DE2802397A1 - Animal feedstuffs giving high feed utilisation and wt. gain - contg. Nitrovin, Carbadox or Olaquindox and proteolytic enzyme - Google Patents

Abstract

Animal feedstuff mixts., based on carbohydrates, proteins and fats, contain (A) 5-350 ppm of a growth accelerator selected from nitrovin, carbadox and olaquindox and (B) proteolytic enzymes in amts. corresp. to an enzymatic activity of 0.05-2.5 mUT/g. (B) are pref. acid proteases active at pH 2.5-6.5. The combination of (A) and (B) gives esp. high feed utilisation and wt. gain, esp. in (suckling) pigs and also in poultry.

Description

"Animal feed mix"

The subject of the main patent (patent application P 27 55 126.4) is a Animal feed mixture based on carbohydrates, protein, fats and possibly usual additives, characterized by a content of s - 350 ppm of the growth promoter Nitrovin and such an amount of proteolytic enzymes that an enzymatic one Activity of 0.05-2.5 mTU / g is present.

It was invented that high quality animal feed mixtures could then be used in corresponding growth-improving effectiveness, if you get the growth sponsor of the main patent through one of the growth promoters Carbadox or Olaqundox replaced.

The invention relates to an animal feed mixture on the basis of carbohydrates, protein, fats and, if necessary, the usual additives according to patent (patent application P 27 55 126.4 (D 5692)), characterized by a Content of 5 - 350 ppm of one of the growth promoters Carbadox or Olaquinox and one such amount of proteolytic enzymes that an enzymatic activity of 0.05 - 2.5 mTU / g is present.

The growth promoter Carbadox is produced by chemical synthesis. Its noelenklarturce right name is. Methyl 3- (2-quinoxalinyl methylene). The molecular formula is: C11H10N4O4, the molecular weight is 262.23.

The growth promoter olaquinox is also made through chemical synthesis won.

Its nomenclature-appropriate designation is: 2- [N- (2-hydroxyethyl) carbamoyl] -3-methyl-quinoxaline-1,4-dioxide. Its molecular formula is: C12H13N3O4, the molecular g @ ent @@ @@ 203, @.

Suitable proteolytic enzymes are mentioned before ailc: r. by breeding obtained from microorganisms and separating from the nutrient solutions.

The procedures for this are known. For example, be proteolytic Enzymes from i3acillus licheniformis, Bacillus natta, Bacillus subtilis, etc. are used. Acid proteases, e.g. B. from Aspergillus niger or from Rhizopus rhizopodiformis according to patent application P 25 28 490.6. Such proteases have a particularly broad spectrum of activity in the weakly acidic range between pH 2.5 - 6.5.

Commercially available feed mixes are for special needs of the individual animal species put together as optimally as possible. Form the basis Carbohydrates from all cereal components, nais or the like ... As a protein carrier They are primarily soy meal, fish meal, carcass meal, bran and the like. Missing Amino acids, e.g. B.

Methionine or lysine can also be added.

Fats come in the form of vegetable or animal fats or Waste fats included. Salts such as dicalcium phosphate, Calcium carbonate, table salt added. If necessary, the feed mixture is through Addition of trace elements, vitamins, fiber, etc. rounded off. Aucii fermentative manufactured substances such as single-cell proteins from petroleum fractions or alcohols, various yeasts, algae protein or other, possibly also obtained from waste materials Substances can e.g. T. be part of the feed recipe to a considerable extent.

The animal feed mixtures according to the invention contain in addition to the usual For a specific species of animal, an additional 5 - 350 ppm, preferably 20-50 ppm of one of the named growth promoters, depending on age of the animal and the type of feed mixture and proteolytic enzymes in one such an amount that the enzymatic activity 0.05-2.5 mTU / g, preferably 0.2 - 0.5 mTU / g amount The additive concentrations mentioned refer to the content in total lining. Concentrates are the same for concentrates or supplementary feeds correspondingly higher. In particular, a combination of one of the named growth promoters and an acidic fungal protease or

Protease mixture used. The growth achieved in this way or the Improvement in feed conversion is also several percent in comparison to corresponding control mixtures containing only Cabadox or Olaquindox.

The claimed combination of carbadox or olaquindox and enzymes has proven itself especially in feed for piglet rearing and pig fattening. she can however, it can also be used in feed mixes for other animal species in which the growth conveyor itself is used to advantage.

The growth promoter is expediently in the form of a premix Feed added, e.g. B. bound to soybean meal. When using a throat-shaped The enzyme component is also applied as a premix to feed compositions. Any feed component, e.g. B. also soy meal or an inert carrier. When using steam-pelleted feed, the addition must be made the enzymes take place in a suitably stabilized form in order to be deactivated Prevent moisture and heat during pelleting.

Such a method is e.g. ß. Subject of the patent (patent application P 26 02 260.6).

The proteolytic activity is used to determine the enzyme unit (TU). the protease is determined according to the well-known principle of anion: a suitably diluted one close enzyme solution is at 40 ° C for 20 minutes with an equal volume of a 1.2 % casein solution incubated, this being 0.6% lactic acid, 6 mol urea and Contains 0.1 mol of citric or acetic acid. The pH of the casein solution is determined by Addition of 2 N sodium hydroxide solution adjusted to 4.5. After the incubation is done in volume ratio 1: 1 mixed with 0.4 H trichloroacetic acid, the precipitate formed of The undigested casein is filtered off and the protein fragments resulting from the breakdown are in the filtrate determined by any protein determination method. Suitable for this is z. B. that by Layne in the Method of Enzymology 3 (1957) pages 448 gg. Described Procedure.

A blank value must be prepared for each measurement sample, with which first Trichloroacetic acid and then casein solution is added. This blank value gives besides the reagent blank value shows the proportion of low molecular weight peptides that has already been is present in the enzyme solution prior to digestion.

The difference between the home and blank values is given at the Method then compared with the absorbance that a certain amount of tyrosine at this provision delivers.

This amount of tyrosine is then a measure of the proteolytic activity of the present enzyme: an enzyme unit (TU) is the amount of enzyme that is made up of a casein solution releases cleavage products in one minute which have the same extinction like a 1 M tyrosine solution. Usually it is specified in mTU = 10 -3 TU.

Example 1: 216 piglets in the weight section of # 6.5 kg up to # 24.8 kg fed an identically composed rearing feed, but that a) without the addition of carbadox or enzyme, b) an addition of 50 ppm carbadox or

c) an addition of 50 ppm carbadox and 0.45 mTU / g feed acid Proteases from Rhizopus rhiopodiformis and Aspergillus.

The animals were placed in flat cages of 7 or 3 animals. the Feeding was ad libidum. To get used to it, all groups were given an identical one compound starter feed administered. Then the animals received the actual test mixtures (see above) The growth process was determined by regular weighing and determines the feed conversion rate. "Feed conversion" is the ratio Lutter consumption: Increase understood.

Feed composition (%): protein feed consisting of: 18% 62.5% Soy meal (44% crude protein), 25% fish meal, 12.5% minerals and vitamin premix Oats 14% wheat bran 10% barley 28% wheat 15% corn 15% 100% Result: a) b) c) without addition with carbadox with carbadox + enzyme daily.

Increase 461 g 526 g 543 g Feed conversion 2.11 1.93 1.91 Example 2: A total of 43 piglets were kept on slatted floors without straw from about 30 years old kg initial weight in two mast sections up to the final weight of approx. 100 kg in each case identical to the high-volume feed mixture fed to those in the fattening section I (30-60 kg) a) without the addition of carbadox or enzyme, b) an addition of 50 ppm carbadox or

c) an addition of 50 ppm carbadox and 0.6 mTU / g feed acidic proteases from Rdizopus rhiopodiformis.

In the fattening section II (60-100 kg) were in the appropriate groups the following amounts of additives are used: a) without the addition of carbadox or enzyme b) addition of 25 ppm carbadox c) addition of 25 ppm carbadox and 0.3 mTU / g feed acidic proteases from Rhizopus rhizopodiformis.

The course of growth and feed conversion were determined via regular weighings certainly. Under feed conversion the ratio feed consumption / increase Understood. The animals were fed rationed (twice a day) in accordance with the DLG recommendations.

Feed composition (%) starting fattening finishing fattening 30 - 60 kg 60 - 100 kg barley 20 22 maize 20 22 wheat 15 22 oats 15 wheat bran 9 21.5 fish meal 7 3 Soy meal 12 8 Mineral feed 2 1.5 Result: 30 - 100 kg total fattening a) b) c) without addition with carbadox with carbado + enzyme daily.

Increase 674 g 722 g 753 Feed conversion 3.31 3.18 3.14 Example 3: I1 weight segment 11-25 kg was given an identically composed piglet for every 10 piglets Rearing feed was fed, but a) without the addition of olaquindox or enzyme b) an addition of 30 ppm olaquindox or

c) an addition of 30 ppm olaquindox and 0.5 mTU / g feed acid Exhibited proteases from Aspergillus niger.

The experiment was carried out under the conditions of floor housing with straw litter. Feeding took place ad libidum. Analogous to the methodology mentioned in Examples 1 and 2 the daily weight gain and the putter utilization were determined.

Feed composition (%): barley 15 oats 15 corn 20 wheat 10 wheat bran 17 green meal 2 soy meal 12 fish meal 6 mineral feed 3 100 result: a) b) c) without addition with olaquindox with olaquindox + enzyme daily.

Gain: 492 g 530 g 557 g Feed conversion: 2.05 2.04 2.00 Example 4: Analogously to the conditions in example 2, ilil weight sections 30-100 kg each 8 pigs fed identical feed mixes, but the a) without the addition of olaquindox or enzyme, b) an addition of 100 ppm olaquindox respectively.

c) an addition of 80 ppm olaquinox around 0.6 mTU / g feed acidic proteases from Aspergillus niger and Rhizopus rhizopodiformis.

The concentrations of the additives mentioned remained the same over the entire period Fattening duration unchanged. Feeding methodology and feeding composition corresponded the example 2.

Result: a) b) c) without addition with olaquindox with olaquindox + enzyme Daily

Increase 640 5 671 g 696 g Feed conversion 3.27 3.19 3.13

Claims (2)

"Animal feed mixture" (addition to the patent (patent application P 27 55 126.4 (D 5692)) P a t e n t a n s p r ü c n e 1. Animal feed mixture based on of carbohydrates, protein, fats and, if necessary, customary additives according to Patent (patent application P 27 55 126.4 (D 4692), characterized by a content at 5 - 350 ppm of one of the growth promoters Carbadox or Olaquindox and one of these Amount of proteolytic enzymes that have an enzymatic activity of 0.05 - 2.5 mTU / g is present. 2. animal feed mixture according to claim 1, characterized in that the proteolytic enzymes acidic proteases with a spectrum of activity between pH 2.5 - 6.5 are.