[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

MX2020004325A - Reemplazo dirigido de receptores de células t endógenos. - Google Patents

Reemplazo dirigido de receptores de células t endógenos.

Info

Publication number
MX2020004325A
MX2020004325A MX2020004325A MX2020004325A MX2020004325A MX 2020004325 A MX2020004325 A MX 2020004325A MX 2020004325 A MX2020004325 A MX 2020004325A MX 2020004325 A MX2020004325 A MX 2020004325A MX 2020004325 A MX2020004325 A MX 2020004325A
Authority
MX
Mexico
Prior art keywords
endogenous
cell receptors
targeted replacement
cell
tcr
Prior art date
Application number
MX2020004325A
Other languages
English (en)
Inventor
Theodore Lee Roth
Alexander Marson
Eric Shifrut
Cristina Puig Saus
Antoni Ribas
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Publication of MX2020004325A publication Critical patent/MX2020004325A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/0005—Vertebrate antigens
    • A61K39/0011—Cancer antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/463—Cellular immunotherapy characterised by recombinant expression
    • A61K39/4632—T-cell receptors [TCR]; antibody T-cell receptor constructs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464484—Cancer testis antigens, e.g. SSX, BAGE, GAGE or SAGE
    • A61K39/464488—NY-ESO
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C12N5/0637—Immunosuppressive T lymphocytes, e.g. regulatory T cells or Treg
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/515—Animal cells
    • A61K2039/5156—Animal cells expressing foreign proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/515—Animal cells
    • A61K2039/5158—Antigen-pulsed cells, e.g. T-cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Oncology (AREA)
  • Virology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

En la presente se proporcionan métodos y composiciones para editar el genoma de una célula T humana. En algunas modalidades, se insertan una cadena beta del receptor de células T (TCR-ß) heterólogo y una cadena alfa del receptor de células T (TCR-a) en el exón 1 de un gen constante de la subunidad de TCR en el genoma de la célula T.
MX2020004325A 2017-10-27 2018-10-29 Reemplazo dirigido de receptores de células t endógenos. MX2020004325A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762578153P 2017-10-27 2017-10-27
PCT/US2018/058026 WO2019084552A1 (en) 2017-10-27 2018-10-29 TARGETED REPLACEMENT OF ENDOGENIC T CELL RECEPTORS

Publications (1)

Publication Number Publication Date
MX2020004325A true MX2020004325A (es) 2020-11-09

Family

ID=66247037

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020004325A MX2020004325A (es) 2017-10-27 2018-10-29 Reemplazo dirigido de receptores de células t endógenos.

Country Status (13)

Country Link
US (5) US11033584B2 (es)
EP (1) EP3700924A4 (es)
JP (2) JP7101419B2 (es)
KR (2) KR102503130B1 (es)
CN (2) CN118581047A (es)
AU (2) AU2018355587B2 (es)
BR (1) BR112020008201A2 (es)
CA (1) CA3080415A1 (es)
EA (1) EA202091056A1 (es)
IL (2) IL274179B2 (es)
MX (1) MX2020004325A (es)
SG (1) SG11202003798TA (es)
WO (1) WO2019084552A1 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018283405A1 (en) 2017-06-15 2020-01-16 The Regents Of The University Of California Targeted non-viral DNA insertions
MX2020004325A (es) 2017-10-27 2020-11-09 Univ California Reemplazo dirigido de receptores de células t endógenos.
CN111918659B (zh) 2017-10-30 2024-01-30 派克特制药公司 原代细胞基因编辑
JP7540948B2 (ja) 2018-04-02 2024-08-27 パクト ファーマ インコーポレイテッド ペプチド－ｍｈｃ ｃｏｍｐａｃｔ
US20210207174A1 (en) * 2018-05-25 2021-07-08 The Regents Of The University Of California Genetic engineering of endogenous proteins
EP3924373A4 (en) 2019-02-12 2022-11-30 PACT Pharma, Inc. COMPOSITIONS AND METHODS FOR IDENTIFYING ANTIGEN-SPECIFIC T LYMPHOCYTES
EP4010463A4 (en) * 2019-08-05 2024-05-08 Cartherics Pty. Ltd. IMMUNE CELLS EXPRESSING MODIFIED CELLULAR RECEPTORS AND METHODS OF MANUFACTURING THEREOF
EP3808765A1 (en) * 2019-10-14 2021-04-21 ETH Zurich Cell line for tcr discovery and engineering and methods of use thereof
EP4060038A4 (en) * 2019-11-14 2024-07-10 Univ Hiroshima METHOD FOR INTRODUCING AN ANTIGEN-SPECIFIC RECEPTOR GENE INTO A T CELL GENOME USING CYCLIC DNA
JP2023519819A (ja) * 2020-03-13 2023-05-15 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 標的核酸を改変するための組成物および方法
US20240060089A1 (en) * 2020-08-27 2024-02-22 Tmunity Therapeutics Inc. Vector-free process for manufacture of engineered immune cells
CN116322794A (zh) * 2020-10-02 2023-06-23 加利福尼亚大学董事会 用于改善癌症t细胞免疫疗法的dna构建体
KR20240099259A (ko) 2021-10-14 2024-06-28 아스널 바이오사이언시스, 인크. 공동 발현된 shrna 및 논리 게이트 시스템을 갖는 면역 세포
WO2023154968A2 (en) * 2022-02-14 2023-08-17 The Regents Of The University Of California Dna constructs for improved t cell immunotherapy
TW202417626A (zh) 2022-09-13 2024-05-01 美商亞森諾生物科學公司 具有共表現的ｔｇｆｂｒ ｓｈｒｎａ之免疫細胞
WO2024059824A2 (en) 2022-09-16 2024-03-21 Arsenal Biosciences, Inc. Immune cells with combination gene perturbations
WO2024130179A1 (en) 2022-12-16 2024-06-20 Repertoire Immune Medicines, Inc. T cell receptors binding hpv-16 epitopes
WO2024163935A2 (en) * 2023-02-03 2024-08-08 Affini-T Therapeutics, Inc. Host cells bearing kras binding protein and knockout of endogenous tcr and methods of use thereof
WO2024182219A1 (en) 2023-02-27 2024-09-06 Adaptive Biotechnologies Corp. Therapeutic t cell receptors targeting kras g12d
WO2024186656A1 (en) 2023-03-03 2024-09-12 Arsenal Biosciences, Inc. Systems targeting psma and ca9
WO2024192100A1 (en) 2023-03-13 2024-09-19 Arsenal Biosciences, Inc. Synthetic pathway activators

Family Cites Families (107)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6773669B1 (en) 1995-03-10 2004-08-10 Maxcyte, Inc. Flow electroporation chamber and method
US6090617A (en) 1996-12-05 2000-07-18 Entremed, Inc. Flow electroporation chamber with electrodes having a crystalline metal nitride coating
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
AU2001255748B2 (en) 2000-04-28 2006-08-10 Sangamo Therapeutics, Inc. Methods for binding an exogenous molecule to cellular chromatin
US7029916B2 (en) 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
ES2230502T3 (es) 2001-04-23 2005-05-01 Amaxa Gmbh Disolucion tampon para la electroporacion y procedimiento que incluye el uso de la misma.
WO2003018751A2 (en) 2001-08-22 2003-03-06 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
US20050136040A1 (en) 2001-10-11 2005-06-23 Imperial College Innovations Limited Control of gene expression using a complex of an oligonucleotide and a regulatory peptide
GB0124391D0 (en) 2001-10-11 2001-11-28 Gene Expression Technologies L Control of gene expression
ATE531796T1 (de) 2002-03-21 2011-11-15 Sangamo Biosciences Inc Verfahren und zusammensetzungen zur verwendung von zinkfinger-endonukleasen zur verbesserung der homologen rekombination
ITMI20030821A1 (it) 2003-04-18 2004-10-19 Internat Ct For Genetic En Gineering And Polipeptidi chimerici e loro uso.
US20060182736A1 (en) 2003-06-10 2006-08-17 Kim Jin-Soo Transducible dna-binding proteins
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
DK1577378T3 (da) 2004-03-15 2009-12-07 Lonza Cologne Ag Beholder og indretning til generering af elektriske felter i særskilte reaktionsrum
EP1766057B1 (en) 2004-05-12 2014-12-17 Maxcyte, Inc. Methods and devices related to a regulated flow electroporation chamber
CN103060191B (zh) 2004-06-12 2015-03-11 英杰生命新加坡私人有限公司 一种电穿孔系统
US20070254291A1 (en) 2004-06-14 2007-11-01 Xiaoxia Cui Gene Targeting in Eukaryotic Cells by Group II Intron Ribonucleoprotein Particles
ES2465467T3 (es) 2004-06-14 2014-06-05 Lonza Cologne Ag Procedimiento y disposición de circuito para el tratamiento de material biológico
EP1817406A2 (en) 2004-11-30 2007-08-15 Maxcyte, Inc. Computerized electroporation
US10022457B2 (en) 2005-08-05 2018-07-17 Gholam A. Peyman Methods to regulate polarization and enhance function of cells
DK2325332T3 (da) 2005-08-26 2013-01-28 Dupont Nutrition Biosci Aps Anvendelse af CRISPR-associerede gener (CAS)
EP2049663B1 (en) 2006-08-11 2015-02-25 Dow AgroSciences LLC Zinc finger nuclease-mediated homologous recombination
US8450112B2 (en) 2008-04-09 2013-05-28 Maxcyte, Inc. Engineering and delivery of therapeutic compositions of freshly isolated cells
CN102124102B (zh) 2008-07-18 2013-12-18 麦克赛特股份有限公司 优化电穿孔的方法
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
EP2424877A4 (en) 2009-04-28 2013-01-02 Harvard College SUPERCHARGED PROTEINS FOR CELL PENETRATION
US9181527B2 (en) 2009-10-29 2015-11-10 The Trustees Of Dartmouth College T cell receptor-deficient T cell compositions
US8956828B2 (en) * 2009-11-10 2015-02-17 Sangamo Biosciences, Inc. Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
WO2011072246A2 (en) 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification
WO2011139336A1 (en) 2010-04-26 2011-11-10 Sangamo Biosciences, Inc. Genome editing of a rosa locus using nucleases
US8876458B2 (en) 2011-01-25 2014-11-04 United Technologies Corporation Blade outer air seal assembly and support
US10391126B2 (en) 2011-11-18 2019-08-27 Board Of Regents, The University Of Texas System CAR+ T cells genetically modified to eliminate expression of T-cell receptor and/or HLA
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
US20130236504A1 (en) 2012-03-06 2013-09-12 Medical University Of South Carolina Delivery System for Enhancing Drug Efficacy
PT2800811T (pt) 2012-05-25 2017-08-17 Univ California Métodos e composições para modificação de adn alvo dirigida por arn e para modulação dirigida por arn de transcrição
US20150284727A1 (en) 2012-10-23 2015-10-08 Toolgen Incorporated Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
ES2757325T3 (es) 2012-12-06 2020-04-28 Sigma Aldrich Co Llc Modificación y regulación del genoma en base a CRISPR
EP2931899A1 (en) 2012-12-12 2015-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
EP4286403A3 (en) 2012-12-12 2024-02-14 The Broad Institute Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
EP4282970A3 (en) 2012-12-17 2024-01-17 President and Fellows of Harvard College Rna-guided human genome engineering
IL289396B2 (en) 2013-03-15 2023-12-01 The General Hospital Coporation Using tru-grnas to increase the specificity of RNA-guided genome editing
CA2906218A1 (en) * 2013-03-15 2014-09-18 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
AU2014235968B2 (en) * 2013-03-21 2018-05-24 Ospedale San Raffaele Srl Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
CA2908403A1 (en) 2013-04-02 2014-10-09 Bayer Cropscience Nv Targeted genome engineering in eukaryotes
EP3309248B1 (en) * 2013-05-29 2021-06-09 Cellectis Methods for engineering t cells for immunotherapy by using rna-guided cas nuclease system
ES2883131T3 (es) 2013-05-29 2021-12-07 Cellectis Métodos para la modificación de células T para inmunoterapia utilizando el sistema de nucleasa CAS guiado por ARN
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US10822606B2 (en) 2013-09-27 2020-11-03 The Regents Of The University Of California Optimized small guide RNAs and methods of use
US9957526B2 (en) 2013-10-17 2018-05-01 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering
US9951353B2 (en) 2013-11-15 2018-04-24 The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Engineering neural stem cells using homologous recombination
WO2015089486A2 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems
CN111206032B (zh) 2013-12-12 2024-07-19 布罗德研究所有限公司 用于基因组编辑的crispr-cas系统和组合物的递送、用途和治疗应用
CA2932475A1 (en) 2013-12-12 2015-06-18 The Broad Institute, Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components
EP3080256B1 (en) 2013-12-13 2018-06-13 Cellectis Cas9 nuclease platform for microalgae genome engineering
RU2020104969A (ru) 2014-01-31 2020-03-13 Фэктор Байосайенс Инк. Способы и продукты для получения и доставки нуклеиновых кислот
WO2015115903A1 (en) 2014-02-03 2015-08-06 Academisch Ziekenhuis Leiden H.O.D.N. Lumc Site-specific dna break-induced genome editing using engineered nucleases
EP3800248A3 (en) 2014-04-18 2021-08-04 Editas Medicine, Inc. Crispr-cas-related methods, compositions and components for cancer immunotherapy
US10570418B2 (en) 2014-09-02 2020-02-25 The Regents Of The University Of California Methods and compositions for RNA-directed target DNA modification
SG11201701040XA (en) * 2014-09-19 2017-03-30 Regeneron Pharma Chimeric antigen receptors
WO2016049251A1 (en) 2014-09-24 2016-03-31 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for modeling mutations in leukocytes
CN107002078A (zh) 2014-10-09 2017-08-01 生命技术公司 Crispr寡核苷酸和基因剪辑
WO2016069282A1 (en) 2014-10-31 2016-05-06 The Trustees Of The University Of Pennsylvania Altering gene expression in modified t cells and uses thereof
WO2016097751A1 (en) 2014-12-18 2016-06-23 The University Of Bath Method of cas9 mediated genome engineering
EP3247328A1 (en) 2015-01-21 2017-11-29 PhaseRx, Inc. Methods, compositions, and systems for delivering therapeutic and diagnostic agents into cells
SG11201706059SA (en) 2015-01-30 2017-08-30 Univ California Protein delivery in primary hematopoietic cells
WO2016135559A2 (en) 2015-02-23 2016-09-01 Crispr Therapeutics Ag Materials and methods for treatment of human genetic diseases including hemoglobinopathies
US20180298340A1 (en) 2015-04-24 2018-10-18 The Regents Of The University Of California Systems for detecting, monitoring or treating diseases or conditions using engineered cells and methods for making and using them
MX2017015239A (es) 2015-05-29 2018-02-19 Juno Therapeutics Inc Composicion y metodos para regular interacciones inhibitorias en celulas geneticamente modificadas.
US11643668B2 (en) 2015-06-17 2023-05-09 The Uab Research Foundation CRISPR/Cas9 complex for genomic editing
US11555207B2 (en) 2015-06-17 2023-01-17 The Uab Research Foundation CRISPR/Cas9 complex for introducing a functional polypeptide into cells of blood cell lineage
US20170000743A1 (en) 2015-07-02 2017-01-05 Vindico NanoBio Technology Inc. Compositions and Methods for Delivery of Gene Editing Tools Using Polymeric Vesicles
WO2017011519A1 (en) 2015-07-13 2017-01-19 Sangamo Biosciences, Inc. Delivery methods and compositions for nuclease-mediated genome engineering
US9512446B1 (en) 2015-08-28 2016-12-06 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
WO2017035659A1 (en) 2015-09-01 2017-03-09 The Hospital For Sick Children Method and compositions for removing duplicated copy number variations (cnvs) for genetic disorders and related uses
CA2997749A1 (en) 2015-09-09 2017-03-16 Immune Design Corp. Ny-eso-1 specific tcrs and methods of use thereof
WO2017053729A1 (en) 2015-09-25 2017-03-30 The Board Of Trustees Of The Leland Stanford Junior University Nuclease-mediated genome editing of primary cells and enrichment thereof
WO2017058751A1 (en) 2015-09-28 2017-04-06 North Carolina State University Methods and compositions for sequence specific antimicrobials
ES2920525T3 (es) * 2015-10-05 2022-08-05 Prec Biosciences Inc Células genéticamente modificadas que comprenden un gen de región constante alfa del receptor de linfocitos T humano modificado
WO2017062439A1 (en) * 2015-10-05 2017-04-13 Precision Biosciences, Inc. Engineered meganucleases with recognition sequences found in the human t cell receptor alpha constant region gene
EP3365269A4 (en) 2015-10-19 2019-06-19 The Methodist Hospital DISTRIBUTION, BY MEMBRANE DEFORMATION, FROM CRISPR-CAS9 TO DIFFICULT CELLS TO BE TRANSFERRED
CN108368522A (zh) 2015-10-20 2018-08-03 10X 基因组学有限公司 用于高通量单细胞遗传操作的方法和系统
WO2017070429A1 (en) 2015-10-22 2017-04-27 Regents Of The University Of Minnesota Methods involving editing polynucleotides that encode t cell receptor
CA3003150A1 (en) 2015-11-04 2017-05-11 Fate Therapeutics, Inc. Genomic engineering of pluripotent cells
EP3380613B1 (en) 2015-11-23 2022-10-26 The Regents of the University of California Tracking and manipulating cellular rna via nuclear delivery of crispr/cas9
WO2017106528A2 (en) 2015-12-18 2017-06-22 Sangamo Biosciences, Inc. Targeted disruption of the t cell receptor
JP7449646B2 (ja) 2015-12-30 2024-03-14 アヴェクタス リミテッド 細胞および組織への遺伝子編集タンパク質および組成物の、ベクターなしでの送達
AU2017230011A1 (en) 2016-03-11 2018-09-27 2Seventy Bio, Inc. Genome edited immune effector cells
JP2019510503A (ja) 2016-04-07 2019-04-18 ブルーバード バイオ， インコーポレイテッド キメラ抗原受容体ｔ細胞組成物
US10188749B2 (en) 2016-04-14 2019-01-29 Fred Hutchinson Cancer Research Center Compositions and methods to program therapeutic cells using targeted nucleic acid nanocarriers
CA3020857A1 (en) 2016-04-14 2017-10-19 Fred Hutchinson Cancer Research Center Compositions and methods to program therapeutic cells using targeted nucleic acid nanocarriers
BR112018071199A2 (pt) * 2016-04-15 2019-02-12 Memorial Sloan Kettering Cancer Center célula t, população isolada de células t, composição farmacêutica, método de tratamento, método para gerar uma célula t, vetor, célula tronco
WO2017189336A1 (en) 2016-04-25 2017-11-02 The Regents Of The University Of California Methods and compositions for genomic editing
DK3448990T5 (da) 2016-04-29 2021-09-27 Basf Plant Science Co Gmbh Fremgangsmåder til modifikation af målnukleinsyrer ved anvendelse af et fusionsmolekyle bestående af guide- og donor-dna, rna-fusionsmolekyle og vektorsystemer, der koder for rna-fusionsmolekylet
WO2017210334A1 (en) 2016-05-31 2017-12-07 Massachusetts Institute Of Technology Hydrodynamically controlled electric fields for high throughput transformation & high throughput parallel transformation platform
DK3472338T3 (da) 2016-06-20 2024-07-15 Octapharma Ag Midler og fremgangsmåder til at modifikation af flere alleler
WO2018035387A1 (en) 2016-08-17 2018-02-22 The Broad Institute, Inc. Novel crispr enzymes and systems
WO2018039084A1 (en) 2016-08-20 2018-03-01 The Regents Of The University Of California High-throughput system and method for the temporary permeablization of cells
EP3526235A4 (en) 2016-10-12 2021-03-10 Feldan Bio Inc. RATIONALLY DESIGNED SYNTHETIC PEPTIDE SHUTTLE ACTIVE INGREDIENTS FOR ADMINISTRATION OF POLYPEPTIDE FREIGHT FROM AN EXTRACELLULAR ROOM TO THE CYTOSOL AND / OR THE CORE OF AN EUCARYOTIC TARGET CELL, USES TOGETHER THEREFORE, AND KITS IN THE PROCEDURE
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
WO2018073391A1 (en) 2016-10-19 2018-04-26 Cellectis Targeted gene insertion for improved immune cells therapy
WO2018094291A1 (en) 2016-11-18 2018-05-24 Christopher Bradley Massively multiplexed homologous template repair for whole-genome replacement
CN110582301A (zh) 2016-12-14 2019-12-17 利甘达尔股份有限公司 用于核酸和蛋白质有效负载递送的方法和组合物
EP3562594A4 (en) 2016-12-30 2020-09-09 The Regents of University of California PROCESSES FOR SELECTING AND GENERATING T-LYMPHOCYTES MODIFIED BY THE GENOME
AU2018283405A1 (en) 2017-06-15 2020-01-16 The Regents Of The University Of California Targeted non-viral DNA insertions
MX2020004325A (es) 2017-10-27 2020-11-09 Univ California Reemplazo dirigido de receptores de células t endógenos.
CN111918659B (zh) * 2017-10-30 2024-01-30 派克特制药公司 原代细胞基因编辑

Also Published As

Publication number Publication date
EP3700924A4 (en) 2021-11-03
US11033584B2 (en) 2021-06-15
AU2023202582A1 (en) 2023-05-18
AU2018355587A1 (en) 2020-06-18
JP7101419B2 (ja) 2022-07-15
SG11202003798TA (en) 2020-05-28
IL274179B1 (en) 2023-10-01
KR20230034416A (ko) 2023-03-09
US11083753B1 (en) 2021-08-10
US20210353678A1 (en) 2021-11-18
EA202091056A1 (ru) 2020-09-17
US20220241336A1 (en) 2022-08-04
US20230310502A1 (en) 2023-10-05
JP2021500889A (ja) 2021-01-14
BR112020008201A2 (pt) 2020-10-06
WO2019084552A1 (en) 2019-05-02
CA3080415A1 (en) 2019-05-02
KR20200088348A (ko) 2020-07-22
IL274179B2 (en) 2024-02-01
IL305485A (en) 2023-10-01
US11331346B2 (en) 2022-05-17
CN111655719A (zh) 2020-09-11
IL274179A (en) 2020-06-30
CN118581047A (zh) 2024-09-03
JP2022137112A (ja) 2022-09-21
US11590171B2 (en) 2023-02-28
AU2018355587B2 (en) 2023-02-02
US20210228631A1 (en) 2021-07-29
EP3700924A1 (en) 2020-09-02
US20200000851A1 (en) 2020-01-02
KR102503130B1 (ko) 2023-02-24

Similar Documents

Publication Publication Date Title
MX2020004325A (es) Reemplazo dirigido de receptores de células t endógenos.
CO2021006363A2 (es) Composiciones y métodos para la expresión de transgenes a partir de un locus de albúmina
CO2017001573A2 (es) Receptor quimérico de antígeno anti-cd123
BR112022021676A2 (pt) Métodos de distribuição de célula in vitro
DK3359184T3 (da) Genetisk modificerede celler, der omfatter et modificeret gen fra konstant alfaregion i human t-cellereceptor
CL2016003247A1 (es) Composiciones y métodos para la expresión de arns guía usando el promotor h1
PH12016502162A1 (en) Multiplex gene editing in swine
CO2018012996A2 (es) Agonista del receptor de glucocorticoides e inmunoconjugados del mismo
CO2017000507A2 (es) Receptor quimérico de antígeno bcma
SG11202106787SA (en) Receptors providing targeted costimulation for adoptive cell therapy
GB2531178A (en) Improvements to portable power supply
CO2017000510A2 (es) Constructos de car
MX2018005129A (es) Agonistas del receptor de glucagon.
SG10201900455YA (en) Bcma chimeric antigen receptors
PE20150722A1 (es) Moduladores de la expresion de receptores androgenicos que comprenden un oligonucleotido modificado
CL2020000375A1 (es) Activadores de piruvato quinasas para usar en el tratamiento de trastornos en la sangre.
AR069474A1 (es) Material anticorrosivo
UY37351A (es) Complemento para el pienso
IL277883A (en) Optimized engineered nucleases with specificity for the human T cell receptor alpha constant region gene
MY183188A (en) Non-human animals having a humanized b-cell activating factor gene
MX2020005849A (es) Células madre y progenitoras hematopoyéticas humanas cd34+ modificadas con crispr-cas9 y sus usos.
IL282914A (en) T cell preparations with improved phenotypic characteristics
PH12017502099A1 (en) Methods for diagnosing and assessing treatment for cushing's syndrome
IL290109A (en) Cells for enhanced immune healing and their uses
MX2020011470A (es) Metodos de terapia genica.