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DK84288D0 - Fusionsproteiner og oprensning deraf - Google Patents

Fusionsproteiner og oprensning deraf

Info

Publication number
DK84288D0
DK84288D0 DK084288A DK84288A DK84288D0 DK 84288 D0 DK84288 D0 DK 84288D0 DK 084288 A DK084288 A DK 084288A DK 84288 A DK84288 A DK 84288A DK 84288 D0 DK84288 D0 DK 84288D0
Authority
DK
Denmark
Prior art keywords
purification
fusion proteins
affinity chromatography
metal chelate
chelate affinity
Prior art date
Application number
DK084288A
Other languages
English (en)
Other versions
DK173951B1 (da
DK84288A (da
Inventor
Heinz Doebeli
Bernhard Eggimann
Erich Hochuli
Dietrich Stueber
Reiner Gentz
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of DK84288D0 publication Critical patent/DK84288D0/da
Publication of DK84288A publication Critical patent/DK84288A/da
Application granted granted Critical
Publication of DK173951B1 publication Critical patent/DK173951B1/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
DK198800842A 1987-03-10 1988-02-18 Fusionsproteiner, gener der koder herfor, ekspressionsvektorer, transformeret bakterie, fremgangsmåde til oprensning af fusionsproteiner, fremgangsmåde til oprensning af et biologisk aktivt polypeptid eller protein, vacciner indeholdende fusionsprotei... DK173951B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH89587 1987-03-10
CH89587 1987-03-10

Publications (3)

Publication Number Publication Date
DK84288D0 true DK84288D0 (da) 1988-02-18
DK84288A DK84288A (da) 1988-09-11
DK173951B1 DK173951B1 (da) 2002-03-04

Family

ID=4197678

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198800842A DK173951B1 (da) 1987-03-10 1988-02-18 Fusionsproteiner, gener der koder herfor, ekspressionsvektorer, transformeret bakterie, fremgangsmåde til oprensning af fusionsproteiner, fremgangsmåde til oprensning af et biologisk aktivt polypeptid eller protein, vacciner indeholdende fusionsprotei...

Country Status (11)

Country Link
US (2) US5284933A (da)
EP (1) EP0282042B1 (da)
JP (1) JP2686090B2 (da)
AT (1) ATE106897T1 (da)
AU (1) AU609783B2 (da)
CA (1) CA1340522C (da)
DE (1) DE3889949D1 (da)
DK (1) DK173951B1 (da)
IE (1) IE63991B1 (da)
NZ (1) NZ223735A (da)
ZA (1) ZA881534B (da)

Families Citing this family (123)

* Cited by examiner, † Cited by third party
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US5310663A (en) 1994-05-10
ATE106897T1 (de) 1994-06-15
JP2686090B2 (ja) 1997-12-08
AU1270988A (en) 1988-09-15
DK173951B1 (da) 2002-03-04
DK84288A (da) 1988-09-11
DE3889949D1 (de) 1994-07-14
US5284933A (en) 1994-02-08
AU609783B2 (en) 1991-05-09
IE880685L (en) 1988-09-10
EP0282042A2 (de) 1988-09-14
NZ223735A (en) 1990-10-26
CA1340522C (en) 1999-05-04
JPS63251095A (ja) 1988-10-18
EP0282042B1 (de) 1994-06-08
IE63991B1 (en) 1995-06-28
EP0282042A3 (de) 1991-09-11
ZA881534B (en) 1988-09-12

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