CN113174319A - Culture medium sampler that punches - Google Patents
Culture medium sampler that punches Download PDFInfo
- Publication number
- CN113174319A CN113174319A CN202110466560.6A CN202110466560A CN113174319A CN 113174319 A CN113174319 A CN 113174319A CN 202110466560 A CN202110466560 A CN 202110466560A CN 113174319 A CN113174319 A CN 113174319A
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- Prior art keywords
- punching
- punch
- head
- inner tube
- sampler
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- 239000001963 growth medium Substances 0.000 title claims abstract description 21
- 238000004080 punching Methods 0.000 claims abstract description 63
- 238000005070 sampling Methods 0.000 claims abstract description 30
- 239000000463 material Substances 0.000 claims abstract description 8
- 238000005520 cutting process Methods 0.000 claims description 8
- 238000005192 partition Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 abstract description 8
- 239000007787 solid Substances 0.000 abstract description 6
- 229910001069 Ti alloy Inorganic materials 0.000 abstract description 4
- 239000002609 medium Substances 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 239000010935 stainless steel Substances 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract description 3
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 3
- 229920001817 Agar Polymers 0.000 abstract description 2
- 239000008272 agar Substances 0.000 abstract description 2
- 229910001220 stainless steel Inorganic materials 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 abstract 1
- 230000008878 coupling Effects 0.000 abstract 1
- 238000010168 coupling process Methods 0.000 abstract 1
- 238000005859 coupling reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 210000003484 anatomy Anatomy 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 238000002224 dissection Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 229910000838 Al alloy Inorganic materials 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- PQLXHQMOHUQAKB-UHFFFAOYSA-N miltefosine Chemical compound CCCCCCCCCCCCCCCCOP([O-])(=O)OCC[N+](C)(C)C PQLXHQMOHUQAKB-UHFFFAOYSA-N 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000009928 pasteurization Methods 0.000 description 1
- 229910001256 stainless steel alloy Inorganic materials 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a culture medium sampler that punches, belong to microorganism experimental facilities technical field, structurally by the outer tube, the inner tube, reset spring, clear away the button and punch the sampling head and connect and constitute, carry out sampling operation in solid medium and sample through the removable head of punching with outer union coupling, make through the inner tube after having got the appearance and punch remaining agar culture medium and sample release in the head, punch the sample requirement according to the difference, punch the head and punch through trading different apertures, reset spring in the inner tube is under the spring action when releasing clear away the button, it finishes resetting to drive and push away material post upward movement, so that next operation, save greatly and punch and connect fungus time, improve the experimental efficiency. The replaceable punching head is made of titanium alloy or stainless steel, so that sterilization can be performed, pollution is reduced, and smooth experiment is guaranteed; meanwhile, the cost of a laboratory is greatly reduced, and the high efficiency of one device with multiple purposes is realized.
Description
Technical Field
The invention belongs to the technical field of microorganism experimental equipment, relates to a culture medium punching sampler, and particularly relates to a punching sampler for a microorganism solid culture medium such as tissues, skin, colloids and leaves.
Background
The hole puncher is a tool widely applied to microbial culture experiments in medicine, agriculture, biology and the like, and in related experiments related to inoculation on a culture dish at present, the common method is to use simple cutting of a scalpel or forceps to transfer bacteria to a new culture dish, the process is easy to cause mixed bacteria pollution in operation and influence the inoculation effect, and due to poor punching, the situations of different punching depths, damaged rubber hole edges, uneven bottom and even being punched can occur, so that the uneven diffusion of a substance to be detected in a rubber block is caused, and the irregular shape of a later-stage antibacterial ring is directly caused; meanwhile, although the currently adopted filter paper sheet method is more in application, certain experimental errors are generated due to different diffusion speeds of the medicaments on the paper sheet, and the experimental progress is influenced.
The sampler is an experimental tool widely used in molecular biology, anatomy and forensic science, and related samplers in the market are various at present, and although the imported Miltex puncher has the advantages of accurate and safe sampling, independent packaging safety and the like, the sampler can not replace a punching sampling head, and different aperture samplers are needed for sampling in different apertures. Although the sampler that punches and release on laboratory and the market mostly can all solve at present and punch and the problem of pushing out the sample, but a lot of samplers are all not suitable for the sterilization, make the culture medium punch and reach aseptic technique, can influence the form of experiment antibacterial zone like this, bring experimental error, influence follow-up experiment.
Disclosure of Invention
The invention aims to solve the technical problem of the prior art, and provides a culture medium punching sampler which is suitable for a puncher for carrying out inoculation work on a culture dish and a sampler for anatomy and legal medical science, can be repeatedly used, reduces pollution, can save punching and inoculation time, improves experiment efficiency, reduces experiment cost and realizes high efficiency of multiple purposes.
The technical scheme of the invention is as follows: a culture medium sampler that punches which characterized in that: the punching sampler is formed by connecting an outer pipe, an inner pipe, a reset spring, a clearing button and a punching sampling head; the utility model discloses a punching sampling device, including outer tube, inner tube, limiting baffle, lower extreme stationary blade, reset spring, lower extreme stationary blade, inner tube, lower extreme stationary blade, lower limiting baffle, lower extreme stationary blade, punching sampling head top outer wall is equipped with the knot hole, punching sampling head bottom is connected and is equipped with the cut-off silk, it colludes to connect to be equipped with the sample on the inner wall of cut-off silk top, the sampling head that punches is detained through the gyration and detains the hole and is connected with the outer tube.
The top of the outer pipe is connected with a handle.
The section cutting wire and the sampling return hook are arranged in a cross-shaped opposite angle mode.
The bottom aperture of the punching sampling head is 1.0 mm-3.0 mm.
The bottom of the material pushing and removing column extends out of the lower fixing piece, and the material pushing and removing column is U-shaped.
The outer pipe outside and clear away the button outside and all be equipped with anti-skidding texture.
And the upper limiting partition plate and the lower limiting partition plate are both provided with exhaust holes.
The handle is arranged on the outer side of the upper end fixing piece, and anti-skidding textures are arranged on the handle.
The invention has the beneficial effects that: the culture medium punching sampler provided by the invention is structurally formed by connecting an outer pipe, an inner pipe, a reset spring, a clearing button and a punching sampling head, the replaceable punching head connected with the outer pipe is used for sampling in a solid culture medium and a sample, the residual agar culture medium and the sample in the punching head are pushed out through the inner pipe after the sample is taken, the punching heads with different apertures are replaced for punching according to different punching sampling requirements, and the reset spring in the inner pipe moves upwards under the action of elasticity to clear and push a material pushing column to complete resetting under the action of elasticity when the clearing button is released, so that the next operation is facilitated, the punching and bacterium receiving time is greatly saved, and the experimental efficiency is improved. The replaceable punching head is made of titanium alloy or stainless steel, so that sterilization can be performed, pollution is reduced, and smooth experiment is guaranteed; meanwhile, the cost of a laboratory is greatly reduced, and the high efficiency of one device with multiple purposes is realized.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
FIG. 2 is a schematic structural diagram of the punch sampling head of the present invention.
In the figure: clear away button 1, inner tube 2, reset spring 3, go up spacing baffle 4, lower spacing baffle 5, clear away and push away material post 6, outer tube 7, detain 8, handle 9, punch the sampling head 10, connect the cutting wire 11, sample and return and collude 12, upper end stationary blade 13, lower extreme stationary blade 14, detain hole 15.
Detailed Description
The invention will be further described with reference to the accompanying drawings in which:
as shown in the figure 1-2, the culture medium perforating sampler is formed by connecting an outer tube 7, an inner tube 2, a return spring 3, a clearing button 1 and a perforating sampling head 10; the inside upper end stationary blade 13 and the lower extreme stationary blade 14 of being equipped with of outer tube 7, inner tube 2 sets up between upper end stationary blade 13 and lower extreme stationary blade 14, upper end stationary blade 13 is worn out to 2 upper ends of inner tube, 2 top connections of inner tube are equipped with clear button 1, 2 lower extremes of inner tube and lower extreme stationary blade 14 fixed connection just communicate with each other, 2 inside spacing baffles 4 and the lower spacing baffle 5 of being equipped with of inner tube, be equipped with reset spring 3 between spacing baffle 4 and the lower spacing baffle 5, the bottom connection of lower spacing baffle 5 is equipped with clears away and pushes away the stock column 6, 14 bottom connections of lower extreme stationary blade are equipped with back-off 8, be equipped with on the sample head 10 top outer wall of punching and detain hole 15, sample head 10 bottom connections of punching is equipped with cuts off silk 11, it is equipped with sample back-off 12 to connect on the inner wall above the cutting off silk, the sample head of punching is connected with outer tube 7 through back-off 8 and detain hole 15.
As shown in the figure 1-2, the top of the outer tube 7 of the culture medium perforating sampler is connected with a handle 9; the section cutting wire 11 and the sampling back hook 12 are arranged in a cross-shaped opposite angle; the bottom aperture of the punching sampling head 10 is 1.0 mm-3.0 mm; the bottom of the removing and pushing column 6 extends out of the lower end fixing piece 14, and the removing and pushing column 6 is U-shaped; anti-skid textures are arranged outside the outer pipe 7 and outside the cleaning button 1; the upper limiting clapboard 4 and the lower limiting clapboard 5 are both provided with exhaust holes; the outer side of the upper end fixing piece 13 is provided with a handle 9, and the handle 9 is provided with anti-skid grains.
As shown in FIGS. 1-2, a media punch sampler operates as follows: the outer tube main part of culture medium sampler that punches is a circular shape stereoplasm outer tube for a cross section, and the stereoplasm outer tube adopts the PE material and is equipped with anti-skidding texture, and there is the stereoplasm inner tube in the inside of stereoplasm outer tube, and the stereoplasm inner tube adopts aluminum alloy or titanium alloy material, and the stereoplasm inner tube is inside to be equipped with can follow the post of cleaing away of stereoplasm inner tube axle direction motion, and the post of should cleaing away is U type and the size of cross section size and inner tube cross section equals. Clear away and push away the stub bar and clear away the button and link to each other, clear away and have anti-skidding texture on the button, prevent to skid when pressing. There is the anti-drop handle on the stereoplasm outer tube, and there is inner tube upper end stationary blade on the handle looks plane, and the stationary blade is equipped with the gas pocket, avoids long-time the use to have the air influence to press. The inner tube has reset spring, rebounds automatically when the button is loosened, convenient operation improves experimental efficiency. Spacing baffle effectively prevents to press excessively about the spring, and the outer tube bottom is equipped with the outer tube cap, not only can prevent the pollution of sampler of punching, can prevent the equipment loss moreover. The sampling head that punches is removable punching head, punching head adopts stereoplasm stainless steel or titanium alloy, punching head upper end is thicker, can guarantee the fixed of rebate, punching head need not to hand can directly change in punching head box, punching head bottom is equipped with diagonal angle connection dissection silk and sample and returns and colludes, it should not block the downward material that pushes away of clear away the post to connect the dissection silk, the sample returns that colludes and colludes towards inner tube upper end, punching head aperture has 1.0mm, 1.5mm, 2.0mm, 3.0 mm's removable punching head, punching head can directly carry out pasteurization. The use process comprises the following steps: the outer tube is held by hand, the outer tube cap is taken down firstly, the sterilized punching head is taken out, the punching sampler is inserted into the punching head for 90 degrees and then rotates anticlockwise, and the punching head is locked by the lock catch. The head of will punching again inserts solid medium or needs sample perpendicularly 90, and at this moment, the connection of the head of punching cut the silk can cut off culture medium or sample rapidly and block in the head of punching, and solid medium or sample are by a cake type of punching head excision, and the sampler of punching is pulled out perpendicularly after the sample of punching, makes through pressing the clear button and clears away post downstream for clear away the post and will remain residual culture medium or sample extrusion in the head of punching. After the punching head is used up, the punching head is taken down and placed into the punching head box for independent storage. And finally, covering an outer pipe cap. The clearing column can automatically rebound and reset under the action of elastic force. The culture medium punching sampler can perform the next punching sampling operation again. The culture medium punching sampler can ensure that cut solid culture medium and samples do not stay on a culture flat plate and are not embedded in a punching head.
Claims (8)
1. A culture medium sampler that punches which characterized in that: the punching sampler is formed by connecting an outer pipe (7), an inner pipe (2), a reset spring (3), a clearing button (1) and a punching sampling head (10); the inner tube (2) is arranged between the upper end fixing piece (13) and the lower end fixing piece (14), the upper end of the inner tube (2) is penetrated out of the upper end fixing piece (13), a clearing button (1) is connected and arranged at the top of the inner tube (2), the lower end of the inner tube (2) is fixedly connected and communicated with the lower end fixing piece (14), an upper limiting baffle plate (4) and a lower limiting baffle plate (5) are arranged inside the inner tube (2), a reset spring (3) is arranged between the upper limiting baffle plate (4) and the lower limiting baffle plate (5), a material pushing column (6) is arranged and arranged at the bottom of the lower limiting baffle plate (5), a back buckle (8) is arranged at the bottom of the lower end fixing piece (14), and a buckle hole (15) is formed in the outer wall of the top of the punching sampling head (10), the punching sampling head is characterized in that a cutting wire (11) is connected to the bottom of the punching sampling head (10), a sampling back hook (12) is connected to the inner wall above the cutting wire, and the punching sampling head is connected with the outer tube (7) through a back buckle (8) and a buckle hole (15).
2. A media punch-sampler according to claim 1 wherein: the top of the outer tube (7) is connected with a handle (9).
3. A media punch-sampler according to claim 1 wherein: the section cutting wire (11) and the sampling return hook (12) are arranged in a cross-shaped opposite angle mode.
4. A media punch-sampler according to claim 1 wherein: the bottom aperture of the punching sampling head (10) is 1.0 mm-3.0 mm.
5. A media punch-sampler according to claim 1 wherein: the bottom of the removing and pushing column (6) extends out of the lower end fixing piece (14), and the removing and pushing column (6) is U-shaped.
6. A media punch-sampler according to claim 1 wherein: the outer part of the outer pipe (7) and the outer part of the cleaning button (1) are both provided with anti-skid textures.
7. A media punch-sampler according to claim 1 wherein: and the upper limiting partition plate (4) and the lower limiting partition plate (5) are both provided with exhaust holes.
8. The media punch-sampler of claim 1, wherein: the outer side of the upper end fixing piece (13) is provided with a handle (9), and anti-skidding textures are arranged on the handle (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110466560.6A CN113174319A (en) | 2021-04-28 | 2021-04-28 | Culture medium sampler that punches |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110466560.6A CN113174319A (en) | 2021-04-28 | 2021-04-28 | Culture medium sampler that punches |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113174319A true CN113174319A (en) | 2021-07-27 |
Family
ID=76926753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110466560.6A Pending CN113174319A (en) | 2021-04-28 | 2021-04-28 | Culture medium sampler that punches |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113174319A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114149900A (en) * | 2021-11-30 | 2022-03-08 | 中国科学院天津工业生物技术研究所 | Cutting head and hypha cutting method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6393926B1 (en) * | 2000-05-19 | 2002-05-28 | Accutrol Co., Inc. | Front-loading precision material sampler with interchangeable retracting chamber |
| CN107139260A (en) * | 2017-05-26 | 2017-09-08 | 李天� | A kind of culture medium punching and markers work platform |
| CN206748588U (en) * | 2017-06-08 | 2017-12-15 | 钟金凤 | A kind of culture medium card punch |
| CN208776702U (en) * | 2018-07-11 | 2019-04-23 | 中国农业大学 | A kind of solid medium punch |
-
2021
- 2021-04-28 CN CN202110466560.6A patent/CN113174319A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6393926B1 (en) * | 2000-05-19 | 2002-05-28 | Accutrol Co., Inc. | Front-loading precision material sampler with interchangeable retracting chamber |
| CN107139260A (en) * | 2017-05-26 | 2017-09-08 | 李天� | A kind of culture medium punching and markers work platform |
| CN206748588U (en) * | 2017-06-08 | 2017-12-15 | 钟金凤 | A kind of culture medium card punch |
| CN208776702U (en) * | 2018-07-11 | 2019-04-23 | 中国农业大学 | A kind of solid medium punch |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114149900A (en) * | 2021-11-30 | 2022-03-08 | 中国科学院天津工业生物技术研究所 | Cutting head and hypha cutting method |
| CN114149900B (en) * | 2021-11-30 | 2024-03-29 | 中国科学院天津工业生物技术研究所 | Cutting head and mycelium cutting method |
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