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ES2061446T3 - Procedimiento para la produccion de productos proteinicos en aspergillus oryzae y un promotor para empleo en el mismo. - Google Patents

Procedimiento para la produccion de productos proteinicos en aspergillus oryzae y un promotor para empleo en el mismo.

Info

Publication number
ES2061446T3
ES2061446T3 ES87103806T ES87103806T ES2061446T3 ES 2061446 T3 ES2061446 T3 ES 2061446T3 ES 87103806 T ES87103806 T ES 87103806T ES 87103806 T ES87103806 T ES 87103806T ES 2061446 T3 ES2061446 T3 ES 2061446T3
Authority
ES
Spain
Prior art keywords
promoter
aspergillus oryzae
procedure
production
sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87103806T
Other languages
English (en)
Other versions
ES2061446T5 (es
Inventor
Esper Boel
Tove Christensen
Helle Fabricius Woldike
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8102403&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2061446(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2061446T3 publication Critical patent/ES2061446T3/es
Publication of ES2061446T5 publication Critical patent/ES2061446T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242Fungal source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6481Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

SE DESCRIBE UN PROCEDIMIENTO PARA LA EXPRESION DE UN PRODUCTO PROTEINICO EN ASPERGILLUS ORYZAE. EL PROCEDIMIENTO COMPREDE TRANSFORMAR ASPERGILLUS ORYZAE CON UN SISTEMA VECTOR QUE COMPRENDE SECUENCIAS DE ADN QUE CODIFICAN FUNCIONES QUE FACILITAN LA EXPRESION GENETICA, UN MARCADOR ADECUADO PARA LA SELECCION DE TRANSFORMANTES, Y UNA SECUENCIA DE ADN QUE CODIFICA ELPRODUCTO PROTEINICO DESEADO. EL PROCEDIMIENTO PREMITE LA PRODUCCION INDUSTRIAL DE MUCHOS Y DIFERENTES POLIPEPTIDOA Y PROTEINAS EN A. ORYZAE. EJEMPLOS DE TALES PRODUCTOS SON LA QUIMOSINA O LA PROQUIMOSINA Y OTROS CUAJOS, PROTEASAS, LIPASAS Y AMILASAS. TAMBIEN SE DESCRIBE UN EFICAZ AGENTE FAVORECEDOR PARA LA EXPRESION DE UNA PROTEINA EN ESPERGILLUS. UN AGENTE FAVORECEDOR PREFERIDO ES EL AGENTE FAVORECEDOR TAKA-AMILASA O PARTES FUNCIONALES DEL MISMO.
ES87103806T 1986-03-17 1987-03-16 Procedimiento para la produccion de productos proteinicos en aspergillus oryzae y un promotor para empleo en el mismo. Expired - Lifetime ES2061446T5 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK122686A DK122686D0 (da) 1986-03-17 1986-03-17 Fremstilling af proteiner

Publications (2)

Publication Number Publication Date
ES2061446T3 true ES2061446T3 (es) 1994-12-16
ES2061446T5 ES2061446T5 (es) 2003-04-01

Family

ID=8102403

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87103806T Expired - Lifetime ES2061446T5 (es) 1986-03-17 1987-03-16 Procedimiento para la produccion de productos proteinicos en aspergillus oryzae y un promotor para empleo en el mismo.

Country Status (9)

Country Link
EP (3) EP0489718B1 (es)
JP (4) JPH0665316B2 (es)
AT (2) ATE282093T1 (es)
CA (1) CA1341593C (es)
DE (2) DE3752379T2 (es)
DK (1) DK122686D0 (es)
ES (1) ES2061446T5 (es)
FI (2) FI108147B (es)
IE (2) IE940343L (es)

Families Citing this family (996)

* Cited by examiner, † Cited by third party
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JPH10276787A (ja) 1998-10-20
JPH0665316B2 (ja) 1994-08-24
DE3788524T3 (de) 2003-04-24
ES2061446T5 (es) 2003-04-01
EP0238023A3 (en) 1989-02-22
FI871144A0 (fi) 1987-03-16
EP0489718B1 (en) 2004-11-10
FI108147B (fi) 2001-11-30
EP1502952A2 (en) 2005-02-02
EP1502952A3 (en) 2005-05-11
JPS62272988A (ja) 1987-11-27
FI871144A (fi) 1987-09-18
IE870682L (en) 1987-09-17
IE63169B1 (en) 1995-03-22
DE3788524D1 (de) 1994-02-03
FI20011797A (fi) 2001-09-12
EP0489718A1 (en) 1992-06-10
JP3005618B2 (ja) 2000-01-31
JP2003153696A (ja) 2003-05-27
JPH0751067A (ja) 1995-02-28
DE3788524T2 (de) 1994-05-11
IE940343L (en) 1987-09-17
DE3752379D1 (de) 2004-12-16
EP0238023A2 (en) 1987-09-23
CA1341593C (en) 2009-04-21
EP0238023B1 (en) 1993-12-22
DE3752379T2 (de) 2005-10-20
ATE282093T1 (de) 2004-11-15
JP3903165B2 (ja) 2007-04-11
FI112376B (fi) 2003-11-28
ATE98993T1 (de) 1994-01-15
DK122686D0 (da) 1986-03-17
EP0238023B2 (en) 2002-10-02

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