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DK2931891T3 - Rna-styret modificering af menneskelige genomer - Google Patents

Rna-styret modificering af menneskelige genomer Download PDF

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Publication number
DK2931891T3
DK2931891T3 DK13863815.0T DK13863815T DK2931891T3 DK 2931891 T3 DK2931891 T3 DK 2931891T3 DK 13863815 T DK13863815 T DK 13863815T DK 2931891 T3 DK2931891 T3 DK 2931891T3
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DK
Denmark
Prior art keywords
rna
human genomes
guided
modification
guided modification
Prior art date
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DK13863815.0T
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English (en)
Inventor
George M Church
Prashant Mali
Luhan Yang
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Harvard College
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Publication of DK2931891T3 publication Critical patent/DK2931891T3/da

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N9/14—Hydrolases (3)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/55—Vectors comprising as targeting moiety peptide derived from defined protein from bacteria
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)

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  • Crystallography & Structural Chemistry (AREA)
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  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK13863815.0T 2012-12-17 2013-12-16 Rna-styret modificering af menneskelige genomer DK2931891T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261738355P 2012-12-17 2012-12-17
US201361779169P 2013-03-13 2013-03-13
PCT/US2013/075317 WO2014099744A1 (en) 2012-12-17 2013-12-16 Rna-guided human genome engineering

Publications (1)

Publication Number Publication Date
DK2931891T3 true DK2931891T3 (da) 2019-08-19

Family

ID=50979072

Family Applications (1)

Application Number Title Priority Date Filing Date
DK13863815.0T DK2931891T3 (da) 2012-12-17 2013-12-16 Rna-styret modificering af menneskelige genomer

Country Status (17)

Country Link
US (19) US9970024B2 (da)
EP (3) EP4282970A3 (da)
JP (4) JP6700788B2 (da)
KR (2) KR20150095861A (da)
CN (1) CN105121641A (da)
AU (5) AU2013363194B2 (da)
CA (2) CA2895155C (da)
DK (1) DK2931891T3 (da)
ES (1) ES2741951T3 (da)
HK (1) HK1212376A1 (da)
IL (2) IL308158A (da)
MX (3) MX2015007743A (da)
MY (1) MY170059A (da)
RU (2) RU2766685C2 (da)
SG (3) SG10201704932UA (da)
WO (2) WO2014099750A2 (da)
ZA (1) ZA201504739B (da)

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