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ES2170622B1 - Clones y vectores infectivos derivados de coronavirus y sus aplicaciones. - Google Patents

Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.

Info

Publication number
ES2170622B1
ES2170622B1 ES009902673A ES9902673A ES2170622B1 ES 2170622 B1 ES2170622 B1 ES 2170622B1 ES 009902673 A ES009902673 A ES 009902673A ES 9902673 A ES9902673 A ES 9902673A ES 2170622 B1 ES2170622 B1 ES 2170622B1
Authority
ES
Spain
Prior art keywords
infective
coronavirus
clones
applications
vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES009902673A
Other languages
English (en)
Other versions
ES2170622A1 (es
Inventor
Luis Enjuanes Sanchez
Fernando Almazan Toral
J.Manuel Gonzalez Martinez
Ander Izeta Permisan
Zoltan Penzes
Sara Alonso Villanueva
Carlos Sanchez Sanchez
Isabel Sola Gurpegui
J. Manuel Sanchez Morgado
Enrique Calvo Alcocer
Francisco J. Ortego Alonso
David Escors Murugarren
Patricio Barrado Guerrero
Cristina Riquelme Gabriel
Juan Plana Duran
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8310818&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2170622(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES009902673A priority Critical patent/ES2170622B1/es
Priority to PCT/EP2000/012063 priority patent/WO2001039797A2/en
Priority to EP04007406A priority patent/EP1437400A3/en
Priority to KR1020027007118A priority patent/KR100755814B1/ko
Priority to EP00990628A priority patent/EP1234024B2/en
Priority to SI200030394T priority patent/SI1234024T2/sl
Priority to PT00990628T priority patent/PT1234024E/pt
Priority to SK761-2002A priority patent/SK7612002A3/sk
Priority to AT00990628T priority patent/ATE267246T1/de
Priority to TR2004/01186T priority patent/TR200401186T4/xx
Priority to CNA2004100789786A priority patent/CN1616670A/zh
Priority to DK00990628.0T priority patent/DK1234024T4/da
Priority to CNA2006100733455A priority patent/CN1872994A/zh
Priority to DE60010921T priority patent/DE60010921T3/de
Priority to IL14985900A priority patent/IL149859A0/xx
Priority to HU0203305A priority patent/HU228205B1/hu
Priority to JP2001541529A priority patent/JP4637432B2/ja
Priority to MXPA02005525A priority patent/MXPA02005525A/es
Priority to NZ518990A priority patent/NZ518990A/en
Priority to US10/148,669 priority patent/US7445928B2/en
Priority to CNB008165904A priority patent/CN100402643C/zh
Priority to BR0016145-4A priority patent/BR0016145A/pt
Priority to CZ20021882A priority patent/CZ302785B6/cs
Priority to YUP-375/02A priority patent/RS50372B/sr
Priority to PL356390A priority patent/PL206816B1/pl
Priority to AU30051/01A priority patent/AU785081B2/en
Priority to CA002393325A priority patent/CA2393325A1/en
Priority to ES00990628T priority patent/ES2218276T5/es
Priority to ARP000106406A priority patent/AR027893A1/es
Priority to ZA200203560A priority patent/ZA200203560B/xx
Priority to NO20022419A priority patent/NO331812B1/no
Priority to IL149859A priority patent/IL149859A/en
Publication of ES2170622A1 publication Critical patent/ES2170622A1/es
Priority to US10/238,786 priority patent/US7368557B2/en
Publication of ES2170622B1 publication Critical patent/ES2170622B1/es
Application granted granted Critical
Priority to AU2006203327A priority patent/AU2006203327B2/en
Priority to US12/285,213 priority patent/US20100167352A1/en
Priority to US12/938,771 priority patent/US20110142879A1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/215—Coronaviridae, e.g. avian infectious bronchitis virus
    • A61K39/225—Porcine transmissible gastroenteritis virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5256—Virus expressing foreign proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
    • A61K2039/541—Mucosal route
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
    • C12N2770/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
    • C12N2770/20041—Use of virus, viral particle or viral elements as a vector
    • C12N2770/20043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
    • C12N2770/20061—Methods of inactivation or attenuation
    • C12N2770/20062—Methods of inactivation or attenuation by genetic engineering
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Virology (AREA)
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  • Veterinary Medicine (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Communicable Diseases (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Pulmonology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oncology (AREA)
  • Pain & Pain Management (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Clones y vectores infectivos derivados de coronavirus y sus aplicaciones. El clon infectivo derivado de un coronavirus comprende un cDNA que codifica el gRMA de un coronavirus clonado bajo una secuencia promotora de la transcripción. El vector viral recombinante comprende un clon infectivo modificado para contener un ácido nucleico heterólogo insertado en dicho clon infectivo bajo condiciones que permiten la expresión de dicho ácido nucleico heterólogo. Los clones y vectores infectivos son útiles tanto en investigación básica como aplicada, en el desarrollo de sistemas de expresión eficientes de productos de interés (proteínas, enzimas, anticuerpos, etc.), vectores vacunales y terapia génica.
ES009902673A 1999-12-03 1999-12-03 Clones y vectores infectivos derivados de coronavirus y sus aplicaciones. Expired - Fee Related ES2170622B1 (es)

Priority Applications (36)

Application Number Priority Date Filing Date Title
ES009902673A ES2170622B1 (es) 1999-12-03 1999-12-03 Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.
BR0016145-4A BR0016145A (pt) 1999-12-03 2000-11-30 Clones infecciosos
IL14985900A IL149859A0 (en) 1999-12-03 2000-11-30 Infectious clones
KR1020027007118A KR100755814B1 (ko) 1999-12-03 2000-11-30 감염성 클론
EP00990628A EP1234024B2 (en) 1999-12-03 2000-11-30 Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant rna-virus
SI200030394T SI1234024T2 (sl) 1999-12-03 2000-11-30 Umetni kromosomi, ki vsebujejo sekvence nukleinskih kislin, sposobne usmerjanja tvorbe rekombinantnega rna virusa
PT00990628T PT1234024E (pt) 1999-12-03 2000-11-30 Construcoes de cromossomas artificiais contendo sequencias de acido nucleico capazes de dirigir a formacao de um virus de rna recombinante
US10/148,669 US7445928B2 (en) 1999-12-03 2000-11-30 Bacterial artificial chromosome construct encoding recombinant coronavirus
AT00990628T ATE267246T1 (de) 1999-12-03 2000-11-30 Künstliche chromosomen, die die genetische information für die bildung von rekombinantem rna-virus enthalten
TR2004/01186T TR200401186T4 (tr) 1999-12-03 2000-11-30 Rekombinant bir RNA-virüs oluşumunu yönlendirebilen nükleik asit sekansları içerikli suni kromozom yapıları.
CNA2004100789786A CN1616670A (zh) 1999-12-03 2000-11-30 感染性克隆
DK00990628.0T DK1234024T4 (da) 1999-12-03 2000-11-30 Kunstige chromosomkonstruktioner, der indeholder nucleinsyresekvenser, som er i stand til at dirigere dannelsen af et rekombinant RNA-virus
CNA2006100733455A CN1872994A (zh) 1999-12-03 2000-11-30 感染性克隆
DE60010921T DE60010921T3 (de) 1999-12-03 2000-11-30 Künstliche chromosomen, die die genetische information für die bildung von rekombinantem rna-virus enthalten
EP04007406A EP1437400A3 (en) 1999-12-03 2000-11-30 Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant rna-virus
HU0203305A HU228205B1 (en) 1999-12-03 2000-11-30 Infectious clones
JP2001541529A JP4637432B2 (ja) 1999-12-03 2000-11-30 感染性クローン
MXPA02005525A MXPA02005525A (es) 1999-12-03 2000-11-30 Clones infecciosos.
PCT/EP2000/012063 WO2001039797A2 (en) 1999-12-03 2000-11-30 Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant rna-virus
SK761-2002A SK7612002A3 (en) 1999-12-03 2000-11-30 Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant rna-virus
CNB008165904A CN100402643C (zh) 1999-12-03 2000-11-30 感染性克隆
NZ518990A NZ518990A (en) 1999-12-03 2000-11-30 Use of bacterial artificial chromosomes (BACs) to prepare heterologous DNA expression vectors to formulate vaccines
CZ20021882A CZ302785B6 (cs) 1999-12-03 2000-11-30 Zpusob prípravy DNA odvozené od koronaviru, príslušné infekcní klony, vektory a vakcíny
YUP-375/02A RS50372B (sr) 1999-12-03 2000-11-30 Infektivni klonovi
PL356390A PL206816B1 (pl) 1999-12-03 2000-11-30 Sposób otrzymywania DNA, klon zakaźny, E.coli, rekombinowany wektor wirusowy i szczepionka do ochrony zwierzęcia
AU30051/01A AU785081B2 (en) 1999-12-03 2000-11-30 Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant RNA-virus
CA002393325A CA2393325A1 (en) 1999-12-03 2000-11-30 Infectious clones
ES00990628T ES2218276T5 (es) 1999-12-03 2000-11-30 Constructos de cromosomas artificiales que contienen secuencias de acidos nucleicos capaces de dirigir la formacion de un virus recombinante de arn.
ARP000106406A AR027893A1 (es) 1999-12-03 2000-12-04 Clones infecciosos
ZA200203560A ZA200203560B (en) 1999-12-03 2002-05-06 Infectious clones.
NO20022419A NO331812B1 (no) 1999-12-03 2002-05-22 Fremgangsmate for a fremstille DNA avledet fra coronavirus samt infeksiose kloner, rekombinant viral vektor samt vaksine
IL149859A IL149859A (en) 1999-12-03 2002-05-27 Tribes of pollutants
US10/238,786 US7368557B2 (en) 1999-12-03 2002-09-11 Polynucleotides encoding porcine transmissible gastroenteritis virus
AU2006203327A AU2006203327B2 (en) 1999-12-03 2006-08-03 Infectious clones
US12/285,213 US20100167352A1 (en) 1999-12-03 2008-09-30 Infectious clones
US12/938,771 US20110142879A1 (en) 1999-12-03 2010-11-03 Infectious clones

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009902673A ES2170622B1 (es) 1999-12-03 1999-12-03 Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.

Publications (2)

Publication Number Publication Date
ES2170622A1 ES2170622A1 (es) 2002-08-01
ES2170622B1 true ES2170622B1 (es) 2004-05-16

Family

ID=8310818

Family Applications (2)

Application Number Title Priority Date Filing Date
ES009902673A Expired - Fee Related ES2170622B1 (es) 1999-12-03 1999-12-03 Clones y vectores infectivos derivados de coronavirus y sus aplicaciones.
ES00990628T Expired - Lifetime ES2218276T5 (es) 1999-12-03 2000-11-30 Constructos de cromosomas artificiales que contienen secuencias de acidos nucleicos capaces de dirigir la formacion de un virus recombinante de arn.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES00990628T Expired - Lifetime ES2218276T5 (es) 1999-12-03 2000-11-30 Constructos de cromosomas artificiales que contienen secuencias de acidos nucleicos capaces de dirigir la formacion de un virus recombinante de arn.

Country Status (27)

Country Link
US (4) US7445928B2 (es)
EP (2) EP1437400A3 (es)
JP (1) JP4637432B2 (es)
KR (1) KR100755814B1 (es)
CN (3) CN100402643C (es)
AR (1) AR027893A1 (es)
AT (1) ATE267246T1 (es)
AU (2) AU785081B2 (es)
BR (1) BR0016145A (es)
CA (1) CA2393325A1 (es)
CZ (1) CZ302785B6 (es)
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