CN201395595Y - A device for smashing gels and collecting DNA solutions - Google Patents

Abstract

A set of utensils for collecting DNA solution from electrophoresis gel, which mainly includes a breaker for crushing electrophoresis gel and a collection tube for collecting DNA-containing liquid. The tail of the breaker is hollow and can be set on the pipette. The head is a cross slice with sharp edges, and the outer contour matches the inside of the collection tube. The diameter of the collection tube body is slightly smaller than that of the centrifuge tube. The nozzle has an outwardly thickened upper edge, and the bottom is arc-shaped, which can be placed in the centrifuge tube. The upper edge is stuck on the centrifuge tube mouth. The distance between the bottom of the collection tube and the bottom of the centrifuge tube is 0.5cm , There are five holes with a diameter of 0.8mm at the bottom of the collection tube. This set of devices can easily and quickly obtain a large amount of DNA-containing solution from the gel.

Description

A device for smashing gels and collecting DNA solutions

technical field

The utility model relates to a set of utensils for collecting DNA solution from electrophoretic gel, in particular to a breaker for crushing electrophoretic gel and a collection tube for collecting liquid containing DNA.

Background technique

At present, some molecular biology experiments need to roughly extract some DNA solutions from DNA electrophoresis gels to prepare for subsequent experiments. Usually, the DNA solution in the electrophoresis gel is collected by smashing it with a hard object, and then sucking the liquid squeezed out of the gel with a pipette. This method has the following disadvantages: 1. There is no suitable tool, the gel is not mashed thoroughly enough, and the extruded DNA liquid is less. 2. The liquid absorbed is not complete enough. 3. It is labor-intensive and time-consuming, and the resulting DNA solution has a small volume and few DNA molecules, which is not conducive to subsequent experiments.

Contents of the invention

In order to overcome the shortcomings of the existing methods for collecting DNA solutions from gels that are laborious and time-consuming and the amount of solution obtained is small, the utility model provides a set of breaker and collection tube device with simple structure and convenient use, which includes a A gel breaker on a liquid dispenser (commonly known as a pipette) and a collection tube that can be sleeved in a centrifuge tube. This set of tools can easily and thoroughly mash the electrophoresis gel and collect all the resulting DNA solution in a centrifuge tube by centrifugation.

The technical solution adopted by the utility model to solve the technical problem is: the tail of the breaker is hollow and can be set on a pipette with a measuring range of 1ml; the head of the breaker is a cross slice with sharp edges, and the outer contour matches the inside of the collection tube , can be freely inserted into the collection tube. The diameter of the collection tube body is slightly smaller than that of the centrifuge tube. The nozzle has an outwardly thickened upper edge, and the bottom is arc-shaped, which can be placed in the centrifuge tube. The upper edge is stuck on the centrifuge tube mouth. The distance between the bottom of the collection tube and the bottom of the centrifuge tube is 0.5cm , There are five holes with a diameter of 0.8mm at the bottom of the collection tube. When operating, put the cut electrophoresis gel block in the collection tube, put the collection tube in the centrifuge tube, insert the breaker on the pipette head, insert the breaker into the collection tube with the pipette in hand, and cut the gel into four pieces, repeatedly inserting and inserting the breaker, the gel is cut into fine pieces, and a large amount of DNA-containing solution is produced during the breaking of the glue pieces, and then the centrifuge tube with the collection tube is placed in the centrifuge for centrifugation. Centrifuge at 1000-5000 rpm for 2-5 minutes, the DNA solution flows to the bottom of the centrifuge tube through the small hole at the bottom of the collection tube, and the broken glue remains in the collection tube because it is too large to pass through the small hole, remove the collection tube , what remains in the centrifuge tube is the solution containing DNA.

The beneficial effect of the utility model is that, compared with the traditional manual collection method, it is simpler and quicker to obtain a large amount of DNA-containing solution from the gel, which is convenient for simple DNA-related biological experiments, such as simple ligation reactions, PCR products TA cloning and a small amount of DNA recovery.

Description of drawings

Below in conjunction with accompanying drawing and embodiment the utility model is further described.

Fig. 1 is a structural schematic diagram of the side of the utility model crusher

Fig. 2 is a structural schematic diagram of the bottom surface of the utility model crusher

Fig. 3 is a schematic structural view of the utility model collection tube side

Fig. 4 is the structural representation of the bottom surface of the collection pipe of the present invention

Fig. 5 is the structural representation of the utility model using process

In the figure 1. Crusher, 2. Cross slice, 3. Upper edge of collection tube, 4. Small hole

Detailed ways

After DNA electrophoresis is completed, cut out the electrophoresis gel block containing the target DNA band and put it in a collection tube. The collection tube is placed in a 2ml centrifuge tube, insert the breaker (1) on the pipette, and hold the pipette to use Insert the criss-cross slice into the collection tube, cut the gel into four pieces, insert and insert the breaker repeatedly, and cut the gel into fine pieces. At this time, the pieces of gel and DNA solution are in the collection tube. Put the centrifuge tube with the collection tube into the centrifuge, centrifuge at 2000 rpm for 3 minutes, the DNA solution flows to the bottom of the centrifuge tube through the small hole (4) at the bottom of the collection tube, and the broken glue pieces are larger Can not pass through the small hole (4) and still stay in the collection tube, remove the collection tube, what stays in the centrifuge tube is exactly the solution containing DNA.

Claims (2)

1. a cover is collected the utensil of dna solution from running gel, it comprises the destroyer of smashing running gel to pieces and collects the collection tube that contains DNA liquid, it is characterized in that: destroyer afterbody hollow, can be enclosed within on the pipettor, head is the right-angled intersection thin slice, sharp edges, outline and collection tube are inner to coincide; Collection tube pipe shaft diameter is slightly less than centrifuge tube, and the mouth of pipe has the upper edge that outwards thickens, and the bottom is curved, can be enclosed within the centrifuge tube, and the upper edge is stuck on the centrifugal mouth of pipe, and at a distance of 0.5cm, the hole of five diameter 0.8mm is arranged at the collection tube bottom bottom centrifuge tube in the collection tube bottom.

2. be used to the device smashing running gel to pieces and collect dna solution according to claim 1, it is characterized in that collection tube can make the model that varies in size, to satisfy different volume needs.

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