JP4294393B2 - Antiseptic disinfectant and cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant - Google Patents

Description

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【００２６】
【発明の効果】
以上詳述した如く、本発明の防腐殺菌剤は、１，２−アルカンジオールが本来有する抗菌力を増強する効果を奏する。よって、従来の防腐殺菌剤を配合する必要がなくなり、高い安全性を得ることもできる。
また、本発明の防腐殺菌剤を配合した化粧料、医薬品及び食品は、パラベン、安息香酸、サリチル酸等の従来の防腐剤を含有する必要がなく、しかも本発明の防腐殺菌剤は優れた増強された抗菌活性を有しているので、防腐殺菌剤自体を低配合量とすることができ、極めて安全性の高いものである。
【図面の簡単な説明】
【図１】実施例１の二元最小発育阻止濃度図である。
【図２】二元最小発育阻止濃度から、抗菌性を有する二種類の物質を配合した場合により生じる作用効果を判定する方法の一例を示す図である。
【図３】実施例２の二元最小発育阻止濃度図である。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to antiseptic fungicides and cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant, the purpose of which is by blending a C1-C10 1,2-alkanediol and a specific perfume ingredient, An object of the present invention is to provide an antiseptic and bactericidal agent that enhances the antibacterial activity inherent to 1,2-alkanediol, and cosmetics, pharmaceuticals, and foods containing the antiseptic and bactericidal agent.
[0002]
[Prior art]
Parabens, benzoic acids, salicylic acids, and the like are used as antiseptic fungicides in cosmetics (including quasi drugs), pharmaceuticals, and foods. However, the above-mentioned conventional antiseptic disinfectant has a drawback that the concentration range in use is easily limited because of low safety such as high skin irritation. For example, the use restriction concentration of paraben and benzoate is 1%, and the use restriction concentration of benzoic acid and salicylic acid is 0.2%. In addition, since these antiseptic fungicides are easily affected by pH, the stability of the antiseptic disinfection effect is poor, and the antibacterial antibacterial effect may be significantly reduced when used in combination with other components such as surfactants. Also had. In recent years, the number of people who cause allergic reactions to these antiseptics has increased, so consumers are more likely to be safe and have no or no preservatives. There is a growing demand for cosmetics, pharmaceuticals and foods.
[0003]
As a technique for reducing or eliminating the antiseptic disinfectant, an antiseptic disinfectant composed of 1,2-alkanediol (see Patent Document 1), and a moisturizing bacteriostatic agent for cleansing or non-detergent cosmetics composed of 1,2-octanediol An agent (see Patent Document 2) is disclosed. However, when a 1,2-alkanediol such as 1,2-octanediol is used alone as an antiseptic disinfectant, a high blending amount may be required in the presence of a nonionic surfactant. Moreover, since 1,2-alkanediol has a peculiar raw material odor, it has a problem of odor when blended in cosmetics.
[0004]
Moreover, as a technique related to an antiseptic fungicide using 1,2-alkanediol, an external composition (see Patent Document 3) in which 1,2-pentanediol and 2-phenoxyethanol are combined and 1,2-alkanediol is used. And an antiseptic disinfectant composed of paraben (see Patent Document 4). However, these are intended to improve the effect of the preservative by using a preservative and a 1,2-alkanediol such as 1,2-pentanediol in an attempt to completely eliminate the preservative itself. It is not intended to enhance the antibacterial activity of 1,2-alkanediol.
[0005]
[Patent Document 1]
Japanese Patent Laid-Open No. 11-322591 [Patent Document 2]
JP 2001-48720 A [Patent Document 3]
Japanese Patent Laid-Open No. 10-53510 [Patent Document 4]
Japanese Patent Laid-Open No. 11-310506
[Problems to be solved by the invention]
In view of this situation, as a result of intensive studies by the present inventors, when 1,2-alkanediol and a specific perfume ingredient are used together, the antibacterial power inherent to 1,2-alkanediol is enhanced and 1 , 2-Alkanediol has been found to mask the raw material odor, and the present invention has been completed.
[0007]
[Means for Solving the Problems]
That is, the invention according to claim 1 relates to an antiseptic disinfectant characterized by containing a 1,2-alkanediol having 5 to 10 carbon atoms and isobornyl acetate and / or guaiac acetate.
The invention according to claim 2 relates to the antiseptic fungicide according to claim 1, wherein the 1,2-alkanediol is 1,2-hexanediol and / or 1,2-octanediol.
The invention according to claim 3 relates to the antiseptic fungicide according to claim 1, wherein the 1,2-alkanediol is 1,2-octanediol.
According to claim 4 the invention also relates to a cosmetic which is characterized in that blended according antiseptic disinfectant to any one of claims 1 to 3 as an antiseptic disinfectant.
The invention which concerns on Claim 5 is related with the pharmaceutical characterized by mix | blending the antiseptic disinfectant in any one of Claims 1-3 as an antiseptic disinfectant.
The invention which concerns on Claim 6 is related with the foodstuff which mix | blended the antiseptic disinfectant in any one of Claims 1-3 as an antiseptic disinfectant.
[0008]
DETAILED DESCRIPTION OF THE INVENTION
The antiseptic disinfectant according to the present invention contains 1,2-alkanediol and isobornyl acetate and / or guaiac acetate as active ingredients.
[0009]
The first component according to the present invention is 1,2-alkanediol having a linear alkyl chain having 5 to 10 carbon atoms, specifically 1,2-pentanediol, 1,2-hexane. Diol, 1,2-heptanediol, 1,2-octanediol, 1,2-nonanediol, and 1,2-decanediol. Of these, 1,2-hexanediol and 1,2-octanediol are preferably used, and 1,2-octanediol is more preferably used. In addition, in this invention, these 1 type can also be used individually or in combination of 2 or more types.
[0010]
The second component of the antiseptic disinfectant according to the present invention is isobornyl acetate and / or guaiac acetate.
Isobornyl acetate is a colorless to pale yellow liquid that is naturally present in needle oil, eucalyptus oil, etc., and can also be synthesized by reacting camphene with acetic acid in the presence of sulfuric acid.
Guayac acetate (also known as guayl acetate) is a colorless to pale yellow liquid obtained by acetylation of Guaiac wood oil.
[0011]
In the antiseptic disinfectant according to the present invention, the content of the first component of C1-C10 1,2-alkanediol and the second component of the perfume component is not particularly limited, but the weight ratio is 0. .1: 1 to 100: 1, preferably 0.2: 1 to 5: 1. When 1,2-alkanediol is blended in excess of 100 times the content of the fragrance component, an antibacterial activity enhancing effect cannot be expected. It is not preferable because it becomes too strong.
[0012]
The antiseptic disinfectant according to the present invention contains a C1-C10 1,2-alkanediol as the first component and a perfume component isobornyl acetate and / or guaiac acetate as the second component. In addition to masking the raw material odor of 1,2-alkanediol, the perfume component has an excellent antibacterial activity due to the synergistic effect of the first component and the second component, as shown in the test described below. Demonstrated. Therefore, it is not necessary to blend or blend conventionally used antiseptics such as parabens, benzoic acids, and salicylic acids, and an extremely safe antiseptic can be obtained.
[0013]
The antiseptic disinfectant of the present invention can be used by blending it in cosmetics, pharmaceuticals, foods and the like. Specifically, facial cleanser, lotion, milky lotion, skin cream, foundation, mascara, nail enamel, lipstick and other skin cosmetics, shampoo, hair treatment, hair growth / hair restorer, hair cream, hair foam, permanent wave agent, etc. For cosmetics for specific hair use such as cosmetics for hair, freckles and other medicinal cosmetics (quasi-drugs), acne medicines, gargles, lozenges, chewing gum, candy, drinking water and other foods It can be used suitably.
[0014]
When preparing cosmetics, pharmaceuticals or foods using the antiseptic disinfectant of the present invention, the components usually used in cosmetics, pharmaceuticals or foods are optionally arbitrarily blended as long as the effects of the present invention are not impaired. be able to. For example, in the case of cosmetics (including quasi-drugs) and pharmaceuticals, fats and oils, waxes, higher fatty acids, lower alcohols, higher alcohols, sterols, fatty acid esters, humectants, surfactants, polymer compounds, inorganic pigments , Pigments, antioxidants, ultraviolet absorbers, vitamins, astringents, whitening agents, animal and plant extracts, sequestering agents, purified water, and the like. In the case of food, animal and vegetable oils, polysaccharides, sweeteners, colorants, gum bases and the like can be exemplified.
[0015]
When the antiseptic disinfectant of the present invention is blended in cosmetics, pharmaceuticals or foods, the blending amount is not particularly limited, but is 0.01 to 20% by weight, preferably 0.05 to 5% by weight in the composition. This is because when the amount is less than 0.01% by weight, the antibacterial effect is inferior, and even if the amount exceeds 20% by weight, no further effect can be expected.
[0016]
【Example】
Example 1
(Co-test)
Staphylococcus aureus IFO13276 (Staphylococcus aureus) was used as a co-test fungus.
[0017]
(Preparation of bacterial solution for inoculation)
As a bacterial solution for inoculation, after culturing at 35 ° C. on an agar medium, it was further transplanted to bouillon medium and cultured at 35 ° C. A solution obtained by diluting the obtained culture solution to about 10 ⁸ cells / ml with a bouillon medium was used as a bacterial solution for inoculation.
[0018]
(Preparation of dilution series of test substance)
A 20 w / w% ethyl cellosolve was used as a diluent solvent to prepare a 5 w / v% 1,2-octanediol liquid (mother liquid). This mother liquor was diluted twice to prepare a 10-stage dilution series. Similarly, 10-step dilution series were prepared for isobornyl acetate and a mixture of equal amounts of 1,2-octanediol and isobornyl acetate.
[0019]
(Measurement of minimum inhibitory concentration (MIC))
Each agar medium to which a dilution series containing the test substance was added was placed in a petri dish, and the inoculum for the inoculum was streaked to a length of about 1 cm. The culture was performed at 35 ° C., and the presence or absence of growth of the bacteria after 2 days was determined. At this time, the minimum concentration at which no growth was observed was determined as MIC. As a result, 1,2-octanediol was 2250 μg / ml, isobornyl acetate was 5000 μg / ml, and an equal weight mixture of 1,2-octanediol and isobornyl acetate was 2500 μg / ml.
In addition, antibacterial power can be evaluated by MIC. When the test substance concentration is low, there is no effect on microorganisms, but growth increases when the concentration is increased. This degree of growth proceeds depending on the concentration, and finally the growth stops. The concentration at this time is expressed as MIC. Therefore, when the concentration is higher than MIC, the microorganisms are killed.
[0020]
(Dual minimum inhibitory concentration)
Each MIC of the resulting 1,2-octanediol, isobornyl acetate, and an equal weight mixture of 1,2-octanediol and isobornyl acetate was plotted against the blending amount of 1,2-octanediol and isobornyl acetate, A binary minimum inhibitory concentration diagram was determined. The results are shown in FIG.
In addition, the effect at the time of mix | blending two types of substances which have antimicrobial property can be determined with a binary minimum growth inhibitory density | concentration. Specifically, when two types of substances having antibacterial properties are blended, the action caused by the substances is roughly classified into a synergistic action, an additive action, and an antagonistic action. The synergistic action is an action in which two drugs act synergistically to further enhance the inherent antibacterial power. The additive action is an action in which the antibacterial powers of the respective drugs are combined. Antagonism is an action when one drug counteracts the antibacterial activity of another drug. The method based on the binary minimum growth inhibition concentration diagram is, for example, a method of determining from the graph by measuring the MIC for each of the A substance and B substance while changing their ratios as shown in FIG. According to this, when MIC (point A) in only substance A and MIC (point B) only in substance B are connected, the MIC when both substances are used is on the inner side of this line (point C) is a synergistic effect in which the antibacterial activity is enhanced by the combined use, if it is on the line (point D), if it is an additive action, if it is outside the line (point E), either or both It can be determined that the antagonistic action counteracts the antibacterial activity and decreases the antibacterial activity.
[0021]
(Evaluation of antibacterial effect)
From the result of FIG. 1, the synergistic effect of antibacterial activity was recognized by the combination of 1,2-octanediol and isobornyl acetate.
[0022]
Example 2
For guaiac acetate, MIC was determined in the same manner as in Example 1 above. As a result, 1,2-octanediol was 2250 μg / ml, guaiac acetate was 5000 μg / ml or more, and an equal weight mixture of 1,2-octanediol and guaiac acetate was 2500 μg / ml.
Further, in the same manner as in Example 1, a binary minimum growth inhibition concentration diagram was obtained. The results are shown in FIG. From the results of FIG. 3, a synergistic effect of antibacterial activity was recognized by the combination of 1,2-octanediol and guaiac acetate.
[0023]
Hereinafter, formulation examples of cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant of the present invention will be shown. In addition, a compounding quantity is weight%.

[0024]

[0025]

[0026]
【The invention's effect】
As described above in detail, the antiseptic disinfectant of the present invention has an effect of enhancing the antibacterial power inherent to 1,2-alkanediol. Therefore, it is not necessary to add a conventional antiseptic disinfectant, and high safety can be obtained.
In addition, cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant of the present invention do not need to contain conventional preservatives such as parabens, benzoic acid and salicylic acid, and the antiseptic disinfectant of the present invention is excellently enhanced. Since the antibacterial activity is high, the antiseptic disinfectant itself can be blended in a low amount, which is extremely safe.
[Brief description of the drawings]
1 is a binary minimum growth inhibitory concentration chart of Example 1. FIG.
FIG. 2 is a diagram showing an example of a method for determining an action effect caused by blending two kinds of antibacterial substances from binary minimum growth inhibitory concentrations.
3 is a binary minimum growth inhibitory concentration chart of Example 2. FIG.

Claims (6)

  An antiseptic disinfectant comprising a 1,2-alkanediol having 5 to 10 carbon atoms and isobornyl acetate and / or guaiac acetate.   The antiseptic disinfectant according to claim 1, wherein the 1,2-alkanediol is 1,2-hexanediol and / or 1,2-octanediol.   The antiseptic disinfectant according to claim 1, wherein the 1,2-alkanediol is 1,2-octanediol. A cosmetic comprising the antiseptic disinfectant according to any one of claims 1 to 3 as an antiseptic disinfectant . A pharmaceutical product comprising the antiseptic disinfectant according to any one of claims 1 to 3 as an antiseptic disinfectant . The foodstuff which mix | blended the antiseptic disinfectant in any one of Claims 1-3 as an antiseptic disinfectant.

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