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DK0535005T3 - Forbedret fremgangsmåde og apparat til indføring af biologiske substanser i levende celler - Google Patents

Forbedret fremgangsmåde og apparat til indføring af biologiske substanser i levende celler

Info

Publication number
DK0535005T3
DK0535005T3 DK91909768.3T DK91909768T DK0535005T3 DK 0535005 T3 DK0535005 T3 DK 0535005T3 DK 91909768 T DK91909768 T DK 91909768T DK 0535005 T3 DK0535005 T3 DK 0535005T3
Authority
DK
Denmark
Prior art keywords
improved method
living cells
biological substances
introducing biological
introducing
Prior art date
Application number
DK91909768.3T
Other languages
English (en)
Inventor
Stephen A Johnston
John C Sanford
Ronald F Bruner
Vit Michael J De
Original Assignee
Du Pont
Cornell Res Foundation Inc
Univ Duke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Cornell Res Foundation Inc, Univ Duke filed Critical Du Pont
Application granted granted Critical
Publication of DK0535005T3 publication Critical patent/DK0535005T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mechanical Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK91909768.3T 1990-05-29 1991-04-29 Forbedret fremgangsmåde og apparat til indføring af biologiske substanser i levende celler DK0535005T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/529,989 US5204253A (en) 1990-05-29 1990-05-29 Method and apparatus for introducing biological substances into living cells

Publications (1)

Publication Number Publication Date
DK0535005T3 true DK0535005T3 (da) 1994-09-26

Family

ID=24112008

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91909768.3T DK0535005T3 (da) 1990-05-29 1991-04-29 Forbedret fremgangsmåde og apparat til indføring af biologiske substanser i levende celler

Country Status (13)

Country Link
US (1) US5204253A (da)
EP (1) EP0535005B1 (da)
JP (1) JP2517813B2 (da)
KR (1) KR960008270B1 (da)
AR (1) AR243603A1 (da)
AT (1) ATE110415T1 (da)
AU (1) AU7878891A (da)
CA (1) CA2083816C (da)
DE (1) DE69103631T2 (da)
DK (1) DK0535005T3 (da)
ES (1) ES2059140T3 (da)
HU (1) HU216738B (da)
WO (1) WO1991018991A1 (da)

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HUT67533A (en) 1995-04-28
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