CN217318324U - Gel plate puncher - Google Patents

Abstract

The utility model discloses a gel plate puncher, which comprises a punching capsule and a punching pipe, wherein the punching capsule is divided into a capsule ball and a capsule pipe; the capsule ball is enclosed into a capsule structure by the capsule wall, the top end of the capsule ball is provided with a capsule hole, the lower part of the capsule hole is a capsule cavity, the upper half part of the capsule cavity is a pressurizing cavity, the lower half part of the capsule cavity is a buffer cavity, and the lower end of the capsule ball is tightly connected with the capsule pipe; four punching pipe sleeves with different specifications are sequentially connected in the capsule pipe from top to bottom according to the hole diameters from small to large; the punching pipe sleeves can be matched with four punching pipes with different specifications respectively; the gel plate puncher is in a flower bud shape as a whole. When the gel plate is punched, the operation is carried out according to the conditions that the gel plate is embedded in zero pressure difference, pulled out in negative pressure difference and flicked off in positive pressure difference. The holes are punched in this way, the edges of the holes are neat, no gap is left between the gel plate and the glass slide, no leakage and no diffusion of sample liquid are ensured, and the experimental result is good. The gel plate puncher has the advantages of simple structure, reasonable design, small size, exquisite appearance, convenience and quickness in operation and low cost.

Description

Gel plate puncher

Technical Field

The utility model relates to a field needs such as medical science, prevention, pharmacy, animal and plant carry out the experimental apparatus of immunoelectrophoresis experiment to biological sample, belongs to dull and stereotyped hole puncher technical field, and specifically speaking is a gel board hole puncher.

Background

Immunoelectrophoresis is one of the most commonly used experimental methods in the fields of clinical medicine, preventive medicine, pharmacy, inspection, animal and plant science, etc. It is widely used for experimental research and application of protein separation, identification, disease diagnosis and the like.

The types and the styles of the hole puncher are not few, and the existing gel plate hole puncher, such as Chinese patent CN201320149983.6 'a gel hole puncher', needs to remove the gel in the gel recovery device and clean the pipeline after use; for example, CN200320107319.1, a new agar perforator, has a single perforating pipe, and agar blocks will be sucked into the air bag when improper force is applied; for example, in the Chinese patent, CN02254366.X agar puncher adopts a stainless steel knife, the edge of the knife is sharp and easy to hurt hands, the puncher cannot endure high-pressure steam sterilization, and when the puncher is used, a toothpick is required to pick out an agar block in a puncher tube; for example, in the chinese patent, cn201420306071.x "suction type agar plate puncher" uses a syringe to perform suction, has large resistance, is not easy to control, and has an unnatural technique; similar to a suction type agar plate puncher in the market, the puncher is shaped like a liquid transfer gun, one gun is matched with one punching pipe, and the price is high; also as in the chinese patent, CN201520167685.9, "a sterilizable puncher for agar plates for inoculation", uses a shovel-type cutter head to punch holes, and has irregular shape and uneven edge; for example, in chinese patent, cn201320592980.x, "punch for microorganism experiment", is used for sampling microorganism culture, and the culture is sucked into the capsular sac when force is improperly applied; for example, CN201520268149.8 "a movable sleeve type agar plate puncher" overcomes the defect that punching and taking out agar are performed in two steps, and when the gel is thin and soft, the gel layer is thin, and the gel pore diameter is large, the gel is not easy to take out, and is a disposable consumable, which is wasteful of consumables.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a simple structure, reasonable in design to the not enough of prior art、Small and exquisite, convenient and fast operation, low cost and utility model of gel plate puncher.

Realize the utility model discloses the technical scheme of purpose is:

a gel-plate punch comprising a punch capsule and a punch tube, different from the prior art:

the perforated capsule is divided into a capsule ball and a capsule tube;

the capsule ball is surrounded by the capsule wall to form a capsule structure, the top end of the capsule ball is provided with a capsule hole, the lower part of the capsule hole is a capsule cavity, the upper half part of the capsule cavity is a pressurizing cavity, the lower half part of the capsule cavity is a buffer cavity, and the lower end of the capsule ball is tightly connected with the capsule pipe;

four punching pipe sleeves with different specifications are sequentially connected in the capsule pipe from top to bottom according to the hole diameters from small to large;

the punching pipe sleeves can be matched with four punching pipes with different specifications respectively;

the gel plate puncher is in a flower bud shape as a whole.

The inner diameter of the capsule hole is 2mm, the volume of a pressurizing cavity in the capsule cavity is 200 cubic millimeters, and the volume of a buffer cavity is 800 cubic millimeters.

The inner diameters of the four punching pipe sleeves with different specifications are phi =2mm, 3mm, 4mm and 5mm respectively, the heights of the first punching pipe sleeve, the second punching pipe sleeve and the third punching pipe sleeve are 5mm, and the height of the fourth punching pipe sleeve at the bottom is 10 mm.

The four perforating pipes with different specifications are made of stainless steel pipes, the outer diameters of the four perforating pipes are phi =2mm, 3mm, 4mm and 5mm respectively, the lengths of the four perforating pipes are 50mm, 45mm, 40mm and 35mm respectively, the upper ends of the perforating pipes are flat and matched with perforating pipe sleeves respectively, the lower ends of the perforating pipes are chamfered inwards along the outer diameters, the lower ends of the perforating pipes are sharp, and the lower ends of the perforating pipes are cutting knives for perforating gel sheets.

The perforated capsule is a silica gel capsule, is acid and alkali resistant, can be used for disinfectant disinfection and high-pressure steam sterilization, and can be repeatedly used.

The capsule ball in the gel plate puncher of the utility model is enclosed into a capsule structure by the capsule wall, has rich elasticity and crease resistance, is provided with a capsule hole at the top end, is matched with the index finger abdomen for use, and has the function of adjusting and controlling the air pressure in the capsule cavity and the punching pipe; the upper part of the capsule cavity is a pressurizing cavity which transfers the air pressure generated by the capsule wall to the upper surface of the gel at the lower part of the perforating pipe, and the lower part of the capsule cavity is a buffer cavity which effectively prevents the gel block from moving upwards; the capsule tube is also made of silica gel and is elastic, and the upper part of the capsule tube is tightly connected with the lower part of the capsule ball into a whole.

The utility model discloses gel board hole puncher is digged into its principle, colloquially says, and is embedding during exactly "zero pressure difference, extracts during the negative pressure difference, and the bullet falls during positive pressure difference", realizes its function of punching through control capsule hole and capsule wall. This is the feature of the present invention.

The utility model discloses gel board hole puncher, simple structure, small and exquisite, the material is few, and processing is easy, and is with low costs. The total length is only 7 cm, the maximum diameter is only 2 cm, the punched capsule and the punched pipe are closely matched, and the air tightness is good.

The utility model discloses gel plate hole puncher, reasonable in design, convenient operation. Each part of the gel plate puncher is designed according to the function, and the size, the length, the thickness, the volume and the like of the gel plate puncher meet the actual requirements. The gel plate puncher is in a flower bud shape as a whole, the upper part of a punching pipe is pinched by a thumb and a middle finger during punching, and the capsule hole is controlled by a forefinger, so that the gel plate puncher meets the ergonomic design requirement; the proportion of the pressurizing cavity and the buffer cavity is proper, so that the punching requirement is met; the specification of the perforated pipe meets the requirements of conventional experiments; the punching pipe sleeve and the punching pipe are in seamless joint, are stable and cannot be loosened, and meet the quality requirement.

Drawings

FIG. 1 is a schematic diagram of the construction of an embodiment gel sheet punch;

FIG. 2 is a schematic view of the perforated pipe of FIG. 1;

FIG. 3 is a diagram of gel plate effect;

in the figure, 1, a punched capsule 2, a punched tube 2-1, a first punched tube 2-2, a second punched tube 2-3, a third punched tube 2-4, a fourth punched tube 3, a capsule ball 4, a capsule tube 5, a capsule hole 6, a capsule cavity 6-1, a pressurizing cavity 6-2, a buffer cavity 7, a capsule wall 8, a punched tube sleeve 8-1, a first punched tube sleeve 8-2, a second punched tube sleeve 8-3, a third punched tube sleeve 8-4, a fourth punched tube sleeve 9, a gel plate 10, a glass slide 11 and a gel plate hole are arranged.

Detailed Description

The present invention will be further described with reference to the following examples and drawings, but the present invention is not limited thereto.

Examples

Referring to fig. 1-2, a gel plate puncher comprises a punching capsule 1 and a punching tube 2, wherein the punching capsule 1 is divided into a capsule ball 3 and a capsule tube 4;

the capsule ball 3 is enclosed into a capsule structure by a capsule wall 7, the top end of the capsule ball is provided with a capsule hole 5, the lower part of the capsule hole 5 is a capsule cavity 6, the upper half part of the capsule cavity 6 is a pressurizing cavity 6-1, the lower half part is a buffer cavity 6-2, and the lower end of the capsule ball 3 is tightly connected with a capsule pipe 4;

four punching pipe sleeves 8 with different specifications are sequentially connected and arranged in the capsule pipe 4 from top to bottom according to the hole diameters from small to large;

the punching pipe sleeves 8 can be respectively matched with four punching pipes 2 with different specifications;

the gel plate puncher of the embodiment is in a flower bud shape as a whole, the length of the gel plate puncher is 7 cm, and the outer diameter of the gel plate puncher is 2 cm.

The inner diameter of the capsule hole 5 is 2mm, the volume of a pressurizing cavity in the capsule cavity is 200 cubic millimeters, and the volume of a buffer cavity is 800 cubic millimeters.

The inner diameters of the first perforating pipe sleeve 8-1, the second perforating pipe sleeve 8-2, the third perforating pipe sleeve 8-3 and the fourth perforating pipe sleeve 8-4 are phi =2mm, 3mm, 4mm and 5mm respectively; the heights of the first punching pipe sleeve 8-1, the second punching pipe sleeve 8-2 and the third punching pipe sleeve 8-3 are all 5mm, and the height of the fourth punching pipe sleeve 8-4 at the lowest part is 10 mm.

The four perforated pipes 2 with different specifications are made of stainless steel pipes, 304 stainless steel can be adopted, the upper ends of the perforated pipes 2 are flat, and the lower ends of the perforated pipes are chamfered inwards along the outer diameter, so that the lower ends are sharp.

The first perforating pipe 2-1, the second perforating pipe 2-2, the third perforating pipe 2-3 and the fourth perforating pipe 2-4 have outer diameters phi =2mm, 3mm, 4mm and 5mm respectively, lengths are 50mm, 45mm, 40mm and 35mm respectively,

before the gel sheet 9 is punched, the necessary preparation is made by using the punch of the embodiment, for example, the gel sheet 9 is made, as shown in FIG. 3, a small beaker containing the gel block is prepared, and the gel sheet punch is assembled according to the experimental requirements. If aseptic operation is required, the gel plate puncher is packaged and autoclaved.

During punching, the punching operation is carried out according to the steps of embedding during zero pressure difference, pulling out during negative pressure difference and bouncing off during positive pressure difference. Placing the gel plate 9 on a table (gel is on the gel plate), setting the punching position of the gel plate, pinching the upper part of the punching tube 2 close to the capsule wall 7 by a thumb and a middle finger, leaving the capsule hole 5 by the index finger, communicating the pressurizing cavity 6-1, the buffer cavity 6-2 and the punching tube cavity with the outside, inserting the punching tube 2 into the gel plate at the moment when the pressure in the cavity is equal to the outside atmospheric pressure (namely 'zero pressure difference'), and embedding the gel into the punching tube 2. Then, the index finger belly lightly presses the capsule hole 5, at this time, the pressurizing cavity 6-1, the buffer cavity 6-2 and the punching tube cavity are isolated from the outside, the pressure intensity is still equal to the outside atmospheric pressure, at this time, the punching tube 5 is pulled out, the original shape can be kept due to the inertia of the gel block, when the punching tube 5 is pulled out by about half of the thickness of the gel, the volume of the punching tube cavity is increased, the pressure intensity is reduced, the formed negative pressure difference can suck the gel block, at this time, the punching tube 5 is pulled out, and the small beaker is moved. Then, the forefinger presses down the capsule wall 7, the volume of the pressurizing cavity 6-1 is compressed, the pressure is increased, a positive pressure difference is formed, the positive pressure difference is transmitted to the surface of the gel block in the perforated tube cavity, the gel block is ejected into the small beaker, and the gel plate hole 11 is punched. The holes are punched in this way, the edges of the holes are neat, no gap is left between the gel plate 9 and the glass slide 10, no leakage and no diffusion of sample liquid are ensured, and the experimental result is good, as shown in fig. 3.

The utility model discloses an application range is not only limited to the gel board, all can be applicable to the semi-solid offset plate (the thick 2-20mm of board) that many materials, for example materials such as agar, polyacrylamide made. The utility model discloses except being used for the immunoelectrophoresis experimental items, also can be used to the collection of microbial colony, projects such as fungi thallus separation inoculation.

Claims (4)

1. The utility model provides a gel plate hole puncher, includes capsule and the hole pipe of punching, its characterized in that:

the perforated capsule is divided into a capsule ball and a capsule tube;

the capsule ball is surrounded by the capsule wall to form a capsule structure, the top end of the capsule ball is provided with a capsule hole, the lower part of the capsule hole is a capsule cavity, the upper half part of the capsule cavity is a pressurizing cavity, the lower half part of the capsule cavity is a buffer cavity, and the lower end of the capsule ball is tightly connected with the capsule pipe;

four punching pipe sleeves with different specifications are sequentially connected in the capsule pipe from top to bottom according to the hole diameters from small to large;

the punching pipe sleeves can be matched with four punching pipes with different specifications respectively;

the gel plate puncher is in a flower bud shape as a whole;

the inner diameter of the capsule hole is 2mm, the volume of a pressurizing cavity in the capsule cavity is 200 cubic millimeters, and the volume of a buffer cavity is 800 cubic millimeters.

2. The gel-sheet punch of claim 1, wherein:

the inner diameters of the four punching pipe sleeves with different specifications are phi =2mm, 3mm, 4mm and 5mm respectively, the heights of the first punching pipe sleeve, the second punching pipe sleeve and the third punching pipe sleeve are 5mm, and the height of the fourth punching pipe sleeve at the bottom is 10 mm.

3. The gel-sheet punch of claim 1, wherein: the four perforated pipes with different specifications are made of stainless steel pipes, the outer diameters of the perforated pipes are phi =2mm, 3mm, 4mm and 5mm respectively, the lengths of the perforated pipes are 50mm, 45mm, 40mm and 35mm respectively, the upper ends of the perforated pipes are flat, and the lower ends of the perforated pipes are chamfered inwards along the outer diameters.

4. The gel-sheet punch of claim 1, wherein: the perforated capsule is a silica gel capsule.

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