KR20150086922A - Composition for relieving skin irritation and moisturizing skin containing natural fermented pine leaf extract - Google Patents

Abstract

The present invention provides a pine needle-off fermentation broth composition having excellent skin moisturizing effect and excellent anion and far-infrared emission effect. The compositions for skin moisturizing according to the present invention include pine needle fermentation broth extract, western horse mackerel extract, rhododendron extract, screen wind extract, eucalyptus leaf extract, collagen extract, oligopeptide-10 and EGF. The skin moisturizing composition according to the present invention is free from skin irritation, has a long-lasting skin moisturizing effect, and has an effect of excellent anion and far-infrared ray extraction efficacy.

Description

Compositions for relieving skin irritation and moisturizing skin containing natural fermented pine leaf extracts -

The present invention relates to a skin moisturizing composition containing a natural fermented pine leaf extract. More particularly, the present invention relates to a composition for skin moisturizing, which comprises a pine needle leaf extract, a horseradish peroxidase extract, a rhubarb extract, a centurion extract, a eucalyptus extract, And epidermal growth factor (EGF) as active ingredients, it is possible to obtain a synergistic effect on skin irritation mitigation and skin moisturizing composition containing natural fermented pine needle extract.

Conventionally, various methods for alleviating skin irritation have been attempted. For example, a method of removing a substance causing skin irritation has been attempted. However, such a study is limited because the functional raw material itself acts as a stimulus source. For example, lactic acid, glycolic acid, salicylic acid, and retinoid are contained in cosmetics for the promotion of skin cell regeneration or anti-wrinkle effect, but they cause skin irritation do. Recently, many studies have been made to neutralize the acidity by adding strong alkali such as NaOH or KOH to the stimulant-inducing functional raw material to alleviate the irritation, but such a prescription has a problem of deteriorating the efficacy of the existing raw materials. Recently, studies have been carried out on inhibition of cell-mediated immunity. Neuropepetides such as calcitonin gene-related peptide (CGRP) and substance P (sunstance P) secreted from neurons, Various cytokines and PGE2 in skin and immune cells, such as kinins such as bradykinin, activated by stress in tissue or plasma, are involved in edema formation and vasodilation, It has been known that it acts as a cause. Neuropeptide antagonists including substance P, bradykinin antagonists and COX inhibitors have been proposed as substances capable of reducing skin irritation and irritation. (Korean Patent Publication No. 2000-0058730), raspberry leaf or fruit (Korean Patent Laid-Open Publication No. 2003-0015654) and a cosmetic composition containing phytosterol (Japanese Patent Application Laid-Open No. 2002-0063987). However, these compositions did not show a clear effect.

On the other hand, skin is an organ which exists in the outermost part of the body and has the function of protecting the human body. It protects the body from moisture and electrolyte loss and protects it from infiltration of foreign matter. Skin is divided into epidermal layer, dermal layer and subcutaneous fat layer. The epidermis is composed of keratinocytes, and the outermost stratum corneum of the epidermis plays a very important role in permeation of the material.

The epidermis is composed of the basal layer, the superficial layer, the granular layer and the stratum corneum. The stratum corneum is composed of keratinocyte and intercellular lipid. The stratum corneum prevents the loss of moisture to the outside, and proper function can be performed by holding proper moisture. The skin barrier located in the stratum corneum is composed of ceramide, fatty acid and cholesterol, which inhibits penetration of foreign substances and prevents the transepidermal water loss (TEWL). It is known that Natural Moisturizing Factor (NMF) is present in the stratum corneum and plays an important role in moisturizing the skin.

The water content of the epidermal layer is about 70% in the basal layer and 30-50% in the granular layer, and 15-30% in the stratum corneum (Journal of Investigative Dermatology, 116, 434442, 2001). That is, the moisture content in the epidermis shows a gradually decreasing skin water gradient, and the skin is subjected to sustained osmotic stress even through this water slope.

In addition, since skin exists in the outermost layer of the human body, it is exposed to air pollution, dry environment, ultraviolet rays, wind, chemicals and the like. This condition exposes the skin to continuous osmotic stress and promotes more dry skin.

In recent years, as the number of people with dry skin increases, the need for moisturizing agents is increasing. Conventionally, when a formulation containing a skin lipid component as a skin moisturizer is used for restoring and strengthening skin barrier, when a moisturizer is developed by applying a natural moisturizing factor or a polyol such as glycerin or butyleneglycol, There are many methods to develop skin moisturizer by promoting differentiation. However, as long as moisture is continuously lost by the external environment, existing moisturizing agents have a great difficulty in maintaining skin moisture for a long time.

The present invention is to provide a cosmetic composition which can alleviate skin irritation and is effective for moisturizing the skin.

In order to solve the above-mentioned problems, the present invention may include the following means.

The cosmetic composition for alleviating skin irritation according to the present invention contains, as an active ingredient, a natural fermented pine needle extract, Sejangsan extract, rhubarb extract, Centella asiatica extract, eucalyptus extract, collagen extract and oligopeptide-10.

The compositions for alleviating skin irritation and moisturizing the skin containing the natural fermented pine leaf extract according to the present invention contain pine needle extract, western medicine extract, rhubarb extract, Centella asiatica extract, eucalyptus leaf extract, collagen extract and oligopeptide-10 as active ingredients .

The pine needle extract, the western medicine extract, the rhubarb extract, the centipede extract, the eucalyptus leaf extract, the collagen extract and the oligopeptide-10 are each contained in an amount of 1 to 8% by weight based on the total weight of the cosmetic composition.

The cosmetic composition is characterized by having formulations of lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.

The present invention can provide a synergistic effect for relieving skin irritation and moisturizing the skin by containing pine needle extract, western medicine extract, rhubarb extract, Centella asiatica extract, eucalyptus leaf extract, collagen extract and oligopeptide-10 as an active ingredient.

Hereinafter, the present invention will be described in detail.

The atopic skin eczema relieving composition of the present invention contains 5-10 wt% of pine leaf extract.

In the composition of the present invention, the pine leaf extract cleans the skin of the skin and cleanses the skin. It has a soothing effect due to its unique sterilizing action and acts to mitigate itching and irritation.

Pine needles, which are the raw materials of pine needles, are rich in terpene phytoncide, which generate anions themselves, and contain a large amount of vitamins and minerals that are beneficial to the human body. In addition, pine needles provide a sole to the final composition to enhance user satisfaction. In the present invention, a pine needle leaf extract, which is a liquid component recovered by pulverizing and filtering the pine needle, is included to form a mixture.

It is well known that Terpenoids ingredients of pine needles prevent skin aging.

In addition to terpenes, the pine needles, especially the α-Finene ingredients contained in pine needles, become isomerized into Campone, which promotes the process of reducing the keratinization and keratinization of living tissue.

As a process for obtaining the pine leaf extract, first, the collected pine leaves are immersed in sufficient water for more than one day to prevent the pine root. Remove the pine needles and dry them for a day, then crush the pine needles with a pounding machine, for example, a universal plow.

The pulverization is preferably carried out by a primary pulverization and a secondary pulverization. In the primary pulverization, the pulverization is performed at 8-10 mm or less, and in the secondary pulverization, the pulverization is performed at 3-4 mm or less. It is preferable to sufficiently shake after each grinding. Next, the crushed pine needles are wrapped with a mesh, liquid separation is performed with a hydraulic compression press, and the mixture is separated into a supernatant and a slurry when kept in a standing state for 24 hours or longer. When the supernatant is separated by filtration of the slurry, the pine leaf extract can be obtained. In addition to this method, various extraction methods conventionally used in the art can be used. Preferably, water, about 1 to 15 times, preferably about 5 to 10 times water, lower alcohol or the like In a mixed solvent having a mixing ratio of from about 1: 0.1 to 1: 10, preferably from 1: 1 to 1: 3, of from about 1 hour to about 2 days at an extraction temperature of from 20 to 100 DEG C, Preferably about 2 hours to about 1 day, by filtration, concentration under reduced pressure, or drying to obtain an extract of pine needles extract, using a method such as cold-water extraction, hot water extraction, ultrasonic extraction or reflux cooling extraction.

Mix the castor oil extract and alkaline water with the pine needle extract powder obtained in the first step.

The addition amount is preferably 95 to 98% by weight of pine needle extract, 1-2.5% by weight of castor oil, and 1-2.5% by weight of alkaline water, preferably anion-generating alkaline ionized water. After mixing, they are mixed with a homogenizer, and it is preferable to mix them at 2500 rpm for about 15 minutes.

The final pine needle extract is fermented and aged, and fermented at 24-25 ° C for about 60-80 hours to produce a pine needle extract.

In addition, the atopic skin eczema relieving composition of the present invention may further contain 5 to 10% by weight of an anion generating alkaline ionized water derived from a plant ingredient. When the amount of the anion generating alkaline ionized water is less than 5% by weight, the effect of releasing the anion may be lowered. When the amount of the anion generating alkaline ionized water exceeds 10% by weight, the content of the other components of the atopic skin eczema relieving composition of the present invention is relatively .

The western Crataegus oxyacantha used in the present invention is also called a pea may flower in May belonging to a rose. It is said that the lightning storm is effective for storm storm.

Vasodilatory action, coercive action, corpuscle action or digestive action, cardiac action, branch action, analgesic action, sebum action, skin regeneration effect, etc. Partially dried fruit is preferably used, and the fruit contains uric acid, oleanolic acid, rutin, etc., seeds include amigallin, hyperperin, and fat oil, and bark contains asuculine.

The rhubarb origin used in the present invention is native to the present-day rhubarb which originated in the country of Seochang and Cheonghae in China and was grown in a natural state because the seed was sown on the fields and fields. In addition, you can see the growing up with a mule in a wetland or a stream in a mountain valley, or it grows in a field, a field, and a roadside which were cultivated in the past.

It has thick yellow roots. The height of the main stem which grows straight is 1m, the stem is hollow, the leaf shape is wide egg-shaped circle, and there are wave-like or wavy wrinkles on the leaves. The flower is bloomed around July to August, and the fruit is triangle-shaped with a capillary, has a wing, is removed from the roots of a root of six to seven years, It is medicinal.

In one room, it is used as an anti-inflammatory laxative (lowering agent), and it is used in combination with various prescriptions. When a small amount is consumed, it exhibits a stomach effect (stomach effect), and in a large amount, it is a laxative It is good for Constipation constipation and indigestion, It is good for constipation and bad digestion, It is also used for burning in the case of medicinal medicine. It is effective for the prevention of eosinophilia and menstruation because it does not have poison and it does not poison. It is good for passing the feces and cooling the heat, and it can be used for menstrual disorders, hematuria, acute peritonitis, Arthritis, fever, fever with fever, different symptoms, swelling, and burns.

In addition, the atopic skin eczema relieving composition of the present invention contains 45% to 50% by weight of Centella asiatica. In the composition of the present invention, the centipede centilla extract has antimicrobial activity and antioxidative effect, and also has an excellent moisturizing action and acts to alleviate itching and irritation of the skin.

The Centella asiaticus extract of the present invention has been used for a long time as a civil remedy, and it is said that it has an excellent effect on wound healing, and ancient Indians are said to have eaten two sheets of leaflings every day when their skin is rough. In addition, Centella asiatica extract is used as a therapeutic agent which is very effective for surgical scratches and 2-3 degree burns. In the case of trauma, prescribe ointment containing 0.5-1.0% of asiaticoside to the wound caused by the skin disease, or 2% of the Asian ticoside is recommended dose and it is treated with one or two times a day.

Centella asiatica is the scientific name of the plant, which has been shown to promote the synthesis of collagen.

The results of a rat experiment revealed that the centilla extract showed a wound healing effect of collagen and fibronectin in the dermis.

In terms of skin care, it plays an important role in helping the regeneration of aged skin.

Especially, it is known that the product contains the centilla extract, which plays a role in coordinating itching and irritation.

It is a native plant of Korea, a plant commonly found in wet streams, etc., and can be directly grown and used. It can also be obtained from a flower garden or the like.

In particular, in the present invention, it is preferable to use a wisteria fermentation extract, and a method for obtaining a wisteria fermentation extract is as follows. First, the leaflings and roots are washed and pulverized. Next, the pulverized material is injected into a ceramic material-made reactor in which air is permeated and the liquid component is not permeated, and then fermented by allowing it to stand at 25-28 캜 for 72 hours. By this fermentation, the pulverized seedlings are subjected to solid / liquid separation. The above-mentioned high / liquid separated cocoon fermented product is filtered by a filter to obtain a fungal flower fermented extract

Collagen is a type of fibrous protein that acts as a link between cells and cells.

It is very important as a component of the dermis layer in the skin which functions as an adhesive of calcium constituting the bone and wrinkles, especially in the membrane covering the organs, joint cartilage, eye cornea, bones and skin.

Collagen is contained in many foods such as fish, chicken wings, meat tendons. They are broken down into amino acids in the intestine and then synthesized into collagen, which is then supplied to the organs, epidermis, muscles and joints of the whole body.

However, if you are over 20 years old, the amount produced in the body will decrease sharply and the metabolism of the whole body will become dull. The lack of glue causes the calcium to escape from the bone and the cartilage to become insufficiently hydrated.

Wrinkles and dullness are also caused by lack of collagen, which accounts for 70% of the skin's dermis.

The third-generation "collagen" extracted from carrots rejuvenates the skin and helps prevent aging and wrinkling of the skin. It deeply penetrates into the dermis layer and plays an important role in the elasticity, luster, moisturization and wound healing process of the skin.

Membrane [H.E.S] membrance regenerates cells that produce collagen.

The dermis in the skin consists of collagen and elastin fibers, which provide elasticity and elasticity to the skin.

The wrinkles and skin sagging is because collagen fibers have lost their elasticity.

P.M.B [Advanced moisturizer] It is a shoe-per serum that protects skin from external damage such as drying and prevents water from getting out of the inside of skin.

It alleviates the side effects caused by using cosmetics and troubles caused by skin.

"It gives you the power to constantly moisturize and keep your skin moisturized.

Oligopeptide-10

The present invention not only alleviates skin irritation by containing pine needle extract, Western hawthorn extract, rhubarb extract, Centella asiatica extract, eucalyptus leaf extract, collagen extract, oligopeptide-10 and EGF as an active ingredient, A cosmetic composition having an effect on skin moisturization. Each of the extracts according to the present invention can be prepared as follows.

Pine needle, rhubarb, cinnabar, eucalyptus, collagen, oligopeptide-10, and epithelial cell activator (EGF) are washed with water and then dried in the shade. Each of the dried plants is placed in an extraction container and extracted at a suitable temperature for a certain period of time in an appropriate amount of extraction solvent. The extract may be filtered and concentrated using a vacuum rotary evaporator or the like.

The extraction solvent may be a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably ethanol.

The amount of the extraction solvent is 2 to 10 times the dry weight of the herbal medicine.

The extraction method may be an extraction method such as hot water extraction, reflux cooling extraction, ultrasonic extraction, etc., and may be extracted once to five times, preferably as an extraction method of hot water extraction.

The immersion temperature is preferably 30 to 70 ° C, more preferably 40 to 60 ° C, and most preferably 50 ° C or so, which is most effective in preventing decay, discoloration and detachment.

The immersion time is 5 to 20 hours, preferably 10 hours.

After obtaining the extract, the solid content is removed using a filter paper or the like, the suspension is centrifuged, and the supernatant can be filtrated under reduced pressure.

The filtered extract can be concentrated under reduced pressure at 20 to 100 ° C, preferably 50 to 70 ° C with a vacuum rotary condenser.

In the present invention, the pine needle extract, the horseradish extract, the rhizome extract, the screen wind extract, the eucalyptus leaf extract, the collagen extract, the oligopeptide-10 and the epithelial cell activity factor (EGF) Is preferable.

The components contained in the cosmetic composition of the present invention include, in addition to the pine needle extract, pine needle extract, rhododendron extract, screen extract, eucalyptus leaf extract, collagen extract, oligopeptide-10 and EGF, And may contain conventional auxiliary agents such as, for example, antioxidants, stabilizers, solubilizers, pigments and flavoring agents, and carriers.

The cosmetic composition for skin whitening according to the present invention can be used for the purpose of the present invention in addition to pine needle extract, Other ingredients which can increase skin moisturizing effect and skin irritation mitigating effect are preferably contained within the range not impairing the effect. In addition, any of the formulations conventionally manufactured in the art can be prepared, and examples include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powders A foundation, an emulsion foundation, a wax foundation and a spray, but the present invention is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, a cream or a gel, an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, Can be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, alundum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan aliphatic esters.

When the formulation of the present invention is an interfacial active agent-containing cleansing, it is preferable to use, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethiotate, imidazolinium derivative, methyltaurate, sarcosinate, Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable europaeolin derivatives, or ethoxylated glycerol fatty acid esters.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. It is to be understood, however, that the invention is not limited to the disclosed embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. It will be obvious to the person.

[Manufacturing Example 1] Production of Eucalyptus leaf extract, collagen extract, oligopeptide-10, and epithelial cell activator (EGF) D from pine needle fermentation broth, Western hawthorn extract,

50 g of pine needle, western hornbill, rhubarb, screen, eucalyptus leaf, collagen, oligopeptide-10, and epithelial cell activator (EGF) were immersed in 200 ml of 100% by weight ethanol and warmed in a water bath at 50 캜 for 10 hours. The obtained extract was cooled to room temperature and filtered with filter paper to obtain a filtrate. Ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10 wt% ethanol to prepare an extract.

[Preparation Example 2] Preparation of Example

Examples and Comparative Examples were prepared according to Table 1 according to Table 1, which is a pine needle extract, a horseradish extract, a rhubarb extract, a screen wind extract, a eucalyptus leaf extract, a collagen extract, an oligopeptide-10 and an epithelial cell activation factor (EGF).

[Experimental Example 1] Skin irritation mitigation test

Twenty women in their 20s and 30s who have never been hypersensitive to skin irritation due to a history of skin irritation or skin allergic symptoms have been tested for skin irritation by human skin patch test. Examples and Comparative Examples 1 to 6 in which 5.0 wt% of lactic acid, which is a skin irritant, was added as a stimulating agent were used. First, the test site was wiped with 70 wt% ethanol and then dried. The prepared test substance was dripped into a Finn chamber (Finx chamber, 100 × 10, EPITEST, Finland) at 15 μg each, and the test piece was hermetically sealed to the inside of the forearm. After 24 hours of exposure, the patches were removed and marked with the marking pen. After 1 hour and 24 hours after the labeling, the test site was observed using a magnifying glass (8MC-150, DAZOR, USA) to observe erythema and edema. Skin reactions were judged according to the regulations of the International Society for Contact Dermatology (ICDRG, InternationalContact Dermatitis Research Group), and the results are shown in Table 2.

* Criteria and scoring criteria for skin reactions

Symbol score criterion

- 0 No reaction

± 0.5 Faint or mild erythema

+ 1 Clear border with weak erythema, edema and palsy

++ 2 Severe erythema, papules and small follicles

+++ 3 Severe erythema and alveoli, scab formation

[Equation 1]

Mean response rate = (score x number of reactions x 100 x 1/2) / [3 (maximum score) x total subjects (n)]

As can be seen from Table 2, the pine needle extract of the present invention, Western hawthorn extract, rhododendron extract, screen wind extract, eucalyptus leaf extract, collagen extract, oligopeptide-10 and EGF It was found that the skin irritation mitigation effect induced by lactic acid was superior to those of Comparative Examples 1 to 6, because the skin reactivity of the extract, which is an extract, was the lowest at 2.70%.

[Experimental Example 2] Moisturizing effect

In order to evaluate the moisturizing power of each of the cosmetic preparations prepared in the above Examples and Comparative Examples 1 to 7, the water retention capacity and the moisture evaporation amount of the skin to which each cosmetic was applied were measured as follows.

Measurement of water retention capacity The electrical conductivity of the skin surface was measured using a Corneometer (Courage + Khazaka, GmbH, Germany). The measurement temperature was 22 ° C., 45% relative humidity, Each cosmetic preparation prepared in Comparative Examples 1 to 7 was applied to a predetermined amount (0.03 g / 16 cm 2) of 30 testees under test, and the moisture content of the skin was measured before application, 1 hour after application and 2 hours after application, Were used as a control. The results are shown in Table 3 below.

As can be seen from the above Table 3, it was confirmed that the skin moisturizing effect of the cosmetic according to the example of the present invention is superior to the comparative examples.

Claims (4)

Skin irritation mitigation comprising a natural fermented pine leaf extract containing pine needle extract, western hornbill extract, rhubarb extract, Centella asiatica extract, eucalyptus leaf extract, collagen extract, oligopeptide-10 extract and EGF as active ingredients / RTI > A cosmetic composition for moisturizing skin, comprising as an active ingredient, pine needle extract, western hornbill extract, rhubarb extract, Centella asiatica extract, eucalyptus leaf extract, collagen extract, oligopeptide-10 extract and EGF. 3. The method according to claim 1 or 2,
The pine needle extract is 70 to 80% by weight, and the extract of Echinochloa crus-galli, Rhodiola extract, Centella asiatica, Centella asiatica extract, Eucalyptus leaf extract, Collagen extract, Oligopeptide-10 extract and EGF To 8% by weight of the cosmetic composition. The cosmetic composition according to claim 3, wherein the cosmetic composition has a formulation of a lotion, essence, lotion, cream, pack, gel, ointment, patch, or spray.