KR102635704B1 - A COMPOSITION THAT DOES NOT IRRITATE THE SKIN AND HAS EXCELLENT SKIN MOISTURIZING PROPERTIES, WHICH CONTAINS Hemerocallis fulva L EXTRACT AS AN ACTIVE INGREDIENT - Google Patents

Abstract

The present invention relates to a skin moisturizing composition containing daylily extract as an active ingredient, which does not cause skin irritation and has an excellent skin moisturizing effect. It is provided as a cosmetic composition, and does not cause skin irritation when applied to the skin, preventing skin troubles. It does not cause skin irritation, can be used even in cases of environmental skin diseases as it contains ingredients derived from natural substances, and can have an excellent skin moisturizing effect.
Additionally, it can be provided as a food composition and have a skin moisturizing effect upon ingestion.

Description

A composition containing daylily extract as an active ingredient that does not irritate the skin and is excellent in moisturizing the skin

The present invention relates to a composition containing daylily extract as an active ingredient, which does not cause skin irritation and has excellent skin moisturizing effect.

From the outside, the skin is largely divided into three layers: epidermis, dermis, and subcutaneous fat tissue, and performs an important function of protecting the human body from physical and chemical stimuli from the external environment.

On the other hand, the skin is composed of cells, and the cells are protected by cell membranes, so when the cell membrane is damaged, the cells suffer structural and functional damage, leading to rapid skin damage and aging. Human skin As we age, we experience changes due to various internal and external factors.

Specifically, internal changes include a decrease in the secretion of various hormones that regulate metabolism, and a decrease in the function and activity of immune cells, resulting in a weakened immunity. In addition, external effects include increased frequency of exposure to ultraviolet rays due to the destruction of the ozone layer, long-term exposure to blue light, a high-energy visible light that is frequently emitted from electronic devices such as TVs, PCs, and mobile phones, and air pollution. As it gets worse, skin wrinkles increase due to the effects of free radicals and active harmful oxygen, skin elasticity decreases, and spots, freckles, and age spots tend to increase. Accordingly, a variety of methods to overcome skin aging have recently been studied.

The epidermis, located in the outermost layer of human skin, protects against physical and chemical irritants such as various external stimuli such as chemicals, air pollutants, dry environments, and ultraviolet rays, and provides moisture to the body through the skin. It performs a protective function to prevent excessive dissipation of This protective function can be maintained only when the stratum corneum, composed of keratinocytes, is normally formed.

Meanwhile, the stratum corneum (horney layer), which is the outermost layer of the epidermis, is formed from keratinocytes and consists of fully differentiated keratinocytes and a lipid layer surrounding them, and keratinocytes continuously proliferate in the lowest layer of the epidermis. ) is a characteristic cell that rises to the surface of the skin through gradual changes in form and function. After a certain period of time, old keratinocytes are shed from the skin and new keratinocytes take over their functions. This repetitive series of changes is called “epidermal cell differentiation” or “keratinization.”

During the keratinization process, keratinocytes produce Natural Moisturizing Factor (NMF) and intercellular lipids (ceramide, cholesterol, and fatty acids) to form a stratum corneum, making the stratum corneum firm and flexible, forming a skin barrier. ) to retain the function.

However, modern people tend to have an increase in dry skin due to damage to the function of the stratum corneum due to lifestyle factors such as excessive washing of the face, environmental factors such as dry atmospheric conditions, and endogenous diseases. Therefore, there is a need for skin moisturizers to solve this problem. It is increasing.

Polyhydric alcohols and sugars have been used as skin moisturizers in cosmetics as active ingredients in general moisturizers. However, it has the disadvantage of causing stickiness on the skin, not providing sufficient moisturizing effect to the skin, and also not providing a continuous moisturizing effect. Furthermore, in the summer when humidity is very high, moisture is drawn from the outside and the skin becomes sticky. And in winter, the humidity in the outside atmosphere is low, which causes problems that actually draw moisture from the skin, causing the skin to become dry.

Meanwhile, human skin is sensitive to relative humidity, and usually feels comfortable when the relative humidity is 40 to 70%. The relative humidity is a value expressed in % as the ratio of the amount of water vapor contained in 1 m ³ of air to the amount of saturated water vapor at the same temperature. In Korea, the humidity is very high and humid in the summer, and the air is very dry in the winter due to the low relative humidity.

Human skin is also greatly affected by seasonal changes and changes in humidity. Discomfort increases in the summer when the relative humidity is high due to high temperature and humidity, and in the dry winter when the relative humidity is low, the lips and skin also become dry, cracked, and sometimes have difficulty breathing. I also feel it. When the relative humidity is high, it absorbs moisture in the air and becomes sticky. Conversely, when the relative humidity is low, the moisture in the skin evaporates and becomes dry. Especially when the relative humidity is low, the dryness of the skin is accompanied by tightness and itching. There are a lot.

Accordingly, research in various fields of cosmetics has attempted to improve dryness of the skin by using various moisturizing ingredients such as oil, sugar, and polyol to overcome dryness of the skin.

Specific examples include suppressing moisture evaporating from the skin by strengthening the skin barrier function using skin components such as ceramide, cholesterol, and fatty acids, or improving skin moisture retention ability by using sugars and polyols that have a strong ability to bind moisture on the skin surface. There have been attempts to increase the moisturizing effect of the skin by strengthening the skin. However, it is difficult to maintain the effect for a long time in the winter when the relative humidity is low, and it causes discomfort in the summer when the relative humidity is high, making it difficult to manufacture an appropriate cosmetic composition according to changes in relative humidity.

Meanwhile, in the cosmetics industry, the use of animal raw materials that can cause mad cow disease has recently been banned, so there is a trend not to prefer the use of animal raw materials. Accordingly, customer preference for plant-based raw materials has increased relatively. According to this trend, the use of herbal medicine extracts has increased. A lot of research is being done to use it as a raw material for cosmetics.

As a specific technology, Republic of Korea Patent No. 10-1724146 is a cosmetic composition for removing dead skin cells containing herbal extracts, including centella asiatica extract, goldenrod extract, chamomile extract, licorice extract, green tea extract, Japanese knotweed extract, and water parsley extract. It relates to a cosmetic composition for removing dead skin cells containing the active ingredient and the remaining purified water. Containing herbal extracts, it removes dead skin cells and regulates the cycle without irritating even sensitive skin, while enhancing skin soothing and moisturizing effects. A cosmetic composition that can more effectively normalize the skin is disclosed.

However, although the above prior art has the advantage of minimizing skin irritation by using natural products, it is unable to achieve a moisturizing effect above a certain level.

Accordingly, the present applicant developed a composition containing daylily extract, a natural extract, as a composition to solve the above problems, minimize skin irritation, and maximize skin moisturizing effect.

KR 10-1724146 B1

The purpose of the present invention is to provide a skin moisturizing composition containing daylily extract as an active ingredient, which does not cause skin irritation and has excellent skin moisturizing effect.

Another object of the present invention is to provide a cosmetic composition, which does not cause skin trouble when applied to the skin, does not cause skin irritation, and contains ingredients derived from natural substances such as daylily extract, so that it can be used even in cases of environmental skin diseases. The aim is to provide a composition that is possible and can exhibit excellent skin moisturizing effects.

Another object of the present invention is to provide a composition that can be provided as a food composition and exhibit a skin moisturizing effect upon ingestion.

In order to achieve the above object, a composition for moisturizing skin according to an embodiment of the present invention includes daylily extract as an active ingredient.

The composition does not cause skin irritation and has excellent skin moisturizing effect.

The extract is extracted using any one selected from the group consisting of water, lower alcohols having 1 to 6 carbon atoms, and mixtures thereof as an extraction solvent.

The composition may further include fermented seaweed extract.

A cosmetic composition for moisturizing skin according to another embodiment of the present invention includes the composition for moisturizing skin.

The cosmetic composition may be a formulation selected from the group consisting of skin, serum, lotion, emulsion, cream, essence, lotion, foundation, pack, soap, surfactant-containing cleansing, spray, and powder.

A functional food composition for skin moisturizing according to another embodiment of the present invention includes the skin moisturizing composition.

Hereinafter, the present invention will be described in more detail.

In the human body, the skin is an organ that functions as a protective film that protects the living body from the external environment. It prevents the loss of biological components in the human body, such as water and electrolytes, and also prevents harmful substances from entering the human body from the outside. do.

This skin is largely divided into the epidermis layer, dermis layer, and subcutaneous fat layer. The epidermal layer is composed of keratinocytes and melanocytes. The outermost keratinocyte layer of the skin is in direct contact with the external environment, so it requires strong resistance to physical, chemical, or material penetration, and prevents moisture loss from the human body to the outside. It must maintain flexibility by retaining adequate moisture while simultaneously shrinking.

In general, the moisture content of the skin is about 70% in the dermis layer, but decreases toward the epidermis layer, reaching about 10 to 30% in the keratinocyte layer. Water supplied from the dermal layer mainly moves to the upper part of the stratum corneum by passive diffusion and is ultimately discharged to the outside.

This is called Trans Epidermal Water Loss (TEWL), and it is known that the sebum component and epidermal lipid, which are lipid membrane components of the keratinocyte layer, maintain this transepidermal water loss at an appropriate level. In addition, it is known that the keratinocyte layer contains a hydrophilic substance with water-retaining ability called Natural Moisturizing Factor (NMF), which plays an important role in moisturizing the skin.

When the moisture content of the normal keratinocyte layer is maintained at about 10 to 30%, the skin is smooth and soft and functions to protect the body normally. However, when the moisture content of the keratinocyte layer falls below 10%, the skin becomes rough and loses its protective function, causing aging.

For example, in the case of dry skin, the cohesion of keratinocytes is weakened and a scaling phenomenon occurs in which keratinocytes peel off from the skin surface like scales. This drying of the skin is due to the moisture content of the keratinocyte layer. This is because it is less than normal skin.

In addition, even if the skin is healthy, the skin condition may worsen due to lack of moisture due to harsh external environments, such as wind, cold weather, sunlight, washing the face, and shaving.

Therefore, it is very important to maintain the appropriate moisture content of the skin's keratinocyte layer. It is known that when the moisture content of the stratum corneum is less than 10%, the skin becomes dry and causes skin problems. The amount of moisture in the stratum corneum created by regular keratinization is always maintained at 10 to 20%.

Currently, occlusive agents are used to retain moisture in the skin. Occlusive agents are hydrophobic substances that promote moisture retention by forming a barrier on the skin that prevents moisture loss.

The most commonly used occlusives include petrolatum, lanolin, cocoa butter, mineral oil, and silicone. In addition, to make cosmetics, 20 to 50 different raw materials are generally used, such as sodium lauryl sulfate, dimethyl sulfoxide, croton oil, sodium hydroxide, etc. When these raw materials are applied to products, they can cause skin irritation such as inflammation, itching, and allergies.

The main characteristic of the composition of the present invention is that it does not cause skin irritation when applied to the skin and exhibits an excellent skin moisturizing effect.

A skin moisturizing composition according to an embodiment of the present invention includes daylily extract as an active ingredient.

The daylily (Hemerocallis fulva L) is a general name for perennial grasses of the genus Daylily of the family Daylily and is a perennial grass with long flower tubes and flowers similar to lilies. The leaves are linear and alternate in two rows, and the back part is curved backward like a bow. The young shoots are eaten as a vegetable, and the dried flowers are used in Chinese cuisine. Additionally, the root is used as a diuretic, hemostatic, and anti-inflammatory agent.

The extract may be extracted using any one selected from the group consisting of water, lower alcohols having 1 to 6 carbon atoms, and mixtures thereof as an extraction solvent.

Specifically, grinding dried daylily leaves; leaching the pulverized material using an organic solvent; Leaching and drying the sample; Re-leaching the dried sample using an organic solvent; Leaching and drying the sample; Leaching using water; And including the step of leaching, a daylily extract can be obtained.

The step of fractionating the daylily extract extracted using the organic solvent may be further included.

The method of preparing the extract may be a conventional extraction method in the art, such as ultrasonic extraction, leaching, and reflux extraction. Specifically, it may be an extract obtained by extracting a natural product from which foreign substances have been removed by washing and drying with water, alcohol with 1 to 6 carbon atoms, or a mixed solvent thereof, or it may be an extract obtained by sequentially applying the solvents to the sample.

The reflux extraction method is based on 100 mL of alcohol having 1 to 6 carbon atoms, 10 to 30 g of pulverized natural product, a reflux time of 1 to 3 hours, and 50 to 100% of alcohol having 1 to 6 carbon atoms. More specifically, based on 100 mL of alcohol with 1 to 6 carbon atoms, 10 to 20 g of pulverized natural product, reflux time of 1 to 2 hours, and 70 to 90% of alcohol with 1 to 4 carbon atoms.

The leaching method is performed at 15 to 30° C. for 24 to 72 hours and 50 to 100% of alcohol with 1 to 6 carbon atoms. More specifically, at 20 to 25° C., for 30 to 54 hours, and 70 to 80% of alcohol having 1 to 6 carbon atoms.

The ultrasonic extraction method is performed at 30 to 50° C. for 0.5 to 2.5 hours and 50 to 100% alcohol with 1 to 6 carbon atoms. Specifically, at 40 to 50° C. for 1 to 2.5 hours and 70 to 80% of alcohol having 1 to 6 carbon atoms.

The extraction solvent can be used in an amount of 2 to 50 times, more specifically, 2 to 20 times the weight of the sample. For extraction, the sample may be left for 1 to 72 hours, more specifically, 24 to 48 hours for leaching in the extraction solvent.

After extraction, the extract can be fractionated by sequentially applying a new fractionation solvent. The fractionating solvent used during fractionation is at least one selected from the group consisting of water, hexane, butanol, ethyl acetic acid, ethyl acetate, methylene chloride, and mixtures thereof, and is preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, additional methods such as concentration or freeze-drying can be used.

The composition may further include fermented seaweed extract. The skin moisturizing composition can be provided as a moisturizing composition with excellent moisturizing effect and high preference even when it contains only daylily extract, but can exhibit increased moisturizing effect by mixing with fermented seaweed extract and is a cosmetic composition with high preference or It makes provision as a food composition possible.

In addition, when the fermented seaweed extract is used as is, there is a problem in that the preference is lowered due to the unique off-flavor of the seaweed. To prevent this problem, extracting active ingredients from fermented seaweed and mixing them with daylily extract not only increases the moisturizing effect but also makes it possible to provide a composition with high preference.

The seaweed extract is specifically, seaweed extract, green seaweed extract, cap extract, seaweed extract, hijiki extract, seaweed extract, seaweed extract, kelp extract, Ecklonia cava extract, Agar extract, Agar extract, chlorella extract, Salicornia extract, Agar extract, and rhubarb. It may be selected from the group consisting of extracts and mixtures thereof, and preferably may be selected from the group consisting of Siberian lanceolate extract, Semogasari extract, and mixtures thereof, but is not limited to the above examples.

Gracilaria verrucosa is a type of red seaweed that belongs to the family Gracilaria verrucosa and grows attached to stones and shells in the intertidal zone of Korea, Japan, Sakhalin Island, and Taiwan. In particular, it breeds on pebbles or piles in shallow beaches where river water flows into the sea, and also grows on reefs in the open sea. The body grows from small tray-shaped roots and has a cylindrical shape. It is densely divided into pinnate shapes, and the branches sometimes grow out to one side. The branches extending from the body are wire-shaped cartilaginous with a diameter of less than 1 mm, blackish purple or dark brown, 1-3 mm in diameter, and grow to a cylindrical length of 2-3 m. They are slightly boiled and eaten as a seasoning or agar. It is also used as a raw material.

The Gloiopeltis furcate is a reddish-brown annual seaweed, which means that the stem is thin in the middle of the seaweed. Semogasari is 5 to 15 cm tall, has a cylindrical body, branches slightly irregularly, and its body shape varies greatly depending on where it grows. The branches are hollow, the branching points are narrow, and the width of the branches reaches 4 mm. Semogasari grows luxuriantly throughout winter and spring, matures in early summer and produces spores.

For the seaweed extract presented above, the washed seaweed is placed in a fermentation vessel and inoculated with microorganisms to proceed with the fermentation and maturation process.

The microorganisms include Lactobacillus salivarius, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus rhamnosus, Lactobacillus plantarum, and lactobacillus. Lactobacillus helveticus, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus reuteri ( Lactobacillus, Lactococcus, such as Lactobacillus reuteri, Lactobacillus buchneri, Lactobacillus gasseri, Lactobacillus johonsonii, Lactobacillus kefir, etc. lactis), Lactococcus plantarum, Lactococcus raffinolactis, Enterococcus faecalis, Enterococcus faecium, Streptococcus thermophilus, Leuconostoc lactis, Leuconostoc mesenteroides, etc. and Bifidobacterium animals, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium pseudolongum, Bifidobacterium thermophilum It may include bifidobacteria such as (Bifidobacterium themophilum), Bifidobacterium adolescentis, etc., and more preferably Lactobacillus casei, Lactobacillus rhamnosus, and Bifidobacteria. Bifidobacterium bifidum may be selected from the group consisting of Bifidobacterium, Bifidobacterium breve, and Lactobacillus acidophilus, but may be selected from the group consisting of the above microorganisms. It is not limited and can be used without restrictions.

After inoculating the microorganism, a fermentation and maturation process is performed at 30 to 35° C. for 1 to 2 days. Once the fermentation and maturation process is completed, the fermented seaweed extract is produced by the extract production process described above.

The composition may include 20 to 40 parts by weight of fermented Achyranthes extract and 20 to 40 parts by weight of fermented Siberian root extract, based on 100 parts by weight of the daylily extract. When mixed and used within the above range, skin irritation does not occur, moisturizing effect is relatively improved, and skin applicability can be improved.

A cosmetic composition for moisturizing skin according to another embodiment of the present invention includes the composition of the present invention.

The cosmetic composition of the present invention can be prepared by additionally mixing the composition of the present invention with a dermatologically acceptable carrier.

The dermatologically acceptable carrier may include purified water, oil, wax, fatty acid, fatty alcohol, fatty acid ester, surfactant, moisture absorbent, thickener, antioxidant, viscosity stabilizer, chelating agent, buffer, preservative, lower alcohol, etc. However, it is not limited to this, and its types and concentrations are diverse, and includes parts that can be changed by those skilled in the art within the scope of the present invention.

In addition, functional substances such as whitening agents, moisturizers, anti-inflammatory agents, antibacterial agents, antifungal agents, vitamins, sunscreens, antibiotics, anti-acne agents, perfumes, and dyes may be included as needed, and these may be included in amounts commonly used in the cosmetics field. It can be included in the cosmetic composition according to the present invention. In order to increase its functional effect, the cosmetic of the present invention may contain one or more types of moisturizing active ingredients that exhibit the same or similar functions as the composition of the present invention.

Cosmetics prepared from the cosmetic composition of the present invention can be manufactured in the form of general emulsified formulations and solubilized formulations. Cosmetics in emulsified formulations include nourishing lotions, creams, and essences, and cosmetics in solubilized formulations include flexible lotions.

In addition to the composition of the present invention, the cosmetic product may also be manufactured in the form of an auxiliary agent commonly used in the art for topical or systemic application by containing a dermatologically acceptable medium or base.

Suitable cosmetic formulations include, for example, solutions, gels, solid or pasty anhydrous products, emulsions obtained by dispersing the oil phase in the aqueous phase, suspensions, microemulsions, microcapsules, microgranules or in ionic (liposome) or non-ionic form. It may be provided in the form of a vesicular dispersion, cream, skin, lotion, powder, ointment, spray or conceal stick. Additionally, it can be prepared in the form of foam or in the form of an aerosol composition further containing compressed propellant.

Products to which the cosmetic composition of the present invention can be added include, for example, cosmetics such as astringent lotion, softening lotion, nourishing lotion, various creams, essences, packs, foundations, etc., and cleansers, face washes, soaps, treatments, or serums. etc. Specific formulations include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, essence, nutritional essence, pack, soap, shampoo, cleansing. It includes formulations such as foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, pressed powder, loose powder, or eye shadow.

A food composition according to another embodiment of the present invention includes the skin moisturizing composition.

At this time, the amount of the extract in the food or beverage can be added at about 0.01 to 15% by weight of the total weight of the food, and the health drink composition can be added at a rate of about 0.01 to 10g based on 100 ml, but is not limited thereto. .

When the food composition of the present invention is a beverage composition, other than containing the extract as an essential ingredient in the ratio indicated, there are no particular restrictions on other ingredients, and various flavoring agents or natural carbohydrates may be contained as additional ingredients like ordinary beverages. You can. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose, etc.; Disaccharides such as maltose, sucrose, etc.; and polysaccharides, such as common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (thaumatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used, but are not limited to these. The ratio of the natural carbohydrate may generally range from about 1 to 20 g per 100 ml of the composition of the present invention, but may not be limited thereto.

In addition to the above, the composition of the present invention contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its It may contain salt, organic acid, protective colloidal thickener, pH adjuster, stabilizer, preservative, glycerin, alcohol, carbonating agent used in carbonated beverages, etc., but may not be limited thereto.

In addition, the composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks, and these ingredients can be used independently or in combination. The proportion of these additives is not critical, but may be selected in the range of about 0.1 to about 20 parts by weight per 100 parts by weight of the composition of the present invention, but is not limited thereto.

A functional food composition according to another embodiment of the present invention may include the skin moisturizing composition.

Functional foods as referred to in the present invention include health supplements, which are foods manufactured and processed using specific ingredients as raw materials or specific ingredients contained in food ingredients by methods such as extraction, concentration, purification, and mixing for the purpose of health supplementation, In addition, it is a food designed and processed to fully exert the bioregulatory functions of food ingredients, such as biodefense, regulation of biorhythm, prevention and recovery of disease, etc., and also has functions related to disease prevention and recovery from disease. Make sure to include everything.

The present invention relates to a skin moisturizing composition containing daylily extract as an active ingredient, which does not cause skin irritation and has an excellent skin moisturizing effect. It is provided as a cosmetic composition, and does not cause skin irritation when applied to the skin, preventing skin troubles. It does not cause skin irritation, can be used even in cases of environmental skin diseases as it contains ingredients derived from natural substances, and can have an excellent skin moisturizing effect.

Additionally, it can be provided as a food composition and have a skin moisturizing effect upon ingestion.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement it. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.

Manufacturing example

Preparation of daylily extract

After obtaining only the daylily leaves, they were washed, dried, pulverized to make pulverized products, and freeze-dried. 1 mg of 3 g of freeze-dried daylily leaves was placed in a 15 ml tube, and 10 ml of 70% ethanol was added again. Samples were mixed and extracted in a rounded mixing machine for 18 hours. After extraction, centrifugation at 3000 rpm for 10 minutes was performed, and only the supernatant was collected. Ethanol contained in the supernatant collected in this way was evaporated at 40°C. And the remaining samples were freeze-dried. Daylily extract (PE) used in this experiment was extracted from daylily raw materials with a yield of 11.5%.

Preparation of fermented seaweed extract

After drying the sea snail, it was pulverized using a grinder. Fermented yellowtail was produced by inoculating the pulverized yellowtail with Lactobacillus rhamnosus and fermenting it at 25°C for 3 days. Afterwards, fermented Brassica serrata extract (GE) was prepared in the same manner as the preparation method of daylily extract.

Fermented Siberian snail extract (FE) was prepared using the same method as the fermented Siberian snail extract.

Preparation of mixed extract

Day lily extract (PE), fermented snail extract (GE), and fermented cypress extract (FE) were mixed and used as shown in Table 1 below.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative example 2 PE 100 - - 100 100 100 100 100 GE - 100 - 20 30 40 10 50 F.E. - - 100 20 30 40 10 50

(unit weight)

Experimental Example 1

skin irritation test

The irritation-relieving effect was confirmed through a human skin patch test on 15 women in their 20s and 30s who had never shown hypersensitivity to skin irritation in the past and currently had no symptoms of skin disease or skin allergy. Examples and comparative examples in which 1.0% by weight of lactic acid, a skin irritant, was added as an irritant were used.

First, the participant's test was washed with 70% by weight ethanol and dried. 15 ㎍ of the prepared test substance was dropped into a Finn chamber (100x10, EPITEST, Finland) and then sealed and applied to the inside of the test subject's forearm. The patch was applied for 24 hours, the patch was removed, and the test area was marked with a marking pen. One hour and 24 hours after marking, the test area was observed using a magnifying glass (8MC-150, DAZOR, USA) to observe the presence or absence of erythema and edema.

Skin reactions were determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) in Table 2, and the results are shown in Table 3.

sign score Evaluation standard - 0 No response ± 0.5 faint or mild erythema + One Erythema, edema, and papules have clear boundaries but are mild. ++ 2 Marked erythema, papules, and vesicles +++ 3 Severe erythema, blisters, and crust formation

[Equation 1]

Mean skin response rate = (score x number of reactions x 100 x 1/2)/[3 (maximum score) x total number of subjects (n)]

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative example 2 Reactivity (%) 3.11 3.98 4.33 2.33 2.01 2.44 7.11 7.56

According to the above experimental results, it was confirmed that skin irritation did not occur when the daylily extract was used, and that skin irritation did not occur significantly even when the fermented Corolla extract and the fermented Semogasari extract were used. However, in the case of complex extracts, it was confirmed that when mixed and used within a certain range, the best skin irritation relief effect was observed due to the synergistic effect of the mixed use of extracts.

Experimental Example 2

Moisturizing effect experiment

Measurement of water retention capacity The electrical conductivity of the skin surface was measured using a Corneometer (Courage + Khazaka, GmbH, Germany), and the measurement temperature was 25°C and 45% relative humidity in a constant temperature and humidity room maintained in the Examples and A certain amount (0.03g/16cm ² ) of each cosmetic prepared in the comparative example was applied to the inner side of the lower arm of 15 test subjects, and the moisture content of the skin was measured before and 2 hours after application. Normal skin without any treatment was used as the control group. It was used as. The results are shown in Table 4 below.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative example 2 Before application 50 50 50 50 50 50 50 50 2 hours later 73 59 60 85 94 88 40 45

According to the above experimental results, it was confirmed that when daylily extract was used, an excellent skin moisturizing effect was observed even after 2 hours, and when a complex extract was used, an excellent skin moisturizing effect was observed within a certain range.

Experimental Example 3

Skin applicability evaluation

A cosmetic cream was prepared using the compositions of the examples and comparative examples above, and its spreadability was evaluated.

3% by weight of each of the above Example and Comparative Example compositions, 1% by weight of magnesium ascorbic acid-2-phosphate, 1% by weight of water-soluble collagen (1% aqueous solution), 0.01% by weight of sodium citrate, 0.05% by weight of citric acid, 1,3- A cream was prepared by mixing 3% by weight of butylene glycol and the remaining purified water. Six products were provided to 15 women in their 20s and 30s, and then a relative evaluation of their applicability to the skin was requested. Evaluation was requested from 1 to 5 points, with higher scores indicating better products.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative example 2 Applicability to skin 3 2 2 4 5 4 One One

According to the above evaluation results, the cream containing only daylily extract was evaluated as having medium spreadability, but when the complex extract was used, it showed very excellent spreadability, increasing the possibility of providing the cream with a high preference.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present invention defined in the following claims are also possible. falls within the scope of rights.

Claims (7)

Based on 100 parts by weight of daylily extract, it contains 20 to 40 parts by weight of fermented Achyranthes extract and 20 to 40 parts by weight of fermented Cypress extract as active ingredients,
The fermented snail extract and the fermented snail extract are fermented with Lactobacillus rhamnosus strain.
Composition for skin moisturizing. According to paragraph 1,
The composition does not irritate the skin and has an excellent skin moisturizing effect.
Composition for skin moisturizing. According to paragraph 1,
The extract is extracted using any one selected from the group consisting of water, lower alcohols having 1 to 6 carbon atoms, and mixtures thereof as an extraction solvent.
Composition for skin moisturizing. delete Containing the skin moisturizing composition according to any one of claims 1 to 3.
Cosmetic composition for skin moisturizing. According to clause 5,
The cosmetic composition is a formulation selected from the group consisting of skin, serum, lotion, emulsion, cream, essence, lotion, foundation, pack, soap, surfactant-containing cleansing, spray, and powder.
Cosmetic composition for skin moisturizing. Containing the skin moisturizing composition according to any one of claims 1 to 3.
Functional food composition.