KR102566125B1 - COMPOSITION FOR MOISTURIZING SKIN COMPRISING Potentilla supina L. EXTRACT AS AN ACTIVE INGREDIENT - Google Patents

Abstract

The present invention relates to a composition for moisturizing the skin, which contains an extract of Raccoonia japonica as an active ingredient, which is free from skin irritation and has an excellent skin moisturizing effect. It is provided as a cosmetic composition and does not cause skin irritation when applied to the skin. It does not cause trouble, can be used even in the case of having environmental skin diseases, including ingredients derived from natural substances, and can exhibit excellent skin moisturizing effects.
In addition, provided as a food composition, it may exhibit a skin moisturizing effect by ingestion.

Description

A composition for moisturizing skin containing an extract of sagebrush as an active ingredient

The present invention relates to a composition for moisturizing the skin, comprising an extract of Raccoonia japonica as an active ingredient, and more particularly, to a composition with no skin irritation and excellent skin moisturizing effect.

Recently, as the quality of life is improved due to the extension of average sleep due to the development of medical technology, the desire for a healthy life increases, and at the same time, interest in skin care is also increasing.

As a body part directly exposed to the external environment, the skin not only serves as a protective film to protect important organs of our body, but also controls moisture evaporation and protects the body from external infections. However, no matter how much the skin blocks virus penetration from the outside, stress from the outside, such as excessive ultraviolet rays and a polluted environment, causes skin irritation and eventually leads to skin aging.

Specifically, this skin aging phenomenon is mainly due to the cleavage and low-molecularization of hyaluronic acid, the decrease in the ability of the stratum corneum and the dermal layer to retain moisture due to the sluggish production of collagen in fibroblasts, and the loss of elastine fibrous tissue. It is manifested by a decrease in skin elasticity due to weakness.

In particular, the skin plays an important role in controlling the evaporation of moisture to the outside of the body, which transports about 65 to 70% of the water that the human body has, that is, various physiologically active substances necessary for the human body, and helps the skin to maintain a soft and moist state. do

However, constant physical and chemical stimulation from the outside deteriorates skin function and accelerates skin aging. Skin aging also accompanies the overall deterioration of skin components, resulting in easy cracking of the skin. .

In other words, artificial temperature control of heating and cooling due to changes in the environment or lifestyle, skin stress due to various stresses and environmental pollution generated in social life, frequent face washing due to makeup habits, and natural skin aging due to age increase. Due to various causes such as the decrease in moisture in the stratum corneum, the skin becomes dry, the surface becomes rough, and skin aging occurs such as the skin loses its luster and looks dull. there is.

Accordingly, in the field of cosmetics, research on preventing and improving skin aging by preventing moisture loss in the skin and supplementing the lost skin moisture has been continued for a long time, and related materials are also actively applied to products.

Conventionally, polyhydric alcohols, water-soluble polymers, organic acid salts, etc. have been used as moisturizers contained in cosmetics, and occlusive ingredients that prevent moisture evaporation from the skin surface and absorb moisture while being oily as moisturizers A method of increasing water retention in the stratum corneum using humectants, which are moisturizing ingredients, has been widely used.

Examples of the humectants include materials such as glycerin, propylene glycol, 1,3-butylene glycol, and polyethylene glycol as polyols, and lipid components such as ceramide, essential fatty acids, and lipid complexes are used as the sealing component.

However, when applied to the skin, the humectant has a disadvantage in that it feels sticky or wet due to the inherent viscosity and physical properties of polyol, and the sealing components disturb the structure of the emulsified interface film in the emulsified formulation, thereby impairing stability. There is a problem that is unsuitable for manufacturing a transparent gel-like product.

On the other hand, attempts have been made to improve the moisturizing effect by adding and using a sebum-like component, an NMF component, or a moisturizer such as polyol in cosmetics.

For example, there has been an attempt to use additives such as natural moisturizing factors such as sodium pyrrolidone carboxylate (PCA-Na) and sodium lactate, but these have strong electrolytic properties and impair the emulsification stability of cosmetics. Amino acids, collagen, elastin, etc. are also known to have moisturizing ability, but their moisturizing ability is limited.

On the other hand, recently, attempts have been made to use natural plant extracts as raw materials for cosmetics that enhance skin moisturizing or skin barrier function in order to solve the above problems.

A number of documents have been published in relation to cosmetic compositions for skin moisturizing using plant extracts, and as an example, Korean Patent Publication No. 2009-0076625 discloses a composition for moisturizing skin containing a pine needle extract. In addition, Korean Patent Registration No. 10-1410042 discloses a moisturizing cosmetic composition containing nanovesicles containing flopolis.

However, the moisturizing compositions using the natural extracts also do not guarantee a moisturizing effect of a certain level or more, and there is a problem that can cause skin irritation.

Accordingly, the applicant of the present invention has developed a composition containing a natural extract, P. japonica extract, as a composition capable of solving the above problems, minimizing skin irritation, and having an excellent skin moisturizing effect.

KR 10-2015-0086922 A1

An object of the present invention is to provide a composition for skin moisturizing, which contains an extract of Raccoonia japonica as an active ingredient, and has no skin irritation and excellent skin moisturizing effect.

Another object of the present invention is provided as a cosmetic composition, which does not cause skin irritation when applied to the skin, does not cause skin trouble, and contains ingredients derived from natural substances such as sagebrush extract, even when suffering from environmental skin diseases. It is to provide a composition that can be used and exhibits excellent skin moisturizing effect.

Another object of the present invention is to provide a composition that is provided as a food composition and can exhibit a skin moisturizing effect by ingestion.

In order to achieve the above object, the composition for moisturizing the skin according to an embodiment of the present invention includes an extract of P. japonica as an active ingredient.

The composition has no skin irritation and has an excellent skin moisturizing effect.

The extract is extracted by using any one selected from the group consisting of water, lower alcohols having 1 to 6 carbon atoms, and mixtures thereof as an extraction solvent.

The composition may further include a fermented seaweed extract.

A cosmetic composition for moisturizing the skin according to another embodiment of the present invention includes the composition for moisturizing the skin.

The cosmetic composition may be a formulation selected from the group consisting of skin, serum, lotion, emulsion, cream, essence, lotion, foundation, pack, soap, surfactant-containing cleansing, spray, and powder.

Functional food composition for moisturizing the skin according to another embodiment of the present invention includes the composition for moisturizing the skin.

Hereinafter, the present invention will be described in more detail.

In the human body, the skin is an organ that functions as a protective film that protects the body from the external environment, and plays a role in preventing the loss of biological components, such as water and electrolytes, and preventing harmful substances from entering the body from the outside. do.

Such skin is largely divided into an epidermal layer, a dermal layer, and a subcutaneous fat layer. The epidermal layer is composed of keratinocytes and melanocytes. The outermost keratinocyte layer of the skin directly contacts the external environment, so it requires strong resistance to physical, chemical or substance permeation, and prevents loss of moisture from the body to the outside. At the same time as zooming, it should maintain its flexibility by retaining adequate moisture in itself.

In general, the moisture content of the skin is about 70% in the dermal layer, but decreases toward the epidermal layer, and becomes about 10 to 30% in the keratinocyte layer. The water supplied from the dermal layer moves to the top of the stratum corneum mainly by passive diffusion and is finally discharged to the outside.

This is called Trans Epidermal Water Loss (TEWL), and it is known that sebum and epidermal lipids, which are lipid film components of the keratinocyte layer, maintain this transepidermal water loss at an appropriate level. In addition, it is known that a substance having a hydrophilic water-holding ability called Natural Moisturizing Factor (NMF) exists in the keratinocyte layer and plays an important role in moisturizing the skin.

When the moisture of the normal keratinocyte layer is maintained at about 10 to 30%, the skin is smooth and soft, and the body protective function is normally exhibited. However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and loses the body's protective function, resulting in aging.

For example, in the case of dry skin, the cohesion of keratinocytes is weakened, resulting in a scaling phenomenon in which keratinocytes are peeled off like scales from the skin surface. This is because it is less than normal skin.

In addition, even healthy skin may suffer from moisture deficiency due to harsh external environments, such as wind, cold weather, sunlight, face washing, and shaving, resulting in poor skin condition.

Therefore, it is very important to properly maintain the moisture content of the keratinocyte layer of the skin. It is known that when the moisture content of the stratum corneum is less than 10%, the skin becomes dry and causes skin trouble. The amount of water in the stratum corneum produced by regular keratinization always maintains 10 to 20%.

Currently, an occlusive agent is used to maintain moisture in the skin. The occlusive agent is a hydrophobic substance that promotes water retention by forming a barrier on the skin to prevent water loss.

The most commonly used occlusive agents include petrolatum, lanolin, cocoa butter, mineral oil and silicones. In addition, to make cosmetics, 20 to 50 different types of raw materials are generally used, such as sodium lauryl sulfate, dimethyl sulfoxide, croton oil, and sodium hydroxide. When these raw materials are applied to products, they can cause irritation such as inflammation, itching, and allergies to the skin.

The main feature of the composition of the present invention is that it does not irritate the skin when applied to the skin and exhibits an excellent skin moisturizing effect.

A composition for moisturizing skin according to an embodiment of the present invention includes an extract of P. japonica as an active ingredient.

The Potentilla supina L. is a perennial plant belonging to the family Primrose, and the small leaves (lobules) of the Potentilla supina have deep sawtooth on the edge, and are also called quills because their shape resembles a pitchfork. In Korea, it grows in fields, roadsides, and clearings all over the country. Stem height is about 20 to 50 cm. The lower part grows obliquely to the side and then stands straight. Leaves face each other and are pinnate compound leaves composed of 2 to 4 pairs of small leaves, with long petioles. Small leaves are obovate, elliptical or lanceolate, 0.7 to 2.5 cm long, 0.8 to 1.4 cm wide, with rounded ends and sawtooth edges. Several yellow flowers with a diameter of 6 to 8 mm hang from May to September in the axils at the ends of branches. The fruit is an achene, ripens in September to October, and is characterized by being hairless. Young stems and leaves are edible, and are distributed in temperate regions of the northern hemisphere including Korea.

The extract may be extracted using any one selected from the group consisting of water, lower alcohols having 1 to 6 carbon atoms, and mixtures thereof as an extraction solvent.

Specifically, pulverizing the dried dog urchins; leaching the pulverized product using an organic solvent; drying the sample after leaching; re-leaching the dried sample using an organic solvent; drying the sample after leaching; leaching with water; And including the step of leaching, it is possible to obtain an extract of P.

The method may further include performing fractionation of the extract of P. japonica extract extracted using the organic solvent using an organic solvent.

A method for preparing the extract may be a conventional extraction method in the art, such as an ultrasonic extraction method, a leaching method, and a reflux extraction method. Specifically, it may be an extract obtained by extracting a natural product from which foreign substances are removed by washing and drying with water, alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, or may be an extract extracted by sequentially applying the solvents to a sample.

The reflux extraction method is based on 100 mL of alcohol having 1 to 6 carbon atoms, 10 to 30 g of pulverized natural product, 1 to 3 hours of reflux, and 50 to 100% of alcohol having 1 to 6 carbon atoms. More specifically, based on 100 mL of alcohol having 1 to 6 carbon atoms, 10 to 20 g of pulverized natural product, 1 to 2 hours of reflux, and 70 to 90% of alcohol having 1 to 4 carbon atoms.

The leaching method is by 15 to 30 ℃, for 24 to 72 hours and 50 to 100% of alcohol having 1 to 6 carbon atoms. More specifically, at 20 to 25° C. for 30 to 54 hours and 70 to 80% of an alcohol having 1 to 6 carbon atoms.

The ultrasonic extraction method is by 30 to 50 ℃, 0.5 to 2.5 hours and 50 to 100% of alcohol having 1 to 6 carbon atoms. Specifically, at 40 to 50° C. for 1 to 2.5 hours and 70 to 80% of an alcohol having 1 to 6 carbon atoms.

The extraction solvent may be used in an amount of 2 to 50 times, more specifically, 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for leaching in the extraction solvent for 1 to 72 hours, more specifically 24 to 48 hours.

After extraction, the extract can be fractionated by sequentially applying a fresh fractionation solvent. The fractionation solvent used in the fractionation is at least one selected from the group consisting of water, hexane, butanol, ethylacetic acid, ethyl acetate, methylene chloride, and mixtures thereof, and is preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, a method such as concentration or lyophilization may be additionally used.

The composition may further include a fermented seaweed extract. The composition for moisturizing the skin can be provided as a moisturizing composition with excellent moisturizing effect and a high preference even when it contains only the extract of Raccoonia japonica, but it can exhibit an increased moisturizing effect by using a mixture with a fermented seaweed extract and is a highly desirable cosmetic. It enables provision as a composition or food composition.

In addition, when the seaweed extract is used as it is, the fermented seaweed extract has a problem in that the preference is lowered due to the peculiar odor of seaweed. In order to prevent such a problem, when an active ingredient is extracted from fermented seaweed and mixed with the extract of sagebrush, it is possible to provide a composition with a high preference as well as a high moisturizing effect.

The seaweed extract is specifically, seaweed extract, seaweed extract, capricorn extract, seaweed extract, fusiformis extract, wakame extract, laver extract, kelp extract, Ecklonia cava extract, agar-agar extract, hearing extract, chlorella extract, seaweed extract, semogasari extract, rhubarb It is selected from the group consisting of extracts and mixtures thereof, and preferably may be selected from the group consisting of sea string extract, semogasari extract, and mixtures thereof, but is not limited to the above examples.

Gracilaria verrucosa is a type of red seaweed that belongs to the family Dolphinidae and grows attached to stones and shells in intertidal zones such as Korea, Japan, Sakhalin Island, and Taiwan. In particular, it breeds on pebbles or piles of shallow beaches where river water flows into the sea, and also grows on reefs in the open sea. The body is gathered from a small tray-shaped root and has a cylindrical shape. It is densely branched into a feather shape, and the branches are biased to one side. Branches protruding from the body are cartilaginous, wire-shaped with a diameter of less than 1 mm, blackish purple or dark brown, 1-3 mm in diameter, and grow up to 2-3 m in cylinder. It is also used as a raw material.

The triangle (Gloiopeltis furcate) is a reddish-brown annual seaweed, meaning that the stem is thin in the middle of the furgasari. Samogasari is 5 to 15 cm high, the body is cylindrical, and the branches split a little irregularly, and the body shape varies greatly depending on where it grows. The branches are hollow, the branches are constricted, and the width of the branches reaches 4mm. Semogasari grows profusely throughout winter and spring, matures in early summer, and produces spores.

The released seaweed extract is subjected to a fermentation aging process by putting the washed seaweed in a fermentation vessel and inoculating microorganisms thereon.

The microorganisms are Lactobacillus salivarius, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus rhamnosus, Lactobacillus plantarum, lactobacillus Bacillus helveticus, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus reuteri ( Lactobacillus reuteri), Lactobacillus buchneri, Lactobacillus gasseri, Lactobacillus johonsonii, Lactobacillus kefir, Lactobacillus lactis, Lactococcus lactis), Lactococcus plantarum, Lactococcus raffinolactis, Enterococcus faecalis, Enterococcus faecium, Streptococcus thermophilus thermophilus), Leuconostoc lactis, Leuconostoc mesenteroides, etc. Lactobacillus and Bifidobacterium animals, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium pseudolongum, Bifidobacterium thermophyllum (Bifidobacterium themophilum), Bifidobacterium adolsentis (Bifidobacterium adolescentis), and the like, and more preferably Lactobacillus casei, Lactobacillus rhamnosus (Lactobacillus rhamnosus), bifido It may be selected from the group consisting of Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium breve, and Lactobacillus acidophilus. It is not limited and can be used without limitation.

After inoculating the microorganism, a fermentation aging process is performed at 30 to 35 ° C. for 1 to 2 days. After the fermentation and aging process is completed, the fermented seaweed extract is prepared by the extract preparation process described above.

The composition may include 20 to 40 parts by weight of the fermented hairtail extract and 20 to 40 parts by weight of the fermented Samogasari extract, based on 100 parts by weight of the extract. When mixed and used within the above range, skin irritation does not occur, the moisturizing effect is relatively improved, and skin application properties can be improved.

A cosmetic composition for moisturizing the skin according to another embodiment of the present invention includes the composition of the present invention.

The cosmetic composition of the present invention may be prepared by mixing a dermatologically acceptable carrier in addition to the composition of the present invention.

The dermatologically acceptable carrier may include purified water, oil, wax, fatty acid, fatty alcohol, fatty acid ester, surfactant, moisture absorbent, thickener, antioxidant, viscosity stabilizer, chelating agent, buffer, preservative, lower alcohol, and the like. However, it is not limited thereto, and the type and concentration are various, and include parts that can be changed by those skilled in the art within the scope of the present invention.

In addition, if necessary, functional substances such as whitening agents, moisturizing agents, anti-inflammatory agents, antibacterial agents, antifungal agents, vitamins, sunscreens, antibiotics, anti-acne agents, perfumes, and dyes may be included, which are generally used in the cosmetic field. As may be included in the cosmetic composition according to the present invention. The cosmetic composition of the present invention may contain at least one moisturizing active ingredient exhibiting the same or similar function in addition to the composition of the present invention in order to enhance its functional effect.

Cosmetics prepared from the cosmetic composition of the present invention can be prepared in the form of general emulsified formulations and solubilized formulations. Emulsified cosmetics include nutrition lotion, cream, essence, etc., and solubilized cosmetics include softening lotion.

In addition to the composition of the present invention, the cosmetic may be prepared in the form of an adjuvant that can be applied topically or systemically, which is commonly used in the art, by containing a dermatologically acceptable medium or base.

Suitable cosmetic formulations include, for example, solutions, gels, solid or pasty anhydrous products, emulsions obtained by dispersing an oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), non-ionic forms. It may be provided in the form of a follicular dispersion, cream, toner, lotion, powder, ointment, spray or conceal stick. In addition, it can be prepared in the form of a foam (foam) or the form of an aerosol composition further containing a compressed propellant.

Products to which the cosmetic composition of the present invention can be added include, for example, cosmetics such as astringent lotion, softening lotion, nutrient lotion, various creams, essences, packs, foundations, etc., and cleansing, face wash, soap, treatment or beauty essence etc. Specific formulations include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, essence, nutrient essence, pack, soap, shampoo, cleansing Formulations such as foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder or eye shadow are included.

A food composition according to another embodiment of the present invention includes the composition for moisturizing the skin.

At this time, the amount of the extract in the food or beverage may be added at about 0.01 to 15% by weight of the total food weight, and the health drink composition may be added at a rate of about 0.01 to 10g based on 100 ml, but is not limited thereto .

When the food composition of the present invention is a beverage composition, there is no particular limitation on other ingredients except for containing the extract as an essential ingredient in the indicated ratio, and it may contain various flavoring agents or natural carbohydrates as additional ingredients like conventional beverages. can Examples of the aforementioned natural carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) may be used, but are not limited thereto. The ratio of the natural carbohydrates may generally range from about 1 to about 20 g per 100 ml of the composition of the present invention, but may not be limited thereto.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like may be included, but may not be limited thereto.

In addition, the composition of the present invention may contain natural fruit juice and fruit flesh for producing fruit juice beverages and vegetable beverages, and these components may be used independently or in combination. The ratio of these additives is not critical, but may be selected in the range of about 0.1 to about 20 parts by weight per 100 parts by weight of the composition of the present invention, but is not limited thereto.

A functional food composition according to another embodiment of the present invention may include the composition for moisturizing the skin.

The functional food referred to in the present invention is a food manufactured and processed by extracting, concentrating, refining, mixing, etc., a specific ingredient as a raw material or a specific ingredient contained in a food raw material for the purpose of health supplementation, and includes health supplement food. In addition, it is a food designed and processed to sufficiently exert the biological control functions of food components, such as bio-defense, regulation of biological rhythm, prevention and recovery of disease, etc., with functions related to disease prevention and recovery. make it all inclusive.

The present invention relates to a composition for moisturizing the skin, which contains an extract of Raccoonia japonica as an active ingredient, which is free from skin irritation and has an excellent skin moisturizing effect. It is provided as a cosmetic composition and does not cause skin irritation when applied to the skin. It does not cause trouble, can be used even in the case of having environmental skin diseases, including components derived from natural substances, and can exhibit excellent skin moisturizing effects.

In addition, provided as a food composition, it can exhibit a skin moisturizing effect by ingestion.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

manufacturing example

Manufacture of sagebrush extract

After obtaining only the aerial part of the dog sorrel, it was washed, dried, pulverized to prepare a pulverized product, and freeze-dried. 1 mg of 3 g of lyophilized sagebrush was put into a 15 ml tube, and 10 ml of 70% ethanol was added again. The samples were mixed and extracted for 18 hours in a rounded mixing machine. After extraction, only the supernatant was collected after centrifugation at 3000 rpm for 10 minutes. Ethanol (ethanol) contained in the collected supernatant was evaporated at 40 ℃. And the remaining samples were lyophilized. The extract (EE) used in this experiment was extracted with a yield of 11.5% from the raw material of the dog's cockroach.

Preparation of fermented seaweed extract

After drying the string, it was pulverized using a grinder. Lactobacillus rhamnosus was inoculated into the pulverized sea string and fermented at 25 ° C. for 3 days to prepare fermented sea string. Afterwards, a fermented sea urchin extract (GE) was prepared in the same manner as the method for preparing the extract of P. japonica.

The fermented semogasari extract (FE) was prepared using the same method as the fermented hairpin extract.

Preparation of mixed extract

The extract (EE), the fermented hairtail extract (GE), and the fermented semogasari extract (FE) were mixed and used as shown in Table 1 below.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative Example 2 EE 100 - - 100 100 100 100 100 GE - 100 - 20 30 40 10 50 FE - - 100 20 30 40 10 50

(unit weight parts)

Experimental Example 1

skin irritation test

The irritation relieving effect was confirmed through a human skin patch test on 15 women in their 20s and 30s who had no past history of hypersensitivity to skin irritation and no current skin disease or skin allergy symptoms. Examples and comparative examples were used in which 1.0% by weight of lactic acid, a skin irritant, was added as an irritant.

First, after washing with 70% by weight of ethanol for the participant's test, it was dried. 15 μg of the prepared test substance was dropped into a fin chamber (Finn chamber, 100x10, EPITEST, Finland), and then sealed patched on the inside of the forearm of the test subject. After applying the patch for 24 hours and removing the patch, the test site was marked with a marking pen. After 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, USA) to observe the presence or absence of erythema and edema.

Skin reactions were determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) in Table 2, and the results are shown in Table 3.

sign score Evaluation standard - 0 unresponsive ± 0.5 faint or mild erythema + One Well-circumscribed but mild erythema, edema, and papules ++ 2 Marked erythema, papules and vesicles +++ 3 Severe erythema and blisters, scab formation

[Equation 1]

Mean response rate = (score x number of responses x 100 x 1/2)/[3 (maximum score) x total number of subjects (n)]

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative Example 2 Reactivity (%) 3.11 3.99 3.70 2.51 2.00 2.11 6.75 6.66

According to the above experimental results, it was confirmed that skin irritation did not occur when using the extract of Raccoonalis japonica, and severe skin irritation did not occur even when using the fermented hairpin extract and the fermented semogasari extract. However, in the case of the complex extract, when mixed and used within a specific range, it was confirmed that the best skin irritation relief effect was exhibited when used due to the synergistic effect of the mixed use between the extracts.

Experimental Example 2

Moisturizing effect experiment

Measurement of water holding capacity The water holding capacity was measured by using a Corneometer (Courage + Khazaka, GmbH, Germany) to measure the electrical conductivity of the skin surface. A certain amount (0.03g/16cm ² ) of each cosmetic prepared in Comparative Example was applied to the inside of the lower chest of 15 test subjects, and the moisture content of the skin before application and 2 hours after application was measured. Normal skin without any treatment was used as a control group. was used as The results are shown in Table 4 below.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative Example 2 before application 50 50 50 50 50 50 50 50 2 hours later 75 66 61 83 90 82 52 51

According to the above experimental results, it was confirmed that when using the extract of P. japonica, an excellent skin moisturizing effect was shown even after 2 hours, and when used as a complex extract, an excellent skin moisturizing effect was exhibited within a specific range.

Experimental Example 3

Skin applicability evaluation

Cosmetic creams were prepared using the compositions of Examples and Comparative Examples, and their application properties were evaluated.

3% by weight of each of the compositions of Examples and Comparative Examples, 1% by weight of ascorbic acid-2-magnesium phosphate, 1% by weight of water-soluble collagen (1% aqueous solution), 0.01% by weight of sodium citrate, 0.05% by weight of citric acid, 1,3- A cream was prepared by mixing 3% by weight of butylene glycol and the remaining purified water, and 6 products were provided to 15 women in their 20s and 30s, and then a relative evaluation of skin applicability was requested. Evaluation was requested on a scale of 1 to 5, and the higher the score, the better the product.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Comparative Example 1 Comparative Example 2 skin spreadability 3 2 2 4 5 4 One One

According to the evaluation results in Table 5, the spreadability was evaluated to be moderate in the cream containing only the extract of P. japonica, but when the complex extract was used, it showed excellent spreadability, increasing the possibility of providing a cream with high preference.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also made according to the present invention. falls within the scope of the rights of

Claims (7)

Containing the extract of the aerial part of sagebrush as an active ingredient,
With respect to 100 parts by weight of the extract of the above-ground part of the sagebrush,
20 to 40 parts by weight of fermented seaweed extract and 20 to 40 parts by weight of fermented semogasari extract
A composition for moisturizing the skin. According to claim 1,
The composition has no skin irritation and has an excellent skin moisturizing effect.
A composition for moisturizing the skin. According to claim 1,
The extract is extracted by using any one selected from the group consisting of water, lower alcohols having 1 to 6 carbon atoms, and mixtures thereof as an extraction solvent.
A composition for moisturizing the skin. delete Claims 1 to 3, comprising the composition for moisturizing the skin according to any one of the preceding
A cosmetic composition for moisturizing the skin. According to claim 5,
The cosmetic composition is a formulation selected from the group consisting of skin, serum, lotion, emulsion, cream, essence, lotion, foundation, pack, soap, surfactant-containing cleansing, spray and powder
A cosmetic composition for moisturizing the skin. Claims 1 to 3, comprising the composition for moisturizing the skin according to any one of the preceding
Functional food composition.