CN114149900A - Cutting head and hypha cutting method - Google Patents
Cutting head and hypha cutting method Download PDFInfo
- Publication number
- CN114149900A CN114149900A CN202111442956.3A CN202111442956A CN114149900A CN 114149900 A CN114149900 A CN 114149900A CN 202111442956 A CN202111442956 A CN 202111442956A CN 114149900 A CN114149900 A CN 114149900A
- Authority
- CN
- China
- Prior art keywords
- cutting
- cutting head
- head body
- bacterial colony
- hyphae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005520 cutting process Methods 0.000 title claims abstract description 187
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000009826 distribution Methods 0.000 claims abstract description 9
- 230000009471 action Effects 0.000 claims abstract description 5
- 230000001580 bacterial effect Effects 0.000 claims description 27
- 229920001817 Agar Polymers 0.000 claims description 13
- 239000008272 agar Substances 0.000 claims description 13
- 244000005700 microbiome Species 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 4
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 238000007664 blowing Methods 0.000 claims description 2
- 239000002028 Biomass Substances 0.000 abstract description 15
- 238000000855 fermentation Methods 0.000 abstract description 11
- 230000004151 fermentation Effects 0.000 abstract description 11
- 238000013537 high throughput screening Methods 0.000 abstract description 7
- 239000011148 porous material Substances 0.000 description 9
- 241000233866 Fungi Species 0.000 description 7
- 238000012360 testing method Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 241000186361 Actinobacteria <class> Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000003708 ampul Substances 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 229960004756 ethanol Drugs 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 229940093915 gynecological organic acid Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 235000012431 wafers Nutrition 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
本发明涉及一种切取头及菌丝的切取方法，所述切取头包括切取头本体，所述切取头本体为空心筒体，所述切取头本体包括切削部和连接部，所述切削部连接在所述连接部的底端，用于执行切取动作，所述连接部用于与机械臂连接。所述切取头及菌丝的切取方法能够实现菌丝的均匀切取和分配，使生物量均匀地分配到孔板中，保证各孔中生物量的一致，提高发酵结果的可比性，通过微生物的高通量筛选方法找出最优的菌株。
The invention relates to a cutting head and a method for cutting mycelium. The cutting head comprises a cutting head body, the cutting head body is a hollow cylinder, and the cutting head body comprises a cutting part and a connecting part, and the cutting part is connected to The bottom end of the connecting part is used for performing the cutting action, and the connecting part is used for connecting with the robot arm. The cutting head and the cutting method of the mycelium can realize the uniform cutting and distribution of the mycelium, so that the biomass is evenly distributed into the orifice plate, ensuring the consistency of the biomass in each hole, and improving the comparability of the fermentation results. High-throughput screening methods to find the optimal strains.
Description
Technical Field
The invention relates to the field of experimental equipment, in particular to a cutting head and a cutting method of hyphae.
Background
The microorganism can be used for producing various products such as enzyme preparations, organic acids, amino acids, antibiotics, vitamins, hormones, ethanol, biological materials and the like, and the excellent strain is the foundation for realizing the high yield of the microbial fermentation. Both mutagenesis and high-throughput editing can generate a large number of candidate strains, and high-throughput screening is an important means for efficiently and rapidly screening more excellent strains from a plurality of candidate strains.
In the High Throughput Screening (HTS) technology for microorganisms, a pore plate or an agar plate is used as a culture carrier of a strain, an advanced modern automatic operating system is used for executing an experimental process, a sensitive and rapid detection instrument is used for collecting experimental data, a large number of microbial strains are cultured and tested in a short time, and a more excellent strain is found.
Biomass is an important factor of fermentation, and the fermentation performance of the plate method test microorganisms is closely related to the biomass in the wells, and if the biomass is not uniformly distributed to the same batch of plate, the comparability of the fermentation result is poor, and thus, the uniformity of the biomass in each well should be ensured.
However, uniform picking and distribution of hyphae/mycelium is a bottleneck for high throughput screening of actinomycetes, filamentous fungi and macrofungi (the patent collectively refers to actinomycetes, filamentous fungi and macrofungi as "filamentous fungi"). Due to the morphological characteristics of the filamentous fungi, instruments and methods suitable for single cells, such as the existing liquid transfer workstation, the flow cytometry technology, the microfluidic technology and the like, can not realize uniform picking and distribution of hyphae. Some methods adopt a method of picking up hyphae of filamentous fungi by winding a hypha picking thread and then distributing the hyphae into a deep hole plate, and the winding mode cannot realize uniform picking and distribution of the hyphae.
Disclosure of Invention
In view of the above problems, a first object of the present invention is to provide a cutting head capable of uniformly cutting hyphae or mycelia. Another object of the present invention is to provide a hypha cutting method, which can uniformly cut hypha, put into a pore plate for a contrast experiment, and further screen out and find out the optimal bacterial strain.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a cutting head, which includes a cutting part and a connecting part, wherein the cutting part is connected to a bottom end of the connecting part, a cutting edge is formed at the bottom end of the cutting part for performing a cutting operation, and the connecting part is used for connecting with a mechanical arm.
Further, the cutting head body is made of resin or plastic.
Further, the cutting head body is a hollow cylinder.
Furthermore, the inside of the hollow cylinder body is also provided with a filter layer, and the filter layer can only be used for air to pass through and is used for filtering microorganisms in the air, so that the sterile state is realized.
Further, the diameter of the cutting portion is different from the diameter of the connecting portion.
The cutting head comprises a cutting head body and is characterized by further comprising an outer layer cutting sleeve sleeved outside the cutting head body, the inner wall of the outer layer cutting sleeve is attached to the outer wall of the cutting head body, the outer wall of the outer layer cutting sleeve and the outer wall of the cutting head body can rotate mutually, and a cutting edge is formed at the bottom of the outer layer cutting head body.
In a second aspect, the present invention also provides a method for cutting a hyphae, based on the cutting head, comprising the steps of:
mounting the cutting head on an automatic cutting machine, and aligning a cutting part of the cutting head to one area of the edge of the bacterial colony;
cutting a circular plate from the bacterial colony together with the culture medium to form a bacterial colony plate, and simultaneously extracting gas in the cutting head body to form negative pressure in the cutting head body so as to prevent the bacterial colony plate from falling off;
moving the cut colony piece with the cutting head to a culture hole or an agar plate to be inoculated, introducing a small amount of air into the cutting head pipe, and blowing the colony piece into the culture hole or onto the agar plate;
repeating the operation to cut and distribute hyphae.
Furthermore, the cutting head also comprises an outer layer cutting sleeve sleeved outside the cutting head body, the inner wall of the outer layer cutting sleeve is attached to the outer wall of the cutting head body, the outer layer cutting sleeve and the outer wall of the cutting head body can rotate mutually, and a cutting edge is formed at the bottom of the outer layer cutting head body;
the step of cutting a disc from the bacterial colony together with the culture medium to form a bacterial colony specifically comprises the following steps:
through the arm court the bacterial colony pushes down the cutting head to through cutting head body or outer cutting sleeve rotate in order to ensure to cut off the hypha.
Due to the adoption of the technical scheme, the invention has the following advantages:
the cutting head provided by the invention has the advantages that as the inner diameters of the cutting parts are the same, the diameters of cut wafers are the same, and uniform cutting and distribution of hyphae are realized;
the cutting head provided by the invention comprises a cutting head body and an outer layer cutting sleeve sleeved on the outer side of the cutting head body, and relative rotation is formed between the cutting head body and the outer layer cutting sleeve, so that hyphae can be cut off by the rotation of the cutting head body or the outer layer cutting head body in the cutting process.
The invention can be used for transferring filamentous fungi, and hyphae are cut and inoculated into agar plates/pore plates/deep pore plates/test tubes/triangular flasks or other suitable culture containers. Or transferring the cut hyphae to a test tube/a freezing tube/an ampoule tube to carry out the automatic operation of strain preservation.
Drawings
FIG. 1 is a schematic cross-sectional view of a first embodiment of a cutting head provided in accordance with the present invention;
FIG. 2 is a schematic cross-sectional view of a cutting head provided in accordance with the present invention;
FIG. 3 is a schematic cross-sectional view of a second embodiment of a cutting head provided in accordance with the present invention;
description of reference numerals:
1-cutting head body, 2-filtering layer, 11-cutting part, 12-connecting part and 3-outer layer cutting sleeve.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the following will clearly and completely describe the technical solutions of the present invention with reference to the accompanying drawings. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inside", "outside", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the system or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used to define elements only for convenience in distinguishing between the elements, and unless otherwise stated have no special meaning and are not to be construed as indicating or implying any relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The embodiment of the invention provides a cutting head and a hypha cutting method, which can realize uniform cutting and distribution of hypha, uniformly distribute biomass into a pore plate, ensure the consistency of the biomass in each pore, improve the comparability of fermentation results and find out an optimal strain by a high-throughput screening method of microorganisms.
Example 1
The cutting head provided by the embodiment 1 of the invention comprises a cutting head body 1, wherein the cutting head body 1 is a hollow cylinder, the cutting head body 1 comprises a cutting part 11 and a connecting part 12, the cutting part 11 is connected to the bottom end of the connecting part and is used for executing cutting action, and the connecting part 12 is used for being connected with a mechanical arm (not shown in the figure).
The cutting head body 1 is preferably made of resin or plastic, and the cutting head body 1 may be made of stainless steel or other materials.
The cutting head body 1 is preferably a hollow cylinder. It is understood that the cutting head body 1 is not limited to a hollow cylinder, but may be a square or polygonal cylinder.
The cutting head body 1 has a cutting portion 11 at the bottom thereof, and the cutting portion 11 has a sharp tip, and is capable of pressing a culture medium and colonies under a certain pressure and cutting the colonies together with the culture medium.
In order to avoid pollution caused by microorganisms and the like in the process of cutting and transferring hyphae, a filter layer 2 is fixedly arranged in the hollow cylinder, and the filter layer 2 can be used for filtering the microorganisms in the gas through the gas.
The top of the connecting portion 12 is connected to a robot arm (not shown) which performs the cutting and transferring actions on the cutting head. The top of the connecting part 12 and the mechanical arm may be connected by bolts, plugs or other detachable connections, preferably plugs.
As one embodiment, the diameter of the cutting portion 11 is different from the diameter of the connecting portion 12, and as shown in fig. 2, the outer diameter and the inner diameter of the cutting portion 11 are both larger than the outer diameter and the inner diameter of the connecting portion 12.
Based on the above cutting head, another embodiment of the present invention provides a method for cutting hyphae, comprising the steps of:
(1) inoculating spores or hyphae of the candidate strain on an agar plate;
(2) growing the candidate strain into a colony on an agar plate;
(3) mounting a cutting head on a mechanical arm of an automatic cutting machine;
(4) aligning a cutting head to an area at the edge of a bacterial colony, cutting a round plate (called as a bacterial colony plate) from the bacterial colony together with a culture medium, and simultaneously extracting gas in the cutting head body 1 to form negative pressure in the cutting head body 1 so as to prevent the bacterial colony plate from falling off;
(5) the cutting head carries the cut bacterial colony sheet to move to a culture hole or an agar plate to be inoculated, a small amount of air is introduced into a cylinder body of the cutting head, and the bacterial colony sheet is blown into the culture hole or blown down on the agar plate;
(6) repeating the operation, finishing the cutting and distribution of hyphae, replacing a sterile cutting head every time, and selecting a hyphae area with the same or similar density at the edge of a bacterial colony every time through digital photography and machine vision so as to ensure that the biomass of the cut bacterial colony is the same or similar.
When the density of the mycelia at the edges of the colonies is different, the percentage of the mycelia on the colony sheets to the area on the colony sheets is controlled to realize the same or similar biomass on each cut colony sheet. If the density of mycelia at the edge of the colony is too low, two or more pieces may be cut so that the biomass obtained from the strain is the same as or similar to that of other bacteria.
It should be noted that the automatic cutter is mainly composed of a mechanical arm, etc., and can automatically change the cutting head according to the program to cut the fungus drop at the edge of the colony selected by digital photography and machine vision. The mechanical arm of the automatic cutting machine controls the falling force (the pressure contacting with the flat plate) of the cutting head through the spring and/or the lever, so that the cutting head is cut to the surface of a vessel below the agar flat plate to complete the cutting of a bacterial colony sheet, and the action, the transfer and the force of the cutting head are controlled in detail, which belongs to the prior art in the field, and therefore the details are omitted.
The above-mentioned excised colony pieces are used for inoculation into agar plates/well plates/deep well plates/test tubes/flasks or other suitable culture vessels. Or transferring the cut colony slices into a test tube/a freezing tube/an ampoule tube to carry out the automatic operation of strain preservation.
The cutting head and the cutting method of the hyphae provided by the invention can realize the uniform cutting and distribution of the hyphae, uniformly distribute the biomass into the pore plates, ensure the consistency of the biomass in each pore, improve the comparability of a fermentation result, and find out the optimal strain by a high-throughput screening method of microorganisms.
The invention has the advantages that the operation is greatly simplified, the uniform sampling and the uniform distribution of the hyphae are realized, and simultaneously: (1) because the strain is not required to grow spores, the method is suitable for strains which do not produce spores; (2) the strain does not need to grow spores, so that the culture time of the strain on an agar plate is short; (3) hyphae at the edge of a colony are cut, and the part of hyphae is new hyphae, so that the mycelium fermentation of different strains is easy to realize synchronization; (4) because the biomass on each sliced colony slice is the same or similar with the assistance of digital photography and machine vision, the biomass in each fermentation pore plate can be consistent, and parallel experiments or comparison of different experiments can be implemented; (5) cross contamination is not easy to occur; (6) in the subsequent fermentation stage, the spore germination stage is not needed, so that the experimental period of fermentation is shortened.
Example 2
The difference between embodiment 2 and embodiment 1 of the present invention is that the cutting head in this embodiment is a double-layer cutting head, an outer layer cutting sleeve 3 is further sleeved on an outer layer of the cutting head body in embodiment 1, an inner wall of the outer layer cutting sleeve 3 is attached to an outer wall of the cutting head body 1, the inner wall and the outer wall can rotate with each other, and a bottom of the outer layer cutting sleeve 3 is sharp.
Therefore, in the hypha cutting process, the cutting of a disc from the bacterial colony together with the culture medium to form a bacterial colony sheet specifically comprises the following steps:
through the arm court the bacterial colony pushes down the cutting head to it is rotatory cutting head body 1 or outer cutting sleeve 3 are in order to ensure to cut off the hypha.
Therefore, in the invention, the cutting head is arranged to be of a double-layer structure sleeved inside and outside, so that hyphae can be cut off by rotating the cutting head body 1 at the inner layer or rotating the cutting sleeve 3 at the outer layer.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
1. The utility model provides a cutting head, its characterized in that, includes the cutting head body, the cutting head body is hollow barrel, the cutting head body includes cutting part and connecting portion, the cutting part is connected the bottom of connecting portion, the bottom of cutting part is formed with the cutting edge for carry out the cutting action, connecting portion are used for being connected with the arm.
2. The cutting head according to claim 1, wherein the cutting head body is made of resin or plastic.
3. The cutting head according to claim 1, wherein the cutting head body is a hollow cylinder.
4. The cutting head according to claim 1, wherein a filter layer is further provided inside the hollow cylinder, and the filter layer is only capable of allowing gas to pass through and is used for filtering microorganisms in the gas to realize a sterile state.
5. The cutting head according to claim 1, wherein the diameter of the cutting portion is different from the diameter of the connecting portion.
6. The cutting head according to claim 1, further comprising an outer cutting sleeve disposed outside the cutting head body, wherein an inner wall of the outer cutting sleeve is engaged with an outer wall of the cutting head body and is rotatable with respect to the outer cutting sleeve, and a cutting edge is formed at a bottom of the outer cutting head body.
7. A method for cutting out hyphae, comprising the step of:
mounting the cutting head on an automatic cutting machine, and aligning a cutting part of the cutting head to one area of the edge of the bacterial colony;
cutting a circular plate from the bacterial colony together with the culture medium to form a bacterial colony plate, and simultaneously extracting gas in the cutting head body to form negative pressure in the cutting head body so as to prevent the bacterial colony plate from falling off;
moving the cut colony piece with the cutting head to a culture hole or an agar plate to be inoculated, introducing a small amount of air into the cutting head pipe, and blowing the colony piece into the culture hole or onto the agar plate;
the above operation is repeated to complete the distribution of hyphae.
8. The method for cutting off hyphae according to claim 7, wherein the cutting head further comprises an outer cutting sleeve sleeved outside the cutting head body, the inner wall of the outer cutting sleeve is attached to the outer wall of the cutting head body and can rotate with each other, and a cutting edge is formed at the bottom of the outer cutting head body;
the step of cutting a disc from the bacterial colony together with the culture medium to form a bacterial colony specifically comprises the following steps:
through the arm court the bacterial colony pushes down the cutting head to through cutting head body or outer cutting sleeve rotate in order to ensure to cut off the hypha.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111442956.3A CN114149900B (en) | 2021-11-30 | 2021-11-30 | Cutting head and mycelium cutting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111442956.3A CN114149900B (en) | 2021-11-30 | 2021-11-30 | Cutting head and mycelium cutting method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114149900A true CN114149900A (en) | 2022-03-08 |
| CN114149900B CN114149900B (en) | 2024-03-29 |
Family
ID=80454865
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111442956.3A Active CN114149900B (en) | 2021-11-30 | 2021-11-30 | Cutting head and mycelium cutting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114149900B (en) |
Citations (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07107965A (en) * | 1993-10-13 | 1995-04-25 | Nittetsu Mining Co Ltd | Method and device for perforating agar medium |
| CN202053259U (en) * | 2011-03-28 | 2011-11-30 | 林建邦 | Cutting tool |
| CN102321522A (en) * | 2011-09-23 | 2012-01-18 | 安徽农业大学 | Cell scraper |
| CN203490097U (en) * | 2013-09-25 | 2014-03-19 | 新乡学院 | Hole puncher for experiment on microbiology |
| CN203715617U (en) * | 2014-01-16 | 2014-07-16 | 菏泽学院 | Bacterial colony moving and taking device |
| CN204014925U (en) * | 2014-08-22 | 2014-12-17 | 吴元彪 | A kind of cake cuts cutter |
| CN104232477A (en) * | 2014-09-12 | 2014-12-24 | 天津蓠原农业生态科技有限公司 | Aseptic sampling device for biological sample |
| CN204298403U (en) * | 2014-10-21 | 2015-04-29 | 中国热带农业科学院南亚热带作物研究所 | A kind of bacterium dish is beaten and is got device |
| CN204474666U (en) * | 2015-02-06 | 2015-07-15 | 吕益飞 | A kind of novel microorganism Gelose punch |
| CN204566263U (en) * | 2015-04-29 | 2015-08-19 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of movable sleeve tubular type agar plate card punch |
| CN204622198U (en) * | 2015-03-23 | 2015-09-09 | 上海源耀生物股份有限公司 | A kind of inoculation agar plate can sterilizing card punch |
| CN204752720U (en) * | 2015-05-07 | 2015-11-11 | 金华职业技术学院 | Fungus cake hole puncher |
| CN205368365U (en) * | 2016-01-15 | 2016-07-06 | 安庆师范学院 | Collector is cultivateed to filamentous fungi hypha |
| CN208733111U (en) * | 2018-06-25 | 2019-04-12 | 广西农业职业技术学院 | A kind of bacteria cake punch |
| CN209584222U (en) * | 2019-01-31 | 2019-11-05 | 福建农林大学 | Portable microbial hole punch |
| CN209611242U (en) * | 2019-01-29 | 2019-11-12 | 美闺(长沙)医疗美容有限公司 | A kind of cosmetic surgery nasal plastic cartilage extractor |
| CN209906783U (en) * | 2019-05-09 | 2020-01-07 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Novel microorganism agar hole puncher |
| CN210287341U (en) * | 2019-07-24 | 2020-04-10 | 延边兴林生物科技有限公司 | Push type inoculator that punches |
| CN210796461U (en) * | 2019-09-21 | 2020-06-19 | 淄博市农业科学研究院 | Microbial inoculation tool |
| CN211367558U (en) * | 2019-11-15 | 2020-08-28 | 陕西麦可罗生物科技有限公司 | Microorganism single colony separation instrument that punches |
| CN212601678U (en) * | 2020-07-28 | 2021-02-26 | 海南省农业科学院植物保护研究所 | Detachable fungus hypha scraper |
| CN212833770U (en) * | 2019-12-30 | 2021-03-30 | 东北林业大学 | An integrated device for inoculating bacteria and punching holes |
| CN113106006A (en) * | 2021-04-09 | 2021-07-13 | 江苏滋百农生态农业股份有限公司 | Functional bacterium quantitative determination arm |
| CN113174319A (en) * | 2021-04-28 | 2021-07-27 | 扬州大学 | Culture medium sampler that punches |
| CN213895838U (en) * | 2020-11-19 | 2021-08-06 | 陕西麦可罗生物科技有限公司 | Instrument is got in single fungus piece of microorganism moving |
| CN113265320A (en) * | 2021-05-17 | 2021-08-17 | 杭州职业技术学院 | Uniform silk taking device for culture medium and use method |
-
2021
- 2021-11-30 CN CN202111442956.3A patent/CN114149900B/en active Active
Patent Citations (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07107965A (en) * | 1993-10-13 | 1995-04-25 | Nittetsu Mining Co Ltd | Method and device for perforating agar medium |
| CN202053259U (en) * | 2011-03-28 | 2011-11-30 | 林建邦 | Cutting tool |
| CN102321522A (en) * | 2011-09-23 | 2012-01-18 | 安徽农业大学 | Cell scraper |
| CN203490097U (en) * | 2013-09-25 | 2014-03-19 | 新乡学院 | Hole puncher for experiment on microbiology |
| CN203715617U (en) * | 2014-01-16 | 2014-07-16 | 菏泽学院 | Bacterial colony moving and taking device |
| CN204014925U (en) * | 2014-08-22 | 2014-12-17 | 吴元彪 | A kind of cake cuts cutter |
| CN104232477A (en) * | 2014-09-12 | 2014-12-24 | 天津蓠原农业生态科技有限公司 | Aseptic sampling device for biological sample |
| CN204298403U (en) * | 2014-10-21 | 2015-04-29 | 中国热带农业科学院南亚热带作物研究所 | A kind of bacterium dish is beaten and is got device |
| CN204474666U (en) * | 2015-02-06 | 2015-07-15 | 吕益飞 | A kind of novel microorganism Gelose punch |
| CN204622198U (en) * | 2015-03-23 | 2015-09-09 | 上海源耀生物股份有限公司 | A kind of inoculation agar plate can sterilizing card punch |
| CN204566263U (en) * | 2015-04-29 | 2015-08-19 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of movable sleeve tubular type agar plate card punch |
| CN204752720U (en) * | 2015-05-07 | 2015-11-11 | 金华职业技术学院 | Fungus cake hole puncher |
| CN205368365U (en) * | 2016-01-15 | 2016-07-06 | 安庆师范学院 | Collector is cultivateed to filamentous fungi hypha |
| CN208733111U (en) * | 2018-06-25 | 2019-04-12 | 广西农业职业技术学院 | A kind of bacteria cake punch |
| CN209611242U (en) * | 2019-01-29 | 2019-11-12 | 美闺(长沙)医疗美容有限公司 | A kind of cosmetic surgery nasal plastic cartilage extractor |
| CN209584222U (en) * | 2019-01-31 | 2019-11-05 | 福建农林大学 | Portable microbial hole punch |
| CN209906783U (en) * | 2019-05-09 | 2020-01-07 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Novel microorganism agar hole puncher |
| CN210287341U (en) * | 2019-07-24 | 2020-04-10 | 延边兴林生物科技有限公司 | Push type inoculator that punches |
| CN210796461U (en) * | 2019-09-21 | 2020-06-19 | 淄博市农业科学研究院 | Microbial inoculation tool |
| CN211367558U (en) * | 2019-11-15 | 2020-08-28 | 陕西麦可罗生物科技有限公司 | Microorganism single colony separation instrument that punches |
| CN212833770U (en) * | 2019-12-30 | 2021-03-30 | 东北林业大学 | An integrated device for inoculating bacteria and punching holes |
| CN212601678U (en) * | 2020-07-28 | 2021-02-26 | 海南省农业科学院植物保护研究所 | Detachable fungus hypha scraper |
| CN213895838U (en) * | 2020-11-19 | 2021-08-06 | 陕西麦可罗生物科技有限公司 | Instrument is got in single fungus piece of microorganism moving |
| CN113106006A (en) * | 2021-04-09 | 2021-07-13 | 江苏滋百农生态农业股份有限公司 | Functional bacterium quantitative determination arm |
| CN113174319A (en) * | 2021-04-28 | 2021-07-27 | 扬州大学 | Culture medium sampler that punches |
| CN113265320A (en) * | 2021-05-17 | 2021-08-17 | 杭州职业技术学院 | Uniform silk taking device for culture medium and use method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114149900B (en) | 2024-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101671625A (en) | Method and device for preparing trichoderma conidium by liquid state submerged fermentation | |
| CN105483204A (en) | Detection vessel for rapidly detecting moulds and yeasts and preparation method | |
| CN114507660A (en) | High-throughput breeding method for ultrahigh-concentration beer brewing strains | |
| Barry et al. | Morphological quantification of filamentous fungal development using membrane immobilization and automatic image analysis | |
| CN112980691A (en) | Method for rapidly and accurately screening plant pathogenic fungi antagonistic bacterial strain | |
| CN114149900A (en) | Cutting head and hypha cutting method | |
| CN110684763A (en) | Mutagenesis method of volvariella volvacea spores | |
| CN104762207A (en) | Special culture membrane for single cell cloning separation and single cell cloning separation method | |
| CN109839380A (en) | A kind of pelletizer and method for filamentous fungi morphological feature microexamination | |
| CN118580942A (en) | A foodborne microorganism inspection device and inspection method | |
| CN104894054A (en) | Monkey embryo renal epithelial cell Marc-145 suspension adapted strain and application thereof in culture of PRRSV (porcine reproductive and respiratory syndrome virus) and production of PRRSV vaccine | |
| WO1984001959A1 (en) | Rapid production of biological products by fermentation in a densely-packed microbial membrane reactor | |
| CN206666533U (en) | The culture dish collected for mycelia | |
| CN215103263U (en) | Colony statistics sticky plastic film for plasmid loss rate experiment | |
| CN212051466U (en) | High-flux cell culture device easy for cell to be detached from wall | |
| CN204608032U (en) | A kind of single cell clone separate, dedicated culture membrane | |
| CN112391273A (en) | Single cell separator, application of single cell separator in single cell separation process and preparation method of monoclonal cells | |
| CN114807008A (en) | Preparation method and application of tomato leaf protoplast single cell suspension | |
| CN114544468A (en) | Capacitance method cell counting method for fixed bed bioreactor | |
| CN110616152A (en) | Preparation method of improved fungus protoplast | |
| CN117551586B (en) | Culture solution for high-flux separation method of plant co-dwelling microorganism and separation method | |
| CN112760259A (en) | Coenzyme Q10 strain expanding culture method | |
| CN219824186U (en) | Fungus head is taken and novel magnetism that contains fungus head is inhaled and is vaccinated ring subassembly | |
| CN220597521U (en) | Porous cell culture plate | |
| CN216808765U (en) | Batch bacteria shaking plate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |