CN102998473A - Full-automatic chemiluminescence immune analyzer - Google Patents
Full-automatic chemiluminescence immune analyzer Download PDFInfo
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- CN102998473A CN102998473A CN201210556838XA CN201210556838A CN102998473A CN 102998473 A CN102998473 A CN 102998473A CN 201210556838X A CN201210556838X A CN 201210556838XA CN 201210556838 A CN201210556838 A CN 201210556838A CN 102998473 A CN102998473 A CN 102998473A
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Abstract
The invention relates to a full-automatic chemiluminescence immune analyzer. The full-automatic chemiluminescence immune analyzer comprises a sample injection part, a reaction control part and an optical detection part, wherein the sample injection part comprises reaction cup storage cabins, a sample conveying device, a reagent disc, a sample diluting device, mechanical grippers, a sample needle, a reagent needle, a magnetic bead stirring mechanism, a diluent needle and a diluent cabin, wherein the reaction control part consists of a reaction cup incubating disc and a magnetic bead cleaning disc; the optical detection part consists of an optical detection disc, a substrate needle and a substrate cabin; and all parts are mounted on a stander, and the sample injection part and the optical detection part are respectively located at two sides of the reaction control part. According to the full-automatic chemiluminescence immune analyzer, an independent optical detection system, an independent magnetic bead cleaning system and an independent sample diluting system are adopted, the structure is optimized, and the control is simpler; and the full-automatic chemiluminescence immune analyzer has the characteristics of strong function, high sensitivity and low cost, and the development of the chemiluminescence immune analyzer field can be greatly promoted.
Description
Technical field
The present invention relates to Full-automatic chemiluminescence immunity class detection system or instrument, particularly a kind of Full-automatic chemiluminescence immunoassay analysis meter.Be widely used in clinical labororatory and R﹠D institution to monitoring analysis of hormone, virus marker thing, tumor markers and medicine etc.
Background technology
Chemiluminescence immunoassay technology (CLIA) is worldwide to develop very fast on-radiation immuno analytical method over past ten years, is a kind of supersensitive microdetermination technology that grows up after Enzyme-multiplied immune technique (EIA), radioimmunoassay technique (RIA), immunofluorence technic (FIA).This technology utilizes chemistry or bio-luminescence system as the indication mechanism of antigen-antibody reaction, so as to the method for quantitative detectable antigens or antibody.Luminescent substance can be directly as the label of antigen or antibody, and the form that also can dissociate is used for catalyzer and the antibody of assistant mark or the luminescence-producing reaction of antigen.The free energy that chemiluminescence reaction discharges excites intermediate, makes it get back to ground state from excited state, the photon of energy level such as discharges, through the mensuration of chemo-immunity analyser to photon, and accurate quantification measured object content.The automatic chemiluminescence immunoassay technology integrates classical methodology and advanced technology, has the outstanding advantages such as highly sensitive, that preci-sion and accuracy is high, reagent stability good, automaticity is high.
Do not go out its right person on the existing various immuno analytical methods at present, developed countries is making much progress aspect the research and development of CLIA now, the series of products such as existing full automatic chemiluminescence and electrochemiluminescence immunoassay.At present, the full-automatic instrument of chemiluminescence immune assay is offshore company monopolization substantially, does not also have ripe chemical illumination immunity analysis instrument to put goods on the market in China, all is the imported product of high price on the market, and domestic only have corresponding accurate robot.Therefore, develop a kind of Full-automatic chemiluminescence immunoassay analysis meter and become a domestic megatrend.
Summary of the invention
The object of the invention is to, a kind of more optimized structure is provided, control simplyr, measuring accuracy is higher, the Full-automatic chemiluminescence immunoassay analysis meter that price is cheaper.
The present invention is achieved through the following technical solutions: a kind of Full-automatic chemiluminescence immunoassay analysis meter, comprise sample introduction part, reaction control section and optical detection part, sample introduction partly comprises reaction cup warehouse, sample delivery device, reagent disc, Sample Dilution device, mechanical gripper, specimen needle, reagent needles, magnetic bead rabbling mechanism, dilution pin and dilution storehouse; The reaction control section is comprised of reaction cup incubation dish, magnetic bead cleaning disc; The optical detection part is comprised of optical detection dish, substrate pin and substrate storehouse; Above-mentioned all parts are installed on the frame, and this sample introduction part and optical detection partly lay respectively at the both sides of reaction control section.
Described reaction cup warehouse, reagent disc, reaction cup incubation dish, magnetic bead cleaning disc and the setting of optical detection dish are at grade; Described specimen needle, reagent needles, dilution pin and substrate pin all can be rotated and elevating movement, and the liquid reagents such as sample are quantitatively drawn and discharged, and realize the quantitative transfer of each liquid reagent.
But described optical detection dish comprises the Mi Guangcang of automatic shutter, the detection dish that can rotate in the close smooth storehouse, and can realize device optically focused lens barrel that light converges and as the photon counter of photon detection device, the reaction cup that is placed with test sample is put on the detection dish, the detection dish is rotated motion, in this process, implement the interpolation of substrate, by the mutual bump friction between reaction cup and the friction block, realize the mixing of substrate and magnetic bead, forward the light signal that converges collection behind the detection position by optical tubes to, by photon counter the light signal of collecting is changed into electric signal.
Described mechanical gripper has two: a mechanical gripper that is located between reaction cup warehouse, Sample Dilution device and the reaction cup incubation dish, and another is located at the mechanical gripper between reaction cup incubation dish, magnetic bead cleaning disc and the optical detection dish; Described mechanical gripper and all can horizontal rectilinear motion and vertical motion can carry out grasping movement to reaction cup, the transfer of realization response cup between different device simultaneously.
Described magnetic bead cleaning disc comprises reaction cup rotating disc, lifting punch block, magnetic bead adsorbent equipment and mixing friction wheel, the lifting punch block drives by spindle motor and vertically moves up and down along line slide rail, at the lifting punch block four groups of cleaning needle assemblies (93a, 93b, 93c, 93d) is installed; Described reaction cup rotating disc drives reaction cup and rotates; Each described cleaning needle assembly comprises that one is used for the fluid injection pin of fluid injection and the aspirating needle of imbibition, by the waste liquid in the aspirating needle absorption reaction cup, injects quantitative cleaning fluid by the fluid injection pin in reaction cup more first; Described mixing friction wheel is driven by stepper motor, is fixed on the flexible electric machine support, and the mixing friction wheel has three groups, lays respectively at the position behind the cleaning needle assembly (93a, 93b, 93c), is used for the reaction cup that adds cleaning fluid is carried out mixing; On the described magnetic bead adsorbent equipment a plurality of magnet are installed, the magnetic bead in the reaction cup are carried out magnetic attached, the magnetic bead in the reaction cup is separated with solution; Stepper motor drives the mixing friction wheel vibration is rotated and produced to the friction force drive reaction cup of reaction cup, guarantees that the interior solution of reaction cup mixes; Described mixing friction pulley material is elastic caoutchouc.
Sample Dilution device in the described sample introduction part, dilution storehouse and dilution pin form a Sample Dilution system, in the time of need to carrying out Sample Dilution, mechanical gripper 4a picks up on the dilution frame that a new reaction cup is put into the Sample Dilution device from the reaction cup warehouse, the dilution frame can be done straight reciprocating motion at line slide rail with Timing Belt, specimen needle is at first drawn quantitative serum and is injected the reaction cup of diluting on the frame, then the dilution pin is drawn quantitative dilution injection reaction cup from the dilution storehouse, last mixing mechanism carries out the mixing action to reaction cup, after mixing is finished, specimen needle is drawn quantitatively, and the reaction cup of dilute sample injection reaction cup incubation dish detects, dilute subsequently frame and move to the top of pipe abandon upper port, automatically the abandoning of realization response cup.
Described reaction cup warehouse is divided into the supplying module of two independent controls, with the sustainable replacing of realization response cup consumable.
Described magnetic bead rabbling mechanism is comprised of direct current generator and puddler, and direct current generator links to each other with puddler, drives puddler and rotates, and is used for the magnetic bead liquid in the reagent disc is stirred, and makes it even mixing.
Advantage of the present invention and good effect are:
1, structure optimization, control is simple.The present invention adopts independently Systems for optical inspection, magnetic bead purging system and Sample Dilution system, has optimized structure, makes control simpler.2, precision is high.The problems such as the present invention adopts independently Systems for optical inspection, solves in the existing full-automatic immunoluminescence analyser, and detection architecture in place causes between pipe and crosstalks, background value is higher, the sensitivity that the raising system detects.
3, the magnetic bead cleaning is cleaner, and loss is less.The present invention adopts independently magnetic bead cleaning device, makes the debugging maintenance more convenient, has simplified testing process, has improved the instrument work efficiency.
4, have the advantages that function is strong, cost is low, can greatly promote the development in chemical illumination immunity analysis instrument device field.
Description of drawings
Fig. 1 is general structure schematic diagram of the present invention;
Fig. 2 is the structural representation of magnetic bead cleaning disc of the present invention
Fig. 3 is the structural representation of optical detection dish of the present invention
Fig. 4 is the structural representation of dilution system of the present invention
Fig. 5 is workflow diagram of the present invention.
Embodiment
The invention will be further described below in conjunction with accompanying drawing.
A specific embodiment of the complete machine structure of Full-automatic chemiluminescence immunoassay analysis meter of the present invention as shown in Figure 1, comprise: sample introduction part, reaction control section and optical detection part, wherein the sample introduction part is by reaction cup warehouse 3(3a and 3b), sample delivery device 1, reagent disc 12, Sample Dilution device 5, mechanical gripper 4(4a and 4b), specimen needle 6, reagent needles 8, magnetic bead rabbling mechanism 13, dilution pin 2 and dilution storehouse 14 etc. form; The reaction control section is comprised of reaction cup incubation dish 7, magnetic bead cleaning disc 9; The optical detection part is comprised of optical detection dish 10, substrate pin 11 and substrate storehouse 15 etc.
Referring to Fig. 2, independently magnetic bead purging system of the present invention is that magnetic bead cleaning disc 9 comprises reaction cup rotating disc 91, lifting punch block 94, magnetic bead adsorbent equipment 95 and mixing friction wheel 96.Lifting punch block 94 drives by spindle motor 99 and vertically moves up and down along line slide rail 92, and the interval is equipped with four groups of cleaning needle assembly 93(93a, 93b, 93c and 93d successively on half round edge of semicircular lifting punch block 94).Reaction cup rotating disc 91 is rotatably installed in above the base 100 (driving its rotation by drive unit during work), is used for driving reaction cup and rotates.The bottom of each cleaning needle assembly 93 is provided with one and is used for the fluid injection pin 931 of fluid injection and the aspirating needle 932 of an imbibition, by the waste liquid in the aspirating needle absorption reaction cup, injects quantitative cleaning fluid by the fluid injection pin in reaction cup more first.Mixing friction wheel 96 is driven by stepper motor 98, and stepper motor 98 is fixed on the flexible electric machine support 97, and the lower end of electric machine support 97 is connected with base 100.Mixing friction wheel 96 has three groups, lays respectively at the back of three cleaning needle assemblies 93, is used for the reaction cup that adds cleaning fluid is carried out mixing (driving reaction cup rotates).A plurality of magnet 910 are installed on the magnetic bead adsorbent equipment 95, the magnetic bead in the reaction cup are carried out magnetic attached, the magnetic bead in the reaction cup is separated with solution.Cleaning step is as follows: at first the reaction cup of putting into reaction cup rotating disc 91 is carried out magnetic attached, by the waste liquid in the rightmost cleaning needle assembly 93a absorption reaction cup, cleaning fluid reinjects, then by first mixing friction wheel 96 to its mixing that vibrates, it is attached that mixing afterreaction cup rotates again magnetic with rotating disc 91, again by the waste liquid in the second right cleaning needle assembly 93b absorption reaction cup, cleaning fluid reinjects, so repeatedly, by the cleaning fluid that do not reinject behind the waste liquid in the leftmost cleaning needle assembly 93d absorption reaction cup, finish the magnetic bead in the reaction cup is cleaned.
Referring to Fig. 3, optical detection dish 10 among the present invention, substrate pin 11 and substrate storehouse 15 form independently Systems for optical inspection, and wherein optical detection dish 10 comprises rotatable detection dish 102 in the close smooth storehouse 101 that can automatically open/close, the close smooth storehouse, close light lid 103, upper fan door 104, can realize device optically focused lens barrel 110, photon detection device photon counter 111 that light converges.Wherein close smooth storehouse 101, close light lid 103, upper fan door 104 form a close optical cavity body, and detection dish 102 is driven by stepper motor 106, can freely rotate in above-mentioned cavity.Upper fan door 104 is realized the opening and closing movement of close optical cavity body by stepper motor 105 driven rotary.The specific works process is as follows: at first detection dish 102 forwards to and adds the reaction cup position, upper fan door 104 is opened, mechanical gripper 4b puts on the detection dish 102 from magnetic bead cleaning disc 9 crawl reaction cup, upper fan door 104 closures, detection dish 102 rotates 180 ° of interpolations of carrying out substrate (substrate pin 11 takes out the reaction cup 109 that substrate adds on the detection dish 102 from substrate storehouse 15 interior), add complete after, detection dish 102 is interior reciprocating rotation among a small circle, made by elastomeric material by reaction cup 109 and friction block 108() between bump friction realize the mixing of substrate and magnetic bead, close light box 107 prevents that ambient light from entering in the Mi Guangcang, after mixing is finished, forward the detection position to, optical tubes 110 converges the light signal of collection, by photon counter 111 light signal of collecting is changed into electric signal, abandon reaction cup by mechanical gripper 4b crawl after detection is finished.Be characterized in, realize dynamic close light, can satisfy entering and abandoning of outside reaction cup to be detected, the reagent of close luminous environment adds and mixing, has realized dynamic close light, multi-functional detection demand in full-automatic illumination class detection system.
Referring to Fig. 4, Sample Dilution device 5, dilution storehouse 14 and dilution pin 2 in the sample introduction part of the present invention form independently Sample Dilution system, this Sample Dilution system comprises: Sample Dilution device 5, dilution storehouse 14 and dilution pin 2, dilution storehouse 14 and dilution pin 2 are arranged on an end of Sample Dilution device 5.In the time of need to carrying out Sample Dilution, mechanical gripper 4a picks up on the dilution frame 503 that a new reaction cup is put into Sample Dilution device 5 from reaction cup warehouse 3, and dilution frame 503 can be done straight reciprocating motion at line slide rail 502 with Timing Belt 505.At first specimen needle 6 is drawn the reaction cup on the quantitative serum injection dilution frame 503, then dilution pin 2 is drawn quantitative dilution injection reaction cup from dilution storehouse 14, last 504 pairs of reaction cup of mixing mechanism (structure is identical with mixing friction wheel 96) are carried out the mixing action, after mixing is finished, specimen needle 6 is drawn quantitatively, and the reaction cup of dilute sample injection reaction cup incubation dish 7 detects, dilute subsequently frame 503 and move to the top of pipe abandon 501 upper port, reaction cup falls into pipe abandon 501, automatically the abandoning of realization response cup.Described reaction cup is disposal reaction cup.
Analyser also needs a control circuit and control software thereof, is used for control and commander's reaction cup warehouse 3, sample delivery device 1, reagent disc 12, Sample Dilution device 5, mechanical gripper 4, specimen needle 6, reagent needles 8, magnetic bead rabbling mechanism 13, dilution pin 2 and the each several parts such as dilution storehouse 14, reaction cup incubation dish 7, magnetic bead cleaning disc 9, optical detection dish 10, substrate pin 11 and substrate storehouse 15 according to the needs coordinated work of chemiluminescence immune assay.
The remaining part of the present invention of not mentioning in the above description and device are this area routine techniquess.
In the instrument course of work, the action of each parts is undertaken by the instrument workflow, and Fig. 5 is the workflow diagram of full-automatic chemical immunoluminescence analytical instrument of the present invention, and package unit both can have been supported the single stage method test in conjunction with this flow process, also can support the two-step approach test.Whole instrument concrete operation step is as follows:
A. instrument start, system initialization specifically comprises: reaction cup warehouse 3a and 3b, sample delivery device 1, reagent disc 12, Sample Dilution device 5, mechanical gripper 4a and 4b, specimen needle 6, reagent needles 8, magnetic bead rabbling mechanism 13, dilution pin 2, reaction cup incubation dish 7, magnetic bead cleaning disc 9, optical detection dish 10, substrate pin 11 carry out mechanical return; Optical detection dish 10 background value of testing oneself; Background value is up to standard, and system just allowed to begin test after reaction cup incubation dish 7 reached 37 ° of C of constant temperature;
B. system prompt mechanical arm 4a places the new reaction cup in reaction cup incubation dish 7;
C. specimen needle 6 is drawn the reaction cup that sample injects reaction cup incubation dish 7, cleaning probe after application of sample is finished from sample hose;
D. reaction cup incubation dish 7 rotates, and after reaction cup forwards the reagent adding position to, successively draws reagent and magnetic bead injection reaction cup the reagent bottle of reagent needles 8 from reagent disc 12, cleaning probe after application of sample is finished;
E. then reaction cup incubation dish 7 drives reaction cup and rotates, and the potpourri in the reaction cup heats in reaction tray reached it in 1 minute or near 37 ° of C, and the incubation certain hour;
F. after incubation was finished, reaction cup incubation dish 7 drove reaction cup and forwards out the pipe position to, and mechanical arm 4b picks up reaction cup and is put in the magnetic bead cleaning disc 9, the magnetic bead in the reaction cup is carried out three times clean;
G. after cleaning was finished, mechanical arm 4b picked up reaction cup and is put in the optical detection dish 10, and substrate pin 11 injects luminous substrate in reaction cup, and then mixing detects, and after detection is finished, abandoned reaction cup;
H. calculate and export measurement result.
Claims (8)
1. Full-automatic chemiluminescence immunoassay analysis meter, comprise sample introduction part, reaction control section and optical detection part, sample introduction partly comprises reaction cup warehouse, sample delivery device, reagent disc, Sample Dilution device, mechanical gripper, specimen needle, reagent needles, magnetic bead rabbling mechanism, dilution pin and dilution storehouse; The reaction control section is comprised of reaction cup incubation dish, magnetic bead cleaning disc; The optical detection part is comprised of optical detection dish, substrate pin and substrate storehouse; Above-mentioned all parts are installed on the frame, and this sample introduction part and optical detection partly lay respectively at the both sides of reaction control section.
2. Full-automatic chemiluminescence immunoassay analysis meter according to claim 1, it is characterized in that: described reaction cup warehouse, reagent disc, reaction cup incubation dish, magnetic bead cleaning disc and the setting of optical detection dish are at grade; Described specimen needle, reagent needles, dilution pin and substrate pin all can be rotated and elevating movement, and the liquid reagents such as sample are quantitatively drawn and discharged, and realize the quantitative transfer of each liquid reagent.
3. Full-automatic chemiluminescence immunoassay analysis meter according to claim 1, it is characterized in that: but described optical detection dish comprises the Mi Guangcang of automatic shutter, the detection dish that can rotate in the close smooth storehouse, and can realize device optically focused lens barrel that light converges and as the photon counter of photon detection device, the reaction cup that is placed with test sample is put on the detection dish, the detection dish is rotated motion, in this process, implement the interpolation of substrate, by the mutual bump friction between reaction cup and the friction block, realize the mixing of substrate and magnetic bead, forward the light signal that converges collection behind the detection position by optical tubes to, by photon counter the light signal of collecting is changed into electric signal.
4. Full-automatic chemiluminescence immunoassay analysis meter according to claim 1, it is characterized in that: described mechanical gripper has two: a mechanical gripper that is located between reaction cup warehouse, Sample Dilution device and the reaction cup incubation dish, and another is located at the mechanical gripper between reaction cup incubation dish, magnetic bead cleaning disc and the optical detection dish; Described mechanical gripper and all can horizontal rectilinear motion and vertical motion can carry out grasping movement to reaction cup, the transfer of realization response cup between different device simultaneously.
5. Full-automatic chemiluminescence immunoassay analysis meter according to claim 1, it is characterized in that: described magnetic bead cleaning disc comprises reaction cup rotating disc, lifting punch block, magnetic bead adsorbent equipment and mixing friction wheel, the lifting punch block drives by spindle motor and vertically moves up and down along line slide rail, at the lifting punch block four groups of cleaning needle assemblies (93a, 93b, 93c, 93d) is installed; Described reaction cup rotating disc drives reaction cup and rotates; Each described cleaning needle assembly comprises that one is used for the fluid injection pin of fluid injection and the aspirating needle of imbibition, by the waste liquid in the aspirating needle absorption reaction cup, injects quantitative cleaning fluid by the fluid injection pin in reaction cup more first; Described mixing friction wheel is driven by stepper motor, is fixed on the flexible electric machine support, and the mixing friction wheel has three groups, lays respectively at the position behind the cleaning needle assembly (93a, 93b, 93c), is used for the reaction cup that adds cleaning fluid is carried out mixing; On the described magnetic bead adsorbent equipment a plurality of magnet are installed, the magnetic bead in the reaction cup are carried out magnetic attached, the magnetic bead in the reaction cup is separated with solution; Stepper motor drives the mixing friction wheel vibration is rotated and produced to the friction force drive reaction cup of reaction cup, guarantees that the interior solution of reaction cup mixes; Described mixing friction pulley material is elastic caoutchouc.
6. Full-automatic chemiluminescence immunoassay analysis meter according to claim 1, it is characterized in that: the Sample Dilution device in the described sample introduction part, dilution storehouse and dilution pin form a Sample Dilution system, in the time of need to carrying out Sample Dilution, mechanical gripper 4a picks up on the dilution frame that a new reaction cup is put into the Sample Dilution device from the reaction cup warehouse, the dilution frame can be done straight reciprocating motion at line slide rail with Timing Belt, specimen needle is at first drawn quantitative serum and is injected the reaction cup of diluting on the frame, then the dilution pin is drawn quantitative dilution injection reaction cup from the dilution storehouse, last mixing mechanism carries out the mixing action to reaction cup, after mixing is finished, specimen needle is drawn quantitatively, and the reaction cup of dilute sample injection reaction cup incubation dish detects, dilute subsequently frame and move to the top of pipe abandon upper port, automatically the abandoning of realization response cup.
7. Full-automatic chemiluminescence immunoassay analysis meter according to claim 1 is characterized in that: described reaction cup warehouse is divided into the supplying modules of two independent controls, with the sustainable replacing of realization response cup consumable.
8. Full-automatic chemiluminescence immunoassay analysis meter according to claim 1, it is characterized in that: described magnetic bead rabbling mechanism is comprised of direct current generator and puddler, direct current generator links to each other with puddler, the drive puddler rotates, be used for the magnetic bead liquid in the reagent disc is stirred, make it even mixing.
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