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DE602004025192D1 - Rekombinante Carboxypeptidase B und ihre Aufreinigung - Google Patents

Rekombinante Carboxypeptidase B und ihre Aufreinigung

Info

Publication number
DE602004025192D1
DE602004025192D1 DE602004025192T DE602004025192T DE602004025192D1 DE 602004025192 D1 DE602004025192 D1 DE 602004025192D1 DE 602004025192 T DE602004025192 T DE 602004025192T DE 602004025192 T DE602004025192 T DE 602004025192T DE 602004025192 D1 DE602004025192 D1 DE 602004025192D1
Authority
DE
Germany
Prior art keywords
carboxypeptidase
purification
zymogen
activated
recombinant carboxypeptidase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602004025192T
Other languages
English (en)
Inventor
Stephan Dr Glaser
Frank Dr Geipel
Thomas Dr Kirschbaum
Bernhard Dr Rexer
Johann-Peter Dr Thalhofer
Rainer Dr Mueller
Claudia Giessel
Hellmut Eckstein
Elvira Wolf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Publication of DE602004025192D1 publication Critical patent/DE602004025192D1/de
Anticipated expiration legal-status Critical
Active legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE602004025192T 2003-12-05 2004-12-02 Rekombinante Carboxypeptidase B und ihre Aufreinigung Active DE602004025192D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03028101 2003-12-05

Publications (1)

Publication Number Publication Date
DE602004025192D1 true DE602004025192D1 (de) 2010-03-11

Family

ID=34626388

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004025192T Active DE602004025192D1 (de) 2003-12-05 2004-12-02 Rekombinante Carboxypeptidase B und ihre Aufreinigung

Country Status (7)

Country Link
US (1) US20050142633A1 (de)
JP (2) JP4411192B2 (de)
CN (1) CN1311074C (de)
AT (1) ATE455849T1 (de)
CA (1) CA2486195C (de)
DE (1) DE602004025192D1 (de)
ES (1) ES2337684T3 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101016540B (zh) * 2006-02-10 2010-04-07 台湾尖端先进生技医药股份有限公司 C-端含有组氨酸标记的hoxb4重组蛋白质的制造方法及用途
EP2036978A1 (de) * 2007-09-14 2009-03-18 URSAPHARM Arzneimittel GmbH & Co. KG Rekombinante Herstellung ausgewählter Bromelain-Fraktionen
JP2011529459A (ja) * 2008-07-31 2011-12-08 ウルサファルム アルツナイミッテル ゲゼルシャフト ミット ベシュレンクテル ハフツング ブロメラインインヒビター及びブロメラインインヒビター前駆体の組換え体調製物
US8759054B2 (en) * 2009-08-04 2014-06-24 Council Of Scientific & Industrial Research DNA loaded supported gold nanoparticles, process for the preparation and use thereof
CN102286502A (zh) * 2011-07-28 2011-12-21 甘李药业有限公司 重组羧肽酶b的制备方法
CN109136208A (zh) * 2018-09-07 2019-01-04 江南大学 一种羧肽酶b分泌表达的方法
CN109609490A (zh) * 2018-12-30 2019-04-12 江苏鸣生物股份有限公司 一种活化谷氨酰胺转氨酶酶原的方法

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ199722A (en) * 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
US4870008A (en) * 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4808537A (en) * 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US5166329A (en) * 1985-10-25 1992-11-24 Phillips Petroleum Company DNA encoding the alcohol oxidase 2 gene of yeast of the genus Pichia
US4818700A (en) * 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US5032516A (en) * 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
US5135868A (en) * 1985-10-25 1992-08-04 Phillips Petroleum Company Cultures of yeast of the genus Pichia altered by site selective genomic modification
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
US4812405A (en) * 1986-02-18 1989-03-14 Phillips Petroleum Company Double auxotrophic mutants of Pichia pastoris and methods for preparation
US4857467A (en) * 1986-07-23 1989-08-15 Phillips Petroleum Company Carbon and energy source markers for transformation of strains of the genes Pichia
US5002876A (en) * 1986-09-22 1991-03-26 Phillips Petroleum Company Yeast production of human tumor necrosis factor
ZA877505B (en) * 1986-10-14 1989-05-30 Lilly Co Eli Process for transforming a human insulin precursor to human insulin
US4683293A (en) * 1986-10-20 1987-07-28 Phillips Petroleum Company Purification of pichia produced lipophilic proteins
CA1340522C (en) * 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
US4929555A (en) * 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
US5004688A (en) * 1988-04-15 1991-04-02 Phillips Petroleum Company Purification of hepatitis proteins
US5122465A (en) * 1989-06-12 1992-06-16 Phillips Petroleum Company Strains of pichia pastoris created by interlocus recombination
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
AU1178495A (en) * 1993-11-16 1995-06-06 Eli Lilly And Company Dna sequences encoding porcine pancreatic carboxypeptidase b
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
IL116696A (en) * 1995-01-25 1999-08-17 Bio Technology General Corp Production of enzymatically active recombinant carboxypeptidase b
DE19915938A1 (de) * 1999-04-09 2000-10-19 Aventis Pharma Gmbh Herstellung von pankreatischer Procarboxypeptidase B, Isoformen und Muteinen davon und ihre Verwendung
WO2001051624A2 (en) * 2000-01-12 2001-07-19 Eli Lilly And Company Carboxypeptidase b free of animal products and contaminating enyzme activity

Also Published As

Publication number Publication date
JP4411192B2 (ja) 2010-02-10
CN1624122A (zh) 2005-06-08
JP2005168501A (ja) 2005-06-30
CA2486195A1 (en) 2005-06-05
CN1311074C (zh) 2007-04-18
US20050142633A1 (en) 2005-06-30
ES2337684T3 (es) 2010-04-28
CA2486195C (en) 2012-03-06
JP2010046074A (ja) 2010-03-04
JP4964284B2 (ja) 2012-06-27
ATE455849T1 (de) 2010-02-15

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Legal Events

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8364 No opposition during term of opposition