catatatcaa cgggaaaggt 540
acacgttgct cgagtgtttt aggcgttctg ctcactcgat cctgtagctg tccgatctgc 600
ggcgtcaaca cggcgcgcaa caagcggtgg cgggcccctc ggtagccgcg gtcggaccgg 660
acgatggcct atggcgaccc gcggcctggg cgtggcctgt gcgtgcatgc gccataggtc 720
ccggtgcatg gtgcaggcgg caggtgcatg tgcatggagt aggctttggt gctggtgcag 780
gctttggtca ggtgcaggag gggtaggttg cgcaggtgag aggtgaggtg catgctgacc 840
cgtcacatca ccttactcct agcccctaag tcttgcatgt atgcagattt attcttttag 900
cagcgacaga ttcagcagcg agagaccggc taccgtagca ttttcatttt tatttgataa 960
ttagtattta attatggact aattaggttc aaaatattcg tctcgcgatt tccaaccaaa 1020
ctgtgcaatt agtttttttc gtctacattt aatgctctat acacgtatca caagattcaa 1080
cgtgatggct actgtagcac tttttgaaaa aactttttgc aactaaacaa ggcctgaggt 1140
atcgtttaaa tttaggtaca aaaaatataa gggtgtcaca tcgaatgtta cacgagatat 1200
catatgtgag tgttcggata gtaataataa aataaattac acaagtcctt agtaatccac 1260
gagacgaatt tattgagtct aattaatcag tcattagcac atggtgcatt catgcatctg 1320
catattattt tgtgttgctt ggttgaaagt tggatttcaa attgagttga atttgcattt 1380
tgaaattgct ttggaaaaat tagaaaaaaa gaaaaaaaat gaatttccct ccctcctttc 1440
tcatttccct gctttcggcc cctctgtgta gaactattcg agttctcagg tcgagtgctc 1500
gaatcatcta gcttctcttt tttgaggaga gccagagagc cagattcaga atagccagcc 1560
tcctttttag gagagagctc atcccctttt atagttgaag gcagcgacga agccagcggg 1620
gggctacccg tgctccagcc tccctacggc catgatttac atggaacccg ggcttagctc 1680
gggctaccgc catgaggagg aagaagaaga taaggagggg ctagaggaag aagaagagga 1740
agctagccct ggcttcgtcg attcctggct tcgtcgctgg ttgaagggga tgggctctta 1800
caagtcagag aaagagagag aatgtatacg tgtgctatct agtcttgttg cccacgctgt 1860
caggtacgag acggttgtcg gcgcccacaa tactgtttat gtccagatgc atgtggcagg 1920
ctctaccgtg ttcgcctgtt atggcaaatg tcggcgcata caatactatt tgggttctga 1980
cacgcctgaa aggttgcata gtgcctatct ggcatggcct ggtggcaccg tccggcatgt 2040
gcgcaggata tgccagggta cggtccttgg tattacggtt tgacttgagc gccttacctt 2100
atctgctccg cctgatcccc gggctcttac cgagcgggcg tccccggtcg gtcgttccca 2160
gtcggccccg actgtgtcgg tcggggaaga gctgcaagca gaggtccggc gtatccccga 2220
tcgaaaaagg aagtcggagt cagactatgt ctccacctta gccaggcctt ccggtcgggg 2280
atcggatcat tctcccggcc tgtcattagg tatctgggtc ggcccgagag gtgtgcgttg 2340
tcgctacgct gtctgctggg ccgagtttct gttgggaagc gggtccattg gggaccccgg 2400
gtttatgaac ccgacacgtg gtcactatgc tgcatactcc ctatacagcc gctgaccagt 2460
acgctggttc accgcgtcgc ccgcgcggga cggaatggga tgtcacgacc cgctgaacgc 2520
cggggcatgg catcagcggc gaacaggcac ccggcgtgga gctgtccgtg tcaccatcta 2580
cagtgttgac gggacccgca taaaaggaga aaaaaggccc gacggtcctg gaagccttcc 2640
tctccttagc tcttctccct ctttctctct gtgtaacctg ctcttcccct tcgtctataa 2700
aaagggaagt aggacgtccc aggaagagaa gggcggttca ccactctaca tggctataga 2760
cataaaaaca cacgccttgg gagcacactc acatcagaga cttgggacct atccctctct 2820
cgctcgtttg taacccctac tacaaacttt tagtgctagt aacacgagca gcagcgacga 2880
actagacgta aggactttct gcccgaacca gtataaacat cgtgtcatct aagcacacca 2940
tacgagccag acgcgcaata ctagaaattt actagtcggt aactcgaaac accgacatct 3000
agctaatctt tttgttttat ttggtttccc tttgaaatct tctaatttag ctttcataga 3060
aataatctag gtatttttta ttttatatgt tctatctgtt tgcattaatt ttgatcattt 3120
gatctgaatg ctgtggtcac gagaatcgag tgtttcatgg ccttaaaaca ctcgattatg 3180
ccatctgacc cgttttcaac cattctagtg tttctgagct atatcaatgg tgcagcatgt 3240
tagtatacat atctaactat tactccgtat atgagtgagt tgttaaattt attccaggtg 3300
aaatggcatt aacgatagcc aataggcggc taaattaata gccatactct aacagctcta 3360
aaaaacatat attcatcgag gcacctttat gcaaccacat agtcaacttc aacgtcgctt 3420
gcgtgcgttc tcaagttttc tttcttgcaa attacatttt ttttaaaaaa aagtataatt 3480
tgtatcgtgc gattttttct ctctaggtgt gcgtgactgt gggagtaaca attttgaatc 3540
tcaagaagga aataaaagaa taatactgct gcctactttg aggatttcag tatttttctc 3600
taaaatgttt tggtgtgata tctaaaccgt ctttaaagcc aattgctcaa gattcattca 3660
acaattgaaa cgtctcacat gactaaatga tataaggttg ctaaggtctt tcttgataag 3720
cttttttatg aatttcatct aaattttcga gtgaaactat taaatactaa ggttgctaag 3780
tgtcattctc gctcgagaag tctaacgctt taaactttaa ccaaatatat acaagaaaat 3840
attaatattt atagtacata attagtatca ttagatagat cgttgaatct attttcataa 3900
caaacttatt tgaagaaaca aatgttgttc atatatttct atatacgaat accatagcga 3960
cacttatttt agaatgtagg gagtactccc tttgtgccgc tttgagtgtc gctttggcag 4020
ctagtaccta tgtccacctt cacagcttgt gcctagtacc tagactcttt ctctgtccac 4080
attcatttaa tctctgttgt accttgttcg gagataaaac gactctgata aagggacgag 4140
gaagtagtat gttagaggag tgaagtctac tccctttgcc gcaaaaaggt aatcctaagt 4200
gtgaattgta ttcttttttg accaaaggaa tatacaacaa gaatgatgtc atcatcatgc 4260
ttcgatcctt ttttttggta aagcttgagc ttctgtaaaa atagagaaat catgggaaaa 4320
atcacgtttt ggtggttttg atttctagcc tccacaataa ctttggtttt actatttttt 4380
gtttgatttt agtttcagaa gtccactttt gtacgtgctc gtagagccta aacaaaaggc 4440
tttccaaaac gaccttatct tcgagtgttg taaaaaaaat gagcccgttt aacggcgtcg 4500
acaagtctaa cggacaccaa ccagcgaacc accagcgccg agccaagcga agcagactgc 4560
agacggcacg gccgagacgt tgacaccttg gcgcggcaac ggcatctctc tggccccctc 4620
tcgagagttc cgctccacct ccgcatccac ctccacctcc acctccaccg gtggcggttt 4680
ccaagtccgt cccgttccgc cacctgctcc tctcacacgg cacgaaaccg tcacggcacc 4740
ggcagcacag cacgggggat tcctttccca ccgctccgtc cctttctctt cctcgcccgc 4800
ccgttataaa tagccagccc catccctcgt ctctcgtgtt gttcggagcg cacacacaac 4860
ccgatcccca atcaatcgat ccccgcttca aggtacggcg atcctcctcc ctctctctct 4920
accttctctt ctctacacta gatcggcggt ccatggttag ggcctgctag ttccgttcct 4980
gtttttccat ggctgcgagg tacaatagat ctgatggcgt tatgatggtt aacttgtcat 5040
gcttttgcga tttatagtcc ctttagatag ttcgagatcg gtgatccatg gttagtaccc 5100
taggctgtgg agtcgggtta gatccgcgct gttagggttc gtatatggag gcgagctgtt 5160
ctgattgtta acttgctggg aatcctggga tggttctagc tgttccgcag atgagatcga 5220
tttcatgatc tgctgtatct atccgtggta tgatgttagc ctttgatatg gttcgatcgt 5280
gctagctacg tcctgtgcac ttaattgtca ggtcataatt tttactatac tttttttttg 5340
gtttggtttg gtttcgtctg atttggctgt cgttctagat cagagtagaa actgtttcaa 5400
actacctgtt ggatttatta aggtagcgtt tggttcctgg tatcgaatca tacacgcacc 5460
agtgcatctt ggatagccag ctggggccca cctgtccaac cgtttggttg ccggatcgaa 5520
cgagtccatt caagaccgaa ccatgcagag caatcgaata ttctcttgtg acgctgtatc 5580
atccagttcg gcaaaaaaca ccgaatgccg ccatacagga caccgtactg agcgtctgca 5640
actctgcatc ccgctcactg ctcacatctc cgcttgccgc ctcacccatc cgactcagac 5700
cagagccaca cggattactg ctgctggtgt gtgtattaac aaaagatcca tttgaccgga 5760
gcacatgcag cttggatgga aaaaatttat tatattcgtc agtgctgcat atgtactcat 5820
acttgcatga tggttttatt tattcgacct catcagtcct ggcactatgg aaagtcattg 5880
tagtatagat tttttaatat aatataaatc attggtgact tatcttgctt aattttattt 5940
tcttattatg aaatatcgtt gcattcataa tagcaaattt gtgcaaatat atagaatcta 6000
cgtgaaattc ttggttggac caatacaaca aacccctcaa acattctctt gtactgaacc 6060
ataccattcc gtacaaccat ccaaacaaaa atcatgtatc atcatgtaca tgtaaccaaa 6120
caattaacac gcaccatcct attcagactt gtctcatcca taatctatcc atccaggatg 6180
atccatccca ttcatctata tacacccaat caaacgctac ctaaaatttg gatctgtatg 6240
tgtcacatat atcttaataa gatggatgga aatatctctt tatcttttag atatggatag 6300
gtatatatgt tgctgtgggt ttgttagtta tatatatacg tgcttacata cgtgaagaaa 6360
cctgctgcta cagtttaata attcttgttc atctcaacaa ataacgatag gcgtatatgt 6420
tgctgtgttt tttactggta ctttgttaga tatatacatg cttacataca tgaagaacac 6480
atgctacagt tcaaaaattc ttgttcatct cataaacaaa aaggaggtgt atatgttgct 6540
gtgggtttta ctggtacttt attagatata tacatgctta catagatgaa gcaacatgct 6600
gctatggtgt ttaataatta ttgtttatct aataaacaaa catgcttttt aattatcttg 6660
atatgtttgg atgatggcat atgcagcagc tatgtgtgga ttttaaatac ccagcatcat 6720
gagcatgcat gaccctgcct tagtatgcag ttatttgctt gagactgttt cttttgttga 6780
tactcatcct ttagttcggt cactcttctg cag 6813
<210> 63
<211> 5359
<212> DNA
<213> Miscanthus sinesis
<400> 63
agcagactcg cattatcgat gggggaaatg aaattcagcg tttgacgtgg atgcaacaac 60
tgcactgcac aggatatctt agccgttgtg tcgaagtttg ctttgctaac gttttgagaa 120
aaccagcttt gaccaacacg agacgagcgc cttacgtttg gcacaatgta atgtagcccg 180
gcacggcaag ttagactagt atattgtgtt agccggcctc tttacgtttg gcacagttta 240
attgaatccg gcatggcaag ttagactgga gtgtgagccg gtcattgcaa agttattatg 300
acatatatat aagagcacaa gtgtataata agataatgta agcaaggcag caagctatat 360
gaattgtcac gttatattta tgttgagatg ttgagatgaa gaagagaaaa taaacagcct 420
ataaattcat agcgagtgat agacgggcac aaggcctcct atttcttaaa ccgaattttg 480
taagaacaaa aaaaaggact tataggagaa tgggatagac catatatcaa cgggaaaggt 540
acacgttgct cgagtgtttt aggcgttctg ctcactcgat cctgtagctg tccgatctgc 600
ggcgtcaaca cggcgcgcaa caagcggtgg cgggcccctc ggtagccgcg gtcggaccgg 660
acgatggcct atggcgaccc gcggcctggg cgtggcctgt gcgtgcatgc gccataggtc 720
ccggtgcatg gtgcaggcgg caggtgcatg tgcatggagt aggctttggt gctggtgcag 780
gctttggtca ggtgcaggag gggtaggttg cgcaggtgag aggtgaggtg catgctgacc 840
cgtcacatca ccttactcct agcccctaag tcttgcatgt atgcagattt attcttttag 900
cagcgacaga ttcagcagcg agagaccggc taccgtagca ttttcatttt tatttgataa 960
ttagtattta attatggact aattaggttc aaaatattcg tctcgcgatt tccaaccaaa 1020
ctgtgcaatt agtttttttc gtctacattt aatgctctat acacgtatca caagattcaa 1080
cgtgatggct actgtagcac tttttgaaaa aactttttgc aactaaacaa ggcctgaggt 1140
atcgtttaaa tttaggtaca aaaaatataa gggtgtcaca tcgaatgtta cacgagatat 1200
catatgtgag tgttcggata gtaataataa aataaattac acaagtcctt agtaatccac 1260
gagacgaatt tattgagtct aattaatcag tcattagcac atggtgcatt catgcatctg 1320
catattattt tgtgttgctt ggttgaaagt tggatttcaa attgagttga atttgcattt 1380
tgaaattgct ttggaaaaat tagaaaaaaa gaaaaaaaat gaatttccct ccctcctttc 1440
tcatttccct gctttcggcc cctctgtgta gaactattcg agttctcagg tcgagtgctc 1500
gaatcatcta gcttctcttt tttgaggaga gccagagagc cagattcaga atagccagcc 1560
tcctttttag gagagagctc atcccctttt atagttgaag gcagcgacga agccagcggg 1620
gggctacccg tgctccagcc tccctacggc catgatttac atggaacccg ggcttagctc 1680
gggctaccgc catgaggagg aagaagaaga taaggagggg ctagaggaag aagaagagga 1740
agctagccct ggcttcgtcg attcctggct tcgtcgctgg ttgaagggga tgggctctta 1800
caagtcagag aaagagagag aatgtatacg tgtgctatct agtcttgttg cccacgctgt 1860
caggtacgag acggttgtcg gcgcccacaa tactgtttat gtccagatgc atgtggcagg 1920
ctctaccgtg ttcgcctgtt atggcaaatg tcggcgcata caatactatt tgggttctga 1980
cacgcctgaa aggttgcata gtgcctatct ggcatggcct ggtggcaccg tccggcatgt 2040
gcgcaggata tgccagggta cggtccttgg tattacggtt tgacttgagc gccttacctt 2100
atctgctccg cctgatcccc gggctcttac cgagcgggcg tccccggtcg gtcgttccca 2160
gtcggccccg actgtgtcgg tcggggaaga gctgcaagca gaggtccggc gtatccccga 2220
tcgaaaaagg aagtcggagt cagactatgt ctccacctta gccaggcctt ccggtcgggg 2280
atcggatcat tctcccggcc tgtcattagg tatctgggtc ggcccgagag gtgtgcgttg 2340
tcgctacgct gtctgctggg ccgagtttct gttgggaagc gggtccattg gggaccccgg 2400
gtttatgaac ccgacacgtg gtcactatgc tgcatactcc ctatacagcc gctgaccagt 2460
acgctggttc accgcgtcgc ccgcgcggga cggaatggga tgtcacgacc cgctgaacgc 2520
cggggcatgg catcagcggc gaacaggcac ccggcgtgga gctgtccgtg tcaccatcta 2580
cagtgttgac gggacccgca taaaaggaga aaaaaggccc gacggtcctg gaagccttcc 2640
tctccttagc tcttctccct ctttctctct gtgtaacctg ctcttcccct tcgtctataa 2700
aaagggaagt aggacgtccc aggaagagaa gggcggttca ccactctaca tggctataga 2760
cataaaaaca cacgccttgg gagcacactc acatcagaga cttgggacct atccctctct 2820
cgctcgtttg taacccctac tacaaacttt tagtgctagt aacacgagca gcagcgacga 2880
actagacgta aggactttct gcccgaacca gtataaacat cgtgtcatct aagcacacca 2940
tacgagccag acgcgcaata ctagaaattt actagtcggt aactcgaaac accgacatct 3000
agctaatctt tttgttttat ttggtttccc tttgaaatct tctaatttag ctttcataga 3060
aataatctag gtatttttta ttttatatgt tctatctgtt tgcattaatt ttgatcattt 3120
gatctgaatg ctgtggtcac gagaatcgag tgtttcatgg ccttaaaaca ctcgattatg 3180
ccatctgacc cgttttcaac cattctagtg tttctgagct atatcaatgg tgcagcatgt 3240
tagtatacat atctaactat tactccgtat atgagtgagt tgttaaattt attccaggtg 3300
aaatggcatt aacgatagcc aataggcggc taaattaata gccatactct aacagctcta 3360
aaaaacatat attcatcgag gcacctttat gcaaccacat agtcaacttc aacgtcgctt 3420
gcgtgcgttc tcaagttttc tttcttgcaa attacatttt ttttaaaaaa aagtataatt 3480
tgtatcgtgc gattttttct ctctaggtgt gcgtgactgt gggagtaaca attttgaatc 3540
tcaagaagga aataaaagaa taatactgct gcctactttg aggatttcag tatttttctc 3600
taaaatgttt tggtgtgata tctaaaccgt ctttaaagcc aattgctcaa gattcattca 3660
acaattgaaa cgtctcacat gactaaatga tataaggttg ctaaggtctt tcttgataag 3720
cttttttatg aatttcatct aaattttcga gtgaaactat taaatactaa ggttgctaag 3780
tgtcattctc gctcgagaag tctaacgctt taaactttaa ccaaatatat acaagaaaat 3840
attaatattt atagtacata attagtatca ttagatagat cgttgaatct attttcataa 3900
caaacttatt tgaagaaaca aatgttgttc atatatttct atatacgaat accatagcga 3960
cacttatttt agaatgtagg gagtactccc tttgtgccgc tttgagtgtc gctttggcag 4020
ctagtaccta tgtccacctt cacagcttgt gcctagtacc tagactcttt ctctgtccac 4080
attcatttaa tctctgttgt accttgttcg gagataaaac gactctgata aagggacgag 4140
gaagtagtat gttagaggag tgaagtctac tccctttgcc gcaaaaaggt aatcctaagt 4200
gtgaattgta ttcttttttg accaaaggaa tatacaacaa gaatgatgtc atcatcatgc 4260
ttcgatcctt ttttttggta aagcttgagc ttctgtaaaa atagagaaat catgggaaaa 4320
atcacgtttt ggtggttttg atttctagcc tccacaataa ctttggtttt actatttttt 4380
gtttgatttt agtttcagaa gtccactttt gtacgtgctc gtagagccta aacaaaaggc 4440
tttccaaaac gaccttatct tcgagtgttg taaaaaaaat gagcccgttt aacggcgtcg 4500
acaagtctaa cggacaccaa ccagcgaacc accagcgccg agccaagcga agcagactgc 4560
agacggcacg gccgagacgt tgacaccttg gcgcggcaac ggcatctctc tggccccctc 4620
tcgagagttc cgctccacct ccgcatccac ctccacctcc acctccaccg gtggcggttt 4680
ccaagtccgt cccgttccgc cacctgctcc tctcacacgg cacgaaaccg tcacggcacc 4740
ggcagcacag cacgggggat tcctttccca ccgctccgtc cctttctctt cctcgcccgc 4800
ccgttataaa tagccagccc catccctcgt ctctcgtgtt gttcggagcg cacacacaac 4860
ccgatcccca atcaatcgat ccccgcttca aggtacggcg atcctcctcc ctctctctct 4920
accttctctt ctctacacta gatcggcggt ccatggttag ggcctgctag ttccgttcct 4980
gtttttccat ggctgcgagg tacaatagat ctgatggcgt tatgatggtt aacttgtcat 5040
gcttttgcga tttatagtcc ctttagatag ttcgagatcg gtgatccatg gttagtaccc 5100
taggctgtgg agtcgggtta gatccgcgct gttagggttc gtatatggag gcgagctgtt 5160
ctgattgtta acttgctggg aatcctggga tggttctagc tgttccgcag atgagatcga 5220
tttcatgatc tgctgtatct atccgtggta tgatgttagc ctttgatatg gttcgatcgt 5280
gctagctacg tcctgtgcac ttaattgtca ggtcataatt tttactatac tttttttttg 5340
gtttggtttg gtttcgtct 5359
<210> 64
<211> 63
<212> DNA
<213> Miscanthus sinesis
<400> 64
gatttggctg tcgttctaga tcagagtaga aactgtttca aactacctgt tggatttatt 60
aag 63
<210> 65
<211> 1391
<212> DNA
<213> Miscanthus sinesis
<400> 65
gtagcgtttg gttcctggta tcgaatcata cacgcaccag tgcatcttgg atagccagct 60
ggggcccacc tgtccaaccg tttggttgcc ggatcgaacg agtccattca agaccgaacc 120
atgcagagca atcgaatatt ctcttgtgac gctgtatcat ccagttcggc aaaaaacacc 180
gaatgccgcc atacaggaca ccgtactgag cgtctgcaac tctgcatccc gctcactgct 240
cacatctccg cttgccgcct cacccatccg actcagacca gagccacacg gattactgct 300
gctggtgtgt gtattaacaa aagatccatt tgaccggagc acatgcagct tggatggaaa 360
aaatttatta tattcgtcag tgctgcatat gtactcatac ttgcatgatg gttttattta 420
ttcgacctca tcagtcctgg cactatggaa agtcattgta gtatagattt tttaatataa 480
tataaatcat tggtgactta tcttgcttaa ttttattttc ttattatgaa atatcgttgc 540
attcataata gcaaatttgt gcaaatatat agaatctacg tgaaattctt ggttggacca 600
atacaacaaa cccctcaaac attctcttgt actgaaccat accattccgt acaaccatcc 660
aaacaaaaat catgtatcat catgtacatg taaccaaaca attaacacgc accatcctat 720
tcagacttgt ctcatccata atctatccat ccaggatgat ccatcccatt catctatata 780
cacccaatca aacgctacct aaaatttgga tctgtatgtg tcacatatat cttaataaga 840
tggatggaaa tatctcttta tcttttagat atggataggt atatatgttg ctgtgggttt 900
gttagttata tatatacgtg cttacatacg tgaagaaacc tgctgctaca gtttaataat 960
tcttgttcat ctcaacaaat aacgataggc gtatatgttg ctgtgttttt tactggtact 1020
ttgttagata tatacatgct tacatacatg aagaacacat gctacagttc aaaaattctt 1080
gttcatctca taaacaaaaa ggaggtgtat atgttgctgt gggttttact ggtactttat 1140
tagatatata catgcttaca tagatgaagc aacatgctgc tatggtgttt aataattatt 1200
gtttatctaa taaacaaaca tgctttttaa ttatcttgat atgtttggat gatggcatat 1260
gcagcagcta tgtgtggatt ttaaataccc agcatcatga gcatgcatga ccctgcctta 1320
gtatgcagtt atttgcttga gactgtttct tttgttgata ctcatccttt agttcggtca 1380
ctcttctgca g 1391
<210> 66
<211> 4402
<212> DNA
<213> Miscanthus sinesis
<400> 66
cacgtggtca ctatgctgca tactccctat acagccgctg accagtacgc tggttcaccg 60
cgtcgcccgc gcgggacgga atgggatgtc acgacccgct gaacgccggg gcatggcatc 120
agcggcgaac aggcacccgg cgtggagctg tccgtgtcac catctacagt gttgacggga 180
cccgcataaa aggagaaaaa aggcccgacg gtcctggaag ccttcctctc cttagctctt 240
ctccctcttt ctctctgtgt aacctgctct tccccttcgt ctataaaaag ggaagtagga 300
cgtcccagga agagaagggc ggttcaccac tctacatggc tatagacata aaaacacacg 360
ccttgggagc acactcacat cagagacttg ggacctatcc ctctctcgct cgtttgtaac 420
ccctactaca aacttttagt gctagtaaca cgagcagcag cgacgaacta gacgtaagga 480
ctttctgccc gaaccagtat aaacatcgtg tcatctaagc acaccatacg agccagacgc 540
gcaatactag aaatttacta gtcggtaact cgaaacaccg acatctagct aatctttttg 600
ttttatttgg tttccctttg aaatcttcta atttagcttt catagaaata atctaggtat 660
tttttatttt atatgttcta tctgtttgca ttaattttga tcatttgatc tgaatgctgt 720
ggtcacgaga atcgagtgtt tcatggcctt aaaacactcg attatgccat ctgacccgtt 780
ttcaaccatt ctagtgtttc tgagctatat caatggtgca gcatgttagt atacatatct 840
aactattact ccgtatatga gtgagttgtt aaatttattc caggtgaaat ggcattaacg 900
atagccaata ggcggctaaa ttaatagcca tactctaaca gctctaaaaa acatatattc 960
atcgaggcac ctttatgcaa ccacatagtc aacttcaacg tcgcttgcgt gcgttctcaa 1020
gttttctttc ttgcaaatta catttttttt aaaaaaaagt ataatttgta tcgtgcgatt 1080
ttttctctct aggtgtgcgt gactgtggga gtaacaattt tgaatctcaa gaaggaaata 1140
aaagaataat actgctgcct actttgagga tttcagtatt tttctctaaa atgttttggt 1200
gtgatatcta aaccgtcttt aaagccaatt gctcaagatt cattcaacaa ttgaaacgtc 1260
tcacatgact aaatgatata aggttgctaa ggtctttctt gataagcttt tttatgaatt 1320
tcatctaaat tttcgagtga aactattaaa tactaaggtt gctaagtgtc attctcgctc 1380
gagaagtcta acgctttaaa ctttaaccaa atatatacaa gaaaatatta atatttatag 1440
tacataatta gtatcattag atagatcgtt gaatctattt tcataacaaa cttatttgaa 1500
gaaacaaatg ttgttcatat atttctatat acgaatacca tagcgacact tattttagaa 1560
tgtagggagt actccctttg tgccgctttg agtgtcgctt tggcagctag tacctatgtc 1620
caccttcaca gcttgtgcct agtacctaga ctctttctct gtccacattc atttaatctc 1680
tgttgtacct tgttcggaga taaaacgact ctgataaagg gacgaggaag tagtatgtta 1740
gaggagtgaa gtctactccc tttgccgcaa aaaggtaatc ctaagtgtga attgtattct 1800
tttttgacca aaggaatata caacaagaat gatgtcatca tcatgcttcg atcctttttt 1860
ttggtaaagc ttgagcttct gtaaaaatag agaaatcatg ggaaaaatca cgttttggtg 1920
gttttgattt ctagcctcca caataacttt ggttttacta ttttttgttt gattttagtt 1980
tcagaagtcc acttttgtac gtgctcgtag agcctaaaca aaaggctttc caaaacgacc 2040
ttatcttcga gtgttgtaaa aaaaatgagc ccgtttaacg gcgtcgacaa gtctaacgga 2100
caccaaccag cgaaccacca gcgccgagcc aagcgaagca gactgcagac ggcacggccg 2160
agacgttgac accttggcgc ggcaacggca tctctctggc cccctctcga gagttccgct 2220
ccacctccgc atccacctcc acctccacct ccaccggtgg cggtttccaa gtccgtcccg 2280
ttccgccacc tgctcctctc acacggcacg aaaccgtcac ggcaccggca gcacagcacg 2340
ggggattcct ttcccaccgc tccgtccctt tctcttcctc gcccgcccgt tataaatagc 2400
cagccccatc cctcgtctct cgtgttgttc ggagcgcaca cacaacccga tccccaatca 2460
atcgatcccc gcttcaaggt acggcgatcc tcctccctct ctctctacct tctcttctct 2520
acactagatc ggcggtccat ggttagggcc tgctagttcc gttcctgttt ttccatggct 2580
gcgaggtaca atagatctga tggcgttatg atggttaact tgtcatgctt ttgcgattta 2640
tagtcccttt agatagttcg agatcggtga tccatggtta gtaccctagg ctgtggagtc 2700
gggttagatc cgcgctgtta gggttcgtat atggaggcga gctgttctga ttgttaactt 2760
gctgggaatc ctgggatggt tctagctgtt ccgcagatga gatcgatttc atgatctgct 2820
gtatctatcc gtggtatgat gttagccttt gatatggttc gatcgtgcta gctacgtcct 2880
gtgcacttaa ttgtcaggtc ataattttta ctatactttt tttttggttt ggtttggttt 2940
cgtctgattt ggctgtcgtt ctagatcaga gtagaaactg tttcaaacta cctgttggat 3000
ttattaaggt agcgtttggt tcctggtatc gaatcataca cgcaccagtg catcttggat 3060
agccagctgg ggcccacctg tccaaccgtt tggttgccgg atcgaacgag tccattcaag 3120
accgaaccat gcagagcaat cgaatattct cttgtgacgc tgtatcatcc agttcggcaa 3180
aaaacaccga atgccgccat acaggacacc gtactgagcg tctgcaactc tgcatcccgc 3240
tcactgctca catctccgct tgccgcctca cccatccgac tcagaccaga gccacacgga 3300
ttactgctgc tggtgtgtgt attaacaaaa gatccatttg accggagcac atgcagcttg 3360
gatggaaaaa atttattata ttcgtcagtg ctgcatatgt actcatactt gcatgatggt 3420
tttatttatt cgacctcatc agtcctggca ctatggaaag tcattgtagt atagattttt 3480
taatataata taaatcattg gtgacttatc ttgcttaatt ttattttctt attatgaaat 3540
atcgttgcat tcataatagc aaatttgtgc aaatatatag aatctacgtg aaattcttgg 3600
ttggaccaat acaacaaacc cctcaaacat tctcttgtac tgaaccatac cattccgtac 3660
aaccatccaa acaaaaatca tgtatcatca tgtacatgta accaaacaat taacacgcac 3720
catcctattc agacttgtct catccataat ctatccatcc aggatgatcc atcccattca 3780
tctatataca cccaatcaaa cgctacctaa aatttggatc tgtatgtgtc acatatatct 3840
taataagatg gatggaaata tctctttatc ttttagatat ggataggtat atatgttgct 3900
gtgggtttgt tagttatata tatacgtgct tacatacgtg aagaaacctg ctgctacagt 3960
ttaataattc ttgttcatct caacaaataa cgataggcgt atatgttgct gtgtttttta 4020
ctggtacttt gttagatata tacatgctta catacatgaa gaacacatgc tacagttcaa 4080
aaattcttgt tcatctcata aacaaaaagg aggtgtatat gttgctgtgg gttttactgg 4140
tactttatta gatatataca tgcttacata gatgaagcaa catgctgcta tggtgtttaa 4200
taattattgt ttatctaata aacaaacatg ctttttaatt atcttgatat gtttggatga 4260
tggcatatgc agcagctatg tgtggatttt aaatacccag catcatgagc atgcatgacc 4320
ctgccttagt atgcagttat ttgcttgaga ctgtttcttt tgttgatact catcctttag 4380
ttcggtcact cttctgcagg tg 4402
<210> 67
<211> 2423
<212> DNA
<213> Miscanthus sinesis
<400> 67
cacgtggtca ctatgctgca tactccctat acagccgctg accagtacgc tggttcaccg 60
cgtcgcccgc gcgggacgga atgggatgtc acgacccgct gaacgccggg gcatggcatc 120
agcggcgaac aggcacccgg cgtggagctg tccgtgtcac catctacagt gttgacggga 180
cccgcataaa aggagaaaaa aggcccgacg gtcctggaag ccttcctctc cttagctctt 240
ctccctcttt ctctctgtgt aacctgctct tccccttcgt ctataaaaag ggaagtagga 300
cgtcccagga agagaagggc ggttcaccac tctacatggc tatagacata aaaacacacg 360
ccttgggagc acactcacat cagagacttg ggacctatcc ctctctcgct cgtttgtaac 420
ccctactaca aacttttagt gctagtaaca cgagcagcag cgacgaacta gacgtaagga 480
ctttctgccc gaaccagtat aaacatcgtg tcatctaagc acaccatacg agccagacgc 540
gcaatactag aaatttacta gtcggtaact cgaaacaccg acatctagct aatctttttg 600
ttttatttgg tttccctttg aaatcttcta atttagcttt catagaaata atctaggtat 660
tttttatttt atatgttcta tctgtttgca ttaattttga tcatttgatc tgaatgctgt 720
ggtcacgaga atcgagtgtt tcatggcctt aaaacactcg attatgccat ctgacccgtt 780
ttcaaccatt ctagtgtttc tgagctatat caatggtgca gcatgttagt atacatatct 840
aactattact ccgtatatga gtgagttgtt aaatttattc caggtgaaat ggcattaacg 900
atagccaata ggcggctaaa ttaatagcca tactctaaca gctctaaaaa acatatattc 960
atcgaggcac ctttatgcaa ccacatagtc aacttcaacg tcgcttgcgt gcgttctcaa 1020
gttttctttc ttgcaaatta catttttttt aaaaaaaagt ataatttgta tcgtgcgatt 1080
ttttctctct aggtgtgcgt gactgtggga gtaacaattt tgaatctcaa gaaggaaata 1140
aaagaataat actgctgcct actttgagga tttcagtatt tttctctaaa atgttttggt 1200
gtgatatcta aaccgtcttt aaagccaatt gctcaagatt cattcaacaa ttgaaacgtc 1260
tcacatgact aaatgatata aggttgctaa ggtctttctt gataagcttt tttatgaatt 1320
tcatctaaat tttcgagtga aactattaaa tactaaggtt gctaagtgtc attctcgctc 1380
gagaagtcta acgctttaaa ctttaaccaa atatatacaa gaaaatatta atatttatag 1440
tacataatta gtatcattag atagatcgtt gaatctattt tcataacaaa cttatttgaa 1500
gaaacaaatg ttgttcatat atttctatat acgaatacca tagcgacact tattttagaa 1560
tgtagggagt actccctttg tgccgctttg agtgtcgctt tggcagctag tacctatgtc 1620
caccttcaca gcttgtgcct agtacctaga ctctttctct gtccacattc atttaatctc 1680
tgttgtacct tgttcggaga taaaacgact ctgataaagg gacgaggaag tagtatgtta 1740
gaggagtgaa gtctactccc tttgccgcaa aaaggtaatc ctaagtgtga attgtattct 1800
tttttgacca aaggaatata caacaagaat gatgtcatca tcatgcttcg atcctttttt 1860
ttggtaaagc ttgagcttct gtaaaaatag agaaatcatg ggaaaaatca cgttttggtg 1920
gttttgattt ctagcctcca caataacttt ggttttacta ttttttgttt gattttagtt 1980
tcagaagtcc acttttgtac gtgctcgtag agcctaaaca aaaggctttc caaaacgacc 2040
ttatcttcga gtgttgtaaa aaaaatgagc ccgtttaacg gcgtcgacaa gtctaacgga 2100
caccaaccag cgaaccacca gcgccgagcc aagcgaagca gactgcagac ggcacggccg 2160
agacgttgac accttggcgc ggcaacggca tctctctggc cccctctcga gagttccgct 2220
ccacctccgc atccacctcc acctccacct ccaccggtgg cggtttccaa gtccgtcccg 2280
ttccgccacc tgctcctctc acacggcacg aaaccgtcac ggcaccggca gcacagcacg 2340
ggggattcct ttcccaccgc tccgtccctt tctcttcctc gcccgcccgt tataaatagc 2400
cagccccatc cctcgtctct cgt 2423
<210> 68
<211> 55
<212> DNA
<213> Miscanthus sinesis
<400> 68
gttgttcgga gcgcacacac aacccgatcc ccaatcaatc gatccccgct tcaag 55
<210> 69
<211> 1924
<212> DNA
<213> Miscanthus sinesis
<400> 69
gtacggcgat cctcctccct ctctctctac cttctcttct ctacactaga tcggcggtcc 60
atggttaggg cctgctagtt ccgttcctgt ttttccatgg ctgcgaggta caatagatct 120
gatggcgtta tgatggttaa cttgtcatgc ttttgcgatt tatagtccct ttagatagtt 180
cgagatcggt gatccatggt tagtacccta ggctgtggag tcgggttaga tccgcgctgt 240
tagggttcgt atatggaggc gagctgttct gattgttaac ttgctgggaa tcctgggatg 300
gttctagctg ttccgcagat gagatcgatt tcatgatctg ctgtatctat ccgtggtatg 360
atgttagcct ttgatatggt tcgatcgtgc tagctacgtc ctgtgcactt aattgtcagg 420
tcataatttt tactatactt tttttttggt ttggtttggt ttcgtctgat ttggctgtcg 480
ttctagatca gagtagaaac tgtttcaaac tacctgttgg atttattaag gtagcgtttg 540
gttcctggta tcgaatcata cacgcaccag tgcatcttgg atagccagct ggggcccacc 600
tgtccaaccg tttggttgcc ggatcgaacg agtccattca agaccgaacc atgcagagca 660
atcgaatatt ctcttgtgac gctgtatcat ccagttcggc aaaaaacacc gaatgccgcc 720
atacaggaca ccgtactgag cgtctgcaac tctgcatccc gctcactgct cacatctccg 780
cttgccgcct cacccatccg actcagacca gagccacacg gattactgct gctggtgtgt 840
gtattaacaa aagatccatt tgaccggagc acatgcagct tggatggaaa aaatttatta 900
tattcgtcag tgctgcatat gtactcatac ttgcatgatg gttttattta ttcgacctca 960
tcagtcctgg cactatggaa agtcattgta gtatagattt tttaatataa tataaatcat 1020
tggtgactta tcttgcttaa ttttattttc ttattatgaa atatcgttgc attcataata 1080
gcaaatttgt gcaaatatat agaatctacg tgaaattctt ggttggacca atacaacaaa 1140
cccctcaaac attctcttgt actgaaccat accattccgt acaaccatcc aaacaaaaat 1200
catgtatcat catgtacatg taaccaaaca attaacacgc accatcctat tcagacttgt 1260
ctcatccata atctatccat ccaggatgat ccatcccatt catctatata cacccaatca 1320
aacgctacct aaaatttgga tctgtatgtg tcacatatat cttaataaga tggatggaaa 1380
tatctcttta tcttttagat atggataggt atatatgttg ctgtgggttt gttagttata 1440
tatatacgtg cttacatacg tgaagaaacc tgctgctaca gtttaataat tcttgttcat 1500
ctcaacaaat aacgataggc gtatatgttg ctgtgttttt tactggtact ttgttagata 1560
tatacatgct tacatacatg aagaacacat gctacagttc aaaaattctt gttcatctca 1620
taaacaaaaa ggaggtgtat atgttgctgt gggttttact ggtactttat tagatatata 1680
catgcttaca tagatgaagc aacatgctgc tatggtgttt aataattatt gtttatctaa 1740
taaacaaaca tgctttttaa ttatcttgat atgtttggat gatggcatat gcagcagcta 1800
tgtgtggatt ttaaataccc agcatcatga gcatgcatga ccctgcctta gtatgcagtt 1860
atttgcttga gactgtttct tttgttgata ctcatccttt agttcggtca ctcttctgca 1920
ggtg 1924
<210> 70
<211> 3426
<212> DNA
<213> Miscanthus sinesis
<400> 70
gcaaccacat agtcaacttc aacgtcgctt gcgtgcgttc tcaagttttc tttcttgcaa 60
attacatttt ttttaaaaaa aagtataatt tgtatcgtgc gattttttct ctctaggtgt 120
gcgtgactgt gggagtaaca attttgaatc tcaagaagga aataaaagaa taatactgct 180
gcctactttg aggatttcag tatttttctc taaaatgttt tggtgtgata tctaaaccgt 240
ctttaaagcc aattgctcaa gattcattca acaattgaaa cgtctcacat gactaaatga 300
tataaggttg ctaaggtctt tcttgataag cttttttatg aatttcatct aaattttcga 360
gtgaaactat taaatactaa ggttgctaag tgtcattctc gctcgagaag tctaacgctt 420
taaactttaa ccaaatatat acaagaaaat attaatattt atagtacata attagtatca 480
ttagatagat cgttgaatct attttcataa caaacttatt tgaagaaaca aatgttgttc 540
atatatttct atatacgaat accatagcga cacttatttt agaatgtagg gagtactccc 600
tttgtgccgc tttgagtgtc gctttggcag ctagtaccta tgtccacctt cacagcttgt 660
gcctagtacc tagactcttt ctctgtccac attcatttaa tctctgttgt accttgttcg 720
gagataaaac gactctgata aagggacgag gaagtagtat gttagaggag tgaagtctac 780
tccctttgcc gcaaaaaggt aatcctaagt gtgaattgta ttcttttttg accaaaggaa 840
tatacaacaa gaatgatgtc atcatcatgc ttcgatcctt ttttttggta aagcttgagc 900
ttctgtaaaa atagagaaat catgggaaaa atcacgtttt ggtggttttg atttctagcc 960
tccacaataa ctttggtttt actatttttt gtttgatttt agtttcagaa gtccactttt 1020
gtacgtgctc gtagagccta aacaaaaggc tttccaaaac gaccttatct tcgagtgttg 1080
taaaaaaaat gagcccgttt aacggcgtcg acaagtctaa cggacaccaa ccagcgaacc 1140
accagcgccg agccaagcga agcagactgc agacggcacg gccgagacgt tgacaccttg 1200
gcgcggcaac ggcatctctc tggccccctc tcgagagttc cgctccacct ccgcatccac 1260
ctccacctcc acctccaccg gtggcggttt ccaagtccgt cccgttccgc cacctgctcc 1320
tctcacacgg cacgaaaccg tcacggcacc ggcagcacag cacgggggat tcctttccca 1380
ccgctccgtc cctttctctt cctcgcccgc ccgttataaa tagccagccc catccctcgt 1440
ctctcgtgtt gttcggagcg cacacacaac ccgatcccca atcaatcgat ccccgcttca 1500
aggtacggcg atcctcctcc ctctctctct accttctctt ctctacacta gatcggcggt 1560
ccatggttag ggcctgctag ttccgttcct gtttttccat ggctgcgagg tacaatagat 1620
ctgatggcgt tatgatggtt aacttgtcat gcttttgcga tttatagtcc ctttagatag 1680
ttcgagatcg gtgatccatg gttagtaccc taggctgtgg agtcgggtta gatccgcgct 1740
gttagggttc gtatatggag gcgagctgtt ctgattgtta acttgctggg aatcctggga 1800
tggttctagc tgttccgcag atgagatcga tttcatgatc tgctgtatct atccgtggta 1860
tgatgttagc ctttgatatg gttcgatcgt gctagctacg tcctgtgcac ttaattgtca 1920
ggtcataatt tttactatac tttttttttg gtttggtttg gtttcgtctg atttggctgt 1980
cgttctagat cagagtagaa actgtttcaa actacctgtt ggatttatta aggtagcgtt 2040
tggttcctgg tatcgaatca tacacgcacc agtgcatctt ggatagccag ctggggccca 2100
cctgtccaac cgtttggttg ccggatcgaa cgagtccatt caagaccgaa ccatgcagag 2160
caatcgaata ttctcttgtg acgctgtatc atccagttcg gcaaaaaaca ccgaatgccg 2220
ccatacagga caccgtactg agcgtctgca actctgcatc ccgctcactg ctcacatctc 2280
cgcttgccgc ctcacccatc cgactcagac cagagccaca cggattactg ctgctggtgt 2340
gtgtattaac aaaagatcca tttgaccgga gcacatgcag cttggatgga aaaaatttat 2400
tatattcgtc agtgctgcat atgtactcat acttgcatga tggttttatt tattcgacct 2460
catcagtcct ggcactatgg aaagtcattg tagtatagat tttttaatat aatataaatc 2520
attggtgact tatcttgctt aattttattt tcttattatg aaatatcgtt gcattcataa 2580
tagcaaattt gtgcaaatat atagaatcta cgtgaaattc ttggttggac caatacaaca 2640
aacccctcaa acattctctt gtactgaacc ataccattcc gtacaaccat ccaaacaaaa 2700
atcatgtatc atcatgtaca tgtaaccaaa caattaacac gcaccatcct attcagactt 2760
gtctcatcca taatctatcc atccaggatg atccatccca ttcatctata tacacccaat 2820
caaacgctac ctaaaatttg gatctgtatg tgtcacatat atcttaataa gatggatgga 2880
aatatctctt tatcttttag atatggatag gtatatatgt tgctgtgggt ttgttagtta 2940
tatatatacg tgcttacata cgtgaagaaa cctgctgcta cagtttaata attcttgttc 3000
atctcaacaa ataacgatag gcgtatatgt tgctgtgttt tttactggta ctttgttaga 3060
tatatacatg cttacataca tgaagaacac atgctacagt tcaaaaattc ttgttcatct 3120
cataaacaaa aaggaggtgt atatgttgct gtgggtttta ctggtacttt attagatata 3180
tacatgctta catagatgaa gcaacatgct gctatggtgt ttaataatta ttgtttatct 3240
aataaacaaa catgcttttt aattatcttg atatgtttgg atgatggcat atgcagcagc 3300
tatgtgtgga ttttaaatac ccagcatcat gagcatgcat gaccctgcct tagtatgcag 3360
ttatttgctt gagactgttt cttttgttga tactcatcct ttagttcggt cactcttctg 3420
caggtg 3426
<210> 71
<211> 1447
<212> DNA
<213> Miscanthus sinesis
<400> 71
gcaaccacat agtcaacttc aacgtcgctt gcgtgcgttc tcaagttttc tttcttgcaa 60
attacatttt ttttaaaaaa aagtataatt tgtatcgtgc gattttttct ctctaggtgt 120
gcgtgactgt gggagtaaca attttgaatc tcaagaagga aataaaagaa taatactgct 180
gcctactttg aggatttcag tatttttctc taaaatgttt tggtgtgata tctaaaccgt 240
ctttaaagcc aattgctcaa gattcattca acaattgaaa cgtctcacat gactaaatga 300
tataaggttg ctaaggtctt tcttgataag cttttttatg aatttcatct aaattttcga 360
gtgaaactat taaatactaa ggttgctaag tgtcattctc gctcgagaag tctaacgctt 420
taaactttaa ccaaatatat acaagaaaat attaatattt atagtacata attagtatca 480
ttagatagat cgttgaatct attttcataa caaacttatt tgaagaaaca aatgttgttc 540
atatatttct atatacgaat accatagcga cacttatttt agaatgtagg gagtactccc 600
tttgtgccgc tttgagtgtc gctttggcag ctagtaccta tgtccacctt cacagcttgt 660
gcctagtacc tagactcttt ctctgtccac attcatttaa tctctgttgt accttgttcg 720
gagataaaac gactctgata aagggacgag gaagtagtat gttagaggag tgaagtctac 780
tccctttgcc gcaaaaaggt aatcctaagt gtgaattgta ttcttttttg accaaaggaa 840
tatacaacaa gaatgatgtc atcatcatgc ttcgatcctt ttttttggta aagcttgagc 900
ttctgtaaaa atagagaaat catgggaaaa atcacgtttt ggtggttttg atttctagcc 960
tccacaataa ctttggtttt actatttttt gtttgatttt agtttcagaa gtccactttt 1020
gtacgtgctc gtagagccta aacaaaaggc tttccaaaac gaccttatct tcgagtgttg 1080
taaaaaaaat gagcccgttt aacggcgtcg acaagtctaa cggacaccaa ccagcgaacc 1140
accagcgccg agccaagcga agcagactgc agacggcacg gccgagacgt tgacaccttg 1200
gcgcggcaac ggcatctctc tggccccctc tcgagagttc cgctccacct ccgcatccac 1260
ctccacctcc acctccaccg gtggcggttt ccaagtccgt cccgttccgc cacctgctcc 1320
tctcacacgg cacgaaaccg tcacggcacc ggcagcacag cacgggggat tcctttccca 1380
ccgctccgtc cctttctctt cctcgcccgc ccgttataaa tagccagccc catccctcgt 1440
ctctcgt 1447
<210> 72
<211> 2878
<212> DNA
<213> Miscanthus sinesis
<400> 72
ctatatacga ataccatagc gacacttatt ttagaatgta gggagtactc cctttgtgcc 60
gctttgagtg tcgctttggc agctagtacc tatgtccacc ttcacagctt gtgcctagta 120
cctagactct ttctctgtcc acattcattt aatctctgtt gtaccttgtt cggagataaa 180
acgactctga taaagggacg aggaagtagt atgttagagg agtgaagtct actccctttg 240
ccgcaaaaag gtaatcctaa gtgtgaattg tattcttttt tgaccaaagg aatatacaac 300
aagaatgatg tcatcatcat gcttcgatcc ttttttttgg taaagcttga gcttctgtaa 360
aaatagagaa atcatgggaa aaatcacgtt ttggtggttt tgatttctag cctccacaat 420
aactttggtt ttactatttt ttgtttgatt ttagtttcag aagtccactt ttgtacgtgc 480
tcgtagagcc taaacaaaag gctttccaaa acgaccttat cttcgagtgt tgtaaaaaaa 540
atgagcccgt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa ccaccagcgc 600
cgagccaagc gaagcagact gcagacggca cggccgagac gttgacacct tggcgcggca 660
acggcatctc tctggccccc tctcgagagt tccgctccac ctccgcatcc acctccacct 720
ccacctccac cggtggcggt ttccaagtcc gtcccgttcc gccacctgct cctctcacac 780
ggcacgaaac cgtcacggca ccggcagcac agcacggggg attcctttcc caccgctccg 840
tccctttctc ttcctcgccc gcccgttata aatagccagc cccatccctc gtctctcgtg 900
ttgttcggag cgcacacaca acccgatccc caatcaatcg atccccgctt caaggtacgg 960
cgatcctcct ccctctctct ctaccttctc ttctctacac tagatcggcg gtccatggtt 1020
agggcctgct agttccgttc ctgtttttcc atggctgcga ggtacaatag atctgatggc 1080
gttatgatgg ttaacttgtc atgcttttgc gatttatagt ccctttagat agttcgagat 1140
cggtgatcca tggttagtac cctaggctgt ggagtcgggt tagatccgcg ctgttagggt 1200
tcgtatatgg aggcgagctg ttctgattgt taacttgctg ggaatcctgg gatggttcta 1260
gctgttccgc agatgagatc gatttcatga tctgctgtat ctatccgtgg tatgatgtta 1320
gcctttgata tggttcgatc gtgctagcta cgtcctgtgc acttaattgt caggtcataa 1380
tttttactat actttttttt tggtttggtt tggtttcgtc tgatttggct gtcgttctag 1440
atcagagtag aaactgtttc aaactacctg ttggatttat taaggtagcg tttggttcct 1500
ggtatcgaat catacacgca ccagtgcatc ttggatagcc agctggggcc cacctgtcca 1560
accgtttggt tgccggatcg aacgagtcca ttcaagaccg aaccatgcag agcaatcgaa 1620
tattctcttg tgacgctgta tcatccagtt cggcaaaaaa caccgaatgc cgccatacag 1680
gacaccgtac tgagcgtctg caactctgca tcccgctcac tgctcacatc tccgcttgcc 1740
gcctcaccca tccgactcag accagagcca cacggattac tgctgctggt gtgtgtatta 1800
acaaaagatc catttgaccg gagcacatgc agcttggatg gaaaaaattt attatattcg 1860
tcagtgctgc atatgtactc atacttgcat gatggtttta tttattcgac ctcatcagtc 1920
ctggcactat ggaaagtcat tgtagtatag attttttaat ataatataaa tcattggtga 1980
cttatcttgc ttaattttat tttcttatta tgaaatatcg ttgcattcat aatagcaaat 2040
ttgtgcaaat atatagaatc tacgtgaaat tcttggttgg accaatacaa caaacccctc 2100
aaacattctc ttgtactgaa ccataccatt ccgtacaacc atccaaacaa aaatcatgta 2160
tcatcatgta catgtaacca aacaattaac acgcaccatc ctattcagac ttgtctcatc 2220
cataatctat ccatccagga tgatccatcc cattcatcta tatacaccca atcaaacgct 2280
acctaaaatt tggatctgta tgtgtcacat atatcttaat aagatggatg gaaatatctc 2340
tttatctttt agatatggat aggtatatat gttgctgtgg gtttgttagt tatatatata 2400
cgtgcttaca tacgtgaaga aacctgctgc tacagtttaa taattcttgt tcatctcaac 2460
aaataacgat aggcgtatat gttgctgtgt tttttactgg tactttgtta gatatataca 2520
tgcttacata catgaagaac acatgctaca gttcaaaaat tcttgttcat ctcataaaca 2580
aaaaggaggt gtatatgttg ctgtgggttt tactggtact ttattagata tatacatgct 2640
tacatagatg aagcaacatg ctgctatggt gtttaataat tattgtttat ctaataaaca 2700
aacatgcttt ttaattatct tgatatgttt ggatgatggc atatgcagca gctatgtgtg 2760
gattttaaat acccagcatc atgagcatgc atgaccctgc cttagtatgc agttatttgc 2820
ttgagactgt ttcttttgtt gatactcatc ctttagttcg gtcactcttc tgcaggtg 2878
<210> 73
<211> 899
<212> DNA
<213> Miscanthus sinesis
<400> 73
ctatatacga ataccatagc gacacttatt ttagaatgta gggagtactc cctttgtgcc 60
gctttgagtg tcgctttggc agctagtacc tatgtccacc ttcacagctt gtgcctagta 120
cctagactct ttctctgtcc acattcattt aatctctgtt gtaccttgtt cggagataaa 180
acgactctga taaagggacg aggaagtagt atgttagagg agtgaagtct actccctttg 240
ccgcaaaaag gtaatcctaa gtgtgaattg tattcttttt tgaccaaagg aatatacaac 300
aagaatgatg tcatcatcat gcttcgatcc ttttttttgg taaagcttga gcttctgtaa 360
aaatagagaa atcatgggaa aaatcacgtt ttggtggttt tgatttctag cctccacaat 420
aactttggtt ttactatttt ttgtttgatt ttagtttcag aagtccactt ttgtacgtgc 480
tcgtagagcc taaacaaaag gctttccaaa acgaccttat cttcgagtgt tgtaaaaaaa 540
atgagcccgt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa ccaccagcgc 600
cgagccaagc gaagcagact gcagacggca cggccgagac gttgacacct tggcgcggca 660
acggcatctc tctggccccc tctcgagagt tccgctccac ctccgcatcc acctccacct 720
ccacctccac cggtggcggt ttccaagtcc gtcccgttcc gccacctgct cctctcacac 780
ggcacgaaac cgtcacggca ccggcagcac agcacggggg attcctttcc caccgctccg 840
tccctttctc ttcctcgccc gcccgttata aatagccagc cccatccctc gtctctcgt 899
<210> 74
<211> 2670
<212> DNA
<213> Miscanthus sinesis
<400> 74
gtatgttaga ggagtgaagt ctactccctt tgccgcaaaa aggtaatcct aagtgtgaat 60
tgtattcttt tttgaccaaa ggaatataca acaagaatga tgtcatcatc atgcttcgat 120
cctttttttt ggtaaagctt gagcttctgt aaaaatagag aaatcatggg aaaaatcacg 180
ttttggtggt tttgatttct agcctccaca ataactttgg ttttactatt ttttgtttga 240
ttttagtttc agaagtccac ttttgtacgt gctcgtagag cctaaacaaa aggctttcca 300
aaacgacctt atcttcgagt gttgtaaaaa aaatgagccc gtttaacggc gtcgacaagt 360
ctaacggaca ccaaccagcg aaccaccagc gccgagccaa gcgaagcaga ctgcagacgg 420
cacggccgag acgttgacac cttggcgcgg caacggcatc tctctggccc cctctcgaga 480
gttccgctcc acctccgcat ccacctccac ctccacctcc accggtggcg gtttccaagt 540
ccgtcccgtt ccgccacctg ctcctctcac acggcacgaa accgtcacgg caccggcagc 600
acagcacggg ggattccttt cccaccgctc cgtccctttc tcttcctcgc ccgcccgtta 660
taaatagcca gccccatccc tcgtctctcg tgttgttcgg agcgcacaca caacccgatc 720
cccaatcaat cgatccccgc ttcaaggtac ggcgatcctc ctccctctct ctctaccttc 780
tcttctctac actagatcgg cggtccatgg ttagggcctg ctagttccgt tcctgttttt 840
ccatggctgc gaggtacaat agatctgatg gcgttatgat ggttaacttg tcatgctttt 900
gcgatttata gtccctttag atagttcgag atcggtgatc catggttagt accctaggct 960
gtggagtcgg gttagatccg cgctgttagg gttcgtatat ggaggcgagc tgttctgatt 1020
gttaacttgc tgggaatcct gggatggttc tagctgttcc gcagatgaga tcgatttcat 1080
gatctgctgt atctatccgt ggtatgatgt tagcctttga tatggttcga tcgtgctagc 1140
tacgtcctgt gcacttaatt gtcaggtcat aatttttact atactttttt tttggtttgg 1200
tttggtttcg tctgatttgg ctgtcgttct agatcagagt agaaactgtt tcaaactacc 1260
tgttggattt attaaggtag cgtttggttc ctggtatcga atcatacacg caccagtgca 1320
tcttggatag ccagctgggg cccacctgtc caaccgtttg gttgccggat cgaacgagtc 1380
cattcaagac cgaaccatgc agagcaatcg aatattctct tgtgacgctg tatcatccag 1440
ttcggcaaaa aacaccgaat gccgccatac aggacaccgt actgagcgtc tgcaactctg 1500
catcccgctc actgctcaca tctccgcttg ccgcctcacc catccgactc agaccagagc 1560
cacacggatt actgctgctg gtgtgtgtat taacaaaaga tccatttgac cggagcacat 1620
gcagcttgga tggaaaaaat ttattatatt cgtcagtgct gcatatgtac tcatacttgc 1680
atgatggttt tatttattcg acctcatcag tcctggcact atggaaagtc attgtagtat 1740
agatttttta atataatata aatcattggt gacttatctt gcttaatttt attttcttat 1800
tatgaaatat cgttgcattc ataatagcaa atttgtgcaa atatatagaa tctacgtgaa 1860
attcttggtt ggaccaatac aacaaacccc tcaaacattc tcttgtactg aaccatacca 1920
ttccgtacaa ccatccaaac aaaaatcatg tatcatcatg tacatgtaac caaacaatta 1980
acacgcacca tcctattcag acttgtctca tccataatct atccatccag gatgatccat 2040
cccattcatc tatatacacc caatcaaacg ctacctaaaa tttggatctg tatgtgtcac 2100
atatatctta ataagatgga tggaaatatc tctttatctt ttagatatgg ataggtatat 2160
atgttgctgt gggtttgtta gttatatata tacgtgctta catacgtgaa gaaacctgct 2220
gctacagttt aataattctt gttcatctca acaaataacg ataggcgtat atgttgctgt 2280
gttttttact ggtactttgt tagatatata catgcttaca tacatgaaga acacatgcta 2340
cagttcaaaa attcttgttc atctcataaa caaaaaggag gtgtatatgt tgctgtgggt 2400
tttactggta ctttattaga tatatacatg cttacataga tgaagcaaca tgctgctatg 2460
gtgtttaata attattgttt atctaataaa caaacatgct ttttaattat cttgatatgt 2520
ttggatgatg gcatatgcag cagctatgtg tggattttaa atacccagca tcatgagcat 2580
gcatgaccct gccttagtat gcagttattt gcttgagact gtttcttttg ttgatactca 2640
tcctttagtt cggtcactct tctgcaggtg 2670
<210> 75
<211> 691
<212> DNA
<213> Miscanthus sinesis
<400> 75
gtatgttaga ggagtgaagt ctactccctt tgccgcaaaa aggtaatcct aagtgtgaat 60
tgtattcttt tttgaccaaa ggaatataca acaagaatga tgtcatcatc atgcttcgat 120
cctttttttt ggtaaagctt gagcttctgt aaaaatagag aaatcatggg aaaaatcacg 180
ttttggtggt tttgatttct agcctccaca ataactttgg ttttactatt ttttgtttga 240
ttttagtttc agaagtccac ttttgtacgt gctcgtagag cctaaacaaa aggctttcca 300
aaacgacctt atcttcgagt gttgtaaaaa aaatgagccc gtttaacggc gtcgacaagt 360
ctaacggaca ccaaccagcg aaccaccagc gccgagccaa gcgaagcaga ctgcagacgg 420
cacggccgag acgttgacac cttggcgcgg caacggcatc tctctggccc cctctcgaga 480
gttccgctcc acctccgcat ccacctccac ctccacctcc accggtggcg gtttccaagt 540
ccgtcccgtt ccgccacctg ctcctctcac acggcacgaa accgtcacgg caccggcagc 600
acagcacggg ggattccttt cccaccgctc cgtccctttc tcttcctcgc ccgcccgtta 660
taaatagcca gccccatccc tcgtctctcg t 691
<210> 76
<211> 2485
<212> DNA
<213> Miscanthus sinesis
<400> 76
gtggttttga tttctagcct ccacaataac tttggtttta ctattttttg tttgatttta 60
gtttcagaag tccacttttg tacgtgctcg tagagcctaa acaaaaggct ttccaaaacg 120
accttatctt cgagtgttgt aaaaaaaatg agcccgttta acggcgtcga caagtctaac 180
ggacaccaac cagcgaacca ccagcgccga gccaagcgaa gcagactgca gacggcacgg 240
ccgagacgtt gacaccttgg cgcggcaacg gcatctctct ggccccctct cgagagttcc 300
gctccacctc cgcatccacc tccacctcca cctccaccgg tggcggtttc caagtccgtc 360
ccgttccgcc acctgctcct ctcacacggc acgaaaccgt cacggcaccg gcagcacagc 420
acgggggatt cctttcccac cgctccgtcc ctttctcttc ctcgcccgcc cgttataaat 480
agccagcccc atccctcgtc tctcgtgttg ttcggagcgc acacacaacc cgatccccaa 540
tcaatcgatc cccgcttcaa ggtacggcga tcctcctccc tctctctcta ccttctcttc 600
tctacactag atcggcggtc catggttagg gcctgctagt tccgttcctg tttttccatg 660
gctgcgaggt acaatagatc tgatggcgtt atgatggtta acttgtcatg cttttgcgat 720
ttatagtccc tttagatagt tcgagatcgg tgatccatgg ttagtaccct aggctgtgga 780
gtcgggttag atccgcgctg ttagggttcg tatatggagg cgagctgttc tgattgttaa 840
cttgctggga atcctgggat ggttctagct gttccgcaga tgagatcgat ttcatgatct 900
gctgtatcta tccgtggtat gatgttagcc tttgatatgg ttcgatcgtg ctagctacgt 960
cctgtgcact taattgtcag gtcataattt ttactatact ttttttttgg tttggtttgg 1020
tttcgtctga tttggctgtc gttctagatc agagtagaaa ctgtttcaaa ctacctgttg 1080
gatttattaa ggtagcgttt ggttcctggt atcgaatcat acacgcacca gtgcatcttg 1140
gatagccagc tggggcccac ctgtccaacc gtttggttgc cggatcgaac gagtccattc 1200
aagaccgaac catgcagagc aatcgaatat tctcttgtga cgctgtatca tccagttcgg 1260
caaaaaacac cgaatgccgc catacaggac accgtactga gcgtctgcaa ctctgcatcc 1320
cgctcactgc tcacatctcc gcttgccgcc tcacccatcc gactcagacc agagccacac 1380
ggattactgc tgctggtgtg tgtattaaca aaagatccat ttgaccggag cacatgcagc 1440
ttggatggaa aaaatttatt atattcgtca gtgctgcata tgtactcata cttgcatgat 1500
ggttttattt attcgacctc atcagtcctg gcactatgga aagtcattgt agtatagatt 1560
ttttaatata atataaatca ttggtgactt atcttgctta attttatttt cttattatga 1620
aatatcgttg cattcataat agcaaatttg tgcaaatata tagaatctac gtgaaattct 1680
tggttggacc aatacaacaa acccctcaaa cattctcttg tactgaacca taccattccg 1740
tacaaccatc caaacaaaaa tcatgtatca tcatgtacat gtaaccaaac aattaacacg 1800
caccatccta ttcagacttg tctcatccat aatctatcca tccaggatga tccatcccat 1860
tcatctatat acacccaatc aaacgctacc taaaatttgg atctgtatgt gtcacatata 1920
tcttaataag atggatggaa atatctcttt atcttttaga tatggatagg tatatatgtt 1980
gctgtgggtt tgttagttat atatatacgt gcttacatac gtgaagaaac ctgctgctac 2040
agtttaataa ttcttgttca tctcaacaaa taacgatagg cgtatatgtt gctgtgtttt 2100
ttactggtac tttgttagat atatacatgc ttacatacat gaagaacaca tgctacagtt 2160
caaaaattct tgttcatctc ataaacaaaa aggaggtgta tatgttgctg tgggttttac 2220
tggtacttta ttagatatat acatgcttac atagatgaag caacatgctg ctatggtgtt 2280
taataattat tgtttatcta ataaacaaac atgcttttta attatcttga tatgtttgga 2340
tgatggcata tgcagcagct atgtgtggat tttaaatacc cagcatcatg agcatgcatg 2400
accctgcctt agtatgcagt tatttgcttg agactgtttc ttttgttgat actcatcctt 2460
tagttcggtc actcttctgc aggtg 2485
<210> 77
<211> 506
<212> DNA
<213> Miscanthus sinesis
<400> 77
gtggttttga tttctagcct ccacaataac tttggtttta ctattttttg tttgatttta 60
gtttcagaag tccacttttg tacgtgctcg tagagcctaa acaaaaggct ttccaaaacg 120
accttatctt cgagtgttgt aaaaaaaatg agcccgttta acggcgtcga caagtctaac 180
ggacaccaac cagcgaacca ccagcgccga gccaagcgaa gcagactgca gacggcacgg 240
ccgagacgtt gacaccttgg cgcggcaacg gcatctctct ggccccctct cgagagttcc 300
gctccacctc cgcatccacc tccacctcca cctccaccgg tggcggtttc caagtccgtc 360
ccgttccgcc acctgctcct ctcacacggc acgaaaccgt cacggcaccg gcagcacagc 420
acgggggatt cctttcccac cgctccgtcc ctttctcttc ctcgcccgcc cgttataaat 480
agccagcccc atccctcgtc tctcgt 506
<210> 78
<211> 4079
<212> DNA
<213> Schizachyium scoparium
<400> 78
ctctatgcct gtgtcattgt gccagcccct acctctgtca atgttcaaga tccaaataag 60
agaatgggat agaccatata ttaatggtgt agtatgcatc aagatctgaa tattatatga 120
gtgaattgat aaatttattc taggtgacat ggccttaacg atggccagta cgtggttaaa 180
tcaatgaatc aatagccata ctctaatagc tctaaaaaag gatatatatt tgtcgaggca 240
ctattatgca accacatagt caacttcaaa gccgcttgag tgcgttctaa aaaaaaaatt 300
tcttgtaaat tacgcttttc tcaaaaaaat tggatcatgc atttatttca ctctaggtgt 360
gcgtgactac gtgaataaca attttgaatc tcagcaagga aataaaagta taataccgct 420
gtctactttg aagatcacaa tatctttctc ttagaatgtt tctgtttgtt atttaaaacc 480
atcgttatta aggtcaaatg atcaagattc attcaacaat tgaaacttct cacatgatta 540
catcatatat aaggttgcta aggtcttgct tgacaaggta tctctagtaa catctagttt 600
ttttgagtga aataataaaa ttttaaagca atgttacaag agaagctctg gagataaaag 660
ttagaagggt gaagtttact ccctctatcc caaagatgta attctaagaa tgacttaaat 720
tttttataca aaaggagtat atatcacaag attgatgtca tcgttatgct taggccacgt 780
acacgacgct ggcgcttatg tggacgttaa tcggtaattc ttcattttat tttattttgt 840
tgtcaccgcg tacatttggg ttaggcgttt gttaaaggca ttgccactca aacaagcagc 900
cgcgtttgga gcttttatag tttgaaaagt gacggttgta aagatgagta agctgattat 960
tagtagagta aattataatt atcatacaac aactctcaaa gtgggtgcac gttagtccaa 1020
catcttataa tttatccaac tcaatacaac aactatatag gtgggtgcat gttggtccaa 1080
catcttctaa tttgtttaat ttgatacgag aacttgtctt attggtacat atatgatcca 1140
aagcattgta acaacgtgtt tatgtatact cttaatcatg gtcatcagaa gctaacacac 1200
acgctcatgc catccatatc attcaacttt tgaatcgttt actatacaat attatttcta 1260
aatttggctg taaagatggc attgatttca taaatatgaa aaataccaaa ttgcacattt 1320
tctttctata ttataatatt gttttcatct attttcaccc cgtaaccttt aatttggtca 1380
tttagggctc actaaaactg atatgtgggt tgtgcatcgc ataagaatca agaacccaga 1440
agtaattttc aatactaaga aacaacaaaa tttggttttt ttttgtttgg tttcgattat 1500
agccgaacta accaaattta agaaagcttt ttatatttgg ccacataaga aatgatatca 1560
tttaatattg taactgattc aagctgagta atagatgaga tgagtgtgtt aggatgtgta 1620
gcttccgatg atagagaatt agagtgtaca aagacgcatc gttacaatat ttggacctta 1680
tatgcaccaa tgtgtcaagt ctcgcttcaa attaactata ttaaaagatg ttggatcaac 1740
atgcactcac ttagatatca gtcgtattaa attgaacaaa ttacaagata ttggactatg 1800
cacccactca aatagttgtt atatagtgaa tacagtttac tcttagtagt atatgtaagt 1860
tcagcctttt ctattgtagg ttaagcctta attaaggctc ttacacaatt gtttcattat 1920
tcgcgttcga agcagcttct tcgtagattt tgcgagggaa ggctgcctcg gttttgcctt 1980
ccctagcact catgtgagag cctctggcaa taggtcttct catttttatt cacattcttt 2040
aagagcccat ataagcgttc atgacttgta tatactctta gatctttttt tgtgggtaaa 2100
gctcaagcta atctaaaaat agagaaatca ggaacaaaga atcatgtttt ggtggttttg 2160
atttctagcc tccacaataa ttttagttta cctttttttg tttgatttta attttagaag 2220
ggtttatagc aggacttaaa atccaaaatg accattatct tcgagtaata acccgtttaa 2280
cggcgtcgac aagtctaacg gacaccaacc catgaaccac cagcgccgag ccaagaactg 2340
aaggtcgaga cgttgacacc tttggcgcga cacggcatgt tggcatctcc ctctctggcc 2400
ccctctcgag aattccgctc caccgcctca accggagacg gtttccaaag ttgtgcttag 2460
atgctcaaaa gttggtgaaa tcatttttat ttggcaattt gtgtccaact atagactaat 2520
taggctcaaa agatttgtct cgtaaagtac attcaaactg tgtaattagt tattttattt 2580
atctacattt aatactctat gaatgcgtca agagatttga tgtgacttta atgtgacgga 2640
caatctgaaa cttttacgca acttgcatat aaacagagcc caagtccgtt ccgttccgtt 2700
ccgcttcctc ctcccagacg gcacgaaacc gtgacggcac cggcagcacg gggattcctt 2760
tcccaccgct ccttcctttt cccttcatcg cccgcagcta taaatagcca cccccgtccg 2820
caacttcttt ccccaacctc atcttttgtt cggagcacgc acacaatccg atcgatcccc 2880
aatcccctcg tctctcctcg cgagcctcgt cgatccgcca ttcaaggtac ggcgatcatc 2940
ctccctccct ctctacctgc tcttctgtag atcggcgacc ccatccatgg ttagggcctg 3000
ctagttctgt tcctgttttt tttccatggc tgcgaggtag aatagatctg atggcgttat 3060
gatggttaat ttgtcatact cttgcggtct atgggtccct ttaggtcatc aatttaattt 3120
tgggtggttg agatcggtga tccatggtta gtaccctagt cagtggggtt ggatccgtgc 3180
tattagggtt cgtagatgga ttctgatggc tcagtaactg ggaatcctag gatggttcca 3240
tctggtttgc agatgagaac gatttcatca tctgctatat cttgtttcgt tgcgtaggtt 3300
ctgtttaaac taatccgtgg tatgatgtta gcctttgata aggttgattt catcatctgc 3360
tatatcttgt ttcgttgcgt aggttctgtt taaactaatc cgtggtatga tgttagcctt 3420
tgataaggtt tgattgtgct agctacgtcc tgtgcagcag ttaattgtca ggtcatacgt 3480
cataattttt agcatgtctg tttttgtttg atttcgttgt ctgattaggc tgtagatagt 3540
ttcgatctac ctgtcggttt attttattaa aatttggatc cgtatgtgtg tcacatatat 3600
cttcatgatt aagatggagt tatatgggta ggttatacat gtggctgtgg atcatgatta 3660
agatggattg aagtatctct ttatctttta gttaggatag attattatat atgttgctgt 3720
tgattttatt ggttctttat tatatatatt catgcttata tacataaaag caatgtgcta 3780
ttacagttta atagttcttg attatctaat aaacaaataa ggataggtat atttgttgct 3840
gttggtttta ctggtactct attagatagt actttgacat gaagcaacat cctgctatgg 3900
attaataatt attcttcgtc taataaaaag catggttttt aattattttg atttgatata 3960
cttggatgat gtcatatgca gcagctattt gtgaattttt cggccgtatc ttcatattgc 4020
ttgggactgt ttctttggtt gataactcac cctgttgttt ggtgatcctt ctgcaggtg 4079
<210> 79
<211> 2831
<212> DNA
<213> Schizachyium scoparium
<400> 79
ctctatgcct gtgtcattgt gccagcccct acctctgtca atgttcaaga tccaaataag 60
agaatgggat agaccatata ttaatggtgt agtatgcatc aagatctgaa tattatatga 120
gtgaattgat aaatttattc taggtgacat ggccttaacg atggccagta cgtggttaaa 180
tcaatgaatc aatagccata ctctaatagc tctaaaaaag gatatatatt tgtcgaggca 240
ctattatgca accacatagt caacttcaaa gccgcttgag tgcgttctaa aaaaaaaatt 300
tcttgtaaat tacgcttttc tcaaaaaaat tggatcatgc atttatttca ctctaggtgt 360
gcgtgactac gtgaataaca attttgaatc tcagcaagga aataaaagta taataccgct 420
gtctactttg aagatcacaa tatctttctc ttagaatgtt tctgtttgtt atttaaaacc 480
atcgttatta aggtcaaatg atcaagattc attcaacaat tgaaacttct cacatgatta 540
catcatatat aaggttgcta aggtcttgct tgacaaggta tctctagtaa catctagttt 600
ttttgagtga aataataaaa ttttaaagca atgttacaag agaagctctg gagataaaag 660
ttagaagggt gaagtttact ccctctatcc caaagatgta attctaagaa tgacttaaat 720
tttttataca aaaggagtat atatcacaag attgatgtca tcgttatgct taggccacgt 780
acacgacgct ggcgcttatg tggacgttaa tcggtaattc ttcattttat tttattttgt 840
tgtcaccgcg tacatttggg ttaggcgttt gttaaaggca ttgccactca aacaagcagc 900
cgcgtttgga gcttttatag tttgaaaagt gacggttgta aagatgagta agctgattat 960
tagtagagta aattataatt atcatacaac aactctcaaa gtgggtgcac gttagtccaa 1020
catcttataa tttatccaac tcaatacaac aactatatag gtgggtgcat gttggtccaa 1080
catcttctaa tttgtttaat ttgatacgag aacttgtctt attggtacat atatgatcca 1140
aagcattgta acaacgtgtt tatgtatact cttaatcatg gtcatcagaa gctaacacac 1200
acgctcatgc catccatatc attcaacttt tgaatcgttt actatacaat attatttcta 1260
aatttggctg taaagatggc attgatttca taaatatgaa aaataccaaa ttgcacattt 1320
tctttctata ttataatatt gttttcatct attttcaccc cgtaaccttt aatttggtca 1380
tttagggctc actaaaactg atatgtgggt tgtgcatcgc ataagaatca agaacccaga 1440
agtaattttc aatactaaga aacaacaaaa tttggttttt ttttgtttgg tttcgattat 1500
agccgaacta accaaattta agaaagcttt ttatatttgg ccacataaga aatgatatca 1560
tttaatattg taactgattc aagctgagta atagatgaga tgagtgtgtt aggatgtgta 1620
gcttccgatg atagagaatt agagtgtaca aagacgcatc gttacaatat ttggacctta 1680
tatgcaccaa tgtgtcaagt ctcgcttcaa attaactata ttaaaagatg ttggatcaac 1740
atgcactcac ttagatatca gtcgtattaa attgaacaaa ttacaagata ttggactatg 1800
cacccactca aatagttgtt atatagtgaa tacagtttac tcttagtagt atatgtaagt 1860
tcagcctttt ctattgtagg ttaagcctta attaaggctc ttacacaatt gtttcattat 1920
tcgcgttcga agcagcttct tcgtagattt tgcgagggaa ggctgcctcg gttttgcctt 1980
ccctagcact catgtgagag cctctggcaa taggtcttct catttttatt cacattcttt 2040
aagagcccat ataagcgttc atgacttgta tatactctta gatctttttt tgtgggtaaa 2100
gctcaagcta atctaaaaat agagaaatca ggaacaaaga atcatgtttt ggtggttttg 2160
atttctagcc tccacaataa ttttagttta cctttttttg tttgatttta attttagaag 2220
ggtttatagc aggacttaaa atccaaaatg accattatct tcgagtaata acccgtttaa 2280
cggcgtcgac aagtctaacg gacaccaacc catgaaccac cagcgccgag ccaagaactg 2340
aaggtcgaga cgttgacacc tttggcgcga cacggcatgt tggcatctcc ctctctggcc 2400
ccctctcgag aattccgctc caccgcctca accggagacg gtttccaaag ttgtgcttag 2460
atgctcaaaa gttggtgaaa tcatttttat ttggcaattt gtgtccaact atagactaat 2520
taggctcaaa agatttgtct cgtaaagtac attcaaactg tgtaattagt tattttattt 2580
atctacattt aatactctat gaatgcgtca agagatttga tgtgacttta atgtgacgga 2640
caatctgaaa cttttacgca acttgcatat aaacagagcc caagtccgtt ccgttccgtt 2700
ccgcttcctc ctcccagacg gcacgaaacc gtgacggcac cggcagcacg gggattcctt 2760
tcccaccgct ccttcctttt cccttcatcg cccgcagcta taaatagcca cccccgtccg 2820
caacttcttt c 2831
<210> 80
<211> 95
<212> DNA
<213> Schizachyium scoparium
<400> 80
cccaacctca tcttttgttc ggagcacgca cacaatccga tcgatcccca atcccctcgt 60
ctctcctcgc gagcctcgtc gatccgccat tcaag 95
<210> 81
<211> 1153
<212> DNA
<213> Schizachyium scoparium
<400> 81
gtacggcgat catcctccct ccctctctac ctgctcttct gtagatcggc gaccccatcc 60
atggttaggg cctgctagtt ctgttcctgt tttttttcca tggctgcgag gtagaataga 120
tctgatggcg ttatgatggt taatttgtca tactcttgcg gtctatgggt ccctttaggt 180
catcaattta attttgggtg gttgagatcg gtgatccatg gttagtaccc tagtcagtgg 240
ggttggatcc gtgctattag ggttcgtaga tggattctga tggctcagta actgggaatc 300
ctaggatggt tccatctggt ttgcagatga gaacgatttc atcatctgct atatcttgtt 360
tcgttgcgta ggttctgttt aaactaatcc gtggtatgat gttagccttt gataaggttg 420
atttcatcat ctgctatatc ttgtttcgtt gcgtaggttc tgtttaaact aatccgtggt 480
atgatgttag cctttgataa ggtttgattg tgctagctac gtcctgtgca gcagttaatt 540
gtcaggtcat acgtcataat ttttagcatg tctgtttttg tttgatttcg ttgtctgatt 600
aggctgtaga tagtttcgat ctacctgtcg gtttatttta ttaaaatttg gatccgtatg 660
tgtgtcacat atatcttcat gattaagatg gagttatatg ggtaggttat acatgtggct 720
gtggatcatg attaagatgg attgaagtat ctctttatct tttagttagg atagattatt 780
atatatgttg ctgttgattt tattggttct ttattatata tattcatgct tatatacata 840
aaagcaatgt gctattacag tttaatagtt cttgattatc taataaacaa ataaggatag 900
gtatatttgt tgctgttggt tttactggta ctctattaga tagtactttg acatgaagca 960
acatcctgct atggattaat aattattctt cgtctaataa aaagcatggt ttttaattat 1020
tttgatttga tatacttgga tgatgtcata tgcagcagct atttgtgaat ttttcggccg 1080
tatcttcata ttgcttggga ctgtttcttt ggttgataac tcaccctgtt gtttggtgat 1140
ccttctgcag gtg 1153
<210> 82
<211> 3281
<212> DNA
<213> Schizachyium scoparium
<400> 82
gtggacgtta atcggtaatt cttcatttta ttttattttg ttgtcaccgc gtacatttgg 60
gttaggcgtt tgttaaaggc attgccactc aaacaagcag ccgcgtttgg agcttttata 120
gtttgaaaag tgacggttgt aaagatgagt aagctgatta ttagtagagt aaattataat 180
tatcatacaa caactctcaa agtgggtgca cgttagtcca acatcttata atttatccaa 240
ctcaatacaa caactatata ggtgggtgca tgttggtcca acatcttcta atttgtttaa 300
tttgatacga gaacttgtct tattggtaca tatatgatcc aaagcattgt aacaacgtgt 360
ttatgtatac tcttaatcat ggtcatcaga agctaacaca cacgctcatg ccatccatat 420
cattcaactt ttgaatcgtt tactatacaa tattatttct aaatttggct gtaaagatgg 480
cattgatttc ataaatatga aaaataccaa attgcacatt ttctttctat attataatat 540
tgttttcatc tattttcacc ccgtaacctt taatttggtc atttagggct cactaaaact 600
gatatgtggg ttgtgcatcg cataagaatc aagaacccag aagtaatttt caatactaag 660
aaacaacaaa atttggtttt tttttgtttg gtttcgatta tagccgaact aaccaaattt 720
aagaaagctt tttatatttg gccacataag aaatgatatc atttaatatt gtaactgatt 780
caagctgagt aatagatgag atgagtgtgt taggatgtgt agcttccgat gatagagaat 840
tagagtgtac aaagacgcat cgttacaata tttggacctt atatgcacca atgtgtcaag 900
tctcgcttca aattaactat attaaaagat gttggatcaa catgcactca cttagatatc 960
agtcgtatta aattgaacaa attacaagat attggactat gcacccactc aaatagttgt 1020
tatatagtga atacagttta ctcttagtag tatatgtaag ttcagccttt tctattgtag 1080
gttaagcctt aattaaggct cttacacaat tgtttcatta ttcgcgttcg aagcagcttc 1140
ttcgtagatt ttgcgaggga aggctgcctc ggttttgcct tccctagcac tcatgtgaga 1200
gcctctggca ataggtcttc tcatttttat tcacattctt taagagccca tataagcgtt 1260
catgacttgt atatactctt agatcttttt tttgtgggta aagctcaagc taatctaaaa 1320
atagagaaat caggaacaaa gaatcatgtt ttggtggttt tgatttctag cctccacaat 1380
aattttagtt tacctttttt tgtttgattt taattttaga agggtttata gcaggactta 1440
aaatccaaaa tgaccattat cttcgagtaa taacccgttt aacggcgtcg acaagtctaa 1500
cggacaccaa cccatgaacc accagcgccg agccaagaac tgaaggtcga gacgttgaca 1560
cctttggcgc gacacggcat gttggcatct ccctctctgg ccccctctcg agaattccgc 1620
tccaccgcct caaccggaga cggtttccaa agttgtgctt agatgctcaa aagttggtga 1680
aatcattttt atttggcaat ttgtgtccaa ctatagacta attaggctca aaagatttgt 1740
ctcgtaaagt acattcaaac tgtgtaatta gttattttat ttatctacat ttaatactct 1800
atgaatgcgt caagagattt gatgtgactt taatgtgacg gacaatctga aacttttacg 1860
caacttgcat ataaacagag cccaagtccg ttccgttccg ttccgcttcc tcctcccaga 1920
cggcacgaaa ccgtgacggc accggcagca cggggattcc tttcccaccg ctccttcctt 1980
ttcccttcat cgcccgcagc tataaatagc cacccccgtc cgcaacttct ttccccaacc 2040
tcatcttttg ttcggagcac gcacacaatc cgatcgatcc ccaatcccct cgtctctcct 2100
cgcgagcctc gtcgatccgc cattcaaggt acggcgatca tcctccctcc ctctctacct 2160
gctcttctgt agatcggcga ccccatccat ggttagggcc tgctagttct gttcctgttt 2220
tttttccatg gctgcgaggt agaatagatc tgatggcgtt atgatggtta atttgtcata 2280
ctcttgcggt ctatgggtcc ctttaggtca tcaatttaat tttgggtggt tgagatcggt 2340
gatccatggt tagtacccta gtcagtgggg ttggatccgt gctattaggg ttcgtagatg 2400
gattctgatg gctcagtaac tgggaatcct aggatggttc catctggttt gcagatgaga 2460
acgatttcat catctgctat atcttgtttc gttgcgtagg ttctgtttaa actaatccgt 2520
ggtatgatgt tagcctttga taaggttgat ttcatcatct gctatatctt gtttcgttgc 2580
gtaggttctg tttaaactaa tccgtggtat gatgttagcc tttgataagg tttgattgtg 2640
ctagctacgt cctgtgcagc agttaattgt caggtcatac gtcataattt ttagcatgtc 2700
tgtttttgtt tgatttcgtt gtctgattag gctgtagata gtttcgatct acctgtcggt 2760
ttattttatt aaaatttgga tccgtatgtg tgtcacatat atcttcatga ttaagatgga 2820
gttatatggg taggttatac atgtggctgt ggatcatgat taagatggat tgaagtatct 2880
ctttatcttt tagttaggat agattattat atatgttgct gttgatttta ttggttcttt 2940
attatatata ttcatgctta tatacataaa agcaatgtgc tattacagtt taatagttct 3000
tgattatcta ataaacaaat aaggataggt atatttgttg ctgttggttt tactggtact 3060
ctattagata gtactttgac atgaagcaac atcctgctat ggattaataa ttattcttcg 3120
tctaataaaa agcatggttt ttaattattt tgatttgata tacttggatg atgtcatatg 3180
cagcagctat ttgtgaattt ttcggccgta tcttcatatt gcttgggact gtttctttgg 3240
ttgataactc accctgttgt ttggtgatcc ttctgcaggt g 3281
<210> 83
<211> 2033
<212> DNA
<213> Schizachyium scoparium
<400> 83
gtggacgtta atcggtaatt cttcatttta ttttattttg ttgtcaccgc gtacatttgg 60
gttaggcgtt tgttaaaggc attgccactc aaacaagcag ccgcgtttgg agcttttata 120
gtttgaaaag tgacggttgt aaagatgagt aagctgatta ttagtagagt aaattataat 180
tatcatacaa caactctcaa agtgggtgca cgttagtcca acatcttata atttatccaa 240
ctcaatacaa caactatata ggtgggtgca tgttggtcca acatcttcta atttgtttaa 300
tttgatacga gaacttgtct tattggtaca tatatgatcc aaagcattgt aacaacgtgt 360
ttatgtatac tcttaatcat ggtcatcaga agctaacaca cacgctcatg ccatccatat 420
cattcaactt ttgaatcgtt tactatacaa tattatttct aaatttggct gtaaagatgg 480
cattgatttc ataaatatga aaaataccaa attgcacatt ttctttctat attataatat 540
tgttttcatc tattttcacc ccgtaacctt taatttggtc atttagggct cactaaaact 600
gatatgtggg ttgtgcatcg cataagaatc aagaacccag aagtaatttt caatactaag 660
aaacaacaaa atttggtttt tttttgtttg gtttcgatta tagccgaact aaccaaattt 720
aagaaagctt tttatatttg gccacataag aaatgatatc atttaatatt gtaactgatt 780
caagctgagt aatagatgag atgagtgtgt taggatgtgt agcttccgat gatagagaat 840
tagagtgtac aaagacgcat cgttacaata tttggacctt atatgcacca atgtgtcaag 900
tctcgcttca aattaactat attaaaagat gttggatcaa catgcactca cttagatatc 960
agtcgtatta aattgaacaa attacaagat attggactat gcacccactc aaatagttgt 1020
tatatagtga atacagttta ctcttagtag tatatgtaag ttcagccttt tctattgtag 1080
gttaagcctt aattaaggct cttacacaat tgtttcatta ttcgcgttcg aagcagcttc 1140
ttcgtagatt ttgcgaggga aggctgcctc ggttttgcct tccctagcac tcatgtgaga 1200
gcctctggca ataggtcttc tcatttttat tcacattctt taagagccca tataagcgtt 1260
catgacttgt atatactctt agatcttttt tttgtgggta aagctcaagc taatctaaaa 1320
atagagaaat caggaacaaa gaatcatgtt ttggtggttt tgatttctag cctccacaat 1380
aattttagtt tacctttttt tgtttgattt taattttaga agggtttata gcaggactta 1440
aaatccaaaa tgaccattat cttcgagtaa taacccgttt aacggcgtcg acaagtctaa 1500
cggacaccaa cccatgaacc accagcgccg agccaagaac tgaaggtcga gacgttgaca 1560
cctttggcgc gacacggcat gttggcatct ccctctctgg ccccctctcg agaattccgc 1620
tccaccgcct caaccggaga cggtttccaa agttgtgctt agatgctcaa aagttggtga 1680
aatcattttt atttggcaat ttgtgtccaa ctatagacta attaggctca aaagatttgt 1740
ctcgtaaagt acattcaaac tgtgtaatta gttattttat ttatctacat ttaatactct 1800
atgaatgcgt caagagattt gatgtgactt taatgtgacg gacaatctga aacttttacg 1860
caacttgcat ataaacagag cccaagtccg ttccgttccg ttccgcttcc tcctcccaga 1920
cggcacgaaa ccgtgacggc accggcagca cggggattcc tttcccaccg ctccttcctt 1980
ttcccttcat cgcccgcagc tataaatagc cacccccgtc cgcaacttct ttc 2033
<210> 84
<211> 2294
<212> DNA
<213> Schizachyium scoparium
<400> 84
gatattggac tatgcaccca ctcaaatagt tgttatatag tgaatacagt ttactcttag 60
tagtatatgt aagttcagcc ttttctattg taggttaagc cttaattaag gctcttacac 120
aattgtttca ttattcgcgt tcgaagcagc ttcttcgtag attttgcgag ggaaggctgc 180
ctcggttttg ccttccctag cactcatgtg agagcctctg gcaataggtc ttctcatttt 240
tattcacatt ctttaagagc ccatataagc gttcatgact tgtatatact cttagatctt 300
ttttttgtgg gtaaagctca agctaatcta aaaatagaga aatcaggaac aaagaatcat 360
gttttggtgg ttttgatttc tagcctccac aataatttta gtttaccttt ttttgtttga 420
ttttaatttt agaagggttt atagcaggac ttaaaatcca aaatgaccat tatcttcgag 480
taataacccg tttaacggcg tcgacaagtc taacggacac caacccatga accaccagcg 540
ccgagccaag aactgaaggt cgagacgttg acacctttgg cgcgacacgg catgttggca 600
tctccctctc tggccccctc tcgagaattc cgctccaccg cctcaaccgg agacggtttc 660
caaagttgtg cttagatgct caaaagttgg tgaaatcatt tttatttggc aatttgtgtc 720
caactataga ctaattaggc tcaaaagatt tgtctcgtaa agtacattca aactgtgtaa 780
ttagttattt tatttatcta catttaatac tctatgaatg cgtcaagaga tttgatgtga 840
ctttaatgtg acggacaatc tgaaactttt acgcaacttg catataaaca gagcccaagt 900
ccgttccgtt ccgttccgct tcctcctccc agacggcacg aaaccgtgac ggcaccggca 960
gcacggggat tcctttccca ccgctccttc cttttccctt catcgcccgc agctataaat 1020
agccaccccc gtccgcaact tctttcccca acctcatctt ttgttcggag cacgcacaca 1080
atccgatcga tccccaatcc cctcgtctct cctcgcgagc ctcgtcgatc cgccattcaa 1140
ggtacggcga tcatcctccc tccctctcta cctgctcttc tgtagatcgg cgaccccatc 1200
catggttagg gcctgctagt tctgttcctg ttttttttcc atggctgcga ggtagaatag 1260
atctgatggc gttatgatgg ttaatttgtc atactcttgc ggtctatggg tccctttagg 1320
tcatcaattt aattttgggt ggttgagatc ggtgatccat ggttagtacc ctagtcagtg 1380
gggttggatc cgtgctatta gggttcgtag atggattctg atggctcagt aactgggaat 1440
cctaggatgg ttccatctgg tttgcagatg agaacgattt catcatctgc tatatcttgt 1500
ttcgttgcgt aggttctgtt taaactaatc cgtggtatga tgttagcctt tgataaggtt 1560
gatttcatca tctgctatat cttgtttcgt tgcgtaggtt ctgtttaaac taatccgtgg 1620
tatgatgtta gcctttgata aggtttgatt gtgctagcta cgtcctgtgc agcagttaat 1680
tgtcaggtca tacgtcataa tttttagcat gtctgttttt gtttgatttc gttgtctgat 1740
taggctgtag atagtttcga tctacctgtc ggtttatttt attaaaattt ggatccgtat 1800
gtgtgtcaca tatatcttca tgattaagat ggagttatat gggtaggtta tacatgtggc 1860
tgtggatcat gattaagatg gattgaagta tctctttatc ttttagttag gatagattat 1920
tatatatgtt gctgttgatt ttattggttc tttattatat atattcatgc ttatatacat 1980
aaaagcaatg tgctattaca gtttaatagt tcttgattat ctaataaaca aataaggata 2040
ggtatatttg ttgctgttgg ttttactggt actctattag atagtacttt gacatgaagc 2100
aacatcctgc tatggattaa taattattct tcgtctaata aaaagcatgg tttttaatta 2160
ttttgatttg atatacttgg atgatgtcat atgcagcagc tatttgtgaa tttttcggcc 2220
gtatcttcat attgcttggg actgtttctt tggttgataa ctcaccctgt tgtttggtga 2280
tccttctgca ggtg 2294
<210> 85
<211> 1046
<212> DNA
<213> Schizachyium scoparium
<400> 85
gatattggac tatgcaccca ctcaaatagt tgttatatag tgaatacagt ttactcttag 60
tagtatatgt aagttcagcc ttttctattg taggttaagc cttaattaag gctcttacac 120
aattgtttca ttattcgcgt tcgaagcagc ttcttcgtag attttgcgag ggaaggctgc 180
ctcggttttg ccttccctag cactcatgtg agagcctctg gcaataggtc ttctcatttt 240
tattcacatt ctttaagagc ccatataagc gttcatgact tgtatatact cttagatctt 300
ttttttgtgg gtaaagctca agctaatcta aaaatagaga aatcaggaac aaagaatcat 360
gttttggtgg ttttgatttc tagcctccac aataatttta gtttaccttt ttttgtttga 420
ttttaatttt agaagggttt atagcaggac ttaaaatcca aaatgaccat tatcttcgag 480
taataacccg tttaacggcg tcgacaagtc taacggacac caacccatga accaccagcg 540
ccgagccaag aactgaaggt cgagacgttg acacctttgg cgcgacacgg catgttggca 600
tctccctctc tggccccctc tcgagaattc cgctccaccg cctcaaccgg agacggtttc 660
caaagttgtg cttagatgct caaaagttgg tgaaatcatt tttatttggc aatttgtgtc 720
caactataga ctaattaggc tcaaaagatt tgtctcgtaa agtacattca aactgtgtaa 780
ttagttattt tatttatcta catttaatac tctatgaatg cgtcaagaga tttgatgtga 840
ctttaatgtg acggacaatc tgaaactttt acgcaacttg catataaaca gagcccaagt 900
ccgttccgtt ccgttccgct tcctcctccc agacggcacg aaaccgtgac ggcaccggca 960
gcacggggat tcctttccca ccgctccttc cttttccctt catcgcccgc agctataaat 1020
agccaccccc gtccgcaact tctttc 1046
<210> 86
<211> 1795
<212> DNA
<213> Schizachyium scoparium
<400> 86
gtcgacaagt ctaacggaca ccaacccatg aaccaccagc gccgagccaa gaactgaagg 60
tcgagacgtt gacacctttg gcgcgacacg gcatgttggc atctccctct ctggccccct 120
ctcgagaatt ccgctccacc gcctcaaccg gagacggttt ccaaagttgt gcttagatgc 180
tcaaaagttg gtgaaatcat ttttatttgg caatttgtgt ccaactatag actaattagg 240
ctcaaaagat ttgtctcgta aagtacattc aaactgtgta attagttatt ttatttatct 300
acatttaata ctctatgaat gcgtcaagag atttgatgtg actttaatgt gacggacaat 360
ctgaaacttt tacgcaactt gcatataaac agagcccaag tccgttccgt tccgttccgc 420
ttcctcctcc cagacggcac gaaaccgtga cggcaccggc agcacgggga ttcctttccc 480
accgctcctt ccttttccct tcatcgcccg cagctataaa tagccacccc cgtccgcaac 540
ttctttcccc aacctcatct tttgttcgga gcacgcacac aatccgatcg atccccaatc 600
ccctcgtctc tcctcgcgag cctcgtcgat ccgccattca aggtacggcg atcatcctcc 660
ctccctctct acctgctctt ctgtagatcg gcgaccccat ccatggttag ggcctgctag 720
ttctgttcct gttttttttc catggctgcg aggtagaata gatctgatgg cgttatgatg 780
gttaatttgt catactcttg cggtctatgg gtccctttag gtcatcaatt taattttggg 840
tggttgagat cggtgatcca tggttagtac cctagtcagt ggggttggat ccgtgctatt 900
agggttcgta gatggattct gatggctcag taactgggaa tcctaggatg gttccatctg 960
gtttgcagat gagaacgatt tcatcatctg ctatatcttg tttcgttgcg taggttctgt 1020
ttaaactaat ccgtggtatg atgttagcct ttgataaggt tgatttcatc atctgctata 1080
tcttgtttcg ttgcgtaggt tctgtttaaa ctaatccgtg gtatgatgtt agcctttgat 1140
aaggtttgat tgtgctagct acgtcctgtg cagcagttaa ttgtcaggtc atacgtcata 1200
atttttagca tgtctgtttt tgtttgattt cgttgtctga ttaggctgta gatagtttcg 1260
atctacctgt cggtttattt tattaaaatt tggatccgta tgtgtgtcac atatatcttc 1320
atgattaaga tggagttata tgggtaggtt atacatgtgg ctgtggatca tgattaagat 1380
ggattgaagt atctctttat cttttagtta ggatagatta ttatatatgt tgctgttgat 1440
tttattggtt ctttattata tatattcatg cttatataca taaaagcaat gtgctattac 1500
agtttaatag ttcttgatta tctaataaac aaataaggat aggtatattt gttgctgttg 1560
gttttactgg tactctatta gatagtactt tgacatgaag caacatcctg ctatggatta 1620
ataattattc ttcgtctaat aaaaagcatg gtttttaatt attttgattt gatatacttg 1680
gatgatgtca tatgcagcag ctatttgtga atttttcggc cgtatcttca tattgcttgg 1740
gactgtttct ttggttgata actcaccctg ttgtttggtg atccttctgc aggtg 1795
<210> 87
<211> 547
<212> DNA
<213> Schizachyium scoparium
<400> 87
gtcgacaagt ctaacggaca ccaacccatg aaccaccagc gccgagccaa gaactgaagg 60
tcgagacgtt gacacctttg gcgcgacacg gcatgttggc atctccctct ctggccccct 120
ctcgagaatt ccgctccacc gcctcaaccg gagacggttt ccaaagttgt gcttagatgc 180
tcaaaagttg gtgaaatcat ttttatttgg caatttgtgt ccaactatag actaattagg 240
ctcaaaagat ttgtctcgta aagtacattc aaactgtgta attagttatt ttatttatct 300
acatttaata ctctatgaat gcgtcaagag atttgatgtg actttaatgt gacggacaat 360
ctgaaacttt tacgcaactt gcatataaac agagcccaag tccgttccgt tccgttccgc 420
ttcctcctcc cagacggcac gaaaccgtga cggcaccggc agcacgggga ttcctttccc 480
accgctcctt ccttttccct tcatcgcccg cagctataaa tagccacccc cgtccgcaac 540
ttctttc 547
<210> 88
<211> 3357
<212> DNA
<213> Sorghastrum nutans
<400> 88
gtggccagct tttgttctag ttcaacggtc cgggccttcc gggaacctaa tgcactaatt 60
gattattatt aatctactat tgcagctaac ctcaaaagaa atgctctgca gttagttgtc 120
cgtcccaatc aatccaccag cagactcaca ttattgatgg aggaaattaa attcagcctt 180
tgacgtggat gcaacaactg cacaagatac catctacttt gcttaatttg ctgatgtttt 240
gagaaaatta aaccagcttt gaccaacaca tgagatgggc gccttacgtt tggcacaatg 300
taatgtagtc cggcacggca agttagactc tgtgtgtagt gttatattag ccggcctctt 360
taggtttggc acaatttaat tgaatccggc atggcaagtt agactgcagt gtgagccggt 420
caccgcaagt taggatataa tatacaagag caagtataca ataaagtgac attagcgtaa 480
agttatatga catatggaat ataagagaaa atacggagta tataataagg tgaactgtat 540
agcgatcaaa tttatgctaa gcgaagaaaa gagaagataa ataggttgaa aacttatagt 600
gagctttggc tcataatcta aataattatg agagagtggg atcgaccaca tattcatttt 660
gtagtacgta ctctctccgt tttttataag ttgctttgat ttttttttat atcaattttg 720
ctatacatct aaacataata ggaatatcaa gttcatgaag gtcgtgattt gcactaaata 780
tgttccctta ttagatagac gagttgttta gttttattgt agatgatata gcgcttgcat 840
atagcatgtg aaccggctaa attattagcc atacacgact ataaaaaatg acattccttt 900
gaggaacttt tatgcaacca aatagtcaac ttcaatgttg ctagagcggg ctttaagcca 960
aaagcagctg ctgctttgtt tccgagagaa gggacattct agttgatagc aaaacaaata 1020
cgtagcagtt gtagcgagtg tgtgagtaat aatttttctc tagtgtgtac gagtatgcga 1080
gtaataattt taaatctcta gaaggaagaa aaataatatt gctacctact ttgaggatat 1140
caataccttt ctctaaaatg ttttggtgaa gccatcttta aagctaattg ttcaagattc 1200
aaccattggg acgtctcaaa tgattagatc ctataatact cctacgtact aaattataag 1260
tcgttttgat tttattggta catacatttt gctatgtgtt tagatataat aatatgtcta 1320
gatacattgg atgaaccgaa aaaatcgaaa cgacttataa tttggatcga aaggagtatt 1380
tgctaaagtc cttttcgaag ttccggctct aaatttttgg ataaaatttt atgaaatact 1440
atcttaagaa gtaatttgac tagagaagct tgaagagtat aatctcttaa ttttgtgcta 1500
caggagtgaa gccaacgtcg tatttagatc tagatgctgt caggtagtga ggacggaggg 1560
agtattggat aaagtcattc caagatctta gaaaattaaa gtatattaag tttgattaaa 1620
tttatatgac aagtaataac attcatgatg ccaattaagt atcattagat tcttcatcaa 1680
ctatattttc atagtatact tatttaatgt tataaatttt tataattttt tttataattt 1740
tagctaaact cgagatcgat tcttataatt aaaaataaac tgaaaaaaaa tcacatgttc 1800
aagtgacagg aggagccagt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa 1860
ccaccagcgc cgagccaatc ccaagcgaag ccgactgcag acggccgaga cgttgacacc 1920
tttggcgcgg catccatctc tccggccccc tcttgagagt tccgccccac cggcggcggt 1980
ttccaagtcc gttccgcccg ccttcgcggt tggacttgtt ccggtggcgc ctggcggatc 2040
gcgtggcgga gcggagacga cgaggtgagc cgtgggcgtt cctcctcctg ctcctctcac 2100
acggcacgga acggaaccgt gacggcaccg ggcagcacgg gcgggattcc ttccccacct 2160
ctccttcggt cctccctcca tcataaatag ccacccccct cccaccttct ttccccacct 2220
cgtctcccct cgtgttattc ggagcacaga cacaccccga tccccaatcc tctcctcgcg 2280
agcctcgtcg atccccgctt caaggtacgg cgatcatcct ccctccctaa ctccaatccg 2340
tggttagggc ctgctagatc gtcctccctc cctacctgcg atccgtggtt cgcgcctgct 2400
agttctgttt cctgtttgtc gatggctgcg aggtataata gatctgatgg cgtgcggtgt 2460
gacggttaaa ttcacatgct cttgcgattt atacgcgaat cgatctggga ttgctcgaga 2520
tcggtgatcc atggttagaa ccctaggcgg tggagtcggg ttaaatccgt gctgttaggg 2580
ttcgtaggtg gatgcgacct gttctggttg tttacttgtc agtatttagg aatcctacta 2640
ggatggttct agctggttcg cagatgagat cgatttcatg atctgctata tctttcgttg 2700
cctaagtttc gtttaatctg tccgtggtat gatgttagcc tttgatatgc ttcgatcgtg 2760
ctagctacct cctgtgcact aaattatcag ctcgtaattt ttagcatgcc cttttttttt 2820
tgggtattgt tcgattgagg tgtcgttcta gatcagagta ggaagactgt ttcaaactac 2880
ctgctggatt tattaaattt ggatctgtat gagtatcaca tatatctcca taatttagat 2940
ggatggaaat atcccttttt cttttagata ctgtttggta tagattttgc tgtgggtttt 3000
actggtactt agatactctt cgtttagata tggatatgtt tacatgcaga tacatgaagc 3060
aacatgctgc tacagtttaa tatggatagg tgtatatgtt gttgtgggtc ctttacttac 3120
atgcttagat acatgaagca acatgctgct acgtttaata attattgttt atctgatctg 3180
atttaaacaa acatgctttt taattgtcct gaaatgcttg gatgatggca tatgcagcag 3240
ctatgtgtgg attttaaata cccagcatga gcatgcatga ccctaactta gtatgctgtt 3300
tatttgcttg acttttcttt tgttgatact cacccttttg tttgttgact cttgcag 3357
<210> 89
<211> 2218
<212> DNA
<213> Sorghastrum nutans
<400> 89
gtggccagct tttgttctag ttcaacggtc cgggccttcc gggaacctaa tgcactaatt 60
gattattatt aatctactat tgcagctaac ctcaaaagaa atgctctgca gttagttgtc 120
cgtcccaatc aatccaccag cagactcaca ttattgatgg aggaaattaa attcagcctt 180
tgacgtggat gcaacaactg cacaagatac catctacttt gcttaatttg ctgatgtttt 240
gagaaaatta aaccagcttt gaccaacaca tgagatgggc gccttacgtt tggcacaatg 300
taatgtagtc cggcacggca agttagactc tgtgtgtagt gttatattag ccggcctctt 360
taggtttggc acaatttaat tgaatccggc atggcaagtt agactgcagt gtgagccggt 420
caccgcaagt taggatataa tatacaagag caagtataca ataaagtgac attagcgtaa 480
agttatatga catatggaat ataagagaaa atacggagta tataataagg tgaactgtat 540
agcgatcaaa tttatgctaa gcgaagaaaa gagaagataa ataggttgaa aacttatagt 600
gagctttggc tcataatcta aataattatg agagagtggg atcgaccaca tattcatttt 660
gtagtacgta ctctctccgt tttttataag ttgctttgat ttttttttat atcaattttg 720
ctatacatct aaacataata ggaatatcaa gttcatgaag gtcgtgattt gcactaaata 780
tgttccctta ttagatagac gagttgttta gttttattgt agatgatata gcgcttgcat 840
atagcatgtg aaccggctaa attattagcc atacacgact ataaaaaatg acattccttt 900
gaggaacttt tatgcaacca aatagtcaac ttcaatgttg ctagagcggg ctttaagcca 960
aaagcagctg ctgctttgtt tccgagagaa gggacattct agttgatagc aaaacaaata 1020
cgtagcagtt gtagcgagtg tgtgagtaat aatttttctc tagtgtgtac gagtatgcga 1080
gtaataattt taaatctcta gaaggaagaa aaataatatt gctacctact ttgaggatat 1140
caataccttt ctctaaaatg ttttggtgaa gccatcttta aagctaattg ttcaagattc 1200
aaccattggg acgtctcaaa tgattagatc ctataatact cctacgtact aaattataag 1260
tcgttttgat tttattggta catacatttt gctatgtgtt tagatataat aatatgtcta 1320
gatacattgg atgaaccgaa aaaatcgaaa cgacttataa tttggatcga aaggagtatt 1380
tgctaaagtc cttttcgaag ttccggctct aaatttttgg ataaaatttt atgaaatact 1440
atcttaagaa gtaatttgac tagagaagct tgaagagtat aatctcttaa ttttgtgcta 1500
caggagtgaa gccaacgtcg tatttagatc tagatgctgt caggtagtga ggacggaggg 1560
agtattggat aaagtcattc caagatctta gaaaattaaa gtatattaag tttgattaaa 1620
tttatatgac aagtaataac attcatgatg ccaattaagt atcattagat tcttcatcaa 1680
ctatattttc atagtatact tatttaatgt tataaatttt tataattttt tttataattt 1740
tagctaaact cgagatcgat tcttataatt aaaaataaac tgaaaaaaaa tcacatgttc 1800
aagtgacagg aggagccagt ttaacggcgt cgacaagtct aacggacacc aaccagcgaa 1860
ccaccagcgc cgagccaatc ccaagcgaag ccgactgcag acggccgaga cgttgacacc 1920
tttggcgcgg catccatctc tccggccccc tcttgagagt tccgccccac cggcggcggt 1980
ttccaagtcc gttccgcccg ccttcgcggt tggacttgtt ccggtggcgc ctggcggatc 2040
gcgtggcgga gcggagacga cgaggtgagc cgtgggcgtt cctcctcctg ctcctctcac 2100
acggcacgga acggaaccgt gacggcaccg ggcagcacgg gcgggattcc ttccccacct 2160
ctccttcggt cctccctcca tcataaatag ccacccccct cccaccttct ttccccac 2218
<210> 90
<211> 86
<212> DNA
<213> Sorghastrum nutans
<400> 90
ctcgtctccc ctcgtgttat tcggagcaca gacacacccc gatccccaat cctctcctcg 60
cgagcctcgt cgatccccgc ttcaag 86
<210> 91
<211> 1053
<212> DNA
<213> Sorghastrum nutans
<400> 91
gtacggcgat catcctccct ccctaactcc aatccgtggt tagggcctgc tagatcgtcc 60
tccctcccta cctgcgatcc gtggttcgcg cctgctagtt ctgtttcctg tttgtcgatg 120
gctgcgaggt ataatagatc tgatggcgtg cggtgtgacg gttaaattca catgctcttg 180
cgatttatac gcgaatcgat ctgggattgc tcgagatcgg tgatccatgg ttagaaccct 240
aggcggtgga gtcgggttaa atccgtgctg ttagggttcg taggtggatg cgacctgttc 300
tggttgttta cttgtcagta tttaggaatc ctactaggat ggttctagct ggttcgcaga 360
tgagatcgat ttcatgatct gctatatctt tcgttgccta agtttcgttt aatctgtccg 420
tggtatgatg ttagcctttg atatgcttcg atcgtgctag ctacctcctg tgcactaaat 480
tatcagctcg taatttttag catgcccttt tttttttggg tattgttcga ttgaggtgtc 540
gttctagatc agagtaggaa gactgtttca aactacctgc tggatttatt aaatttggat 600
ctgtatgagt atcacatata tctccataat ttagatggat ggaaatatcc ctttttcttt 660
tagatactgt ttggtataga ttttgctgtg ggttttactg gtacttagat actcttcgtt 720
tagatatgga tatgtttaca tgcagataca tgaagcaaca tgctgctaca gtttaatatg 780
gataggtgta tatgttgttg tgggtccttt acttacatgc ttagatacat gaagcaacat 840
gctgctacgt ttaataatta ttgtttatct gatctgattt aaacaaacat gctttttaat 900
tgtcctgaaa tgcttggatg atggcatatg cagcagctat gtgtggattt taaataccca 960
gcatgagcat gcatgaccct aacttagtat gctgtttatt tgcttgactt ttcttttgtt 1020
gatactcacc cttttgtttg ttgactcttg cag 1053
<210> 92
<211> 3106
<212> DNA
<213> Sorghastrum nutans
<400> 92
agctttgacc aacacatgag atgggcgcct tacgtttggc acaatgtaat gtagtccggc 60
acggcaagtt agactctgtg tgtagtgtta tattagccgg cctctttagg tttggcacaa 120
tttaattgaa tccggcatgg caagttagac tgcagtgtga gccggtcacc gcaagttagg 180
atataatata caagagcaag tatacaataa agtgacatta gcgtaaagtt atatgacata 240
tggaatataa gagaaaatac ggagtatata ataaggtgaa ctgtatagcg atcaaattta 300
tgctaagcga agaaaagaga agataaatag gttgaaaact tatagtgagc tttggctcat 360
aatctaaata attatgagag agtgggatcg accacatatt cattttgtag tacgtactct 420
ctccgttttt tataagttgc tttgattttt ttttatatca attttgctat acatctaaac 480
ataataggaa tatcaagttc atgaaggtcg tgatttgcac taaatatgtt cccttattag 540
atagacgagt tgtttagttt tattgtagat gatatagcgc ttgcatatag catgtgaacc 600
ggctaaatta ttagccatac acgactataa aaaatgacat tcctttgagg aacttttatg 660
caaccaaata gtcaacttca atgttgctag agcgggcttt aagccaaaag cagctgctgc 720
tttgtttccg agagaaggga cattctagtt gatagcaaaa caaatacgta gcagttgtag 780
cgagtgtgtg agtaataatt tttctctagt gtgtacgagt atgcgagtaa taattttaaa 840
tctctagaag gaagaaaaat aatattgcta cctactttga ggatatcaat acctttctct 900
aaaatgtttt ggtgaagcca tctttaaagc taattgttca agattcaacc attgggacgt 960
ctcaaatgat tagatcctat aatactccta cgtactaaat tataagtcgt tttgatttta 1020
ttggtacata cattttgcta tgtgtttaga tataataata tgtctagata cattggatga 1080
accgaaaaaa tcgaaacgac ttataatttg gatcgaaagg agtatttgct aaagtccttt 1140
tcgaagttcc ggctctaaat ttttggataa aattttatga aatactatct taagaagtaa 1200
tttgactaga gaagcttgaa gagtataatc tcttaatttt gtgctacagg agtgaagcca 1260
acgtcgtatt tagatctaga tgctgtcagg tagtgaggac ggagggagta ttggataaag 1320
tcattccaag atcttagaaa attaaagtat attaagtttg attaaattta tatgacaagt 1380
aataacattc atgatgccaa ttaagtatca ttagattctt catcaactat attttcatag 1440
tatacttatt taatgttata aatttttata atttttttta taattttagc taaactcgag 1500
atcgattctt ataattaaaa ataaactgaa aaaaaatcac atgttcaagt gacaggagga 1560
gccagtttaa cggcgtcgac aagtctaacg gacaccaacc agcgaaccac cagcgccgag 1620
ccaatcccaa gcgaagccga ctgcagacgg ccgagacgtt gacacctttg gcgcggcatc 1680
catctctccg gccccctctt gagagttccg ccccaccggc ggcggtttcc aagtccgttc 1740
cgcccgcctt cgcggttgga cttgttccgg tggcgcctgg cggatcgcgt ggcggagcgg 1800
agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc tctcacacgg cacggaacgg 1860
aaccgtgacg gcaccgggca gcacgggcgg gattccttcc ccacctctcc ttcggtcctc 1920
cctccatcat aaatagccac ccccctccca ccttctttcc ccacctcgtc tcccctcgtg 1980
ttattcggag cacagacaca ccccgatccc caatcctctc ctcgcgagcc tcgtcgatcc 2040
ccgcttcaag gtacggcgat catcctccct ccctaactcc aatccgtggt tagggcctgc 2100
tagatcgtcc tccctcccta cctgcgatcc gtggttcgcg cctgctagtt ctgtttcctg 2160
tttgtcgatg gctgcgaggt ataatagatc tgatggcgtg cggtgtgacg gttaaattca 2220
catgctcttg cgatttatac gcgaatcgat ctgggattgc tcgagatcgg tgatccatgg 2280
ttagaaccct aggcggtgga gtcgggttaa atccgtgctg ttagggttcg taggtggatg 2340
cgacctgttc tggttgttta cttgtcagta tttaggaatc ctactaggat ggttctagct 2400
ggttcgcaga tgagatcgat ttcatgatct gctatatctt tcgttgccta agtttcgttt 2460
aatctgtccg tggtatgatg ttagcctttg atatgcttcg atcgtgctag ctacctcctg 2520
tgcactaaat tatcagctcg taatttttag catgcccttt tttttttggg tattgttcga 2580
ttgaggtgtc gttctagatc agagtaggaa gactgtttca aactacctgc tggatttatt 2640
aaatttggat ctgtatgagt atcacatata tctccataat ttagatggat ggaaatatcc 2700
ctttttcttt tagatactgt ttggtataga ttttgctgtg ggttttactg gtacttagat 2760
actcttcgtt tagatatgga tatgtttaca tgcagataca tgaagcaaca tgctgctaca 2820
gtttaatatg gataggtgta tatgttgttg tgggtccttt acttacatgc ttagatacat 2880
gaagcaacat gctgctacgt ttaataatta ttgtttatct gatctgattt aaacaaacat 2940
gctttttaat tgtcctgaaa tgcttggatg atggcatatg cagcagctat gtgtggattt 3000
taaataccca gcatgagcat gcatgaccct aacttagtat gctgtttatt tgcttgactt 3060
ttcttttgtt gatactcacc cttttgtttg ttgactcttg caggtg 3106
<210> 93
<211> 1964
<212> DNA
<213> Sorghastrum nutans
<400> 93
agctttgacc aacacatgag atgggcgcct tacgtttggc acaatgtaat gtagtccggc 60
acggcaagtt agactctgtg tgtagtgtta tattagccgg cctctttagg tttggcacaa 120
tttaattgaa tccggcatgg caagttagac tgcagtgtga gccggtcacc gcaagttagg 180
atataatata caagagcaag tatacaataa agtgacatta gcgtaaagtt atatgacata 240
tggaatataa gagaaaatac ggagtatata ataaggtgaa ctgtatagcg atcaaattta 300
tgctaagcga agaaaagaga agataaatag gttgaaaact tatagtgagc tttggctcat 360
aatctaaata attatgagag agtgggatcg accacatatt cattttgtag tacgtactct 420
ctccgttttt tataagttgc tttgattttt ttttatatca attttgctat acatctaaac 480
ataataggaa tatcaagttc atgaaggtcg tgatttgcac taaatatgtt cccttattag 540
atagacgagt tgtttagttt tattgtagat gatatagcgc ttgcatatag catgtgaacc 600
ggctaaatta ttagccatac acgactataa aaaatgacat tcctttgagg aacttttatg 660
caaccaaata gtcaacttca atgttgctag agcgggcttt aagccaaaag cagctgctgc 720
tttgtttccg agagaaggga cattctagtt gatagcaaaa caaatacgta gcagttgtag 780
cgagtgtgtg agtaataatt tttctctagt gtgtacgagt atgcgagtaa taattttaaa 840
tctctagaag gaagaaaaat aatattgcta cctactttga ggatatcaat acctttctct 900
aaaatgtttt ggtgaagcca tctttaaagc taattgttca agattcaacc attgggacgt 960
ctcaaatgat tagatcctat aatactccta cgtactaaat tataagtcgt tttgatttta 1020
ttggtacata cattttgcta tgtgtttaga tataataata tgtctagata cattggatga 1080
accgaaaaaa tcgaaacgac ttataatttg gatcgaaagg agtatttgct aaagtccttt 1140
tcgaagttcc ggctctaaat ttttggataa aattttatga aatactatct taagaagtaa 1200
tttgactaga gaagcttgaa gagtataatc tcttaatttt gtgctacagg agtgaagcca 1260
acgtcgtatt tagatctaga tgctgtcagg tagtgaggac ggagggagta ttggataaag 1320
tcattccaag atcttagaaa attaaagtat attaagtttg attaaattta tatgacaagt 1380
aataacattc atgatgccaa ttaagtatca ttagattctt catcaactat attttcatag 1440
tatacttatt taatgttata aatttttata atttttttta taattttagc taaactcgag 1500
atcgattctt ataattaaaa ataaactgaa aaaaaatcac atgttcaagt gacaggagga 1560
gccagtttaa cggcgtcgac aagtctaacg gacaccaacc agcgaaccac cagcgccgag 1620
ccaatcccaa gcgaagccga ctgcagacgg ccgagacgtt gacacctttg gcgcggcatc 1680
catctctccg gccccctctt gagagttccg ccccaccggc ggcggtttcc aagtccgttc 1740
cgcccgcctt cgcggttgga cttgttccgg tggcgcctgg cggatcgcgt ggcggagcgg 1800
agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc tctcacacgg cacggaacgg 1860
aaccgtgacg gcaccgggca gcacgggcgg gattccttcc ccacctctcc ttcggtcctc 1920
cctccatcat aaatagccac ccccctccca ccttctttcc ccac 1964
<210> 94
<211> 1056
<212> DNA
<213> Sorghastrum nutans
<400> 94
gtacggcgat catcctccct ccctaactcc aatccgtggt tagggcctgc tagatcgtcc 60
tccctcccta cctgcgatcc gtggttcgcg cctgctagtt ctgtttcctg tttgtcgatg 120
gctgcgaggt ataatagatc tgatggcgtg cggtgtgacg gttaaattca catgctcttg 180
cgatttatac gcgaatcgat ctgggattgc tcgagatcgg tgatccatgg ttagaaccct 240
aggcggtgga gtcgggttaa atccgtgctg ttagggttcg taggtggatg cgacctgttc 300
tggttgttta cttgtcagta tttaggaatc ctactaggat ggttctagct ggttcgcaga 360
tgagatcgat ttcatgatct gctatatctt tcgttgccta agtttcgttt aatctgtccg 420
tggtatgatg ttagcctttg atatgcttcg atcgtgctag ctacctcctg tgcactaaat 480
tatcagctcg taatttttag catgcccttt tttttttggg tattgttcga ttgaggtgtc 540
gttctagatc agagtaggaa gactgtttca aactacctgc tggatttatt aaatttggat 600
ctgtatgagt atcacatata tctccataat ttagatggat ggaaatatcc ctttttcttt 660
tagatactgt ttggtataga ttttgctgtg ggttttactg gtacttagat actcttcgtt 720
tagatatgga tatgtttaca tgcagataca tgaagcaaca tgctgctaca gtttaatatg 780
gataggtgta tatgttgttg tgggtccttt acttacatgc ttagatacat gaagcaacat 840
gctgctacgt ttaataatta ttgtttatct gatctgattt aaacaaacat gctttttaat 900
tgtcctgaaa tgcttggatg atggcatatg cagcagctat gtgtggattt taaataccca 960
gcatgagcat gcatgaccct aacttagtat gctgtttatt tgcttgactt ttcttttgtt 1020
gatactcacc cttttgtttg ttgactcttg caggtg 1056
<210> 95
<211> 2165
<212> DNA
<213> Sorghastrum nutans
<400> 95
gattcaacca ttgggacgtc tcaaatgatt agatcctata atactcctac gtactaaatt 60
ataagtcgtt ttgattttat tggtacatac attttgctat gtgtttagat ataataatat 120
gtctagatac attggatgaa ccgaaaaaat cgaaacgact tataatttgg atcgaaagga 180
gtatttgcta aagtcctttt cgaagttccg gctctaaatt tttggataaa attttatgaa 240
atactatctt aagaagtaat ttgactagag aagcttgaag agtataatct cttaattttg 300
tgctacagga gtgaagccaa cgtcgtattt agatctagat gctgtcaggt agtgaggacg 360
gagggagtat tggataaagt cattccaaga tcttagaaaa ttaaagtata ttaagtttga 420
ttaaatttat atgacaagta ataacattca tgatgccaat taagtatcat tagattcttc 480
atcaactata ttttcatagt atacttattt aatgttataa atttttataa ttttttttat 540
aattttagct aaactcgaga tcgattctta taattaaaaa taaactgaaa aaaaatcaca 600
tgttcaagtg acaggaggag ccagtttaac ggcgtcgaca agtctaacgg acaccaacca 660
gcgaaccacc agcgccgagc caatcccaag cgaagccgac tgcagacggc cgagacgttg 720
acacctttgg cgcggcatcc atctctccgg ccccctcttg agagttccgc cccaccggcg 780
gcggtttcca agtccgttcc gcccgccttc gcggttggac ttgttccggt ggcgcctggc 840
ggatcgcgtg gcggagcgga gacgacgagg tgagccgtgg gcgttcctcc tcctgctcct 900
ctcacacggc acggaacgga accgtgacgg caccgggcag cacgggcggg attccttccc 960
cacctctcct tcggtcctcc ctccatcata aatagccacc cccctcccac cttctttccc 1020
cacctcgtct cccctcgtgt tattcggagc acagacacac cccgatcccc aatcctctcc 1080
tcgcgagcct cgtcgatccc cgcttcaagg tacggcgatc atcctccctc cctaactcca 1140
atccgtggtt agggcctgct agatcgtcct ccctccctac ctgcgatccg tggttcgcgc 1200
ctgctagttc tgtttcctgt ttgtcgatgg ctgcgaggta taatagatct gatggcgtgc 1260
ggtgtgacgg ttaaattcac atgctcttgc gatttatacg cgaatcgatc tgggattgct 1320
cgagatcggt gatccatggt tagaacccta ggcggtggag tcgggttaaa tccgtgctgt 1380
tagggttcgt aggtggatgc gacctgttct ggttgtttac ttgtcagtat ttaggaatcc 1440
tactaggatg gttctagctg gttcgcagat gagatcgatt tcatgatctg ctatatcttt 1500
cgttgcctaa gtttcgttta atctgtccgt ggtatgatgt tagcctttga tatgcttcga 1560
tcgtgctagc tacctcctgt gcactaaatt atcagctcgt aatttttagc atgccctttt 1620
ttttttgggt attgttcgat tgaggtgtcg ttctagatca gagtaggaag actgtttcaa 1680
actacctgct ggatttatta aatttggatc tgtatgagta tcacatatat ctccataatt 1740
tagatggatg gaaatatccc tttttctttt agatactgtt tggtatagat tttgctgtgg 1800
gttttactgg tacttagata ctcttcgttt agatatggat atgtttacat gcagatacat 1860
gaagcaacat gctgctacag tttaatatgg ataggtgtat atgttgttgt gggtccttta 1920
cttacatgct tagatacatg aagcaacatg ctgctacgtt taataattat tgtttatctg 1980
atctgattta aacaaacatg ctttttaatt gtcctgaaat gcttggatga tggcatatgc 2040
agcagctatg tgtggatttt aaatacccag catgagcatg catgacccta acttagtatg 2100
ctgtttattt gcttgacttt tcttttgttg atactcaccc ttttgtttgt tgactcttgc 2160
aggtg 2165
<210> 96
<211> 1023
<212> DNA
<213> Sorghastrum nutans
<400> 96
gattcaacca ttgggacgtc tcaaatgatt agatcctata atactcctac gtactaaatt 60
ataagtcgtt ttgattttat tggtacatac attttgctat gtgtttagat ataataatat 120
gtctagatac attggatgaa ccgaaaaaat cgaaacgact tataatttgg atcgaaagga 180
gtatttgcta aagtcctttt cgaagttccg gctctaaatt tttggataaa attttatgaa 240
atactatctt aagaagtaat ttgactagag aagcttgaag agtataatct cttaattttg 300
tgctacagga gtgaagccaa cgtcgtattt agatctagat gctgtcaggt agtgaggacg 360
gagggagtat tggataaagt cattccaaga tcttagaaaa ttaaagtata ttaagtttga 420
ttaaatttat atgacaagta ataacattca tgatgccaat taagtatcat tagattcttc 480
atcaactata ttttcatagt atacttattt aatgttataa atttttataa ttttttttat 540
aattttagct aaactcgaga tcgattctta taattaaaaa taaactgaaa aaaaatcaca 600
tgttcaagtg acaggaggag ccagtttaac ggcgtcgaca agtctaacgg acaccaacca 660
gcgaaccacc agcgccgagc caatcccaag cgaagccgac tgcagacggc cgagacgttg 720
acacctttgg cgcggcatcc atctctccgg ccccctcttg agagttccgc cccaccggcg 780
gcggtttcca agtccgttcc gcccgccttc gcggttggac ttgttccggt ggcgcctggc 840
ggatcgcgtg gcggagcgga gacgacgagg tgagccgtgg gcgttcctcc tcctgctcct 900
ctcacacggc acggaacgga accgtgacgg caccgggcag cacgggcggg attccttccc 960
cacctctcct tcggtcctcc ctccatcata aatagccacc cccctcccac cttctttccc 1020
cac 1023
<210> 97
<211> 1866
<212> DNA
<213> Sorghastrum nutans
<400> 97
gtgctacagg agtgaagcca acgtcgtatt tagatctaga tgctgtcagg tagtgaggac 60
ggagggagta ttggataaag tcattccaag atcttagaaa attaaagtat attaagtttg 120
attaaattta tatgacaagt aataacattc atgatgccaa ttaagtatca ttagattctt 180
catcaactat attttcatag tatacttatt taatgttata aatttttata atttttttta 240
taattttagc taaactcgag atcgattctt ataattaaaa ataaactgaa aaaaaatcac 300
atgttcaagt gacaggagga gccagtttaa cggcgtcgac aagtctaacg gacaccaacc 360
agcgaaccac cagcgccgag ccaatcccaa gcgaagccga ctgcagacgg ccgagacgtt 420
gacacctttg gcgcggcatc catctctccg gccccctctt gagagttccg ccccaccggc 480
ggcggtttcc aagtccgttc cgcccgcctt cgcggttgga cttgttccgg tggcgcctgg 540
cggatcgcgt ggcggagcgg agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc 600
tctcacacgg cacggaacgg aaccgtgacg gcaccgggca gcacgggcgg gattccttcc 660
ccacctctcc ttcggtcctc cctccatcat aaatagccac ccccctccca ccttctttcc 720
ccacctcgtc tcccctcgtg ttattcggag cacagacaca ccccgatccc caatcctctc 780
ctcgcgagcc tcgtcgatcc ccgcttcaag gtacggcgat catcctccct ccctaactcc 840
aatccgtggt tagggcctgc tagatcgtcc tccctcccta cctgcgatcc gtggttcgcg 900
cctgctagtt ctgtttcctg tttgtcgatg gctgcgaggt ataatagatc tgatggcgtg 960
cggtgtgacg gttaaattca catgctcttg cgatttatac gcgaatcgat ctgggattgc 1020
tcgagatcgg tgatccatgg ttagaaccct aggcggtgga gtcgggttaa atccgtgctg 1080
ttagggttcg taggtggatg cgacctgttc tggttgttta cttgtcagta tttaggaatc 1140
ctactaggat ggttctagct ggttcgcaga tgagatcgat ttcatgatct gctatatctt 1200
tcgttgccta agtttcgttt aatctgtccg tggtatgatg ttagcctttg atatgcttcg 1260
atcgtgctag ctacctcctg tgcactaaat tatcagctcg taatttttag catgcccttt 1320
tttttttggg tattgttcga ttgaggtgtc gttctagatc agagtaggaa gactgtttca 1380
aactacctgc tggatttatt aaatttggat ctgtatgagt atcacatata tctccataat 1440
ttagatggat ggaaatatcc ctttttcttt tagatactgt ttggtataga ttttgctgtg 1500
ggttttactg gtacttagat actcttcgtt tagatatgga tatgtttaca tgcagataca 1560
tgaagcaaca tgctgctaca gtttaatatg gataggtgta tatgttgttg tgggtccttt 1620
acttacatgc ttagatacat gaagcaacat gctgctacgt ttaataatta ttgtttatct 1680
gatctgattt aaacaaacat gctttttaat tgtcctgaaa tgcttggatg atggcatatg 1740
cagcagctat gtgtggattt taaataccca gcatgagcat gcatgaccct aacttagtat 1800
gctgtttatt tgcttgactt ttcttttgtt gatactcacc cttttgtttg ttgactcttg 1860
caggtg 1866
<210> 98
<211> 724
<212> DNA
<213> Sorghastrum nutans
<400> 98
gtgctacagg agtgaagcca acgtcgtatt tagatctaga tgctgtcagg tagtgaggac 60
ggagggagta ttggataaag tcattccaag atcttagaaa attaaagtat attaagtttg 120
attaaattta tatgacaagt aataacattc atgatgccaa ttaagtatca ttagattctt 180
catcaactat attttcatag tatacttatt taatgttata aatttttata atttttttta 240
taattttagc taaactcgag atcgattctt ataattaaaa ataaactgaa aaaaaatcac 300
atgttcaagt gacaggagga gccagtttaa cggcgtcgac aagtctaacg gacaccaacc 360
agcgaaccac cagcgccgag ccaatcccaa gcgaagccga ctgcagacgg ccgagacgtt 420
gacacctttg gcgcggcatc catctctccg gccccctctt gagagttccg ccccaccggc 480
ggcggtttcc aagtccgttc cgcccgcctt cgcggttgga cttgttccgg tggcgcctgg 540
cggatcgcgt ggcggagcgg agacgacgag gtgagccgtg ggcgttcctc ctcctgctcc 600
tctcacacgg cacggaacgg aaccgtgacg gcaccgggca gcacgggcgg gattccttcc 660
ccacctctcc ttcggtcctc cctccatcat aaatagccac ccccctccca ccttctttcc 720
ccac 724
<210> 99
<211> 2625
<212> DNA
<213> Setaria italica
<400> 99
actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60
ggagcagcga tctggattgg agagaataga ggaaagagag ggaaaaggag agagatagcg 120
caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180
catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240
ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300
gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360
tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420
aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480
ttagaagctt ctactgcgct tttatattat agctgtggac ccgtggtaac ctttctcttt 540
tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600
cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660
ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720
cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc 780
atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840
gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900
ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960
aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020
acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg 1080
gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg 1140
tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt 1200
tggcggaaga aaggaatggc tcgtaggggc ccgggtagaa tcgaagaatg ttgcgctggg 1260
cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg 1320
gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg 1380
acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag 1440
caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cccgttgccg 1500
caagactcag atcagattcc gatccccagt tcttccccaa tcaccttgtg gtctctcgtg 1560
tcgcggttcc cagggacgcc tccggctcgt cgctcgacag cgatctccgc cccagcaagg 1620
tatagattca gttccttgct ccgatcccaa tctggttgag atgttgctcc gatgcgactt 1680
gattatgtca tatatctgcg gtttgcaccg atctgaagcc tagggtttct cgagcgaccc 1740
agttatttgc aatttgcgat ttgctcgttt gttgcgcagc gtagtttatg tttggagtaa 1800
tcgaggattt gtatgcggcg tcggcgctac ctgcttaatc acgccatgtg acgcggttac 1860
ttgcagaggc tgggttctgt tatgtcgtga tctaagaatc tagattaggc tcagtcgttc 1920
ttgctgtcga ctagtttgtt ttgatatcca tgtagtacaa gttacttaaa atttaggtcc 1980
aatatatttt gcatgctttt ggcctgttat tcttgccaac aagttgtcct ggtaaaaagt 2040
agatgtgaaa gtcacgtatt gggacaaatt gatggtttag tgctatagtt ctatagttct 2100
gtgatacatc tatctgattt tttttggtct attggtgcct aacttatctg aaaatcatgg 2160
aacatgaggc tagtttgatc atggtttagt tcattgtgat taataatgta tgatttagta 2220
gctattttgg tgatcgtgtc attttatttg tgaatggaat cattgtatgt aaatgaagct 2280
agttcagggg ttacgatgta gctggctttg tattctaaag gctgctatta ttcatccatc 2340
gatttcacct atatgtaatc cagagctttt gatgtgaaat ttgtctgatc cttcactagg 2400
aaggacagaa cattgttaat attttggcac atctgtctta ttctcatcct ttgtttgaac 2460
atgttagcct gttcaaacag atactgttgt aatgtcctag ttatataggt acatatgtgt 2520
tctctattga gtttatggac ttttgtgtgt gaagttatat ttcattttgc tcaaaactca 2580
tgtttgcaag ctttctgaca ttattctatt gttctgaaac agggt 2625
<210> 100
<211> 1492
<212> DNA
<213> Setaria italica
<400> 100
actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60
ggagcagcga tctggattgg agagaataga ggaaagagag ggaaaaggag agagatagcg 120
caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180
catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240
ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300
gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360
tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420
aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480
ttagaagctt ctactgcgct tttatattat agctgtggac ccgtggtaac ctttctcttt 540
tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600
cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660
ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720
cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc 780
atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840
gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900
ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960
aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020
acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg 1080
gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg 1140
tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt 1200
tggcggaaga aaggaatggc tcgtaggggc ccgggtagaa tcgaagaatg ttgcgctggg 1260
cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg 1320
gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg 1380
acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag 1440
caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cc 1492
<210> 101
<211> 127
<212> DNA
<213> Setaria italica
<400> 101
cgttgccgca agactcagat cagattccga tccccagttc ttccccaatc accttgtggt 60
ctctcgtgtc gcggttccca gggacgcctc cggctcgtcg ctcgacagcg atctccgccc 120
cagcaag 127
<210> 102
<211> 1006
<212> DNA
<213> Setaria italica
<400> 102
gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 60
tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 120
cagttatttg caatttgcga tttgctcgtt tgttgcgcag cgtagtttat gtttggagta 180
atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 240
cttgcagagg ctgggttctg ttatgtcgtg atctaagaat ctagattagg ctcagtcgtt 300
cttgctgtcg actagtttgt tttgatatcc atgtagtaca agttacttaa aatttaggtc 360
caatatattt tgcatgcttt tggcctgtta ttcttgccaa caagttgtcc tggtaaaaag 420
tagatgtgaa agtcacgtat tgggacaaat tgatggttta gtgctatagt tctatagttc 480
tgtgatacat ctatctgatt ttttttggtc tattggtgcc taacttatct gaaaatcatg 540
gaacatgagg ctagtttgat catggtttag ttcattgtga ttaataatgt atgatttagt 600
agctattttg gtgatcgtgt cattttattt gtgaatggaa tcattgtatg taaatgaagc 660
tagttcaggg gttacgatgt agctggcttt gtattctaaa ggctgctatt attcatccat 720
cgatttcacc tatatgtaat ccagagcttt tgatgtgaaa tttgtctgat ccttcactag 780
gaaggacaga acattgttaa tattttggca catctgtctt attctcatcc tttgtttgaa 840
catgttagcc tgttcaaaca gatactgttg taatgtccta gttatatagg tacatatgtg 900
ttctctattg agtttatgga cttttgtgtg tgaagttata tttcattttg ctcaaaactc 960
atgtttgcaa gctttctgac attattctat tgttctgaaa cagggt 1006
<210> 103
<211> 2625
<212> DNA
<213> Setaria italica
<400> 103
actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60
ggagcagcga tctggattgg agagaataga ggaaagagag ggaaaaggag agagatagcg 120
caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180
catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240
ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300
gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360
tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420
aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480
ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt 540
tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600
cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660
ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720
cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc 780
atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840
gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900
ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960
aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020
acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg 1080
gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg 1140
tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt 1200
tggcggaaga aaggaatggc tcgtaggggc ccgggtagaa tcgaagaatg ttgcgctggg 1260
cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg 1320
gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg 1380
acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag 1440
caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cccgttgccg 1500
caagactcag atcagattcc gatccccagt tcttccccaa tcaccttgtg gtctctcgtg 1560
tcgcggttcc cagggacgcc tccggctcgt cgctcgacag cgatctccgc cccagcaagg 1620
tatagattca gttccttgct ccgatcccaa tctggttgag atgttgctcc gatgcgactt 1680
gattatgtca tatatctgcg gtttgcaccg atctgaagcc tagggtttct cgagcgaccc 1740
agttatttgc aatttgcgat ttgctcgttt gttgcgcagc gtagtttatg tttggagtaa 1800
tcgaggattt gtatgcggcg tcggcgctac ctgcttaatc acgccatgtg acgcggttac 1860
ttgcagaggc tgggttctgt tatgtcgtga tctaagaatc tagattaggc tcagtcgttc 1920
ttgctgtcga ctagtttgtt ttgatatcca tgtagtacaa gttacttaaa atttaggtcc 1980
aatatatttt gcatgctttt ggcctgttat tcttgccaac aagttgtcct ggtaaaaagt 2040
agatgtgaaa gtcacgtatt gggacaaatt gatggtttag tgctatagtt ctatagttct 2100
gtgatacatc tatctgattt tttttggtct attggtgcct aacttatctg aaaatcatgg 2160
aacatgaggc tagtttgatc atggtttagt tcattgtgat taataatgta tgatttagta 2220
gctattttgg tgatcgtgtc attttatttg tgaatggaat cattgtatgt aaatgaagct 2280
agttcagggg ttacgatgta gctggctttg tattctaaag gctgctatta ttcatccatc 2340
gatttcacct atatgtaatc cagagctttt gatgtgaaat ttgtctgatc cttcactagg 2400
aaggacagaa cattgttaat attttggcac atctgtctta ttctcatcct ttgtttgaac 2460
atgttagcct gttcaaacag atactgttgt aatgtcctag ttatataggt acatatgtgt 2520
tctctattga gtttatggac ttttgtgtgt gaagttatat ttcattttgc tcaaaactca 2580
tgtttgcaag ctttctgaca ttattctatt gttctgaaac aggtg 2625
<210> 104
<211> 1492
<212> DNA
<213> Setaria italica
<400> 104
actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60
ggagcagcga tctggattgg agagaataga ggaaagagag ggaaaaggag agagatagcg 120
caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180
catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240
ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300
gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360
tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420
aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480
ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt 540
tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600
cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660
ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720
cttgtcataa tgccattacg tggattacac gtaactggcc ctgtaactac tcgttcggcc 780
atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840
gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900
ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960
aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020
acatccaacg gccaacagct cgacgtgccg gtcagcagag catcggaaca ctggtgattg 1080
gtggagccgg cagtatgcgc cccagcacgg ccgaggtggt ggtggcccgt ggccctgctg 1140
tctgcgcggc tcgggacaac ttgaaactgg gccaccgcct cgtcgcaact cgcaacccgt 1200
tggcggaaga aaggaatggc tcgtaggggc ccgggtagaa tcgaagaatg ttgcgctggg 1260
cttcgattca cataacatgg gcctgaagct ctaaaacgac ggcccggtcg ccgcgcgatg 1320
gaaagagacc ggatcctcct cgtgaattct ggaaggccac acgagagcga cccaccaccg 1380
acgcggagga gtcgtgcgtg gtccaacacg gccggcgggc tgggctgcga ccttaaccag 1440
caaggcacgc cacgacccgc cccgccctcg aggcataaat accctcccat cc 1492
<210> 105
<211> 1006
<212> DNA
<213> Setaria italica
<400> 105
gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 60
tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 120
cagttatttg caatttgcga tttgctcgtt tgttgcgcag cgtagtttat gtttggagta 180
atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 240
cttgcagagg ctgggttctg ttatgtcgtg atctaagaat ctagattagg ctcagtcgtt 300
cttgctgtcg actagtttgt tttgatatcc atgtagtaca agttacttaa aatttaggtc 360
caatatattt tgcatgcttt tggcctgtta ttcttgccaa caagttgtcc tggtaaaaag 420
tagatgtgaa agtcacgtat tgggacaaat tgatggttta gtgctatagt tctatagttc 480
tgtgatacat ctatctgatt ttttttggtc tattggtgcc taacttatct gaaaatcatg 540
gaacatgagg ctagtttgat catggtttag ttcattgtga ttaataatgt atgatttagt 600
agctattttg gtgatcgtgt cattttattt gtgaatggaa tcattgtatg taaatgaagc 660
tagttcaggg gttacgatgt agctggcttt gtattctaaa ggctgctatt attcatccat 720
cgatttcacc tatatgtaat ccagagcttt tgatgtgaaa tttgtctgat ccttcactag 780
gaaggacaga acattgttaa tattttggca catctgtctt attctcatcc tttgtttgaa 840
catgttagcc tgttcaaaca gatactgttg taatgtccta gttatatagg tacatatgtg 900
ttctctattg agtttatgga cttttgtgtg tgaagttata tttcattttg ctcaaaactc 960
atgtttgcaa gctttctgac attattctat tgttctgaaa caggtg 1006
<210> 106
<211> 2167
<212> DNA
<213> Setaria italica
<400> 106
gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg 60
acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt 120
aggcactagg cagagataga gccgggggtg aatggggcta aagctcagct gctcgagggg 180
ccgtgggctg gtttccacta gcctacagct gtgccacgtg cggccgcgca agccgaagca 240
agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac acgtaactgg 300
ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacggg 360
taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt 420
cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac 480
ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca 540
agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag 600
agcatcggaa cactggtgat tggtggagcc ggcagtatgc gccccagcac ggccgaggtg 660
gtggtggccc gtggccctgc tgtctgcgcg gctcgggaca acttgaaact gggccaccgc 720
ctcgtcgcaa ctcgcaaccc gttggcggaa gaaaggaatg gctcgtaggg gcccgggtag 780
aatcgaagaa tgttgcgctg ggcttcgatt cacataacat gggcctgaag ctctaaaacg 840
acggcccggt cgccgcgcga tggaaagaga ccggatcctc ctcgtgaatt ctggaaggcc 900
acacgagagc gacccaccac cgacgcggag gagtcgtgcg tggtccaaca cggccggcgg 960
gctgggctgc gaccttaacc agcaaggcac gccacgaccc gccccgccct cgaggcataa 1020
ataccctccc atcccgttgc cgcaagactc agatcagatt ccgatcccca gttcttcccc 1080
aatcaccttg tggtctctcg tgtcgcggtt cccagggacg cctccggctc gtcgctcgac 1140
agcgatctcc gccccagcaa ggtatagatt cagttccttg ctccgatccc aatctggttg 1200
agatgttgct ccgatgcgac ttgattatgt catatatctg cggtttgcac cgatctgaag 1260
cctagggttt ctcgagcgac ccagttattt gcaatttgcg atttgctcgt ttgttgcgca 1320
gcgtagttta tgtttggagt aatcgaggat ttgtatgcgg cgtcggcgct acctgcttaa 1380
tcacgccatg tgacgcggtt acttgcagag gctgggttct gttatgtcgt gatctaagaa 1440
tctagattag gctcagtcgt tcttgctgtc gactagtttg ttttgatatc catgtagtac 1500
aagttactta aaatttaggt ccaatatatt ttgcatgctt ttggcctgtt attcttgcca 1560
acaagttgtc ctggtaaaaa gtagatgtga aagtcacgta ttgggacaaa ttgatggttt 1620
agtgctatag ttctatagtt ctgtgataca tctatctgat tttttttggt ctattggtgc 1680
ctaacttatc tgaaaatcat ggaacatgag gctagtttga tcatggttta gttcattgtg 1740
attaataatg tatgatttag tagctatttt ggtgatcgtg tcattttatt tgtgaatgga 1800
atcattgtat gtaaatgaag ctagttcagg ggttacgatg tagctggctt tgtattctaa 1860
aggctgctat tattcatcca tcgatttcac ctatatgtaa tccagagctt ttgatgtgaa 1920
atttgtctga tccttcacta ggaaggacag aacattgtta atattttggc acatctgtct 1980
tattctcatc ctttgtttga acatgttagc ctgttcaaac agatactgtt gtaatgtcct 2040
agttatatag gtacatatgt gttctctatt gagtttatgg acttttgtgt gtgaagttat 2100
atttcatttt gctcaaaact catgtttgca agctttctga cattattcta ttgttctgaa 2160
acaggtg 2167
<210> 107
<211> 1034
<212> DNA
<213> Setaria italica
<400> 107
gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg 60
acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt 120
aggcactagg cagagataga gccgggggtg aatggggcta aagctcagct gctcgagggg 180
ccgtgggctg gtttccacta gcctacagct gtgccacgtg cggccgcgca agccgaagca 240
agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac acgtaactgg 300
ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacggg 360
taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt 420
cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac 480
ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca 540
agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag 600
agcatcggaa cactggtgat tggtggagcc ggcagtatgc gccccagcac ggccgaggtg 660
gtggtggccc gtggccctgc tgtctgcgcg gctcgggaca acttgaaact gggccaccgc 720
ctcgtcgcaa ctcgcaaccc gttggcggaa gaaaggaatg gctcgtaggg gcccgggtag 780
aatcgaagaa tgttgcgctg ggcttcgatt cacataacat gggcctgaag ctctaaaacg 840
acggcccggt cgccgcgcga tggaaagaga ccggatcctc ctcgtgaatt ctggaaggcc 900
acacgagagc gacccaccac cgacgcggag gagtcgtgcg tggtccaaca cggccggcgg 960
gctgggctgc gaccttaacc agcaaggcac gccacgaccc gccccgccct cgaggcataa 1020
ataccctccc atcc 1034
<210> 108
<211> 1813
<212> DNA
<213> Setaria italica
<400> 108
cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60
gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120
atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180
atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240
cagcagagca tcggaacact ggtgattggt ggagccggca gtatgcgccc cagcacggcc 300
gaggtggtgg tggcccgtgg ccctgctgtc tgcgcggctc gggacaactt gaaactgggc 360
caccgcctcg tcgcaactcg caacccgttg gcggaagaaa ggaatggctc gtaggggccc 420
gggtagaatc gaagaatgtt gcgctgggct tcgattcaca taacatgggc ctgaagctct 480
aaaacgacgg cccggtcgcc gcgcgatgga aagagaccgg atcctcctcg tgaattctgg 540
aaggccacac gagagcgacc caccaccgac gcggaggagt cgtgcgtggt ccaacacggc 600
cggcgggctg ggctgcgacc ttaaccagca aggcacgcca cgacccgccc cgccctcgag 660
gcataaatac cctcccatcc cgttgccgca agactcagat cagattccga tccccagttc 720
ttccccaatc accttgtggt ctctcgtgtc gcggttccca gggacgcctc cggctcgtcg 780
ctcgacagcg atctccgccc cagcaaggta tagattcagt tccttgctcc gatcccaatc 840
tggttgagat gttgctccga tgcgacttga ttatgtcata tatctgcggt ttgcaccgat 900
ctgaagccta gggtttctcg agcgacccag ttatttgcaa tttgcgattt gctcgtttgt 960
tgcgcagcgt agtttatgtt tggagtaatc gaggatttgt atgcggcgtc ggcgctacct 1020
gcttaatcac gccatgtgac gcggttactt gcagaggctg ggttctgtta tgtcgtgatc 1080
taagaatcta gattaggctc agtcgttctt gctgtcgact agtttgtttt gatatccatg 1140
tagtacaagt tacttaaaat ttaggtccaa tatattttgc atgcttttgg cctgttattc 1200
ttgccaacaa gttgtcctgg taaaaagtag atgtgaaagt cacgtattgg gacaaattga 1260
tggtttagtg ctatagttct atagttctgt gatacatcta tctgattttt tttggtctat 1320
tggtgcctaa cttatctgaa aatcatggaa catgaggcta gtttgatcat ggtttagttc 1380
attgtgatta ataatgtatg atttagtagc tattttggtg atcgtgtcat tttatttgtg 1440
aatggaatca ttgtatgtaa atgaagctag ttcaggggtt acgatgtagc tggctttgta 1500
ttctaaaggc tgctattatt catccatcga tttcacctat atgtaatcca gagcttttga 1560
tgtgaaattt gtctgatcct tcactaggaa ggacagaaca ttgttaatat tttggcacat 1620
ctgtcttatt ctcatccttt gtttgaacat gttagcctgt tcaaacagat actgttgtaa 1680
tgtcctagtt atataggtac atatgtgttc tctattgagt ttatggactt ttgtgtgtga 1740
agttatattt cattttgctc aaaactcatg tttgcaagct ttctgacatt attctattgt 1800
tctgaaacag gtg 1813
<210> 109
<211> 680
<212> DNA
<213> Setaria italica
<400> 109
cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60
gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120
atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180
atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240
cagcagagca tcggaacact ggtgattggt ggagccggca gtatgcgccc cagcacggcc 300
gaggtggtgg tggcccgtgg ccctgctgtc tgcgcggctc gggacaactt gaaactgggc 360
caccgcctcg tcgcaactcg caacccgttg gcggaagaaa ggaatggctc gtaggggccc 420
gggtagaatc gaagaatgtt gcgctgggct tcgattcaca taacatgggc ctgaagctct 480
aaaacgacgg cccggtcgcc gcgcgatgga aagagaccgg atcctcctcg tgaattctgg 540
aaggccacac gagagcgacc caccaccgac gcggaggagt cgtgcgtggt ccaacacggc 600
cggcgggctg ggctgcgacc ttaaccagca aggcacgcca cgacccgccc cgccctcgag 660
gcataaatac cctcccatcc 680
<210> 110
<211> 2634
<212> DNA
<213> Setaria viridis
<400> 110
actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60
ggagcagcga tctggattgg agagaataga ggaaagagag ggaaaaggag agagatagcg 120
caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180
catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240
ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300
gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360
tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420
aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480
ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt 540
tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600
cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660
ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720
cttgtcataa tgccattacg tggattacag gtaactggcc ctgtaactac tcgttcggcc 780
atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840
gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900
ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960
aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020
acatccaacg gccaacagct cgacgtgccg gtcagcagag acatcggaac actggtgatt 1080
ggtggagccg gcagtatgcg ccccagcacg gccgaggtgg tggtggcccg tggccctgct 1140
gtctgcgcgg ctcgggacaa cttgaaactg ggccaccgcc tcgtcgcaac tcgcaacccg 1200
ttggcggaag aaaggaatgg ctcgtagggg cccgggtaga atccaagaat gttgcgctgg 1260
gcttcgattc acataacatg ggcctgaagc tctaaaacga cggcccggtc accgggcgat 1320
ggaaagagac cggatcctcc tcgtgaattc tggaaggcca cacgagagcg acccaccacc 1380
gacgcggagg agtcgtgcgt ggtccaacac ggccggcggg ctgggctgcg accttaacca 1440
gcaaggcacg ccacgacccg cctcgccctc gaggcataaa taccctccca tcccgttgcc 1500
gcaagactca gatcagattc cgatccccag ttcttcccca atcaccttgt ggtctctcgt 1560
gtcgcggttc ccagggacgc ctccggctcg tcgctcgaca gcgatctccg ccccagcaag 1620
gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 1680
tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 1740
cagttgtttg caatttgcga tttgctcgtt tgttgcgcat cgtagtttat gtttggagta 1800
atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 1860
cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct 1920
cagtcgttct tgctgtcgac tagtttgttt tgatatccat gtagtacaag ttacttaaaa 1980
tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg 2040
gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc 2100
tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga 2160
aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat 2220
gatttagtag ctattttggt gatcgtgtca ttttatttgt gaatggaatc attgtatgta 2280
aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat 2340
tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc 2400
ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt 2460
tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta 2520
catatgtgtt ctctattgag tttatggact tttgtgtgtg aagttatatt tcattttgct 2580
caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca ggtg 2634
<210> 111
<211> 1493
<212> DNA
<213> Setaria viridis
<400> 111
actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60
ggagcagcga tctggattgg agagaataga ggaaagagag ggaaaaggag agagatagcg 120
caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180
catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240
ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300
gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360
tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420
aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480
ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt 540
tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600
cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660
ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720
cttgtcataa tgccattacg tggattacag gtaactggcc ctgtaactac tcgttcggcc 780
atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840
gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900
ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960
aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020
acatccaacg gccaacagct cgacgtgccg gtcagcagag acatcggaac actggtgatt 1080
ggtggagccg gcagtatgcg ccccagcacg gccgaggtgg tggtggcccg tggccctgct 1140
gtctgcgcgg ctcgggacaa cttgaaactg ggccaccgcc tcgtcgcaac tcgcaacccg 1200
ttggcggaag aaaggaatgg ctcgtagggg cccgggtaga atccaagaat gttgcgctgg 1260
gcttcgattc acataacatg ggcctgaagc tctaaaacga cggcccggtc accgggcgat 1320
ggaaagagac cggatcctcc tcgtgaattc tggaaggcca cacgagagcg acccaccacc 1380
gacgcggagg agtcgtgcgt ggtccaacac ggccggcggg ctgggctgcg accttaacca 1440
gcaaggcacg ccacgacccg cctcgccctc gaggcataaa taccctccca tcc 1493
<210> 112
<211> 127
<212> DNA
<213> Setaria viridis
<400> 112
cgttgccgca agactcagat cagattccga tccccagttc ttccccaatc accttgtggt 60
ctctcgtgtc gcggttccca gggacgcctc cggctcgtcg ctcgacagcg atctccgccc 120
cagcaag 127
<210> 113
<211> 1014
<212> DNA
<213> Setaria viridis
<400> 113
gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 60
tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 120
cagttgtttg caatttgcga tttgctcgtt tgttgcgcat cgtagtttat gtttggagta 180
atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 240
cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct 300
cagtcgttct tgctgtcgac tagtttgttt tgatatccat gtagtacaag ttacttaaaa 360
tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg 420
gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc 480
tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga 540
aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat 600
gatttagtag ctattttggt gatcgtgtca ttttatttgt gaatggaatc attgtatgta 660
aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat 720
tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc 780
ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt 840
tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta 900
catatgtgtt ctctattgag tttatggact tttgtgtgtg aagttatatt tcattttgct 960
caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca ggtg 1014
<210> 114
<211> 2634
<212> DNA
<213> Setaria viridis
<400> 114
actgccgcga cacgcctcac tggcgggagg gctccgagcg ctctctcccc ggcggccggc 60
ggagcagcga tctggattgg agagaataga ggaaagagag ggaaaaggag agagatagcg 120
caaagagctg aaaagataag gttgtgcggg ctgtggtgat tagaggacca ctaatccctc 180
catctcctaa tgacgcggtg cccaagacca gtgccgcggc acaccagcgt ctaagtgaac 240
ttccgctaac cttccggtca ttgcgcctga aagatgtcat gtggcgaggc ccccctctca 300
gtagattgcc aactgcctac cgtgccactc ttccatgcat gattgctccc gtctatcccg 360
tttctcacaa cagatagaca acagtaagca tcactaaagc aagcatgtgt agaaccttaa 420
aaaaaggctt atactaccag tatactatca accagcatgc cgtttttgaa gtatccagga 480
ttagaagctt ctactgcgct tttatattat agctgtggac ctgtggtaac ctttctcttt 540
tggcgcttgc ttaatctcgg ccgtgctggt ccatgcttag gcactaggca gagatagagc 600
cgggggtgaa tggggctaaa gctcagctgc tcgaggggcc gtgggctggt ttccactagc 660
ctacagctgt gccacgtgcg gccgcgcaag ccgaagcaag cacgctgagc cgttggacag 720
cttgtcataa tgccattacg tggattacag gtaactggcc ctgtaactac tcgttcggcc 780
atcatcaaac gacgacgtcc gctaggcgac gacacgggta atgcacgcag ccacccaggc 840
gcgcgcgcta gcggagcacg gtcaggtgac acgggcgtcg tgacgcttcc gagttgaagg 900
ggttaacgcc agaaacagtg tttggccagg gtatgaacat aacaaaaaat attcacacga 960
aagaatggaa gtatggagct gctactgtgt aaatgccaag caggaaactc acgcccgcta 1020
acatccaacg gccaacagct cgacgtgccg gtcagcagag acatcggaac actggtgatt 1080
ggtggagccg gcagtatgcg ccccagcacg gccgaggtgg tggtggcccg tggccctgct 1140
gtctgcgcgg ctcgggacaa cttgaaactg ggccaccgcc tcgtcgcaac tcgcaacccg 1200
ttggcggaag aaaggaatgg ctcgtagggg cccgggtaga atccaagaat gttgcgctgg 1260
gcttcgattc acataacatg ggcctgaagc tctaaaacga cggcccggtc accgggcgat 1320
ggaaagagac cggatcctcc tcgtgaattc tggaaggcca cacgagagcg acccaccacc 1380
gacgcggagg agtcgtgcgt ggtccaacac ggccggcggg ctgggctgcg accttaacca 1440
gcaaggcacg ccacgacccg cctcgccctc gaggcataaa taccctccca tcccgttgcc 1500
gcaagactca gatcagattc cgatccccag ttcttcccca atcaccttgt ggtctctcgt 1560
gtcgcggttc ccagggacgc ctccggctcg tcgctcgaca gcgatctccg ccccagcaag 1620
gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 1680
tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 1740
cagttgtttg caatttgcga tttgctcgtt tgttgcgcat cgtagtttat gtttggagta 1800
atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 1860
cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct 1920
cagtcgttct tgctgtcgac tagtttgttt tgatatccat gtagtacaag ttacttaaaa 1980
tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg 2040
gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc 2100
tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga 2160
aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat 2220
gatttagtag ctattttggt gatcgtgtca ttttatttgt gaatggaatc attgtatgta 2280
aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat 2340
tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc 2400
ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt 2460
tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta 2520
catatgtgtt ctctattgag tttatggact tttgtgtgtg aagttatatt tcattttgct 2580
caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca gggt 2634
<210> 115
<211> 1014
<212> DNA
<213> Setaria viridis
<400> 115
gtatagattc agttccttgc tccgatccca atctggttga gatgttgctc cgatgcgact 60
tgattatgtc atatatctgc ggtttgcacc gatctgaagc ctagggtttc tcgagcgacc 120
cagttgtttg caatttgcga tttgctcgtt tgttgcgcat cgtagtttat gtttggagta 180
atcgaggatt tgtatgcggc gtcggcgcta cctgcttaat cacgccatgt gacgcggtta 240
cttgcagagg ctgggttagt gggttctgtt atgtcgtgat ctaagaatct agattaggct 300
cagtcgttct tgctgtcgac tagtttgttt tgatatccat gtagtacaag ttacttaaaa 360
tttaggtcca atatattttg catgcttttg gcctgttatt cttgccaaca agttgtcctg 420
gtaaaaagta gatgtgaaag tcacgtattg ggacaaattg atggttaagt gctatagttc 480
tatagttctg tgatacatct atctgatttt ttttggtcta ttggtgccta acttatctga 540
aaatcatgga acatgaggct agtttgatca tggtttagtt cattgtgatt aataatgtat 600
gatttagtag ctattttggt gatcgtgtca ttttatttgt gaatggaatc attgtatgta 660
aatgaagcta gttcaggggt tatgatgtag ctggctttgt attctaaagg ctgctattat 720
tcatccatcg atttcaccta tatgtaatcc agagctttcg atgtgaaatt tgtctgatcc 780
ttcactagga aggacagaac attgttaata ttttggcaca tctgtcttat tctcatcctt 840
tgtttgaaca tgttagcctg ttcaaacaga tactgttgta atgtcctagt tatataggta 900
catatgtgtt ctctattgag tttatggact tttgtgtgtg aagttatatt tcattttgct 960
caaaactcat gtttgcaagc tttctgacat tattctattg ttctgaaaca gggt 1014
<210> 116
<211> 2176
<212> DNA
<213> Setaria viridis
<400> 116
gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg 60
acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt 120
aggcactagg cagagataga gccgggggtg aatggggcta aagctcagct gctcgagggg 180
ccgtgggctg gtttccacta gcctacagct gtgccacgtg cggccgcgca agccgaagca 240
agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac aggtaactgg 300
ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacggg 360
taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt 420
cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac 480
ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca 540
agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag 600
agacatcgga acactggtga ttggtggagc cggcagtatg cgccccagca cggccgaggt 660
ggtggtggcc cgtggccctg ctgtctgcgc ggctcgggac aacttgaaac tgggccaccg 720
cctcgtcgca actcgcaacc cgttggcgga agaaaggaat ggctcgtagg ggcccgggta 780
gaatccaaga atgttgcgct gggcttcgat tcacataaca tgggcctgaa gctctaaaac 840
gacggcccgg tcaccgggcg atggaaagag accggatcct cctcgtgaat tctggaaggc 900
cacacgagag cgacccacca ccgacgcgga ggagtcgtgc gtggtccaac acggccggcg 960
ggctgggctg cgaccttaac cagcaaggca cgccacgacc cgcctcgccc tcgaggcata 1020
aataccctcc catcccgttg ccgcaagact cagatcagat tccgatcccc agttcttccc 1080
caatcacctt gtggtctctc gtgtcgcggt tcccagggac gcctccggct cgtcgctcga 1140
cagcgatctc cgccccagca aggtatagat tcagttcctt gctccgatcc caatctggtt 1200
gagatgttgc tccgatgcga cttgattatg tcatatatct gcggtttgca ccgatctgaa 1260
gcctagggtt tctcgagcga cccagttgtt tgcaatttgc gatttgctcg tttgttgcgc 1320
atcgtagttt atgtttggag taatcgagga tttgtatgcg gcgtcggcgc tacctgctta 1380
atcacgccat gtgacgcggt tacttgcaga ggctgggtta gtgggttctg ttatgtcgtg 1440
atctaagaat ctagattagg ctcagtcgtt cttgctgtcg actagtttgt tttgatatcc 1500
atgtagtaca agttacttaa aatttaggtc caatatattt tgcatgcttt tggcctgtta 1560
ttcttgccaa caagttgtcc tggtaaaaag tagatgtgaa agtcacgtat tgggacaaat 1620
tgatggttaa gtgctatagt tctatagttc tgtgatacat ctatctgatt ttttttggtc 1680
tattggtgcc taacttatct gaaaatcatg gaacatgagg ctagtttgat catggtttag 1740
ttcattgtga ttaataatgt atgatttagt agctattttg gtgatcgtgt cattttattt 1800
gtgaatggaa tcattgtatg taaatgaagc tagttcaggg gttatgatgt agctggcttt 1860
gtattctaaa ggctgctatt attcatccat cgatttcacc tatatgtaat ccagagcttt 1920
cgatgtgaaa tttgtctgat ccttcactag gaaggacaga acattgttaa tattttggca 1980
catctgtctt attctcatcc tttgtttgaa catgttagcc tgttcaaaca gatactgttg 2040
taatgtccta gttatatagg tacatatgtg ttctctattg agtttatgga cttttgtgtg 2100
tgaagttata tttcattttg ctcaaaactc atgtttgcaa gctttctgac attattctat 2160
tgttctgaaa caggtg 2176
<210> 117
<211> 1035
<212> DNA
<213> Setaria viridis
<400> 117
gccgtttttg aagtatccag gattagaagc ttctactgcg cttttatatt atagctgtgg 60
acctgtggta acctttctct tttggcgctt gcttaatctc ggccgtgctg gtccatgctt 120
aggcactagg cagagataga gccgggggtg aatggggcta aagctcagct gctcgagggg 180
ccgtgggctg gtttccacta gcctacagct gtgccacgtg cggccgcgca agccgaagca 240
agcacgctga gccgttggac agcttgtcat aatgccatta cgtggattac aggtaactgg 300
ccctgtaact actcgttcgg ccatcatcaa acgacgacgt ccgctaggcg acgacacggg 360
taatgcacgc agccacccag gcgcgcgcgc tagcggagca cggtcaggtg acacgggcgt 420
cgtgacgctt ccgagttgaa ggggttaacg ccagaaacag tgtttggcca gggtatgaac 480
ataacaaaaa atattcacac gaaagaatgg aagtatggag ctgctactgt gtaaatgcca 540
agcaggaaac tcacgcccgc taacatccaa cggccaacag ctcgacgtgc cggtcagcag 600
agacatcgga acactggtga ttggtggagc cggcagtatg cgccccagca cggccgaggt 660
ggtggtggcc cgtggccctg ctgtctgcgc ggctcgggac aacttgaaac tgggccaccg 720
cctcgtcgca actcgcaacc cgttggcgga agaaaggaat ggctcgtagg ggcccgggta 780
gaatccaaga atgttgcgct gggcttcgat tcacataaca tgggcctgaa gctctaaaac 840
gacggcccgg tcaccgggcg atggaaagag accggatcct cctcgtgaat tctggaaggc 900
cacacgagag cgacccacca ccgacgcgga ggagtcgtgc gtggtccaac acggccggcg 960
ggctgggctg cgaccttaac cagcaaggca cgccacgacc cgcctcgccc tcgaggcata 1020
aataccctcc catcc 1035
<210> 118
<211> 1822
<212> DNA
<213> Setaria viridis
<400> 118
cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60
gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120
atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180
atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240
cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc 300
cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg 360
ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtaggggcc 420
cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc 480
taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctc gtgaattctg 540
gaaggccaca cgagagcgac ccaccaccga cgcggaggag tcgtgcgtgg tccaacacgg 600
ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga 660
ggcataaata ccctcccatc ccgttgccgc aagactcaga tcagattccg atccccagtt 720
cttccccaat caccttgtgg tctctcgtgt cgcggttccc agggacgcct ccggctcgtc 780
gctcgacagc gatctccgcc ccagcaaggt atagattcag ttccttgctc cgatcccaat 840
ctggttgaga tgttgctccg atgcgacttg attatgtcat atatctgcgg tttgcaccga 900
tctgaagcct agggtttctc gagcgaccca gttgtttgca atttgcgatt tgctcgtttg 960
ttgcgcatcg tagtttatgt ttggagtaat cgaggatttg tatgcggcgt cggcgctacc 1020
tgcttaatca cgccatgtga cgcggttact tgcagaggct gggttagtgg gttctgttat 1080
gtcgtgatct aagaatctag attaggctca gtcgttcttg ctgtcgacta gtttgttttg 1140
atatccatgt agtacaagtt acttaaaatt taggtccaat atattttgca tgcttttggc 1200
ctgttattct tgccaacaag ttgtcctggt aaaaagtaga tgtgaaagtc acgtattggg 1260
acaaattgat ggttaagtgc tatagttcta tagttctgtg atacatctat ctgatttttt 1320
ttggtctatt ggtgcctaac ttatctgaaa atcatggaac atgaggctag tttgatcatg 1380
gtttagttca ttgtgattaa taatgtatga tttagtagct attttggtga tcgtgtcatt 1440
ttatttgtga atggaatcat tgtatgtaaa tgaagctagt tcaggggtta tgatgtagct 1500
ggctttgtat tctaaaggct gctattattc atccatcgat ttcacctata tgtaatccag 1560
agctttcgat gtgaaatttg tctgatcctt cactaggaag gacagaacat tgttaatatt 1620
ttggcacatc tgtcttattc tcatcctttg tttgaacatg ttagcctgtt caaacagata 1680
ctgttgtaat gtcctagtta tataggtaca tatgtgttct ctattgagtt tatggacttt 1740
tgtgtgtgaa gttatatttc attttgctca aaactcatgt ttgcaagctt tctgacatta 1800
ttctattgtt ctgaaacagg tg 1822
<210> 119
<211> 681
<212> DNA
<213> Setaria viridis
<400> 119
cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60
gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120
atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180
atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240
cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc 300
cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg 360
ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtaggggcc 420
cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc 480
taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctc gtgaattctg 540
gaaggccaca cgagagcgac ccaccaccga cgcggaggag tcgtgcgtgg tccaacacgg 600
ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga 660
ggcataaata ccctcccatc c 681
<210> 120
<211> 1822
<212> DNA
<213> Setaria viridis
<400> 120
cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60
gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120
atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180
atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240
cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc 300
cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg 360
ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtaggggcc 420
cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc 480
taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctt gtgaattctg 540
gaaggccaca cgagagcgac ccaccaccga cgcggaggag tcgtgcgtgg tccaacacgg 600
ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga 660
ggcataaata ccctcccatc ccgttgccgc aagactcaga tcagattccg atccccagtt 720
cttccccaat caccttgtgg tctctcgtgt cgcggttccc agggacgcct ccggctcgtc 780
gctcgacagc gatctccgcc ccagcaaggt atagattcag ttccttgctc cgatcccaat 840
ctggttgaga tgttgctccg atgcgacttg attatgtcat atatctgcgg tttgcaccga 900
tctgaagcct agggtttctc gagcgaccca gttgtttgca atttgcgatt tgctcgtttg 960
ttgcgcatcg tagtttatgt ttggagtaat cgaggatttg tatgcggcgt cggcgctacc 1020
tgcttaatca cgccatgtga cgcggttact tgcagaggct gggttagtgg gttctgttat 1080
gtcgtgatct aagaatctag attaggctca gtcgttcttg ctgtcgacta gtttgttttg 1140
atatccatgt agtacaagtt acttaaaatt taggtccaat atattttgca tgcttttggc 1200
ctgttattct tgccaacaag ttgtcctggt aaaaagtaga tgtgaaagtc acgtattggg 1260
acaaattgat ggttaagtgc tatagttcta tagttctgtg atacatctat ctgatttttt 1320
ttggtctatt ggtgcctaac ttatctgaaa atcatggaac atgaggctag tttgatcatg 1380
gtttagttca ttgtgattaa taatgtatga tttagtagct attttggtga tcgtgtcatt 1440
ttatttgtga atggaatcat tgtatgtaaa tgaagctagt tcaggggtta tgatgtagct 1500
ggctttgtat tctaaaggct gctattattc atccatcgat ttcacctata tgtaatccag 1560
agctttcgat gtgaaatttg tctgatcctt cactaggaag gacagaacat tgttaatatt 1620
ttggcacatc tgtcttattc tcatcctttg tttgaacatg ttagcctgtt caaacagata 1680
ctgttgtaat gtcctagtta tataggtaca tatgtgttct ctattgagtt tatggacttt 1740
tgtgtgtgaa gttatatttc attttgctca aaactcatgt ttgcaagctt tctgacatta 1800
ttctattgtt ctgaaacagg gt 1822
<210> 121
<211> 681
<212> DNA
<213> Setaria viridis
<400> 121
cacgggtaat gcacgcagcc acccaggcgc gcgcgctagc ggagcacggt caggtgacac 60
gggcgtcgtg acgcttccga gttgaagggg ttaacgccag aaacagtgtt tggccagggt 120
atgaacataa caaaaaatat tcacacgaaa gaatggaagt atggagctgc tactgtgtaa 180
atgccaagca ggaaactcac gcccgctaac atccaacggc caacagctcg acgtgccggt 240
cagcagagac atcggaacac tggtgattgg tggagccggc agtatgcgcc ccagcacggc 300
cgaggtggtg gtggcccgtg gccctgctgt ctgcgcggct cgggacaact tgaaactggg 360
ccaccgcctc gtcgcaactc gcaacccgtt ggcggaagaa aggaatggct cgtaggggcc 420
cgggtagaat ccaagaatgt tgcgctgggc ttcgattcac ataacatggg cctgaagctc 480
taaaacgacg gcccggtcac cgggcgatgg aaagagaccg gatcctcctt gtgaattctg 540
gaaggccaca cgagagcgac ccaccaccga cgcggaggag tcgtgcgtgg tccaacacgg 600
ccggcgggct gggctgcgac cttaaccagc aaggcacgcc acgacccgcc tcgccctcga 660
ggcataaata ccctcccatc c 681
<210> 122
<211> 1925
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 122
gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca 60
tatttttttt tgtcacactt gtgtttgaag tgcagtttat ctatctctat acatatattt 120
aaacttcact atatgaataa tatagtctat agtattaaaa taatatcaat gttttagatg 180
attatataac tgaactgcta gacatggtct aaaggacaac cgagtatttt gacaacatga 240
ctctacagtt ttatcttttt agtgtgcatg tgttcttttt acttttgcaa atagcttcac 300
ctatataata cttcatccat tttattagta catccattta ctaaattttt agtacatcta 360
ttttattcta ttttagcctc taaattaaga aaacttaaac tctattttag ttttttattt 420
aataatttag atataaaata gaataaaata aagtgactaa aaaataacta aatacctttt 480
aagaaataaa aaaactaagg aaccattttt cttgttccga gtagataatg acagcctgtt 540
caacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc 600
aagcgaagca gacggcacgg catctctgta gctgcctctg gacccctctc gagagttccg 660
ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac 720
gtgagccggc acggcaggcg gcctcctctc acggcaccgg cagctacggg ggattccttt 780
cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagaccc cctccacacc 840
ctctttcccc aacctcgtgt tcgttcggag cgcgcacaca cacaaccaga tctcccccaa 900
atccacccgt cggcacctcc gcttcaaggt acgccgctca tcctcctccc ccccctctct 960
ctaccttctc tagatcggcg tttcggtcca tggttagggc ccggtagttc tacttctgtt 1020
catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagatttc gtacacggat 1080
gcgacctgta catcagacat gttctgattg ctaacttgcc agtgtttctc tttggggaat 1140
cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgaatttt ttttgtttcg 1200
ttgcataggg tttggtttgc ccttttcctt tatttcaata tatgccgtgc acttgtttgt 1260
cgggtcatct tttcatgttt tttttggctt ggttgtgatg atgtggtctg gttgggcggt 1320
cgttctagat cggagtagaa tactgtttca aactacctgg tggatttatt aaaggatctg 1380
tatgtatgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatatcgat 1440
ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttt 1500
ttcgcttggt tgtgatgatg tggtctggtc gggcggtcgt tctagatcgg agtagaatac 1560
tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtc atacatcttc 1620
atagttacga gtttaagatc gatggaaata tcgatctagg ataggtatac atgttgatgt 1680
gggttttact gatgcatata catggcatat gcagcatcta ttcatatgct ctaaccttga 1740
gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg 1800
gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt 1860
tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg atacttctgc 1920
aggtc 1925
<210> 123
<211> 850
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 123
gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca 60
tatttttttt tgtcacactt gtgtttgaag tgcagtttat ctatctctat acatatattt 120
aaacttcact atatgaataa tatagtctat agtattaaaa taatatcaat gttttagatg 180
attatataac tgaactgcta gacatggtct aaaggacaac cgagtatttt gacaacatga 240
ctctacagtt ttatcttttt agtgtgcatg tgttcttttt acttttgcaa atagcttcac 300
ctatataata cttcatccat tttattagta catccattta ctaaattttt agtacatcta 360
ttttattcta ttttagcctc taaattaaga aaacttaaac tctattttag ttttttattt 420
aataatttag atataaaata gaataaaata aagtgactaa aaaataacta aatacctttt 480
aagaaataaa aaaactaagg aaccattttt cttgttccga gtagataatg acagcctgtt 540
caacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc 600
aagcgaagca gacggcacgg catctctgta gctgcctctg gacccctctc gagagttccg 660
ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac 720
gtgagccggc acggcaggcg gcctcctctc acggcaccgg cagctacggg ggattccttt 780
cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagaccc cctccacacc 840
ctctttcccc 850
<210> 124
<211> 78
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 124
aacctcgtgt tcgttcggag cgcgcacaca cacaaccaga tctcccccaa atccacccgt 60
cggcacctcc gcttcaag 78
<210> 125
<211> 997
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 125
gtacgccgct catcctcctc ccccccctct ctctaccttc tctagatcgg cgtttcggtc 60
catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt 120
tagatccgtg ctgctagatt tcgtacacgg atgcgacctg tacatcagac atgttctgat 180
tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga 240
cgggatcgat ttcatgaatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc 300
tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgt tttttttggc 360
ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag aatactgttt 420
caaactacct ggtggattta ttaaaggatc tgtatgtatg tgccatacat cttcatagtt 480
acgagtttaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg 540
ttttactgat gcatatacag agatgctttt ttttcgcttg gttgtgatga tgtggtctgg 600
tcgggcggtc gttctagatc ggagtagaat actgtttcaa actacctggt ggatttatta 660
attttggatc tgtatgtgtg tcatacatct tcatagttac gagtttaaga tcgatggaaa 720
tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata tacatggcat 780
atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat 840
gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt 900
ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg 960
atgctcaccc tgttgtttgg tgatacttct gcaggtc 997
<210> 126
<211> 1925
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 126
gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca 60
tatttttttt tgtcacactt gtgtttgaag tgcagtttat ctatctctat acatatattt 120
aaacttcact atatgaataa tatagtctat agtattaaaa taatatcaat gttttagatg 180
attatataac tgaactgcta gacatggtct aaaggacaac cgagtatttt gacaacatga 240
ctctacagtt ttatcttttt agtgtgcatg tgttcttttt acttttgcaa atagcttcac 300
ctatataata cttcatccat tttattagta catccattta ctaaattttt agtacatcta 360
ttttattcta ttttagcctc taaattaaga aaacttaaac tctattttag ttttttattt 420
aataatttag atataaaata gaataaaata aagtgactaa aaaataacta aatacctttt 480
aagaaataaa aaaactaagg aaccattttt cttgttccga gtagataatg acagcctgtt 540
caacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc 600
aagcgaagca gacggcacgg catctctgta gctgcctctg gacccctctc gagagttccg 660
ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac 720
gtgagccggc acggcaggcg gcctcctctc acggcaccgg cagctacggg ggattccttt 780
cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagaccc cctccacacc 840
ctctttcccc aacctcgtgt tcgttcggag cgcgcacaca cacaaccaga tctcccccaa 900
atccacccgt cggcacctcc gcttcaaggt acgccgctca tcctcctccc ccccctctct 960
ctaccttctc tagatcggcg tttcggtcca tggttagggc ccggtagttc tacttctgtt 1020
catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagatttc gtacacggat 1080
gcgacctgta catcagacat gttctgattg ctaacttgcc agtgtttctc tttggggaat 1140
cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgaatttt ttttgtttcg 1200
ttgcataggg tttggtttgc ccttttcctt tatttcaata tatgccgtgc acttgtttgt 1260
cgggtcatct tttcatgttt tttttggctt ggttgtgatg atgtggtctg gttgggcggt 1320
cgttctagat cggagtagaa tactgtttca aactacctgg tggatttatt aaaggatctg 1380
tatgtatgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatatcgat 1440
ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttt 1500
ttcgcttggt tgtgatgatg tggtctggtc gggcggtcgt tctagatcgg agtagaatac 1560
tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtc atacatcttc 1620
atagttacga gtttaagatc gatggaaata tcgatctagg ataggtatac atgttgatgt 1680
gggttttact gatgcatata catggcatat gcagcatcta ttcatatgct ctaaccttga 1740
gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg 1800
gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt 1860
tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg atacttctgc 1920
agggt 1925
<210> 127
<211> 997
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 127
gtacgccgct catcctcctc ccccccctct ctctaccttc tctagatcgg cgtttcggtc 60
catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt 120
tagatccgtg ctgctagatt tcgtacacgg atgcgacctg tacatcagac atgttctgat 180
tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga 240
cgggatcgat ttcatgaatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc 300
tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgt tttttttggc 360
ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag aatactgttt 420
caaactacct ggtggattta ttaaaggatc tgtatgtatg tgccatacat cttcatagtt 480
acgagtttaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg 540
ttttactgat gcatatacag agatgctttt ttttcgcttg gttgtgatga tgtggtctgg 600
tcgggcggtc gttctagatc ggagtagaat actgtttcaa actacctggt ggatttatta 660
attttggatc tgtatgtgtg tcatacatct tcatagttac gagtttaaga tcgatggaaa 720
tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata tacatggcat 780
atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat 840
gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt 900
ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg 960
atgctcaccc tgttgtttgg tgatacttct gcagggt 997
<210> 128
<211> 1974
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 128
gtcgtgcccc tctctagaga taaagagcat tgcatgtcta agttataaaa aattaccaca 60
tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa 120
ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc 180
atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc 240
tacagtttta tctttttagt gtgcatgtgt tctccttttt tttttgcaaa tagcttcacc 300
tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt 360
tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa 420
ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa 480
gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc 540
ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa 600
ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg 660
ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca 720
tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc 780
ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 840
ctcgcccgcc gtaataaata gacaccccct ccacaccttc tttccccaac ctcgtgttgt 900
tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 960
caaggtacgc cgctcatcct cccccccccc tctctacctt ctctagatcg gcgttccggt 1020
ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg 1080
ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga 1140
ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag 1200
acgggatcga tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc 1260
tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt 1320
cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagaa gaattctgtt 1380
tcaaactacc tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat 1440
agttacgaat tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg 1500
cgggttttac tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt 1560
ctggttgggc ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg 1620
gtgtatttat taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt 1680
aagatggatg gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg 1740
catatacatg atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta 1800
ttataataaa caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat 1860
atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg 1920
tactgtttct tttgtcgatg ctcaccctgt tgtttggtga tacttctgca ggtc 1974
<210> 129
<211> 887
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 129
gtcgtgcccc tctctagaga taaagagcat tgcatgtcta agttataaaa aattaccaca 60
tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa 120
ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc 180
atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc 240
tacagtttta tctttttagt gtgcatgtgt tctccttttt tttttgcaaa tagcttcacc 300
tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt 360
tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa 420
ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa 480
gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc 540
ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa 600
ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg 660
ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca 720
tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc 780
ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 840
ctcgcccgcc gtaataaata gacaccccct ccacaccttc tttcccc 887
<210> 130
<211> 77
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 130
aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc 60
ggcacctccg cttcaag 77
<210> 131
<211> 1010
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 131
gtacgccgct catcctcccc cccccctctc taccttctct agatcggcgt tccggtccat 60
ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag 120
atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc 180
taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg 240
gatcgatttc atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta 300
tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg 360
gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagaagaat tctgtttcaa 420
actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt 480
acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg 540
ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtctgg 600
ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt 660
atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga 720
tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata 780
tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat 840
aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc 900
agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact 960
gtttcttttg tcgatgctca ccctgttgtt tggtgatact tctgcaggtc 1010
<210> 132
<211> 1974
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 132
gtcgtgcccc tctctagaga taaagagcat tgcatgtcta agttataaaa aattaccaca 60
tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa 120
ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc 180
atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc 240
tacagtttta tctttttagt gtgcatgtgt tctccttttt tttttgcaaa tagcttcacc 300
tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt 360
tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa 420
ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa 480
gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc 540
ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa 600
ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg 660
ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca 720
tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc 780
ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 840
ctcgcccgcc gtaataaata gacaccccct ccacaccttc tttccccaac ctcgtgttgt 900
tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 960
caaggtacgc cgctcatcct cccccccccc tctctacctt ctctagatcg gcgttccggt 1020
ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg 1080
ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga 1140
ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag 1200
acgggatcga tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc 1260
tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt 1320
cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagaa gaattctgtt 1380
tcaaactacc tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat 1440
agttacgaat tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg 1500
cgggttttac tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt 1560
ctggttgggc ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg 1620
gtgtatttat taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt 1680
aagatggatg gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg 1740
catatacatg atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta 1800
ttataataaa caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat 1860
atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg 1920
tactgtttct tttgtcgatg ctcaccctgt tgtttggtga tacttctgca gggt 1974
<210> 133
<211> 1010
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 133
gtacgccgct catcctcccc cccccctctc taccttctct agatcggcgt tccggtccat 60
ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag 120
atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc 180
taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg 240
gatcgatttc atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta 300
tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg 360
gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagaagaat tctgtttcaa 420
actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt 480
acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg 540
ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtctgg 600
ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt 660
atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga 720
tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata 780
tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat 840
aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc 900
agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact 960
gtttcttttg tcgatgctca ccctgttgtt tggtgatact tctgcagggt 1010
<210> 134
<211> 2008
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 134
gtcgtgcccc tctctagaga taaagagcat tgcatgtcta aagtataaaa aattaccaca 60
tatttttttg tcacacttat ttgaagtgta gtttatctat ctctatacat atatttaaac 120
ttcactctac aaataatata gtctataata ctaaaataat attagtgttt tagaggatca 180
tataaataaa ctgctagaca tggtctaaag gataattgaa tattttgaca atctacagtt 240
ttatcttttt agtgtgcatg tgatctctct gttttttttg caaatagctt gacctatata 300
atacttcatc cattttatta gtacatccat ttaggattta gggttgatgg tttctataga 360
ctaattttta gtacatccat tttattcttt ttagtctcta aattttttaa aactaaaact 420
ctattttagt tttttattta ataatttaga tataaaatga aataaaataa attgactaca 480
aataaaacaa atacccttta agaaataaaa aaactaagca aacatttttc ttgtttcgag 540
tagataatga caggctgttc aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc 600
agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtag ctgcctctgg 660
acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt 720
gcgtggcgga gcggcagacg tgaggcggca cggcaggcgg cctcttcctc ctctcacggc 780
accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc 840
gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttcg ttcggagcgc 900
acacacacgc aaccagatct cccccaaatc cagccgtcgg cacctccgct tcaaggtacg 960
ccgctcatcc tccccccccc cctctctcta ccttctctag atcggcgatc cggtccatgg 1020
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gagcaaacat gttcatgttc 1080
atgtttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag gatactgttt 1140
caagctacct ggtggattta ttaattttgt atctgtatgt gtgtgccata catcttcata 1200
gttacgagtt taagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc 1260
gggttttact gatgcatata cagagatgct ttttttctcg cttggttgtg atgatatggt 1320
ctggttgggc ggtcgttcta gatcggagta gaatactgtt tcaaactacc tggtggattt 1380
attaaaggat aaagggtcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgg 1440
atttattaaa ggatctgtat gtatgtgcct acatcttcat agttacgagt ttaagatgat 1500
ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat 1560
acagagatgc tttttttcgc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag 1620
atcggagtag aatactgttt caaactacct ggtggattta ttaattttgt atctttatgt 1680
gtgtgccata catcttcata gttacgagtt taagatgatg gatggaaata ttgatctagg 1740
ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcggcat 1800
ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa 1860
ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt 1920
agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcc gatgctcacc 1980
ctgttgttgg gtgatacttc tgcaggtc 2008
<210> 135
<211> 877
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 135
gtcgtgcccc tctctagaga taaagagcat tgcatgtcta aagtataaaa aattaccaca 60
tatttttttg tcacacttat ttgaagtgta gtttatctat ctctatacat atatttaaac 120
ttcactctac aaataatata gtctataata ctaaaataat attagtgttt tagaggatca 180
tataaataaa ctgctagaca tggtctaaag gataattgaa tattttgaca atctacagtt 240
ttatcttttt agtgtgcatg tgatctctct gttttttttg caaatagctt gacctatata 300
atacttcatc cattttatta gtacatccat ttaggattta gggttgatgg tttctataga 360
ctaattttta gtacatccat tttattcttt ttagtctcta aattttttaa aactaaaact 420
ctattttagt tttttattta ataatttaga tataaaatga aataaaataa attgactaca 480
aataaaacaa atacccttta agaaataaaa aaactaagca aacatttttc ttgtttcgag 540
tagataatga caggctgttc aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc 600
agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtag ctgcctctgg 660
acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt 720
gcgtggcgga gcggcagacg tgaggcggca cggcaggcgg cctcttcctc ctctcacggc 780
accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc 840
gtaataaata gacaccccct ccacaccctc tttcccc 877
<210> 136
<211> 78
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 136
aacctcgtgt tcgttcggag cgcacacaca cgcaaccaga tctcccccaa atccagccgt 60
cggcacctcc gcttcaag 78
<210> 137
<211> 1053
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 137
gtacgccgct catcctcccc ccccccctct ctctaccttc tctagatcgg cgatccggtc 60
catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagagca aacatgttca 120
tgttcatgtt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtaggatac 180
tgtttcaagc tacctggtgg atttattaat tttgtatctg tatgtgtgtg ccatacatct 240
tcatagttac gagtttaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt 300
gatgcgggtt ttactgatgc atatacagag atgctttttt tctcgcttgg ttgtgatgat 360
atggtctggt tgggcggtcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg 420
gatttattaa aggataaagg gtcgttctag atcggagtag aatactgttt caaactacct 480
ggtggattta ttaaaggatc tgtatgtatg tgcctacatc ttcatagtta cgagtttaag 540
atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg 600
catatacaga gatgcttttt ttcgcttggt tgtgatgatg tggtctggtt gggcggtcgt 660
tctagatcgg agtagaatac tgtttcaaac tacctggtgg atttattaat tttgtatctt 720
tatgtgtgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatattgat 780
ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc 840
ggcatctatt catatgctct aaccttgagt acctatctat tataataaac aagtatgttt 900
tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat 960
tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtccgatgc 1020
tcaccctgtt gttgggtgat acttctgcag gtc 1053
<210> 138
<211> 2008
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 138
gtcgtgcccc tctctagaga taaagagcat tgcatgtcta aagtataaaa aattaccaca 60
tatttttttg tcacacttat ttgaagtgta gtttatctat ctctatacat atatttaaac 120
ttcactctac aaataatata gtctataata ctaaaataat attagtgttt tagaggatca 180
tataaataaa ctgctagaca tggtctaaag gataattgaa tattttgaca atctacagtt 240
ttatcttttt agtgtgcatg tgatctctct gttttttttg caaatagctt gacctatata 300
atacttcatc cattttatta gtacatccat ttaggattta gggttgatgg tttctataga 360
ctaattttta gtacatccat tttattcttt ttagtctcta aattttttaa aactaaaact 420
ctattttagt tttttattta ataatttaga tataaaatga aataaaataa attgactaca 480
aataaaacaa atacccttta agaaataaaa aaactaagca aacatttttc ttgtttcgag 540
tagataatga caggctgttc aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc 600
agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtag ctgcctctgg 660
acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt 720
gcgtggcgga gcggcagacg tgaggcggca cggcaggcgg cctcttcctc ctctcacggc 780
accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc 840
gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttcg ttcggagcgc 900
acacacacgc aaccagatct cccccaaatc cagccgtcgg cacctccgct tcaaggtacg 960
ccgctcatcc tccccccccc cctctctcta ccttctctag atcggcgatc cggtccatgg 1020
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gagcaaacat gttcatgttc 1080
atgtttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag gatactgttt 1140
caagctacct ggtggattta ttaattttgt atctgtatgt gtgtgccata catcttcata 1200
gttacgagtt taagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc 1260
gggttttact gatgcatata cagagatgct ttttttctcg cttggttgtg atgatatggt 1320
ctggttgggc ggtcgttcta gatcggagta gaatactgtt tcaaactacc tggtggattt 1380
attaaaggat aaagggtcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgg 1440
atttattaaa ggatctgtat gtatgtgcct acatcttcat agttacgagt ttaagatgat 1500
ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat 1560
acagagatgc tttttttcgc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag 1620
atcggagtag aatactgttt caaactacct ggtggattta ttaattttgt atctttatgt 1680
gtgtgccata catcttcata gttacgagtt taagatgatg gatggaaata ttgatctagg 1740
ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcggcat 1800
ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa 1860
ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt 1920
agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcc gatgctcacc 1980
ctgttgttgg gtgatacttc tgcagggt 2008
<210> 139
<211> 1053
<212> DNA
<213> Zea mays subsp. Mexicana
<400> 139
gtacgccgct catcctcccc ccccccctct ctctaccttc tctagatcgg cgatccggtc 60
catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagagca aacatgttca 120
tgttcatgtt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtaggatac 180
tgtttcaagc tacctggtgg atttattaat tttgtatctg tatgtgtgtg ccatacatct 240
tcatagttac gagtttaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt 300
gatgcgggtt ttactgatgc atatacagag atgctttttt tctcgcttgg ttgtgatgat 360
atggtctggt tgggcggtcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg 420
gatttattaa aggataaagg gtcgttctag atcggagtag aatactgttt caaactacct 480
ggtggattta ttaaaggatc tgtatgtatg tgcctacatc ttcatagtta cgagtttaag 540
atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg 600
catatacaga gatgcttttt ttcgcttggt tgtgatgatg tggtctggtt gggcggtcgt 660
tctagatcgg agtagaatac tgtttcaaac tacctggtgg atttattaat tttgtatctt 720
tatgtgtgtg ccatacatct tcatagttac gagtttaaga tgatggatgg aaatattgat 780
ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc 840
ggcatctatt catatgctct aaccttgagt acctatctat tataataaac aagtatgttt 900
tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat 960
tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtccgatgc 1020
tcaccctgtt gttgggtgat acttctgcag ggt 1053
<210> 140
<211> 1635
<212> DNA
<213> Sorghum bicolor
<400> 140
ccaagtccaa atgtcaattc ccttgaagat gatctatttt tatcttttgc attttgttat 60
ggaagtttgc aaatagcaac aaatgctaag tcaatttgcc aaagtctttg gagatgctct 120
tagtctataa ttgaacaata tttgtaaaat acaaaaaaaa atagtactat ttttatttta 180
aaaaattttt ggaagtaaac aaggccgagg atggggaaac ggaagtccaa cacgtcgttt 240
tctaagttgg gctcaaaagc ccatcacgga actgacctgc tatgggtcgg aggagagcgc 300
gtccagatgg ttccagaggc tggtggtggt gggccaaacg cggaactccg ccaccgccac 360
ggcctcgtgc gcaagcgcag cgcgttgccg tgagccgtga cgtaaccctc cgttgcccac 420
gataaaagct ccacccccga ccccggcccc ccgatttccc ctacggacca gtctcccccc 480
gatcgcaatc gcgaattcgt cgcaccatcg gcacgcagac gaacgaagca aggctctccc 540
catcggctcg tcaaggtatg cgttccctag atttgttccc ttcctctctc ggtttgtcta 600
tatatatgca tgtatggtcg attcccgatc tcgtcgattc tcggtttcgc cttccgtacg 660
aagattcgtt tagattgttc atatgttctg ttgtgttacc agattgatcg gatcaacttg 720
atccagttat cttcgctcct ccgattagat ccgtttctat ttcagtatat atatactagt 780
atagtatcta gggttcacac tgttgaccga ctggttactt ggaattgatc cgtgctgagt 840
tcagttgttg ccgtccataa aggcccgtgc tattgtctgt tctgaaacga aatcctgtag 900
atttcttagg gttagtgttc aattcatcaa aaggttgatt agtgaattat caaatttgag 960
agggttaaat cattctcatc atgttgtctc gaatgtaatc ccaaagatat tatagactgt 1020
gtttcgattt gatggattga tttgtgtatc atctaaatca acaaggctaa gtcatcagtt 1080
catagaatca tgtttaggtt tccgttcaat agactagttt tatcaatata taaaattata 1140
agaagggtag ggtaaatcac gttgcctcaa atgccatcct gtatggtttg gtttcaattc 1200
aattagtttg gttgattagg gtatgctctg gattaagatg gttaaatctt ccctagcatc 1260
ttccctgcct atccttactt gatccgtttc ggatatgttg gaagtacagc gagcttattt 1320
catgttgata gtgacccctt tcagattata ctattgaata ttgtatgttt gccacttctg 1380
tatgttgaat tatcctgcta aattagcaat ggaattagca tattggcaat tggtatgcat 1440
ggacctaatc aggacggatg tggttatgtt agtttcaatt cattgtcaat tcattgttca 1500
cctgcgttag atatatatga tgatttttac gtgtagttca tagttcttga gttttggatc 1560
tttcttatct gatatatgct ttcctgtgcc tgtgctttat tgtgtcttac catgcgattt 1620
ttgtctatgc aggtc 1635
<210> 141
<211> 401
<212> DNA
<213> Sorghum bicolor
<400> 141
ccaagtccaa atgtcaattc ccttgaagat gatctatttt tatcttttgc attttgttat 60
ggaagtttgc aaatagcaac aaatgctaag tcaatttgcc aaagtctttg gagatgctct 120
tagtctataa ttgaacaata tttgtaaaat acaaaaaaaa atagtactat ttttatttta 180
aaaaattttt ggaagtaaac aaggccgagg atggggaaac ggaagtccaa cacgtcgttt 240
tctaagttgg gctcaaaagc ccatcacgga actgacctgc tatgggtcgg aggagagcgc 300
gtccagatgg ttccagaggc tggtggtggt gggccaaacg cggaactccg ccaccgccac 360
ggcctcgtgc gcaagcgcag cgcgttgccg tgagccgtga c 401
<210> 142
<211> 154
<212> DNA
<213> Sorghum bicolor
<400> 142
gtaaccctcc gttgcccacg ataaaagctc cacccccgac cccggccccc cgatttcccc 60
tacggaccag tctccccccg atcgcaatcg cgaattcgtc gcaccatcgg cacgcagacg 120
aacgaagcaa ggctctcccc atcggctcgt caag 154
<210> 143
<211> 1080
<212> DNA
<213> Sorghum bicolor
<400> 143
gtatgcgttc cctagatttg ttcccttcct ctctcggttt gtctatatat atgcatgtat 60
ggtcgattcc cgatctcgtc gattctcggt ttcgccttcc gtacgaagat tcgtttagat 120
tgttcatatg ttctgttgtg ttaccagatt gatcggatca acttgatcca gttatcttcg 180
ctcctccgat tagatccgtt tctatttcag tatatatata ctagtatagt atctagggtt 240
cacactgttg accgactggt tacttggaat tgatccgtgc tgagttcagt tgttgccgtc 300
cataaaggcc cgtgctattg tctgttctga aacgaaatcc tgtagatttc ttagggttag 360
tgttcaattc atcaaaaggt tgattagtga attatcaaat ttgagagggt taaatcattc 420
tcatcatgtt gtctcgaatg taatcccaaa gatattatag actgtgtttc gatttgatgg 480
attgatttgt gtatcatcta aatcaacaag gctaagtcat cagttcatag aatcatgttt 540
aggtttccgt tcaatagact agttttatca atatataaaa ttataagaag ggtagggtaa 600
atcacgttgc ctcaaatgcc atcctgtatg gtttggtttc aattcaatta gtttggttga 660
ttagggtatg ctctggatta agatggttaa atcttcccta gcatcttccc tgcctatcct 720
tacttgatcc gtttcggata tgttggaagt acagcgagct tatttcatgt tgatagtgac 780
ccctttcaga ttatactatt gaatattgta tgtttgccac ttctgtatgt tgaattatcc 840
tgctaaatta gcaatggaat tagcatattg gcaattggta tgcatggacc taatcaggac 900
ggatgtggtt atgttagttt caattcattg tcaattcatt gttcacctgc gttagatata 960
tatgatgatt tttacgtgta gttcatagtt cttgagtttt ggatctttct tatctgatat 1020
atgctttcct gtgcctgtgc tttattgtgt cttaccatgc gatttttgtc tatgcaggtc 1080
<210> 144
<211> 2067
<212> DNA
<213> Sorghum bicolor
<400> 144
cattaaaagt cattatgtgc atgcgtcgta actaacatgg atatgttgct gcactatctc 60
ctcgcactag ctgcgcatga taaagccaca agccaaaatt aattattatg ggtgagaata 120
aatacgtacc agcaccggcc atagaaaaag tacattatta aaggtctaat ttggaaacag 180
tctgaaaacg acgtgcgctg cagaggtaaa tgtaattttc ggcactaaaa ccattatcaa 240
ctaattcatt caataacagt tatttagaaa atgtatagct cgctctaaaa aaacagttta 300
gaaaaacagt caaaataatt cgaccaacaa acagttaata aggttcatta aatatataat 360
gcacggtgct atttgatctt ttaaaggaaa aagaggaata gtcgtgggcg ccaggcggga 420
attggggcgc gggagtctgc cggacgacgc gttccgtccg aacggccgga cccgacgagg 480
cccccccgcc gccccacgtc gcagaaccgt ccgtgggtgg taatctggcc gggtacacca 540
gccgtcccct tgggcggcct cacagcactg ggctcacacg tgagttttgt tctgggcttc 600
ggatcgcacc atatgggcct cggcatcaga aagacggggc ccgtctggga tagaagagac 660
aggaacctcc tcgtggattc cagaagccag ccacgagcga ccaccgacgc ggaggatact 720
cgtcgtccaa gtccaacacg gcgggcgggc gggcggacgc gtgggctggg ctaactgcct 780
aaccttaacc tccaaggcac gccaaggccc gcttctccca cccgacataa atatcccccc 840
atccaggcaa ggcgcagagc ctcagaccag attccgatca atcacccata agctcccccc 900
aaatctgttc ctcgtctccc gtctcgcggt ttcctacttc cctcggacgc ctccggcaag 960
tcgctcgacc gcgcgattcc gcccgctcaa ggtatcaact cggttcacca ctccaatcta 1020
cgtctgattt agatgttact tccatctatg tctaatttag atgttactcc gatgcgattg 1080
gattatgttt atgcggtttg cactgctctg gaaactggaa tctagggttt cgagtgattt 1140
gatcgatcgc gatctgtgat ttcgttgcgc cttgtgtatg cttggagtga tctaggcttg 1200
tatatgcggc atcgcgatct gacgcggttg ctttgtagag gctgggggtc taggctgtga 1260
ttttagaatc aaataaagct gttccttacc gtagatgttt cctacatgtt ctgtccagta 1320
ctccagtgct atattcacat tgtttgaggc ttgagttttg tcgatcagtg gtcatgagaa 1380
aaatatatct catgatttta gaggcaccta ttgggaaagg tagatggttc cgttttacat 1440
gttttataga ccttgtggca tggctccttt gttctatggg tgctttattt tcctgaataa 1500
cagtaatgcg agactggtct atgggtgctt tgaccagtaa tgcgagacta gttatttgat 1560
catggtgcag ttcctagtga ttacgaacaa caatttggta gctcagttca ttcagcattg 1620
gtttctacga tccttatcat tttacttctg aatgaattta tttatttaag atattacagt 1680
gcaataaact gctgtataat atcagtaaca aactgctatt actagtaaat gcctagattc 1740
ataataattc attattctac ttgaaaatga tcttaggcct ttttatgcgg tcctacgcat 1800
ccttccacag gacttgctgt ttgtttgttt tttgtaatcc ctcgctggga cgcagaatgg 1860
ttcatctgtg ctaataattt ttttgcatat ataagtttat agttctcatt attcatgtgg 1920
ctatggtagc ctgtaaaatc tattgtaata acatattagt cagccataca tctgttccaa 1980
cttgctcaat tgcaaatcat atctccactt aaagcacatg tttgcaagct ttctgacaag 2040
tttctttgtg tttgattgaa acaggtg 2067
<210> 145
<211> 855
<212> DNA
<213> Sorghum bicolor
<400> 145
cattaaaagt cattatgtgc atgcgtcgta actaacatgg atatgttgct gcactatctc 60
ctcgcactag ctgcgcatga taaagccaca agccaaaatt aattattatg ggtgagaata 120
aatacgtacc agcaccggcc atagaaaaag tacattatta aaggtctaat ttggaaacag 180
tctgaaaacg acgtgcgctg cagaggtaaa tgtaattttc ggcactaaaa ccattatcaa 240
ctaattcatt caataacagt tatttagaaa atgtatagct cgctctaaaa aaacagttta 300
gaaaaacagt caaaataatt cgaccaacaa acagttaata aggttcatta aatatataat 360
gcacggtgct atttgatctt ttaaaggaaa aagaggaata gtcgtgggcg ccaggcggga 420
attggggcgc gggagtctgc cggacgacgc gttccgtccg aacggccgga cccgacgagg 480
cccccccgcc gccccacgtc gcagaaccgt ccgtgggtgg taatctggcc gggtacacca 540
gccgtcccct tgggcggcct cacagcactg ggctcacacg tgagttttgt tctgggcttc 600
ggatcgcacc atatgggcct cggcatcaga aagacggggc ccgtctggga tagaagagac 660
aggaacctcc tcgtggattc cagaagccag ccacgagcga ccaccgacgc ggaggatact 720
cgtcgtccaa gtccaacacg gcgggcgggc gggcggacgc gtgggctggg ctaactgcct 780
aaccttaacc tccaaggcac gccaaggccc gcttctccca cccgacataa atatcccccc 840
atccaggcaa ggcgc 855
<210> 146
<211> 136
<212> DNA
<213> Sorghum bicolor
<400> 146
agagcctcag accagattcc gatcaatcac ccataagctc cccccaaatc tgttcctcgt 60
ctcccgtctc gcggtttcct acttccctcg gacgcctccg gcaagtcgct cgaccgcgcg 120
attccgcccg ctcaag 136
<210> 147
<211> 1076
<212> DNA
<213> Sorghum bicolor
<400> 147
gtatcaactc ggttcaccac tccaatctac gtctgattta gatgttactt ccatctatgt 60
ctaatttaga tgttactccg atgcgattgg attatgttta tgcggtttgc actgctctgg 120
aaactggaat ctagggtttc gagtgatttg atcgatcgcg atctgtgatt tcgttgcgcc 180
ttgtgtatgc ttggagtgat ctaggcttgt atatgcggca tcgcgatctg acgcggttgc 240
tttgtagagg ctgggggtct aggctgtgat tttagaatca aataaagctg ttccttaccg 300
tagatgtttc ctacatgttc tgtccagtac tccagtgcta tattcacatt gtttgaggct 360
tgagttttgt cgatcagtgg tcatgagaaa aatatatctc atgattttag aggcacctat 420
tgggaaaggt agatggttcc gttttacatg ttttatagac cttgtggcat ggctcctttg 480
ttctatgggt gctttatttt cctgaataac agtaatgcga gactggtcta tgggtgcttt 540
gaccagtaat gcgagactag ttatttgatc atggtgcagt tcctagtgat tacgaacaac 600
aatttggtag ctcagttcat tcagcattgg tttctacgat ccttatcatt ttacttctga 660
atgaatttat ttatttaaga tattacagtg caataaactg ctgtataata tcagtaacaa 720
actgctatta ctagtaaatg cctagattca taataattca ttattctact tgaaaatgat 780
cttaggcctt tttatgcggt cctacgcatc cttccacagg acttgctgtt tgtttgtttt 840
ttgtaatccc tcgctgggac gcagaatggt tcatctgtgc taataatttt tttgcatata 900
taagtttata gttctcatta ttcatgtggc tatggtagcc tgtaaaatct attgtaataa 960
catattagtc agccatacat ctgttccaac ttgctcaatt gcaaatcata tctccactta 1020
aagcacatgt ttgcaagctt tctgacaagt ttctttgtgt ttgattgaaa caggtg 1076
<210> 148
<211> 2067
<212> DNA
<213> Sorghum bicolor
<400> 148
cattaaaagt cattatgtgc atgcgtcgta actaacatgg atatgttgct gcactatctc 60
ctcgcactag ctgcgcatga taaagccaca agccaaaatt aattattatg ggtgagaata 120
aatacgtacc agcaccggcc atagaaaaag tacattatta aaggtctaat ttggaaacag 180
tctgaaaacg acgtgcgctg cagaggtaaa tgtaattttc ggcactaaaa ccattatcaa 240
ctaattcatt caataacagt tatttagaaa atgtatagct cgctctaaaa aaacagttta 300
gaaaaacagt caaaataatt cgaccaacaa acagttaata aggttcatta aatatataat 360
gcacggtgct atttgatctt ttaaaggaaa aagaggaata gtcgtgggcg ccaggcggga 420
attggggcgc gggagtctgc cggacgacgc gttccgtccg aacggccgga cccgacgagg 480
cccccccgcc gccccacgtc gcagaaccgt ccgtgggtgg taatctggcc gggtacacca 540
gccgtcccct tgggcggcct cacagcactg ggctcacacg tgagttttgt tctgggcttc 600
ggatcgcacc atatgggcct cggcatcaga aagacggggc ccgtctggga tagaagagac 660
aggaacctcc tcgtggattc cagaagccag ccacgagcga ccaccgacgc ggaggatact 720
cgtcgtccaa gtccaacacg gcgggcgggc gggcggacgc gtgggctggg ctaactgcct 780
aaccttaacc tccaaggcac gccaaggccc gcttctccca cccgacataa atatcccccc 840
atccaggcaa ggcgcagagc ctcagaccag attccgatca atcacccata agctcccccc 900
aaatctgttc ctcgtctccc gtctcgcggt ttcctacttc cctcggacgc ctccggcaag 960
tcgctcgacc gcgcgattcc gcccgctcaa ggtatcaact cggttcacca ctccaatcta 1020
cgtctgattt agatgttact tccatctatg tctaatttag atgttactcc gatgcgattg 1080
gattatgttt atgcggtttg cactgctctg gaaactggaa tctagggttt cgagtgattt 1140
gatcgatcgc gatctgtgat ttcgttgcgc cttgtgtatg cttggagtga tctaggcttg 1200
tatatgcggc atcgcgatct gacgcggttg ctttgtagag gctgggggtc taggctgtga 1260
ttttagaatc aaataaagct gttccttacc gtagatgttt cctacatgtt ctgtccagta 1320
ctccagtgct atattcacat tgtttgaggc ttgagttttg tcgatcagtg gtcatgagaa 1380
aaatatatct catgatttta gaggcaccta ttgggaaagg tagatggttc cgttttacat 1440
gttttataga ccttgtggca tggctccttt gttctatggg tgctttattt tcctgaataa 1500
cagtaatgcg agactggtct atgggtgctt tgaccagtaa tgcgagacta gttatttgat 1560
catggtgcag ttcctagtga ttacgaacaa caatttggta gctcagttca ttcagcattg 1620
gtttctacga tccttatcat tttacttctg aatgaattta tttatttaag atattacagt 1680
gcaataaact gctgtataat atcagtaaca aactgctatt actagtaaat gcctagattc 1740
ataataattc attattctac ttgaaaatga tcttaggcct ttttatgcgg tcctacgcat 1800
ccttccacag gacttgctgt ttgtttgttt tttgtaatcc ctcgctggga cgcagaatgg 1860
ttcatctgtg ctaataattt ttttgcatat ataagtttat agttctcatt attcatgtgg 1920
ctatggtagc ctgtaaaatc tattgtaata acatattagt cagccataca tctgttccaa 1980
cttgctcaat tgcaaatcat atctccactt aaagcacatg tttgcaagct ttctgacaag 2040
tttctttgtg tttgattgaa acagggt 2067
<210> 149
<211> 1076
<212> DNA
<213> Sorghum bicolor
<400> 149
gtatcaactc ggttcaccac tccaatctac gtctgattta gatgttactt ccatctatgt 60
ctaatttaga tgttactccg atgcgattgg attatgttta tgcggtttgc actgctctgg 120
aaactggaat ctagggtttc gagtgatttg atcgatcgcg atctgtgatt tcgttgcgcc 180
ttgtgtatgc ttggagtgat ctaggcttgt atatgcggca tcgcgatctg acgcggttgc 240
tttgtagagg ctgggggtct aggctgtgat tttagaatca aataaagctg ttccttaccg 300
tagatgtttc ctacatgttc tgtccagtac tccagtgcta tattcacatt gtttgaggct 360
tgagttttgt cgatcagtgg tcatgagaaa aatatatctc atgattttag aggcacctat 420
tgggaaaggt agatggttcc gttttacatg ttttatagac cttgtggcat ggctcctttg 480
ttctatgggt gctttatttt cctgaataac agtaatgcga gactggtcta tgggtgcttt 540
gaccagtaat gcgagactag ttatttgatc atggtgcagt tcctagtgat tacgaacaac 600
aatttggtag ctcagttcat tcagcattgg tttctacgat ccttatcatt ttacttctga 660
atgaatttat ttatttaaga tattacagtg caataaactg ctgtataata tcagtaacaa 720
actgctatta ctagtaaatg cctagattca taataattca ttattctact tgaaaatgat 780
cttaggcctt tttatgcggt cctacgcatc cttccacagg acttgctgtt tgtttgtttt 840
ttgtaatccc tcgctgggac gcagaatggt tcatctgtgc taataatttt tttgcatata 900
taagtttata gttctcatta ttcatgtggc tatggtagcc tgtaaaatct attgtaataa 960
catattagtc agccatacat ctgttccaac ttgctcaatt gcaaatcata tctccactta 1020
aagcacatgt ttgcaagctt tctgacaagt ttctttgtgt ttgattgaaa cagggt 1076
<210> 150
<211> 2003
<212> DNA
<213> Sorghum bicolor
<400> 150
agaagtaaaa aaaaagttcg tttcagaatc ataaaggtaa gttaaaaaaa gaccatacaa 60
aaaagaggta tttaatgata aactataatc cagaatttgt taggatagta tataagaata 120
agaccttgtt tagtttcaaa aaaatttgca aaattttcca gattcctcgt cacatcaaat 180
ctttagaggt atgcatggag tattaaatat agacaagacc taaataagaa aacatgaaat 240
gttcacgaaa aaaatcaagc caatgcatga tcgaagcaaa cggtatagta acggtgttaa 300
cctgatccat tgatctttgt aatctttaac ggccacctac cgcgggcagc aaacggcgtc 360
cccctcctcg atatctccgc ggcggcctct ggctttttcc gcggaattgc gcggtgggga 420
cggattccac gagaccgcaa cgcaaccgcc tctcgccgct gggccccaca ccgctcggtg 480
ccgtagcccg tagcctcacg ggattctttc tccctcctcc cccgtgtata aattggcttc 540
atcccctccc tgcctcatcc atccaaatcc cactccccaa tcccatcccg tcggagaaat 600
tcatcgaagc gaagcgaagc gaatcctccc gatcctctca aggtacgcga gttttcgaat 660
cccctccaga cccctcgtat gctttccctg ttcgttttcg tcgtagcgtt tgattaggta 720
tgctttccct gttcgtgttc gtcgtagggt tcgattaggt cgtgtgaggc catggcctgc 780
tgtgataaat ttatttgttg ttatatcgga tctgtagtcg atttgggggt cgtggtgtag 840
atccgcgggc tgtgatgaag ttatttggtg tgattgtgct cgcgtgattc tgcgcgttga 900
gctcgagtag atctgatggt tggacgaccg attggttcgt tggctggctg cgctaaggtt 960
gggctgggct catgttgcgt tcgctgttgc gcgtgattcc gcggatggac ttgcgcttga 1020
ttgccgccag atcacgttac gattatgtga tttcgtttgg aactttttag atttgtagct 1080
tctgcttatt atatgacaga tgcgcctact gctcatatgc ctgtggtaaa taatggatgg 1140
ctgtgggtca aactagttga ttgtcgagtc atgtatcata tacaggtgta tagacttgcg 1200
tctaattgtt tgcatgttgc agttatatga tttgttttag attgtttgtt ccactcatct 1260
aggctgtaaa agggacacta cttattagct tgttgtttaa tctttttatt agtagattat 1320
attggtaatg ttttactaat tattattatg ttatatgtga cttctgctca tgcctgatta 1380
taatcataga tcactgtagt tgattgttga atcatgtgtc aaatacccgt atacataaca 1440
ctacacattt gcttagttgt ttccttaact catgcaaatt gaacaccatg tatgatttgc 1500
atggtgctgt aatgttaaat actacagtcc tgttggtact tgtttagtaa gaatctgctt 1560
catacaacta tatgctatgc ctgatgataa tcatatatct ttgtgtaatt aataattagt 1620
tgactgttga ataatgtatc gagtacatac catggcacaa ttgcttagtc acttccttaa 1680
ccatgcatat tgaactgacc ccttcatgtt ctgctgaatt gttctattct gattagacca 1740
tacatcatgt attgcaatct ttatttgcaa ttgtaatgta atggttcggt tctcaaatgt 1800
taaatgctat agttgtgcta ctttctaatg ttaaatgcta tagctgtgct acttgtaaga 1860
tctgcttcat agtttagtta aattaggatg atgagctttg atgctgtaac tttgtttgat 1920
tatgttcata gttgatcagt ttttgttaga ctcacagtaa cttatggtct cactcttctt 1980
ctggtctttg atgtttgcag cgg 2003
<210> 151
<211> 565
<212> DNA
<213> Sorghum bicolor
<400> 151
agaagtaaaa aaaaagttcg tttcagaatc ataaaggtaa gttaaaaaaa gaccatacaa 60
aaaagaggta tttaatgata aactataatc cagaatttgt taggatagta tataagaata 120
agaccttgtt tagtttcaaa aaaatttgca aaattttcca gattcctcgt cacatcaaat 180
ctttagaggt atgcatggag tattaaatat agacaagacc taaataagaa aacatgaaat 240
gttcacgaaa aaaatcaagc caatgcatga tcgaagcaaa cggtatagta acggtgttaa 300
cctgatccat tgatctttgt aatctttaac ggccacctac cgcgggcagc aaacggcgtc 360
cccctcctcg atatctccgc ggcggcctct ggctttttcc gcggaattgc gcggtgggga 420
cggattccac gagaccgcaa cgcaaccgcc tctcgccgct gggccccaca ccgctcggtg 480
ccgtagcccg tagcctcacg ggattctttc tccctcctcc cccgtgtata aattggcttc 540
atcccctccc tgcctcatcc atcca 565
<210> 152
<211> 77
<212> DNA
<213> Sorghum bicolor
<400> 152
aatcccactc cccaatccca tcccgtcgga gaaattcatc gaagcgaagc gaagcgaatc 60
ctcccgatcc tctcaag 77
<210> 153
<211> 1361
<212> DNA
<213> Sorghum bicolor
<400> 153
gtacgcgagt tttcgaatcc cctccagacc cctcgtatgc tttccctgtt cgttttcgtc 60
gtagcgtttg attaggtatg ctttccctgt tcgtgttcgt cgtagggttc gattaggtcg 120
tgtgaggcca tggcctgctg tgataaattt atttgttgtt atatcggatc tgtagtcgat 180
ttgggggtcg tggtgtagat ccgcgggctg tgatgaagtt atttggtgtg attgtgctcg 240
cgtgattctg cgcgttgagc tcgagtagat ctgatggttg gacgaccgat tggttcgttg 300
gctggctgcg ctaaggttgg gctgggctca tgttgcgttc gctgttgcgc gtgattccgc 360
ggatggactt gcgcttgatt gccgccagat cacgttacga ttatgtgatt tcgtttggaa 420
ctttttagat ttgtagcttc tgcttattat atgacagatg cgcctactgc tcatatgcct 480
gtggtaaata atggatggct gtgggtcaaa ctagttgatt gtcgagtcat gtatcatata 540
caggtgtata gacttgcgtc taattgtttg catgttgcag ttatatgatt tgttttagat 600
tgtttgttcc actcatctag gctgtaaaag ggacactact tattagcttg ttgtttaatc 660
tttttattag tagattatat tggtaatgtt ttactaatta ttattatgtt atatgtgact 720
tctgctcatg cctgattata atcatagatc actgtagttg attgttgaat catgtgtcaa 780
atacccgtat acataacact acacatttgc ttagttgttt ccttaactca tgcaaattga 840
acaccatgta tgatttgcat ggtgctgtaa tgttaaatac tacagtcctg ttggtacttg 900
tttagtaaga atctgcttca tacaactata tgctatgcct gatgataatc atatatcttt 960
gtgtaattaa taattagttg actgttgaat aatgtatcga gtacatacca tggcacaatt 1020
gcttagtcac ttccttaacc atgcatattg aactgacccc ttcatgttct gctgaattgt 1080
tctattctga ttagaccata catcatgtat tgcaatcttt atttgcaatt gtaatgtaat 1140
ggttcggttc tcaaatgtta aatgctatag ttgtgctact ttctaatgtt aaatgctata 1200
gctgtgctac ttgtaagatc tgcttcatag tttagttaaa ttaggatgat gagctttgat 1260
gctgtaactt tgtttgatta tgttcatagt tgatcagttt ttgttagact cacagtaact 1320
tatggtctca ctcttcttct ggtctttgat gtttgcagcg g 1361
<210> 154
<211> 1812
<212> DNA
<213> Artificial sequence
<220>
<221> misc_feature
<222> (1)..(1812)
<223> Codon optimized coding sequence.
<400> 154
atggtccgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca 60
ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa 120
gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt 180
cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca 240
ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat 300
aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg 360
tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga actgaactgg 420
cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa gcagtcttac 480
ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta caccacgccg 540
aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg taaccacgcg 600
tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg tgatgcggat 660
caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt gaatccgcac 720
ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa aagccagaca 780
gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa gggcgaacag 840
ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga agatgcggac 900
ttgcgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt aatggactgg 960
attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat gctcgactgg 1020
gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt taacctctct 1080
ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga agaggcagtc 1140
aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc gcgtgacaaa 1200
aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg tccgcaaggt 1260
gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc gacgcgtccg 1320
atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag cgatctcttt 1380
gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga tttggaaacg 1440
gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca tcagccgatt 1500
atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta caccgacatg 1560
tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt tgatcgcgtc 1620
agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc gcaaggcata 1680
ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc gaagtcggcg 1740
gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc gcagcaggga 1800
ggcaaacaat ga 1812
<210> 155
<211> 2001
<212> DNA
<213> Artificial sequence
<220>
<221> misc_feature
<222> (1)..(2001)
<223> Chimeric coding sequence with processable intron.
<400> 155
atggtccgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg cctgtgggca 60
ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag cgcgttacaa 120
gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga tgcagatatt 180
cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa aggttgggca 240
ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt gtgggtcaat 300
aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga tgtcacgccg 360
tatgttattg ccgggaaaag tgtacgtaag tttctgcttc tacctttgat atatatataa 420
taattatcat taattagtag taatataata tttcaaatat ttttttcaaa ataaaagaat 480
gtagtatata gcaattgctt ttctgtagtt tataagtgtg tatattttaa tttataactt 540
ttctaatata tgaccaaaat ttgttgatgt gcaggtatca ccgtttgtgt gaacaacgaa 600
ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg caagaaaaag 660
cagtcttact tccatgattt ctttaactat gccggaatcc atcgcagcgt aatgctctac 720
accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc gcaagactgt 780
aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt 840
gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt gcaagtggtg 900
aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt cacagccaaa 960
agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag 1020
ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa 1080
gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga ccacgcatta 1140
atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc tgaagagatg 1200
ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt 1260
aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa 1320
gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga gctgatagcg 1380
cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc ggatacccgt 1440
ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa actcgacccg 1500
acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc 1560
gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat 1620
ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat 1680
cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac 1740
accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt 1800
gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt tgcgacctcg 1860
caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg 1920
aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg 1980
cagcagggag gcaaacaatg a 2001
<210> 156
<211> 1653
<212> DNA
<213> Artificial sequence
<220>
<221> misc_feature
<222> (1)..(1653)
<223> Codon optimized coding sequence.
<400> 156
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga 60
accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt 120
gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc 180
gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta 240
tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt 300
gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt 360
tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa 420
aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga 480
tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat 540
tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga 600
tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg 660
catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt 720
gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt 780
cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac 840
aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg 900
attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg 960
aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat 1020
gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc 1080
gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa 1140
acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt 1200
tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct 1260
ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct 1320
ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa 1380
caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt 1440
cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat 1500
tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac 1560
gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata 1620
aaggccaaga agggcggaaa gtccaaattg taa 1653
<210> 157
<211> 936
<212> DNA
<213> Artificial sequence
<220>
<221> misc_feature
<222> (1)..(936)
<223> Codon optimized coding sequence.
<400> 157
atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg 60
tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag 120
aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg 180
aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga 240
atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac 300
ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac 360
tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc 420
gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag 480
gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc 540
ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct 600
gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct 660
cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac 720
aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg 780
ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag 840
gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag 900
agcttcgtgg agcgcgtgct gaagaacgag cagtaa 936
<210> 158
<211> 253
<212> DNA
<213> Agrobacterium tumefaciens
<400> 158
gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg 60
atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc 120
atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac 180
gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct 240
atgttactag atc 253
<210> 159
<211> 210
<212> DNA
<213> Triticum aestivum
<400> 159
ctgcatgcgt ttggacgtat gctcattcag gttggagcca atttggttga tgtgtgtgcg 60
agttcttgcg agtctgatga gacatctctg tattgtgttt ctttccccag tgttttctgt 120
acttgtgtaa tcggctaatc gccaacagat tcggcgatga ataaatgaga aataaattgt 180
tctgattttg agtgcaaaaa aaaaggaatt 210
<210> 160
<211> 300
<212> DNA
<213> Oryza sativa
<400> 160
attaatcgat cctccgatcc cttaattacc ataccattac accatgcatc aatatccata 60
tatatataaa ccctttcgca cgtacttata ctatgttttg tcatacatat atatgtgtcg 120
aacgatcgat ctatcactga tatgatatga ttgatccatc agcctgatct ctgtatcttg 180
ttatttgtat accgtcaaat aaaagtttct tccacttgtg ttaataatta gctactctca 240
tctcatgaac cctatatata actagtttaa tttgctgtca attgaacatg atgatcgatg 300
<210> 161
<211> 1204
<212> DNA
<213> Artificial sequence
<220>
<221> misc_feature
<222> (1)..(1204)
<223> Chimeric transcriptional regulatory expression element group.
<400> 161
ggtccgattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc 60
ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc 120
catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa 180
gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca 240
aagcaagtgg attgatgtga tggtccgatt gagacttttc aacaaagggt aatatccgga 300
aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag 360
gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc 420
tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 480
gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 540
gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 600
catttggaga ggacacgctg acaagctgac tctagcagat cctctagaac catcttccac 660
acactcaagc cacactattg gagaacacac agggacaaca caccataaga tccaagggag 720
gcctccgccg ccgccggtaa ccaccccgcc cctctcctct ttctttctcc gttttttttt 780
ccgtctcggt ctcgatcttt ggccttggta gtttgggtgg gcgagaggcg gcttcgtgcg 840
cgcccagatc ggtgcgcggg aggggcggga tctcgcggct ggggctctcg ccggcgtgga 900
tccggcccgg atctcgcggg gaatggggct ctcggatgta gatctgcgat ccgccgttgt 960
tgggggagat gatggggggt ttaaaatttc cgccgtgcta aacaagatca ggaagagggg 1020
aaaagggcac tatggtttat atttttatat atttctgctg cttcgtcagg cttagatgtg 1080
ctagatcttt ctttcttctt tttgtgggta gaatttgaat ccctcagcat tgttcatcgg 1140
tagtttttct tttcatgatt tgtgacaaat gcagcctcgt gcggagcttt tttgtaggta 1200
gaag 1204
<210> 162
<211> 1396
<212> DNA
<213> Oryza sativa
<400> 162
tcgaggtcat tcatatgctt gagaagagag tcgggatagt ccaaaataaa acaaaggtaa 60
gattacctgg tcaaaagtga aaacatcagt taaaaggtgg tataaagtaa aatatcggta 120
ataaaaggtg gcccaaagtg aaatttactc ttttctacta ttataaaaat tgaggatgtt 180
tttgtcggta ctttgatacg tcatttttgt atgaattggt ttttaagttt attcgctttt 240
ggaaatgcat atctgtattt gagtcgggtt ttaagttcgt ttgcttttgt aaatacagag 300
ggatttgtat aagaaatatc tttagaaaaa cccatatgct aatttgacat aatttttgag 360
aaaaatatat attcaggcga attctcacaa tgaacaataa taagattaaa atagctttcc 420
cccgttgcag cgcatgggta ttttttctag taaaaataaa agataaactt agactcaaaa 480
catttacaaa aacaacccct aaagttccta aagcccaaag tgctatccac gatccatagc 540
aagcccagcc caacccaacc caacccagcc caccccagtc cagccaactg gacaatagtc 600
tccacacccc cccactatca ccgtgagttg tccgcacgca ccgcacgtct cgcagccaaa 660
aaaaaaaaga aagaaaaaaa agaaaaagaa aaaacagcag gtgggtccgg gtcgtggggg 720
ccggaaacgc gaggaggatc gcgagccagc gacgaggccg gccctccctc cgcttccaaa 780
gaaacgcccc ccatcgccac tatatacata cccccccctc tcctcccatc cccccaaccc 840
taccaccacc accaccacca cctccacctc ctcccccctc gctgccggac gacgagctcc 900
tcccccctcc ccctccgccg ccgccgcgcc ggtaaccacc ccgcccctct cctctttctt 960
tctccgtttt tttttccgtc tcggtctcga tctttggcct tggtagtttg ggtgggcgag 1020
aggcggcttc gtgccgccca gatcggtgcg cgggaggggc gggatctcgc ggctggctct 1080
cgcccccgtg gatccggccc ggatctcgcg gggaatgggg ctctcggatg tagatctgcg 1140
atccgccgtt gttggggccg atgatggggc ccttaaaatt tccgccgtgc taaacaagat 1200
caggaagagg ggaaaagggc actatggttt atatttttat atatttctgc tgcttcgtca 1260
ggcttagatg tgctagatct ttctttcttc tttttgtggg tagaatttaa tccctcagca 1320
ttgttcatcg gtagtttttc ttttcatgat tcgtgacaaa tgcagcctcg tgcggacgtt 1380
tttttgtagg tagaag 1396
<210> 163
<211> 1446
<212> DNA
<213> Artificial sequence
<220>
<221> misc_feature
<222> (1)..(1446)
<223> Chimeric transcriptional regulatory expression element group.
<400> 163
ggtccgattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc 60
ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc 120
catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa 180
gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca 240
aagcaagtgg attgatgtga tggtccgatt gagacttttc aacaaagggt aatatccgga 300
aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag 360
gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc 420
tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa 480
gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg 540
gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt 600
catttggaga ggacacgctg acaagctgac tctagcagat ctaccgtctt cggtacgcgc 660
tcactccgcc ctctgccttt gttactgcca cgtttctctg aatgctctct tgtgtggtga 720
ttgctgagag tggtttagct ggatctagaa ttacactctg aaatcgtgtt ctgcctgtgc 780
tgattacttg ccgtcctttg tagcagcaaa atatagggac atggtagtac gaaacgaaga 840
tagaacctac acagcaatac gagaaatgtg taatttggtg cttagcggta tttatttaag 900
cacatgttgg tgttataggg cacttggatt cagaagtttg ctgttaattt aggcacaggc 960
ttcatactac atgggtcaat agtataggga ttcatattat aggcgatact ataataattt 1020
gttcgtctgc agagcttatt atttgccaaa attagatatt cctattctgt ttttgtttgt 1080
gtgctgttaa attgttaacg cctgaaggaa taaatataaa tgacgaaatt ttgatgttta 1140
tctctgctcc tttattgtga ccataagtca agatcagatg cacttgtttt aaatattgtt 1200
gtctgaagaa ataagtactg acagtatttt gatgcattga tctgcttgtt tgttgtaaca 1260
aaatttaaaa ataaagagtt tcctttttgt tgctctcctt acctcctgat ggtatctagt 1320
atctaccaac tgacactata ttgcttctct ttacatacgt atcttgctcg atgccttctc 1380
cctagtgttg accagtgtta ctcacatagt ctttgctcat ttcattgtaa tgcagatacc 1440
aagcgg 1446
<210> 164
<211> 675
<212> DNA
<213> Artificial sequence
<220>
<221> misc_feature
<222> (1)..(675)
<223> Chimeric transcriptional regulatory expression element group.
<400> 164
ggtccgatgt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg 60
cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg 120
ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa 180
agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc 240
aaagcaagtg gattgatgtg atggtccgat gtgagacttt tcaacaaagg gtaatatccg 300
gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa 360
aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg 420
cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag 480
aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa 540
gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat 600
ttcatttgga gaggaaccat cttccacaca ctcaagccac actattggag aacacacagg 660
gacaacacac cataa 675
<210> 165
<211> 804
<212> DNA
<213> Zea mays
<400> 165
accgtcttcg gtacgcgctc actccgccct ctgcctttgt tactgccacg tttctctgaa 60
tgctctcttg tgtggtgatt gctgagagtg gtttagctgg atctagaatt acactctgaa 120
atcgtgttct gcctgtgctg attacttgcc gtcctttgta gcagcaaaat atagggacat 180
ggtagtacga aacgaagata gaacctacac agcaatacga gaaatgtgta atttggtgct 240
tagcggtatt tatttaagca catgttggtg ttatagggca cttggattca gaagtttgct 300
gttaatttag gcacaggctt catactacat gggtcaatag tatagggatt catattatag 360
gcgatactat aataatttgt tcgtctgcag agcttattat ttgccaaaat tagatattcc 420
tattctgttt ttgtttgtgt gctgttaaat tgttaacgcc tgaaggaata aatataaatg 480
acgaaatttt gatgtttatc tctgctcctt tattgtgacc ataagtcaag atcagatgca 540
cttgttttaa atattgttgt ctgaagaaat aagtactgac agtattttga tgcattgatc 600
tgcttgtttg ttgtaacaaa atttaaaaat aaagagtttc ctttttgttg ctctccttac 660
ctcctgatgg tatctagtat ctaccaactg acactatatt gcttctcttt acatacgtat 720
cttgctcgat gccttctccc tagtgttgac cagtgttact cacatagtct ttgctcattt 780
cattgtaatg cagataccaa gcgg 804
<210> 166
<211> 623
<212> DNA
<213> Cauliflower mosaic virus
<400> 166
ggtccgatgt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg 60
cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg 120
ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa 180
agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc 240
aaagcaagtg gattgatgtg atggtccgat gtgagacttt tcaacaaagg gtaatatccg 300
gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa 360
aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg 420
cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag 480
aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa 540
gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat 600
ttcatttgga gaggacacgc tga 623
<210> 167
<211> 8
<212> DNA
<213> Cauliflower mosaic virus
<400> 167
acacgctg 8
<210> 168
<211> 1790
<212> DNA
<213> Coix lacryma-jobi
<400> 168
gtgatgttca agatattgta atggtgttta ttttctatca aatagccata aaatgatata 60
caaaatgtta ttcatgattg atcctagtta cattcaaagt attaaatagc ttgcagatag 120
taaatagaca gtcattgtat aacctgtttt tttgactgtc tatgttcagt tccaagaact 180
tacagacaag aggttatgtg tagattgaac gtgcccttga cggcatccaa ctagcgaacc 240
acgagggaag cagatggtgg ccgttgaggg gctgttgacg caaagcatct ctctcggctg 300
ctctcgaaag ctccattgcg ggtggcggtc tggtggcacc aggaaattgc gtgagccaag 360
gcgggctcgt ctcggtctca caacacggca cgaaaccgtc acggcacacg gcaccaggat 420
ttccttcccc tcccctgccg ttctcctcat cataaatagc caccccctcc tcgcctcttt 480
tccccaactc atctgttctt cgtctcacac agccagatcc caatccctct cctcgcgaac 540
ttcgtcgatc tcccttccct cgcctcgctt caaggtacgg cgatcatcct cccgctttcc 600
ctcctcctcc tctagatgta gtacggagta cttgccatca tgcatcatgc tacatcacgc 660
tcgtgcgagc tctgggtcct cgatctggga acggaactgt gggatgctgc tcgtgcgatt 720
tattattggg gatctgggtt ctcgatctgg gaacggaact gtgggatgct gctcgtgcga 780
tttattattg gggatctggg ttctcgatct gggaacggaa ctgtgggatg cttgtaggca 840
ggtcggagat gggtcggatc gttgcttagg gttcgatctg ctcgtggttt tcttttaatc 900
cctgatgcat gatttatcgg tcatcctatt agatggaacc agtagggtga ctctgatccg 960
atatacttaa cctcgatctg gttcgatgtt cctggctagg cttgtgcgtc tgtttcgtca 1020
gaccagtttt gctgtttttg gtatggttgt gatgcccgtc caaatatgac taagcgagtg 1080
tagaatcatt ttatgaacta actgctggtc ttattaaatc tagatctgca tacgttgatg 1140
tactacgttc atagttgata cagtatgtat gaactagttg ctggtcgtat taattttgga 1200
tctgcatgtg tggtagcata taatgttcat aatacaattg atacagtatg atgtatgaac 1260
tatctgctgg tttattaaat ttggatctgc ttgtggtaaa aaatatgttt tttatatagt 1320
taccatgatg gattaatcta tacttctgat gtatatgctg cagttttctg ctgaggctgt 1380
agttttttcc agattaaaat acagcatgca tatttgctaa gctctgggcg tgtgaacgcc 1440
caccatggca ttgtccagta atagtaatga atttttttgt ttgcctgatg tgggagaaaa 1500
cacgcattgt ccagttattt tgttccatat gcattgtcct gttttgttgg atatgcatgc 1560
ttagaaaaca tatgcagcca ctgtttgata atgctttagc atctgcctgt tgaacatgca 1620
tgatctacct atctttattt tgtatgtact tgggtagtgg catgttgcta gttttccttg 1680
attctgtggc gtctacatgt tgagcttgca tatatgtttg ttgtccttct tttcctcctt 1740
ggtctactgc tatatgctta cccttttgtt tggctaattt tcaggtgcag 1790
<210> 169
<211> 481
<212> DNA
<213> Coix lacryma-jobi
<400> 169
gtgatgttca agatattgta atggtgttta ttttctatca aatagccata aaatgatata 60
caaaatgtta ttcatgattg atcctagtta cattcaaagt attaaatagc ttgcagatag 120
taaatagaca gtcattgtat aacctgtttt tttgactgtc tatgttcagt tccaagaact 180
tacagacaag aggttatgtg tagattgaac gtgcccttga cggcatccaa ctagcgaacc 240
acgagggaag cagatggtgg ccgttgaggg gctgttgacg caaagcatct ctctcggctg 300
ctctcgaaag ctccattgcg ggtggcggtc tggtggcacc aggaaattgc gtgagccaag 360
gcgggctcgt ctcggtctca caacacggca cgaaaccgtc acggcacacg gcaccaggat 420
ttccttcccc tcccctgccg ttctcctcat cataaatagc caccccctcc tcgcctcttt 480
t 481
<210> 170
<211> 93
<212> DNA
<213> Coix lacryma-jobi
<400> 170
ccccaactca tctgttcttc gtctcacaca gccagatccc aatccctctc ctcgcgaact 60
tcgtcgatct cccttccctc gcctcgcttc aag 93
<210> 171
<211> 1216
<212> DNA
<213> Coix lacryma-jobi
<400> 171
gtacggcgat catcctcccg ctttccctcc tcctcctcta gatgtagtac ggagtacttg 60
ccatcatgca tcatgctaca tcacgctcgt gcgagctctg ggtcctcgat ctgggaacgg 120
aactgtggga tgctgctcgt gcgatttatt attggggatc tgggttctcg atctgggaac 180
ggaactgtgg gatgctgctc gtgcgattta ttattgggga tctgggttct cgatctggga 240
acggaactgt gggatgcttg taggcaggtc ggagatgggt cggatcgttg cttagggttc 300
gatctgctcg tggttttctt ttaatccctg atgcatgatt tatcggtcat cctattagat 360
ggaaccagta gggtgactct gatccgatat acttaacctc gatctggttc gatgttcctg 420
gctaggcttg tgcgtctgtt tcgtcagacc agttttgctg tttttggtat ggttgtgatg 480
cccgtccaaa tatgactaag cgagtgtaga atcattttat gaactaactg ctggtcttat 540
taaatctaga tctgcatacg ttgatgtact acgttcatag ttgatacagt atgtatgaac 600
tagttgctgg tcgtattaat tttggatctg catgtgtggt agcatataat gttcataata 660
caattgatac agtatgatgt atgaactatc tgctggttta ttaaatttgg atctgcttgt 720
ggtaaaaaat atgtttttta tatagttacc atgatggatt aatctatact tctgatgtat 780
atgctgcagt tttctgctga ggctgtagtt ttttccagat taaaatacag catgcatatt 840
tgctaagctc tgggcgtgtg aacgcccacc atggcattgt ccagtaatag taatgaattt 900
ttttgtttgc ctgatgtggg agaaaacacg cattgtccag ttattttgtt ccatatgcat 960
tgtcctgttt tgttggatat gcatgcttag aaaacatatg cagccactgt ttgataatgc 1020
tttagcatct gcctgttgaa catgcatgat ctacctatct ttattttgta tgtacttggg 1080
tagtggcatg ttgctagttt tccttgattc tgtggcgtct acatgttgag cttgcatata 1140
tgtttgttgt ccttcttttc ctccttggtc tactgctata tgcttaccct tttgtttggc 1200
taattttcag gtgcag 1216
Claims (16)
- 재조합 DNA 분자로서,
a) 서열번호 56에 대해서 적어도 95 퍼센트(%)의 서열 동일성을 갖고, 프로모터 활성을 갖는 DNA 서열;
b) 서열번호 55 또는 56을 포함하는 DNA 서열; 및
c) 서열번호 56의 적어도 200개의 연속적인 뉴클레오타이드를 포함하는 단편으로서, 프로모터 활성을 지니는, 상기 단편
으로 이루어진 군으로부터 선택된 DNA 서열을 포함하되;
상기 DNA 서열은 이종성 전사 가능 DNA 분자에 작동 가능하게 연결되는, 재조합 DNA 분자. - 제1항에 있어서, 상기 DNA 서열은 서열번호 56의 DNA 서열에 대해서 적어도 97%의 서열 동일성을 갖는, 재조합 DNA 분자.
- 제1항에 있어서, 상기 DNA 서열은 서열번호 56의 DNA 서열에 대해서 적어도 99%의 서열 동일성을 갖는, 재조합 DNA 분자.
- 제1항에 있어서, 상기 이종성 전사 가능 DNA 분자는 관심대상 작물학적 유전자(gene of agronomic interest)인, 재조합 DNA 분자.
- 제4항에 있어서, 상기 관심대상 작물학적 유전자는 식물에서 제초제 내성(herbicide tolerance)을 부여하는, 재조합 DNA 분자.
- 제4항에 있어서, 상기 관심대상 작물학적 유전자는 식물에서 내충성(pest resistance)을 부여하는, 재조합 DNA 분자.
- 제1항의 재조합 DNA 분자를 포함하는 작제물(construct).
- 유전자전이 식물 세포(transgenic plant cell)로서,
a) 서열번호 56에 대해서 적어도 95%의 서열 동일성을 갖고, 프로모터 활성을 갖는 DNA 서열;
b) 서열번호 55 또는 56을 포함하는 DNA 서열;및
c) 서열번호 56의 적어도 200개의 연속적인 뉴클레오타이드를 포함하는 단편으로서, 프로모터 활성을 지니는, 상기 단편
으로 이루어진 군으로부터 선택된 DNA 서열을 포함하는 재조합 DNA 분자를 포함하되;
상기 DNA 서열은 이종성 전사 가능 DNA 분자에 작동 가능하게 연결되는, 유전자전이 식물 세포. - 제8항에 있어서, 상기 유전자전이 식물 세포는 외떡잎 식물 세포인, 유전자전이 식물 세포.
- 제8항에 있어서, 상기 유전자전이 식물 세포는 쌍떡잎 식물 세포인, 유전자전이 식물 세포.
- 유전자전이 식물로서,
a) 서열번호 56에 대해서 적어도 95%의 서열 동일성을 갖고, 프로모터 활성을 갖는 DNA 서열;
b) 서열번호 55 또는 56을 포함하는 DNA 서열; 및
c) 서열번호 56의 적어도 200개의 연속적인 뉴클레오타이드를 포함하는 단편으로서, 프로모터 활성을 지니는, 상기 단편
으로 이루어진 군으로부터 선택된 DNA 서열을 포함하는 재조합 DNA 분자를 포함하되;
상기 DNA 서열은 이종성 전사 가능 DNA 분자에 작동 가능하게 연결되는, 유전자전이 식물. - 제11항에 따른 유전자전이 식물의 부분.
- 제11항의 유전자전이 식물의 자손 식물(progeny plant)로서, 상기 자손 식물은 상기 재조합 DNA 분자를 포함하는, 유전자전이 식물의 자손 식물.
- 제11항의 유전자전이 식물의 유전자전이 종자로서, 상기 종자는 상기 재조합 DNA 분자를 포함하는, 유전자전이 종자.
- 전사 가능 DNA 분자를 발현시키는 방법으로서, 제11항에 따른 유전자전이 식물을 얻는 단계 및 상기 식물을 재배하는 단계를 포함하되, 상기 식물에서 상기 전사 가능 DNA 분자가 발현되는, 전사 가능 DNA 분자를 발현시키는 방법.
- 유전자전이 식물을 생산하는 방법으로서,
a) 식물 세포를 제1항의 재조합 DNA 분자로 형질전환(transforming)시켜 형질전환된 식물 세포를 생산하는 단계; 및
b) 상기 형질전환된 식물 세포로부터 유전자전이 식물을 재생시키는 단계를 포함하는, 유전자전이 식물을 생산하는 방법.
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| PCT/US2013/075813 WO2014100009A2 (en) | 2012-12-19 | 2013-12-17 | Plant regulatory elements and uses thereof |
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| KR1020207026181A KR102273360B1 (ko) | 2012-12-19 | 2013-12-17 | 식물 조절 요소 및 그의 용도 |
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| JP7049919B2 (ja) | 2018-05-29 | 2022-04-07 | 日本光電工業株式会社 | 固定装置および人工呼吸器 |
| JP7107756B2 (ja) | 2018-06-05 | 2022-07-27 | ポリプラ・エボニック株式会社 | シート及びシートの製造方法 |
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| WO2012134921A2 (en) * | 2011-03-25 | 2012-10-04 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
| WO2012158535A1 (en) * | 2011-05-13 | 2012-11-22 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
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