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BRPI0808715A8 - Preparação e uso de explantes de embrião de planta para transformação - Google Patents

Preparação e uso de explantes de embrião de planta para transformação

Info

Publication number
BRPI0808715A8
BRPI0808715A8 BRPI0808715A BRPI0808715A BRPI0808715A8 BR PI0808715 A8 BRPI0808715 A8 BR PI0808715A8 BR PI0808715 A BRPI0808715 A BR PI0808715A BR PI0808715 A BRPI0808715 A BR PI0808715A BR PI0808715 A8 BRPI0808715 A8 BR PI0808715A8
Authority
BR
Brazil
Prior art keywords
preparation
transformation
tissue
plant embryo
embryo explants
Prior art date
Application number
BRPI0808715A
Other languages
English (en)
Inventor
Jo Calabotta Beth
Lee Deppermann Kevin
D Dersch Erik
D Heise Jerald
Ranae Koestel Angela
L Ludwig Cindy
J Martinell Brian
Williams Edward
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39561923&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0808715(A8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of BRPI0808715A2 publication Critical patent/BRPI0808715A2/pt
Publication of BRPI0808715A8 publication Critical patent/BRPI0808715A8/pt

Links

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/003—Cutting apparatus specially adapted for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
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    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
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    • C12N9/10—Transferases (2.)
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    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0098—Plants or trees
    • A—HUMAN NECESSITIES
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    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
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    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
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  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

PREPARAÇÃO E USO DE EXPLANTES DE EMBRIÃO DE PLANTA PARA TRANSFORMAÇÃO. A presente invenção refere-se à excisão de material de explante compreendendo tecido meristemático a partir de sementes e armazenamento de tal material antes do uso subsequente em cultura de tecido de planta e transformação genética. Métodos para preparação, armazenamento e transformação de tecido são revelados, bem como tecido de meristema transformável produzido através de tais métodos e aparelhos para preparação de tecido.
BRPI0808715A 2007-03-09 2008-03-10 Preparação e uso de explantes de embrião de planta para transformação BRPI0808715A8 (pt)

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PCT/US2008/056400 WO2008112628A2 (en) 2007-03-09 2008-03-10 Preparation and use of plant embryo explants for transformation

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BRPI0808665-6A BRPI0808665B1 (pt) 2007-03-09 2008-03-10 Métodos e construções para produção de plantas de soja, algodão ou canola transgênicas usando seleção de espectinomicina
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Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
US7560611B2 (en) 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
BRPI0808715A8 (pt) * 2007-03-09 2017-04-18 Monsanto Technology Llc Preparação e uso de explantes de embrião de planta para transformação
WO2009114537A1 (en) 2008-03-10 2009-09-17 Monsanto Technology Llc Chimeric promoter molecules for gene expression in prokaryotes
BR122018017045B1 (pt) 2008-05-23 2019-10-22 Syngenta Participations Ag método para identificar embriões haplóides
WO2010002984A1 (en) 2008-07-01 2010-01-07 Monsanto Technology, Llc Recombinant dna constructs and methods for modulating expression of a target gene
US8153864B2 (en) * 2008-08-11 2012-04-10 Dow Agrosciences Llc Method for seed devitalization
US9873888B2 (en) 2009-10-23 2018-01-23 Monsanto Technology Llc Transgenic soybean plants and chromosomes
WO2011082107A1 (en) 2009-12-31 2011-07-07 Pioneer Hi-Bred International, Inc. Direct and continuous root alone or root/shoot production from transgenic events derived from green regenerative tissues and its applications
US20110162112A1 (en) * 2009-12-31 2011-06-30 Pioneer Hi-Bred International, Inc. Increasing time-efficiency of high-throughput transformation processes
US8207419B2 (en) * 2010-03-10 2012-06-26 Monsanto Technology Llc Cotton variety 09R798B2R2
WO2012021494A1 (en) * 2010-08-11 2012-02-16 Board Of Trustees Of Michigan State University Method for gene delivery into grain legumes and in vitro regeneration of same
JP5932832B2 (ja) 2010-12-17 2016-06-08 モンサント テクノロジー エルエルシー 植物細胞の適格性を改善する方法
ES2637862T3 (es) 2011-03-25 2017-10-17 Monsanto Technology Llc Elementos reguladores de plantas y usos de los mismos
US8735661B2 (en) 2011-03-30 2014-05-27 Monsanto Technology Llc Cotton transgenic event MON 88701 and methods of use thereof
WO2012168124A1 (en) 2011-06-06 2012-12-13 Bayer Cropscience Nv Methods and means to modify a plant genome at a preselected site
US9630472B2 (en) * 2011-09-23 2017-04-25 LSP Trucking, LLC System and method of ventilated transport
US9138750B2 (en) * 2011-12-29 2015-09-22 Weyerhaeuser Nr Company Spray apparatus and method for separating plant embryos
EP2827701B1 (en) 2012-03-19 2020-11-18 Malaysian Palm Oil Board Gene controlling shell phenotype in palm
US11807846B2 (en) * 2012-04-29 2023-11-07 Monsanto Technology Llc Methods for transforming corn explants
US20150307894A1 (en) 2012-11-28 2015-10-29 Monsanto Technology Llc Transgenic Plants With Enhanced Traits
JP2016502853A (ja) * 2012-12-19 2016-02-01 ダウ アグロサイエンシィズ エルエルシー 効率的なハイスループットトランスジェニック事象生成のための改善されたダイズ形質転換
CA2895184C (en) 2012-12-19 2021-11-23 Monsanto Technology Llc Plant regulatory elements and uses thereof
WO2014159632A1 (en) 2013-03-14 2014-10-02 Monsanto Technology Llc Plant regulatory elements and uses thereof
BR112015031127A2 (pt) 2013-06-14 2017-12-12 Monsanto Technology Llc evento de soja transgênica mon87751 e métodos para sua detecção e utilização
CN105792642A (zh) 2013-10-04 2016-07-20 美国陶氏益农公司 大豆转化方法
US20160237447A1 (en) 2013-10-07 2016-08-18 Monsanto Technology Llc Transgenic Plants With Enhanced Traits
US11713465B2 (en) * 2013-11-21 2023-08-01 Monsanto Technology, Llc Methods for transforming wheat explants and compositions therefor
WO2015134970A1 (en) * 2014-03-07 2015-09-11 Pioneer Hi-Bred International, Inc. Methods and systems for extracting dicot embryos
BR112016020670B1 (pt) * 2014-03-07 2023-02-07 E.I. Du Pont De Nemours And Company Método de extração de um embrião, sistema para a extração de um embrião
UA124487C2 (uk) 2014-03-20 2021-09-29 Монсанто Текнолоджі Елелсі Рекомбінантна молекула днк, яка надає рослині кукурудзи стійкості до глюфосинату і дикамба, та спосіб її використання
CN106471008B (zh) 2014-05-02 2021-04-09 马来西亚棕榈油协会 棕榈Mantle表型检测
KR20170007294A (ko) 2014-05-06 2017-01-18 다우 아그로사이언시즈 엘엘씨 종자를 촬상 및 배향시키기 위한 시스템 및 사용 방법
TW201543999A (zh) 2014-05-06 2015-12-01 Dow Agrosciences Llc 用於種子製備之系統及使用方法
US10487123B2 (en) 2014-10-16 2019-11-26 Monsanto Technology Llc Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests
BR122020004891B1 (pt) 2014-10-16 2024-01-30 Monsanto Technology Llc Molécula de ácido nucleico recombinante, polinucleotídeo, proteína inseticida quimérica, célula hospedeira, composição inibidora de insetos, e métodos para controlar uma praga de lepidópteros
JP6815322B2 (ja) * 2015-02-04 2021-01-20 モンサント テクノロジー エルエルシー 色素体形質転換方法
CN104785315A (zh) * 2015-04-01 2015-07-22 梁伟 种子脱粒器
CN109475124B (zh) 2015-08-27 2021-11-19 孟山都技术公司 新型昆虫抑制蛋白
JP2018527931A (ja) 2015-08-28 2018-09-27 パイオニア ハイ−ブレッド インターナショナル， インコーポレイテッド 植物のオクロバクテリウム（ｏｃｈｒｏｂａｃｔｒｕｍ）媒介形質転換
CN105713923A (zh) * 2016-03-14 2016-06-29 上海交通大学 一种植物愈伤组织遗传转化和植物组织培养方法
US11890750B2 (en) 2016-05-06 2024-02-06 Pioneer Hi-Bred International, Inc. Systems and methods for maize embryo plating and replating
US11266086B2 (en) * 2018-01-10 2022-03-08 Wisconsin Alumni Research Foundation Efficient plant transformation method
US11377662B2 (en) * 2018-01-10 2022-07-05 Wisconsin Alumni Research Foundation Agrobacterium-mediated and particle bombardment transformation method for cowpea and dry bean meristem explants
US12089608B2 (en) * 2018-02-09 2024-09-17 Healthy Truth Llc Method of preparing a food product
CN112166189A (zh) * 2018-05-24 2021-01-01 孟山都技术公司 植物基因组编辑
CN112203505A (zh) * 2018-05-24 2021-01-08 孟山都技术公司 植物基因组编辑
BR112020026640A2 (pt) * 2018-06-28 2021-04-06 Pioneer Hi-Bred International, Inc. Métodos para selecionar plantas transformadas
CN110129363A (zh) * 2019-06-11 2019-08-16 先正达作物保护股份公司 提高番茄CRISPR/Cas9基因编辑效率的方法
US12022787B2 (en) * 2019-06-19 2024-07-02 Wisconsin Alumni Research Foundation Efficient monocot embryo extraction and preservation methods and novel uses thereof
US11593919B2 (en) 2019-08-07 2023-02-28 Nanotronics Imaging, Inc. System, method and apparatus for macroscopic inspection of reflective specimens
US10915992B1 (en) 2019-08-07 2021-02-09 Nanotronics Imaging, Inc. System, method and apparatus for macroscopic inspection of reflective specimens
WO2021080598A1 (en) 2019-10-25 2021-04-29 Instrumentation Laboratory Company Biocide compositions compatible with enzyme biosensors and methods of use thereof
CN110656129A (zh) * 2019-11-05 2020-01-07 贵州大学 一种农杆菌介导的薏苡遗传转化方法
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
AR124445A1 (es) 2020-12-21 2023-03-29 Monsanto Technology Llc Proteínas inhibidoras de insectos novedosas
UY39585A (es) 2020-12-23 2022-07-29 Monsanto Technology Llc Proteínas que exhiben actividad inhibidora de insectos frente a plagas con importancia agrícola de plantas de cultivo y semillas
AU2021412979A1 (en) 2020-12-31 2023-07-06 Monsanto Technology Llc Novel insect inhibitory proteins
EP4402272A2 (en) 2021-09-17 2024-07-24 University of Freiburg Proteins for regulation of symbiotic infection and associated regulatory elements
US20230143932A1 (en) 2021-09-20 2023-05-11 University Of Freiburg Pin6 proteins for the formation of nodule-like structures
AU2022349096A1 (en) * 2021-09-27 2024-04-04 Monsanto Technology Llc Compositions and methods for transformation of monocot seed excised embryo explants
AR127148A1 (es) * 2021-09-27 2023-12-20 Monsanto Technology Llc Composiciones y métodos para la transformación de explantes de embriones extirpados de semillas de monocotiledóneas
WO2023141487A1 (en) * 2022-01-20 2023-07-27 Inari Agriculture Technology, Inc. Improved soybean explant preparation and transformation
US20240011045A1 (en) 2022-05-19 2024-01-11 Cambridge Enterprise Limited Proteins for regulation of symbiotic nodule organ identity
WO2023250352A1 (en) * 2022-06-23 2023-12-28 Monsanto Technology Llc Flotation of cultured embryo explants for improved plant regeneration efficiency
WO2024015983A1 (en) * 2022-07-15 2024-01-18 Monsanto Technology Llc Systems and methods for dry embryo explant purification
WO2024074888A2 (en) 2022-10-03 2024-04-11 The Regents Of The University Of California Circumventing barriers to hybrid crops from genetically distant crosses
GB2628406A (en) * 2023-03-23 2024-09-25 Green Bioactives Ltd Methods of using vascular plant stem cells

Family Cites Families (245)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3068142A (en) * 1962-12-11 Solvent system comprising dimethyl
US489A (en) 1837-11-25 Improvement in the mode of constructing springs for carriages, wagons
US894A (en) 1838-08-25 Machinery for raising heavy bodies
US5637A (en) 1848-06-20 Improvement in water-wheels
US5273A (en) 1847-09-04 George w
US550318A (en) * 1895-11-26 daniel
US1849786A (en) * 1929-09-11 1932-03-15 Victor G Bloede Company Process of treating seeds to remove the seed coats and to separate out the endosperms
GB402848A (en) 1932-06-14 1933-12-14 Eiji Satake Method of treating kaoliang
GB439399A (en) * 1935-03-29 1935-12-05 Robert Henry Brown Machine for shelling rice and other hard kernelled grains, seeds and beans
US2283449A (en) * 1939-04-15 1942-05-19 Meneux Charles Garlic separator
GB657644A (en) 1949-01-14 1951-09-26 Robert Cuthbertson Carter Improvements in or relating to apparatus for separating and/or extracting stalks and the like from vegetable tissues
GB861711A (en) 1958-03-18 1961-02-22 Istvan Kovasznay Improvement in the method for peeling and processing soya beans and apparatus therefor
US3104692A (en) 1962-02-26 1963-09-24 Floyd O Davis Safflower decorticator
US3301292A (en) * 1964-02-06 1967-01-31 Food Engineering International Sonic grain hulling apparatus and method
FR2067956A5 (pt) 1969-11-24 1971-08-20 Sepial
US3667523A (en) 1969-12-29 1972-06-06 Food Eng Intern Inc Apparatus and process for the removal of the germ and bran coat from cereal grains
SE387622B (sv) 1973-10-01 1976-09-13 Euroc Administration Ab Sett och anordning for vatformultning av mycket heterogent atminstone delvis biologiskt nedbrytbart avfallsmaterial
JPS51145756A (en) 1975-06-02 1976-12-14 Satake Eng Co Ltd Transmission of husker
JPS5930085B2 (ja) 1977-09-22 1984-07-25 旭松食品株式会社 高圧自動瞬間蒸煮器
US4220287A (en) 1978-03-23 1980-09-02 Maple Leaf Mills Limited Process for the treatment of oats
US4301183A (en) 1978-05-26 1981-11-17 Cereal Enterprises, Inc. Method and apparatus for degerminating a grain kernel by impelling the kernels along a guide vane into an impact surface
US5250313A (en) 1978-05-26 1993-10-05 Cereal Enterprises, Inc. Grain milling and degermination process
DE3025606A1 (de) 1980-07-05 1982-02-11 Bayer Ag, 5090 Leverkusen Schlagzaehe polyamid-formmassen
US4326358A (en) * 1980-07-17 1982-04-27 Agrigenetics Research Associates Limited Hybrids
IL64710A0 (en) 1982-01-05 1982-03-31 Israel Ministry Agriculture Apparatus for separating pomegranate seeds,scanning apparatus and techniques useful in connection therewith,and storage and packaging techniques for the separated seeds
JPS5982063A (ja) 1982-11-02 1984-05-11 Pelican:Kk 丸大豆を子葉と胚芽と皮に分離する方法
JPS5982063U (ja) 1982-11-24 1984-06-02 ダイワゴルフ株式会社 ゴルフクラブヘツド
US5094945A (en) 1983-01-05 1992-03-10 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
US4761373A (en) 1984-03-06 1988-08-02 Molecular Genetics, Inc. Herbicide resistance in plants
US4581847A (en) 1984-09-04 1986-04-15 Molecular Genetics Research And Development Tryptophan overproducer mutants of cereal crops
US6774283B1 (en) 1985-07-29 2004-08-10 Calgene Llc Molecular farming
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
WO1987004181A1 (en) 1986-01-08 1987-07-16 Rhone-Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the same
EP0242236B2 (en) 1986-03-11 1996-08-21 Plant Genetic Systems N.V. Plant cells resistant to glutamine synthetase inhibitors, made by genetic engineering
US5024944A (en) * 1986-08-04 1991-06-18 Lubrizol Genetics, Inc. Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species
US5637489A (en) 1986-08-23 1997-06-10 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5276268A (en) 1986-08-23 1994-01-04 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5273894A (en) 1986-08-23 1993-12-28 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5013659A (en) 1987-07-27 1991-05-07 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5378824A (en) 1986-08-26 1995-01-03 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5605011A (en) 1986-08-26 1997-02-25 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5003045A (en) * 1986-08-29 1991-03-26 Lubrizol Genetics, Inc. Modified 7S legume seed storage proteins
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5015580A (en) * 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5359142A (en) 1987-01-13 1994-10-25 Monsanto Company Method for enhanced expression of a protein
US5322938A (en) 1987-01-13 1994-06-21 Monsanto Company DNA sequence for enhancing the efficiency of transcription
CN87100603A (zh) 1987-01-21 1988-08-10 昂科公司 抗黑素瘤疫苗
JPS647107A (en) 1987-06-30 1989-01-11 Yaskawa Denki Seisakusho Kk Weaving device for industrial robot
JP2580248B2 (ja) 1987-07-07 1997-02-12 三井石油化学工業株式会社 培養装置
US5238835A (en) 1987-07-20 1993-08-24 University Of Guelph Process to induce desiccation tolerance in somatic embryos
US5229114A (en) 1987-08-20 1993-07-20 The United States Of America As Represented By The Secretary Of Agriculture Approaches useful for the control of root nodulation of leguminous plants
AU605690B2 (en) 1988-04-26 1991-01-17 Satake Engineering Co. Ltd. Process of and system for flouring grains
WO1989012102A1 (en) 1988-06-01 1989-12-14 The Texas A&M University System Method for transforming plants via the shoot apex
US5258300A (en) 1988-06-09 1993-11-02 Molecular Genetics Research And Development Limited Partnership Method of inducing lysine overproduction in plants
JPH0621042B2 (ja) 1988-06-21 1994-03-23 三愛石油株式会社 恒温槽用清浄剤
US5416011A (en) * 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
IT8833158V0 (it) 1988-09-01 1988-09-01 Schiavi Massimo Eufiber-fibrabuona
JPH0659776B2 (ja) 1988-09-29 1994-08-10 鬼怒川ゴム工業株式会社 分割式ウインドガラスのシール構造
US5597718A (en) 1988-10-04 1997-01-28 Agracetus Genetically engineering cotton plants for altered fiber
GB8825402D0 (en) 1988-10-31 1988-11-30 Cambridge Advanced Tech Sulfonamide resistance genes
IT1232380B (it) * 1989-01-25 1992-02-17 Ferro Sergio Macchina tritatrice di nocciole e mandorle tostate e di cioccolato inbarre
WO1990010076A1 (en) 1989-02-24 1990-09-07 Monsanto Company Synthetic plant genes and method for preparation
US5106739A (en) 1989-04-18 1992-04-21 Calgene, Inc. CaMv 355 enhanced mannopine synthase promoter and method for using same
US4986997A (en) * 1989-04-19 1991-01-22 Kansas State University Research Foundation Method of separating wheat germ from whole wheat
US5217902A (en) 1989-05-26 1993-06-08 Dna Plant Technology Corporation Method of introducing spectinomycin resistance into plants
US5073675A (en) * 1989-05-26 1991-12-17 Dna Plant Technology Corporation Method of introducing spectinomycin resistance into plants
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5689041A (en) 1989-08-10 1997-11-18 Plant Gentic Systems N.V. Plants modified with barstar for fertility restoration
US6051753A (en) 1989-09-07 2000-04-18 Calgene, Inc. Figwort mosaic virus promoter and uses
DK0426641T3 (da) 1989-10-31 2000-10-23 Monsanto Co Promotor til transgene planter
ATE199098T1 (de) * 1989-11-17 2001-02-15 Monsanto Co Gegen glutaminsynthetaseinhibitoren resistente sojabohnenpflanzen
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5837848A (en) 1990-03-16 1998-11-17 Zeneca Limited Root-specific promoter
US6426447B1 (en) 1990-11-14 2002-07-30 Monsanto Technology Llc Plant seed oils
US5543576A (en) 1990-03-23 1996-08-06 Mogen International Production of enzymes in seeds and their use
US5969214A (en) 1990-06-11 1999-10-19 Calgene, Inc. Glycogen biosynthetic enzymes in plants
RU2148081C1 (ru) 1990-06-18 2000-04-27 Монсанто Компани Способ получения генетически трансформированных растений с повышенным содержанием крахмала и рекомбинантная двухцепочечная днк-молекула
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
DE69132939T2 (de) 1990-06-25 2002-11-14 Monsanto Technology Llc Glyphosattolerante pflanzen
US6483008B1 (en) 1990-08-15 2002-11-19 Calgene Llc Methods for producing plants with elevated oleic acid content
US5639949A (en) * 1990-08-20 1997-06-17 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
WO1992003041A1 (en) * 1990-08-21 1992-03-05 Florigene Bv Method for producing transformed chrysanthemum plants
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
JPH06504668A (ja) 1990-12-26 1994-06-02 モンサント　カンパニー 果実の成熟および植物の老化の制御
US5767366A (en) 1991-02-19 1998-06-16 Louisiana State University Board Of Supervisors, A Governing Body Of Louisiana State University Agricultural And Mechanical College Mutant acetolactate synthase gene from Ararbidopsis thaliana for conferring imidazolinone resistance to crop plants
AU1663392A (en) 1991-03-06 1992-10-06 Agracetus, Inc. Particle mediated transformation of cotton
GB9105095D0 (en) 1991-03-11 1991-04-24 H & G Process Contracting Improved clean power generation
US5286635A (en) * 1991-09-24 1994-02-15 Freshworld, L.P. Genetically transformed pea plants and methods for their production
US5262316A (en) * 1991-11-22 1993-11-16 Dna Plant Technology Corporation Genetically transformed pepper plants and methods for their production
WO1994000977A1 (en) 1992-07-07 1994-01-20 Japan Tobacco Inc. Method of transforming monocotyledon
WO1994002620A2 (en) 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US6011199A (en) 1992-12-15 2000-01-04 Commonwealth Scientific Method for producing fruiting plants with improved fruit flavour
US6414222B1 (en) 1993-02-05 2002-07-02 Regents Of The University Of Minnesota Gene combinations for herbicide tolerance in corn
CH688849A5 (de) 1993-02-25 1998-04-30 Buehler Ag Ruehrwerksmuehle.
JP3759628B2 (ja) 1993-06-08 2006-03-29 麒麟麦酒株式会社 ブリーチング除草剤耐性植物の製造
US5362865A (en) 1993-09-02 1994-11-08 Monsanto Company Enhanced expression in plants using non-translated leader sequences
EP0672752B1 (en) 1993-09-03 2004-05-26 Japan Tobacco Inc. Method of transforming monocotyledon by using scutellum of immature embryo
JPH08509871A (ja) 1993-09-30 1996-10-22 アグラシータス インコーポレイテッド 異種ペルオキシダーゼを生産するトランスジェニック綿植物
ES2287933T3 (es) 1993-12-08 2007-12-16 Japan Tobacco Inc. Procedimiento de transformacion de plantas y vector para ello.
AU690999B2 (en) * 1994-06-10 1998-05-07 Dupont Nutrition Biosciences Aps Transformation of guar
US5939602A (en) 1995-06-06 1999-08-17 Novartis Finance Corporation DNA molecules encoding plant protoporphyrinogen oxidase and inhibitor-resistant mutants thereof
US6828475B1 (en) 1994-06-23 2004-12-07 Calgene Llc Nucleic acid sequences encoding a plant cytoplasmic protein involved in fatty acyl-CoA metabolism
US6080560A (en) 1994-07-25 2000-06-27 Monsanto Company Method for producing antibodies in plant cells
US6140075A (en) 1994-07-25 2000-10-31 Monsanto Company Method for producing antibodies and protein toxins in plant cells
US5750876A (en) 1994-07-28 1998-05-12 Monsanto Company Isoamylase gene, compositions containing it, and methods of using isoamylases
US5736369A (en) * 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5415085A (en) 1994-09-01 1995-05-16 Thomson; Kirk Apparatus for shelling and separating any type of nut or legume
WO1996010341A1 (en) 1994-10-03 1996-04-11 Schouten Industries B.V. Food and health products
US5633437A (en) 1994-10-11 1997-05-27 Sandoz Ltd. Gene exhibiting resistance to acetolactate synthase inhibitor herbicides
US5716837A (en) 1995-02-10 1998-02-10 Monsanto Company Expression of sucrose phosphorylase in plants
US5572827A (en) 1995-05-05 1996-11-12 Ball Horticultural Company Method for applying hydrogel coatings to embryonic plants
FR2734842B1 (fr) 1995-06-02 1998-02-27 Rhone Poulenc Agrochimie Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides
US20050158699A1 (en) * 1995-06-07 2005-07-21 Kadkade Prakash G. Cryopreservation of plant cells
US5965438A (en) * 1995-06-07 1999-10-12 Phyton, Inc. Cryopreservation of plant cells
US5846797A (en) * 1995-10-04 1998-12-08 Calgene, Inc. Cotton transformation
JPH09122510A (ja) 1995-11-02 1997-05-13 Satake Eng Co Ltd 脱ぷ装置
US5693512A (en) 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US6091002A (en) 1996-03-13 2000-07-18 Monsanto Company Polyhydroxyalkanoates of narrow molecular weight distribution prepared in transgenic plants
US6946588B2 (en) 1996-03-13 2005-09-20 Monsanto Technology Llc Nucleic acid encoding a modified threonine deaminase and methods of use
US5958745A (en) 1996-03-13 1999-09-28 Monsanto Company Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants
US6166292A (en) 1996-04-26 2000-12-26 Ajinomoto Co., Inc. Raffinose synthetase gene, method of producing raffinose and transgenic plant
US5985605A (en) 1996-06-14 1999-11-16 Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada DNA sequences encoding phytases of ruminal microorganisms
AU3585497A (en) 1996-06-27 1998-01-14 Weyerhaeuser Company Fluid switch
US5998700A (en) 1996-07-02 1999-12-07 The Board Of Trustees Of Southern Illinois University Plants containing a bacterial Gdha gene and methods of use thereof
AU3495297A (en) 1996-07-08 1998-02-02 Pioneer Hi-Bred International, Inc. Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
US5850019A (en) 1996-08-06 1998-12-15 University Of Kentucky Research Foundation Promoter (FLt) for the full-length transcript of peanut chlorotic streak caulimovirus (PCLSV) and expression of chimeric genes in plants
US5750848A (en) 1996-08-13 1998-05-12 Monsanto Company DNA sequence useful for the production of polyhydroxyalkanoates
AU4208797A (en) 1996-09-05 1998-03-26 Unilever Plc Salt-inducible promoter derivable from a lactic acid bacterium, and its use in a lactic acid bacterium for production of a desired protein
EP0938573A2 (en) 1996-10-29 1999-09-01 Calgene LLC Plant cellulose synthase and promoter sequences
EP0946737A2 (en) 1996-11-07 1999-10-06 Zeneca Limited Herbicide resistant plants
JP2001508661A (ja) 1997-01-20 2001-07-03 プラント ジエネテイツク システムズ エヌ．ブイ 病原体誘導植物プロモーター
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
US5922564A (en) 1997-02-24 1999-07-13 Performance Plants, Inc. Phosphate-deficiency inducible promoter
US6040497A (en) 1997-04-03 2000-03-21 Dekalb Genetics Corporation Glyphosate resistant maize lines
US7022896B1 (en) 1997-04-04 2006-04-04 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
US7105724B2 (en) 1997-04-04 2006-09-12 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
US6171640B1 (en) 1997-04-04 2001-01-09 Monsanto Company High beta-conglycinin products and their use
JPH10276748A (ja) 1997-04-10 1998-10-20 Suzuyo Kogyo Kk 膨潤した種子の種皮及び胚軸除去機
US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
AR013633A1 (es) 1997-04-11 2001-01-10 Calgene Llc METODO PARA LA ALTERACIoN DE LA COMPOSICIoN DE ÁCIDOS GRASOS DE CADENA MEDIA EN SEMILLAS VEGETALES QUE EXPRESAN UNA TIOESTERASA QUE PREFIERE CADENA MEDIA VEGETAL HETERoLOGA.
US6380466B1 (en) 1997-05-08 2002-04-30 Calgene Llc Production of improved rapeseed exhibiting yellow-seed coat
KR20010013426A (ko) 1997-06-05 2001-02-26 칼진 엘엘시 지방 아실-ＣｏＡ: 지방 알코올 아실트란스페라제
US6140555A (en) 1997-06-06 2000-10-31 Mississippi State University Methods for maize transformation coupled with adventitious regeneration utilizing nodal section explants and mature zygotic embryos
WO1999002267A1 (de) * 1997-07-09 1999-01-21 Maschinen-Und Mühlenbau Wittenberg Gmbh Verfahren zum vermahlen von körnerfrüchten sowie vorrichtung zur durchführung des verfahrens
EP1002115B9 (en) 1997-08-07 2008-12-03 Auburn University Universal chloroplast integration and expression vectors and transformed plants
US5994624A (en) * 1997-10-20 1999-11-30 Cotton Incorporated In planta method for the production of transgenic plants
JPH11138024A (ja) * 1997-11-13 1999-05-25 Shizuoka Seiki Co Ltd 精穀機
IL122270A0 (en) 1997-11-20 1998-04-05 Yeda Res & Dev DNA molecules conferring to plants resistance to a herbicide and plants transformed thereby
US6072103A (en) 1997-11-21 2000-06-06 Calgene Llc Pathogen and stress-responsive promoter for gene expression
JPH11164678A (ja) 1997-12-05 1999-06-22 Suzuyo Kogyo Kk 浸漬大豆の子葉、胚軸、種皮分離装置
US6153813A (en) * 1997-12-11 2000-11-28 Mississippi State University Methods for genotype-independent nuclear and plastid transformation coupled with clonal regeneration utilizing mature zygotic embryos in rice (Oryza sativa) seeds
KR100245454B1 (ko) * 1997-12-16 2000-03-02 신명수 기계적 분리에 의한 고순도 배아 분리 방법
US6653530B1 (en) 1998-02-13 2003-11-25 Calgene Llc Methods for producing carotenoid compounds, tocopherol compounds, and specialty oils in plant seeds
EP1056862A1 (en) 1998-02-26 2000-12-06 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
DE69920879T2 (de) 1998-02-26 2005-10-13 Pioneer Hi-Bred International, Inc. Konstitutive maispromotoren
US5914451A (en) 1998-04-06 1999-06-22 Monsanto Company Efficiency soybean transformation protocol
US6635806B1 (en) 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
JP3743599B2 (ja) 1998-05-15 2006-02-08 株式会社サタケ 製粉方法及び該製粉方法による製粉システム
US6307123B1 (en) 1998-05-18 2001-10-23 Dekalb Genetics Corporation Methods and compositions for transgene identification
WO1999063096A2 (en) 1998-06-05 1999-12-09 Calgene Llc Acyl coa:cholesterol acyltransferase related nucleic acid sequences
ES2293726T3 (es) 1998-06-12 2008-03-16 Calgene Llc Acidos grasos poliinsaturados en plantas.
EP1098962B1 (en) 1998-07-02 2009-09-09 Calgene LLC Diacylglycerol acyl transferase proteins
BR9912019A (pt) 1998-07-10 2002-02-19 Calgene Llc Expressão de peptìdeos de eucarióticos em plastìdeos de plantas
JP2000083680A (ja) 1998-07-16 2000-03-28 Nippon Paper Industries Co Ltd 光誘導型プロモ―タ―の制御下に置かれた不定芽再分化遺伝子を選抜マ―カ―遺伝子とする植物への遺伝子導入方法及びこれに用いる植物への遺伝子導入用ベクタ―
US6476294B1 (en) 1998-07-24 2002-11-05 Calgene Llc Plant phosphatidic acid phosphatases
WO2000007430A2 (en) 1998-08-04 2000-02-17 Cargill, Incorporated Plant fatty acid desaturase promoters
US6365802B2 (en) 1998-08-14 2002-04-02 Calgene Llc Methods for increasing stearate content in soybean oil
AU764100B2 (en) 1998-10-01 2003-08-07 Pioneer Hi-Bred International, Inc. Method of plant transformation
US6531648B1 (en) 1998-12-17 2003-03-11 Syngenta Participations Ag Grain processing method and transgenic plants useful therein
CA2359868A1 (en) 1999-01-14 2000-07-20 Monsanto Company Soybean transformation method
AU777365B2 (en) * 1999-03-10 2004-10-14 Temasek Life Sciences Laboratory Limited Agrobacterium-mediated transformation of cotton with novel explants
DE60025713T2 (de) 1999-04-15 2006-11-02 Calgene Llc, Davis Nukleinsäuresequenzen für proteine, die an der tocopherolbiosynthese beteiligt sind
US6232526B1 (en) 1999-05-14 2001-05-15 Dekalb Genetics Corp. Maize A3 promoter and methods for use thereof
US6194636B1 (en) 1999-05-14 2001-02-27 Dekalb Genetics Corp. Maize RS324 promoter and methods for use thereof
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
US6207879B1 (en) 1999-05-14 2001-03-27 Dekalb Genetics Corporation Maize RS81 promoter and methods for use thereof
JP2003501065A (ja) 1999-06-08 2003-01-14 カルジーン エルエルシー 脂肪酸のβ酸化に関わる蛋白質をコードする核酸配列とその使用法
US6770465B1 (en) 1999-06-09 2004-08-03 Calgene Llc Engineering B-ketoacyl ACP synthase for novel substrate specificity
ATE449854T1 (de) 1999-06-11 2009-12-15 Temasek Life Sciences Lab Ltd Hocheffiziente, durch agrobakterium-vermittelte transformation von baumwoll-pflanzen unter verwendung von blattstielexplantaten
WO2001004314A2 (en) 1999-07-12 2001-01-18 Monsanto Technology, Llc. Nucleic acid molecules and proteins associated with sterol synthesis and metabolism
JP2001017107A (ja) 1999-07-12 2001-01-23 Perikan:Kk 丸大豆を子葉と胚芽と皮に分離する方法
EP1196024B1 (en) 1999-07-20 2005-10-05 GVS Gesellschaft für Erwerb und Verwertung von Schutzrechten MBH Novel method for the generation and selection of transgenic linseed/flax plants
US6603061B1 (en) * 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
JP2005055175A (ja) * 1999-09-07 2005-03-03 National Agriculture & Bio-Oriented Research Organization 試料調製方法および装置
WO2001029241A2 (en) * 1999-10-15 2001-04-26 Calgene Llc Methods and vectors for site-specific recombination in plant cell plastids
AU1997101A (en) 1999-10-25 2001-05-08 Sudwestdeutsche Saatzucht Plants with a modified flower and seed development
WO2001032032A1 (fr) 1999-10-28 2001-05-10 Ajinomoto Co., Inc. Matiere grasse/huile de germe de soja et procede de production d'un produit a base de soja a concentration elevee en germe
EP2339005A3 (en) 1999-12-16 2011-10-12 Monsanto Technology LLC Dna constructs for expression of heterologous polypeptides in plants
US6613963B1 (en) 2000-03-10 2003-09-02 Pioneer Hi-Bred International, Inc. Herbicide tolerant Brassica juncea and method of production
JP3461324B2 (ja) 2000-04-12 2003-10-27 株式会社ヤマダフーズ 大豆子実から子葉の分離方法
NL1015529C2 (nl) 2000-06-26 2002-01-08 Tissue Culture Propagation Int Werkwijze en inrichting voor het vermeerderen van weefseldelen.
JP2002019886A (ja) 2000-07-06 2002-01-23 Toshiba Electric Appliance Co Ltd 飲料混合装置および飲料提供装置
US6706950B2 (en) 2000-07-25 2004-03-16 Calgene Llc Nucleic acid sequences encoding β-ketoacyl-ACP synthase and uses thereof
US7279336B2 (en) * 2000-09-14 2007-10-09 Gelvin Stanton B Methods and compositions for enhanced plant cell transformation
JP2002119886A (ja) 2000-10-12 2002-04-23 Aqm Kyushu Technos Kk 分離方法及び分離装置
CA2425956C (en) 2000-10-30 2014-12-23 Maxygen, Inc. Novel glyphosate n-acetyltransferase (gat) genes
US7045164B2 (en) 2000-11-09 2006-05-16 Cargill, Incorporated Soybean processing
US7057089B2 (en) 2000-11-14 2006-06-06 Pioneer Hi-Bred International, Inc. Methods for transforming immature maize embryos
US6581535B2 (en) * 2000-12-07 2003-06-24 Kinze Manufacturing, Inc. Agricultural seed meter
AU2002245266A1 (en) 2001-01-16 2002-09-12 Monsanto Technology Llc Novel mutilple shoot proliferation and regeneration system for plants
KR100402513B1 (ko) 2001-02-16 2003-10-22 주식회사 싸이젠하베스트 유전자 조작을 통한 식물체의 효과적인 형질전환 방법
CN1149918C (zh) 2001-02-26 2004-05-19 山西省农业生物技术研究中心 农杆菌介导植物萌发种子基因转化方法
GB0107510D0 (en) * 2001-03-26 2001-05-16 Univ Bristol New elongase gene and a process for the production of -9-polyunsaturated fatty acids
US6422137B1 (en) 2001-06-14 2002-07-23 Mohammad Nakhei-Nejad Pistachio huller
EP2272965A3 (en) 2001-07-06 2011-01-19 Monsanto Technology LLC Methods for enhancing segregation of transgenes in plants and compositions thereof
CA2457479A1 (en) * 2001-08-24 2003-03-06 Basf Plant Science Gmbh In planta transformation by embryo imbibition of agrobacterium
WO2003040306A2 (en) 2001-11-07 2003-05-15 Genesis Research And Development Corporation Limited Compositions from the grasses lolium perenne and festuca arundinacea
US6936294B2 (en) 2001-12-04 2005-08-30 Satake Usa, Inc. Corn degermination process
US20050042305A1 (en) * 2002-01-31 2005-02-24 Yaeta Endo Germ extract for cell-free protein synthesis and process for producing the same
US6959617B2 (en) 2002-05-24 2005-11-01 Monsanto Technology Llc Seed coring system and method for arranging seed cores for analysis
JP2003339395A (ja) 2002-05-27 2003-12-02 Yaeta Endo 無細胞タンパク質合成用胚芽の選別方法および無細胞タンパク質合成用胚芽抽出物の製造方法
CN1668744A (zh) * 2002-06-22 2005-09-14 辛根塔参与股份公司 转化大豆的方法
US20040009601A1 (en) * 2002-07-15 2004-01-15 The Regents Of The University Of California Methods for the regeneration and transformation of cotton
US7615678B2 (en) 2002-07-18 2009-11-10 Monsanto Technology Llc Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
JP4079216B2 (ja) * 2002-08-05 2008-04-23 独立行政法人農業・食品産業技術総合研究機構 材料の保持、分析、選別装置、方法および選別物
US7141260B2 (en) 2002-08-29 2006-11-28 Pioneer Hi-Bred International, Inc. Apparatus and method for removal of seed pericarp
TW558420B (en) * 2002-09-19 2003-10-21 Ming-Yuan Chen Mechanism for removing and cutting open lotus nut for lotus nut processing machine
WO2004029256A2 (es) 2002-09-27 2004-04-08 Centro De Ingenieria Genetica Y Biotecnologia Vector para la producción de plantas angiospermas transplastómicas
FR2848064B1 (fr) * 2002-12-06 2006-01-13 Bayer Cropscience Sa Plantes legumineuses transplastomiques fertiles
BR0317579A (pt) 2002-12-18 2005-11-22 Athenix Corp Composições e métodos para conferir resistência a herbicidas em plantas, células de plantas, tecidos e sementes
EP1594961B1 (en) 2003-02-18 2013-12-25 Monsanto Technology LLC Glyphosate resistant class i 5-enolpyruvylshikimate-3-phosphate synthase (epsps)
US8841511B2 (en) 2003-03-03 2014-09-23 Rutgers, The State University Of New Jersey Removal of plastid sequences by transiently expressed site-specific recombinases
WO2005003362A2 (en) 2003-03-10 2005-01-13 Athenix Corporation Methods to confer herbicide resistance
US20040210958A1 (en) * 2003-03-19 2004-10-21 Monsanto Technology Llc A Novel Culture Method for Corn Transformation
US7682829B2 (en) 2003-05-30 2010-03-23 Monsanto Technology Llc Methods for corn transformation
JP3787562B2 (ja) 2003-06-03 2006-06-21 食品工業発展研究所 豆から胚軸を分離するための方法及び装置
DE602004030790D1 (de) 2003-06-16 2011-02-10 Monsanto Technology Llc Verfahren und gerät zur herstellung von genetisch transformierbarem pflanzengewebe
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
US7560611B2 (en) * 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
US8609934B2 (en) 2004-02-13 2013-12-17 Monsanto Technology Llc In vivo assembly of transcription units
US7229034B2 (en) 2004-03-02 2007-06-12 Monsanto Technology Llc Seed crusher
ES2406809T5 (es) 2004-04-30 2017-09-20 Dow Agrosciences, Llc Nuevos genes de resistencia a los herbicidas
MXPA06014380A (es) 2004-06-10 2007-03-08 Univ Toledo Nueva explantacion de semilla dividida de maiz y metodos para la generacion de maiz in vitro.
US7591101B2 (en) 2004-08-26 2009-09-22 Monsanto Technology Llc Automated seed sampler and methods of sampling, testing and bulking seeds
CN1597969A (zh) 2004-09-20 2005-03-23 扬州大学 一种双t－dna载体及其在无选择标记转基因水稻培育中的应用
US20060200878A1 (en) 2004-12-21 2006-09-07 Linda Lutfiyya Recombinant DNA constructs and methods for controlling gene expression
US8993846B2 (en) 2005-09-06 2015-03-31 Monsanto Technology Llc Vectors and methods for improved plant transformation efficiency
AR058989A1 (es) 2006-01-11 2008-03-05 Molecular Plant Breeding Nominees Ltd Metodos para producir celulas y plantas gramineas transgenicas
US7998669B2 (en) 2006-03-02 2011-08-16 Monsanto Technology Llc Automated contamination-free seed sampler and methods of sampling, testing and bulking seeds
AU2007253903B2 (en) 2006-05-16 2012-07-19 Monsanto Technology Llc Use of non-agrobacterium bacterial species for plant transformation
US7524522B2 (en) 2006-08-18 2009-04-28 Mor Technology, Llc Kernel fractionation system
BRPI0808715A8 (pt) * 2007-03-09 2017-04-18 Monsanto Technology Llc Preparação e uso de explantes de embrião de planta para transformação
BRPI0912495A2 (pt) 2008-08-15 2012-11-27 Crown Iron Works Co método para fracionar grãos de milho e método para fracionar grãos de milho em três fluxos de fração de alta pureza

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