RU2006132051A - Окисляемые соединения в качестве внутреннего стандарта для биосенсоров и способ их применения - Google Patents
Окисляемые соединения в качестве внутреннего стандарта для биосенсоров и способ их применения Download PDFInfo
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- RU2006132051A RU2006132051A RU2006132051/13A RU2006132051A RU2006132051A RU 2006132051 A RU2006132051 A RU 2006132051A RU 2006132051/13 A RU2006132051/13 A RU 2006132051/13A RU 2006132051 A RU2006132051 A RU 2006132051A RU 2006132051 A RU2006132051 A RU 2006132051A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3273—Devices therefor, e.g. test element readers, circuitry
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/004—Enzyme electrodes mediator-assisted
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
- C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
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- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
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- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (20)
1. Биосенсор для определения концентрации анализируемого вещества в пробе, содержащий:
смесь для электрохимической реакции с анализируемым веществом, где указанная смесь включает
фермент,
медиатор и
окисляемое вещество в качестве внутреннего стандарта.
2. Биосенсор по п.1, в котором указанный внутренний стандарт определяют как восстановленную форму обратимой окислительно-восстановительной пары, которая имеет окислительно-восстановительный потенциал, равный или выше потенциала указанного медиатора.
3. Биосенсор по п.1, в котором указанный медиатор представляет собой 3-фенилимино-3Н-фенотиазин.
4. Биосенсор по п.3, в котором указанный внутренний стандарт представляет собой ферроцианид.
5. Биосенсор по п.4, в котором указанный ферроцианид, являющийся указанным внутренним стандартом, и указанный медиатор окисляются при наложении первого потенциала, а при наложении второго потенциала окисляется только указанный медиатор; причем указанный второй потенциал ниже, чем указанный первый потенциал.
6. Биосенсор по п.5, в котором указанный первый потенциал равен примерно 400 мВ и указанный второй потенциал равен примерно 100 мВ.
7. Биосенсор по п.1, в котором указанный медиатор представляет собой феррицианид.
8. Биосенсор по п.7, в котором указанный внутренний стандарт представляет собой ферроцианид.
9. Биосенсор по п.1, в котором указанный медиатор представляет собой гексааминрутений.
10. Биосенсор по п.9, в котором указанный внутренний стандарт представляет собой ферроцианид.
11. Биосенсор по п.10, в котором указанный фермент представляет собой глюкозоксидазу.
12. Способ применения биосенсора, содержащего смесь фермента, медиатора и окисляемого соединения в качестве внутреннего стандарта, где способ включает стадии:
наложение первого потенциала в начальный период;
представление периода запаздывания;
наложение второго потенциала в заключительном периоде, следующем за периодом запаздывания, причем указанный первый потенциал и указанный второй потенциал выбирают таким образом, чтобы окислялся только указанный медиатор или оба соединения - указанный медиатор и указанный внутренний стандарт.
13. Способ по п.12, в котором стадия наложения первого потенциала в первом периоде включает стадию наложения выбранного высокого первого потенциала в первом периоде для окисления указанного медиатора и указанного внутреннего стандарта.
14. Способ по п.12, в котором стадия наложения первого потенциала в первом периоде включает стадию наложения выбранного низкого первого потенциала в первом периоде для окисления только указанного медиатора.
15. Способ по п.12, в котором стадия наложения второго потенциала в заключительном периоде, следующем за указанным периодом запаздывания, включает стадию наложения выбранного второго потенциала для окисления указанного медиатора и указанного внутреннего стандарта.
16. Способ по п.12, в котором стадия наложения второго потенциала в заключительном периоде, следующем за указанным периодом запаздывания, включает стадию наложения выбранного второго потенциала для окисления только указанного медиатора.
17. Способ по п.12, в котором стадии наложения указанного первого потенциала и наложения указанного второго потенциала включают стадии наложения выбранного второго потенциала в интервале от 100 до 400 мВ.
18. Способ по п.12, в котором стадии наложения указанного первого потенциала и наложения указанного второго потенциала включает стадии наложения выбранного первого потенциала в первом периоде для окисления как указанного медиатора, так и указанного внутреннего стандарта, и наложения выбранного второго потенциала для окисления только указанного медиатора.
19. Способ по п.12, в котором биосенсор содержит медиатор, представляющий собой одно из соединений - 3-фенилимино-3Н-фенотиазин и гексааминрутений, и в котором внутренний стандарт представляет собой ферроцианид, и в котором стадии наложения указанного первого потенциала и наложения указанного второго потенциала включают стадии наложения выбранного первого и второго потенциалов для окисления только указанного медиатора.
20. Способ по п.12, в котором стадии наложения указанного первого потенциала и наложения указанного второго потенциала включают стадии наложения выбранного первого потенциала и второго потенциала для окисления как указанного медиатора, так и указанного внутреннего стандарта, в котором указанный внутренний стандарт эффективно фиксирует отсекаемый отрезок на калибровочной кривой в узком интервале и указанный внутренний стандарт эффективно сохраняет наклон калибровочной кривой биосенсора.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US54236204P | 2004-02-06 | 2004-02-06 | |
US60/542,362 | 2004-02-06 |
Publications (1)
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RU2006132051A true RU2006132051A (ru) | 2008-03-20 |
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RU2006132051/13A RU2006132051A (ru) | 2004-02-06 | 2005-02-04 | Окисляемые соединения в качестве внутреннего стандарта для биосенсоров и способ их применения |
Country Status (13)
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US (3) | US8696880B2 (ru) |
EP (1) | EP1713926B1 (ru) |
JP (1) | JP2007523326A (ru) |
KR (1) | KR20060131836A (ru) |
CN (1) | CN1914331A (ru) |
AU (1) | AU2005212396A1 (ru) |
BR (1) | BRPI0507376A (ru) |
CA (1) | CA2553632A1 (ru) |
CR (1) | CR8597A (ru) |
MA (1) | MA28350A1 (ru) |
NO (1) | NO20063939L (ru) |
RU (1) | RU2006132051A (ru) |
WO (1) | WO2005078118A1 (ru) |
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2005
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- 2005-02-04 WO PCT/US2005/003622 patent/WO2005078118A1/en active Application Filing
- 2005-02-04 RU RU2006132051/13A patent/RU2006132051A/ru not_active Application Discontinuation
- 2005-02-04 AU AU2005212396A patent/AU2005212396A1/en not_active Abandoned
- 2005-02-04 EP EP05712898A patent/EP1713926B1/en not_active Expired - Lifetime
- 2005-02-04 CA CA002553632A patent/CA2553632A1/en not_active Abandoned
- 2005-02-04 CN CNA2005800038597A patent/CN1914331A/zh active Pending
- 2005-02-04 BR BRPI0507376-6A patent/BRPI0507376A/pt not_active IP Right Cessation
- 2005-02-04 JP JP2006552278A patent/JP2007523326A/ja not_active Withdrawn
- 2005-02-04 KR KR1020067015845A patent/KR20060131836A/ko not_active Application Discontinuation
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2006
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- 2006-09-04 NO NO20063939A patent/NO20063939L/no not_active Application Discontinuation
- 2006-09-06 CR CR8597A patent/CR8597A/es not_active Application Discontinuation
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US20070045126A1 (en) | 2007-03-01 |
BRPI0507376A (pt) | 2007-07-10 |
NO20063939L (no) | 2006-11-03 |
US10067082B2 (en) | 2018-09-04 |
CN1914331A (zh) | 2007-02-14 |
WO2005078118A1 (en) | 2005-08-25 |
MA28350A1 (fr) | 2006-12-01 |
AU2005212396A1 (en) | 2005-08-25 |
KR20060131836A (ko) | 2006-12-20 |
CR8597A (es) | 2007-02-07 |
EP1713926A1 (en) | 2006-10-25 |
US8696880B2 (en) | 2014-04-15 |
US9410917B2 (en) | 2016-08-09 |
US20160313272A1 (en) | 2016-10-27 |
EP1713926B1 (en) | 2012-08-01 |
US20140174951A1 (en) | 2014-06-26 |
JP2007523326A (ja) | 2007-08-16 |
CA2553632A1 (en) | 2005-08-25 |
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