CN108646039B - Full-automatic protein chip immunity analyzer - Google Patents
Full-automatic protein chip immunity analyzer Download PDFInfo
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- CN108646039B CN108646039B CN201810716256.0A CN201810716256A CN108646039B CN 108646039 B CN108646039 B CN 108646039B CN 201810716256 A CN201810716256 A CN 201810716256A CN 108646039 B CN108646039 B CN 108646039B
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 27
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 27
- 230000036039 immunity Effects 0.000 title claims abstract description 7
- 230000007246 mechanism Effects 0.000 claims abstract description 123
- 238000006243 chemical reaction Methods 0.000 claims abstract description 115
- 239000003085 diluting agent Substances 0.000 claims abstract description 87
- 239000007788 liquid Substances 0.000 claims abstract description 69
- 238000004140 cleaning Methods 0.000 claims abstract description 48
- 210000002966 serum Anatomy 0.000 claims abstract description 41
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 36
- 238000001035 drying Methods 0.000 claims abstract description 15
- 239000002351 wastewater Substances 0.000 claims description 35
- 238000010438 heat treatment Methods 0.000 claims description 29
- 239000000523 sample Substances 0.000 claims description 25
- 238000002791 soaking Methods 0.000 claims description 23
- 230000000903 blocking effect Effects 0.000 claims description 20
- 238000009413 insulation Methods 0.000 claims description 20
- 239000013307 optical fiber Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 238000000861 blow drying Methods 0.000 claims description 15
- 229910001220 stainless steel Inorganic materials 0.000 claims description 13
- 239000010935 stainless steel Substances 0.000 claims description 13
- 238000002347 injection Methods 0.000 claims description 12
- 239000007924 injection Substances 0.000 claims description 12
- 230000002572 peristaltic effect Effects 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 6
- 238000005086 pumping Methods 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 2
- 238000013016 damping Methods 0.000 claims 1
- 238000005057 refrigeration Methods 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 206010011409 Cross infection Diseases 0.000 abstract description 2
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 abstract 1
- 238000003018 immunoassay Methods 0.000 description 24
- 238000004020 luminiscence type Methods 0.000 description 17
- 238000010586 diagram Methods 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 4
- 238000002372 labelling Methods 0.000 description 3
- 230000035939 shock Effects 0.000 description 3
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000036046 immunoreaction Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- -1 code scanning Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 230000004850 protein–protein interaction Effects 0.000 description 1
- 238000006862 quantum yield reaction Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
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- Engineering & Computer Science (AREA)
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention relates to a full-automatic protein chip immunity analyzer, which is characterized in that a cover plate is arranged at the top of a shell, a serum disc, a reaction disc, a reagent disc and a chip CCD shooting mechanism are sequentially arranged on the cover plate, a chip feeding mechanism, a reaction cup feeding mechanism, a diluent storage and filling mechanism, a sample feeding mechanism and a serum arm are arranged on the outer side of the surrounding serum disc, a cleaning tank is arranged on one side of the serum arm, a chip arm is arranged between the serum disc and the reaction disc, a chip cleaning and drying mechanism and a cup removing mechanism are arranged on one side of the chip arm, a reagent arm is arranged between the reaction disc and the reagent disc, a cleaning tank is arranged on one side of the reagent arm, a chip arm is arranged on one side of the reaction disc, a cup removing mechanism and a chip cleaning and drying mechanism are arranged on one side of the chip arm, a chip arm is arranged on one side of the chip CCD shooting mechanism, a drying mechanism, a luminous liquid reaction mechanism and a garbage box are arranged on the side of the shell. The invention has the advantages that: convenient operation and cross infection prevention. The reaction stability and accuracy are improved, and the efficiency is high.
Description
Technical Field
The invention relates to a full-automatic protein chip immunoassay instrument.
Background
The protein chip is a high-flux protein function analysis technology, and can be used for protein expression profile analysis, research on protein-protein interaction, even DNA-protein and RNA-protein interaction, screening of protein targets with medicine effect and the like.
The protein chip adopts an immunoassay instrument to carry out immunoassay, the immunoassay instrument specifically refers to an instrument for carrying out chemiluminescence labeling immunoassay (also called chemiluminescence immunoassay), and the immunoassay instrument is an immunoassay method for directly labeling antigens or antibodies by using a chemiluminescence agent.
The protein chip immunoassays of the prior art generally comprise two parts, namely an immunoreaction system and a chemiluminescent assay system. The immunoreaction system is used for directly labeling a luminescent substance (an excited state intermediate generated under excitation of a reaction solution) on an antigen (chemiluminescent immunoassay) or an antibody (immunochemical luminescent assay), or acting an enzyme on a luminescent substrate. The chemiluminescent analysis system is used for forming an excited intermediate by catalyzing a chemiluminescent substance through a catalyst and oxidizing the oxidant, and simultaneously emitting photons (hM) when the excited intermediate returns to a stable ground state, and measuring the light quantum yield by using a luminescent signal measuring instrument.
In the prior art, the protein chip immunity analyzer is generally arranged by separating each system and each mechanism, the operation needs multiple manual operations, the efficiency is low, the time is long, and the full-automatic operation cannot be realized.
Disclosure of Invention
The invention provides a full-automatic protein chip immunoassay instrument, which aims to overcome the defects in the prior art and realize the full-automatic implementation of the protein chip immunoassay instrument from the filling of serum to the obtaining of an analysis structure.
The technical solution of the invention is as follows: the full-automatic protein chip immunity analyzer structurally comprises a shell, wherein a cover plate is arranged at the top of the shell, an opening and closing cover is arranged on the cover plate, a display and an operator are arranged on one side of the shell, a serum tray, a reaction tray, a reagent tray and a chip CCD shooting mechanism are sequentially arranged on the cover plate, a chip feeding mechanism, a reaction cup feeding mechanism, a diluent storage and filling mechanism, a sample injection mechanism and a serum arm are arranged on the outer side of the surrounding serum tray, a cleaning tank is arranged on one side of the serum arm, a sample scanning head is arranged on the sample injection mechanism, a chip arm is arranged between the serum tray and the reaction tray, a chip cleaning and drying mechanism and a cup removing mechanism are arranged on one side of the chip arm, a reagent arm is arranged between the reaction tray and the reagent tray, a cleaning tank is arranged on one side of the reagent arm, a chip arm is arranged on one side of the reaction tray, a chip arm is arranged on one side of the chip CCD shooting mechanism, a drying mechanism, a luminescent liquid reaction mechanism and a garbage box are arranged on one side of the shell, and a reaction area temperature control mechanism is arranged on one side of the shell.
Preferably, the chip feeding mechanism comprises an A heat insulation plate, a screw rod motor, a chip frame, an original point sensor, an A optical fiber sensor, an upper chip bouncing cylinder, a lower chip bouncing cylinder and a chip frame left and right movement cylinder, wherein a supporting leg is arranged at the bottom of the A heat insulation plate, a left and right sliding rail is arranged in the A heat insulation plate, a supporting frame is movably connected to the left and right sliding rail, the side face of the supporting frame is connected with the chip frame left and right movement cylinder, two mutually parallel chip frames are arranged on the supporting frame, the chip frames are limited by a locating pin and the supporting frame, a chip baffle is arranged in the chip frames, a notch matched with a notch on the A chip is arranged on the chip baffle, one end of the chip frame is provided with the A optical fiber sensor, the chip bouncing sheet is arranged below the chip frame close to the A optical fiber sensor, the upper chip bouncing sheet and the lower chip bouncing cylinder are connected with the lower chip bouncing cylinder, the upper chip bouncing cylinder and the lower chip bouncing cylinder are arranged in the A heat insulation plate, the other end of the chip frame is provided with the original point sensor, a sensor is arranged beside the original point sensor, a sensor light blocking block is arranged on the inner side of the chip frame below the original point sensor, the chip frame is connected with a screw rod motor, the screw rod is arranged on the side of the chip frame, the chip baffle is parallel to the motor, and the chip sensor is arranged on the side of the chip frame close to the side wall of the A heat insulation plate.
Preferably, the chip cleaning and blow-drying mechanism comprises a cleaning box, a chip placing frame, a chip soaking tank and a jet head, wherein the chip soaking tank is arranged in the middle of the cleaning box, the chip placing frame is arranged above the chip soaking tank, a B chip is placed on the chip placing frame, stainless steel needle tubes are respectively arranged on tank walls on two sides of the chip soaking tank, the stainless steel needle tubes are externally connected with cleaning liquid diaphragm pumps, liquid outlets of the stainless steel needle tubes on two sides are oppositely arranged, the jet head is respectively arranged on two sides of the chip placing frame on the upper side of the stainless steel needle tubes, the jet head is connected with an air pipe joint, the air pipe joint is externally connected with a blow-drying device with an electromagnetic valve, the bottom of the chip soaking tank and the bottom of the cleaning box on the outer side of the chip soaking tank are respectively connected with a waste water joint, the waste water joint on the bottom of the chip soaking tank is connected with a diaphragm valve, the two waste water joints are externally connected with a waste water pumping diaphragm pump, the bottom of the cleaning box is provided with a jet distance adjusting block, and the jet distance adjusting block is connected with the jet heads on two sides.
Preferably, reaction cup feeding mechanism include sharp slip table, reaction cup vibration dish, directly shake the ware, control the drive block, linear guide, upper and lower drive block, reaction cup placer and reaction cup clamping jaw, wherein reaction cup vibration dish and directly shake the ware below straight slip table one end, reaction cup vibration dish track end-to-end connection directly shake the ware, directly shake the ware export and establish the reaction cup placer, sensor mount pad is established in the reaction cup placer outside, establish first photoelectric sensor on the sensor mount pad, reaction cup spacer is established by reaction cup placer, the optical fiber mounting bracket is established in the reaction cup spacer outside, establish B optical fiber sensor on the optical fiber mounting bracket, tank chain mounting plate is established to the linear guide side, sliding connection has control drive block on the linear slip table of tank chain mounting plate inboard, first step motor and second photoelectric sensor are established to the first step motor connection drive left and right sides the drive block, establish the drive bracket on the drive bracket, establish the second step motor on the drive beam, drive down drive motor is equipped with upper and lower drive block, establish linear guide on the upper and lower drive block, the upper and lower drive block is connected with the cylinder top, the cylinder is connected to the cylinder top down.
Preferably, the luminous liquid reaction mechanism comprises a luminous liquid pool, an A luminous liquid needle tube, a B luminous liquid needle tube, a pure water needle tube and a waste water hole, wherein the top of three sides of the luminous liquid pool is respectively provided with the A luminous liquid needle tube, the B luminous liquid needle tube and the pure water needle tube, the openings of the A luminous liquid needle tube, the B luminous liquid needle tube and the pure water needle tube are communicated with the luminous liquid pool, the A luminous liquid needle tube and the B luminous liquid needle tube are respectively externally connected with an injection pump, the pure water needle tube is externally connected with a pure water peristaltic pump, the bottom of the luminous liquid pool is provided with the waste water hole, the lower end of the waste water hole is connected with a waste water connector, the waste water connector is externally connected with the waste water peristaltic pump, the top surface of the luminous liquid pool is provided with a pool cover, the central opening of the pool cover is matched with the C chip in a shape, and the C chip is inserted in the luminous liquid pool.
Preferably, the diluent storage filling mechanism include diluent box, first cylinder, the second cylinder, the rotating electrical machines, diluent needle wash tank and second diluent needle, wherein establish two diluent in the diluent box and place the district, diluent box side and bottom surface enclose there is B heat insulating board, first cylinder is established to diluent box one side, the cylinder connecting plate is connected to the movable end of first cylinder, the diluent box is connected to cylinder connecting plate bottom surface, the diluent needle mounting panel is connected to cylinder connecting plate top surface, install two first diluent needles on the diluent needle mounting panel, two first diluent needle one end respectively with two diluent in the diluent box place the district intercommunication, two first diluent needle other ends are connected the one end of two syringe pumps through a pipeline respectively, two diluent needle joints are connected a second diluent needle respectively through a pipeline, diluent needle groove is established to second diluent needle below, diluent needle joint sets up on diluent arm crossbeam one end, the rotating electrical machines are connected through the rotating shaft motor and are connected to the rotating electrical machines and are placed the rotating electrical machines and are connected to the rotating electrical machines, the cooling fin bottom is established to the rotating electrical machines, the rotating electrical machines are connected to the rotating electrical machines, the cooling fin is connected to the rotating electrical machines, the rotating electrical machines are connected to the rotating electrical machines, the cooling fan is connected to the rotating electrical machines.
Preferably, the reaction zone temperature control mechanism comprises a heating bellows, a heating module, an air inlet fan, an air outlet fan and hot air pipelines, wherein the heating bellows is internally provided with the heating module, the air inlet fan is arranged on one side surface of the heating bellows, two hot air pipelines are arranged on the other side surface of the heating bellows opposite to the air inlet fan, the air outlet fan is arranged on the side surface of the heating bellows on the inner side of the hot air pipeline, one end of each hot air pipeline is connected with the air outlet fan, the other end of each hot air pipeline is connected with a temperature control area in the shell, a temperature probe is arranged in the temperature control area in the shell and is electrically connected with a controller, the controller is respectively electrically connected with the heating module, the air inlet fan and the air outlet fan, and shock absorption pad feet are respectively arranged on four corners of the bottom of the heating bellows.
Preferably, the chip CCD shooting mechanism comprises a servo motor, a luminous disc and a CCD, wherein a detection switch is arranged in the luminous disc, a luminous disc outer cover is arranged on the outer side of the optical disc, a luminous disc cover is arranged on the top of the luminous disc outer cover, a chip opening is formed in the luminous disc cover, a luminous disc light blocking block is arranged between the luminous disc cover and the luminous disc, the luminous disc is connected with a motor rotating flange, the motor rotating flange is arranged between the luminous disc and the luminous disc outer cover, the motor rotating flange is connected with a motor shaft, the motor shaft is connected with the servo motor, the servo motor is fixed on the bottom surface of the luminous disc outer cover through a motor mounting flange, a shooting opening is formed in the side surface of the luminous disc outer cover, a lens is aligned with the shooting opening, the lens is connected with the CCD, the CCD is mounted on a CCD mounting plate, the CCD mounting plate is mounted on a CCD base, front-back adjusting block is arranged on the CCD base, four corners of the top surface of the CCD base are provided with CCD frame columns, and a light blocking piece and a photoelectric sensor are arranged on the luminous disc outer cover on one side of the motor rotating flange.
The invention has the advantages that: the operation is convenient, after the sample, the chip and the reaction cup are placed, the instrument is started, and the actions of sample injection, code scanning, sample adding, diluent adding, serum adding, reaction, cup removing, soaking, cleaning and blow-drying, reagent adding, reaction, luminous liquid injection, blow-drying mechanism, CCD shooting and the like can be automatically performed. And finally, displaying the information of the chip on a display by the analyzer according to the photographed picture. And each liquid adding needle can be automatically cleaned, so that cross infection is prevented. The temperature control mechanism of the reaction area keeps the temperature of the analyzer constant, improves the reaction stability and accuracy, and has high efficiency.
Drawings
FIG. 1 is a schematic structural diagram of a fully automatic protein chip immunoassay instrument of the present invention.
Fig. 2-1 is a schematic diagram showing a three-dimensional structure of a chip feeding mechanism in the full-automatic protein chip immunoassay analyzer of the present invention.
Fig. 2-2 is a schematic cross-sectional structure of a chip feeding mechanism in the full-automatic protein chip immunoassay analyzer of the present invention.
Fig. 3-1 is a schematic perspective view of a chip cleaning and blow-drying mechanism in the full-automatic protein chip immunoassay analyzer of the present invention.
Fig. 3-2 is a schematic cross-sectional structure diagram of a chip cleaning and blow-drying mechanism in the full-automatic protein chip immunoassay analyzer of the present invention.
Fig. 4 is a schematic structural diagram of a feeding mechanism of a reaction cup in the full-automatic protein chip immunoassay analyzer.
FIG. 5-1 is a schematic diagram showing the three-dimensional structure of a luminescence reaction mechanism in the full-automatic protein chip immunoassay instrument of the present invention.
FIG. 5-2 is a schematic cross-sectional structural view of the mechanism of the luminescence reaction in the full-automatic protein chip immunoassay instrument of the present invention.
FIG. 6 is a schematic diagram of the diluent storage and filling mechanism in the fully automatic protein chip immunoassay analyzer of the present invention.
FIG. 7 is a schematic structural diagram of a reaction zone temperature control mechanism in a fully automatic protein chip immunoassay instrument of the present invention.
Fig. 8-1 is a schematic diagram showing a three-dimensional structure of a chip CCD photographing mechanism in the full-automatic protein chip immunoassay instrument of the present invention.
Fig. 8-2 is a schematic diagram showing a sectional structure of a chip CCD photographing mechanism in the full-automatic protein chip immunoassay instrument of the present invention.
In the figure, 1 is a shell, 201 is a cover plate, 202 is an opening and closing cover, 3 is a chip feeding mechanism, 301 is an A heat insulation plate, 302 is a supporting leg, 303 is a refrigerating module, 304 is a screw motor, 305 is a chip pushing block, 306 is a chip rack, 307 is a supporting frame, 308 is a primary sensor, 309 is a sensor light blocking block, 310 is a chip blocking sheet, 311 is an A optical fiber sensor, 312 is a chip bouncing sheet, 313 is an up-down chip bouncing cylinder, 314 is a left-right sliding rail, 315 is a chip rack left-right moving cylinder, 316 is an A chip, 4 is a chip cleaning and drying mechanism, 401 is a cleaning box, The method comprises the steps of 402 a chip placing frame, 403 a B chip, 404 a chip soaking tank, 405 a stainless steel needle tube, 406 a waste water joint, 407 a jet head, 408 a gas pipe joint, 409 a jet distance adjusting block, 5 a reaction cup feeding mechanism, 501 a linear sliding table, 502 a reaction cup vibration disk, 503 a direct vibrator, 504 a sensor mounting seat, 505 a first photoelectric sensor, 506 a fiber mounting frame, 507 a left and right driving block, 508 a tank chain mounting plate, 509 a driving bracket, 510 a driving cross beam, 511 a first stepping motor, 512 a second photoelectric sensor, The second stepper motor 513, the up-down drive mounting plate 514, the linear guide rail 515, the up-down drive block 516, the cuvette placer 517, the cylinder mounting plate 518, the holding jaw cylinder 519, the cuvette holding jaw 520, the third photosensor 521, the A light blocking plate 522, the optical fiber sensor 523, the cuvette blocking plate 524, the luminous liquid reaction mechanism 6, the luminous liquid pool 601, the A luminous liquid needle tube 602, the B luminous liquid needle tube 603, the pure water needle tube 604, the waste water hole 605, the waste water joint 606, the cell cover 607, the C chip 608, 7 is a diluent storage and fill mechanism, 701 is a diluent box, 702 is a B-plate, 703 is a first cylinder, 704 is a cylinder connection plate, 705 is a diluent needle mounting plate, 706 is a first diluent needle, 707 is a cooling fin, 708 is a radiator, 709 is a cooling fan, 710 is a second cylinder, 711 is a rotating motor, 712 is a fourth photosensor, 713 is a B-plate, 714 is a gasket, 715 is a rotating shaft tightening ring, 716 is a diluent arm cross beam, 717 is a diluent needle connector, 718 is a diluent needle wash tank, 719 is a second diluent needle, 720 is a rotating shaft, 801 is a heating bellows, 802 is a heating module, 803 is an air inlet fan, 804 is an air outlet fan, 805 is a hot air pipeline, 806 is a shock pad foot, 9 is a chip CCD photographing mechanism, 901 is a servo motor, 902 is a luminescent disk housing, 903 is a luminescent disk cover, 904 is a luminescent disk light blocking block, 905 is a luminescent disk, 906 is a motor rotating flange, 907 is a motor mounting flange, 908 is a motor shaft, 909 is a lens, 910 is a CCD, 911 is a CCD mounting plate, 912 is a CCD base, 913 is a CCD frame upright post, 914 is a front-back adjusting block, 915 is a photographing port, The reference numeral 916 denotes a chip port, 10 denotes a display and operator, 11 denotes a washing tank, 12 denotes a sample feeding mechanism, 13 denotes a serum tray, 14 denotes a reaction tray, 15 denotes a reagent tray, 16 denotes a serum arm, 17 denotes a chip arm, 18 denotes a reagent arm, 19 denotes a blow-drying mechanism, and 20 denotes a cup removing mechanism.
Detailed Description
The present invention will be described in further detail with reference to examples and embodiments.
As shown in fig. 1, the full-automatic protein chip immunity analyzer comprises a shell 1, a cover plate 201 is arranged at the top of the shell 1, an opening and closing cover 202 is arranged on the cover plate 201, a display and an operator 10 are arranged on one side of the shell 1, a serum tray 13, a reaction tray 14, a reagent tray 15 and a chip CCD shooting mechanism 9 are sequentially arranged on the cover plate 201, a chip feeding mechanism 3, a reaction cup feeding mechanism 5, a diluent storage and filling mechanism 7, a sample feeding mechanism 12 and a serum arm 16 are arranged on the outer side of the serum arm 16, a cleaning tank 11 is arranged on one side of the sample feeding mechanism 12, a scanning head is arranged on the sample feeding mechanism 12, a chip arm 17 is arranged between the serum tray 13 and the reaction tray 14, a chip cleaning and drying mechanism 4 and a cup removing mechanism 20 are arranged on one side of the chip arm 17, a reagent arm 18 is arranged between the reaction tray 14 and the reagent tray 15, a cleaning tank 11 is arranged on one side of the reagent arm 18, a chip arm 17 is arranged on one side of the reaction tray 14, a cup removing mechanism 20 and a chip cleaning mechanism 4 are arranged on one side of the chip CCD shooting mechanism 9, a chip arm 17 is arranged on one side of the chip arm 17, a chip arm 17 is arranged on one side of the reaction tray 19 and a light emitting mechanism 6 is arranged on the side of the reaction tray 1, and a garbage control region is arranged on the side of the shell.
As shown in fig. 2-1 and 2-2, the chip feeding mechanism 3 comprises an a heat insulation board 301, a screw motor 304, a chip rack 306, an original point sensor 308, an a optical fiber sensor 311, an up-down chip bouncing cylinder 313 and a chip rack left-right moving cylinder 315, wherein a supporting leg 302 is arranged at the bottom of the a heat insulation board 301, a left-right sliding rail 314 is arranged in the a heat insulation board 301, a supporting frame 307 is movably connected on the left-right sliding rail 314, the side surface of the supporting frame 307 is connected with the chip rack left-right moving cylinder 315, two mutually parallel chip racks 306 are arranged on the supporting frame 307, the chip racks 306 are limited with the supporting frame 307 through positioning pins, a chip baffle 310 is arranged in the chip racks 306, a notch matched with a notch on the a chip 316 is arranged on the chip baffle 310, an A optical fiber sensor 311 is arranged at one end of a chip frame 306, a chip bouncing piece 312 is arranged below the chip frame 306 close to the A optical fiber sensor 311, the chip bouncing piece 312 is connected with an upper chip bouncing cylinder 313 and a lower chip bouncing cylinder 313, the upper chip bouncing cylinder 313 and the lower chip bouncing cylinder 313 are arranged in an A heat insulation plate 301, a source point sensor 308 is arranged at the other end of the chip frame 306, a sensor light blocking block 309 is arranged beside the source point sensor 308, a chip pushing block 305 is arranged at the inner side of the chip frame 306 below the source point sensor 308, the chip pushing block 305 is connected with a screw rod of a screw rod motor 304, the screw rod of the screw rod motor 304 is arranged below the chip frame 306 in parallel, the screw rod motor 304 is arranged on the side wall of the A heat insulation plate 301 close to the other end of the chip frame 306, and a cooling module 303 is arranged on the side wall of the A heat insulation plate 301 at one side of the chip frame 306. The two chip frames 306 are switched in position by the chip frame left and right moving cylinder 315.
As shown in fig. 3-1 and 3-2, the chip cleaning and drying mechanism 4 comprises a cleaning box 401, a chip placing frame 402, a chip soaking tank 404 and a jet head 407, wherein the middle part in the cleaning box 401 is provided with the chip soaking tank 404, the chip placing frame 402 is arranged above the chip soaking tank 404, a chip 403 is placed on the chip placing frame 402, stainless steel needle tubes 405 are respectively arranged on tank walls on two sides of the chip soaking tank 404, the stainless steel needle tubes 405 are externally connected with cleaning liquid diaphragm pumps, liquid outlets of the stainless steel needle tubes 405 on two sides are oppositely arranged, the jet heads 407 are respectively arranged on two sides of the chip placing frame 402 on the two sides of the stainless steel needle tubes 405, the jet heads 407 are connected with air pipe joints 408, the air pipe joints 408 are externally connected with a drying device with electromagnetic valves, the waste water joints 406 are respectively connected with the bottom of the cleaning box 401 on the bottom of the chip soaking tank 404, the waste water joints 406 are connected with a diaphragm valve, the two waste water joints 406 are externally connected with the same pumping waste water diaphragm pump, the bottom of the cleaning box 401 is provided with a jet distance adjusting block 409, and the jet distance adjusting block 409 is connected with the jet heads 407 on two sides.
As shown in FIG. 4, the reaction cup feeding mechanism 5 comprises a linear sliding table 501, a reaction cup vibration disk 502, a direct vibrator 503, a left driving block 507, a right driving block 507, a linear guide rail 515, an upper driving block 516, a lower driving block 516, a reaction cup placer 517 and a reaction cup clamping jaw 520, wherein the lower part of one end of the linear sliding table 501 is provided with the reaction cup vibration disk 502 and the direct vibrator 503, the tail end of the track of the reaction cup vibration disk 502 is connected with the direct vibrator 503, the outlet of the direct vibrator 503 is provided with the reaction cup placer 517, the outer side of the reaction cup placer 517 is provided with a sensor mounting seat 504, the sensor mounting seat 504 is provided with a first photoelectric sensor 505, the side of the reaction cup placer 517 is provided with a reaction cup blocking piece 524, the outer side of the reaction cup blocking piece 524 is provided with an optical fiber mounting frame 506, the optical fiber mounting frame 506 is provided with a B optical fiber sensor, the side of the linear sliding table 501 is provided with a tank chain mounting plate 508, the inner side of the tank chain mounting plate 508 is connected with the left driving block 507 in a sliding manner, the other end of the linear sliding table 501 is provided with a first stepping motor 511 and a second photoelectric sensor 512, the first stepping motor 511 is connected with a left driving block 507 and a right driving block 507, a driving bracket 509 is arranged on the left driving block 507, a driving cross beam 510 is arranged on the driving bracket 509, a second stepping motor 513 is arranged on the driving cross beam 510, an up-down driving mounting plate 514 is arranged on the second stepping motor, a linear guide 515 is arranged on the up-down driving mounting plate 514, an up-down driving block 516 is connected on the linear guide 515 in a sliding manner, the second stepping motor 513 is connected with the up-down driving block 516, the bottom end of the up-down driving block 516 is connected with a cylinder mounting plate 518, a clamping jaw cylinder 519 is arranged on the cylinder mounting plate 518, the movable end of the clamping jaw cylinder 519 is connected with a reaction cup clamping jaw 520, a third photoelectric sensor 521 is arranged on the top end of the linear guide 515, and an A light blocking piece 522 is arranged on the top end of the up-down driving block 516.
5-1 And 5-2, the luminescence reaction mechanism 6 comprises a luminescence liquid pond 601, an A luminescence liquid needle tube 602, a B luminescence liquid needle tube 603, a pure water needle tube 604 and a waste water hole 605, wherein the top of three sides of the luminescence liquid pond 601 is respectively provided with the A luminescence liquid needle tube 602, the B luminescence liquid needle tube 603 and the pure water needle tube 604, the openings of the A luminescence liquid needle tube 602, the B luminescence liquid needle tube 603 and the pure water needle tube 604 are communicated with the luminescence liquid pond 601, the A luminescence liquid needle tube 602 and the B luminescence liquid needle tube 603 are respectively externally connected with an injection pump, the pure water needle tube 604 is externally connected with a pure water peristaltic pump, the bottom of the luminescence liquid pond 601 is provided with a waste water hole 605, the lower end of the waste water hole 605 is connected with a waste water connector 606, the waste water connector 606 is externally connected with the waste water peristaltic pump, the top surface of the luminescence liquid pond 601 is provided with a pond cover 607, the central opening of the pond cover 607 is matched with the C chip 608 in shape, and the C chip 608 is inserted in the center of the pond cover 607 and the lower part of the C chip 608 is positioned in the luminescence liquid pond 601.
As shown in FIG. 6, the diluent storage and filling mechanism comprises a diluent box 701, a first cylinder 703, a second cylinder 710, a rotating motor 711, diluent needle washing grooves 718 and a second diluent needle 719, wherein two diluent placing areas are arranged in the diluent box 701, a B heat insulation plate 702 is surrounded on the side surface and the bottom surface of the diluent box 701, the first cylinder 703 is arranged on one side of the diluent box 701, the movable end of the first cylinder 703 is connected with a cylinder connecting plate 704, the bottom surface of the cylinder connecting plate 704 is connected with the diluent box 701, the top surface of the cylinder connecting plate 704 is connected with a diluent needle mounting plate 705, two first diluent needles 706 are mounted on the diluent needle mounting plate 705, one ends of the two first diluent needles 706 are respectively communicated with the two diluent placing areas in the diluent box 701, the other ends of the two first diluent needles 706 are respectively connected with one ends of two injection pumps (not shown) through a pipeline, the other ends of the two injection pumps are respectively connected with two diluent needle joints 717 through a pipeline, the two diluent needle joints 717 are respectively connected with a second diluent needle 719, the movable end of the first diluent needle is connected with a motor 719, the bottom surface of the second diluent needle is connected with the bottom surface of the second diluent box 704 is connected with the second diluent motor 714, the top surface is connected with the rotating motor 716, the rotating shaft is connected with the rotating shaft 708 through a rotating shaft 708, the rotating shaft is connected with the rotating shaft 708 is arranged at one side of the rotating shaft through the rotating shaft 708, and the rotating shaft is connected with the rotating shaft 708, and is connected with the rotating shaft 708.
As shown in fig. 7, the reaction zone temperature control mechanism includes a heating bellows 801, a heating module 802, an air inlet fan 803, an air outlet fan 804 and a hot air pipeline 805, wherein the heating bellows 801 is internally provided with the heating module 802, the air inlet fan 803 is arranged on one side surface of the heating bellows 801, two hot air pipelines 805 are arranged on the other side surface of the heating bellows 801 opposite to the air inlet fan 803, the air outlet fan 804 is arranged on the side surface of the heating bellows 801 inside the hot air pipeline 805, one end of the two hot air pipelines 805 is connected with the air outlet fan 804, the other end of the two hot air pipelines 805 is connected with a temperature control area in the shell 1, a temperature probe (not shown) is arranged in the temperature control area in the shell 1, the temperature probe is electrically connected with a controller (not shown), the controller is respectively electrically connected with the heating module 802, the air inlet fan 803 and the air outlet fan 804, and shock pad feet 806 are respectively arranged on four corners at the bottom of the heating bellows 801.
As shown in fig. 8-1 and 8-2, the chip CCD shooting mechanism 9 includes a servo motor 901, a luminescent disc 905 and a CCD910, wherein a detection switch is provided in the luminescent disc 905, a luminescent disc housing 902 is provided outside the optical disc 905, a luminescent disc cover 903 is provided on the top of the luminescent disc housing 902, a chip opening 916 is provided on the luminescent disc cover 903, a luminescent disc light blocking block 904 is provided between the luminescent disc cover 903 and the luminescent disc 905, the luminescent disc 905 is connected with a motor rotating flange 906, the motor rotating flange 906 is provided between the luminescent disc 905 and the luminescent disc housing 902, the motor rotating flange 906 is connected with a motor shaft 908, the motor shaft 908 is connected with the servo motor 901, the servo motor 901 is fixed on the bottom of the luminescent disc housing 902 through a motor mounting flange 907, a shooting opening 915 is provided on the side of the luminescent disc housing 902, a lens 909 is aligned with the shooting opening 915, the lens is connected with the CCD910, the CCD910 is mounted on the CCD mounting plate 911, the CCD mounting plate 911 is mounted on a CCD base 912, front and rear adjusting blocks 914 are provided on four corners of the top of the CCD base 912, the luminescent disc housing 902 is provided with a light blocking plate and a light sensor on one side of the motor rotating flange 906.
The cleaning tank 11, the sample feeding mechanism 12, the serum tray 13, the reaction tray 14, the reagent tray 15, the serum arm 16, the chip arm 17, the reagent arm 18, the blow-drying mechanism 19 and the cup removing mechanism 20 are all of the prior art, any structure capable of realizing the functions can be used, the cleaning tank 11 is used for cleaning the liquid feeding needles on the serum arm 16 and the reagent arm 18, the sample feeding mechanism 12 is used for placing and pushing samples, the serum tray 13, the reaction tray 14 and the reagent tray 15 can rotate and place reaction cups, the serum arm 16 is used for filling serum into the reaction cups, the chip arm 17 is used for clamping and placing chips, the reagent arm 18 is used for filling reagents into the reaction cups, the blow-drying mechanism 19 is used for blow-drying the chips, and the cup removing mechanism 20 is used for placing waste reaction cups.
The a chip 316, B chip 403, C chip 608 are shown with the same chip in different positions.
According to the above structure, when in operation, firstly, a sample is placed in the sample injection mechanism 12, the diluent and the luminous liquid are respectively placed in the corresponding positions of the diluent storage filling mechanism 7 and the luminous liquid container connected with the luminous liquid reaction mechanism 6, all liquid filling pipelines are controlled, the reaction cup feeding mechanism 5 is internally provided with a reaction cup, the chip rack of the chip feeding mechanism 3 is placed, and the corresponding water pipes are inserted into the corresponding pure water barrel (connected with a pure water peristaltic pump or a diaphragm pump), the washing liquid barrel (connected with a washing liquid diaphragm pump) and the corresponding wastewater barrel (connected with a wastewater pumping peristaltic pump or a diaphragm pump) which are arranged outside the instrument. And then controlling the origin regression of the analyzer, and starting the analyzer after the origin regression is finished. The sampling mechanism pushes 12 samples to the position, the scanning head scans the corresponding bar code, the serum arm 16 rotates to enable the sample adding needle to absorb the samples, the reaction cup feeding mechanism 5 detects the reaction cup, the clamping jaw grabs the reaction cup and places the reaction cup at the corresponding position of the serum tray 13, the serum tray 13 rotates to the diluent storage and filling mechanism 7 to add diluent, the serum tray 13 rotates to the sampling mechanism 12 to add samples, the serum arm 16 rotates to the cleaning tank 11 to clean the sample adding needle to prevent cross contamination, the serum tray 13 rotates to the chip adding position to add serum, the serum tray 13 rotates to the chip feeding mechanism 3 again, the chip feeding mechanism 3 bounces the chip, the chip arm 17 grabs the chip and places the chip into the reaction cup, and timing is started for 40 minutes immediately. After 40 minutes of reaction, the reaction cup is grabbed by the chip arm 17 and put into the cup removing mechanism 20, the reaction cup is thrown away, and then the chip is put into the chip cleaning and drying mechanism 4 for soaking, cleaning and drying for 2 minutes. The reaction disk 14 is rotated to the reagent arm 18, the reagent arm 18 sucks the reagent from the reagent disk 15 and injects the reagent into the reaction cup, then the reagent arm 18 is rotated to the cleaning tank 11 to clean the reagent needle, the reaction disk 14 is rotated to rotate the reaction cup to the corresponding chip arm 17, and the chip arm 17 puts the cleaned chip into the reaction cup to react for 30 minutes. After 30 minutes, the chip arm 17 grabs the reaction cup and puts the reaction cup into the cup removing mechanism 20 to throw away the reaction cup, and then puts the chip into the cleaning and drying mechanism 4 to soak, clean and dry for 2 minutes. After 2 minutes, the luminous liquid is injected into the luminous liquid reaction mechanism 6, the chip arm 17 puts the cleaned chip into the luminous reaction mechanism 6 for soaking for 30 seconds, then the chip arm 17 grabs the chip to the blow-drying mechanism 19, the water mark on the surface of the chip is blow-dried, the chip is put into the luminous disc of the chip CCD shooting mechanism 9, the luminous disc is rotated to the shooting position, the CCD shoots for 30 seconds, the luminous disc rotates again, and the chip arm 17 grabs the chip and throws the chip into the garbage can. And the analyzer displays the information of the chip on a display according to the photographed picture.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that modifications and improvements could be made by those skilled in the art without departing from the inventive concept, which falls within the scope of the present invention.
Claims (1)
1. The full-automatic protein chip immunity analyzer is characterized by comprising a shell (1), wherein a cover plate (201) is arranged at the top of the shell (1), an opening and closing cover (202) is arranged on the cover plate (201), a display and an operator (10) are arranged on one side of the shell (1), a serum tray (13), a reaction tray (14), a reagent tray (15) and a chip CCD shooting mechanism (9) are sequentially arranged on the cover plate (201), a chip feeding mechanism (3), a reaction cup feeding mechanism (5), a diluent storage and filling mechanism (7), a sample injection mechanism (12) and a serum arm (16) are arranged on the outer side of the surrounding serum tray (13), a cleaning tank (11) is arranged on one side of the serum arm (16), A sample feeding mechanism (12) is provided with a sample scanning head, a chip arm (17) is arranged between a serum tray (13) and a reaction tray (14), a chip cleaning and drying mechanism (4) and a cup removing mechanism (20) are arranged on one side of the chip arm (17), a reagent arm (18) is arranged between the reaction tray (14) and a reagent tray (15), a cleaning tank (11) is arranged on one side of the reagent arm (18), a chip arm (17) is arranged on one side of the reaction tray (14), a cup removing mechanism (20) and a chip cleaning and drying mechanism (4) are arranged on one side of the chip arm (17), a chip arm (17) is arranged on one side of a chip CCD shooting mechanism (9), A blow-drying mechanism (19), a luminous liquid reaction mechanism (6) and a garbage box are arranged on one side of a chip arm (17), a reaction area temperature control mechanism is arranged on the side face of a shell (1), a cleaning tank (11) is used for cleaning a liquid adding needle on a serum arm (16) and a reagent arm (18), a sample feeding mechanism (12) is used for placing and pushing samples, a serum tray (13), a reaction tray (14) and a reagent tray (15) are all rotatable and are used for placing a reaction cup, the serum arm (16) is used for filling serum into the reaction cup, the chip arm (17) is used for clamping and placing chips, the reagent arm (18) is used for filling reagent into the reaction cup, the blow-drying mechanism (19) is used for blow-drying the chips, the cup removing mechanism (20) is used for placing the waste reaction cup; The chip feeding mechanism (3) comprises an A heat insulation plate (301), a screw motor (304), a chip rack (306), an original point sensor (308), an A optical fiber sensor (311), an upper chip bouncing cylinder (313) and a lower chip bouncing cylinder (315) and a chip rack left and right moving cylinder (315), wherein a supporting leg (302) is arranged at the bottom of the A heat insulation plate (301), a left and right sliding rail (314) is arranged in the A heat insulation plate (301), a supporting frame (307) is movably connected on the left and right sliding rail (314), the side surface of the supporting frame (307) is connected with the chip rack left and right moving cylinder (315), two mutually parallel chip racks (306) are arranged on the supporting frame (307), Chip rack (306) is limited with support frame (307) by locating pin, chip baffle (310) is arranged in chip rack (306), a gap matched with the gap on A chip (316) is arranged on chip baffle (310), A optical fiber sensor (311) is arranged at one end of chip rack (306), chip bouncing piece (312) is arranged under chip rack (306) close to A optical fiber sensor (311), chip bouncing piece (312) is connected with upper and lower chip bouncing air cylinders (313), upper and lower chip bouncing air cylinders (313) are arranged in A heat insulation board (301), original point sensor (308) is arranged at the other end of chip rack (306), A sensor light blocking block (309) is arranged beside an original point sensor (308), a chip pushing block (305) is arranged on the inner side of a chip frame (306) below the original point sensor (308), the chip pushing block (305) is connected with a screw rod of a screw rod motor (304), the screw rod of the screw rod motor (304) is arranged below the chip frame (306) in parallel, the screw rod motor (304) is arranged on the side wall of an A heat insulation plate (301) close to the other end of the chip frame (306), and a refrigeration module (303) is arranged on the side wall of the A heat insulation plate (301) on one side of the chip frame (306); The chip cleaning and drying mechanism (4) comprises a cleaning box (401), a chip placing frame (402), a chip soaking tank (404) and a jet head (407), wherein the chip soaking tank (404) is arranged in the middle of the cleaning box (401), the chip placing frame (402) is arranged above the chip soaking tank (404), a B chip (403) is arranged on the chip placing frame (402), stainless steel needle tubes (405) are respectively arranged on tank walls on two sides of the chip soaking tank (404), the stainless steel needle tubes (405) are externally connected with a cleaning liquid diaphragm pump, liquid outlets of the stainless steel needle tubes (405) on two sides are oppositely arranged, the jet heads (407) are respectively arranged on two sides of the chip placing frame (402) above the stainless steel needle tubes (405) on two sides, the air jet head (407) is connected with an air pipe joint (408), the air pipe joint (408) is externally connected with a blow-drying device with an electromagnetic valve, the bottom of the chip soaking tank (404) and the bottom of the cleaning box (401) at the outer side of the bottom of the chip soaking tank (404) are respectively connected with a first waste water joint (406), the first waste water joint (406) at the bottom of the chip soaking tank (404) is connected with a diaphragm valve, the two first waste water joints (406) are externally connected with the same waste water pumping diaphragm pump, the bottom of the cleaning box (401) is provided with an air jet distance adjusting block (409), and the air jet distance adjusting block (409) is connected with the two air jet heads (407); The reaction cup feeding mechanism (5) comprises a linear sliding table (501), a reaction cup vibration disc (502), a direct vibrator (503), a left driving block and a right driving block (507), a linear guide rail (515), an upper driving block and a lower driving block (516), a reaction cup placer (517) and a reaction cup clamping jaw (520), wherein the reaction cup vibration disc (502) and the direct vibrator (503) are arranged below one end of the linear sliding table (501), the track tail end of the reaction cup vibration disc (502) is connected with the direct vibrator (503), the outlet of the direct vibrator (503) is provided with the reaction cup placer (517), the outer side of the reaction cup placer (517) is provided with a sensor mounting seat (504), A first photoelectric sensor (505) is arranged on a sensor mounting seat (504), a reaction cup baffle (524) is arranged beside a reaction cup placer (517), an optical fiber mounting frame (506) is arranged on the outer side of the reaction cup baffle (524), a B optical fiber sensor is arranged on the optical fiber mounting frame (506), a tank chain mounting plate (508) is arranged on the side surface of a linear sliding table (501), a left and right driving block (507) is slidingly connected on the linear sliding table (501) on the inner side of the tank chain mounting plate (508), a first stepping motor (511) and a second photoelectric sensor (512) are arranged on the other end of the linear sliding table (501), the first stepping motor (511) is connected with the left and right driving block (507), A driving bracket (509) is arranged on the left driving block (507), a driving cross beam (510) is arranged on the driving bracket (509), a second stepping motor (513) is arranged on the driving cross beam (510), an upper and lower driving mounting plate (514) is arranged on the second stepping motor, a linear guide rail (515) is arranged on the upper and lower driving mounting plate (514), the upper and lower driving block (516) is connected on the linear guide rail (515) in a sliding manner, the second stepping motor (513) is connected with the upper and lower driving block (516), the bottom end of the upper and lower driving block (516) is connected with an air cylinder mounting plate (518), a clamping jaw air cylinder (519) is arranged on the air cylinder mounting plate (518), The movable end of the clamping jaw cylinder (519) is connected with a reaction cup clamping jaw (520), the top end of the linear guide rail (515) is provided with a third photoelectric sensor (521), and the top end of the upper and lower driving block (516) is provided with an A light blocking sheet (522); The luminous liquid reaction mechanism (6) comprises a luminous liquid pool (601), an A luminous liquid needle tube (602), a B luminous liquid needle tube (603), a pure water needle tube (604) and a waste water hole (605), wherein the top of three sides of the luminous liquid pool (601) are respectively provided with the A luminous liquid needle tube (602), the B luminous liquid needle tube (603) and the pure water needle tube (604), the openings of the A luminous liquid needle tube (602), the B luminous liquid needle tube (603) and the pure water needle tube (604) are communicated with the luminous liquid pool (601), the A luminous liquid needle tube (602) and the B luminous liquid needle tube (603) are respectively externally connected with a syringe pump, The pure water needle tube (604) is externally connected with a pure water peristaltic pump, a waste water hole (605) is formed in the bottom of the luminous liquid pool (601), the lower end of the waste water hole (605) is connected with a second waste water connector (606), the second waste water connector (606) is externally connected with the waste water peristaltic pump, the top surface of the luminous liquid pool (601) is provided with a pool cover (607), the central opening of the pool cover (607) is matched with the C chip (608) in shape, the C chip (608) is inserted in the center of the pool cover (607), and the lower part of the C chip (608) is positioned in the luminous liquid pool (601); The diluent storage filling mechanism comprises a diluent box (701), a first air cylinder (703), a second air cylinder (710), a rotating motor (711), a diluent needle washing groove (718) and a second diluent needle (719), wherein two diluent placing areas are arranged in the diluent box (701), a B heat insulation plate (702) is enclosed on the side surface and the bottom surface of the diluent box (701), the first air cylinder (703) is arranged on one side of the diluent box (701), the movable end of the first air cylinder (703) is connected with an air cylinder connecting plate (704), the bottom surface of the air cylinder connecting plate (704) is connected with the diluent box (701), the top surface of the air cylinder connecting plate (704) is connected with a diluent needle mounting plate (705), two first diluent needles (706) are arranged on the diluent needle mounting plate (705), one ends of the two first diluent needles (706) are respectively communicated with two diluent placing areas in the diluent box (701), the other ends of the two first diluent needles (706) are respectively connected with one ends of two injection pumps through a pipeline, the other ends of the two injection pumps are respectively connected with two diluent needle joints (717) through a pipeline, the two diluent needle joints (717) are respectively connected with a second diluent needle (719), a diluent needle washing groove (718) is arranged below the second diluent needle (719), the diluent needle joints (717) are arranged on one end of a diluent arm beam (716), The other end of the diluent arm cross beam (716) is connected with the top end of a rotating shaft (720) through a rotating shaft tightening ring (715), the bottom end of the rotating shaft (720) is connected with the end part of the rotating shaft of a rotating motor (711) through a gasket (714), a B light blocking sheet (713) is arranged on the rotating shaft of the rotating motor (711), a fourth photoelectric sensor (712) is arranged on one side of the rotating shaft of the rotating motor (711), the bottom of the rotating motor (711) is connected with a second cylinder (710), a refrigerating sheet (707) is arranged on one side of the B heat blocking sheet (702), the refrigerating sheet (707) is connected with a radiator (708), and the radiator (708) is connected with a cooling fan (709); the reaction zone temperature control mechanism comprises a heating bellows (801), a heating module (802), an air inlet fan (803), an air outlet fan (804) and a hot air pipeline (805), wherein the heating bellows (801) is internally provided with the heating module (802), one side surface of the heating bellows (801) is provided with the air inlet fan (803), the other side surface of the heating bellows (801) opposite to the air inlet fan (803) is provided with two hot air pipelines (805), the side surface of the heating bellows (801) at the inner side of the hot air pipeline (805) is provided with the air outlet fan (804), one end of the two hot air pipelines (805) is connected with the air outlet fan (804), The other ends of the two hot air pipelines (805) are connected with a temperature control area in the shell (1), a temperature probe is arranged in the temperature control area in the shell (1), the temperature probe is electrically connected with a controller, the controller is respectively electrically connected with a heating module (802), an air inlet fan (803) and an air outlet fan (804), and damping pad feet (806) are respectively arranged at four corners of the bottom of the heating bellows (801); The chip CCD shooting mechanism (9) comprises a servo motor (901), a luminescent disk (905) and a CCD (910), wherein a detection switch is arranged in the luminescent disk (905), a luminescent disk outer cover (902) is arranged on the outer side of the luminescent disk (905), a luminescent disk cover (903) is arranged on the top of the luminescent disk outer cover (902), a chip opening (916) is arranged on the luminescent disk cover (903), a luminescent disk light blocking block (904) is arranged between the luminescent disk cover (903) and the luminescent disk (905), the luminescent disk (905) is connected with a motor rotary flange (906), the motor rotary flange (906) is arranged between the luminescent disk (905) and the luminescent disk outer cover (902), The motor rotating flange (906) is connected with a motor shaft (908), the motor shaft (908) is connected with a servo motor (901), the servo motor (901) is fixed on the bottom surface of a luminous disc housing (902) through a motor mounting flange (907), a shooting opening (915) is arranged on the side surface of the luminous disc housing (902), a lens (909) is aligned with the shooting opening (915), the lens (909) is connected with a CCD (910), the CCD (910) is arranged on a CCD mounting plate (911), the CCD mounting plate (911) is arranged on a CCD base (912), a front-back regulating block (914) is arranged on the CCD base (912), CCD frame posts (913) are arranged at four corners of the top surface of the CCD base (912), and a light blocking sheet and a photoelectric sensor are arranged on a luminous disc outer cover (902) at one side of a motor rotating flange (906).
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| CN201810716256.0A CN108646039B (en) | 2018-07-03 | 2018-07-03 | Full-automatic protein chip immunity analyzer |
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| CN201810716256.0A CN108646039B (en) | 2018-07-03 | 2018-07-03 | Full-automatic protein chip immunity analyzer |
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| CN108646039B true CN108646039B (en) | 2024-09-03 |
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| CN109374905A (en) * | 2018-12-27 | 2019-02-22 | 郑州航空港区艾科寿生物科技有限公司 | A kind of device based on isothiocyano label monoclonal antibody |
| CN110361553B (en) * | 2019-07-22 | 2023-05-23 | 迈克医疗电子有限公司 | Full-automatic sample analyzer and needle washing method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101696971A (en) * | 2009-10-19 | 2010-04-21 | 深港产学研基地 | Biochip analyzer and control system thereof |
| CN201600327U (en) * | 2009-12-31 | 2010-10-06 | 上海丰汇医用仪器有限公司 | Equivalent luminous liquid sample adding system of full automatic liquid-phase chemiluminescent immunoassay analyzer |
| CN102814294A (en) * | 2012-06-19 | 2012-12-12 | 江苏三联生物工程有限公司 | Cleaning and drying device for biological chip |
| CN102998473A (en) * | 2012-12-19 | 2013-03-27 | 北京利德曼生化股份有限公司 | Full-automatic chemiluminescence immune analyzer |
| CN206557225U (en) * | 2017-03-19 | 2017-10-13 | 武汉旅航科技有限公司 | A kind of incubation heater of efficient immune quantitative tacheometer |
| CN206827559U (en) * | 2017-05-17 | 2018-01-02 | 中山市松联电子科技有限公司 | A kind of computer memory bar connector automatic feeding |
| CN207067155U (en) * | 2017-07-10 | 2018-03-02 | 博奥赛斯（天津）生物科技有限公司 | A kind of reaction cup automatic pick-and-place apparatus of chemical illumination immunity analysis instrument |
| CN208383902U (en) * | 2018-07-03 | 2019-01-15 | 无锡国盛生物工程有限公司 | Full-automatic protein chip immunity analysis instrument |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1873021A (en) * | 2005-06-01 | 2006-12-06 | 上海棱光技术有限公司 | Working platform of low-density biochip capable of polynary tests in one-off |
| CN203232038U (en) * | 2013-05-10 | 2013-10-09 | 无锡国盛生物工程有限公司 | Full automatic chemiluminiscence biochip scanner |
| CN104535782B (en) * | 2014-12-29 | 2017-01-25 | 基蛋生物科技股份有限公司 | Full-automatic immuno-fluorescence quantitative analysis device and implementation method |
| CN207215839U (en) * | 2017-09-29 | 2018-04-10 | 北京理工大学 | A kind of protein detection device based on micro-fluidic chip |
-
2018
- 2018-07-03 CN CN201810716256.0A patent/CN108646039B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101696971A (en) * | 2009-10-19 | 2010-04-21 | 深港产学研基地 | Biochip analyzer and control system thereof |
| CN201600327U (en) * | 2009-12-31 | 2010-10-06 | 上海丰汇医用仪器有限公司 | Equivalent luminous liquid sample adding system of full automatic liquid-phase chemiluminescent immunoassay analyzer |
| CN102814294A (en) * | 2012-06-19 | 2012-12-12 | 江苏三联生物工程有限公司 | Cleaning and drying device for biological chip |
| CN102998473A (en) * | 2012-12-19 | 2013-03-27 | 北京利德曼生化股份有限公司 | Full-automatic chemiluminescence immune analyzer |
| CN206557225U (en) * | 2017-03-19 | 2017-10-13 | 武汉旅航科技有限公司 | A kind of incubation heater of efficient immune quantitative tacheometer |
| CN206827559U (en) * | 2017-05-17 | 2018-01-02 | 中山市松联电子科技有限公司 | A kind of computer memory bar connector automatic feeding |
| CN207067155U (en) * | 2017-07-10 | 2018-03-02 | 博奥赛斯（天津）生物科技有限公司 | A kind of reaction cup automatic pick-and-place apparatus of chemical illumination immunity analysis instrument |
| CN208383902U (en) * | 2018-07-03 | 2019-01-15 | 无锡国盛生物工程有限公司 | Full-automatic protein chip immunity analysis instrument |
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