KR101880470B1 - Cosmetic composition comprising organic acid fermentation and natural polymer for moisturizing effect on the skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for moisturizing skin containing an organic acid fermentation broth and natural polymer components, wherein the organic acid fermentation broth is a mixture of Acetobacter sp. Or Lactobacillus sp. Strain in a mixture of plum, citron, pomegranate, , And the natural polymer component is beta-glucan or natto gum. According to the present invention, a cosmetic composition containing an organic acid fermentation broth and a natural polymer component is excellent in moisturizing effect and skin barrier improving effect, and has excellent feeling of use.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for skin moisturizing, which comprises an organic acid fermentation broth and a natural polymer component. [0002]

The present invention relates to a cosmetic composition for moisturizing containing an organic acid fermentation broth and a natural polymer component.

As nowadays, it is possible to control the temperature of the air and / or heating by changing the environment or the life pattern, the skin stress due to various stresses and environmental pollution generated in the social life, frequent cleansing according to makeup habit, Aging and the like, moisture of the stratum corneum is reduced, and the skin becomes dry, the surface becomes rough, the skin becomes loose, the luster is lost and the skin becomes dull, and the necessity of the skin moisturizing agent is increasing.

The cosmetic compositions contain various moisturizing agents such as oils, sugars and polyols to improve the dryness of the skin. Humectants which have a property of absorbing moisture as a moisturizing agent and Occlusive Moisturizers which prevent water evaporation have been used to increase the moisture retention on the skin surface.

As the humectant, there are substances such as glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, and sorbitol, but they have a disadvantage that they are sticky or worn out when they are applied to the skin. As a closed moisturizer, lipid components such as ceramides and cholesterol, and essential fatty acids and lipid complexes have been used (J. Invest. Dermatol. (5), 731-470, 1994), but it is difficult to maintain the stability of emulsified formulations. There is a disadvantage in that it is not suitable for producing a product on the surface. Also, in the case of a product having a good moisturizing power, stickiness is caused, which may cause discomfort when the feeling of use is bad or severe.

In recent years, it has been suggested that the differentiation of the stratum corneum is important for preventing the drying of skin moisture, and it is suggested that the keratinous vertical developmental differentiation process of the stratum corneum of the outermost stratum in the basal layer kerosene formation can be performed normally, The facts turned out. In other words, during the process of keratinization, the cells produce natural moisturizing factors and intercellular lipids, and as a result, the stratum corneum becomes rigid and supple, allowing it to function as a barrier. Physiological tendency of the skin to become dry as the age of the human is increased can be interpreted as a longer time for the removal of the stratum corneum, a lowering of the lipid synthesis ability of the epidermal cells, and a decrease in the amount of the moisturizing factor and lipid in the stratum corneum .

Korean Patent No. 10-1307969 relates to a cosmetic composition comprising a fermentation liquid obtained by fermenting a mixed liquid prepared by mixing fruit (plum, citron, pomegranate) and vinegar.

An object of the present invention is to provide a cosmetic composition for humidification which promotes skin turnover, attracts moisture and prevents skin moisture evaporation, thereby enhancing skin barrier, improving skin moisture retention, and having excellent skin moisturizing effect and tactile feeling .

The present invention relates to a cosmetic composition for moisturizing skin containing an organic acid fermentation broth and a natural polymer component, wherein the organic acid fermentation broth is selected from the group consisting of Acetobacter sp., Lactobacillus sp. Or a mixed strain thereof, and the natural polymer component is beta glucan or natto gum.

Preferably, the organic acid fermentation broth may be contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the natural polymer component may be contained in an amount of 0.00001 to 20.0% by weight based on the total weight of the cosmetic composition.

Preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray ≪ / RTI >

The cosmetic composition according to the present invention is superior in moisturizing effect (Experimental Example 1) and skin barrier improving effect (Experimental Example 2) and excellent in feeling of use (Experimental Example 3).

In order to achieve the above object, the cosmetic composition for skin moisturizing of the present invention is characterized by containing an organic acid fermentation broth and a natural polymer component as an effective ingredient.

Hereinafter, the present invention will be described in more detail.

The organic acid fermentation broth used as an active ingredient in the present invention is composed of components fermented with plum, citron, pomegranate and vinegar. Specifically, a mixture of Acetobacter sp. Or Lactobacillus Lactobacillus) strain.

Prunus mume Sieb. Et Zucc. (Japanese apricot) is a fruit plant of Rosaceae and its origin is mainly cultivated in Japan, Taiwan, China and Korea from central to southern China. Fruit contains 4 ~ 5% of acid and is used for processing almost because it has strong acidity. The main ingredient of fruit is citric acid, malic acid, less than 1% sugar. 85% of pulp is rich in moisture, 10% is carbohydrate, rich in minerals and organic acids, and contains carotene. Alkaline food is good for fatigue recovery and is effective in improving the constitution. It is excellent in detoxification and helps to stomach and food poisoning. It is also known as anticancer food.

Citrus junos Tanaka (Chinese lemon) is the origin of the upper Yangtze River in China and its distribution is wide. In Korea, it is grown in the southern coastal area and part of the west coast. Fruit is 100 ~ 130g, single spherical, thick in the skin, yellow on the undersurface, dented, deep irregularities. The fruit has a unique flavor, the flesh is soft and juicy, and the acid content is high. It is processed not only with vinegar but also with citron, citron. The distinctive aroma of citron is due to the amount of essential oil ingredients and the main ingredients are 30 to 40 species such as limonene, β-pinene, linalool, citronellol, . Vitamin C contains more than 150mg per 100g, more than three times lemon or navel. Yuzu has been used in the treatment of colds, neuralgia, wind, nausea, vomiting, indigestion, and poisoning, and it is said to be effective in cancer prevention. In addition, contains the same effect as vitamin P Hesperidin to protect the capillaries, vitamin B complex and vitamin A, the mother of carotene content is high.

The pomegranate (Punica granatum L.) is 10 meters tall and the stem is twisted. Iran is the origin of Afghanistan, and it is estimated that it was introduced to Korea about 500 years ago. Flowers are bisexual, and pink in June. Fruits are round and have a diameter of 6-8, and ripen in yellow or yellowish pink in September-October. There are red seeds in the inside. When they are fully ripe, the outer shell grows irregularly and seeds inside can be seen. The seeds are tasted and strained, and they eat as they are, or they make green leaves. In addition, the peel has the efficacy of convergence, hemostasis, branch, and antiparasitic effect, and in the oriental medicine, it has the effect of kusari, hematemesis, subarachnment, blob, (Glucose, fructose), about 40% of pomegranate, and about 1.5% of citric acid which gives a sour taste of organic acid. Water-soluble vitamins (B1, B2, niacin), but the amount is low, the skin is tannin, seeds are known to contain natural vegetable estrogens good for menopausal disorders.

Vinegar is a vinegar made by fermenting brown rice for more than a year. It is a vinegar made in Jinjiang, southern China. After long fermentation and ripening period, dark vinegar is produced. It has weak acidity, moderate sweetness and aroma, so it is not burdensome to drink as it is. Vinegar contains a lot of amino acids compared to other vinegar, this amino acid prevents the blood sugar from changing to fat, it is also known to have an effect on the diet, helping to consume blood sugar in the muscle.

The organic acid fermentation broth means fermentation with a mixture of plum, citron, pomegranate and black vinegar with an Acetobacter sp. Strain, a Lactobacillus sp. Strain or a mixed strain thereof. Specifically, citron juice, plum juice, pomegranate juice, and vinegar were added to prepare a mixed solution. Then, a mixture of acetobacterium and / or lactobacillus was added at a concentration of 105 to 107 cfu / ml in a ratio of 9: 1 And cultured at room temperature for 7 days. After culturing, the culture broth was centrifuged to remove the culture bacteria first, and the filtrate thus obtained was finally filtered through a 0.25 uM filter.

The strain of the genus Acetobacter is selected from the group consisting of acetobacter aceti, Acetobacter xylinum, Acetobacter pasteurianus, and Acetobacter hansenii. And may be preferably acetobacter aceti.

The strain of the genus Lactobacillus is selected from the group consisting of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus rhamnosus, Lactobacillus flamarium, plantarum, Lactobacillus reuteri, Lactobacillus confusus, Lactobacillus fermentum, Lactobacillus brevis, and Thermus thermophillus. May be selected and used, and preferably, it may be Lactobacillus acidophilus.

The organic acid fermentation broth may be contained in an amount of 0.001 to 30.0% by weight, preferably 0.01 to 20.0% by weight, based on the total weight of the cosmetic composition. When the content of the organic acid fermentation broth is less than 0.001% by weight, it is difficult to obtain a desired effect. When the content of the organic acid fermentation broth is more than 30.0% by weight, the degree of increase of the skin improving effect against the increase of the content is insignificant and the possibility of inducing skin irritation is high. There is a problem with stability and it is not economical.

The natural polymer component used in the present invention is one of beta-glucan or natto gum.

As a natural polymer, beta-glucan contains a glycosidic linkage of a D-glucose monomer as a glucose polymer, and is classified into alpha-glucan and beta-glucan depending on the position where glucose is bonded. Glucan has been shown to have anti-cancer effects by activating natural killer cells and lowering the levels of total cholesterol and low density lipoprotein cholesterol (LDL-C) in the blood. Beta glucan has the effect of lowering the concentration of low-density lipoprotein cholesterol. This is because beta-glucan binds to bile acid to increase intestinal viscosity and reduce reabsorption of bile acid. As a result, it increases the synthesis and excretion of bile acids in the intestines, and at the same time increases the synthesis of cholesterol in the liver. In addition, alpha-glucan can be obtained from starch of plants, and beta-glucan can be obtained from cell walls of mushrooms, grains and yeast.

In particular, beta-1, 3-glucan used in the present invention is a high-purity glucan having a purity of 98% or higher obtained by liquid culture of a mycelium of Schizopyllum commune. In addition to skirt mushroom, Beta-1,3-glucan obtained by culturing yeast, barley and the like can be used.

Natto used in the present invention is a natural polymer component obtained by culturing microorganisms in black soybeans, which is a main ingredient of glutamic acid and fructose, is excellent in moisturizing power and excellent in lubricity.

The natural polymer component may be 0.00001 to 20% by weight, preferably 0.001 to 10% by weight, based on the total weight of the cosmetic composition. When the content of the natural polymer component is less than 0.00001% by weight, the effect on the skin is insufficient. When the content of the natural polymer component is more than 20% by weight, the degree of increase in skin moisturizing effect is insufficient. In particular, in the case of natto gum, if it exceeds 20% by weight, it is difficult to dissolve completely in water and it is not suitable because it shows a sticky feel.

The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, and may contain conventional ingredients such as antioxidants, stabilizers, solubilizers, vitamins, Supplements, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powdered foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Production Example 1: Production of organic acid fermentation broth

In the organic acid fermentation broth used in the present invention, citron, plum, and pomegranate were selected as fruit samples, washed thoroughly, and crushed to make fruit juice. Then, a mixture was prepared by adding citron juice: plum juice: pomegranate juice: black vinegar at a weight ratio of 40: 30: 10: 20. Lactobacillus acidophilus KTCT 1010 and Lactobacillus acidophilus KTCT 3140 were inoculated at a concentration of 105 to 107 cfu / ml at a ratio of 9: 1 to the completed mixture, Lt; / RTI > After culturing, the culture broth was centrifuged to remove the culture bacteria first, and the filtrate thus obtained was finally filtered through a 0.25 uM filter.

Example  And Comparative example  Produce

Examples 1 and 2 are the formulations containing the organic acid fermentation broth and beta glucan or natto gum prepared in the above Preparation Example as shown in Table 1 below. Beta Glucan used in the present invention and PF of Natto black SK Bioland Company) Betaglucan marketed under the trade name SC-Glucan and Natto gum marketed under the trade name Soypol SP were used. Comparative Examples 1 to 3 each contain an organic acid fermentation broth, beta-glucan, and natto gum, and Comparative Example 4 does not include components related to the present invention.

ingredient Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Organic acid fermentation broth
(Production Example 1) 5.0 5.0 10.0 - - - Beta Glucan 5.0 - - 10.0 - - Natto sword - 5.0 - - 10.0 - glycerin 3.0 3.0 3.0 3.0 3.0 3.0 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 Purified water to 100 to 100 to 100 to 100 to 100 to 100 Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.7 0.7 0.7 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 5.0 5.0 5.0 Trioctanoin 5.0 5.0 5.0 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.2 0.2 0.2 0.2 0.2 0.2 Cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Experimental Example 1: Moisturizing effect 1 - Moisturizing effect and persistence

The clinical moisturizing effect and the sustaining ability of the cosmetic compositions of Examples 1 and 2 and Comparative Examples 1 to 4 were measured as follows. A questionnaire survey was conducted to 30 healthy adults and women who felt skin dry to apply the cosmetic compositions of Examples 1 and 2 and Comparative Examples 1 to 4 to the upper arm of the arm twice a day for 4 weeks. The moisturizing effect was evaluated by changing the electrical conductivity according to the moisture content of the epidermis using Corneometer CM 820 (Corage + Khazaka, Germany) for 2 weeks from the start of the test. The experimental results are shown in Table 2 below.

Skin moisturizing effect 1 division Before use After 2 weeks of use After 4 weeks of use Example 1 23.7 42.5 45.9 Example 2 22.5 43.6 46.0 Comparative Example 1 21.9 38.4 40.8 Comparative Example 2 22.4 37.1 39.4 Comparative Example 3 22.5 38.2 40.3 Comparative Example 4 23.1 33.0 35.0

As shown in Table 2, as compared with the cosmetic compositions (Comparative Examples 1 to 3) each containing the active ingredient of the moisturizing cosmetic composition of the present invention as a comparative formulation, the moisturizing cosmetic composition of the present invention (Examples 1 and 2 ) Was superior in moisturizing effect.

Experimental Example 2: Moisturizing effect 2 - Skin barrier improvement effect

25 ℃, 45% relative humidity, and 30 healthy women in the room without air flow. Skin barrier damage was induced by using a 1% aqueous solution of sodium lauryl sulfate (SLS). After applying the cosmetic composition of Examples 1 and 2 and Comparative Examples 1 to 4 to the upper arm of the arm, TEWAMETER TM210 C + K eletronic GmHH, Germany) was used to measure the transdermal water loss. That is, TEWL (Transepidermal Water Loss) value was measured by changing the TEWL value with time, thereby confirming the skin barrier recovery rate. The experimental results are shown in Table 3 below.

Skin moisturizing effect 2 - Skin barrier improvement effect division Skin barrier recovery rate (%) 2D 4D 6D 8D Example 1 48.8 63.6 80.9 94.1 Example 2 49.2 63.8 81.5 96.2 Comparative Example 1 40.1 54.0 64.1 80.1 Comparative Example 2 42.9 56.6 63.4 75.4 Comparative Example 3 43.1 57.7 63.5 76.1 Comparative Example 4 38.1 50.3 59.8 71.3

As shown in Table 3, it can be seen that Examples 1 and 2 containing the cosmetic composition for humidification of the present invention have improved transdermal water loss as compared to Comparative Examples 1 to 4.

Experimental Example  3: Feeling  evaluation

For the cosmetic composition prepared according to Examples 1 and 2 and Comparative Examples 1 to 4, 30 persons to be tested were evaluated for feeling.

After 10 to 20 minutes from the application of the cosmetic composition, the following items were evaluated.

<Evaluation items for feeling>

(i) There is no stickiness.

(ii) Smooth texture.

(iii) The skin is moisturizing.

(iv) The moisturizing effect persists.

The evaluation item was rated as +5 when it was not tacky, smooth, moisturizing, and persistent in moisturizing effect was rated as +5, and it was sticky, had bad texture, had no moisturizing feeling, And a case where an unfavorable state occurs occurs as a zero point. The middle portion is evaluated in four stages in total, and the sum of the power source scores is shown in Table 4 below. Therefore, the higher the score, the higher the rating.

Feeling evaluation division There is no stickiness. Rich sense of smoothness Abundant in moisturizing Moisturizing persistence Example 1 95 95 95 90 Example 2 91 94 98 93 Comparative Example 1 96 82 80 83 Comparative Example 2 88 86 79 85 Comparative Example 3 81 80 85 88 Comparative Example 4 88 66 74 59

As shown in the results of Table 3, the moisturizing cosmetic composition of the present invention (Examples 1 and 2) was superior in feeling to the cosmetic composition of the comparative example. Accordingly, the cosmetic composition of the present invention is not only superior in skin moisturizing effect but also excellent in feeling of use, and thus, is useful as a cosmetic composition.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (4)

A cosmetic composition for skin moisturizing containing an organic acid fermentation broth and a natural polymer component,
The organic acid fermentation broth is obtained by fermenting a mixture of acessobacter, Lactobacillus, or a mixture thereof with a mixture of plum, citron, pomegranate and vinegar,
Wherein the natural polymer component is beta-glucan or natto-gum, and the cosmetic composition for moisturizing the skin. The method according to claim 1,
Wherein the organic acid fermentation broth is contained in an amount of 0.001 to 30.0 wt% based on the total weight of the cosmetic composition. The method according to claim 1,
Wherein the natural polymer component is contained in an amount of 0.00001 to 20.0% by weight based on the total weight of the cosmetic composition. The method according to claim 1,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray Wherein the cosmetic composition for skin moisturizes.