RU2018126740A - Пестицидные гены и способы использования - Google Patents
Пестицидные гены и способы использования Download PDFInfo
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- 230000000361 pesticidal effect Effects 0.000 title claims 11
- 238000000034 method Methods 0.000 title claims 5
- 108090000623 proteins and genes Proteins 0.000 title claims 2
- 229920001184 polypeptide Polymers 0.000 claims 22
- 102000004196 processed proteins & peptides Human genes 0.000 claims 22
- 108090000765 processed proteins & peptides Proteins 0.000 claims 22
- 125000003275 alpha amino acid group Chemical group 0.000 claims 19
- 239000002773 nucleotide Substances 0.000 claims 14
- 125000003729 nucleotide group Chemical group 0.000 claims 14
- 108020004707 nucleic acids Proteins 0.000 claims 11
- 102000039446 nucleic acids Human genes 0.000 claims 11
- 150000007523 nucleic acids Chemical class 0.000 claims 11
- 239000000203 mixture Substances 0.000 claims 7
- 241000607479 Yersinia pestis Species 0.000 claims 6
- 108020004414 DNA Proteins 0.000 claims 5
- 241000894006 Bacteria Species 0.000 claims 1
- 241000254173 Coleoptera Species 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 239000000084 colloidal system Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 239000000428 dust Substances 0.000 claims 1
- 239000000839 emulsion Substances 0.000 claims 1
- 239000008187 granular material Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000008188 pellet Substances 0.000 claims 1
- 239000000843 powder Substances 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 230000009261 transgenic effect Effects 0.000 claims 1
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Claims (34)
1. Рекомбинантный полипептид, обладающий пестицидной активностью, который содержит:
(a) полипептид, содержащий аминокислотную последовательность, обладающую по меньшей мере процентом идентичности последовательностей 90% с аминокислотной последовательностью, выбранной из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 58, 211, 99, 100, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 212, 213, 214, 215, 216, 217 или 218; или
(b) полипептид, содержащий аминокислотную последовательность, приведенную в SEQ ID NO: 58, 211, 99, 100, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 212, 213, 214, 215, 216, 217 или 218.
2. Полипептид по п. 1, который дополнительно содержит гетерологичную аминокислотную последовательность.
3. Композиция, содержащая полипептид по п. 1 или 2.
4. Рекомбинантная молекула нуклеиновой кислоты, кодирующая аминокислотную последовательность, содержащую:
(a) по меньшей мере 90% процент идентичности последовательностей с аминокислотной последовательностью, выбранной из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 58, 211, 99, 100, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 212, 213, 214, 215, 216, 217 или 218, где указанная аминокислотная последовательность кодирует полипептид, обладающий пестицидной активностью; или
(b) аминокислотную последовательность, приведенную в SEQ ID NO: 58, 211, 99, 100, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 212, 213, 214, 215, 216, 217 или 218;
где указанная рекомбинантная молекула нуклеиновой кислоты не является встречаемой в природе последовательностью, кодирующей указанный полипептид.
5. Рекомбинантная молекула нуклеиновой кислоты по п. 4, где указанная молекула нуклеиновой кислоты представляет собой синтетическу последовательность, разработанную для экспрессии в растении.
6. Рекомбинантная молекула нуклеиновой кислоты по п. 4 или 5, где указанная молекула нуклеиновой кислоты функционально связана с промотором, способным управлять экспрессией в клетке растения.
7. Рекомбинантная молекула нуклеиновой кислоты по п. 4, где указанная молекула нуклеиновой кислоты функционально связана с промотором, способным управлять экспрессией в бактериях.
8. Клетка-хозяин, содержащая рекомбинантную молекулу нуклеиновой кислоты по любому одному из пп. 4-7.
9. Клетка-хозяин по п. 8, где указанная клетка-хозяин представляет собой бактериальную клетку-хозяина.
10. ДНК конструкция, содержащая промотор, управляющий экспрессией в клетке растения, который функционально связан с рекомбинантной молекулой нуклеиновой кислоты, содержащей нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность, обладающую по меньшей мере процентом идентичности последовательностей 90% с аминокислотной последовательностью, выбранной из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 58, 211, 99, 100, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 212, 213, 214, 215, 216, 217 или 218, где указанный полипептид обладает пестицидной активностью.
11. ДНК конструкция по п. 10, где указанная нуклеотидная последовательность представляет собой синтетическую ДНК последовательность, разработанную для экспрессии в растении.
12. Вектор, содержащий ДНК конструкцию по п. 10 или 11.
13. Клетка-хозяин, содержащая вектор по п. 12.
14. Композиция, содержащая клетку-хозяина по п. 13.
15. Композиция по п. 14, где указанную композицию выбирают из группы, состоящей из порошка, пыли, пеллета, гранулы, спрея, эмульсии, коллоида и раствора.
16. Композиция по п. 14 или 15, где указанная композиция содержит приблизительно от 1% приблизительно до 99% по массе указанного полипептида.
17. Способ контроля популяции вредителя, включающий приведение в контакт указанной популяции вредителя с пестицидно-эффективным количеством композиции по п. 3.
18. Способ получения полипептида с пестицидной активностью, который включает культивирование клетки-хозяина по п. 13 в условиях, в которых экспрессируют молекулу нуклеиновой кислоты, кодирующую полипептид.
19. Растение, имеющее стабильно встроенную в его геном ДНК конструкцию, содержащую нуклеотидную последовательность, которая кодирует белок, обладающий пестицидной активностью, где указанная нуклеотидная последовательность содержит:
(a) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность из любой одной из SEQ ID NO: 58, 211, 99, 100, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 212, 213, 214, 215, 216, 217 или 218; или
(b) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность, обладающую по меньшей мере процентом идентичности последовательностей 90% с аминокислотной последовательностью, выбранной из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 58, 211, 99, 100, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 212, 213, 214, 215, 216, 217 или 218.
20. Трансгенное семя растения по п. 19.
21. Способ защиты растения от насекомого вредителя, который включает экспрессию в растении или его клетке нуклеотидной последовательности, которая кодирует пестицидный полипептид, где указанная нуклеотидная последовательность содержит:
(a) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность из любой одной из SEQ ID NO: 58, 211, 99, 100, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 212, 213, 214, 215, 216, 217 или 218; или
(b) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность, обладающую по меньшей мере процентом идентичности последовательностей 90% с аминокислотной последовательностью, выбранной из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 58, 211, 99, 100, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 212, 213, 214, 215, 216, 217 или 218, где указанный полипептид обладает пестицидной активностью.
22. Способ по п. 21, где указанное растение продуцирует пестицидный полипептид, обладающий пестицидной активностью против по меньшей мере одного из чешуекрылого вредителя, жесткокрылого вредителя или полужесткокрылого вредителя.
23. Способ увеличения урожая у растения, включающий выращивание в поле растения или его семени, обладающего стабильно встроенной в его геном ДНК конструкцией, содержащей промотор, управляющий экспрессией в растении, который функционально связан с нуклеотидной последовательностью, которая кодирует пестицидный полипептид, где указанная нуклеотидная последовательность содержит:
(a) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность из любой одной из SEQ ID NO: 58, 211, 99, 100, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 212, 213, 214, 215, 216, 217 или 218; или
(b) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность, обладающую по меньшей мере процентом идентичности последовательностей 90% с аминокислотной последовательностью, выбранной из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 58, 211, 99, 100, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 212, 213, 214, 215, 216, 217 или 218.
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2016
- 2016-12-16 EP EP16843278.9A patent/EP3394269A2/en not_active Withdrawn
- 2016-12-16 MA MA044159A patent/MA44159A/fr unknown
- 2016-12-16 CN CN202210367092.1A patent/CN114621330A/zh active Pending
- 2016-12-16 KR KR1020187020658A patent/KR20180091096A/ko active Search and Examination
- 2016-12-16 CA CA3008030A patent/CA3008030A1/en active Pending
- 2016-12-16 RU RU2021110857A patent/RU2021110857A/ru unknown
- 2016-12-16 US US15/530,267 patent/US10358654B2/en active Active
- 2016-12-16 BR BR112018012893-7A patent/BR112018012893A2/pt unknown
- 2016-12-16 MX MX2018007534A patent/MX2018007534A/es unknown
- 2016-12-16 UA UAA201808058A patent/UA126850C2/uk unknown
- 2016-12-16 CA CA3222614A patent/CA3222614A1/en active Pending
- 2016-12-16 AU AU2016379159A patent/AU2016379159A1/en not_active Abandoned
- 2016-12-16 MX MX2022003946A patent/MX2022003946A/es unknown
- 2016-12-16 EP EP23196415.6A patent/EP4278893A3/en active Pending
- 2016-12-16 CN CN201680080838.3A patent/CN109312354A/zh active Pending
- 2016-12-16 RU RU2018126740A patent/RU2746927C2/ru active
- 2016-12-16 WO PCT/US2016/067146 patent/WO2017112538A2/en active Application Filing
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2018
- 2018-06-21 PH PH12018501338A patent/PH12018501338A1/en unknown
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2019
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2020
- 2020-05-08 CL CL2020001235A patent/CL2020001235A1/es unknown
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2021
- 2021-08-10 US US17/398,575 patent/US11898153B2/en active Active
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2023
- 2023-09-13 US US18/466,111 patent/US20240043865A1/en active Pending
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RU2018126740A3 (ru) | 2020-05-15 |
EP3394269A2 (en) | 2018-10-31 |
CN114621330A (zh) | 2022-06-14 |
CL2019002973A1 (es) | 2020-01-24 |
US20220411814A1 (en) | 2022-12-29 |
UA126850C2 (uk) | 2023-02-15 |
PH12018501338A1 (en) | 2019-02-18 |
EP4278893A3 (en) | 2024-06-05 |
US20240043865A1 (en) | 2024-02-08 |
EP4278893A2 (en) | 2023-11-22 |
AU2016379159A1 (en) | 2018-06-28 |
CL2020001235A1 (es) | 2020-09-11 |
US11898153B2 (en) | 2024-02-13 |
US10358654B2 (en) | 2019-07-23 |
MA44159A (fr) | 2018-10-31 |
BR112018012893A2 (pt) | 2018-12-04 |
CA3222614A1 (en) | 2017-06-29 |
MX2018007534A (es) | 2018-09-07 |
CN109312354A (zh) | 2019-02-05 |
RU2021110857A (ru) | 2021-04-26 |
MX2022003946A (es) | 2022-04-25 |
RU2746927C2 (ru) | 2021-04-22 |
WO2017112538A2 (en) | 2017-06-29 |
US11118190B2 (en) | 2021-09-14 |
US20190292560A1 (en) | 2019-09-26 |
CL2018001708A1 (es) | 2018-10-12 |
CA3008030A1 (en) | 2017-06-29 |
KR20180091096A (ko) | 2018-08-14 |
US20170175134A1 (en) | 2017-06-22 |
WO2017112538A3 (en) | 2017-09-14 |
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