JP3635579B2 - Antiseptic disinfectant and cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant - Google Patents

Description

  The present invention relates to an antiseptic fungicide and cosmetics, pharmaceuticals and foods containing the antiseptic fungicide, the purpose of which is by blending a 1,2-alkanediol having 5 to 10 carbon atoms and a specific perfume ingredient. An antiseptic fungicide that enhances the antibacterial activity inherent to 1,2-alkanediol against a wide range of bacterial species, pharmaceuticals and foods containing the antiseptic fungicide, and 1,2-alkanediol having 6 to 10 carbon atoms, An object of the present invention is to provide a cosmetic containing a preservative disinfectant characterized by containing citronellol.

  Parabens, benzoic acids, salicylic acids, and the like are used as antiseptics for cosmetics (including quasi-drugs), pharmaceuticals, and foods. However, the above-mentioned conventional antiseptic disinfectants have a drawback that the concentration range in use is easily limited because of low safety such as high skin irritation. For example, the use restriction concentration of paraben and benzoate is 1%, and the use restriction concentration of benzoic acid and salicylic acid is 0.2%. In addition, these antiseptic disinfectants are susceptible to the influence of pH, so the antiseptic disinfection effect is poor in stability, and the antibacterial and antibacterial activity may be significantly reduced by the combined use with other components such as surfactants. There was also a problem. Also, in recent years, an increasing number of people have an allergic reaction to these antiseptic disinfectants, so the direction toward safety has increased and cosmetics with no or no preservative antiseptics have been added. Demand for food, pharmaceuticals and food is increasing.

  As a technique for reducing or eliminating the antiseptic disinfectant, an antiseptic disinfectant composed of 1,2-alkanediol (see Patent Document 1) and a detergency or non-detergent cosmetic moisturizing bacteriostatic agent composed of 1,2-octanediol (See Patent Document 2) and the like. However, when a 1,2-alkanediol such as 1,2-octanediol is used alone as an antiseptic disinfectant, it is necessary to increase the blending amount when a nonionic surfactant is present. Moreover, since 1,2-alkanediol has a specific raw material odor, it has a problem of odor when blended in cosmetics and the like.

  In addition, as a technique related to an antiseptic fungicide using 1,2-alkanediol, an external composition (see Patent Document 3) formulated by combining 1,2-pentanediol and 2-phenoxyethanol, and 1,2-alkanediol An antiseptic disinfectant comprising paraben (see Patent Document 4) is disclosed. However, these are intended to improve the effect of the preservative by using a preservative and a 1,2-alkanediol such as 1,2-pentanediol in an attempt to completely eliminate the preservative itself. Not what you want.

  On the other hand, fragrances are generally blended in cosmetics and foods in order to give products rich fragrance. This perfume itself has long been known to have antibacterial properties. For example, cresyl acetate, methyl eugenol, heliotropin and ethyl salicylate, which are perfume ingredients, are anti-cariogenic agents that have antibacterial effects against mutans bacteria. It is disclosed (see Patent Document 5). However, the antibacterial effect of only the fragrance alone has a problem that the antiseptic sterilization effect of cosmetics and the like cannot be sufficiently obtained.

Japanese Patent Laid-Open No. 11-322591 JP 2001-48720 A JP-A-10-53510 JP 11-310506 A Japanese Patent Publication No. 5-67608

  In view of this situation, as a result of intensive studies by the present inventors, when 1,2-alkanediol and a specific fragrance component are used together, antibacterial activity inherently possessed by 1,2-alkanediol against a wide range of bacterial species It was found that the raw material odor of 1,2-alkanediol was also masked, and the present invention was completed.

The invention according to claim 1 relates to an antiseptic fungicide characterized by containing 1,2-alkanediol having 5 to 10 carbon atoms and citronellol.
The invention as set forth in claim 2 is characterized in that the 1,2-alkanediol is 1,2-hexanediol and / or 1,2-octanediol. It relates to the preservative disinfectant described.
The invention described in claim 3 relates to the antiseptic fungicide according to claim 1, wherein the 1,2-alkanediol is 1,2-octanediol.
The invention described in claim 4 relates to a cosmetic comprising a preservative disinfectant containing a C6-C10 1,2-alkanediol and citronellol.
The invention described in claim 5 relates to a pharmaceutical comprising the antiseptic disinfectant according to any one of claims 1 to 3.
The invention described in claim 6 relates to a food characterized by containing the antiseptic disinfectant according to any one of claims 1 to 3.

The antiseptic disinfectant according to the present invention enhances the antibacterial activity inherent to 1,2-alkanediol against a wide variety of bacterial species. Therefore, it is not necessary to add a conventional antiseptic disinfectant, and high safety can be obtained.
Further, when 1,2-hexanediol or 1,2-octanediol is used as the 1,2-alkanediol, an antiseptic sterilizer having higher antibacterial activity can be obtained.
Cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant according to the present invention do not need to contain conventional antiseptic disinfectants such as salicylic acid, benzoic acid and parabens, and the antiseptic disinfectant according to the present invention is an excellent antibacterial agent. Since it has activity, the preservative disinfectant itself can be made into a low blending amount, and it is extremely safe.

  Hereinafter, the antiseptic disinfectant according to the present invention will be described in detail. The antiseptic disinfectant according to the present invention comprises 1,2-alkanediol having 5 to 10 carbon atoms and citronellol.

  The first component of the antiseptic disinfectant according to the present invention is a 1,2-alkanediol having 5 to 10 carbon atoms represented by the following formula 1 (chemical formula 1), specifically, 1,2-pentanediol. 1,2-hexanediol, 1,2-heptanediol, 1,2-octanediol, 1,2-nonanediol, 1,2-decanediol.

（但し、式中、ｎは２〜７の整数を示す。）

(In the formula, n represents an integer of 2 to 7.)

In the present invention, one of the above-mentioned 1,5-alkanediols having 5 to 10 carbon atoms can be used alone, or two or more kinds can be mixed and used.

  1,2-alkanediol itself has excellent antibacterial activity, and has the effect of increasing the antibacterial activity of the antiseptic disinfectant according to the present invention. In particular, in the present invention, since it exhibits an excellent antibacterial action against fungi such as general bacteria, yeast and mold, among 1,2-alkanediols having 5 to 10 carbon atoms, 1,2-hexanediol, 1 1,2-octanediol is preferred, and 1,2-octanediol is more preferred.

The second component of the antiseptic disinfectant according to the present invention is citronellol.
The second component is a fragrance component, which has the effect of masking the raw material odor peculiar to 1,2-alkanediol and enhancing the antibacterial activity.
Citronellol is a colorless liquid with a sweet rose-like aroma of C ₁₀ H ₂₀ O. It exists in citronella oil, geranium oil, rose oil, etc., and is obtained by reducing citronellal.

  In the antiseptic disinfectant according to the present invention, the contents of the 1,2-alkanediol having 5 to 10 carbon atoms as the first component and the perfume component as the second component are not particularly limited. 5: 1 to 100: 1, preferably 1: 1 to 10: 1. If 1,2-alkanediol is added in an amount exceeding 100 times the blending amount of the fragrance component, an antibacterial activity enhancing effect cannot be expected, and this is not preferred. Is not preferred because it becomes too strong.

  Since the antiseptic disinfectant according to the present invention is a combination of 1,2-alkanediol as the first component and citronellol as the second component, the perfume component has a raw material odor of 1,2-alkanediol. In addition to masking, as shown in the test described later, the synergistic effect of the first component and the second component exerts an excellent antiseptic sterilization effect against all bacteria such as general bacteria, yeast, and fungi . Therefore, it is not necessary to blend or blend a preservative disinfectant that has been used in the past, such as paraben, benzoic acid, and salicylic acid, and extremely high safety can be obtained.

  The antiseptic disinfectant according to the present invention described above can be used by being blended in cosmetics, pharmaceuticals, foods and the like.

  Specifically, cosmetics for skin such as face wash, lotion, milky lotion, cream, foundation, mascara, nail enamel, lipstick, shampoo, hair treatment, hair nourishing and hair restorer, hair cream, hair lotion, hair foam, Hair cosmetics such as permanent wave agents, medicinal cosmetics (quasi-drugs) with specific uses such as stains and freckles, acne medicines, gargles, lozenges, chewing gum, candy, It can use suitably for foodstuffs, such as a drink.

  When preparing cosmetics, pharmaceuticals, or foods using the antiseptic disinfectant according to the present invention, the ingredients normally used in cosmetics, pharmaceuticals, or foods are arbitrarily blended appropriately as long as the effects of the present invention are not impaired. Can do. For example, in the case of cosmetics and pharmaceuticals (including quasi drugs), fats and oils, waxes, higher fatty acids, lower alcohols, higher alcohols, sterols, fatty acid esters, humectants, surfactants, polymer compounds, inorganic pigments, Examples include pigments, fragrances, antioxidants, ultraviolet absorbers, vitamins, astringents, whitening agents, animal and plant extracts, sequestering agents, purified water, and the like.

  In the case of food, animal and vegetable oils, polysaccharides, sweeteners, colorants, gum bases and the like can be exemplified.

  When preparing cosmetics, pharmaceuticals or foods, the compounding amount of the antiseptic disinfectant according to the present invention is not particularly limited, but is 0.01 to 20% by weight, preferably 0.05 to 5% by weight in the composition. . Even if it exceeds 20% by weight, no further effect can be expected. Moreover, when it is less than 0.01% by weight, the antibacterial effect is inferior, which is not preferable.

[Comparative Example 1]
As test bacteria, Candida albicans IFO1594 (oral candidiasis), Staphylococcus aureus IFO13276 (Staphylococcus aureus), and Escherichia coli IFO3972 (E. coli) were used.

(Preparation of bacterial solution for inoculation)
In the case of Staphylococcus aureus and Escherichia coli, the bacterial solution for inoculation was cultured on an agar medium at 35 ° C., transplanted to a bouillon medium, and cultured at 35 ° C. A solution obtained by diluting the obtained culture solution to about 10 ⁸ cells / ml with a bouillon medium was used as a bacterial solution for inoculation.
In the case of yeast (oral candidiasis), the same culture at 30 ° C. and diluted to about 10 ⁷ cells / ml were used as the inoculum.

(Preparation of dilution series of test substance)
20 w / w% ethyl cellosolve as a diluent solvent, 5, 4, 3, 2.5, 2.25, 2, 1.75, 1.5, 1.25, 1 w / v% 1,2- An octanediol solution was prepared.
Moreover, about the equiweight mixture of isobornyl acetate and 1, 2- octanediol, and isobornyl acetate, the liquid of 20 w / v% was diluted twice and the dilution series was prepared.

(Measurement of minimum inhibitory concentration (MIC))
For each 1 ml of dilution series containing the test substance, 9 ml of each agar medium was placed in a petri dish, and the inoculum for the inoculum was streaked to a length of about 1 cm.
For staphylococcus aureus and Escherichia coli, the culture was performed at 35 ° C., and the presence or absence of the growth of the bacteria after 2 days was determined. Moreover, about yeast, it culture | cultivated at 25 degreeC and the presence or absence of the growth of a microbe after 3 days was determined. At this time, the minimum concentration at which no growth was observed was determined as MIC.
The antibacterial activity can be evaluated by the minimum inhibitory concentration (MIC). There is no effect on microorganisms when the concentration of the test substance is low, but growth inhibition occurs when the concentration is increased. This degree of growth proceeds depending on the concentration, and finally the growth stops. The concentration at that time is expressed as MIC. Therefore, when the concentration is higher than MIC, the microorganisms are killed.

(Evaluation of dual minimum growth inhibitory concentration)
Each MIC of the resulting 1,2-octanediol, isobornyl acetate and an equal weight mixture of 1,2-octanediol and isobornyl acetate was plotted against the blending amount of 1,2-octanediol and isobornyl acetate, A binary minimum inhibitory concentration diagram was determined.
In addition, the effect at the time of mix | blending two types of substances which have antimicrobial property can be determined with a binary minimum growth inhibitory density | concentration. Specifically, when two types of substances having antibacterial properties are blended, the resulting action is roughly classified into synergistic action, additive action, and antagonism. The synergistic action is an action in which two drugs act synergistically to further enhance the inherent antibacterial power. The additive action is an action in which the antibacterial powers of the respective drugs are combined. Antagonism is the action when one drug counteracts the antibacterial activity of another drug. The method based on the binary minimum growth inhibitory concentration is a method for determining from the graph by measuring the MIC for each of the A substance and B substance while changing their ratios, for example, as shown in FIG. According to this, when MIC (point A) in only substance A and MIC (point B) only in substance B are connected, the MIC when both substances are used is on the inner side of this line (point C) is a synergistic effect in which antibacterial activity is enhanced by the combined use, when it is on the line (point D), when it is an additive action, when it is outside the line (point E), one or both It can be determined that the antibacterial activity is an antagonism that counteracts the antibacterial activity and decreases the antimicrobial activity.
FIG. 3 shows a binary minimum growth inhibitory concentration diagram for isobornyl acetate.

(Evaluation of antibacterial effect)
From the binary minimum inhibitory concentration diagram shown in FIG. 3, the antibacterial effect when 1,2-octanediol and isobornyl acetate were mixed was determined according to the following evaluation criteria. The results are listed in Table 1.

<Evaluation criteria>
○: Synergistic action is recognized in antibacterial effect.
Δ: Additive action is observed in antibacterial effect.
X: Antagonism is recognized in antibacterial effect.

[Comparative Example 2]
As the fragrance component, guaiac acetate was operated in the same manner as in Comparative Example 1 to obtain the binary minimum growth inhibition concentration diagram of FIG. The obtained results were evaluated for antibacterial effect according to the above evaluation criteria. The results are shown in Table 1.

[Example 1]
Using citronellol as a fragrance component, the same operation as in Comparative Example 1 was performed, and the binary minimum growth inhibitory concentration diagram of FIG. 2 was obtained. The obtained results were evaluated for antibacterial effect according to the above evaluation criteria. The results are shown in Table 1.

  From the results of Table 1, it can be seen that when 1,2-alkanediol is used in combination with citronellol which is a specific perfume component, the antibacterial activity inherent to 1,2-alkanediol is enhanced against a wide range of bacterial species.

  On the other hand, when 1,2-alkanediol and other perfume ingredients such as isobornyl acetate or guaiac acetate are used in combination, it is understood that the antibacterial activity enhancing action cannot be obtained for a wide range of bacterial species.

Hereinafter, the example of a foodstuff which mix | blended the antiseptic disinfectant which concerns on this invention is shown.
<Prescription Example 1: Beverage>
Glucose liquid sugar 33.0
Grapefruit juice 64.0
1,2-pentanediol 0.5
Citronellol 0.01
Fragrance 0.5
Acidulant appropriate amount
Total 100.0% by weight

  The antiseptic disinfectant according to the present invention enhances the antibacterial activity inherent to 1,2-alkanediol against a wide range of bacterial species and also has high safety, so that it can be used in cosmetics, pharmaceuticals, foods, etc. It can be suitably used as an antiseptic disinfectant.

It is a figure which shows an example of the method of judging the effect produced by the case where two types of substances which have antimicrobial property are mix | blended from binary minimum growth inhibitory density | concentration. It is a binary minimum growth inhibitory concentration diagram regarding citronellol. FIG. 2 is a binary minimum inhibitory concentration diagram for isobornyl acetate. It is a binary minimum growth inhibitory concentration diagram regarding guaiac acetate.

Claims (6)

An antiseptic disinfectant comprising a 1,2-alkanediol having 5 to 10 carbon atoms and citronellol. The antiseptic disinfectant according to claim 1, wherein the 1,2-alkanediol is 1,2-hexanediol and / or 1,2-octanediol. The antiseptic disinfectant according to claim 1, wherein the 1,2-alkanediol is 1,2-octanediol. A cosmetic comprising a 1,2-alkanediol having 6 to 10 carbon atoms and a preservative disinfectant containing citronellol. A pharmaceutical product comprising the antiseptic disinfectant according to any one of claims 1 to 3. A food comprising the antiseptic disinfectant according to any one of claims 1 to 3.

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