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CN107475168A - 高起泡性志贺氏菌菌株 - Google Patents

高起泡性志贺氏菌菌株 Download PDF

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CN107475168A
CN107475168A CN201710653787.5A CN201710653787A CN107475168A CN 107475168 A CN107475168 A CN 107475168A CN 201710653787 A CN201710653787 A CN 201710653787A CN 107475168 A CN107475168 A CN 107475168A
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C.杰科
F.博兰达斯科萨
A.索尔
L.马格奥
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Global Health Gsk Vaccine Institute Inc
GSK Vaccines Institue for Global Health SRL
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Abstract

本发明涉及高起泡性志贺氏菌菌株。通过破坏Tol‑pal系统的一种或多种组分产生高起泡性志贺氏菌菌株。源自这些菌株的突泡是疫苗中有用的免疫原。在这些突泡中存在的单独的蛋白质也可用作免疫原。

Description

高起泡性志贺氏菌菌株
本申请是申请日为2010年9月28日的中国专利申请201080052643.0“高起泡性志贺氏菌菌株”的分案申请。
技术领域
本发明属于抗志贺氏菌的免疫法领域。
背景技术
志贺氏菌属是革兰氏阴性非运动性兼性厌氧菌，其可分为4种血清群：痢疾志贺氏菌(S.dysenteriae)、福氏志贺氏菌(S.flexneri)、鲍氏志贺氏菌(S.boydii)、宋内志贺氏菌(S.sonnei)。它们造成志贺氏菌痢(杆菌性痢疾)。
临床上志贺氏菌痢的标志是急性直肠结肠炎，其伴随有发烧、恶心、食欲减退、脱水、粘液脓性和血性腹泻以及里急后重。志贺氏菌导致的痢疾是地方病，可在发展中国家中导致成百万例的发病。例如，每年约有1.65亿例志贺氏菌痢，其中的99％发生在发展中国家，69％发生在5岁以下的儿童中。志贺氏菌痢造成的发病率和死亡率在发展中国家的儿童中尤其高。
参考文献1中对志贺氏菌疫苗的现有途径进行了综述，这些途径基于活减毒菌株，其用于口服免疫法、与O糖偶联用于注射、用于鼻内的蛋白体(连接有志贺氏菌LPS的脑膜炎球菌外膜囊泡)、用于鼻内的侵袭复合物(invaplexes，包含IpaB、IpaC和LPS的志贺氏菌属亚细胞提取物)、以及由具有去毒LPS的msbB-ve菌株制得的核蛋白-核糖体复合物。虽然这些疫苗中的一些已在实地试验中显效，但是没有一种疫苗可提供对抗多种志贺氏菌血清型的保护。
本发明的一个目的是提供在志贺氏菌疫苗制备中进一步提供和改进有用组分，尤其是可提供对抗多种志贺氏菌血清型的保护的疫苗。
发明内容
由于细胞被膜的膨压，志贺氏菌在生长期间自发地释放外膜突泡(bleb)。如参考文献2中所揭示，突泡的释放高度依赖于细菌外膜的结构。本发明人已经采用志贺氏菌的突变株来干扰外膜的结构，该突变株中的Tol-Pal系统已被破坏。在正常生长期间，这些突变株将大量突泡释放到其培养基中，这些突泡富含免疫原性外膜蛋白，因此这些突泡可被用作免疫原。
由此，本发明提供了表达TolA、TolB、TolQ、TolR和Pal蛋白中不多于4种蛋白质的志贺氏菌。因此，天然的五蛋白Tol-Pal系统中的至少一种蛋白质是缺失的，导致了细菌在培养基中的生长过程中，与表达全部五种Tol-Pal蛋白的相同细菌相比，将更多量的外膜突泡释放到培养基中。优选TolR不表达，但可表达其它四种蛋白质。
本发明还提供一种不表达TolR蛋白的志贺氏菌。本发明还提供一种志贺氏菌的ΔtolR株，例如ΔtolRΔgalU菌株。
本发明还提供一种志贺氏菌，其表达TolA、TolB、TolQ、TolR和Pal蛋白，其中所述TolA、TolQ、TolR和/或Pal蛋白(a)位于细菌的内膜或外膜内，且(b)包含一个或多个氨基酸序列突变，由此使得该细菌与不含所述突变的相同细菌相比，在培养基中生长时释放更大量的外膜突泡。
本发明还提供一种志贺氏菌，该细菌中的Tol-Pal系统中的一种或多种组分具有修饰，从而使得该细菌与缺乏修饰的相同细菌相比，在培养基中生长时将更大量的外膜突泡释放到培养基中，并且该细菌不表达：(i)天然志贺氏菌的脂多糖和/或(ii)志贺氏菌的肠毒素。
本发明还提供一种制备高起泡性志贺氏菌的方法，所述方法包括修饰编码起始细菌(starting bacterium)的Tol-Pal系统一种或多种组分的基因的步骤，所述修饰得到所述细菌与起始细菌相比，在培养基中生长时将更大量的外膜突泡释放到培养基中，且其中所述修饰涉及使得起始菌的tolA、tolB、tolQ、tolR和/或pal基因发生一种或多种突变。该突变步骤可为所述基因的缺失。该方法还可涉及编码合成该菌的脂多糖或肠毒素所需的蛋白质的基因的修饰。
本发明还提供一种分离或获自本发明的细菌的突泡。这些突泡可用作志贺氏菌疫苗的组分。
本发明还提供一种制备志贺氏菌突泡的方法，所述方法包括从包含本发明细菌的培养基中分离所述突泡的步骤，其中本发明细菌已在允许该细菌将突泡释放到所述培养基中的条件下生长。通过该方法制备的突泡可用作志贺氏菌疫苗的组分。
本发明还提供一种包含本发明细菌的培养基，所述细菌已在允许该细菌将突泡释放到所述培养基中的条件下生长。可从该培养基中纯化所述突泡。
本发明还提供一种包含突泡的组合物，所述突泡在本发明细菌的培养过程中释放入培养基。该组合物不含任何活的和/或完整细菌。该组合物和/或其组分可用于志贺氏菌疫苗的制备。
本发明还提供一种包含突泡的组合物，其中所述突泡存在于过滤液中，所述过滤液是将本发明细菌生长的培养基通过0.22μm滤器后获得的。该组合物和/或其组分可用于志贺氏菌疫苗的制备。
本发明还提供一种志贺氏菌突泡，其包含以下的一种或多种(即1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59或60种)：(a)由选自SEQ ID NO：8-67中的一种氨基酸序列组成的蛋白质；(b)包含与SEQ IDNO：8-67中的一种具有至少j％的相同性的氨基酸序列的蛋白质，其中j为50或更高(例如60、65、70、75、80、85、90、91、92、93、94、95、96、97、98、99)和/或包含SEQ ID NO：8-67中任一种中至少n个连续氨基酸的片段的蛋白质，其中n为7或更多(例如8、10、12、14、16、18、20、25、30、35、40、50、60、70、80、90、100、150、200、250或更多个)。优选的片段包含来自SEQ IDNO：8-67之一的抗原表位。其它优选的片段缺失所述SEQ ID NO：的C-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)和/或N-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)，但保留该SEQ ID NO：的至少一个表位。其它片段缺漏一个或多个蛋白质结构域，例如缺失信号肽等。
已确认本发明的突泡中存在60种蛋白质，这些蛋白质对于抗所述突泡制备的血清具有免疫反应性。因此，从突泡分离出的、以纯化形式存在的各种蛋白质可用作免疫原性组分。由此，本发明还提供包含突泡蛋白但不含突泡的免疫原性组合物，所述突泡蛋白包含：(a)氨基酸序列SEQ ID NO：8-67中的一种或多种(例如1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59或60种)；(b)与SEQ ID NO：8-67之一具有至少j％相同性的氨基酸序列，其中j为50或更高(例如60、65、70、75、80、85、90、91、92、93、94、95、96、97、98、99)和/或包含SEQ ID NO：8-67中任一种的至少n个连续氨基酸的片段，其中n为7或更多(例如8、10、12、14、16、18、20、25、30、35、40、50、60、70、80、90、100、150、200、250或更多)。优选的片段包含来自SEQ ID NO：8-67之一的抗原表位，更优选的片段为免疫原性片段。其它优选的片段缺失SEQ ID NO：的C-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)和/或N-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)，但保留该SEQ ID NO：的至少一个抗原表位。其它片段缺漏一个或多个蛋白结构域，例如缺失跨膜结构域、信号肽等。
本发明还提供一种志贺氏菌突泡，其包含以下的一种或多种(即1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128或129种)：(a)由选自SEQ ID NO：8-136中的一种氨基酸序列组成的蛋白质；(b)包含与SEQ ID NO：8-136中的一种具有至少j％的相同性的氨基酸序列的蛋白质，其中j为50或更高(例如60、65、70、75、80、85、90、91、92、93、94、95、96、97、98、99)和/或包含SEQID NO：8-136中任一种中至少n个连续氨基酸的片段的蛋白质，其中n为7或更多(例如8、10、12、14、16、18、20、25、30、35、40、50、60、70、80、90、100、150、200、250或更多个)。优选的片段包含来自SEQ ID NO：8-136之一的抗原表位。其它优选的片段缺失所述SEQ ID NO：的C-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)和/或N-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)，但保留该SEQ IDNO：的至少一个抗原表位。其它片段缺漏一个或多个蛋白质结构域，例如缺失信号肽等。
已确认本发明的突泡中存在129种蛋白质，这些蛋白质对于抗所述突泡制备的血清具有免疫反应性。因此，从突泡分离出的、以纯化形式存在的各种蛋白质可用作免疫原性组分。由此，本发明还提供包含突泡蛋白但不含突泡的免疫原性组合物，所述突泡蛋白包含：(a)氨基酸序列SEQ ID NO：8-136中的一种或多种(例如1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128或129种)；(b)与SEQ ID NO：8-136之一具有至少j％相同性的氨基酸序列，其中j为50或更高(例如60、65、70、75、80、85、90、91、92、93、94、95、96、97、98、99)和/或包含SEQ ID NO：8-136中任一种的至少n个连续氨基酸的片段的氨基酸序列，其中n为7或更多(例如8、10、12、14、16、18、20、25、30、35、40、50、60、70、80、90、100、150、200、250或更多)。优选的片段包含来自SEQ ID NO：8-136之一的抗原表位，更优选的片段为免疫原性片段。其它优选的片段缺失SEQ ID NO：的C-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)和/或N-末端的一个或多个氨基酸(例如1、2、3、4、5、6、7、8、9、10、15、20、25或更多个)，但保留该SEQ ID NO：的至少一个抗原表位。其它片段缺漏一个或多个蛋白结构域，例如缺失跨膜结构域、信号肽等。
在SEQ ID NO：8-136中，与福氏志贺氏菌相关的优选亚组是下表1的“亚组1”中所列的SEQ ID NO。在SEQ ID NO：8-136中，与宋内志贺氏菌相关的优选亚组是下表1的“亚组2”中所列的SEQ ID NO。
Tol-Pal系统
与许多革兰氏阴性菌类似，志贺氏菌天然具有由TolA、TolB、TolQ、TolR和Pal蛋白构成的Tol-Pal系统。根据本发明，天然Tol-Pal系统被破坏，从而导致细菌在细菌复制过程中将更大量的外膜突泡释放到其培养基中。可进行各种破坏。
在一些实施方式中，去除五种Tol-Pal蛋白中的至少一种，例如通过使得编码该蛋白的基因缺失或失活。因此，细菌可表达TolA、TolB、TolQ、TolR和Pal蛋白中的0、1、2、3或4种蛋白质。去除五种蛋白质中的一种可能已足够，在该情况下该细菌仅表达这些蛋白质中的四种。优选去除TolR蛋白，例如通过使得起始菌株的tolR基因失活。由此，该细胞可为tolA+tolB+tolQ+TolR-Pal+。
在一些实施方式中，该细菌表达全部五种Tol-Pal蛋白，但至少一种蛋白质发生突变而导致高起泡性。例如，TolA、TolQ、TolR和/或Pal蛋白可发生突变，从而使得蛋白质保留其膜定位，但其与其它Tol-Pal系统成员的相互作用被破坏。由此，该细菌将把作为跨膜蛋白的TolA、TolQ和TolR保留在内膜中，把作为向周质(periplasm-facing)脂蛋白的Pal蛋白保留在外膜中，但TolA、TolQ、TolR和/或Pal蛋白中的至少一种是突变的。
野生型志贺氏菌的TolA、TolB、TolQ、TolR和Pal蛋白的氨基酸序列的例子作为SEQID NO：1-5示于序列表中。
志贺氏菌
本发明可采用痢疾志贺氏菌(S.dysenteriae)、福氏志贺氏菌(S.flexneri)、鲍氏志贺氏菌(S.boydii)、宋内志贺氏菌(S.sonnei)的任何血清群。
除了具有被破坏的Tol-Pal系统而使得细菌在细菌复制期间将更大量的外膜突泡释放到其培养基中，本发明的志贺氏菌可有利地包含相对于野生型菌株而言的一种或多种其它改变。这些改变尤其可用于从该细菌中去除在人疫苗中可具有毒性或不为所需的组分。
例如，细菌可不表达天然志贺氏菌的脂多糖(LPS)，因此内毒素活性降低。可进行各种修饰以避免天然LPS的合成，这些修饰可破坏天然脂质A的结构、寡糖核心或外膜O抗原。例如，参考文献3报道了通过rfe和galU基因失活导致的LPS突变体，参考文献4报道了通过yihE、galE、galK、galM和galT失活导致的LPS突变体。类似地，参考文献5报道了因rfc、rfaL或galU突变导致的LPS缺陷。参考文献6报道了因msbBl和msbB2失活导致的LPS突变体，其减少了脂质A的酰化作用。如本文所示，可通过htrB的失活产生脂质A酰化减少的另一种LPS突变体[7，8]。
优选缺少LPS中的O抗原，从而避免血清型特异性的应答。在宋内志贺氏菌中，当毒性质粒被去除时，则缺少O抗原(见下文)。galU基因编码尿苷二磷酸葡萄糖(UDP-葡萄糖)焦磷酸化酶，该基因的失活导致所合成的LPS不含附着的(attached)O抗原。galU的失活有利于提供不具有尿苷二磷酸葡萄糖焦磷酸化酶活性的志贺氏菌。rfbF和/或rftG基因的失活可用于提供不具有鼠李糖基转移酶活性的志贺氏菌。rfc的失活可用于提供不具有O抗原聚合酶活性的志贺氏菌。rfbF、rfbG和rfc这三种基因都失活可提供有用的菌株。
优选缺少LPS中的六-酰化脂质A。失去毒性质粒(见下文)自动导致失去msbB2基因，染色体的msbBl基因可被失活，从而去除肉豆蔻酰基转移酶活性，并在LPS中提供五-酰化脂质A。HtrB月桂酰基转移酶的失活可提供主要含四-酰化脂质A的志贺氏菌。优选的菌株具有五-或四-酰化LPS。
优选菌株的galU和msbBl均失活，且还缺少毒性质粒，由此提供LPS为五-酰化且缺少附着的O抗原的菌株。
一些有用的菌株具有五-或四-酰化LPS，所述LPS包含附着的O抗原。然而更常规而言，优选菌株具有具有五-或四-酰化LPS，所述LPS缺少附着的O抗原。具有tolR、rfbG和htrB敲除(以及任选的rfbF和/或rfc失活)的福氏志贺氏菌菌株是有用的。一种有用的宋内志贺氏菌菌株具有tolR突变且缺少毒性质粒。
细菌可不表达肠毒素。例如，福氏志贺氏菌菌株(具体为2a菌株)可不表达全部的志贺氏菌肠毒素1(ShET-1)亚单位，例如可使得set1A和/或setlB基因失活。痢疾志贺氏菌菌株可不表达全部两种志贺氏菌毒素亚单位，例如可使得stxA和/或stxB基因中的一种或两种失活。志贺氏菌，尤其是宋内志贺氏菌或福氏志贺氏菌，可不表达肠毒素2(ShET-2)，例如可使得ospD3基因失活，或缺少毒性质粒。优选的菌株不编码ShET-1、ShET-2和志贺氏菌毒素。
可通过常规的诱变技术，从野生型或其它起始菌株方便地制备本发明的志贺氏菌，例如参见参考文献9-11。λRed重组系统(lambda red recombination system)是用于志贺氏菌的尤为有利的系统。可采用各种途径获得基因失活，例如通过其启动子的缺失或突变，通过其起始密码子的缺失或突变，通过导入提早形成终止密码子(premature stopcodon)，通过缺失整个编码区域，通过敲除等。优选等基因敲除突变体。在所得的志贺氏菌中，缺少编码所需基因的mRNA和/或其翻译被抑制(例如抑制为少于野生型水平的1％)。
本发明的志贺氏菌可包含代替失活基因的标记物基因，例如抗生素抗性标记物。其可通过同源重组完成。然而优选使用未标记的缺失(即不引入标记物基因的缺失)。
毒性志贺氏菌菌株具有220kb的质粒，该质粒介导毒性性质。已显示该″毒性质粒″编码涉及志贺氏菌毒性的数个方面的基因，这些方面包括用于靶向上皮细胞的细菌细胞表面配基、侵袭质粒抗原、virF、virG等。本发明的志贺氏菌可具有毒性质粒，但优选其不具有毒性质粒。缺少该质粒可使得菌株在工业化培养中稳定、通过去除毒性因素而减毒(从而提高生产安全性)、破坏脂多糖(O抗原的生物合成基因位于志贺氏菌中的质粒上)、避免存在ShET-2肠毒素(由质粒上的ospD3或sen基因编码)，以及避免存在msbB2，该基因是负责脂质A酰化的msbB基因的第二拷贝。
本发明的志贺氏菌可表达一种或多种异源蛋白，例如志贺氏菌中天然不存在的蛋白质。如果该异源蛋白是外膜蛋白，则来自该菌株的突泡可用作将非志贺氏菌抗原递呈到免疫系统的递送系统。
使志贺氏菌生长的培养条件是本领域中熟知的，例如参见参考文献12-14。例如，它们的培养可采用有机氮源(例如氨基酸混合物，如包含Ala、Arg、Asn、Asp；可使用酪蛋白氨基酸)，以丙三醇为碳源等等。在培养基中加入L-天冬氨酸尤为有利，其可同时作为氮源和碳源。
可在限铁(iron-limiting)条件下有利地培养本发明的志贺氏菌，已显示这些条件可上调志贺氏菌属中具有免疫原性且高度保守的铁调蛋白。例如，可在诸如去铁灵(desferal)或2，2′-联吡啶基或8-羟基喹啉的化合物存在下培养细菌。在这些条件下，细菌可提高蛋白质(例如FepA外膜受体、大肠杆菌素I受体(CirA)和/或铁嗜铁素受体(FhuA))的表达。
突泡和高起泡性
本发明的志贺氏菌与其相应的野生型菌株相比，具有高起泡性，即它们把与野生型菌株相比更大量的突泡释放到其培养基中。这些突泡可用作志贺氏菌疫苗的组分。
由电子显微镜测得的突泡直径通常为35-120nm，例如50nm的直径。
突泡在细菌培养过程中自发释放，并可从该培养基中纯化。理想的纯化包括从活的和/或完整的志贺氏菌中分离突泡，例如通过采用过滤器(例如0.22μm的过滤器)的基于大小的过滤，该过滤器允许突泡通过但不允许完整细菌通过，或通过使用低速离心使细胞沉淀而将突泡留在悬液中。
因此，与培养基不同，本发明的含突泡组合物通常基本上不含活的或死的全菌。突泡的大小意味着它们可通过过滤(例如象通常用于过滤灭菌的过滤)简便地与全菌分离。虽然突泡可滤过标准的0.22μm过滤器，它们会很快被其它物质阻塞，因此在使用0.22μm过滤器之前进行滤过一系列具有降低的孔径的过滤器的过滤除菌循序步骤是有利的。之前过滤器的例子包括具有0.8μm、0.45μm等孔径的过滤器。
从培养基中分离自发释放的突泡比涉及故意破坏外膜(例如通过去污剂除了处理或超声作用)以形成外膜小囊泡的方法更为简便。此外，它们基本上不含内膜和细胞质污染物。
本发明的突泡包含脂质和蛋白质。已分析了突泡的蛋白质内容物，它们包括表1中所列和下文所讨论的蛋白质。
志贺氏菌外膜蛋白
表1列出了本发明的志贺氏菌突泡中检测到的129种蛋白质的GenBank名称和GI号。这127种蛋白质可从突泡中分离、以纯化的形式用作免疫原性成分。这129种蛋白质中的优选亚组是表中的前60种(“亚组3”)。
可通过多种方式制备多肽，例如通过化学合成(至少部分合成)，通过用蛋白酶消化较长的多肽，通过RNA翻译，通过从细胞培养物纯化(例如从重组表达或从志贺氏菌培养物)等等。在大肠杆菌宿主中进行异源表达是优选的表达途径。
本发明的多肽可附着于或固定于固相载体。本发明的多肽可包含可检测标记物，例如放射性标记物、荧光标记物、或生物素标记物。这在免疫分析技术中尤为有用。
多肽可以各种形式存在(例如天然的、融合的、糖基化的、非糖基化的、脂化的、具有二硫桥等等)。多肽优选为志贺氏菌多肽。
优选以基本上纯或基本上分离的形式(即基本上不含其它志贺氏菌或宿主细胞多肽)或基本上分离的形式制备多肽。总体而言，所提供的多肽处于非自然存在环境，例如它们从其天然存在环境中分离。在某些实施方式中，对象肽存在于与对照相比富含该多肽的组合物中。由此，提供了纯化的多肽，其中“纯化的”是指该多肽存在于基本上不含其它表达的多肽的组合物中，且其中“基本上不含”是指该组合物的少于50％，通常少于30％，更通常少于10％是由其它表达的多肽构成的。
术语“多肽”是指任何长度的氨基酸聚合物。该聚合物可为线性或分支的，其可包含修饰的氨基酸，且其可被插入有非氨基酸。该术语还包括已被天然修饰或被干扰的氨基酸聚合物；例如，二硫键形成、糖基化、脂化、酰化、磷酸化或其它操作或修饰，例如与标记组分偶联。在该定义中还包括例如，含一个或多个氨基酸类似物(包括例如，非天然氨基酸等)以及本领域中已知的其它修饰的多肽。多肽可作为单链存在或作为相结合的链存在。
药物组合物
本发明提供了一种药物组合物，其包含：(a)本发明的突泡；以及(b)药学上可接受的载体。本发明还提供一种制备该组合物的方法，所述方法包括将本发明的突泡与药学上可接受的载体混合的步骤。
本发明还提供了一种药物组合物，其包含：(a)如上所定义的不含突泡的免疫原性组合物；以及(b)药学上可接受的载体。
免疫原性组合物可包含药学上可接受的载体，所述载体可为本身不会诱导产生对接受该组合物的患者有害的抗体的任何物质，且在给予时不会产生过度的毒性。药学上可接受的载体可包括液体，例如水、盐水、丙三醇和乙醇。在此类载体中还可存在辅助物质，例如润湿剂或乳化剂，pH缓冲物质等。关于适合的载体的全面讨论可参见参考文献15。
志贺氏菌感染可影响身体的各种区域，因此可将本发明的组合物制成各种形式。例如，可将该组合物制成作为液态溶液或悬浮液的可注射形式。还可制备适于在注射前溶于或悬浮于液体载剂中的固体形式。可将该组合物制成用于局部给药，例如制成软膏、乳膏或粉末。可将该组合物制成用于口服给药，例如制成片剂或胶囊，或制成糖浆(任选经调味)。可将该组合物制成用于肺部给药，例如制成吸入剂，使用细粉或喷雾器。可将该组合物制成栓剂或阴道栓。可将该组合物制成用于经鼻、经耳或经眼给药，例如制成滴剂。
优选该组合物是无菌的。优选其无热原。优选其经缓冲，例如pH为6-8，通常为pH7左右。用于人时，本发明的组合物可为等渗组合物。
免疫原性组合物包含免疫有效量的免疫原，以及所需的任何其它特定组分。“免疫有效量”是指将该量以单剂量或系列中的部分给予个体可有效治疗或预防。该量的变化取决于待治疗个体的健康和生理状况、年龄、待治疗个体的分类群(例如非人灵长类，灵长类等)、个体免疫系统对综合抗体的容量、所需的保护程度、疫苗的制剂、治疗医师对医疗情况的评估、以及其它相关因素。预期该量的范围较宽，该范围可通过常规试验确定。
采用小囊泡疫苗(例如用于脑膜炎球菌)的早先工作为突泡给药提供了药学、剂量学和制剂指导。本发明组合物中突泡的浓度通常为10-500μg/ml，优选25-200μg/ml，更优选约50μg/ml或约100μg/ml(就突泡中的总蛋白而言)。用于注射的剂量体积通常为0.5ml。
该组合物可与其它免疫调节剂联合给药。
可用于本发明组合物(尤其是无突泡组合物)中的佐剂包括但不限于：
A.含无机物组合物
适于用作本发明中的佐剂的含无机物组合物包括：无机盐，例如铝盐和钙盐。本发明包括无机盐，例如氢氧化物(如氢氧化合物)、磷酸盐(例如羟基磷酸盐，正磷酸盐)、硫酸盐等[例如参见参考文献19的第8和9章]，或不同无机化合物的混合物，其中化合物可为任何形式(例如凝胶、结晶、无定形等)，优选吸附。还可将含无机物组合物配制为金属盐颗粒。
被称为“氢氧化铝”的佐剂是常规的氢氧化铝氧化物的盐，其通常至少部分结晶化。氢氧化铝氧化物(可由式AlO(OH)表示)可通过红外(IR)光谱与其它铝化合物(例如氢氧化铝Al(OH)3)区分开，尤其可通过1070cm-1处的吸收带和3090-3100cm-1处的强肩峰的存在来区分[参考文献19第9章]。半峰高处的衍射带宽度(WHH)反映出氢氧化铝佐剂的结晶度，结晶差的颗粒由于具有较小的微晶尺寸而显示出较大的谱线增宽。当WHH增加，表面积增加，具有较高WHH值的佐剂已显示出具有较大的抗原吸附容量。氢氧化铝佐剂通常呈纤维形态(fibrous morphology，例如在透射电子显微照片中所见)。氢氧化铝佐剂的pI通常为11，即在生理pH下，该佐剂本身具有表面正电荷。已报道氢氧化铝佐剂在pH7.4时的吸附容量为1.8-2.6mg蛋白质/mgAl+++。
称为“磷酸铝”的佐剂通常是羟基磷酸铝，常还含有少量硫酸盐(即羟基磷酸硫酸铝)。其可通过沉淀获得，沉淀期间的反应条件和浓度影响磷酸根取代所述盐中羟基的程度。羟基磷酸盐中PO4/Al摩尔比通常在0.3-1.2之间。羟基磷酸盐因存在羟基而有别于严格的AlPO4。例如，3164cm-1处的IR谱带(例如在200℃)表明结构性羟基的存在[参考文献19第9章]。
磷酸铝佐剂的PO4/Al3+摩尔比通常为0.3-1.2，优选为0.8-1.2，更优选为0.95±0.1。磷酸铝通常是无定形的，尤其是羟基磷酸盐。典型的佐剂是PO4/Al摩尔比为0.84-0.92的无定形的羟基磷酸铝，包含0.6mg Al3+/ml。磷酸铝通常是颗粒状(如在透射电子显微照片上观察到的片状形态)。在任何抗原吸附后，颗粒的典型直径为0.5-20μm(例如约5-10μm)。已报道氢氧化铝佐剂在pH7.4时的吸附容量为0.7-1.5mg蛋白质/mgAl+++。
磷酸铝的零电点(PZC)与磷酸根取代羟基的程度逆相关，这种取代程度可根据用于通过沉淀制备盐的反应条件和反应物浓度而变化。也通过改变溶液中游离磷酸根离子的浓度(更多磷酸根＝更酸性的PZC)或加入缓冲剂(如组氨酸缓冲剂)(使PZC碱性更强)改变PZC。本发明所用的磷酸铝的PZC通常为4.0-7.0，更优选为5.0-6.5，例如约为5.7。
用于制备本发明组合物的铝盐悬浮液可以含有缓冲液(如磷酸盐或组氨酸或Tris缓冲液)，但这并不一直是必需的。该悬浮液优选无菌且无热原。悬浮液可含有游离的水性磷酸根离子，如存在浓度为1.0-20mM，优选5-15mM，更优选约10mM。该悬浮液也可含有氯化钠。
在一个实施方式中，佐剂组分包括氢氧化铝和磷酸铝的混合物。在该情况下，磷酸铝的量多于氢氧化铝，例如重量比至少为2∶1，例如≥5∶1、≥6∶1、≥7∶1、≥8∶1、≥9∶1等。
给予患者的组合物中Al+++的浓度优选小于10mg/ml，例如，≤5mg/ml、≤4mg/ml、≤3mg/ml、≤2mg/ml、≤1mg/ml等。优选范围是0.3-1mg/ml。优选最大值是＜0.85mg/剂。
B.油乳剂
适用作本发明的佐剂的油乳剂组合物包括鲨烯-水乳剂，例如MF59[参考文献19的第10章；还可参见参考文献16](5％鲨烯、0.5％吐温80、和0.5％司盘85，用微流化床配制成亚微米颗粒)。也可以使用完全弗氏佐剂(CFA)和不完全弗氏佐剂(IFA)。
已知各种适合的水包油乳剂，它们通常包括至少一种油和至少一种表面活性剂，所述油和表面活性剂是可生物降解(可代谢)和生物相容的。乳剂中油滴的直径通常小于5μm，优选乳剂包含具有亚微米直径的油滴，通过微流化床实现这种小尺寸以提供稳定乳剂。优选尺寸小于220nm的液滴，因为可对其进行过滤灭菌。
本发明可使用油，例如来自动物(如鱼)或植物来源的油。植物油的来源包括坚果、种籽和谷物。最常用的花生油、大豆油、椰子油和橄榄油是坚果油的示例。也可使用获自(例如)霍霍巴豆的霍霍巴油。种籽油包括红花油、棉花籽油、葵花籽油、芝麻籽油等。在谷物类中，最常用的是玉米油，但也可以使用其它谷类的油，如小麦、燕麦、黑麦、稻、画眉草、黑小麦等。可从坚果和种籽油开始，通过水解、分离和酯化合适物质制备甘油和1，2-丙二醇的6-10碳脂肪酸酯，其不是种籽油中天然存在。来自哺乳动物乳汁的脂肪和油类是可代谢的，因此可以用于实施本发明。获得动物来源纯油所必需的分离、纯化、皂化和其它方法的过程为本领域熟知。大多数鱼类含有容易回收的可代谢油。例如，鳕鱼肝油、鲨鱼肝油和鲸油(例如鲸蜡)是可以用于本发明的几种鱼油的示例。通过生化途径用5-碳异戊二烯单位合成许多支链油，其总称为萜类。鲨鱼肝油含有称为鲨烯的支链不饱和萜类化合物，即2，6，10，15，19，23-六甲基-2，6，10，14，18，22-二十四碳六烯。其它优选油是生育酚(见下文)。包含鲨烯的水包油乳剂是特别优选的。可以使用油的混合物。
可通过′HLB′(亲水/亲脂平衡)对表面活性剂分类。本发明优选的表面活性剂的HLB为至少10，优选至少15，更优选至少16。本发明可与表面活性剂一起使用，所述表面活性剂包括但不限于：聚氧乙烯去水山梨糖醇酯表面活性剂(通常称为吐温)，特别是聚山梨酯20和聚山梨酯80；以商品名DOWFAXTM出售的环氧乙烷(EO)、环氧丙烷(PO)和/或环氧丁烷(BO)的共聚物，如直链EP/PO嵌段共聚物；重复的乙氧基(氧-1，2-乙二基)数量不同的辛苯聚醇，特别感兴趣的是辛苯聚醇9(曲通(Triton)X-100，或叔辛基苯氧基聚乙氧基乙醇)；(辛基苯氧基)聚乙氧基乙醇(IGEPAL CA-630/NP-40)；磷脂，如磷脂酰胆碱(卵磷脂)；衍生自十二烷醇、十六烷醇、十八烷醇和油醇的聚氧乙烯脂肪醚(称为苄泽(Brij)表面活性剂)，如三乙二醇单月桂基醚(苄泽30)；以及去水山梨糖醇酯(总称为司盘(Span))，如去水山梨糖醇三油酸酯(司盘85)和去水山梨糖醇单月桂酸酯。乳剂中包含的优选表面活性剂是吐温80(聚氧乙烯脱水山梨糖醇单油酸酯)、司盘85(脱水山梨糖醇三油酸酯)、卵磷脂和曲通X-100。如上所述，吐温80等去污剂可提供下文实施例所见的热稳定性。
可使用表面活性剂的混合物，如吐温80/司盘85混合物。聚氧乙烯去水山梨糖醇酯(如聚氧乙烯去水山梨糖醇单油酸酯，吐温80)和辛苯聚醇(如叔辛基苯氧基聚乙氧基乙醇，曲通X-100)的组合也适合。另一种有用的组合包含月桂醇聚醚-9(laureth 9)加聚氧乙烯去水山梨糖醇酯和/或辛苯聚醇。
表面活性剂的优选量(重量％)为：聚氧乙烯去水山梨糖醇酯(如吐温80)0.01-1％，特别是约0.1％；辛基-或壬基-苯氧基聚氧乙醇(如曲通X-100或曲通系列的其它去污剂)0.001-0.1％，特别是0.005-0.02％；聚氧乙烯醚(如月桂醇聚醚9)0.1-20％，优选0.1-10％，特别是0.1-1％或约0.5％。
可用于本发明的特定水包油乳剂佐剂包括但不限于：
●鲨烯、吐温80和司盘85的亚微米乳剂。所述乳剂的体积组成可以是约5％鲨烯、约0.5％聚山梨酯80和约0.5％司盘85。以重量计，这些比例为4.3％鲨烯、0.5％聚山梨酯80和0.48％司盘85。这种佐剂称为‘MF59’[16-18]，参考文献19的第10章和参考文献20的第12章更详细地描述了该佐剂。MF59乳剂优选包含柠檬酸根离子，如10mM柠檬酸钠缓冲液。
●包含鲨烯、α-生育酚和聚山梨酯80的乳剂。这些乳剂可含有2-10％鲨烯、2-10％生育酚和0.3-3％吐温80，鲨烯：生育酚的重量比优选≤1(例如0.90)，因为这能使乳剂更稳定。鲨烯和吐温80的体积比可以约为5∶2，或者重量比约为11∶5。可通过下述方法制备一种这样的乳剂：将吐温80溶解于PBS得到2％溶液，然后将90ml该溶液与(5g DL-α-生育酚和5ml鲨烯)的混合物混合，然后使该混合物微流体化。所得乳剂可含有亚微米油滴，如平均直径为100-250nm，优选约180nm的亚微米油滴。
●鲨烯、生育酚和曲通去污剂(如曲通X-100)的乳剂。所述乳剂还可包含3d-MPL(见下文)。所述乳剂可包含磷酸盐缓冲液。
●含有聚山梨酯(如聚山梨酯80)、曲通去污剂(如曲通X-100)和生育酚(如琥珀酸α-生育酚)的乳剂。所述乳剂可包含比例为75∶11∶10(例如750μg/ml聚山梨酯80，110μg/ml曲通X-100和100μg/ml的琥珀酸α-生育酚)的这三种成分，且这些浓度可包含由抗原中的这些组分做出的任何贡献。所述乳剂还可包含鲨烯。所述乳剂还可包含3d-MPL(见下文)。水相可包含磷酸盐缓冲液。
●鲨烯、聚山梨酯80和泊洛沙姆401(“普朗尼克(Pluronic)TML121”)的乳剂。所述乳剂可用pH 7.4的磷酸盐缓冲盐水配制。该乳剂是一种有用的胞壁酰二肽递送载体，且已与含苏氨酰基-MDP一起在“SAF-1”佐剂中使用[21](0.05-1％Thr-MDP、5％鲨烷、2.5％普朗尼克L121和0.2％聚山梨酸酯80)。也可不与Thr-MDP一起使用，如在“AF”佐剂中[22](5％鲨烯、1.25％普朗尼克L121和0.2％聚山梨酸酯80)。优选进行微流体化。
●含有鲨烯、水性溶剂、聚氧乙烯烷基醚亲水性非离子表面活性剂(例如聚氧乙烯(12)鲸蜡硬脂醚)和疏水性非离子表面活性剂(例如脱水山梨糖醇酯或二缩甘露醇酯，例如去水山梨糖醇单油酸酯或“司盘80”)。乳剂优选为热可逆的和/或至少90％的油滴(以体积计)的尺寸小于200nm[23]。乳剂还可包含一种或多种如下物质：醛糖醇；低温防护剂(例如糖，如十二烷基麦芽糖苷和/或蔗糖)；和/或烷基聚糖苷。可冷冻干燥该乳剂。
●含有0.5～50％油、0.1～10％磷脂和0.05～5％非离子表面活性剂的乳剂。如参考文献24所述，优选的磷脂组分是：磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸、磷脂酰肌醇、磷脂酰甘油、磷脂酸、鞘磷脂和心肌磷脂。亚微米的微滴尺寸是有利的。
●不可代谢的油(例如轻质矿物油)和至少一种表面活性剂(例如卵磷脂、吐温80或司盘80)的亚微米水包油乳剂。可加入添加剂，例如皂树A皂甙(QuilA saponin)、胆固醇、皂甙-亲脂性偶合物(例如GPI-0100，描述于参考文献25，通过葡萄糖醛酸的羧基将脂族胺添加到脱乙酰基皂甙而产生)、溴化二甲基双十八烷基铵和/或N，N-双十八烷基-N，N-二(2-羟乙基)丙二胺。
●包含矿物油、非离子亲脂性乙氧基化脂肪醇、非离子亲水表面活性剂(例如乙氧基化脂肪醇和/或聚氧乙烯-聚氧丙烯嵌段共聚物)的乳剂[26]。
●包含矿物油、非离子亲水性乙氧基化脂肪醇、和非离子亲脂性表面活性剂(例如乙氧基化脂肪醇和/或聚氧乙烯-聚氧丙烯嵌段共聚物)的乳剂[26].
●所含皂甙(例如QuilA或QS21)和固醇(例如胆固醇)结合成螺旋状胶束的乳剂[27]。
通常，在递送给病人时，组合物中的抗原和佐剂是混合的。乳剂可以在生产过程中与抗原混合，或是在递送时将该乳剂与抗原临时混合。因此，在包装或出售的疫苗中，该佐剂和抗原可分开保存，使用时配制成最终制剂。所述抗原通常是水性形式，从而最终通过混合两种液体制备疫苗。用于混合的两种液体的体积比是可变的(例如5∶1至1∶5)，但通常约为1∶1。
C.皂苷制剂[参考文献19的第22章]
皂苷制剂也可以用作本发明的佐剂。皂苷是在许多种类植物的树皮、叶、茎干、根/甚至花中发现的甾醇糖苷和三萜葡萄糖苷的异质群体。已广泛研究了作为佐剂的得自皂皮树(Quillaia saponaria Molina)树皮的皂苷。皂苷也可商业化获自丽花菝葜(Smilaxornata)(墨西哥菝葜)、满天星(Gypsophilla paniculata)(婚纱花)和肥皂草(Saponariaofficianalis)(皂根)。皂苷佐剂制剂包括纯化的制剂，如QS21，以及脂质制剂，如ISCOM。QS21以商标StimulonTM出售。
已采用HPLC和RP-HPLC纯化皂苷组合物。已鉴定了用这些技术纯化的特定组分，包括QS7、QS17、QS18、QS21、QH-A、QH-B和QH-C。所述皂苷优选为QS21。制备QS21的方法参见参考文献28。皂苷制剂也可包含甾醇，如胆固醇[29]。
皂苷和胆固醇的组合可用于形成称为免疫刺激复合物(ISCOM)的独特颗粒(参见参考文献19的第23章；也可参见参考文献30和31)。ISCOM通常还包含磷脂，如磷脂酰乙醇胺或磷脂酰胆碱。任何已知的皂苷均可用于ISCOM中。ISCOM优选包含QuilA、QHA和QHC中的一种或多种。任选地，ISCOM可不含其它去污剂[32]。
关于基于皂苷的佐剂开发的综述可参见参考文献33和34。
D.细菌或微生物衍生物
适用于本发明的佐剂包括细菌或微生物衍生物，例如肠细菌脂多糖(LPS)的无毒衍生物、脂质A衍生物、免疫刺激性寡核苷酸和ADP-核糖基化毒素及其脱毒衍生物。
LPS的无毒衍生物包括单磷酰脂质A(MPL)和3-O-脱酰基MPL(3dMPL)。3dMPL是3脱-O-酰基单磷酰脂质A与4、5或6条酰化链的混合物。3脱-O-酰基单磷酰脂质A的优选“小颗粒”形式见参考文献35中所述。3dMPL的此类“小颗粒”小到足以在过滤除菌时通过0.22μm的膜[35]。其它无毒LPS衍生物包括单磷酰脂质A模拟物，如氨基烷基氨基葡萄糖苷磷酸盐衍生物，例如RC-529[36，37]。
脂质A衍生物包括大肠杆菌的脂质A衍生物，如OM-174。例如参考文献38和39中描述了OM-174。
适合用作本发明佐剂的免疫刺激性寡核苷酸包括含CpG基序的核苷酸序列(含有通过磷酸键与鸟苷连接的非甲基化胞嘧啶的二核苷酸序列)。含回文结构或聚(dG)序列的双链RNA和寡核苷酸也显示具有免疫刺激作用。
CpG可包含核苷酸修饰/类似物，如硫代磷酸酯修饰，可以是双链或单链。参考文献40、41和42公开了可能的类似取代，例如用2′-脱氧-7-脱氮鸟苷取代鸟苷。参考文献43-48中进一步讨论了CpG寡核苷酸的佐剂作用。
CpG序列可能导向TLR9，例如基序GTCGTT或TTCGTT[49]。CpG序列可特异性诱导Th1免疫应答，例如CpG-A ODN，或更特异地诱导B细胞应答，例如CpG-B ODN。参考文献50-52中讨论了CpG-A和CpG-B ODN。优选CpG为CpG-A ODN。
优选构建CpG寡核苷酸，从而使得5′末端易被受体识别。任选使两个CpG寡核苷酸序列在3′末端连接以形成“免疫聚体(immunomer)”。参见，例如参考文献53-55。
基于免疫刺激性寡核苷酸的特别有用的佐剂被称为IC-31TM[56-58]。因此，本发明使用的佐剂可以包含(i)和(ii)的混合物：(i)含有至少一个(优选多个)CpI基序(即胞嘧啶与肌苷相连形成二核苷酸)的寡核苷酸(例如15-40个核苷酸)，和(ii)聚阳离子聚合物，如含有至少一个(优选多个)Lys-Arg-Lys三肽序列的寡肽(如5-20个氨基酸)。所述寡核苷酸可以是包含26-聚体序列5′-(IC)13-3′(SEQ ID NO：7)的脱氧核苷酸。聚阳离子聚合物可以是含有11-聚体氨基酸序列KLKLLLLLKLK的肽(SEQ ID NO：6)。SEQ ID NO：6和7的组合提供了IC-31TM佐剂。
细菌ADP-核糖基化毒素及其去毒衍生物可以用作本发明的佐剂。优选所述蛋白质衍生自大肠杆菌(大肠杆菌不耐热肠毒素“LT”)、霍乱菌(“CT”)或百日咳菌(“PT”)。参考文献59中描述了将脱毒的ADP-核糖基化毒素用作粘膜佐剂，参考文献60中描述了将其用作胃肠道外佐剂。所述毒素和类毒素优选全毒素形式，包含A和B亚单位。A亚单位优选含有脱毒突变；B亚单位优选不突变。所述佐剂优选为脱毒的LT突变体，如LT-K63、LT-R72和LT-G192。参考文献61-68中描述了将ADP-核糖基化毒素及其脱毒衍生物，尤其是LT-K63和LT-R72用作佐剂。一种有用的CT突变体是CT-E29H[69]。氨基酸取代的编号优选基于参考文献70中提出的ADP-核糖基化毒素的A和B亚单位的比对，该参考文献的全部内容特别纳入本文作为参考
E.人免疫调节剂
适合用作本发明佐剂的人免疫调节剂包括细胞因子，如白介素(例如，IL-1、IL-2、IL-4、IL-5、IL-6、IL-7、IL-12[71]等)[72]、干扰素(例如干扰素-γ)、巨噬细胞集落刺激因子和肿瘤坏死因子。优选的免疫调节剂是IL-12。
F.生物粘着剂和粘膜粘着剂
生物粘着剂和粘膜粘着剂也可以用作本发明的佐剂。合适的生物粘着剂包括酯化透明质酸微球[73]或粘膜粘着剂，如聚乙烯醇、聚乙烯吡咯烷酮、多糖和羧甲基纤维素、聚(丙烯酸)的交联衍生物。壳聚糖及其衍生物也可用作本发明的佐剂[74]。
G.微粒
微粒也可以用作本发明的佐剂。微粒(即直径为～100nm至～150μm，更优选直径～200nm至～30μm，最优选直径～500nm至～10μm的颗粒)由生物可降解的无毒材料(例如，聚(α-羟酸)、聚羟基丁酸、聚原酸酯、聚酐、聚己内酯等)形成，优选聚丙交酯乙交酯共聚物，并任选经处理而具有带负电表面(例如用SDS处理)或带正电表面(例如用阳离子去污剂如CTAB处理)。
H.脂质体(参考文献19的第13和14章)
适于用作佐剂的脂质体制剂的例子见参考文献75-77所述。
I.咪唑并喹诺酮(Imidazoquinolone)化合物
适合用作本发明佐剂的咪唑并喹诺酮化合物的例子包括咪喹莫特及其同系物(例如，″瑞喹莫德3M″)，见参考文献78和79所述。
本发明也可包含以上说明的一种或多种佐剂各方面的组合。例如，可将以下佐剂组合物用于本发明：(1)皂苷和水包油乳剂[80]；(2)皂苷(如QS21)+无毒LPS衍生物(如3dMPL)[81]；(3)皂苷(如QS21)+无毒LPS衍生物(如3dMPL)+胆固醇；(4)皂苷(如QS21)+3dMPL+IL12(任选+固醇)[82]；(5)3dMPL与(例如)QS21和/或水包油乳剂的组合[83]；(6)SAF，含有10％鲨烯、0.4％吐温80TM、5％普朗尼克-嵌段聚合物L121和thr-MDP，微流体化成为亚微米乳剂或涡旋振荡产生粒度较大的乳剂；(7)RibiTM佐剂系统(RAS)(瑞比免疫化学公司(Ribi Immunochem))，含有2％鲨烯、0.2％吐温80和一种或多种细菌细胞壁组分，所述组分选自单磷酰脂质A(MPL)、海藻糖二霉菌酸酯(TDM)或细胞壁骨架(CWS)，优选MPL+CWS(DetoxTM)；和(8)一种或多种无机盐(如铝盐)+LPS的无毒衍生物(如3dMPL)。
用作免疫刺激剂的其它物质可参见参考文献19的第7章。
可用氢氧化铝佐剂，抗原通常吸附在该盐上。还优选包含鲨烯的水包油乳液，其具有亚微米油滴，尤其是在年长者中。有用的佐剂组合包括Th1和Th2佐剂的组合，如CpG和铝盐，或瑞喹莫德和铝盐。可使用铝盐和3dMPL的组合。
免疫法
除提供上述免疫原性组合物外，本发明还提供一种在哺乳动物中引起抗体应答的方法，包括将本发明的免疫原性组合物给予所述哺乳动物。所述抗体应答优选地为保护性抗体应答。本发明还提供用于这类方法中的本发明组合物。
本发明还提供了一种保护哺乳动物免受志贺氏菌感染和/或疾病(例如志贺氏菌痢、赖特综合征和/或溶血性尿毒症综合征)的方法，包含将本发明中的免疫原性组合物给予所述哺乳动物。
本发明提供用作药物(例如，用作免疫原性组合物或疫苗)的本发明组合物。本发明还提供本发明的囊泡在制备防止哺乳动物中志贺氏菌感染的药物中的应用，例如用于预防志贺氏菌痢、赖特综合征和/或溶血性尿毒症综合征。还提供本发明的突泡蛋白(如前所述)在制备防止哺乳动物中志贺氏菌感染的无突泡药物中的应用，例如用于预防志贺氏菌痢。
哺乳动物优选为人。所述人可以是成人，或优选是儿童。当预防性使用疫苗时，人优选为儿童(如幼童或婴儿)；当疫苗用于治疗用途时，人优选为成人。准备给予儿童的疫苗也可给予成年人，例如用以评估安全性、剂量、免疫原性等。
所述用途和方法特别适用于预防/治疗疾病，这些疾病包括但并不限于：志贺氏菌痢、赖特综合征和/或溶血性尿毒症综合征。
治疗性措施的功效可以通过在给予本发明中的组合物后对志贺氏菌感染进行监测来测试。可通过监测在施用组合物后针对突泡中免疫原性蛋白或其它抗原的免疫应答来测试预防性治疗的效力。本发明组合物的免疫原性可通过以下方法测定：将其给予受试对象(如12-16个月大的儿童)，然后测定标准血清学参数。这些免疫应答通常在给予该组合物后约4周测定，并与给予该组合物之前测定的值作比较。当给予一剂以上的组合物时，可以进行一次以上的给药后测定。
本发明的组合物通常直接给予患者。可以通过胃肠外注射(例如，皮下、腹膜内、静脉内、肌肉内或给予到组织间隙)，或通过直肠、口服、阴道、局部、透皮、鼻内、眼部、耳部、肺部或其它粘膜给药途径完成直接递送。优选在大腿或上臂进行肌肉内给药。注射可以通过针头(例如皮下针头)进行，但也可以采用无针注射。通常的肌肉内剂量约为0.5ml。
本发明可用来引发全身和/或粘膜免疫。
剂量治疗可采用单剂量方案或多剂量方案。多剂量可以用于初次免疫方案和/或加强免疫方案。初次给药方案之后，可以进行加强给药方案。可以常规地确定初次剂量之间(例如4-16周)以及初次和加强剂量之间的适当时机。
概述
术语“包含”涵盖“含有”以及“由……组成”，例如“包含”X的组合物可仅由X组成或可含有其它物质，例如X+Y。
与数值x相关的术语“约”是任选的，其表示(例如)x±10％。
词语“基本上”不排除“完全”，如“基本上不含”Y的组合物可能完全不含Y。在必要时，词语“基本上”可从本发明的定义中省略。
两条氨基酸序列间的序列相同性百分数表示进行比对时，所比较的两条序列中相同氨基酸的百分数。利用本领域所知软件程序，例如参考文献84的7.7.18部分所描述的软件程序，可进行比对并确定同源性百分数或序列相同性百分数。优选通过史密斯-沃特曼(Smith-Waterman)同源性搜索算法进行比对，该算法使用仿射缺口搜索(affine gapsearch)，其中缺口开放罚12分，缺口延伸罚2分，BLOSUM矩阵计62分。参考文献85中公开了史密斯-沃特曼同源性搜索算法。
上文使用“GI”编号。GI号或者“基因信息识别号”(GenInfo Identifier)是NCBI将序列加入其数据库时，连续对每一序列记录指定的一串数字。GI编号与序列记录登录号没有相似之处。序列更新(如纠正或加入更多注释或信息)后，将接受新GI编号。因此与给定GI编号相关的序列是不变的。
当本发明涉及“表位”时，该表位可以是B细胞表位和/或T细胞表位。可凭经验鉴定此类表位(如使用PEPSCAN[86，87]或相似方法)，或可对其进行预测(如使用詹姆森-沃尔夫抗原性指数(Jameson-Wolf antigenic index)[88]、基于矩阵的方法[89]、MAPITOPE[90]、TEPITOPE[91，92]、神经网络[93]、OptiMer和EpiMer[94，95]、ADEPT[96]、T位点[97]、亲水性[98]、抗原性指数[99]或参考文献100-101中公开的方法等)。表位是抗体或T细胞受体的抗原结合位点识别并结合的抗原的某部分，它们也称作“抗原决定簇”。
附图说明
图1显示了从培养基中纯化的本发明突泡的电子显微镜照片。
图2显示了来自宋内志贺氏菌ΔtolRΔgalU的突泡的2D SDS-PAGE。
图3显示了不同所示菌株突泡的LPS(用抗核心抗体染色)。
图4显示了用被免疫小鼠的血清2D分离的突泡蛋白质的免疫斑点印迹。
图5显示了不同条件下生长的细菌的SDS-PAGE。
图6显示了突泡中的吸附蛋白。从左到右的泳道为：1)蛋白质分子量标记物，2)SsOMB 10μg，3)Ss OMB 2μg，4)吸附在明矾上1个月的Ss OMB 10μg。
图7显示了所示菌株的FACS数据。
具体实施方式
宋内志贺氏菌突变体的制备
采用λRed系统，使得野生型宋内志贺氏菌53G的tolR基因缺失[11，102]。制备用λRed质粒转化的感受态细胞，然后通过同源重组，用设计以抗生素抗性基因替换tolR基因的线性片段转化。通过对抗生素的抗性选择已整合该片段的克隆，通过PCR或其它技术验证tolR的缺失。然后可通过在37℃培养新克隆，去除温度敏感性λRed质粒。
已确证了在该ΔtolR突变体中TolR表达的缺少，与原先的野生型分离物相比，已验证该突变体在生长过程中将更多的突泡释放到培养基中。
用类似的途径使得galU基因缺失，以提供ΔgalU单突变体和AtolRΔgalU双突变体。经验证突变体释放的突泡具有缺少O抗原的缺陷型LPS。
用相同的方法制备带有修饰LPS的ΔtolRΔmsbB双突变株。
还从ΔtolR和ΔtolRΔmsbB菌株中去除了毒性质粒。
福氏志贺氏菌突变体的制备
如上针对宋内志贺氏菌所述，采用λRed系统使得福氏志贺氏菌的tolR基因缺失。福氏志贺氏菌中O抗原的生物合成通过包含部分rfbF和rfc以及完整rfbG基因的染色体片段的缺失(该缺失导致这三种基因全部失活)而被彻底破坏。采用λRed系统产生缺失，并缩写为ΔrfbG。
以相同的方式产生AtolRΔrfbG双突变体。
以相同的方式产生包含修饰的LPS的ΔtolRΔhtrB双突变体。
已从这些菌株中去除了毒性质粒。
突泡的纯化
在以下条件下进行双突变体ΔtolRΔgalU菌株的发酵：pH 7.1，37℃，通过受控的搅拌和设置最大充气量将溶氧保持在30％的饱和度。通过加入4M氢氧化铵控制pH。通过在发酵过程中加入10％PPG控制泡沫。培养基由以下组分组成：KH2PO4 5g/l，K2HPO4 20g/l和酵母提取物30g/l。采用高压灭菌对培养基进行灭菌后，加入甘油15g/l和MgSO4 2mM，然后进行接种。培养接种物为发酵罐体积的5％。发酵过程进行约13个小时，以600nm下的光学密度确定细胞浓度。
采用以下所定义的培养基进行宋内志贺氏菌ΔtolRΔmsbB双突变株的发酵过程：甘油30g/1，KH2PO4 13.3g/1，(NH4)2HPO4 4g/1，MgSO4·7H2O(1M)2ml，柠檬酸1.7g/1，CoCl2·6H2O2.5mg/1，MnCl2·4H2O15mg/1，CuCl2·2H2O1.5mg/1，H3BO3 3mg/1，Na2MoO4·2H2O2.5mg/1，Zn(CH3COO)2·2H2O13mg/1，柠檬酸铁2μM，硫胺素50mg/1，烟酸10mg/1，L-天冬氨酸2.5g/l。
采用两个连续TFF(正切流动过滤)步骤纯化在发酵液中产生的小囊泡：以0.22μm进行微过滤，然后以0.1μm进行第二次微过滤。在第一次过滤步骤中，通过经0.22μm孔径过滤盒的TFF从生物质中分离出小囊泡。先将该生物质浓缩4倍，用PBS进行5次透析过滤步骤后，在过滤液中收集小囊泡。在第二次过滤步骤中，使得从0.22μmTFF获得的过滤液进一步经0.1μm截留过滤盒进行微过滤，以从溶解蛋白质中纯化小囊泡。小囊泡不能通过过滤盒。经过5次透析过滤步骤后，收集含有小囊泡的保留物。
用TEM观察最终纯化产物(图1)。突泡具有约50nm直径的均一尺寸。
在与培养宋内志贺氏菌ΔtolRΔgalU相同的酵母提取物培养基中培养各种菌株，然后以相同的方式纯化来自福氏志贺氏菌突变体的突泡。
突泡的表征
通过蛋白质组方法证实了突泡为基本上纯的外膜。与通过破坏外膜获得的常规外膜囊泡(OMV)不同，所述突泡保留亲脂蛋白且基本上不含细胞质和内膜组分，.
用去污剂使得来自宋内志贺氏菌和福氏志贺氏菌菌株的突泡变性，并用LC-MS/MS方法鉴别蛋白质。或者，用SDS PAGE电泳或2D凝胶电泳分离突泡(图2)。从凝胶上切取可见条带和斑点，通过蛋白质质量指纹分析鉴定蛋白质。用密度计分析SDA-PAGE条带或来自2D凝胶的斑点研究不同蛋白质的相对量。
图3显示了ΔgalU突变株与野生型志贺氏菌和大肠杆菌(所有菌株均为ΔtolR背景)相比的LPS迁移率的改变。
采用基于表面消化的第二蛋白质组方法来表征膜蛋白的暴露部分。鉴定了与经突泡免疫的小鼠的血清反应的一系列蛋白质，已发现其中有多种蛋白质在许多菌株中是保守的。对于大部分完整外膜蛋白所知甚少。如下研究突泡的表在蛋白质组(surfome)：用蛋白酶处理，回收，然后通过LC-MS/MS鉴定释放的肽。由于突泡应代表了活的整菌细胞的表面，该图谱可代表宋内志贺氏菌表面上暴露的蛋白质。
通过这些方法和其它方法，在突泡中发现了表1中所列的129种蛋白质。
突泡的免疫原性
如图4所示，用来自ΔtolRΔgalU菌株的突泡免疫的小鼠产生的血清与该突泡的2D凝胶发生反应。因此，该突泡具有免疫原性。
小鼠接受2μg或10μg宋内志贺氏菌ΔtolRΔgalU突泡(以总蛋白质为基准测定)，加或不加佐剂(氢氧化铝或完全弗氏佐剂)。进行经典的ELISA方法以分析获自免疫研究的血清中的IgG的生产。所有小鼠组的血清均显示突泡特异性IgG的高水平生产。单独使用突泡或与佐剂联用时，未检测到IgG生产中的显著差异。用2μg的低剂量免疫的组与用10μg免疫的组显示出相同的突泡特异性IgG水平，这表明可采用低剂量疫苗，即每美元更多剂量。来自其它宋内志贺氏菌和福氏志贺氏菌菌株的突泡也类似地具有免疫原性。
测定抗突泡产生的血清与三种不同菌的反应性：宋内志贺氏菌G53、福氏志贺氏菌2a 2457T或福氏志贺氏菌5M90T。然后用标记的二抗对样品进行染色，并通过流式细胞法进行分析。如图7中所示，宋内志贺氏菌和福氏志贺氏菌菌株与血清发生交叉反应。
因此，该突泡方法在产生有效的低成本疫苗中具有很大的潜力，且可扩展到不同志贺氏菌菌株以产生广谱疫苗。
突泡吸附
将突泡与氢氧化铝(2mg/ml)组合进行吸附。将吸附材料储存在4℃下1天、1周或1个月。1天后突泡完全吸附，且甚至在1个月后都未显现出解吸附迹象(图6)。
限铁生长
图5显示在不同条件下生长的志贺氏菌表达的蛋白质。在第4泳道中，细菌在200μMFeSO4的存在下生长，在第5泳道中，培养物包含200μM联吡啶(dypiridyl)。插入的小图显示在限铁条件下三种蛋白质上调。这三种蛋白质鉴定为FepA外膜受体(GI 74311118)、大肠杆菌素I受体(GI 74312677)和推定铁嗜铁素受体(GI 74313972)。这些蛋白质在志贺氏菌属和肠杆菌科中非常保守，并具有潜在的高免疫原性。因此，在限铁条件下生长的志贺氏菌能导致突泡的释放，该突泡与正常生长条件下产生的突泡相比富含这些蛋白质。
应理解，仅以举例的方式描述了本发明，可对之进行修改而仍在本发明的范围和构思内。
表1
SEQ ID NO：70、71、73、74、76、111、112、114-129和131-135鉴别自宋内志贺氏菌ΔtolR突泡。SEQ ID NO：8-15和17-58鉴别自宋内志贺氏菌ΔtolRΔgalU突泡。SEQ ID NO：83、94、97和107鉴别自福氏志贺氏菌ΔtolR突泡。SEQ ID NO：68、69、72、75、77-82、84-93、95、96、98-106、108-10、113、130和136鉴别自福氏志贺氏菌ΔtolRΔrfbG突泡。SEQ ID NO：60-67鉴别自宋内志贺氏菌的表面消化物。
亚组1：SEQ ID NO：68、69、72、75、77-110、113、130和136。
亚组2：SEQ ID NO：8-15、17-58、60-67、70、71、73、74、76、11 1、1 12、114-129和131-135。
亚组3：SEQ ID NO：1-60。
NB：SEQ ID NO：18与SEQ ID NO：5相同；SEQ ID NO：33与SEQ ID NO：2相同；SEQ IDNO：9和16相关(约97％相同性)；SEQ ID NO：23和59相关(约98％相同性)。
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Gly Leu Gly Ala Asp Tyr Thr Tyr Ser Asn Gly Tyr Arg Asp Ala Asn
65 70 75 80
Gly Ile Asn Ser Asn Ala Thr Ser Ala Ser Leu Gln Leu Thr Gln Ser
85 90 95
Ile Phe Asp Met Ser Lys Trp Arg Ala Leu Thr Leu Gln Glu Lys Ala
100 105 110
Ala Gly Ile Gln Asp Val Thr Tyr Gln Thr Asp Gln Gln Thr Leu Ile
115 120 125
Leu Asn Thr Ala Thr Ala Tyr Phe Asn Val Leu Asn Ala Ile Asp Val
130 135 140
Leu Ser Tyr Thr Gln Ala Gln Lys Glu Ala Ile Tyr Arg Gln Leu Asp
145 150 155 160
Gln Thr Thr Gln Arg Phe Asn Val Gly Leu Val Ala Ile Thr Asp Val
165 170 175
Gln Asn Ala Arg Ala Gln Tyr Asp Thr Val Leu Ala Asn Glu Val Thr
180 185 190
Ala Arg Asn Asn Leu Asp Asn Ala Val Glu Gln Leu Arg Gln Ile Thr
195 200 205
Gly Asn Tyr Tyr Pro Glu Leu Ala Ala Leu Asn Val Glu Asn Phe Lys
210 215 220
Thr Asp Lys Pro Gln Pro Val Asn Ala Leu Leu Lys Glu Ala Glu Lys
225 230 235 240
Arg Asn Leu Ser Leu Leu Gln Ala Arg Leu Ser Gln Asp Leu Glu Arg
245 250 255
Glu Gln Ile Arg Gln Ala Gln Asp Gly His Leu Pro Thr Leu Asp Leu
260 265 270
Thr Ala Ser Thr Gly Ile Ser Asp Thr Ser Tyr Ser Gly Ser Lys Thr
275 280 285
Arg Gly Ala Ala Gly Thr Gln Tyr Asp Asp Ser Asn Met Gly Gln Asn
290 295 300
Lys Val Gly Leu Ser Phe Ser Leu Pro Ile Tyr Gln Gly Gly Met Val
305 310 315 320
Asn Ser Gln Val Lys Gln Ala Gln Tyr Asn Phe Val Gly Ala Ser Glu
325 330 335
Gln Leu Glu Ser Ala His Arg Ser Val Val Gln Thr Val Arg Ser Ser
340 345 350
Phe Asn Asn Ile Asn Ala Ser Ile Ser Ser Ile Asn Ala Tyr Lys Gln
355 360 365
Ala Val Val Ser Ala Gln Ser Ser Leu Asp Ala Met Glu Ala Gly Tyr
370 375 380
Ser Val Gly Thr Arg Thr Ile Val Asp Val Leu Asp Ala Thr Thr Thr
385 390 395 400
Leu Tyr Asn Ala Lys Gln Glu Leu Ala Asn Ala Arg Tyr Asn Tyr Leu
405 410 415
Ile Asn Gln Leu Asn Ile Lys Ser Ala Leu Gly Thr Leu Asn Glu Gln
420 425 430
Asp Leu Leu Ala Leu Asn Asn Ala Leu Ser Lys Pro Val Ser Thr Asn
435 440 445
Pro Glu Asn Val Ala Pro Gln Thr Pro Glu Gln Asn Ala Ile Ala Asp
450 455 460
Gly Tyr Ala Pro Asp Ser Pro Ala Pro Val Val Gln Gln Thr Ser Ala
465 470 475 480
Arg Thr Thr Thr Ser Asn Gly His Asn Pro Phe Arg Asn
485 490
<210> 9
<211> 368
<212> PRT
<213> 宋内志贺氏菌(Shigella sonnei)
<400> 9
Met Lys Val Lys Val Leu Ser Leu Leu Val Pro Ala Leu Leu Val Ala
1 5 10 15
Gly Ala Ala Asn Ala Ala Glu Val Tyr Asn Lys Asp Gly Asn Lys Leu
20 25 30
Asp Leu Tyr Gly Lys Val Asp Gly Leu His Tyr Phe Ser Asp Asn Lys
35 40 45
Ser Glu Asp Gly Asp Gln Thr Tyr Val Arg Leu Gly Phe Lys Gly Val
50 55 60
Thr Gln Val Thr Asp Gln Leu Thr Gly Tyr Gly Gln Trp Glu Tyr Gln
65 70 75 80
Ile Gln Gly Asn Thr Ser Glu Asp Asn Lys Glu Asn Ser Trp Thr Arg
85 90 95
Val Ala Phe Ala Gly Leu Lys Phe Gln Asp Val Gly Ser Phe Asp Tyr
100 105 110
Gly Arg Asn Tyr Gly Val Val Tyr Asp Val Thr Ser Trp Thr Asp Val
115 120 125
Leu Pro Glu Phe Gly Gly Asp Thr Tyr Gly Ser Asp Asn Phe Met Gln
130 135 140
Gln Arg Gly Asn Gly Phe Ala Thr Tyr Arg Asn Thr Asp Phe Phe Gly
145 150 155 160
Leu Val Asp Gly Leu Asn Phe Ala Val Gln Tyr Gln Gly Lys Asn Gly
165 170 175
Ser Val Ser Gly Glu Gly Met Thr Asn Asn Gly Arg Gly Ala Leu Arg
180 185 190
Gln Asn Gly Asp Gly Val Gly Gly Ser Ile Thr Tyr Asp Tyr Glu Gly
195 200 205
Phe Gly Ile Gly Gly Ala Ile Ser Ser Ser Lys Arg Thr Asp Asp Gln
210 215 220
Asn Ser Pro Leu Tyr Ile Gly Asn Gly Asp Arg Ala Glu Thr Tyr Thr
225 230 235 240
Gly Gly Leu Lys Tyr Asp Ala Asn Asn Ile Tyr Leu Ala Ala Gln Tyr
245 250 255
Thr Gln Thr Tyr Asn Ala Thr Arg Val Gly Ser Leu Gly Trp Ala Asn
260 265 270
Lys Ala Gln Asn Phe Glu Ala Val Ala Gln Tyr Gln Phe Asp Phe Gly
275 280 285
Leu Arg Pro Ser Val Ala Tyr Leu Gln Ser Lys Gly Lys Asn Leu Gly
290 295 300
Thr Ile Ala Gly Arg Asn Tyr Asp Asp Glu Asp Ile Leu Lys Tyr Val
305 310 315 320
Asp Val Gly Ala Thr Tyr Tyr Phe Asn Lys Asn Met Ser Thr Tyr Val
325 330 335
Asp Tyr Lys Ile Asn Leu Leu Asp Asp Asn Gln Phe Thr Arg Asp Ala
340 345 350
Gly Ile Asn Thr Asp Asn Ile Val Ala Leu Gly Leu Val Tyr Gln Phe
355 360 365
<210> 10
<211> 346
<212> PRT
<213> 宋内志贺氏菌
<400> 10
Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala
1 5 10 15
Thr Val Ala Gln Ala Ala Pro Lys Asp Asn Thr Trp Tyr Thr Gly Ala
20 25 30
Lys Leu Gly Trp Ser Gln Tyr His Asp Thr Gly Phe Ile Asn Asn Asn
35 40 45
Gly Pro Thr His Glu Asn Gln Leu Gly Ala Gly Ala Phe Gly Gly Tyr
50 55 60
Gln Val Asn Pro Tyr Val Gly Phe Glu Met Gly Tyr Asp Trp Leu Gly
65 70 75 80
Arg Met Pro Tyr Lys Gly Ser Val Glu Asn Gly Ala Tyr Lys Ala Gln
85 90 95
Gly Val Gln Leu Thr Ala Lys Leu Gly Tyr Pro Ile Thr Asp Asp Leu
100 105 110
Asp Ile Tyr Thr Arg Leu Gly Gly Met Val Trp Arg Ala Asp Thr Lys
115 120 125
Ser Asn Val Tyr Gly Lys Asn His Asp Thr Gly Val Ser Pro Val Phe
130 135 140
Ala Gly Gly Val Glu Tyr Ala Ile Thr Pro Glu Ile Ala Thr Arg Leu
145 150 155 160
Glu Tyr Gln Trp Thr Asn Asn Ile Gly Asp Ala His Thr Ile Gly Thr
165 170 175
Arg Pro Asp Asn Gly Met Leu Ser Leu Gly Val Ser Tyr Arg Phe Gly
180 185 190
Gln Gly Glu Ala Ala Pro Val Val Ala Pro Ala Pro Ala Pro Ala Pro
195 200 205
Glu Val Gln Thr Lys His Phe Thr Leu Lys Ser Asp Val Leu Phe Asn
210 215 220
Phe Asn Lys Ala Thr Leu Lys Pro Glu Gly Gln Ala Ala Leu Asp Gln
225 230 235 240
Leu Tyr Ser Gln Leu Ser Asn Leu Asp Pro Lys Asp Gly Ser Val Val
245 250 255
Val Leu Gly Tyr Thr Asp Arg Ile Gly Ser Asp Ala Tyr Asn Gln Gly
260 265 270
Leu Ser Glu Arg Arg Ala Gln Ser Val Val Asp Tyr Leu Ile Ser Lys
275 280 285
Gly Ile Pro Ala Asp Lys Ile Ser Ala Arg Gly Met Gly Glu Ser Asn
290 295 300
Pro Val Thr Gly Asn Thr Cys Asp Asn Val Lys Gln Arg Ala Ala Leu
305 310 315 320
Ile Asp Cys Leu Ala Pro Asp Arg Arg Val Glu Ile Glu Val Lys Gly
325 330 335
Ile Lys Asp Val Val Thr Gln Pro Gln Ala
340 345
<210> 11
<211> 407
<212> PRT
<213> 福氏志贺氏菌
<400> 11
Met Ser Ile Asp Phe Thr Pro Gly Ile Ile Asn Thr Tyr His Gly Asp
1 5 10 15
Ile Tyr Asn Cys Thr Thr Asn Thr Asp Asn Val Lys Thr Pro Asp Thr
20 25 30
Pro Lys Trp Pro Cys Asp Asn Trp Glu Glu Gln Gln Pro Ile Asn Ser
35 40 45
Thr Phe Ser Gly Glu Gly Tyr Asn Pro Glu Gln Phe Asp Leu Ala Gln
50 55 60
Gln Gln Leu Gln Gln Ile Asn Ala Cys His Thr Asn Thr Thr Tyr Thr
65 70 75 80
Asn Ala Asp Tyr Ser Lys Val Val Val Gln Leu Val Ser Leu Ile Asn
85 90 95
Asn Ile Glu Thr Ile Ser Ser Thr Pro Leu Thr Gln Gln Thr Gln Ser
100 105 110
Ile Leu Asn Gln Ile His Asn Ile Arg Tyr Glu Lys Asp Lys Asp Ser
115 120 125
Val Cys Gln Ile Ile Val Val Val Asn Pro Glu Pro Asn Asn Pro Ile
130 135 140
Ile Thr Lys Ile Leu Val Glu Glu Gly Ile Pro Glu Arg Phe Ser Val
145 150 155 160
Gln Thr Val Ser Ser Asp Asn Lys Asn Phe Ala Gly Gln Arg Ala Asp
165 170 175
Leu Pro Thr Asp Ile Arg Asp Ile Gln Ser Leu Tyr Leu Lys Met Ala
180 185 190
Lys Leu Tyr Ile Glu His Ser Lys Asn Glu Asn Arg Met Glu Ala Leu
195 200 205
Ala Gly Cys Asp Phe Ile Asp Phe Asn Met Thr Gly Gln Asp Met Ser
210 215 220
Lys Leu Val Leu Thr Leu Ser Lys Phe Tyr Phe Glu Asp Leu Leu Asn
225 230 235 240
Ile Asn Phe Thr Asp Ala Asn Leu Phe Asn Thr Ile Phe Ser His Lys
245 250 255
Glu Asn Pro Ile Pro Lys Leu His Lys Tyr Glu Gln His Leu Asp Lys
260 265 270
Gln Ile Asn Gly Leu Phe Ser Thr Leu Leu Thr Ile Asn Asp Asn Ser
275 280 285
Leu Arg Ala Lys Ala Glu Ile Ala Ser Arg Ile Ile Asp Phe Leu Glu
290 295 300
Ala Lys Val Val Asn Leu Ser Phe Asp Asp Ile Leu Lys Tyr Lys Gln
305 310 315 320
Glu Phe Lys Lys Ile Cys Tyr Lys Gln Leu Gln Glu Phe Thr Thr Pro
325 330 335
Ser Leu Tyr Asn Lys Ile Gln Lys Trp Ala Thr Met Ser Lys Asn Glu
340 345 350
Phe Ile Ala Phe His Tyr Glu Thr Leu Gln Pro Glu Lys Ile Ser Tyr
355 360 365
Pro Phe Tyr Leu Lys Arg Asp Leu Pro Asn Glu Lys Asp Ile Asn Tyr
370 375 380
Gly Val Glu Ile Glu Ile Pro Ser Gly Lys Arg Ile Arg Leu Ser Asn
385 390 395 400
His Tyr Gln Asn Ile Ile Pro
405
<210> 12
<211> 171
<212> PRT
<213> 福氏志贺氏菌
<400> 12
Met Lys Lys Ile Ala Cys Leu Ser Ala Leu Ala Ala Val Leu Ala Phe
1 5 10 15
Thr Ala Gly Thr Ser Val Ala Ala Thr Ser Thr Val Thr Gly Gly Tyr
20 25 30
Ala Gln Ser Asp Ala Gln Gly Gln Met Asn Lys Met Gly Gly Phe Asn
35 40 45
Leu Lys Tyr Arg Tyr Glu Glu Asp Asn Ser Pro Leu Gly Val Ile Gly
50 55 60
Ser Phe Thr Tyr Thr Glu Lys Ser Arg Thr Ala Ser Ser Gly Asp Tyr
65 70 75 80
Asn Lys Asn Gln Tyr Tyr Gly Ile Thr Ala Gly Pro Ala Tyr Arg Ile
85 90 95
Asn Asp Trp Ala Ser Ile Tyr Gly Val Val Gly Val Gly Tyr Gly Lys
100 105 110
Phe Gln Thr Thr Glu Tyr Pro Thr Tyr Lys His Asp Thr Ser Asp Tyr
115 120 125
Gly Phe Ser Tyr Gly Ala Gly Leu Gln Phe Asn Pro Met Glu Asn Val
130 135 140
Ala Leu Asp Phe Ser Tyr Glu Gln Ser Arg Ile Arg Ser Val Asp Val
145 150 155 160
Gly Thr Trp Ile Ala Gly Val Gly Tyr Arg Phe
165 170
<210> 13
<211> 155
<212> PRT
<213> 福氏志贺氏菌
<400> 13
Met Ile Lys Arg Val Leu Val Val Ser Met Val Gly Leu Ser Leu Val
1 5 10 15
Gly Cys Val Asn Asn Asp Thr Leu Ser Gly Asp Val Tyr Thr Ala Ser
20 25 30
Glu Ala Lys Gln Val Gln Asn Val Ser Tyr Gly Thr Ile Val Asn Val
35 40 45
Arg Pro Val Gln Ile Gln Gly Gly Asp Asp Ser Asn Val Ile Gly Ala
50 55 60
Ile Gly Gly Ala Val Leu Gly Gly Phe Leu Gly Asn Thr Val Gly Gly
65 70 75 80
Gly Thr Gly Arg Ser Leu Ala Thr Ala Ala Gly Ala Val Ala Gly Gly
85 90 95
Val Ala Gly Gln Gly Val Gln Ser Ala Met Asn Lys Thr Gln Gly Val
100 105 110
Glu Leu Glu Ile Arg Lys Asp Asp Gly Asn Thr Ile Met Val Val Gln
115 120 125
Lys Gln Gly Asn Thr Arg Phe Ser Pro Gly Gln Arg Val Val Leu Ala
130 135 140
Ser Asn Gly Ser Gln Val Thr Val Ser Pro Arg
145 150 155
<210> 14
<211> 207
<212> PRT
<213> 福氏志贺氏菌
<400> 14
Met Pro Leu Pro Asp Phe Arg Leu Ile Arg Leu Leu Pro Leu Ala Ala
1 5 10 15
Leu Val Leu Thr Ala Cys Ser Val Thr Thr Pro Lys Gly Pro Gly Lys
20 25 30
Ser Pro Asp Ser Pro Gln Trp Arg Gln His Gln Gln Asp Val Arg Asn
35 40 45
Leu Asn Gln Tyr Gln Thr Arg Gly Ala Phe Ala Tyr Ile Ser Asp Gln
50 55 60
Gln Lys Val Tyr Ala Arg Phe Phe Trp Gln Gln Thr Gly Gln Asp Arg
65 70 75 80
Tyr Arg Leu Leu Leu Thr Asn Pro Leu Gly Ser Thr Glu Leu Glu Leu
85 90 95
Asn Ala Gln Pro Gly Asn Val Gln Leu Val Asp Asn Lys Gly Gln Arg
100 105 110
Tyr Thr Ala Asp Asp Ala Glu Glu Met Ile Gly Lys Leu Thr Gly Met
115 120 125
Pro Ile Pro Leu Asn Ser Leu Arg Gln Trp Ile Leu Gly Leu Pro Gly
130 135 140
Asp Ala Thr Asp Tyr Lys Leu Asp Asp Gln Tyr Arg Leu Ser Glu Ile
145 150 155 160
Thr Tyr Ser Gln Asn Gly Lys Asn Trp Lys Val Val Tyr Gly Gly Tyr
165 170 175
Asp Thr Lys Thr Gln Pro Ala Met Pro Ala Asn Met Glu Leu Thr Asp
180 185 190
Gly Gly Gln Arg Ile Lys Leu Lys Met Asp Asn Trp Ile Val Lys
195 200 205
<210> 15
<211> 810
<212> PRT
<213> 福氏志贺氏菌
<400> 15
Met Ala Met Lys Lys Leu Leu Ile Ala Ser Leu Leu Phe Ser Ser Ala
1 5 10 15
Thr Val Tyr Gly Ala Glu Gly Phe Val Val Lys Asp Ile His Phe Glu
20 25 30
Gly Leu Gln Arg Val Ala Val Gly Ala Ala Leu Leu Ser Met Pro Val
35 40 45
Arg Thr Gly Asp Thr Val Asn Asp Glu Asp Ile Ser Asn Thr Ile Arg
50 55 60
Ala Leu Phe Ala Thr Gly Asn Phe Glu Asp Val Arg Val Leu Arg Asp
65 70 75 80
Gly Asp Thr Leu Leu Val Gln Val Lys Glu Arg Pro Thr Ile Ala Ser
85 90 95
Ile Thr Phe Ser Gly Asn Lys Ser Val Lys Asp Asp Met Leu Lys Gln
100 105 110
Asn Leu Glu Ala Ser Gly Val Arg Val Gly Glu Ser Leu Asp Arg Thr
115 120 125
Thr Ile Ala Asp Ile Glu Lys Gly Leu Glu Asp Phe Tyr Tyr Ser Val
130 135 140
Gly Lys Tyr Ser Ala Ser Val Lys Ala Val Val Thr Pro Leu Pro Arg
145 150 155 160
Asn Arg Val Asp Leu Lys Leu Val Phe Gln Glu Gly Val Ser Ala Glu
165 170 175
Ile Gln Gln Ile Asn Ile Val Gly Asn His Ala Phe Thr Thr Asp Glu
180 185 190
Leu Ile Ser His Phe Gln Leu Arg Asp Glu Val Pro Trp Trp Asn Val
195 200 205
Val Gly Asp Arg Lys Tyr Gln Lys Gln Lys Leu Ala Gly Asp Leu Glu
210 215 220
Thr Leu Arg Ser Tyr Tyr Leu Asp Arg Gly Tyr Ala Arg Phe Asn Ile
225 230 235 240
Asp Ser Thr Gln Val Ser Leu Thr Pro Asp Lys Lys Gly Ile Tyr Val
245 250 255
Thr Val Asn Ile Thr Glu Gly Asp Gln Tyr Lys Leu Ser Gly Val Glu
260 265 270
Val Ser Gly Asn Leu Ala Gly His Ser Ala Glu Ile Glu Gln Leu Thr
275 280 285
Lys Ile Glu Pro Gly Glu Leu Tyr Asn Gly Thr Lys Val Thr Lys Met
290 295 300
Glu Asp Asp Ile Lys Lys Leu Leu Gly Arg Tyr Gly Tyr Ala Tyr Pro
305 310 315 320
Arg Val Gln Ser Met Pro Glu Ile Asn Asp Ala Asp Lys Thr Val Lys
325 330 335
Leu Arg Val Asn Val Asp Ala Gly Asn Arg Phe Tyr Val Arg Lys Ile
340 345 350
Arg Phe Glu Gly Asn Asp Thr Ser Lys Asp Ala Val Leu Arg Arg Glu
355 360 365
Met Arg Gln Met Glu Gly Ala Trp Leu Gly Ser Asp Leu Val Asp Gln
370 375 380
Gly Lys Glu Arg Leu Asn Arg Leu Gly Phe Phe Glu Thr Val Asp Thr
385 390 395 400
Asp Thr Gln Arg Val Pro Gly Ser Pro Asp Gln Val Asp Val Val Tyr
405 410 415
Lys Val Lys Glu Arg Asn Thr Gly Ser Phe Asn Phe Gly Ile Gly Tyr
420 425 430
Gly Thr Glu Ser Gly Val Ser Phe Gln Ala Gly Val Gln Gln Asp Asn
435 440 445
Trp Leu Gly Thr Gly Tyr Ala Val Gly Ile Asn Gly Thr Lys Asn Asp
450 455 460
Tyr Gln Thr Tyr Ala Glu Leu Ser Val Thr Asn Pro Tyr Phe Thr Val
465 470 475 480
Asp Gly Val Ser Leu Gly Gly Arg Leu Phe Tyr Asn Asp Phe Gln Ala
485 490 495
Asp Asp Ala Asp Leu Ser Asp Tyr Thr Asn Lys Ser Tyr Gly Thr Asp
500 505 510
Val Thr Leu Gly Phe Pro Ile Asn Glu Tyr Asn Ser Leu Arg Ala Gly
515 520 525
Leu Gly Tyr Val His Asn Ser Leu Ser Asn Met Gln Pro Gln Val Ala
530 535 540
Met Trp Arg Tyr Leu Tyr Ser Met Gly Glu His Pro Ser Thr Ser Asp
545 550 555 560
Gln Asp Asn Ser Phe Lys Thr Asp Asp Phe Thr Phe Asn Tyr Gly Trp
565 570 575
Thr Tyr Asn Lys Leu Asp Arg Gly Tyr Phe Pro Thr Asp Gly Ser Arg
580 585 590
Val Asn Leu Thr Gly Lys Val Thr Ile Pro Gly Ser Asp Asn Glu Tyr
595 600 605
Tyr Lys Val Thr Leu Asp Thr Ala Thr Tyr Val Pro Ile Asp Asp Asp
610 615 620
His Lys Trp Val Val Leu Gly Arg Thr Arg Trp Gly Tyr Gly Asp Gly
625 630 635 640
Leu Gly Gly Lys Glu Met Pro Phe Tyr Glu Asn Phe Tyr Ala Gly Gly
645 650 655
Ser Ser Thr Val Arg Gly Phe Gln Ser Asn Thr Ile Gly Pro Lys Ala
660 665 670
Val Tyr Phe Pro His Gln Ala Ser Asn Tyr Asp Pro Asp Tyr Asp Tyr
675 680 685
Glu Cys Ala Thr Gln Asp Gly Ala Lys Asp Leu Cys Lys Ser Asp Asp
690 695 700
Ala Val Gly Gly Asn Ala Met Ala Val Ala Ser Leu Glu Phe Ile Thr
705 710 715 720
Pro Thr Pro Phe Ile Ser Asp Lys Tyr Ala Asn Ser Val Arg Thr Ser
725 730 735
Phe Phe Trp Asp Met Gly Thr Val Trp Asp Thr Asn Trp Asp Ser Ser
740 745 750
Gln Tyr Ser Gly Tyr Pro Asp Tyr Ser Asp Pro Ser Asn Ile Arg Met
755 760 765
Ser Ala Gly Ile Ala Leu Gln Trp Met Ser Pro Leu Gly Pro Leu Val
770 775 780
Phe Ser Tyr Ala Gln Pro Phe Lys Lys Tyr Asp Gly Asp Lys Ala Glu
785 790 795 800
Gln Phe Gln Phe Asn Ile Gly Lys Thr Trp
805 810
<210> 16
<211> 376
<212> PRT
<213> 鲍氏志贺氏菌(Shigella boydii)
<400> 16
Met Lys Val Lys Val Leu Ser Leu Leu Val Pro Ala Leu Leu Val Ala
1 5 10 15
Gly Ala Ala Asn Ala Ala Glu Val Tyr Asn Lys Asp Gly Asn Lys Leu
20 25 30
Asp Leu Tyr Gly Lys Val Asp Gly Leu His Tyr Phe Ser Asp Asn Lys
35 40 45
Ser Glu Asp Gly Asp Gln Thr Tyr Val Arg Leu Gly Phe Lys Gly Glu
50 55 60
Thr Gln Val Thr Asp Gln Leu Thr Gly Tyr Gly Gln Trp Glu Tyr Gln
65 70 75 80
Ile Gln Gly Asn Thr Ser Glu Asp Asn Lys Glu Asn Ser Trp Thr Arg
85 90 95
Val Ala Phe Ala Gly Leu Lys Phe Gln Asp Val Gly Ser Phe Asp Tyr
100 105 110
Gly Arg Asn Tyr Gly Val Val Tyr Asp Val Thr Ser Trp Thr Asp Val
115 120 125
Leu Pro Glu Phe Gly Gly Asp Thr Tyr Gly Ser Asp Asn Phe Met Gln
130 135 140
Gln Arg Gly Asn Gly Phe Ala Thr Tyr Arg Asn Thr Asp Phe Phe Gly
145 150 155 160
Leu Val Asp Gly Leu Asn Phe Ala Val Gln Tyr Gln Gly Lys Asn Gly
165 170 175
Ser Val Ser Gly Glu Gly Met Thr Asn Asn Gly Arg Gly Ala Leu Arg
180 185 190
Gln Asn Gly Asp Gly Val Gly Gly Ser Ile Thr Tyr Asp Tyr Glu Gly
195 200 205
Phe Gly Ile Gly Ala Ala Val Ser Ser Ser Lys Arg Thr Asp Asp Gln
210 215 220
Asn Gly Ser Tyr Ile Ser Asn Gly Val Val Arg Asn Tyr Ile Gly Thr
225 230 235 240
Gly Asp Arg Ala Glu Thr Tyr Thr Gly Gly Leu Lys Tyr Asp Ala Asn
245 250 255
Asn Ile Tyr Leu Ala Ala Gln Tyr Thr Gln Thr Tyr Asn Ala Thr Arg
260 265 270
Val Gly Ser Leu Gly Trp Ala Asn Lys Ala Gln Asn Phe Glu Ala Val
275 280 285
Ala Gln Tyr Gln Phe Asp Phe Gly Leu Arg Pro Ser Val Ala Tyr Leu
290 295 300
Gln Ser Lys Gly Lys Asn Leu Gly Val Ile Asn Gly Arg Asn Tyr Asp
305 310 315 320
Asp Glu Asp Ile Leu Lys Tyr Val Asp Val Gly Ala Thr Tyr Tyr Phe
325 330 335
Asn Lys Asn Met Ser Thr Tyr Val Asp Tyr Lys Ile Asn Leu Leu Asp
340 345 350
Asp Asn Gln Phe Thr Arg Asp Ala Gly Ile Asn Thr Asp Asn Ile Val
355 360 365
Ala Leu Gly Leu Val Tyr Gln Phe
370 375
<210> 17
<211> 78
<212> PRT
<213> 福氏志贺氏菌
<400> 17
Met Lys Ala Thr Lys Leu Val Leu Gly Ala Val Ile Leu Gly Ser Thr
1 5 10 15
Leu Leu Ala Gly Cys Ser Ser Asn Ala Lys Ile Asp Gln Leu Ser Ser
20 25 30
Asp Val Gln Thr Leu Asn Ala Lys Val Asp Gln Leu Ser Asn Asp Val
35 40 45
Asn Ala Met Arg Ser Asp Val Gln Ala Ala Lys Asp Asp Ala Ala Arg
50 55 60
Ala Asn Gln Arg Leu Asp Asn Met Ala Thr Lys Tyr Arg Lys
65 70 75
<210> 18
<211> 173
<212> PRT
<213> 福氏志贺氏菌
<400> 18
Met Gln Leu Asn Lys Val Leu Lys Gly Leu Met Ile Ala Leu Pro Val
1 5 10 15
Met Ala Ile Ala Ala Cys Ser Ser Asn Lys Asn Ala Ser Asn Asp Gly
20 25 30
Ser Glu Gly Met Leu Gly Ala Gly Thr Gly Met Asp Ala Asn Gly Gly
35 40 45
Asn Gly Asn Met Ser Ser Glu Glu Gln Ala Arg Leu Gln Met Gln Gln
50 55 60
Leu Gln Gln Asn Asn Ile Val Tyr Phe Asp Leu Asp Lys Tyr Asp Ile
65 70 75 80
Arg Ser Asp Phe Ala Gln Met Leu Asp Ala His Ala Asn Phe Leu Arg
85 90 95
Ser Asn Pro Ser Tyr Lys Val Thr Val Glu Gly His Ala Asp Glu Arg
100 105 110
Gly Thr Pro Glu Tyr Asn Ile Ser Leu Gly Glu Arg Arg Ala Asn Ala
115 120 125
Val Lys Met Tyr Leu Gln Gly Lys Gly Val Ser Ala Asp Gln Ile Ser
130 135 140
Ile Val Ser Tyr Gly Lys Glu Lys Pro Ala Val Leu Gly His Asp Glu
145 150 155 160
Ala Ala Tyr Ser Lys Asn Arg Arg Ala Val Leu Val Tyr
165 170
<210> 19
<211> 48
<212> PRT
<213> 福氏志贺氏菌
<400> 19
Met Val Lys Lys Thr Ile Ala Ala Ile Phe Ser Val Leu Val Leu Ser
1 5 10 15
Thr Val Leu Thr Ala Cys Asn Thr Thr Arg Gly Val Gly Glu Asp Ile
20 25 30
Ser Asp Gly Gly Asn Ala Ile Ser Gly Ala Ala Thr Lys Ala Gln Gln
35 40 45
<210> 20
<211> 137
<212> PRT
<213> 福氏志贺氏菌
<400> 20
Met Lys Lys Leu Ala Ile Ala Gly Ala Leu Met Leu Leu Ala Gly Cys
1 5 10 15
Ala Glu Val Glu Asn Tyr Asn Asn Val Val Lys Thr Pro Ala Pro Asp
20 25 30
Trp Leu Ala Gly Tyr Trp Gln Thr Lys Gly Pro Gln Arg Ala Leu Val
35 40 45
Ser Pro Glu Ala Ile Gly Ser Leu Ile Val Thr Lys Glu Gly Asp Thr
50 55 60
Leu Asp Cys Arg Gln Trp Gln Arg Gly Ile Ala Val Pro Gly Lys Leu
65 70 75 80
Thr Leu Met Ser Asp Asp Leu Thr Asn Val Thr Val Lys Arg Glu Leu
85 90 95
Tyr Glu Val Glu Arg Asp Gly Asn Thr Ile Glu Tyr Asp Gly Met Thr
100 105 110
Met Glu Arg Val Asp Arg Pro Thr Ala Glu Cys Ala Ala Ala Leu Asp
115 120 125
Lys Ala Pro Leu Pro Thr Pro Leu Pro
130 135
<210> 21
<211> 130
<212> PRT
<213> 福氏志贺氏菌
<400> 21
Met Lys Lys Phe Ala Ala Val Ile Ala Val Met Ala Leu Cys Ser Ala
1 5 10 15
Pro Val Met Ala Ala Glu Gln Gly Gly Phe Ser Gly Pro Ser Val Thr
20 25 30
Gln Ser Gln Ala Gly Gly Phe Gln Gly Pro Asn Gly Ser Val Thr Thr
35 40 45
Val Glu Ser Ala Lys Ser Leu Arg Asp Asp Thr Trp Val Thr Leu Arg
50 55 60
Gly Asn Ile Val Glu Arg Ile Ser Asp Asp Leu Tyr Val Phe Lys Asp
65 70 75 80
Ala Ser Gly Thr Ile Asn Val Asp Ile Asp His Lys Arg Trp Asn Gly
85 90 95
Val Thr Val Thr Pro Lys Asp Thr Val Glu Ile Gln Gly Glu Val Asp
100 105 110
Lys Asp Trp Asn Ser Val Glu Ile Asp Val Lys Gln Ile Arg Lys Val
115 120 125
Asn Pro
130
<210> 22
<211> 128
<212> PRT
<213> 福氏志贺氏菌
<400> 22
Met Ala Ser Ser Ser Leu Ile Met Gly Asn Asn Met His Val Lys Tyr
1 5 10 15
Leu Ala Gly Ile Val Gly Ala Ala Leu Leu Met Ala Gly Cys Ser Ser
20 25 30
Ser Asn Glu Leu Ser Ala Ala Gly Gln Ser Val Arg Ile Val Asp Glu
35 40 45
Gln Pro Gly Ala Glu Cys Gln Leu Ile Gly Thr Ala Thr Gly Lys Gln
50 55 60
Ser Asn Trp Leu Ser Gly Gln His Gly Glu Glu Gly Gly Ser Met Arg
65 70 75 80
Gly Ala Ala Asn Asp Leu Arg Asn Gln Ala Ala Ala Met Gly Gly Asn
85 90 95
Val Ile Tyr Gly Ile Ser Ser Pro Ser Gln Gly Met Leu Ser Ser Phe
100 105 110
Val Pro Thr Asp Ser Gln Ile Ile Gly Gln Val Tyr Lys Cys Pro Asn
115 120 125
<210> 23
<211> 190
<212> PRT
<213> 福氏志贺氏菌
<400> 23
Met Lys Leu Val His Met Ala Ser Gly Leu Ala Val Ala Ile Ala Leu
1 5 10 15
Ala Ala Cys Ala Asp Lys Ser Ala Asp Ile Gln Thr Pro Ala Pro Ala
20 25 30
Ala Asn Thr Ser Ile Ser Ala Thr Gln Gln Pro Ala Ile Gln Gln Pro
35 40 45
Asn Val Ser Gly Thr Val Trp Ile Arg Gln Lys Val Ala Leu Pro Pro
50 55 60
Asp Ala Val Leu Thr Val Thr Leu Ser Asp Ala Ser Leu Ala Asp Ala
65 70 75 80
Pro Ser Lys Val Leu Ala Gln Lys Ala Val Arg Thr Glu Gly Lys Gln
85 90 95
Ser Pro Phe Ser Phe Val Leu Pro Phe Asn Pro Ala Asp Val Gln Pro
100 105 110
Asn Ala Arg Ile Leu Leu Ser Ala Ala Ile Thr Val Asn Asp Lys Leu
115 120 125
Val Phe Ile Thr Asp Thr Val Gln Pro Val Ile Asn Gln Gly Gly Thr
130 135 140
Lys Ala Asp Leu Thr Leu Val Pro Val Gln Gln Thr Ala Val Pro Val
145 150 155 160
Gln Ala Ser Gly Gly Ala Thr Thr Thr Val Pro Ser Thr Ser Pro Thr
165 170 175
Gln Val Asn Pro Ser Ser Ala Val Pro Ala Pro Thr Gln Tyr
180 185 190
<210> 24
<211> 191
<212> PRT
<213> 福氏志贺氏菌
<400> 24
Met Lys Ser Leu Arg Leu Met Leu Cys Ala Met Pro Leu Met Leu Thr
1 5 10 15
Gly Cys Ser Thr Met Ser Ser Val Asn Trp Ser Ala Ala Asn Pro Trp
20 25 30
Asn Trp Phe Gly Ser Ser Thr Lys Val Ser Glu Gln Gly Val Gly Glu
35 40 45
Leu Thr Ala Ser Thr Pro Leu Gln Glu Gln Ala Ile Ala Asp Ala Leu
50 55 60
Asp Gly Asp Tyr Arg Leu Arg Ser Gly Met Lys Thr Thr Asn Gly Asn
65 70 75 80
Val Val Arg Phe Phe Glu Val Met Lys Gly Asp Asn Val Ala Met Val
85 90 95
Ile Asn Gly Asp Gln Gly Thr Ile Ser Arg Ile Asp Val Leu Asp Ser
100 105 110
Asp Ile Pro Ala Asp Thr Gly Val Lys Ile Gly Thr Pro Phe Ser Asp
115 120 125
Leu Tyr Ser Lys Ala Phe Gly Asn Cys Gln Lys Ala Asp Gly Asp Asp
130 135 140
Asn Arg Ala Val Glu Cys Lys Ala Glu Gly Ser Gln His Ile Ser Tyr
145 150 155 160
Gln Phe Ser Gly Glu Trp Arg Gly Pro Glu Gly Leu Met Pro Ser Asp
165 170 175
Asp Thr Leu Lys Asn Trp Lys Val Ser Lys Ile Ile Trp Arg Arg
180 185 190
<210> 25
<211> 76
<212> PRT
<213> 宋内志贺氏菌
<400> 25
Met Met Lys Lys Thr Ala Ala Ile Ile Ser Ala Cys Met Leu Thr Phe
1 5 10 15
Ala Leu Ser Ala Cys Ser Gly Ser Asn Tyr Val Met His Thr Asn Asp
20 25 30
Gly Arg Thr Ile Val Ser Asp Gly Lys Pro Gln Thr Asp Asn Asp Thr
35 40 45
Gly Met Ile Ser Tyr Lys Asp Ala Asn Gly Asn Lys Gln Gln Ile Asn
50 55 60
Arg Thr Asp Val Lys Glu Met Val Glu Leu Asp Gln
65 70 75
<210> 26
<211> 344
<212> PRT
<213> 福氏志贺氏菌
<400> 26
Met Ala Tyr Ser Val Gln Lys Ser Arg Leu Ala Lys Val Ala Gly Val
1 5 10 15
Ser Leu Val Leu Leu Leu Ala Ala Cys Ser Ser Asp Ser Arg Tyr Lys
20 25 30
Arg Gln Val Ser Gly Asp Glu Ala Tyr Leu Glu Ala Ala Pro Leu Ala
35 40 45
Glu Leu His Ala Pro Ala Gly Met Ile Leu Pro Val Thr Ser Gly Asp
50 55 60
Tyr Ala Ile Pro Val Thr Asn Gly Ser Gly Ala Val Gly Lys Ala Leu
65 70 75 80
Asp Ile Arg Pro Pro Ala Gln Pro Leu Ala Leu Val Ser Gly Ala Arg
85 90 95
Thr Gln Phe Thr Gly Asp Thr Ala Ser Leu Leu Val Glu Asn Gly Arg
100 105 110
Gly Asn Thr Leu Trp Pro Gln Val Val Ser Val Leu Gln Ala Lys Asn
115 120 125
Tyr Thr Ile Thr Gln Arg Asp Asp Ala Gly Gln Thr Leu Thr Thr Asp
130 135 140
Trp Val Gln Trp Asn Arg Leu Asp Glu Asp Glu Gln Tyr Arg Gly Arg
145 150 155 160
Tyr Gln Ile Ser Val Lys Pro Gln Gly Tyr Gln Gln Ala Val Thr Val
165 170 175
Lys Leu Leu Asn Leu Glu Gln Ala Gly Lys Pro Val Ala Asp Ala Ala
180 185 190
Ser Met Gln Arg Tyr Ser Thr Glu Met Met Asn Val Ile Ser Ala Gly
195 200 205
Leu Asp Lys Ser Ala Thr Asp Ala Ala Asn Ala Ala Gln Asn Arg Ala
210 215 220
Ser Thr Thr Met Asp Val Gln Ser Ala Ala Asp Asp Thr Gly Leu Pro
225 230 235 240
Met Leu Val Val Arg Gly Pro Phe Asn Val Val Trp Gln Arg Leu Pro
245 250 255
Ala Ala Leu Glu Lys Val Gly Met Lys Val Thr Asp Ser Thr Arg Ser
260 265 270
Gln Gly Asn Met Ala Val Thr Tyr Lys Pro Leu Ser Asp Ser Asp Trp
275 280 285
Gln Glu Leu Gly Ala Ser Asp Pro Gly Leu Ala Ser Gly Asp Tyr Lys
290 295 300
Leu Gln Val Gly Asp Leu Asp Asn Arg Ser Ser Leu Gln Phe Ile Asp
305 310 315 320
Pro Lys Gly His Thr Leu Thr Gln Ser Gln Asn Asp Ala Leu Val Ala
325 330 335
Val Phe Gln Ala Ala Phe Ser Lys
340
<210> 27
<211> 139
<212> PRT
<213> 宋内志贺氏菌
<400> 27
Met Arg Arg Thr Gly Asn Lys Leu Cys Leu Ile Ala Met Ile Thr Ala
1 5 10 15
Thr Val Ala Leu Thr Ala Cys Thr Pro Lys Gly Ser Val Glu Gln His
20 25 30
Thr Arg His Tyr Val Tyr Ala Ser Asp Asp Gly Phe Asp Pro Asn Phe
35 40 45
Ser Thr Gln Lys Ala Asp Thr Thr Arg Met Met Val Pro Phe Phe Arg
50 55 60
Gln Phe Trp Asp Met Gly Ala Lys Asp Lys Ala Thr Gly Lys Ser Arg
65 70 75 80
Ser Asp Val Gln Gln Arg Ile Gln Gln Phe His Ser Gln Glu Phe Leu
85 90 95
Asn Ser Leu Arg Gly Thr Thr Gln Phe Ala Gly Thr Asp Tyr Arg Ser
100 105 110
Lys Asp Leu Thr Pro Lys Lys Ser Arg Leu Leu Ala Asp Thr Ile Ser
115 120 125
Ala Val Tyr Leu Asp Gly Tyr Glu Gly Arg Gln
130 135
<210> 28
<211> 193
<212> PRT
<213> 痢疾志贺氏菌(Shigella dysenteriae)
<400> 28
Met Arg Tyr Leu Ala Thr Leu Leu Leu Ser Leu Ala Val Leu Ile Thr
1 5 10 15
Ala Gly Cys Gly Trp His Leu Arg Asp Thr Thr Gln Val Pro Ser Thr
20 25 30
Met Lys Val Met Ile Leu Asp Ser Gly Asp Pro Asn Gly Pro Leu Ser
35 40 45
Arg Ala Val Arg Asn Gln Leu Arg Leu Asn Gly Val Glu Leu Leu Asp
50 55 60
Lys Glu Thr Thr Arg Lys Asp Val Pro Ser Leu Arg Leu Gly Lys Val
65 70 75 80
Ser Ile Ala Lys Asp Thr Ala Ser Val Phe Arg Asn Gly Gln Thr Ala
85 90 95
Glu Tyr Gln Met Ile Met Thr Val Asn Ala Thr Val Leu Ile Pro Gly
100 105 110
Ser Asp Ile Tyr Pro Ile Ser Ala Lys Val Phe Arg Ser Phe Phe Asp
115 120 125
Asn Pro Gln Met Ala Leu Ala Lys Asp Asn Glu Gln Asp Met Ile Val
130 135 140
Lys Glu Met Tyr Asp Arg Ala Ala Glu Gln Leu Ile Arg Lys Leu Pro
145 150 155 160
Ser Ile Arg Ala Ala Asp Ile Arg Ser Asp Glu Glu Gln Thr Ser Thr
165 170 175
Thr Thr Asp Thr Pro Ala Thr Pro Ala Arg Val Ser Thr Thr Leu Gly
180 185 190
Asn
<210> 29
<211> 427
<212> PRT
<213> 宋内志贺氏菌
<400> 29
Met Asn Thr Phe Ser Val Ser Arg Leu Ala Leu Ala Leu Ala Phe Gly
1 5 10 15
Val Thr Leu Thr Ala Cys Ser Ser Thr Pro Pro Asp Gln Arg Pro Ser
20 25 30
Asp Gln Thr Ala Pro Gly Thr Ser Ser Arg Pro Ile Leu Ser Ala Lys
35 40 45
Glu Ala Gln Asn Phe Asp Ala Gln His Tyr Phe Ala Ser Leu Thr Pro
50 55 60
Gly Ala Ala Ala Trp Asn Pro Ser Pro Ile Thr Leu Pro Ala Gln Pro
65 70 75 80
Asp Phe Val Val Gly Pro Ala Gly Thr Gln Gly Val Thr His Thr Thr
85 90 95
Ile Gln Ala Ala Val Asp Ala Ala Ile Ile Lys Arg Thr Asn Lys Arg
100 105 110
Gln Tyr Ile Ala Val Met Pro Gly Glu Tyr Gln Gly Thr Val Tyr Val
115 120 125
Pro Ala Ala Pro Gly Gly Ile Thr Leu Tyr Gly Thr Gly Glu Lys Pro
130 135 140
Ile Asp Val Lys Ile Gly Leu Ser Leu Asp Gly Gly Met Ser Pro Ala
145 150 155 160
Asp Trp Arg His Asp Val Asn Pro Arg Gly Lys Tyr Met Pro Gly Lys
165 170 175
Pro Ala Trp Tyr Met Tyr Asp Ser Cys Gln Ser Lys Arg Ser Asp Ser
180 185 190
Ile Gly Val Leu Cys Ser Ala Val Phe Trp Ser Gln Asn Asn Gly Leu
195 200 205
Gln Leu Gln Asn Leu Thr Ile Glu Asn Thr Leu Gly Asp Ser Val Asp
210 215 220
Ala Gly Asn His Pro Ala Val Ala Leu Arg Thr Asp Gly Asp Gln Val
225 230 235 240
Gln Ile Asn Asn Val Asn Ile Leu Gly Arg Gln Asn Thr Phe Phe Val
245 250 255
Thr Asn Ser Gly Val Gln Asn Arg Leu Glu Thr Asn Arg Gln Pro Arg
260 265 270
Thr Leu Val Thr Asn Ser Tyr Ile Glu Gly Asp Val Asp Ile Val Ser
275 280 285
Gly Arg Gly Ala Val Val Phe Asp Asn Thr Glu Phe Arg Val Val Asn
290 295 300
Ser Arg Thr Gln Gln Glu Ala Tyr Val Phe Ala Pro Ala Thr Leu Ser
305 310 315 320
Asn Met Tyr Tyr Gly Phe Leu Ala Val Asn Ser Arg Phe Asn Ala Phe
325 330 335
Gly Asp Gly Val Ala Gln Leu Gly Arg Ser Leu Asp Val Asp Ala Asn
340 345 350
Thr Asn Gly Gln Val Val Ile Arg Asp Ser Ala Ile Asn Glu Gly Phe
355 360 365
Asn Thr Ala Lys Pro Trp Ala Asp Ala Val Ile Ser Asn Arg Pro Phe
370 375 380
Ala Gly Asn Thr Gly Ser Val Asp Asp Asn Asp Glu Ile Gln Arg Asn
385 390 395 400
Leu Asn Asp Thr Asn Tyr Asn Arg Met Trp Glu Tyr Asn Asn Arg Gly
405 410 415
Val Gly Ser Lys Val Val Ala Glu Ala Lys Lys
420 425
<210> 30
<211> 270
<212> PRT
<213> 福氏志贺氏菌
<400> 30
Met Lys Ser Leu Phe Lys Val Thr Leu Leu Ala Thr Thr Met Ala Val
1 5 10 15
Ala Leu His Ala Pro Ile Thr Phe Ala Ala Glu Ala Ala Lys Pro Ala
20 25 30
Thr Thr Ala Asp Ser Lys Ala Ser Phe Lys Asn Asp Asp Gln Lys Ser
35 40 45
Ala Tyr Ala Leu Gly Ala Ser Leu Gly Arg Tyr Met Glu Asn Ser Leu
50 55 60
Lys Glu Gln Glu Lys Leu Gly Ile Lys Leu Asp Lys Asp Gln Leu Ile
65 70 75 80
Ala Gly Val Gln Asp Ala Phe Ala Asp Lys Ser Lys Leu Ser Asp Gln
85 90 95
Glu Ile Glu Gln Thr Leu Gln Ala Phe Glu Ala Arg Val Lys Ser Ser
100 105 110
Ala Gln Ala Lys Met Glu Lys Asp Ala Ala Asp Asn Glu Ala Lys Gly
115 120 125
Lys Glu Tyr Arg Glu Lys Phe Ala Lys Glu Lys Gly Val Lys Thr Ser
130 135 140
Ser Thr Gly Leu Val Tyr Gln Val Val Glu Ala Gly Lys Gly Glu Ala
145 150 155 160
Pro Lys Asp Ser Asp Thr Val Val Val Asn Tyr Lys Gly Thr Leu Ile
165 170 175
Asp Gly Lys Glu Phe Asp Asn Ser Tyr Thr Arg Gly Glu Pro Leu Ser
180 185 190
Phe Arg Leu Asp Gly Val Ile Pro Gly Trp Thr Glu Gly Leu Lys Asn
195 200 205
Ile Lys Lys Gly Gly Lys Ile Lys Leu Val Ile Pro Pro Glu Leu Ala
210 215 220
Tyr Gly Lys Ala Gly Val Pro Gly Ile Pro Pro Asn Ser Thr Leu Val
225 230 235 240
Phe Asp Val Glu Leu Leu Asp Val Lys Pro Ala Pro Lys Ala Asp Ala
245 250 255
Lys Pro Glu Ala Asp Ala Lys Ala Ala Asp Ser Ala Lys Lys
260 265 270
<210> 31
<211> 260
<212> PRT
<213> 宋内志贺氏菌
<400> 31
Met Lys Lys Leu Val Leu Ser Leu Ser Leu Val Leu Ala Phe Ser Ser
1 5 10 15
Ala Thr Ala Ala Phe Ala Ala Ile Pro Gln Asn Ile Arg Ile Gly Thr
20 25 30
Asp Pro Thr Tyr Ala Pro Phe Glu Ser Lys Asn Ser Gln Gly Glu Leu
35 40 45
Val Gly Phe Asp Ile Asp Leu Ala Lys Glu Leu Cys Lys Arg Ile Asn
50 55 60
Thr Gln Cys Thr Phe Val Glu Asn Pro Leu Asp Ala Leu Ile Pro Ser
65 70 75 80
Leu Lys Ala Lys Lys Ile Asp Ala Ile Met Ser Ser Leu Ser Ile Thr
85 90 95
Glu Lys Arg Gln Gln Glu Ile Ala Phe Thr Asp Lys Leu Tyr Ala Ala
100 105 110
Asp Ser Arg Leu Val Val Ala Lys Asn Ser Asp Ile Gln Pro Thr Val
115 120 125
Glu Ser Leu Lys Gly Lys Arg Val Gly Val Leu Gln Gly Thr Thr Gln
130 135 140
Glu Thr Phe Gly Asn Glu His Trp Ala Pro Lys Gly Ile Glu Ile Val
145 150 155 160
Ser Tyr Gln Gly Gln Asp Asn Ile Tyr Ser Asp Leu Thr Ala Gly Arg
165 170 175
Ile Asp Ala Ala Phe Gln Asp Glu Val Ala Ala Ser Glu Gly Phe Leu
180 185 190
Lys Gln Pro Val Gly Lys Asp Tyr Lys Phe Gly Gly Pro Ser Val Lys
195 200 205
Asp Glu Lys Leu Phe Gly Val Gly Thr Gly Met Gly Leu Arg Lys Glu
210 215 220
Asp Asn Glu Leu Arg Glu Ala Leu Asn Lys Ala Phe Ala Glu Met Arg
225 230 235 240
Ala Asp Gly Thr Tyr Glu Lys Leu Ala Lys Lys Tyr Phe Asp Phe Asp
245 250 255
Val Tyr Gly Gly
260
<210> 32
<211> 474
<212> PRT
<213> 福氏志贺氏菌
<400> 32
Met Lys Lys Asn Thr Leu Ala Leu Ser Ala Leu Ala Leu Ser Leu Gly
1 5 10 15
Leu Ala Leu Ser Pro Leu Ser Ala Thr Ala Ala Glu Thr Ser Ser Ala
20 25 30
Thr Thr Ala Gln Gln Met Pro Ser Leu Ala Pro Met Leu Glu Lys Val
35 40 45
Met Pro Ser Val Val Ser Ile Asn Val Glu Gly Ser Thr Thr Val Asn
50 55 60
Thr Pro Arg Met Pro Arg Asn Phe Gln Gln Phe Phe Gly Asp Asp Ser
65 70 75 80
Pro Phe Cys Gln Glu Gly Ser Pro Phe Gln Ser Ser Pro Phe Cys Gln
85 90 95
Gly Gly Gln Gly Gly Asn Gly Gly Gly Gln Gln Gln Lys Phe Met Ala
100 105 110
Leu Gly Ser Gly Val Ile Ile Asp Ala Asp Lys Gly Tyr Val Val Thr
115 120 125
Asn Asn His Val Val Asp Asn Ala Thr Val Ile Lys Val Gln Leu Ser
130 135 140
Asp Gly Arg Lys Phe Asp Ala Lys Met Val Gly Lys Asp Pro Arg Ser
145 150 155 160
Asp Ile Ala Leu Ile Gln Ile Gln Asn Pro Lys Lys Leu Thr Ala Ile
165 170 175
Lys Met Ala Asp Ser Asp Ala Leu Arg Val Gly Asp Tyr Thr Val Ala
180 185 190
Ile Gly Asn Pro Phe Gly Leu Gly Glu Thr Val Thr Ser Gly Ile Val
195 200 205
Ser Ala Leu Gly Arg Ser Gly Leu Asn Ala Glu Asn Tyr Glu Asn Phe
210 215 220
Ile Gln Thr Asp Ala Ala Ile Asn Arg Gly Asn Ser Gly Gly Ala Leu
225 230 235 240
Val Asn Leu Asn Gly Glu Leu Ile Gly Ile Asn Thr Ala Ile Leu Ala
245 250 255
Pro Asp Gly Gly Asn Ile Gly Ile Gly Phe Ala Ile Pro Ser Asn Met
260 265 270
Val Lys Asn Leu Thr Ser Gln Met Val Glu Tyr Gly Gln Val Lys Arg
275 280 285
Gly Glu Leu Gly Ile Met Gly Thr Glu Leu Asn Ser Glu Leu Ala Lys
290 295 300
Ala Met Lys Val Asp Ala Gln Arg Gly Ala Phe Val Ser Gln Val Leu
305 310 315 320
Pro Asn Ser Ser Ala Ala Lys Ala Gly Ile Lys Ala Gly Asp Val Ile
325 330 335
Thr Ser Leu Asn Gly Lys Pro Ile Ser Ser Phe Ala Ala Leu Arg Ala
340 345 350
Gln Val Gly Thr Met Pro Val Gly Ser Lys Leu Thr Leu Gly Leu Leu
355 360 365
Arg Asp Gly Lys Gln Val Asn Val Asn Leu Glu Leu Gln Gln Ser Ser
370 375 380
Gln Asn Gln Val Asp Ser Ser Ser Ile Phe Asn Gly Ile Glu Gly Ala
385 390 395 400
Glu Met Ser Asn Lys Gly Lys Asp Gln Gly Val Val Val Asn Asn Val
405 410 415
Lys Thr Gly Thr Pro Ala Ala Gln Ile Gly Leu Lys Lys Gly Asp Val
420 425 430
Ile Ile Gly Ala Asn Gln Gln Ala Val Lys Asn Ile Ala Glu Leu Arg
435 440 445
Lys Val Leu Asp Ser Lys Pro Ser Val Leu Ala Leu Asn Ile Gln Arg
450 455 460
Gly Asp Ser Thr Ile Tyr Leu Leu Met Gln
465 470
<210> 33
<211> 430
<212> PRT
<213> 福氏志贺氏菌
<400> 33
Met Lys Gln Ala Leu Arg Val Ala Phe Gly Phe Leu Ile Leu Trp Ala
1 5 10 15
Ser Val Leu His Ala Glu Val Arg Ile Val Ile Asp Ser Gly Val Asp
20 25 30
Ser Gly Arg Pro Ile Gly Val Val Pro Phe Gln Trp Ala Gly Pro Gly
35 40 45
Ala Ala Pro Glu Asp Ile Gly Gly Ile Val Ala Ala Asp Leu Arg Asn
50 55 60
Ser Gly Lys Phe Asn Pro Leu Asp Arg Ala Arg Leu Pro Gln Gln Pro
65 70 75 80
Gly Ser Ala Gln Glu Val Gln Pro Ala Ala Trp Ser Ala Leu Gly Ile
85 90 95
Asp Ala Val Val Val Gly Gln Val Thr Pro Asn Pro Asp Gly Ser Tyr
100 105 110
Asn Val Ala Tyr Gln Leu Val Asp Thr Gly Gly Ala Pro Gly Thr Val
115 120 125
Leu Ala Gln Asn Ser Tyr Lys Val Asn Lys Gln Trp Leu Arg Tyr Ala
130 135 140
Gly His Thr Ala Ser Asp Glu Val Phe Glu Lys Leu Thr Gly Ile Lys
145 150 155 160
Gly Ala Phe Arg Thr Arg Ile Ala Tyr Val Val Gln Thr Asn Gly Gly
165 170 175
Gln Phe Pro Tyr Glu Leu Arg Val Ser Asp Tyr Asp Gly Tyr Asn Gln
180 185 190
Phe Val Val His Arg Ser Pro Gln Pro Leu Met Ser Pro Ala Trp Ser
195 200 205
Pro Asp Gly Ser Lys Leu Ala Tyr Val Thr Phe Glu Ser Gly Arg Ser
210 215 220
Ala Leu Val Ile Gln Thr Leu Ala Asn Gly Ala Val Arg Gln Val Ala
225 230 235 240
Ser Phe Pro Arg His Asn Gly Ala Pro Ala Phe Ser Pro Asp Gly Ser
245 250 255
Lys Leu Ala Phe Ala Leu Ser Lys Thr Gly Ser Leu Asn Leu Tyr Val
260 265 270
Met Asp Leu Ala Ser Gly Gln Ile Arg Gln Val Thr Asp Gly Arg Ser
275 280 285
Asn Asn Thr Glu Pro Thr Trp Phe Pro Asp Ser Gln Asn Leu Ala Phe
290 295 300
Thr Ser Asp Gln Ala Gly Arg Pro Gln Val Tyr Lys Val Asn Ile Asn
305 310 315 320
Gly Gly Ala Pro Gln Arg Ile Thr Trp Glu Gly Ser Gln Asn Gln Asp
325 330 335
Ala Asp Val Ser Ser Asp Gly Lys Phe Met Val Met Val Ser Ser Asn
340 345 350
Gly Gly Gln Gln His Ile Ala Lys Gln Asp Leu Ala Thr Gly Gly Val
355 360 365
Gln Val Leu Ser Ser Thr Phe Leu Asp Glu Thr Pro Ser Leu Ala Pro
370 375 380
Asn Gly Thr Met Val Ile Tyr Ser Ser Ser Gln Gly Met Gly Ser Val
385 390 395 400
Leu Asn Leu Val Ser Thr Asp Gly Arg Phe Lys Ala Arg Leu Pro Ala
405 410 415
Thr Asp Gly Gln Val Lys Phe Pro Ala Trp Ser Pro Tyr Leu
420 425 430
<210> 34
<211> 257
<212> PRT
<213> 福氏志贺氏菌
<400> 34
Met Ala Arg Lys Trp Leu Asn Leu Phe Ala Gly Ala Ala Leu Ser Phe
1 5 10 15
Ala Val Ala Gly Asn Ala Leu Ala Asp Glu Gly Lys Ile Thr Val Phe
20 25 30
Ala Ala Ala Ser Leu Thr Asn Ala Met Gln Asp Ile Ala Thr Gln Tyr
35 40 45
Lys Lys Glu Lys Gly Val Asp Val Val Ser Ser Phe Ala Ser Ser Ser
50 55 60
Thr Leu Ala Arg Gln Ile Glu Ala Gly Ala Pro Ala Asp Leu Phe Ile
65 70 75 80
Ser Ala Asp Gln Lys Trp Met Asp Tyr Ala Val Asp Lys Lys Ala Ile
85 90 95
Asp Thr Ala Thr Arg Gln Thr Leu Leu Gly Asn Ser Leu Val Val Val
100 105 110
Ala Pro Lys Ala Ser Glu Gln Lys Asp Phe Thr Ile Asp Ser Lys Thr
115 120 125
Asn Trp Thr Ser Leu Leu Asn Gly Gly Arg Leu Ala Val Gly Asp Pro
130 135 140
Glu His Val Pro Ala Gly Ile Tyr Ala Lys Glu Ala Leu Gln Lys Leu
145 150 155 160
Gly Ala Trp Asp Thr Leu Ser Pro Lys Leu Ala Pro Ala Glu Asp Val
165 170 175
Arg Gly Ala Leu Ala Leu Val Glu Arg Asn Glu Ala Pro Leu Gly Ile
180 185 190
Val Tyr Gly Ser Asp Ala Val Ala Ser Lys Gly Val Lys Val Val Ala
195 200 205
Thr Phe Pro Glu Asp Ser His Lys Lys Val Glu Tyr Pro Val Ala Val
210 215 220
Val Glu Gly His Asn Asn Ala Thr Val Lys Ala Phe Tyr Asp Tyr Leu
225 230 235 240
Lys Gly Pro Gln Ala Ala Glu Ile Phe Lys Arg Tyr Gly Phe Thr Thr
245 250 255
Lys
<210> 35
<211> 193
<212> PRT
<213> 福氏志贺氏菌
<400> 35
Met Phe Lys Ser Thr Leu Ala Ala Met Ala Ala Val Phe Ala Leu Ser
1 5 10 15
Ala Leu Ser Ala Leu Ser Pro Ala Ala Met Ala Ala Lys Gly Asp Pro
20 25 30
His Val Leu Leu Thr Thr Ser Ala Gly Asn Ile Glu Leu Glu Leu Asp
35 40 45
Lys Gln Lys Ala Pro Val Ser Val Gln Asn Phe Val Asp Tyr Val Asn
50 55 60
Ser Gly Phe Tyr Asn Asn Thr Thr Phe His Arg Val Ile Pro Gly Phe
65 70 75 80
Met Ile Gln Gly Gly Gly Phe Thr Glu Gln Met Gln Gln Lys Lys Pro
85 90 95
Asn Pro Pro Ile Lys Asn Glu Ala Asp Asn Gly Leu Arg Asn Thr Arg
100 105 110
Gly Thr Ile Ala Met Ala Arg Thr Ala Asp Lys Asp Ser Ala Thr Ser
115 120 125
Gln Phe Phe Ile Asn Val Ala Asp Asn Ala Phe Leu Asp His Gly Gln
130 135 140
Arg Asp Phe Gly Tyr Ala Val Phe Gly Lys Val Val Lys Gly Met Asp
145 150 155 160
Val Ala Asp Lys Ile Ser Gln Val Pro Thr His Asp Val Gly Pro Tyr
165 170 175
Gln Asn Val Pro Ser Lys Pro Val Val Ile Leu Ser Ala Lys Val Leu
180 185 190
Pro
<210> 36
<211> 428
<212> PRT
<213> 福氏志贺氏菌
<400> 36
Met Lys Asn Trp Lys Thr Leu Leu Leu Gly Ile Ala Met Ile Ala Asn
1 5 10 15
Thr Ser Phe Ala Ala Pro Gln Val Val Asp Lys Val Ala Ala Val Val
20 25 30
Asn Asn Gly Val Val Leu Glu Ser Asp Val Asp Gly Leu Met Gln Ser
35 40 45
Val Lys Leu Asn Ala Ala Gln Ala Arg Gln Gln Leu Pro Asp Asp Ala
50 55 60
Thr Leu Arg His Gln Ile Met Glu Arg Leu Ile Met Asp Gln Ile Ile
65 70 75 80
Leu Gln Met Gly Gln Lys Met Gly Val Lys Ile Ser Asp Glu Gln Leu
85 90 95
Asp Gln Ala Ile Ala Asn Ile Ala Lys Gln Asn Asn Met Thr Leu Asp
100 105 110
Gln Met Arg Ser Arg Leu Ala Tyr Asp Gly Leu Asn Tyr Asn Thr Tyr
115 120 125
Arg Asn Gln Ile Arg Lys Glu Met Ile Ile Ser Glu Val Arg Asn Asn
130 135 140
Glu Val Arg Arg Arg Ile Thr Ile Leu Pro Gln Glu Val Glu Ser Leu
145 150 155 160
Ala Gln Gln Val Gly Asn Gln Asn Asp Ala Ser Thr Glu Leu Asn Leu
165 170 175
Ser His Ile Leu Ile Pro Leu Pro Glu Asn Pro Thr Ser Asp Gln Val
180 185 190
Asn Glu Ala Glu Ser Gln Ala Arg Ala Ile Val Asp Gln Ala Arg Asn
195 200 205
Gly Ala Asp Phe Gly Lys Leu Ala Ile Ala His Ser Ala Asp Gln Gln
210 215 220
Ala Leu Asn Gly Gly Gln Met Gly Trp Gly Arg Ile Gln Glu Leu Pro
225 230 235 240
Gly Ile Phe Ala Gln Ala Leu Ser Thr Ala Lys Lys Gly Asp Ile Val
245 250 255
Gly Pro Ile Arg Ser Gly Val Gly Phe His Ile Leu Lys Val Asn Asp
260 265 270
Leu Arg Gly Glu Ser Lys Asn Ile Ser Val Thr Glu Val His Ala Arg
275 280 285
His Ile Leu Leu Lys Pro Ser Pro Ile Met Thr Asp Glu Gln Ala Arg
290 295 300
Val Lys Leu Glu Gln Ile Ala Ala Asp Ile Lys Ser Gly Lys Thr Thr
305 310 315 320
Phe Ala Ala Ala Ala Lys Glu Phe Ser Gln Asp Pro Gly Ser Ala Asn
325 330 335
Gln Gly Gly Asp Leu Gly Trp Ala Thr Pro Asp Ile Phe Asp Pro Ala
340 345 350
Phe Arg Asp Ala Leu Thr Arg Leu Asn Lys Gly Gln Met Ser Ala Pro
355 360 365
Val His Ser Ser Phe Gly Trp His Leu Ile Glu Leu Leu Asp Thr Arg
370 375 380
Asn Val Asp Lys Thr Asp Ala Ala Gln Lys Asp Arg Ala Tyr Arg Met
385 390 395 400
Leu Met Asn Arg Lys Phe Ser Glu Glu Ala Ala Ser Trp Met Gln Glu
405 410 415
Gln Arg Ala Ser Ala Tyr Val Lys Ile Leu Ser Asn
420 425
<210> 37
<211> 543
<212> PRT
<213> 福氏志贺氏菌
<400> 37
Met Thr Asn Ile Thr Lys Arg Ser Leu Val Ala Ala Gly Val Leu Ala
1 5 10 15
Ala Leu Met Ala Gly Asn Val Ala Leu Ala Ala Asp Val Pro Ala Gly
20 25 30
Val Thr Leu Ala Glu Lys Gln Thr Leu Val Arg Asn Asn Gly Ser Glu
35 40 45
Val Gln Ser Leu Asp Pro His Lys Ile Glu Gly Val Pro Glu Ser Asn
50 55 60
Ile Ser Arg Asp Leu Phe Glu Gly Leu Leu Val Ser Asp Leu Asp Gly
65 70 75 80
His Pro Ala Pro Gly Val Ala Glu Ser Trp Asp Asn Lys Asp Ala Lys
85 90 95
Val Trp Thr Phe His Leu Arg Lys Asp Ala Lys Trp Ser Asp Gly Thr
100 105 110
Pro Val Thr Ala Gln Asp Phe Val Tyr Ser Trp Gln Arg Ser Val Asp
115 120 125
Pro Asn Thr Ala Ser Pro Tyr Ala Ser Tyr Leu Gln Tyr Gly His Ile
130 135 140
Ala Gly Ile Asp Glu Ile Leu Glu Gly Lys Lys Pro Ile Thr Asp Leu
145 150 155 160
Gly Val Lys Ala Ile Asp Asp His Thr Leu Glu Val Thr Leu Ser Glu
165 170 175
Pro Val Pro Tyr Phe Tyr Lys Leu Leu Val His Pro Ser Thr Ser Pro
180 185 190
Val Pro Lys Ala Ala Ile Glu Lys Phe Gly Glu Lys Trp Thr Gln Pro
195 200 205
Gly Asn Ile Val Thr Asn Gly Ala Tyr Thr Leu Lys Asp Trp Val Val
210 215 220
Asn Glu Arg Ile Val Leu Glu Arg Ser Pro Thr Tyr Trp Asn Asn Ala
225 230 235 240
Lys Thr Val Ile Asn Gln Val Thr Tyr Leu Pro Ile Ala Ser Glu Val
245 250 255
Thr Asp Val Asn Arg Tyr Arg Ser Gly Glu Ile Asp Met Thr Tyr Asn
260 265 270
Asn Met Pro Ile Glu Leu Phe Gln Lys Leu Lys Lys Glu Ile Pro Asp
275 280 285
Glu Val His Val Asp Pro Tyr Leu Cys Thr Tyr Tyr Tyr Glu Ile Asn
290 295 300
Asn Gln Lys Pro Pro Phe Asn Asp Val Arg Val Arg Thr Ala Leu Lys
305 310 315 320
Leu Gly Met Asp Arg Asp Ile Ile Val Asn Lys Val Lys Ala Gln Gly
325 330 335
Asp Met Pro Ala Tyr Gly Tyr Thr Pro Pro Tyr Thr Asp Gly Ala Lys
340 345 350
Leu Thr Gln Pro Glu Trp Phe Gly Trp Ser Gln Glu Lys Arg Asn Glu
355 360 365
Glu Ala Lys Lys Leu Leu Ala Glu Ala Gly Tyr Thr Ala Asp Lys Pro
370 375 380
Leu Thr Ile Asn Leu Arg Tyr Asn Thr Ser Asp Leu His Lys Lys Leu
385 390 395 400
Ala Ile Ala Ala Ser Ser Leu Trp Lys Lys Asn Ile Gly Val Asn Val
405 410 415
Lys Leu Val Asn Gln Glu Trp Lys Thr Phe Leu Asp Thr Arg His Gln
420 425 430
Gly Thr Phe Asp Val Ala Arg Ala Gly Trp Cys Ala Asp Tyr Asn Glu
435 440 445
Pro Thr Ser Phe Leu Asn Thr Met Leu Ser Asn Ser Ser Met Asn Thr
450 455 460
Ala His Tyr Lys Ser Pro Ala Phe Asp Ser Ile Met Ala Glu Thr Leu
465 470 475 480
Lys Val Thr Asp Glu Ala Gln Arg Thr Ala Leu Tyr Thr Lys Ala Glu
485 490 495
Gln Gln Leu Asp Lys Asp Ser Ala Ile Val Pro Val Tyr Tyr Tyr Val
500 505 510
Asn Ala Arg Leu Val Lys Pro Trp Val Gly Gly Tyr Thr Gly Lys Asp
515 520 525
Pro Leu Asp Asn Thr Tyr Thr Arg Asn Met Tyr Ile Val Lys His
530 535 540
<210> 38
<211> 201
<212> PRT
<213> 福氏志贺氏菌
<400> 38
Met Thr Met Thr Arg Leu Lys Ile Ser Lys Thr Leu Leu Ala Val Met
1 5 10 15
Leu Thr Ser Ala Val Ala Thr Gly Ser Ala Tyr Ala Glu Asn Asn Ala
20 25 30
Gln Thr Thr Asn Glu Ser Ala Gly Gln Lys Val Asp Ser Ser Met Asn
35 40 45
Lys Val Gly Asn Phe Met Asp Asp Ser Ala Ile Thr Ala Lys Val Lys
50 55 60
Ala Ala Leu Val Asp His Asp Asn Ile Lys Ser Thr Asp Ile Ser Val
65 70 75 80
Lys Thr Asp Gln Lys Val Val Thr Leu Ser Gly Phe Val Glu Ser Gln
85 90 95
Ala Gln Ala Glu Glu Ala Val Lys Val Ala Lys Gly Val Glu Gly Val
100 105 110
Thr Ser Val Ser Asp Lys Leu His Val Arg Asp Ala Lys Glu Gly Ser
115 120 125
Val Lys Gly Tyr Ala Gly Asp Thr Ala Thr Thr Ser Glu Ile Lys Ala
130 135 140
Lys Leu Leu Ala Asp Asp Ile Val Pro Ser Arg Lys Val Lys Val Glu
145 150 155 160
Thr Thr Asp Gly Val Val Gln Leu Ser Gly Thr Val Asp Ser Gln Ala
165 170 175
Gln Ser Asp Arg Ala Glu Ser Ile Ala Lys Ala Val Asp Gly Val Lys
180 185 190
Ser Val Lys Asn Asp Leu Lys Thr Lys
195 200
<210> 39
<211> 243
<212> PRT
<213> 宋内志贺氏菌
<400> 39
Met Lys Lys Leu Val Leu Ala Ala Leu Leu Ala Ser Phe Thr Phe Gly
1 5 10 15
Ala Ser Ala Ala Glu Lys Ile Asn Phe Gly Val Ser Ala Thr Tyr Pro
20 25 30
Pro Phe Glu Ser Ile Gly Ala Asn Asn Glu Ile Val Gly Phe Asp Ile
35 40 45
Asp Leu Ala Lys Ala Leu Cys Lys Gln Met Gln Ala Glu Cys Thr Phe
50 55 60
Thr Asn His Ala Phe Asp Ser Leu Ile Pro Ser Leu Lys Phe Arg Lys
65 70 75 80
Tyr Asp Ser Val Ile Ser Gly Met Asp Ile Thr Pro Glu Arg Ser Lys
85 90 95
Gln Val Ser Phe Thr Thr Pro Tyr Tyr Glu Asn Ser Ala Val Val Ile
100 105 110
Ala Lys Lys Asp Thr Tyr Lys Thr Phe Ala Asp Leu Lys Gly Lys Arg
115 120 125
Ile Gly Met Glu Asn Gly Thr Thr His Gln Lys Tyr Ile Gln Asp Gln
130 135 140
His Pro Glu Val Lys Thr Val Ser Tyr Asp Ser Tyr Gln Asn Ala Phe
145 150 155 160
Ile Asp Leu Lys Asn Gly Arg Ile Asp Gly Val Phe Gly Asp Thr Ala
165 170 175
Val Val Asn Glu Trp Leu Lys Thr Asn Pro Gln Leu Gly Val Ala Thr
180 185 190
Glu Lys Val Thr Asp Pro Gln Tyr Phe Gly Thr Gly Leu Gly Ile Ala
195 200 205
Val Arg Pro Asp Asn Lys Ala Leu Leu Glu Lys Leu Asn Asn Ala Leu
210 215 220
Ala Ala Ile Lys Ala Asp Gly Thr Tyr Gln Lys Ile Asn Asp Gln Trp
225 230 235 240
Phe Pro Gln
<210> 40
<211> 550
<212> PRT
<213> 宋内志贺氏菌
<400> 40
Met Lys Leu Leu Gln Arg Gly Val Ala Leu Ala Leu Leu Thr Thr Phe
1 5 10 15
Thr Leu Ala Ser Glu Thr Ala Leu Ala Tyr Glu Gln Asp Lys Thr Tyr
20 25 30
Lys Ile Thr Val Leu His Thr Asn Asp His His Gly His Phe Trp Arg
35 40 45
Asn Glu Tyr Gly Glu Tyr Gly Leu Ala Ala Gln Lys Thr Leu Val Asp
50 55 60
Gly Ile Arg Lys Glu Val Ala Ala Glu Gly Gly Ser Val Leu Leu Leu
65 70 75 80
Ser Gly Gly Asp Ile Asn Thr Gly Val Pro Glu Ser Asp Leu Gln Asp
85 90 95
Ala Glu Pro Asp Phe Arg Gly Met Asn Leu Val Gly Tyr Asp Ala Met
100 105 110
Ala Ile Gly Asn His Glu Phe Asp Asn Pro Leu Thr Val Leu Arg Gln
115 120 125
Gln Glu Lys Trp Ala Lys Phe Pro Leu Leu Ser Ala Asn Ile Tyr Gln
130 135 140
Lys Ser Thr Gly Glu Arg Leu Phe Lys Pro Trp Ala Leu Phe Lys Arg
145 150 155 160
Gln Asp Leu Lys Ile Ala Val Ile Gly Leu Thr Thr Asp Asp Thr Ala
165 170 175
Lys Ile Gly Asn Pro Glu Tyr Phe Thr Asp Ile Glu Phe Arg Lys Pro
180 185 190
Ala Asp Glu Ala Lys Leu Val Ile Gln Glu Leu Gln Gln Thr Glu Lys
195 200 205
Pro Asp Ile Ile Ile Ala Ala Thr His Met Gly His Tyr Asp Asn Gly
210 215 220
Glu His Gly Ser Asn Ala Pro Gly Asp Val Glu Met Ala Arg Ala Leu
225 230 235 240
Pro Ala Gly Ser Leu Ala Met Ile Val Gly Gly His Ser Gln Asp Pro
245 250 255
Val Cys Met Ala Ala Glu Asn Lys Lys Gln Val Asp Tyr Val Pro Gly
260 265 270
Thr Pro Cys Lys Pro Asp Gln Gln Asn Gly Ile Trp Ile Val Gln Ala
275 280 285
His Glu Trp Gly Lys Tyr Val Gly Arg Ala Asp Phe Glu Phe Arg Asn
290 295 300
Gly Glu Met Lys Met Val Asn Tyr Gln Leu Ile Pro Val Asn Leu Lys
305 310 315 320
Lys Lys Val Thr Arg Glu Asp Gly Lys Ser Glu Arg Val Leu Tyr Thr
325 330 335
Pro Glu Ile Ala Glu Asn Gln Gln Met Ile Ser Leu Leu Ser Pro Phe
340 345 350
Gln Asn Lys Gly Lys Ala Gln Leu Glu Val Lys Ile Gly Glu Thr Asn
355 360 365
Gly Arg Leu Glu Gly Asp Arg Asp Lys Val Arg Phe Val Gln Thr Asn
370 375 380
Met Gly Arg Leu Ile Leu Ala Ala Gln Met Asp Arg Thr Gly Ala Asp
385 390 395 400
Phe Ala Val Met Ser Gly Gly Gly Ile Arg Asp Ser Ile Glu Ala Gly
405 410 415
Asp Ile Ser Tyr Lys Asn Val Leu Lys Val Gln Pro Phe Gly Asn Val
420 425 430
Val Val Tyr Ala Asp Met Ile Gly Lys Glu Val Ile Asp Tyr Leu Thr
435 440 445
Ala Val Ala Gln Met Lys Pro Asp Ser Gly Ala Tyr Pro Gln Phe Ala
450 455 460
Asn Val Ser Phe Val Ala Lys Asp Gly Lys Leu Asn Asp Leu Lys Ile
465 470 475 480
Lys Gly Glu Pro Val Asp Pro Ala Lys Thr Tyr Arg Met Ala Thr Leu
485 490 495
Asn Phe Asn Ala Thr Gly Gly Asp Gly Tyr Pro Arg Leu Asp Asn Lys
500 505 510
Pro Gly Tyr Val Asn Thr Gly Phe Ile Asp Ala Glu Val Leu Lys Ala
515 520 525
Tyr Ile Gln Lys Ser Ser Pro Leu Asp Val Ser Val Tyr Glu Pro Lys
530 535 540
Gly Glu Val Ser Trp Gln
545 550
<210> 41
<211> 266
<212> PRT
<213> 宋内志贺氏菌
<400> 41
Met Lys Leu Ala His Leu Gly Arg Gln Ala Leu Met Gly Val Met Ala
1 5 10 15
Val Ala Leu Val Ala Gly Met Ser Val Lys Ser Phe Ala Asp Glu Gly
20 25 30
Leu Leu Asn Lys Val Lys Glu Arg Gly Thr Leu Leu Val Gly Leu Glu
35 40 45
Gly Thr Tyr Pro Pro Phe Ser Phe Gln Gly Asp Asp Gly Lys Leu Thr
50 55 60
Gly Phe Glu Val Glu Phe Ala Gln Gln Leu Ala Lys His Leu Gly Val
65 70 75 80
Glu Ala Ser Leu Lys Pro Thr Lys Trp Asp Gly Met Leu Ala Ser Leu
85 90 95
Asp Ser Lys Arg Ile Asp Val Val Ile Asn Gln Val Thr Ile Ser Asp
100 105 110
Glu Arg Lys Lys Lys Tyr Asp Phe Ser Thr Pro Tyr Thr Ile Ser Gly
115 120 125
Ile Gln Ala Leu Val Lys Lys Gly Asn Glu Gly Thr Ile Lys Thr Ala
130 135 140
Asp Asp Leu Lys Gly Lys Lys Val Gly Val Gly Leu Gly Thr Asn Tyr
145 150 155 160
Glu Glu Trp Leu Arg Gln Asn Val Gln Gly Val Asp Val Arg Thr Tyr
165 170 175
Asp Asp Asp Pro Thr Lys Tyr Gln Asp Leu Arg Val Gly Arg Ile Asp
180 185 190
Ala Ile Leu Val Asp Arg Leu Ala Ala Leu Asp Leu Val Lys Lys Thr
195 200 205
Asn Asp Thr Leu Ala Val Thr Gly Glu Ala Phe Ser Arg Gln Glu Ser
210 215 220
Gly Val Ala Leu Arg Lys Gly Asn Glu Asp Leu Leu Lys Ala Val Asn
225 230 235 240
Asp Ala Ile Ala Glu Met Gln Lys Asp Gly Thr Leu Gln Ala Leu Ser
245 250 255
Glu Lys Trp Phe Gly Ala Asp Val Thr Lys
260 265
<210> 42
<211> 508
<212> PRT
<213> 福氏志贺氏菌
<400> 42
Met Arg Trp Leu Ser Ala Ala Val Met Leu Thr Leu Tyr Thr Ser Ser
1 5 10 15
Ser Trp Ala Phe Ser Ile Asp Asp Val Ala Lys Gln Ala Gln Ser Leu
20 25 30
Ala Gly Lys Gly Tyr Glu Ala Pro Lys Ser Asn Leu Pro Ser Val Phe
35 40 45
Arg Asp Met Lys Tyr Ala Asp Tyr Gln Gln Ile Gln Phe Asn His Asp
50 55 60
Lys Ala Tyr Trp Asn Asn Leu Lys Thr Pro Phe Lys Leu Glu Phe Tyr
65 70 75 80
His Gln Gly Met Tyr Phe Asp Thr Pro Val Lys Ile Asn Glu Val Thr
85 90 95
Ala Thr Ala Val Lys Arg Ile Lys Tyr Ser Pro Asp Tyr Phe Thr Phe
100 105 110
Gly Asp Val Gln His Asp Lys Asp Thr Val Lys Asp Leu Gly Phe Ala
115 120 125
Gly Phe Lys Val Leu Tyr Pro Ile Asn Ser Lys Asp Lys Asn Asp Glu
130 135 140
Ile Val Ser Met Leu Gly Ala Ser Tyr Phe Arg Val Ile Gly Ala Gly
145 150 155 160
Gln Val Tyr Gly Leu Ser Ala Arg Gly Leu Ala Ile Asp Thr Ala Leu
165 170 175
Pro Ser Gly Glu Glu Phe Pro Arg Phe Lys Glu Phe Trp Ile Glu Arg
180 185 190
Pro Lys Pro Thr Asp Lys Arg Leu Thr Ile Tyr Ala Leu Leu Asp Ser
195 200 205
Pro Arg Ala Thr Gly Ala Tyr Lys Phe Val Val Met Pro Gly Arg Asp
210 215 220
Thr Val Val Asp Val Gln Ser Lys Ile Tyr Leu Arg Asp Lys Val Gly
225 230 235 240
Lys Leu Gly Val Ala Pro Leu Thr Ser Met Phe Leu Phe Gly Pro Asn
245 250 255
Gln Pro Ser Pro Ala Asn Asn Tyr Arg Pro Glu Leu His Asp Ser Asn
260 265 270
Gly Leu Ser Ile His Ala Gly Asn Gly Glu Trp Ile Trp Arg Pro Leu
275 280 285
Asn Asn Pro Lys His Leu Ala Val Ser Ser Phe Ser Met Glu Asn Pro
290 295 300
Arg Gly Phe Gly Leu Leu Gln Arg Gly Arg Asp Phe Ser Arg Phe Glu
305 310 315 320
Asp Leu Asp Asp Arg Tyr Asp Leu Arg Pro Ser Ala Trp Val Thr Pro
325 330 335
Lys Gly Glu Trp Gly Lys Gly Ser Val Glu Leu Val Glu Ile Pro Thr
340 345 350
Asn Asp Glu Thr Asn Asp Asn Ile Val Ala Tyr Trp Thr Pro Asp Gln
355 360 365
Leu Pro Glu Pro Gly Lys Glu Met Asn Phe Lys Tyr Thr Ile Thr Phe
370 375 380
Ser Arg Asp Glu Asp Lys Leu His Ala Pro Asp Asn Ala Trp Val Gln
385 390 395 400
Gln Thr Arg Arg Ser Thr Gly Asp Val Lys Gln Ser Asn Leu Ile Arg
405 410 415
Gln Pro Asp Gly Thr Ile Ala Phe Val Val Asp Phe Thr Gly Ala Glu
420 425 430
Met Lys Lys Leu Pro Glu Asp Thr Pro Val Thr Ala Gln Thr Ser Ile
435 440 445
Gly Asp Asn Gly Glu Ile Val Glu Ser Thr Val Arg Tyr Asn Pro Val
450 455 460
Thr Lys Gly Trp Arg Leu Val Met Arg Val Lys Val Lys Asp Ala Lys
465 470 475 480
Lys Thr Thr Glu Met Arg Ala Ala Leu Val Asn Ala Asp Gln Thr Leu
485 490 495
Ser Glu Thr Trp Ser Tyr Gln Leu Pro Ala Asn Glu
500 505
<210> 43
<211> 338
<212> PRT
<213> 宋内志贺氏菌
<400> 43
Met Ala Val Asn Leu Leu Lys Lys Asn Ser Leu Ala Leu Val Ala Ser
1 5 10 15
Leu Leu Leu Ala Gly His Val Gln Ala Thr Glu Leu Leu Asn Ser Ser
20 25 30
Tyr Asp Val Ser Arg Glu Leu Phe Ala Ala Leu Asn Pro Pro Phe Glu
35 40 45
Gln Gln Trp Thr Lys Glu Asn Gly Gly Asp Lys Leu Thr Ile Lys Gln
50 55 60
Ser His Ala Gly Ser Ser Lys Gln Ala Leu Ala Ile Leu Gln Gly Leu
65 70 75 80
Lys Ala Asp Val Val Thr Tyr Asn Gln Val Thr Asp Val Gln Ile Leu
85 90 95
His Asp Lys Gly Lys Leu Ile Pro Ala Asp Trp Gln Thr Arg Leu Pro
100 105 110
Asn Asn Ser Ser Pro Phe Tyr Ser Thr Met Gly Phe Leu Val Arg Lys
115 120 125
Gly Asn Pro Lys Asn Ile His Asp Trp Asn Asp Leu Val Arg Ser Asp
130 135 140
Val Lys Leu Ile Phe Pro Asn Pro Lys Thr Ser Gly Asn Ala Arg Tyr
145 150 155 160
Thr Tyr Leu Ala Ala Trp Gly Ala Ala Asp Lys Ala Asp Gly Gly Asp
165 170 175
Lys Ala Lys Thr Glu Gln Phe Met Thr Gln Phe Leu Lys Asn Val Glu
180 185 190
Val Phe Asp Thr Gly Gly Arg Gly Ala Thr Thr Thr Phe Ala Glu Arg
195 200 205
Gly Leu Gly Asp Val Leu Ile Ser Phe Glu Ser Glu Val Asn Asn Ile
210 215 220
Arg Lys Gln Tyr Glu Ala Gln Gly Phe Glu Val Val Ile Pro Lys Thr
225 230 235 240
Asn Ile Leu Ala Glu Phe Pro Val Ala Trp Val Asp Lys Asn Val Gln
245 250 255
Ala Asn Gly Thr Glu Lys Ala Ala Lys Ala Tyr Leu Asn Trp Leu Tyr
260 265 270
Ser Pro Gln Ala Gln Thr Ile Ile Thr Asp Tyr Tyr Tyr Arg Val Asn
275 280 285
Asn Pro Glu Val Met Asp Lys Leu Lys Asp Lys Phe Pro Gln Thr Glu
290 295 300
Leu Phe Arg Val Glu Asp Lys Phe Gly Ser Trp Pro Glu Val Met Lys
305 310 315 320
Thr His Phe Thr Ser Gly Gly Glu Leu Asp Lys Leu Leu Ala Ala Gly
325 330 335
Arg Asn
<210> 44
<211> 191
<212> PRT
<213> 福氏志贺氏菌
<400> 44
Met Lys Ala Leu Ser Pro Ile Ala Val Leu Ile Ser Ala Leu Leu Leu
1 5 10 15
Gln Gly Cys Val Ala Ala Ala Val Val Gly Thr Ala Ala Val Gly Thr
20 25 30
Lys Ala Ala Thr Asp Pro Arg Ser Val Gly Thr Gln Val Asp Asp Gly
35 40 45
Thr Leu Glu Val Arg Val Asn Ser Ala Leu Ser Lys Asp Glu Gln Ile
50 55 60
Lys Lys Glu Thr Arg Ile Asn Val Thr Ala Tyr Gln Gly Lys Val Leu
65 70 75 80
Leu Val Gly Gln Ser Pro Asn Ala Glu Leu Ser Ala Arg Ala Lys Gln
85 90 95
Ile Ala Met Gly Val Asp Gly Ala Asn Glu Val Tyr Asn Glu Ile Arg
100 105 110
Gln Gly Gln Pro Ile Gly Leu Gly Glu Ala Ser Asn Asp Thr Trp Ile
115 120 125
Thr Thr Lys Val Arg Ser Gln Leu Leu Thr Ser Asp Leu Val Lys Ser
130 135 140
Ser Asn Val Lys Val Thr Thr Glu Asn Gly Glu Val Phe Leu Met Gly
145 150 155 160
Leu Val Thr Glu Arg Glu Ala Lys Ala Ala Ala Asp Ile Ala Ser Arg
165 170 175
Val Ser Gly Val Lys Arg Val Thr Thr Ala Phe Thr Phe Ile Lys
180 185 190
<210> 45
<211> 353
<212> PRT
<213> 宋内志贺氏菌
<400> 45
Met His Leu Arg His Leu Phe Ser Ser Arg Leu Arg Gly Ser Leu Leu
1 5 10 15
Leu Gly Ser Leu Leu Val Ala Ser Ser Phe Ser Thr Gln Ala Ala Glu
20 25 30
Glu Met Leu Arg Lys Ala Val Gly Lys Gly Ala Tyr Glu Met Ala Tyr
35 40 45
Ser Gln Gln Glu Asn Ala Leu Trp Leu Ala Thr Ser Gln Ser Arg Lys
50 55 60
Leu Asp Lys Gly Gly Val Val Tyr Arg Leu Asp Pro Val Thr Leu Glu
65 70 75 80
Val Thr Gln Ala Ile His Asn Asp Leu Lys Pro Phe Gly Val Thr Ile
85 90 95
Asn Asn Thr Thr Gln Thr Leu Trp Phe Gly Asn Thr Val Asn Ser Ala
100 105 110
Val Thr Ala Ile Asp Ala Lys Thr Gly Glu Val Lys Gly Arg Leu Val
115 120 125
Leu Asp Asp Arg Lys Arg Thr Glu Glu Val Arg Pro Leu Gln Pro Arg
130 135 140
Glu Leu Val Ala Asp Asp Ala Thr Asn Thr Val Tyr Ile Ser Gly Ile
145 150 155 160
Gly Lys Glu Ser Val Ile Trp Val Val Asp Gly Glu Asn Ile Lys Leu
165 170 175
Lys Thr Ala Ile Gln Asn Thr Gly Lys Met Ser Thr Gly Leu Ala Leu
180 185 190
Asp Ser Lys Gly Lys Arg Leu Tyr Thr Thr Asn Ala Asp Gly Glu Leu
195 200 205
Ile Thr Ile Asp Thr Ala Asp Asn Lys Ile Leu Ser Arg Lys Lys Leu
210 215 220
Leu Asp Asp Gly Lys Glu His Phe Phe Ile Asn Ile Ser Leu Asp Thr
225 230 235 240
Ala Arg Gln Arg Ala Phe Ile Thr Asp Ser Lys Ala Ala Glu Val Leu
245 250 255
Val Val Asp Thr Arg Asn Gly Asn Ile Leu Ala Lys Val Ala Ala Pro
260 265 270
Glu Ser Leu Ala Val Leu Phe Asn Pro Ala Arg Asn Glu Ala Tyr Val
275 280 285
Thr His Arg Gln Ala Gly Lys Val Ser Val Ile Asp Ala Lys Ser Tyr
290 295 300
Lys Val Val Lys Thr Phe Asp Thr Pro Thr His Pro Asn Ser Leu Ala
305 310 315 320
Leu Ser Ala Asp Gly Lys Thr Leu Tyr Val Ser Val Lys Gln Lys Ser
325 330 335
Thr Lys Gln Gln Glu Ala Thr Gln Pro Asp Asp Val Ile Arg Ile Ala
340 345 350
Leu
<210> 46
<211> 502
<212> PRT
<213> 宋内志贺氏菌
<400> 46
Met Asn Lys Ser Leu Val Ala Val Gly Val Ile Val Ala Leu Gly Val
1 5 10 15
Val Trp Thr Gly Gly Ala Trp Tyr Thr Gly Lys Lys Ile Glu Thr His
20 25 30
Leu Glu Asp Met Val Ala Gln Ala Asn Ala Gln Leu Lys Leu Thr Ala
35 40 45
Pro Glu Ser Asn Leu Glu Val Ser Tyr Gln Asn Tyr His Arg Gly Val
50 55 60
Phe Ser Ser Gln Leu Gln Leu Leu Val Lys Pro Ile Ala Gly Lys Glu
65 70 75 80
Asn Pro Trp Ile Lys Ser Gly Gln Ser Val Ile Phe Asn Glu Ser Val
85 90 95
Asp His Gly Pro Phe Pro Leu Ala Gln Leu Lys Lys Leu Asn Leu Ile
100 105 110
Pro Ser Met Ala Ser Ile Gln Thr Thr Leu Val Asn Asn Glu Val Ser
115 120 125
Lys Pro Leu Phe Asp Met Ala Lys Gly Glu Thr Pro Phe Glu Ile Asn
130 135 140
Ser Arg Ile Gly Tyr Ser Gly Asp Ser Ser Ser Asp Ile Ser Leu Lys
145 150 155 160
Pro Leu Asn Tyr Glu Gln Lys Asp Glu Lys Val Ala Phe Ser Gly Gly
165 170 175
Glu Phe Gln Leu Asn Ala Asp Arg Asp Gly Lys Ala Ile Ser Leu Ser
180 185 190
Gly Glu Ala Gln Ser Gly Arg Ile Asp Ala Val Asn Glu Tyr Asn Gln
195 200 205
Lys Val Gln Leu Thr Phe Asn Asn Leu Lys Thr Asp Gly Ser Ser Thr
210 215 220
Leu Ala Ser Phe Gly Glu Arg Val Gly Asn Gln Lys Leu Ser Leu Glu
225 230 235 240
Lys Met Thr Ile Ser Val Glu Gly Lys Glu Leu Ala Leu Leu Glu Gly
245 250 255
Met Glu Ile Ser Gly Lys Ser Asp Leu Val Asn Asp Gly Lys Thr Ile
260 265 270
Asn Ser Gln Leu Asp Tyr Ser Leu Asn Ser Leu Lys Val Gln Asn Gln
275 280 285
Asp Leu Gly Ser Gly Lys Leu Thr Leu Lys Val Gly Gln Ile Asp Gly
290 295 300
Glu Ala Trp His Gln Phe Ser Gln Gln Tyr Asn Ala Gln Thr Gln Ala
305 310 315 320
Leu Leu Ala Gln Pro Glu Ile Ala Asn Asn Pro Glu Leu Tyr Gln Glu
325 330 335
Lys Val Thr Glu Ala Phe Phe Ser Ala Leu Pro Leu Met Leu Lys Gly
340 345 350
Asp Pro Val Ile Thr Ile Ala Pro Leu Ser Trp Lys Asn Ser Gln Gly
355 360 365
Glu Ser Ala Leu Asn Leu Ser Leu Phe Leu Lys Asp Pro Ala Thr Thr
370 375 380
Lys Glu Ala Pro Gln Thr Leu Ala Gln Glu Val Asp Arg Ser Val Lys
385 390 395 400
Ser Leu Asp Ala Lys Leu Thr Ile Pro Val Asp Met Ala Thr Glu Phe
405 410 415
Met Thr Gln Val Ala Lys Leu Glu Gly Tyr Gln Glu Asp Gln Ala Lys
420 425 430
Lys Leu Ala Lys Gln Gln Val Glu Gly Ala Ser Ala Met Gly Gln Met
435 440 445
Phe Arg Leu Thr Thr Leu Gln Asp Asn Thr Ile Thr Thr Ser Leu Gln
450 455 460
Tyr Thr Asn Gly Gln Ile Thr Leu Asn Gly Gln Lys Met Pro Leu Glu
465 470 475 480
Asp Phe Val Gly Met Phe Ala Met Pro Ala Leu Asn Val Pro Ala Val
485 490 495
Pro Ala Ile Pro Gln Gln
500
<210> 47
<211> 269
<212> PRT
<213> 福氏志贺氏菌
<400> 47
Met Glu Lys Lys Ile Gly Phe Ile Gly Cys Gly Asn Met Gly Lys Ala
1 5 10 15
Ile Leu Gly Gly Leu Ile Ala Ser Gly Gln Val Leu Pro Gly Gln Ile
20 25 30
Trp Val Tyr Thr Pro Ser Pro Asp Lys Val Ala Ala Leu His Asp Gln
35 40 45
Phe Gly Ile Asn Ala Ala Glu Ser Ala Gln Glu Val Ala Gln Ile Ala
50 55 60
Asp Ile Ile Phe Ala Ala Val Lys Pro Gly Ile Met Ile Lys Val Leu
65 70 75 80
Ser Glu Ile Thr Ser Ser Leu Asn Lys Asp Ser Leu Val Val Ser Ile
85 90 95
Ala Ala Gly Val Thr Leu Asp Gln Leu Ala Arg Ala Leu Gly His Asp
100 105 110
Arg Lys Ile Ile Arg Ala Met Pro Asn Thr Pro Ala Leu Val Asn Ala
115 120 125
Gly Met Thr Ser Val Thr Pro Asn Ala Leu Val Thr Pro Glu Asp Thr
130 135 140
Ala Asp Val Leu Asn Ile Phe Arg Cys Phe Gly Glu Ala Glu Val Ile
145 150 155 160
Ala Glu Pro Met Ile His Pro Val Val Gly Val Ser Gly Ser Ser Pro
165 170 175
Ala Tyr Val Phe Met Phe Ile Glu Ala Met Ala Asp Ala Ala Val Leu
180 185 190
Gly Gly Met Pro Arg Ala Gln Ala Tyr Lys Phe Ala Ala Gln Ala Val
195 200 205
Met Gly Ser Ala Lys Met Val Leu Glu Thr Gly Glu His Pro Gly Ala
210 215 220
Leu Lys Asp Met Val Cys Ser Pro Gly Gly Thr Thr Ile Glu Ala Val
225 230 235 240
Arg Val Leu Glu Glu Lys Gly Phe Arg Ala Ala Val Ile Glu Ala Met
245 250 255
Thr Lys Cys Met Glu Lys Ser Glu Lys Leu Ser Lys Ser
260 265
<210> 48
<211> 307
<212> PRT
<213> 福氏志贺氏菌
<400> 48
Met Arg Ser Arg Phe Ser Ala Phe Tyr Pro Lys Ala Asn Tyr Gly Leu
1 5 10 15
Gln Gly Ser Gln Pro Ser Asp Val Arg Ala His Asn Arg Ala Ala Asn
20 25 30
Gly Ala Cys Asp Glu Tyr Lys Gln Leu Lys Val Leu Ser Met Gly Arg
35 40 45
Gln Lys Ala Val Ile Lys Ala Arg Arg Glu Ala Lys Arg Val Leu Arg
50 55 60
Arg Asp Ser Arg Ser His Lys Gln Arg Glu Glu Glu Ser Val Thr Ser
65 70 75 80
Leu Val Gln Met Ser Gly Val Glu Ala Ile Gly Met Ala Arg Asp Ser
85 90 95
Arg Asp Thr Ser Pro Ile Leu Ala Arg Asn Glu Ala Gln Leu His Tyr
100 105 110
Leu Gln Ala Ile Glu Ser Lys Gln Leu Ile Phe Ala Thr Gly Glu Ala
115 120 125
Gly Cys Gly Lys Thr Trp Ile Ser Ala Ala Lys Ala Ala Glu Ala Leu
130 135 140
Ile His Lys Asp Val Asp Arg Ile Ile Val Thr Arg Pro Val Leu Gln
145 150 155 160
Ala Asp Glu Asp Leu Gly Phe Leu Pro Gly Asp Ile Ala Glu Lys Phe
165 170 175
Ala Pro Tyr Phe Arg Pro Val Tyr Asp Leu Leu Val Arg Arg Leu Gly
180 185 190
Ala Ser Phe Met Gln Tyr Cys Leu Arg Pro Glu Ile Gly Lys Val Glu
195 200 205
Thr Ala Pro Phe Ala Tyr Met Arg Gly Arg Thr Phe Glu Asn Ala Val
210 215 220
Val Ile Leu Asp Glu Ala Gln Asn Val Thr Ala Ala Gln Met Lys Met
225 230 235 240
Phe Leu Thr Arg Leu Gly Glu Asn Val Thr Val Ile Val Asn Gly Asp
245 250 255
Ile Thr Gln Cys Asp Leu Pro Arg Gly Val Cys Ser Gly Leu Ser Asp
260 265 270
Ala Leu Glu Arg Phe Glu Glu Asp Glu Met Val Gly Ile Val Arg Phe
275 280 285
Gly Lys Glu Asp Cys Val Arg Ser Ala Leu Cys Gln Arg Thr Leu His
290 295 300
Ala Tyr Ser
305
<210> 49
<211> 235
<212> PRT
<213> 福氏志贺氏菌
<400> 49
Met Gly Ile Ser Gly Met Ala Ala Gln Ala Asn Glu Leu Pro Asp Gly
1 5 10 15
Pro His Ile Val Thr Ser Gly Thr Ala Ser Val Asp Ala Val Pro Asp
20 25 30
Ile Ala Thr Leu Ala Ile Glu Val Asn Val Ala Ala Lys Asp Ala Ala
35 40 45
Thr Ala Lys Lys Gln Ala Asp Glu Arg Val Ala Gln Tyr Ile Ser Phe
50 55 60
Leu Glu Leu Asn Gln Ile Ala Lys Lys Asp Ile Ser Ser Ala Asn Leu
65 70 75 80
Arg Thr Gln Pro Asp Tyr Asp Tyr Gln Asp Gly Lys Ser Ile Leu Lys
85 90 95
Gly Tyr Arg Ala Val Arg Thr Val Glu Val Thr Leu Arg Gln Leu Asp
100 105 110
Lys Leu Asn Ser Leu Leu Asp Gly Ala Leu Lys Ala Gly Leu Asn Glu
115 120 125
Ile Arg Ser Val Ser Leu Gly Val Ala Gln Pro Asp Ala Tyr Lys Asp
130 135 140
Lys Ala Arg Lys Ala Ala Ile Asp Asn Ala Ile His Gln Ala Gln Glu
145 150 155 160
Leu Ala Asn Gly Phe His Arg Lys Leu Gly Pro Val Tyr Ser Val Arg
165 170 175
Tyr His Val Ser Asn Tyr Gln Pro Ser Pro Met Val Arg Met Met Lys
180 185 190
Ala Asp Ala Ala Pro Val Ser Ala Gln Glu Thr Tyr Glu Gln Ala Ala
195 200 205
Ile Gln Phe Asp Asp Gln Val Asp Val Val Phe Gln Leu Glu Pro Val
210 215 220
Asp Gln Gln Pro Ala Lys Thr Pro Ala Ala Gln
225 230 235
<210> 50
<211> 314
<212> PRT
<213> 宋内志贺氏菌
<400> 50
Met Lys Leu Lys Asn Thr Leu Leu Ala Ser Ala Leu Leu Ser Ala Thr
1 5 10 15
Ala Phe Ser Val Asn Ala Ala Thr Glu Leu Thr Pro Glu Gln Ala Ala
20 25 30
Ala Val Lys Pro Phe Asp Arg Val Val Val Thr Gly Arg Phe Asn Ala
35 40 45
Ile Gly Glu Ala Val Lys Ala Val Ser Arg Arg Ala Asp Lys Glu Gly
50 55 60
Ala Ala Ser Phe Tyr Val Val Asp Thr Ser Asp Phe Gly Asn Ser Gly
65 70 75 80
Asn Trp Arg Val Val Ala Asp Leu Tyr Lys Ala Asp Ala Glu Lys Ala
85 90 95
Glu Glu Thr Ser Asn Arg Val Ile Asn Gly Val Val Glu Leu Pro Lys
100 105 110
Asp Gln Ala Val Leu Ile Glu Pro Phe Asp Thr Val Thr Val Gln Gly
115 120 125
Phe Tyr Arg Ser Gln Pro Glu Val Asn Asp Ala Ile Thr Lys Ala Ala
130 135 140
Lys Ala Lys Gly Ala Tyr Ser Phe Tyr Ile Val Arg Gln Ile Asp Ala
145 150 155 160
Asn Gln Gly Gly Asn Gln Arg Ile Thr Ala Phe Ile Tyr Lys Lys Asp
165 170 175
Ala Lys Lys Arg Ile Val Gln Ser Pro Asp Val Ile Pro Ala Asp Ser
180 185 190
Glu Ala Gly Arg Ala Ala Leu Ala Ala Gly Gly Glu Ala Ala Lys Lys
195 200 205
Val Glu Ile Leu Gly Val Ala Thr Thr Ala Ser Pro Ser Ser Glu Val
210 215 220
Gly Arg Phe Phe Glu Thr Gln Ser Ser Lys Gly Gly Arg Tyr Thr Val
225 230 235 240
Met Leu Pro Asp Gly Thr Lys Val Glu Glu Leu Asn Lys Ala Thr Ala
245 250 255
Ala Met Met Val Pro Phe Asp Ser Ile Lys Phe Ser Gly Asn Tyr Gly
260 265 270
Asn Met Thr Glu Val Ser Tyr Gln Val Ala Lys Arg Ala Ala Lys Lys
275 280 285
Gly Ala Lys Tyr Tyr His Ile Thr Arg Gln Trp Gln Glu Arg Gly Asn
290 295 300
Asn Leu Thr Val Ser Ala Asp Leu Tyr Lys
305 310
<210> 51
<211> 211
<212> PRT
<213> 宋内志贺氏菌
<400> 51
Met Phe Lys Arg Leu Met Met Val Ala Leu Leu Val Ile Ala Pro Leu
1 5 10 15
Ser Ala Ala Thr Ala Ala Asp Gln Thr Asn Pro Tyr Lys Leu Met Asp
20 25 30
Glu Ala Ala Gln Lys Thr Phe Asp Arg Leu Lys Asn Glu Gln Pro Gln
35 40 45
Ile Arg Ala Asn Pro Asp Tyr Leu Arg Thr Ile Val Asp Gln Glu Leu
50 55 60
Leu Pro Tyr Val Gln Val Lys Tyr Ala Gly Ala Leu Val Leu Gly Gln
65 70 75 80
Tyr Tyr Lys Ser Ala Thr Pro Ala Gln Arg Glu Ala Tyr Phe Ala Ala
85 90 95
Phe Arg Glu Tyr Leu Lys Gln Ala Tyr Gly Gln Ala Leu Ala Met Tyr
100 105 110
His Gly Gln Thr Tyr Gln Ile Ala Pro Glu Gln Pro Leu Gly Asp Lys
115 120 125
Thr Ile Val Pro Ile Arg Val Thr Ile Ile Asp Pro Asn Gly Arg Pro
130 135 140
Pro Val Arg Leu Asp Phe Gln Trp Arg Lys Asn Ser Gln Thr Gly Asn
145 150 155 160
Trp Gln Ala Tyr Asp Met Ile Ala Glu Gly Val Ser Met Ile Thr Thr
165 170 175
Lys Gln Asn Glu Trp Gly Thr Leu Leu Arg Thr Lys Gly Ile Asp Gly
180 185 190
Leu Thr Ala Gln Leu Lys Ser Ile Ser Gln Gln Lys Ile Thr Leu Glu
195 200 205
Glu Lys Lys
210
<210> 52
<211> 548
<212> PRT
<213> 福氏志贺氏菌
<400> 52
Met Ala Ala Lys Asp Val Lys Phe Gly Asn Asp Ala Arg Val Lys Met
1 5 10 15
Leu Arg Gly Val Asn Val Leu Ala Asp Ala Val Lys Val Thr Leu Gly
20 25 30
Pro Lys Gly Arg Asn Val Val Leu Asp Lys Ser Phe Gly Ala Pro Thr
35 40 45
Ile Thr Lys Asp Gly Val Ser Val Ala Arg Glu Ile Glu Leu Glu Asp
50 55 60
Lys Phe Glu Asn Met Gly Ala Gln Met Val Lys Glu Val Ala Ser Lys
65 70 75 80
Ala Asn Asp Ala Ala Gly Asp Gly Thr Thr Thr Ala Thr Val Leu Ala
85 90 95
Gln Ala Ile Ile Thr Glu Gly Leu Lys Ala Val Ala Ala Gly Met Asn
100 105 110
Pro Met Asp Leu Lys Arg Gly Ile Asp Lys Ala Val Thr Ala Ala Val
115 120 125
Glu Glu Leu Lys Ala Leu Ser Val Pro Cys Ser Asp Ser Lys Ala Ile
130 135 140
Ala Gln Val Gly Thr Ile Ser Ala Asn Ser Asp Glu Thr Val Gly Lys
145 150 155 160
Leu Ile Ala Glu Ala Met Asp Lys Val Gly Lys Glu Gly Val Ile Thr
165 170 175
Val Glu Asp Gly Thr Gly Leu Gln Asp Glu Leu Asp Val Val Glu Gly
180 185 190
Met Gln Phe Asp Arg Gly Tyr Leu Ser Pro Tyr Phe Ile Asn Lys Pro
195 200 205
Glu Thr Gly Ala Val Glu Leu Glu Ser Pro Phe Ile Leu Leu Ala Asp
210 215 220
Lys Lys Ile Ser Asn Ile Arg Glu Met Leu Pro Val Leu Glu Ala Val
225 230 235 240
Ala Lys Ala Gly Lys Pro Leu Leu Ile Ile Ala Glu Asp Val Glu Gly
245 250 255
Glu Ala Leu Ala Thr Leu Val Val Asn Thr Met Arg Gly Ile Val Lys
260 265 270
Val Ala Ala Val Lys Ala Pro Gly Phe Gly Asp Arg Arg Lys Ala Met
275 280 285
Leu Gln Asp Ile Ala Thr Leu Thr Gly Gly Thr Val Ile Ser Glu Glu
290 295 300
Ile Gly Met Glu Leu Glu Lys Ala Thr Leu Glu Asp Leu Gly Gln Ala
305 310 315 320
Lys Arg Val Val Ile Asn Lys Asp Thr Thr Thr Ile Ile Asp Gly Val
325 330 335
Gly Glu Glu Ala Ala Ile Gln Gly Arg Val Ala Gln Ile Arg Gln Gln
340 345 350
Ile Glu Glu Ala Thr Ser Asp Tyr Asp Arg Glu Lys Leu Gln Glu Arg
355 360 365
Val Ala Lys Leu Ala Gly Gly Val Ala Val Ile Lys Val Gly Ala Ala
370 375 380
Thr Glu Val Glu Met Lys Glu Lys Lys Ala Arg Val Glu Asp Ala Leu
385 390 395 400
His Ala Thr Arg Ala Ala Val Glu Glu Gly Val Val Ala Gly Gly Gly
405 410 415
Val Ala Leu Ile Arg Val Ala Ser Lys Leu Ala Asp Leu Arg Gly Gln
420 425 430
Asn Glu Asp Gln Asn Val Gly Ile Lys Val Ala Leu Arg Ala Met Glu
435 440 445
Ala Pro Leu Arg Gln Ile Val Leu Asn Cys Gly Glu Glu Pro Ser Val
450 455 460
Val Ala Asn Thr Val Lys Gly Gly Asp Gly Asn Tyr Gly Tyr Asn Ala
465 470 475 480
Ala Thr Glu Glu Tyr Gly Asn Met Ile Asp Met Gly Ile Leu Asp Pro
485 490 495
Thr Lys Val Thr Arg Ser Ala Leu Gln Tyr Ala Ala Ser Val Ala Gly
500 505 510
Leu Met Ile Thr Thr Glu Cys Met Val Thr Asp Leu Pro Lys Asn Asp
515 520 525
Ala Ala Asp Leu Gly Ala Ala Gly Gly Met Gly Gly Met Gly Gly Met
530 535 540
Gly Gly Met Met
545
<210> 53
<211> 474
<212> PRT
<213> 福氏志贺氏菌
<400> 53
Met Ser Thr Glu Ile Lys Thr Gln Val Val Val Leu Gly Ala Gly Pro
1 5 10 15
Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr
20 25 30
Val Ile Val Glu Arg Tyr Asn Thr Leu Gly Gly Val Cys Leu Asn Val
35 40 45
Gly Cys Ile Pro Ser Lys Ala Leu Leu His Val Ala Lys Val Ile Glu
50 55 60
Glu Ala Lys Ala Leu Ala Glu His Gly Ile Val Phe Gly Glu Pro Lys
65 70 75 80
Thr Asp Ile Asp Lys Ile Arg Thr Trp Lys Glu Lys Val Ile Asn Gln
85 90 95
Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Gly Arg Lys Val Lys Val
100 105 110
Val Asn Gly Leu Gly Lys Phe Thr Gly Ala Asn Thr Leu Glu Val Glu
115 120 125
Gly Glu Asn Gly Lys Thr Val Ile Asn Phe Asp Asn Ala Ile Ile Ala
130 135 140
Ala Gly Ser Arg Pro Ile Gln Leu Pro Phe Ile Pro His Glu Asp Pro
145 150 155 160
Arg Ile Trp Asp Ser Thr Asp Ala Leu Glu Leu Lys Glu Val Pro Glu
165 170 175
Arg Leu Leu Val Met Gly Gly Gly Ile Ile Gly Leu Glu Met Gly Thr
180 185 190
Val Tyr His Ala Leu Gly Ser Gln Ile Asp Val Val Glu Met Phe Asp
195 200 205
Gln Val Ile Pro Ala Ala Asp Lys Asp Ile Val Lys Val Phe Thr Lys
210 215 220
Arg Ile Ser Lys Lys Phe Asn Leu Met Leu Glu Thr Lys Val Thr Ala
225 230 235 240
Val Glu Ala Lys Glu Asp Gly Ile Tyr Val Thr Met Glu Gly Lys Lys
245 250 255
Ala Pro Ala Glu Pro Gln Arg Tyr Asp Ala Val Leu Val Ala Ile Gly
260 265 270
Arg Val Pro Asn Gly Lys Asn Leu Asp Ala Gly Lys Ala Gly Val Glu
275 280 285
Val Asp Asp Arg Gly Phe Ile Arg Val Asp Lys Gln Leu Arg Thr Asn
290 295 300
Val Pro His Ile Phe Ala Ile Gly Asp Ile Val Gly Gln Pro Met Leu
305 310 315 320
Ala His Lys Gly Val His Glu Gly His Val Ala Ala Glu Val Ile Ala
325 330 335
Gly Lys Lys His Tyr Phe Asp Pro Lys Val Ile Pro Ser Ile Ala Tyr
340 345 350
Thr Glu Pro Glu Val Ala Trp Val Gly Leu Thr Glu Lys Glu Ala Lys
355 360 365
Glu Lys Gly Ile Ser Tyr Glu Thr Ala Thr Phe Pro Trp Ala Ala Ser
370 375 380
Gly Arg Ala Ile Ala Ser Asp Cys Ala Asp Gly Met Thr Lys Leu Ile
385 390 395 400
Phe Asp Lys Glu Ser His Arg Val Ile Gly Gly Ala Ile Val Gly Thr
405 410 415
Asn Gly Gly Glu Leu Leu Gly Glu Ile Gly Leu Ala Ile Glu Met Gly
420 425 430
Cys Asp Ala Glu Asp Ile Ala Leu Thr Ile His Ala His Pro Thr Leu
435 440 445
His Glu Ser Val Gly Leu Ala Ala Glu Val Phe Glu Gly Ser Ile Thr
450 455 460
Asp Leu Pro Asn Pro Lys Ala Lys Lys Lys
465 470
<210> 54
<211> 112
<212> PRT
<213> 福氏志贺氏菌
<400> 54
Met Asn Lys Asn Met Ala Gly Ile Leu Ser Ala Ala Ala Val Leu Thr
1 5 10 15
Met Leu Ala Gly Cys Thr Ala Tyr Asp Arg Thr Lys Asp Gln Phe Val
20 25 30
Gln Pro Val Val Lys Asp Val Lys Lys Gly Met Ser Arg Ala Gln Val
35 40 45
Ala Gln Ile Ala Gly Lys Pro Ser Ser Glu Val Ser Met Ile His Ala
50 55 60
Arg Gly Thr Cys Gln Thr Tyr Ile Leu Gly Gln Arg Asp Gly Lys Ala
65 70 75 80
Glu Thr Tyr Phe Val Ala Leu Asp Asp Thr Gly His Val Ile Asn Ser
85 90 95
Gly Tyr Gln Thr Cys Ala Glu Tyr Asp Thr Asp Pro Gln Ala Ala Lys
100 105 110
<210> 55
<211> 239
<212> PRT
<213> 福氏志贺氏菌
<400> 55
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val
1 5 10 15
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr
20 25 30
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly
35 40 45
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly
50 55 60
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp
65 70 75 80
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu
85 90 95
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr
100 105 110
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala
115 120 125
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala
130 135 140
Leu Gly Val Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe
145 150 155 160
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile
165 170 175
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu
180 185 190
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg
195 200 205
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp
210 215 220
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu
225 230 235
<210> 56
<211> 388
<212> PRT
<213> 福氏志贺氏菌
<400> 56
Met Asn Leu His Glu Tyr Gln Ala Lys Gln Leu Phe Ala Arg Tyr Gly
1 5 10 15
Leu Pro Ala Pro Val Gly Tyr Ala Cys Thr Thr Pro Arg Glu Ala Glu
20 25 30
Glu Ala Ala Ser Lys Ile Gly Ala Gly Pro Trp Val Val Lys Cys Gln
35 40 45
Val His Ala Gly Gly Arg Gly Lys Ala Gly Gly Val Lys Val Val Asn
50 55 60
Ser Lys Glu Asp Ile Arg Ala Phe Ala Glu Asn Trp Leu Gly Lys Arg
65 70 75 80
Leu Val Thr Tyr Gln Thr Asp Ala Asn Gly Gln Pro Val Asn Gln Ile
85 90 95
Leu Val Glu Ala Ala Thr Asp Ile Ala Lys Glu Leu Tyr Leu Gly Ala
100 105 110
Val Val Asp Arg Ser Ser Arg Arg Val Val Phe Met Ala Ser Thr Glu
115 120 125
Gly Gly Val Glu Ile Glu Lys Val Ala Glu Glu Thr Pro His Leu Ile
130 135 140
His Lys Val Ala Leu Asp Pro Leu Thr Gly Pro Met Pro Tyr Gln Gly
145 150 155 160
Arg Glu Leu Ala Phe Lys Leu Gly Leu Glu Gly Lys Leu Val Gln Gln
165 170 175
Phe Thr Lys Ile Phe Met Gly Leu Ala Thr Ile Phe Leu Glu Arg Asp
180 185 190
Leu Ala Leu Ile Glu Ile Asn Pro Leu Val Ile Thr Lys Gln Gly Asp
195 200 205
Leu Ile Cys Leu Asp Gly Lys Leu Gly Ala Asp Gly Asn Ala Leu Phe
210 215 220
Arg Gln Pro Asp Leu Arg Glu Met Arg Asp Gln Ser Gln Glu Asp Pro
225 230 235 240
Arg Glu Ala Gln Ala Ala Gln Trp Glu Leu Asn Tyr Val Ala Leu Asp
245 250 255
Gly Asn Ile Gly Cys Met Val Asn Gly Ala Gly Leu Ala Met Gly Thr
260 265 270
Met Asp Ile Val Lys Leu His Gly Gly Glu Pro Ala Asn Phe Leu Asp
275 280 285
Val Gly Gly Gly Ala Thr Lys Glu Arg Val Thr Glu Ala Phe Lys Ile
290 295 300
Ile Leu Ser Asp Asp Lys Val Lys Ala Val Leu Val Asn Ile Phe Gly
305 310 315 320
Gly Ile Val Arg Cys Asp Leu Ile Ala Asp Gly Ile Ile Gly Ala Val
325 330 335
Ala Glu Val Gly Val Asn Val Pro Val Val Val Arg Leu Glu Gly Asn
340 345 350
Asn Ala Glu Leu Gly Ala Lys Lys Leu Ala Asp Ser Gly Leu Asn Ile
355 360 365
Ile Ala Ala Lys Gly Leu Thr Asp Ala Ala Gln Gln Val Val Ala Ala
370 375 380
Val Glu Gly Lys
385
<210> 57
<211> 169
<212> PRT
<213> 福氏志贺氏菌
<400> 57
Met Gly Leu Phe Asp Lys Leu Lys Ser Leu Val Ser Asp Asp Lys Lys
1 5 10 15
Asp Thr Gly Thr Ile Glu Ile Ile Ala Pro Leu Ser Gly Glu Ile Val
20 25 30
Asn Ile Glu Asp Val Pro Asp Val Val Phe Ala Glu Lys Ile Val Gly
35 40 45
Asp Gly Ile Ala Ile Lys Pro Thr Gly Asn Lys Met Val Ala Pro Val
50 55 60
Asp Gly Thr Ile Gly Lys Ile Phe Glu Thr Asn His Ala Phe Ser Ile
65 70 75 80
Glu Ser Asp Ser Gly Val Glu Leu Phe Val His Phe Gly Ile Asp Thr
85 90 95
Val Glu Leu Lys Gly Glu Gly Phe Lys Arg Ile Ala Glu Glu Gly Gln
100 105 110
Arg Val Lys Val Gly Asp Thr Val Ile Glu Phe Asp Leu Pro Leu Leu
115 120 125
Glu Glu Lys Ala Lys Ser Thr Leu Thr Pro Val Val Ile Ser Asn Met
130 135 140
Asp Glu Ile Lys Glu Leu Ile Lys Leu Ser Gly Ser Val Thr Val Gly
145 150 155 160
Glu Thr Pro Val Ile Arg Ile Lys Lys
165
<210> 58
<211> 638
<212> PRT
<213> 福氏志贺氏菌
<400> 58
Met Gly Lys Ile Ile Gly Ile Asp Leu Gly Thr Thr Asn Ser Cys Val
1 5 10 15
Ala Ile Met Asp Gly Thr Ile Pro Arg Val Leu Glu Asn Ala Glu Gly
20 25 30
Asp Arg Thr Thr Pro Ser Ile Ile Ala Tyr Thr Gln Asp Gly Glu Thr
35 40 45
Leu Val Gly Gln Pro Ala Lys Arg Gln Ala Val Thr Asn Pro Gln Asn
50 55 60
Thr Leu Phe Ala Ile Lys Arg Leu Ile Gly Arg Arg Phe Gln Asp Glu
65 70 75 80
Glu Val Gln Arg Asp Val Ser Ile Met Pro Phe Lys Ile Ile Ala Ala
85 90 95
Asp Asn Gly Asp Ala Trp Val Glu Val Lys Gly Gln Lys Met Ala Pro
100 105 110
Pro Gln Ile Ser Ala Glu Val Leu Lys Lys Met Lys Lys Thr Ala Glu
115 120 125
Asp Tyr Leu Gly Glu Pro Val Thr Glu Ala Val Ile Thr Val Pro Ala
130 135 140
Tyr Phe Asn Asp Ala Gln Arg Gln Ala Thr Lys Asp Ala Gly Arg Ile
145 150 155 160
Ala Gly Leu Glu Val Lys Arg Ile Ile Asn Glu Pro Thr Ala Ala Ala
165 170 175
Leu Ala Tyr Gly Leu Asp Lys Gly Thr Gly Asn Arg Thr Ile Ala Val
180 185 190
Tyr Asp Leu Gly Gly Gly Thr Phe Asp Ile Ser Ile Ile Glu Ile Asp
195 200 205
Glu Val Asp Gly Glu Lys Thr Phe Glu Val Leu Ala Thr Asn Gly Asp
210 215 220
Thr His Leu Gly Gly Glu Asp Phe Asp Ser Arg Leu Ile Asn Tyr Leu
225 230 235 240
Val Glu Glu Phe Lys Lys Asp Gln Gly Ile Asp Leu Arg Asn Asp Pro
245 250 255
Leu Ala Met Gln Arg Leu Lys Glu Ala Ala Glu Lys Ala Lys Ile Glu
260 265 270
Leu Ser Ser Ala Gln Gln Thr Asp Val Asn Leu Pro Tyr Ile Thr Ala
275 280 285
Asp Ala Thr Gly Pro Lys His Met Asn Ile Lys Val Thr Arg Ala Lys
290 295 300
Leu Glu Ser Leu Val Glu Asp Leu Val Asn Arg Ser Ile Glu Pro Leu
305 310 315 320
Lys Val Ala Leu Gln Asp Ala Gly Leu Ser Val Ser Asp Ile Asp Asp
325 330 335
Val Ile Leu Val Gly Gly Gln Thr Arg Met Pro Met Val Gln Lys Lys
340 345 350
Val Ala Glu Phe Phe Gly Lys Glu Pro Arg Lys Asp Val Asn Pro Asp
355 360 365
Glu Ala Val Ala Ile Gly Ala Ala Val Gln Gly Gly Val Leu Thr Gly
370 375 380
Asp Val Lys Asp Val Leu Leu Leu Asp Val Thr Pro Leu Ser Leu Gly
385 390 395 400
Ile Glu Thr Met Gly Gly Val Met Thr Thr Leu Ile Ala Lys Asn Thr
405 410 415
Thr Ile Pro Thr Lys His Ser Gln Val Phe Ser Thr Ala Glu Asp Asn
420 425 430
Gln Ser Ala Val Thr Ile His Val Leu Gln Gly Glu Arg Lys Arg Ala
435 440 445
Ala Asp Asn Lys Ser Leu Gly Gln Phe Asn Leu Asp Gly Ile Asn Pro
450 455 460
Ala Pro Arg Gly Met Pro Gln Ile Glu Val Thr Phe Asp Ile Asp Ala
465 470 475 480
Asp Gly Ile Leu His Val Ser Ala Lys Asp Lys Asn Ser Gly Lys Glu
485 490 495
Gln Lys Ile Thr Ile Lys Ala Ser Ser Gly Leu Asn Glu Asp Glu Ile
500 505 510
Gln Lys Met Val Arg Asp Ala Glu Ala Asn Ala Glu Ala Asp Arg Lys
515 520 525
Phe Glu Glu Leu Val Gln Thr Arg Asn Gln Gly Asp His Leu Leu His
530 535 540
Ser Thr Arg Lys Gln Val Glu Glu Ala Gly Asp Lys Leu Pro Ala Asp
545 550 555 560
Asp Lys Thr Ala Ile Glu Ser Ala Leu Thr Ala Leu Glu Thr Ala Leu
565 570 575
Lys Gly Glu Asp Lys Ala Ala Ile Glu Ala Lys Met Gln Glu Leu Ala
580 585 590
Gln Val Ser Gln Lys Leu Met Glu Ile Ala Gln Gln Gln His Ala Gln
595 600 605
Gln Gln Thr Ala Gly Ala Asp Ala Ser Ala Asn Asn Ala Lys Asp Asp
610 615 620
Asp Val Val Asp Ala Glu Phe Glu Glu Val Lys Asp Lys Lys
625 630 635
<210> 59
<211> 190
<212> PRT
<213> 宋内志贺氏菌
<400> 59
Met Lys Leu Val His Met Ala Ser Cys Leu Ala Val Ala Ile Ala Leu
1 5 10 15
Ala Ala Cys Ala Asp Lys Ser Ala Asp Ile Gln Thr Pro Ala Pro Ala
20 25 30
Ala Asn Thr Ser Ile Ser Ala Thr Gln Gln Pro Ala Ile Gln Gln Pro
35 40 45
Asn Val Ser Gly Thr Val Trp Ile Arg Gln Lys Val Ala Leu Pro Pro
50 55 60
Asp Ala Val Leu Thr Val Thr Leu Ser Asp Ala Ser Leu Ala Asp Ala
65 70 75 80
Pro Ser Lys Val Leu Ala Gln Lys Ala Val Arg Thr Glu Gly Lys Gln
85 90 95
Ser Pro Phe Ser Phe Val Leu Pro Phe Asn Pro Ala Asp Val Gln Pro
100 105 110
Asn Ala Arg Ile Leu Leu Ser Ala Ala Ile Thr Val Asn Asp Lys Leu
115 120 125
Val Phe Ile Thr Asp Thr Val Gln Pro Val Ile Asn Lys Gly Gly Thr
130 135 140
Lys Ala Asp Leu Thr Leu Val Pro Met Gln Gln Thr Ala Val Pro Val
145 150 155 160
Gln Ala Ser Gly Gly Ala Thr Thr Thr Val Pro Ser Thr Ser Pro Thr
165 170 175
Gln Val Asn Pro Ser Ser Ala Val Pro Ala Pro Thr Gln Tyr
180 185 190
<210> 60
<211> 101
<212> PRT
<213> 福氏志贺氏菌
<400> 60
Met Ser Lys Glu His Thr Thr Glu His Leu Arg Ala Glu Leu Lys Ser
1 5 10 15
Leu Ser Asp Thr Leu Glu Glu Val Leu Ser Ser Ser Gly Glu Lys Ser
20 25 30
Lys Glu Glu Leu Ser Lys Ile Arg Ser Lys Ala Glu Gln Ala Leu Lys
35 40 45
Gln Ser Arg Tyr Arg Leu Gly Glu Thr Gly Asp Ala Ile Ala Lys Gln
50 55 60
Thr Arg Val Ala Ala Ala Arg Ala Asp Glu Tyr Val Arg Glu Asn Pro
65 70 75 80
Trp Thr Gly Val Gly Ile Gly Ala Ala Ile Gly Val Val Leu Gly Val
85 90 95
Leu Leu Ser Arg Arg
100
<210> 61
<211> 330
<212> PRT
<213> 痢疾志贺氏菌
<400> 61
Met Arg Asn Ile Ile Lys Leu Ala Leu Val Gly Leu Leu Ser Val Ser
1 5 10 15
Thr Leu Ala Val Ala Ala Glu Ser Ser Pro Glu Ala Leu Arg Ile Gly
20 25 30
Tyr Gln Lys Ser Asn Ile Ser Ser Val Leu Ala Lys Asn Tyr Gln Met
35 40 45
Leu Glu Lys Arg Tyr Pro Gln Thr Lys Ile Ser Trp Gly Lys Phe Pro
50 55 60
Ala Gly Pro Gln Met Leu Glu Ala Leu Asn Val Gly Ser Ile Asp Leu
65 70 75 80
Gly Ser Thr Gly Asp Ile Pro Pro Ile Phe Ser Gln Ala Ala Gly Ala
85 90 95
Asp Leu Leu Tyr Val Ser Val Glu Pro Pro Lys Pro Lys Ala Glu Val
100 105 110
Ile Leu Val Ala Glu Asn Ser Pro Ile Lys Thr Val Ala Asp Leu Lys
115 120 125
Gly His Lys Val Ala Phe Gln Lys Gly Ser Ser Ser His Asn Leu Leu
130 135 140
Leu Arg Ala Leu Arg Gln Ala Gly Leu Lys Phe Thr Asp Ile Gln Pro
145 150 155 160
Thr Tyr Leu Thr Pro Ala Asp Ala Arg Ala Ala Phe Gln Gln Gly Asn
165 170 175
Val Asp Ala Trp Ala Ile Trp Asp Pro Tyr Tyr Ser Ala Ala Leu Leu
180 185 190
Gln Gly Gly Val Arg Val Leu Lys Asp Gly Thr Asp Leu Asn Gln Thr
195 200 205
Gly Ser Phe Tyr Leu Ala Ala Arg Pro Tyr Ala Glu Lys Asn Gly Ala
210 215 220
Phe Ile Gln Gly Val Leu Ala Thr Phe Ser Glu Ala Asp Ala Leu Thr
225 230 235 240
Arg Ser Gln Arg Glu Gln Ser Ile Ala Leu Leu Ala Lys Thr Met Gly
245 250 255
Leu Pro Ala Pro Val Ile Ala Ser Tyr Leu Asp His Arg Pro Pro Thr
260 265 270
Thr Ile Lys Pro Val Asn Ala Glu Val Ala Ala Leu Gln Gln Gln Thr
275 280 285
Ala Asp Leu Phe Tyr Glu Asn Arg Leu Val Pro Lys Lys Val Asp Ile
290 295 300
Asn Ala Ser Gly Ser Pro Leu Asn Trp Lys Glu Asn Asn Tyr Glu Ser
305 310 315 320
Glu Tyr Val Leu Val Phe Thr Asp Pro Trp
325 330
<210> 62
<211> 678
<212> PRT
<213> 宋内志贺氏菌
<400> 62
Met Val Pro Ser Thr Phe Ser Arg Leu Lys Ala Ala Arg Cys Leu Pro
1 5 10 15
Val Val Leu Ala Ala Leu Ile Phe Ala Gly Cys Gly Thr His Thr Pro
20 25 30
Asp Gln Ser Thr Ala Tyr Met Gln Gly Thr Ala Gln Ala Asp Ser Ala
35 40 45
Phe Tyr Leu Gln Gln Met Gln Gln Ser Ser Asp Asp Thr Arg Ile Asn
50 55 60
Trp Gln Leu Leu Ala Ile Arg Ala Leu Val Lys Glu Gly Lys Thr Gly
65 70 75 80
Gln Ala Val Glu Leu Phe Asn Gln Leu Pro Gln Glu Leu Asn Asp Ser
85 90 95
Gln Arg Arg Glu Lys Thr Leu Leu Ala Val Glu Ile Lys Leu Ala Gln
100 105 110
Lys Asp Phe Ala Gly Ala Gln Asn Leu Leu Ala Lys Ile Thr Pro Ala
115 120 125
Asp Leu Glu Gln Asn Gln Gln Ala Arg Tyr Trp Gln Ala Lys Ile Asp
130 135 140
Ala Ser Gln Gly Arg Pro Ser Ile Asp Leu Leu Arg Ala Leu Ile Ala
145 150 155 160
Gln Glu Pro Leu Leu Gly Ala Lys Glu Lys Lys Gln Asn Ile Asp Ala
165 170 175
Thr Trp Gln Ala Leu Ser Ser Met Thr Gln Glu Gln Ala Asn Thr Leu
180 185 190
Val Ile Asn Ala Asp Glu Asn Ile Leu Gln Gly Trp Leu Asp Leu Gln
195 200 205
Arg Val Trp Phe Asp Asn Arg Asn Asp Pro Asp Met Met Lys Ala Gly
210 215 220
Ile Ala Asp Trp Gln Lys Arg Tyr Pro Asn Asn Pro Gly Ala Lys Met
225 230 235 240
Leu Pro Thr Gln Leu Val Asn Val Lys Ala Phe Lys Pro Ala Ser Thr
245 250 255
Asn Lys Ile Ala Leu Leu Leu Pro Leu Asn Gly Gln Ala Ala Val Phe
260 265 270
Gly Arg Thr Ile Gln Gln Gly Phe Glu Ala Ala Lys Asn Ile Gly Thr
275 280 285
Gln Pro Val Ala Ala Gln Val Ala Ala Ala Pro Ala Ala Asp Val Ala
290 295 300
Glu Gln Pro Gln Pro Gln Thr Val Asp Gly Val Ala Ser Pro Ala Gln
305 310 315 320
Ala Ser Val Ser Asp Leu Thr Gly Asp Gln Pro Ala Ala Gln Pro Val
325 330 335
Pro Val Ser Ala Pro Ala Thr Ser Thr Ala Ala Val Ser Ala Pro Ala
340 345 350
Asn Pro Ser Ala Glu Leu Lys Ile Tyr Asp Thr Ser Ser Gln Pro Leu
355 360 365
Ser Gln Ile Leu Ser Gln Val Gln Gln Asp Gly Ala Ser Ile Val Val
370 375 380
Gly Pro Leu Leu Lys Asn Asn Val Glu Glu Leu Leu Lys Ser Asn Thr
385 390 395 400
Pro Leu Asn Val Leu Ala Leu Asn Gln Pro Glu Asn Ile Glu Asn Arg
405 410 415
Val Asn Ile Cys Tyr Phe Ala Leu Ser Pro Glu Asp Glu Ala Arg Asp
420 425 430
Ala Ala Arg His Ile Arg Asp Gln Gly Lys Gln Ala Pro Leu Val Leu
435 440 445
Ile Pro Arg Ser Ser Leu Gly Asp Arg Val Ala Asn Ala Phe Ala Gln
450 455 460
Glu Trp Gln Lys Leu Gly Gly Gly Thr Val Leu Gln Gln Lys Phe Gly
465 470 475 480
Ser Thr Ser Glu Leu Arg Ala Gly Val Asn Gly Gly Ser Gly Ile Ala
485 490 495
Leu Thr Gly Thr Pro Ile Thr Pro Arg Ala Thr Thr Asp Ser Gly Met
500 505 510
Thr Thr Asn Asn Pro Thr Leu Gln Thr Thr Pro Thr Asp Asp Gln Phe
515 520 525
Thr Asn Asn Gly Gly Arg Val Asp Ala Val Tyr Ile Val Ala Thr Pro
530 535 540
Gly Glu Ile Ala Phe Ile Lys Pro Met Ile Ala Met Arg Asn Gly Ser
545 550 555 560
Gln Ser Gly Ala Met Leu Tyr Ala Ser Ser Arg Ser Ala Gln Gly Thr
565 570 575
Ala Gly Pro Asp Phe Arg Leu Glu Met Glu Gly Leu Gln Tyr Ser Glu
580 585 590
Ile Pro Met Leu Ala Gly Gly Asn Leu Pro Leu Met Gln Gln Ala Leu
595 600 605
Ser Ala Val Asn Asn Asp Tyr Ser Leu Ala Arg Met Tyr Ala Met Gly
610 615 620
Val Asp Ala Trp Ser Leu Ala Asn His Phe Ser Gln Met Arg Gln Val
625 630 635 640
Gln Gly Phe Glu Ile Asn Gly Asn Thr Gly Ser Leu Thr Ala Asn Pro
645 650 655
Asp Cys Val Ile Asn Arg Lys Leu Ser Trp Leu Gln Tyr Gln Gln Gly
660 665 670
Gln Val Val Pro Val Ser
675
<210> 63
<211> 392
<212> PRT
<213> 福氏志贺氏菌
<400> 63
Met Gln Leu Arg Lys Leu Leu Leu Pro Gly Leu Leu Ser Val Thr Leu
1 5 10 15
Leu Ser Gly Cys Ser Leu Phe Asn Ser Glu Glu Asp Val Val Lys Met
20 25 30
Ser Pro Leu Pro Thr Val Glu Asn Gln Phe Thr Pro Thr Thr Ala Trp
35 40 45
Ser Thr Ser Val Gly Ser Gly Ile Gly Asn Phe Tyr Ser Asn Leu His
50 55 60
Pro Ala Leu Ala Asp Asn Val Val Tyr Ala Ala Asp Arg Ala Gly Leu
65 70 75 80
Val Lys Ala Leu Asn Ala Asp Asp Gly Lys Glu Ile Trp Ser Val Ser
85 90 95
Leu Ala Glu Lys Asp Gly Trp Phe Ser Lys Glu Pro Ala Leu Leu Ser
100 105 110
Gly Gly Val Thr Val Ser Gly Gly His Val Tyr Ile Gly Ser Glu Lys
115 120 125
Ala Gln Val Tyr Ala Leu Asn Thr Ser Asp Gly Thr Val Ala Trp Gln
130 135 140
Thr Lys Val Ala Gly Glu Ala Leu Ser Arg Pro Val Val Ser Asp Gly
145 150 155 160
Leu Val Leu Ile His Thr Ser Asn Gly Gln Leu Gln Ala Leu Asn Glu
165 170 175
Ala Asp Gly Ala Val Lys Trp Thr Val Asn Leu Asp Met Pro Ser Leu
180 185 190
Ser Leu Arg Gly Glu Ser Ala Pro Ala Thr Ala Phe Gly Ala Ala Val
195 200 205
Val Gly Gly Asp Asn Gly Arg Val Ser Ala Val Leu Met Glu Gln Gly
210 215 220
Gln Met Ile Trp Gln Gln Arg Ile Ser Gln Ala Thr Gly Ser Thr Glu
225 230 235 240
Ile Asp Arg Leu Ser Asp Val Asp Thr Thr Pro Val Val Val Asn Gly
245 250 255
Val Val Phe Ala Leu Ala Tyr Asn Gly Asn Leu Thr Ala Leu Asp Leu
260 265 270
Arg Ser Gly Gln Ile Met Trp Lys Arg Glu Leu Gly Ser Val Asn Asp
275 280 285
Phe Ile Val Asp Gly Asn Arg Ile Tyr Leu Val Asp Gln Asn Asp Arg
290 295 300
Val Met Ala Leu Thr Ile Asp Gly Gly Val Thr Leu Trp Ala Gln Ser
305 310 315 320
Asp Leu Leu His Arg Leu Leu Thr Ser Pro Val Leu Tyr Asn Gly Asn
325 330 335
Leu Val Val Gly Asp Ser Glu Gly Tyr Leu His Trp Ile Asn Val Glu
340 345 350
Asp Gly Arg Phe Val Ala Gln Gln Lys Val Asp Ser Ser Gly Phe Gln
355 360 365
Thr Glu Pro Val Ala Ala Asp Gly Lys Leu Leu Ile Gln Ala Lys Asp
370 375 380
Gly Thr Val Tyr Ser Ile Thr Arg
385 390
<210> 64
<211> 306
<212> PRT
<213> 福氏志贺氏菌
<400> 64
Met Asn Met Lys Leu Lys Thr Leu Phe Ala Ala Ala Phe Ala Val Val
1 5 10 15
Gly Phe Cys Ser Thr Ala Ser Ala Val Thr Tyr Pro Leu Pro Thr Asp
20 25 30
Gly Ser Arg Leu Val Gly Gln Asn Gln Val Ile Thr Ile Pro Glu Gly
35 40 45
Asn Thr Gln Pro Leu Glu Tyr Phe Ala Ala Glu Tyr Gln Met Gly Leu
50 55 60
Ser Asn Met Met Glu Ala Asn Pro Gly Val Asp Thr Phe Leu Pro Lys
65 70 75 80
Gly Gly Thr Val Leu Asn Ile Pro Gln Gln Leu Ile Leu Pro Asp Thr
85 90 95
Val His Glu Gly Ile Val Ile Asn Ser Ala Glu Met Arg Leu Tyr Tyr
100 105 110
Tyr Pro Lys Gly Thr Asn Thr Val Ile Val Leu Pro Ile Gly Ile Gly
115 120 125
Gln Leu Gly Lys Asp Thr Pro Ile Asn Trp Thr Thr Lys Val Glu His
130 135 140
Lys Lys Ala Gly Pro Thr Trp Thr Pro Thr Ala Lys Met His Ala Glu
145 150 155 160
Tyr Arg Ala Ala Gly Glu Pro Leu Pro Ala Val Val Pro Ala Gly Pro
165 170 175
Asp Asn Pro Met Gly Leu Tyr Ala Leu Tyr Ile Gly Arg Leu Tyr Ala
180 185 190
Ile His Gly Thr Asn Ala Asn Phe Gly Ile Gly Leu Arg Val Ser His
195 200 205
Gly Cys Val Arg Leu Arg Asn Glu Asp Ile Lys Phe Leu Phe Glu Lys
210 215 220
Val Pro Val Gly Thr Arg Val Gln Phe Ile Asp Glu Pro Val Lys Ala
225 230 235 240
Thr Thr Glu Pro Asp Gly Ser Arg Tyr Ile Glu Val His Asn Pro Leu
245 250 255
Ser Thr Thr Glu Ala Gln Phe Glu Gly Gln Glu Ile Val Pro Ile Thr
260 265 270
Leu Thr Lys Ser Val Gln Thr Val Thr Gly Gln Pro Asp Val Asp Gln
275 280 285
Val Val Leu Asp Glu Ala Ile Lys Asn Arg Ser Gly Met Pro Val Arg
290 295 300
Leu Asn
305
<210> 65
<211> 298
<212> PRT
<213> 福氏志贺氏菌
<400> 65
Met Asn Val Thr Val Ala Tyr Gln Thr Ser Ala Glu Pro Ala Lys Val
1 5 10 15
Ala Gln Ala Asp Asn Thr Phe Ala Lys Glu Ser Gly Ala Ser Val Asp
20 25 30
Trp Arg Lys Phe Asp Ser Gly Ala Ser Ile Val Arg Ala Leu Ala Ser
35 40 45
Gly Asp Val Gln Ile Gly Asn Leu Gly Ser Ser Pro Leu Ala Val Ala
50 55 60
Ala Ser Gln Gln Val Pro Ile Glu Val Phe Leu Leu Ala Ser Lys Leu
65 70 75 80
Gly Asn Ser Glu Ala Leu Val Val Lys Lys Thr Ile Ser Lys Pro Glu
85 90 95
Asp Leu Ile Gly Lys Arg Ile Ala Val Pro Phe Ile Ser Thr Thr His
100 105 110
Tyr Ser Leu Leu Ala Ala Leu Lys His Trp Gly Ile Lys Pro Gly Leu
115 120 125
Val Glu Ile Val Asn Leu Gln Pro Pro Ala Ile Ile Ala Ala Trp Gln
130 135 140
Arg Gly Asp Ile Asp Gly Ala Tyr Val Trp Ala Pro Ala Val Asn Ala
145 150 155 160
Leu Glu Lys Asp Gly Lys Val Leu Thr Asp Ser Glu Gln Val Gly Gln
165 170 175
Trp Gly Ala Pro Thr Leu Asp Val Trp Val Val Arg Lys Asp Phe Ala
180 185 190
Glu Lys His Pro Glu Val Val Lys Ala Phe Ala Lys Ser Ala Ile Asp
195 200 205
Ala Gln Gln Pro Tyr Ile Ala Asn Pro Asp Ala Trp Leu Lys Gln Pro
210 215 220
Glu Asn Ile Ser Lys Leu Ala Arg Leu Ser Gly Val Pro Glu Gly Asp
225 230 235 240
Val Pro Gly Leu Val Lys Gly Asn Thr Tyr Leu Thr Pro Gln Gln Gln
245 250 255
Thr Ala Glu Leu Thr Gly Pro Val Asn Lys Ala Ile Ile Asp Thr Ala
260 265 270
Gln Phe Leu Lys Glu Gln Gly Lys Val Pro Ala Val Ala Asn Asp Tyr
275 280 285
Ser Gln Tyr Val Thr Ser Arg Phe Val Gln
290 295
<210> 66
<211> 67
<212> PRT
<213> 福氏志贺氏菌
<400> 66
Met Lys Asn Val Phe Lys Ala Leu Thr Val Leu Leu Thr Leu Phe Ser
1 5 10 15
Leu Thr Gly Cys Gly Leu Lys Gly Pro Leu Tyr Phe Pro Pro Ala Asp
20 25 30
Lys Asn Ala Pro Pro Pro Thr Lys Pro Val Glu Thr Gln Thr Gln Ser
35 40 45
Thr Val Pro Asp Lys Asn Asp Arg Ala Thr Gly Asp Gly Pro Ser Gln
50 55 60
Val Asn Tyr
65
<210> 67
<211> 251
<212> PRT
<213> 福氏志贺氏菌
<400> 67
Met Lys Leu Arg Leu Ser Ala Leu Ala Leu Gly Thr Thr Leu Leu Val
1 5 10 15
Gly Cys Ala Ser Ser Gly Thr Asp Gln Gln Gly Arg Ser Asp Pro Leu
20 25 30
Glu Gly Phe Asn Arg Thr Met Tyr Asn Phe Asn Phe Asn Val Leu Asp
35 40 45
Pro Tyr Ile Val Arg Pro Val Ala Val Ala Trp Arg Asp Tyr Val Pro
50 55 60
Gln Pro Ala Arg Asn Gly Leu Ser Asn Phe Thr Gly Asn Leu Glu Glu
65 70 75 80
Pro Ala Val Met Val Asn Tyr Phe Leu Gln Gly Asp Pro Tyr Gln Gly
85 90 95
Met Val His Phe Thr Arg Phe Phe Leu Asn Thr Ile Leu Gly Met Gly
100 105 110
Gly Phe Ile Asp Val Ala Gly Met Ala Asn Pro Lys Leu Gln Arg Thr
115 120 125
Glu Pro His Arg Phe Gly Ser Thr Leu Gly His Tyr Gly Val Gly Tyr
130 135 140
Gly Pro Tyr Val Gln Leu Pro Phe Tyr Gly Ser Phe Thr Leu Arg Asp
145 150 155 160
Asp Gly Gly Asp Met Ala Asp Ala Leu Tyr Pro Val Leu Ser Trp Leu
165 170 175
Thr Trp Pro Met Ser Val Gly Lys Trp Thr Leu Glu Gly Ile Glu Thr
180 185 190
Arg Ala Gln Leu Leu Asp Ser Asp Gly Leu Leu Arg Gln Ser Ser Asp
195 200 205
Pro Tyr Ile Met Val Arg Glu Ala Tyr Phe Gln Arg His Asp Phe Ile
210 215 220
Ala Asn Gly Gly Glu Leu Lys Pro Gln Glu Asn Pro Asn Ala Gln Ala
225 230 235 240
Ile Gln Asp Asp Leu Lys Asp Ile Asp Ser Glu
245 250
<210> 68
<211> 249
<212> PRT
<213> 福氏志贺氏菌
<400> 68
Met Lys Arg Gln Leu Phe Thr Leu Ser Ile Val Gly Val Phe Ser Leu
1 5 10 15
Asn Thr Phe Ala Ser Ile Pro Pro Gly Asn Asp Val Thr Thr Lys Pro
20 25 30
Asp Leu Tyr Tyr Leu Thr Asn Asp Asn Ala Ile Asp Ser Leu Ala Leu
35 40 45
Leu Pro Pro Pro Pro Gln Ile Gly Ser Ile Ala Phe Leu Asn Asp Gln
50 55 60
Ala Met Tyr Glu Lys Gly Arg Leu Leu Arg Asn Thr Glu Arg Gly Lys
65 70 75 80
Leu Ala Ala Glu Asp Ala Asn Leu Ser Ser Gly Gly Val Ala Asn Val
85 90 95
Phe Ser Ala Ala Phe Gly Ser Pro Ile Thr Ala Lys Asp Ser Pro Glu
100 105 110
Leu His Lys Leu Leu Thr Asn Met Ile Glu Asp Ala Gly Asp Leu Ala
115 120 125
Thr Arg Ser Ala Lys Glu Tyr Tyr Met Arg Ile Arg Pro Phe Ala Phe
130 135 140
Tyr Gly Val Ser Thr Cys Asn Thr Lys Glu Gln Asp Thr Leu Ser Arg
145 150 155 160
Asn Gly Ser Tyr Pro Ser Gly His Thr Ser Ile Gly Trp Ala Thr Ala
165 170 175
Leu Val Leu Ser Glu Ile Asn Pro Ala Arg Gln Asp Thr Ile Leu Lys
180 185 190
Arg Gly Tyr Glu Leu Gly Asp Ser Arg Val Ile Cys Gly Tyr His Trp
195 200 205
Gln Ser Asp Val Asp Ala Ala Arg Ile Val Gly Ser Ala Ile Val Ala
210 215 220
Thr Leu His Ser Asn Pro Val Phe Gln Ala Gln Leu Gln Lys Ala Lys
225 230 235 240
Asp Glu Phe Ala Asn Asn Gln Lys Lys
245
<210> 69
<211> 566
<212> PRT
<213> 福氏志贺氏菌
<400> 69
Met Lys Lys Phe Asn Ile Lys Ser Leu Thr Leu Leu Ile Val Leu Leu
1 5 10 15
Pro Leu Ile Val Asn Ala Asn Asn Ile Asp Ser His Leu Leu Glu Gln
20 25 30
Asn Asp Ile Ala Lys Tyr Val Ala Gln Ser Asp Thr Val Gly Ser Phe
35 40 45
Phe Glu Arg Phe Ser Ala Leu Leu Asn Tyr Pro Ile Val Val Ser Lys
50 55 60
Gln Ala Ala Lys Lys Arg Ile Ser Gly Glu Phe Asp Leu Ser Asn Pro
65 70 75 80
Glu Glu Met Leu Glu Lys Leu Thr Leu Leu Val Gly Leu Ile Trp Tyr
85 90 95
Lys Asp Gly Asn Ala Leu Tyr Ile Tyr Asp Ser Gly Glu Leu Ile Ser
100 105 110
Lys Val Ile Leu Leu Glu Asn Ile Ser Leu Asn Tyr Leu Ile Gln Tyr
115 120 125
Leu Lys Asp Ala Asn Leu Tyr Asp His Arg Tyr Pro Ile Arg Gly Asn
130 135 140
Ile Ser Asp Lys Thr Phe Tyr Ile Ser Gly Pro Pro Ala Leu Val Glu
145 150 155 160
Leu Val Ala Asn Thr Ala Thr Leu Leu Asp Lys Gln Val Ser Ser Ile
165 170 175
Gly Thr Asp Lys Val Asn Phe Gly Val Ile Lys Leu Lys Asn Thr Phe
180 185 190
Val Ser Asp Arg Thr Tyr Asn Met Arg Gly Glu Asp Ile Val Ile Pro
195 200 205
Gly Val Ala Thr Val Val Glu Arg Leu Leu Asn Asn Gly Lys Ala Leu
210 215 220
Ser Asn Arg Gln Ala Gln Asn Asp Pro Met Pro Pro Phe Asn Ile Thr
225 230 235 240
Gln Lys Val Ser Glu Asp Ser Asn Asp Phe Ser Phe Ser Ser Val Thr
245 250 255
Asn Ser Ser Ile Leu Glu Asp Val Ser Leu Ile Ala Tyr Pro Glu Thr
260 265 270
Asn Ser Ile Leu Val Lys Gly Asn Asp Gln Gln Ile Gln Ile Ile Arg
275 280 285
Asp Ile Ile Thr Gln Leu Asp Val Ala Lys Arg His Ile Glu Leu Ser
290 295 300
Leu Trp Ile Ile Asp Ile Asp Lys Ser Glu Leu Asn Asn Leu Gly Val
305 310 315 320
Asn Trp Gln Gly Thr Ala Ser Phe Gly Asp Ser Phe Gly Ala Ser Phe
325 330 335
Asn Met Ser Ser Ser Ala Ser Ile Ser Thr Leu Asp Gly Asn Lys Phe
340 345 350
Ile Ala Ser Val Met Ala Leu Asn Gln Lys Lys Lys Ala Asn Val Val
355 360 365
Ser Arg Pro Val Ile Leu Thr Gln Glu Asn Ile Pro Ala Ile Phe Asp
370 375 380
Asn Asn Arg Thr Phe Tyr Val Ser Leu Val Gly Glu Arg Asn Ser Ser
385 390 395 400
Leu Glu His Val Thr Tyr Gly Thr Leu Ile Asn Val Ile Pro Arg Phe
405 410 415
Ser Ser Arg Gly Gln Ile Glu Met Ser Leu Thr Ile Glu Asp Gly Thr
420 425 430
Gly Asn Ser Gln Ser Asn Tyr Asn Tyr Asn Asn Glu Asn Thr Ser Val
435 440 445
Leu Pro Glu Val Gly Arg Thr Lys Ile Ser Thr Ile Ala Arg Val Pro
450 455 460
Gln Gly Lys Ser Leu Leu Ile Gly Gly Tyr Thr His Glu Thr Asn Ser
465 470 475 480
Asn Glu Ile Ile Ser Ile Pro Phe Leu Ser Ser Ile Pro Val Ile Gly
485 490 495
Asn Val Phe Lys Tyr Lys Thr Ser Asn Ile Ser Asn Ile Val Arg Val
500 505 510
Phe Leu Ile Gln Pro Arg Glu Ile Lys Glu Ser Ser Tyr Tyr Asn Thr
515 520 525
Ala Glu Tyr Lys Ser Leu Ile Ser Glu Arg Glu Ile Gln Lys Thr Thr
530 535 540
Gln Ile Ile Pro Ser Glu Thr Thr Leu Leu Glu Asp Glu Lys Ser Leu
545 550 555 560
Val Ser Tyr Leu Asn Tyr
565
<210> 70
<211> 222
<212> PRT
<213> 福氏志贺氏菌
<400> 70
Met Gly Ile Phe Ser Arg Phe Ala Asp Ile Val Asn Ala Asn Ile Asn
1 5 10 15
Ala Leu Leu Glu Lys Ala Glu Asp Pro Gln Lys Leu Val Arg Leu Met
20 25 30
Ile Gln Glu Met Glu Asp Thr Leu Val Glu Val Arg Ser Thr Ser Ala
35 40 45
Arg Ala Leu Ala Glu Lys Lys Gln Leu Thr Arg Arg Ile Glu Gln Ala
50 55 60
Ser Ala Arg Glu Val Glu Trp Gln Glu Lys Ala Glu Leu Ala Leu Leu
65 70 75 80
Lys Glu Arg Glu Asp Leu Ala Arg Ala Ala Leu Ile Glu Lys Gln Lys
85 90 95
Leu Thr Asp Leu Ile Lys Ser Leu Glu His Glu Val Thr Leu Val Asp
100 105 110
Asp Thr Leu Ala Arg Met Lys Lys Glu Ile Gly Glu Leu Glu Asn Lys
115 120 125
Leu Ser Glu Thr Arg Ala Arg Gln Gln Ala Leu Met Leu Arg His Gln
130 135 140
Ala Ala Asn Ser Ser Arg Asp Val Arg Arg Gln Leu Asp Ser Gly Lys
145 150 155 160
Leu Asp Glu Ala Met Ala Arg Phe Glu Ser Phe Glu Arg Arg Ile Asp
165 170 175
Gln Met Glu Ala Glu Ala Glu Ser His Ser Phe Gly Lys Gln Lys Ser
180 185 190
Leu Asp Asp Gln Phe Ala Glu Leu Lys Ala Asp Asp Ala Ile Ser Glu
195 200 205
Gln Leu Ala Gln Leu Lys Ala Lys Met Lys Gln Asp Asn Gln
210 215 220
<210> 71
<211> 248
<212> PRT
<213> 福氏志贺氏菌
<400> 71
Met Thr Lys Leu Lys Leu Leu Ala Leu Gly Val Leu Ile Ala Thr Ser
1 5 10 15
Ala Gly Val Ala His Ala Glu Gly Lys Phe Ser Leu Gly Ala Gly Val
20 25 30
Gly Val Val Glu His Pro Tyr Lys Asp Tyr Asp Thr Asp Val Tyr Pro
35 40 45
Val Pro Val Ile Asn Tyr Glu Gly Asp Asn Phe Trp Phe Arg Gly Leu
50 55 60
Gly Gly Gly Tyr Tyr Leu Trp Asn Asp Ala Thr Asp Lys Leu Ser Ile
65 70 75 80
Thr Ala Tyr Trp Ser Pro Leu Tyr Phe Lys Ala Lys Asp Ser Gly Asp
85 90 95
His Gln Met Arg His Leu Asp Asp Arg Lys Ser Thr Met Met Ala Gly
100 105 110
Leu Ser Tyr Ala His Phe Thr Gln Tyr Gly Tyr Leu Arg Thr Thr Leu
115 120 125
Ala Gly Asp Thr Leu Asp Asn Ser Asn Gly Ile Val Trp Asp Met Ala
130 135 140
Trp Leu Tyr Arg Tyr Thr Asn Gly Gly Leu Thr Val Thr Pro Gly Ile
145 150 155 160
Gly Val Gln Trp Asn Ser Glu Asn Gln Asn Glu Tyr Tyr Tyr Gly Val
165 170 175
Ser Arg Lys Glu Ser Ala Arg Ser Gly Leu Arg Gly Tyr Asn Pro Asn
180 185 190
Asp Ser Trp Ser Pro Tyr Leu Glu Leu Ser Ala Ser Tyr Asn Phe Leu
195 200 205
Gly Asp Trp Ser Val Tyr Gly Thr Ala Arg Tyr Thr Arg Leu Ser Asp
210 215 220
Glu Val Thr Asp Ser Pro Met Val Asp Lys Ser Trp Thr Gly Leu Ile
225 230 235 240
Ser Thr Gly Ile Thr Tyr Lys Phe
245
<210> 72
<211> 285
<212> PRT
<213> 福氏志贺氏菌
<400> 72
Met Phe Thr Asn Asn Gly Ser Thr Leu Gln Thr Asp Ile Thr Thr Phe
1 5 10 15
Gly Val Asn Met Lys Leu Ala His Leu Gly Arg Gln Ala Leu Met Gly
20 25 30
Val Met Ala Val Ala Leu Val Ala Gly Met Ser Val Lys Ser Phe Ala
35 40 45
Asp Glu Gly Leu Leu Asn Lys Val Lys Glu Ser Gly Thr Leu Leu Val
50 55 60
Gly Leu Glu Gly Thr Tyr Pro Pro Phe Ser Phe Gln Gly Asp Asp Gly
65 70 75 80
Lys Leu Thr Gly Phe Glu Val Glu Phe Ala Gln Gln Leu Ala Lys His
85 90 95
Leu Gly Val Glu Ala Ser Leu Lys Pro Thr Lys Trp Asp Gly Met Leu
100 105 110
Ala Ser Leu Asp Ser Lys Arg Ile Asp Val Val Ile Asn Gln Val Thr
115 120 125
Ile Ser Asp Glu Arg Lys Lys Lys Tyr Asp Phe Ser Thr Pro Tyr Thr
130 135 140
Ile Ser Gly Ile Gln Ala Leu Val Lys Lys Gly Asn Glu Gly Thr Ile
145 150 155 160
Lys Thr Ala Ala Asp Leu Lys Gly Lys Lys Val Gly Val Gly Leu Gly
165 170 175
Thr Asn Tyr Glu Glu Trp Leu Arg Gln Asn Val Gln Gly Val Asp Val
180 185 190
Arg Thr Tyr Asp Asp Asp Pro Thr Lys Tyr Gln Asp Leu Arg Val Gly
195 200 205
Arg Ile Asp Ala Ile Leu Val Asp Arg Leu Ala Ala Leu Asp Leu Val
210 215 220
Lys Lys Thr Asn Asp Thr Leu Ala Val Thr Gly Glu Ala Phe Ser Arg
225 230 235 240
Gln Glu Ser Gly Val Ala Leu Arg Lys Gly Asn Glu Asp Leu Leu Lys
245 250 255
Ala Val Asn Asp Ala Ile Ala Glu Met Gln Lys Asp Gly Thr Leu Gln
260 265 270
Ala Leu Ser Glu Lys Trp Phe Gly Ala Asp Val Thr Lys
275 280 285
<210> 73
<211> 245
<212> PRT
<213> 福氏志贺氏菌
<400> 73
Met Thr Arg Met Lys Tyr Leu Val Ala Ala Ala Thr Leu Ser Leu Phe
1 5 10 15
Leu Ala Gly Cys Ser Gly Ser Lys Glu Glu Val Pro Asp Asn Pro Pro
20 25 30
Asn Glu Ile Tyr Ala Thr Ala Gln Gln Lys Leu Gln Asp Gly Asn Trp
35 40 45
Arg Gln Ala Ile Thr Gln Leu Glu Ala Leu Asp Asn Arg Tyr Pro Phe
50 55 60
Gly Pro Tyr Ser Gln Gln Val Gln Leu Asp Leu Ile Tyr Ala Tyr Tyr
65 70 75 80
Lys Asn Ala Asp Leu Pro Leu Ala Gln Ala Ala Ile Asp Arg Phe Ile
85 90 95
Arg Leu Asn Pro Thr His Pro Asn Ile Asp Tyr Val Met Tyr Met Arg
100 105 110
Gly Leu Thr Asn Met Ala Leu Asp Asp Ser Ala Leu Gln Gly Phe Phe
115 120 125
Gly Val Asp Arg Ser Asp Arg Asp Pro Gln His Ala Arg Ala Ala Phe
130 135 140
Ser Asp Phe Ser Lys Leu Val Arg Gly Tyr Pro Asn Ser Gln Tyr Thr
145 150 155 160
Thr Asp Ala Thr Lys Arg Leu Val Phe Leu Lys Asp Arg Leu Ala Lys
165 170 175
Tyr Glu Tyr Ser Val Ala Glu Tyr Tyr Thr Glu Arg Gly Ala Trp Val
180 185 190
Ala Val Val Asn Arg Ile Glu Gly Met Leu Arg Asp Tyr Pro Asp Thr
195 200 205
Gln Ala Thr Arg Asp Ala Leu Pro Leu Met Glu Asn Ala Tyr Arg Gln
210 215 220
Met Gln Met Asn Ala Gln Ala Glu Lys Val Ala Lys Ile Ile Ala Ala
225 230 235 240
Asn Ser Ser Asn Thr
245
<210> 74
<211> 1285
<212> PRT
<213> 福氏志贺氏菌
<400> 74
Met Asn Lys Ile Tyr Ser Leu Lys Tyr Ser His Ile Thr Gly Gly Leu
1 5 10 15
Val Ala Val Ser Glu Leu Thr Arg Lys Val Ser Val Gly Thr Ser Arg
20 25 30
Lys Lys Val Ile Leu Gly Ile Ile Leu Ser Ser Ile Tyr Gly Ser Tyr
35 40 45
Gly Glu Thr Ala Phe Ala Ala Met Leu Asp Ile Asn Asn Ile Trp Thr
50 55 60
Arg Asp Tyr Leu Asp Leu Ala Gln Asn Arg Gly Glu Phe Arg Pro Gly
65 70 75 80
Ala Thr Asn Val Gln Leu Met Met Lys Asp Gly Lys Ile Phe His Phe
85 90 95
Pro Glu Leu Pro Val Pro Asp Phe Ser Ala Val Ser Asn Lys Gly Ala
100 105 110
Thr Thr Ser Ile Gly Gly Ala Tyr Ser Val Thr Ala Thr His Asn Gly
115 120 125
Thr Gln His His Ala Ile Thr Thr Gln Ser Trp Asp Gln Thr Ala Tyr
130 135 140
Lys Ala Ser Asn Arg Val Ser Ser Gly Asp Phe Ser Val His Arg Leu
145 150 155 160
Asn Lys Phe Val Val Glu Thr Thr Gly Val Thr Glu Ser Ala Asp Phe
165 170 175
Ser Leu Ser Pro Glu Asp Ala Met Lys Arg Tyr Gly Val Asn Tyr Asn
180 185 190
Gly Lys Glu Gln Ile Ile Gly Phe Arg Ala Gly Ala Gly Thr Thr Ser
195 200 205
Thr Ile Leu Asn Gly Lys Gln Tyr Leu Phe Gly Gln Asn Tyr Asn Pro
210 215 220
Asp Leu Leu Ser Ala Ser Leu Phe Asn Leu Asp Trp Lys Asn Lys Ser
225 230 235 240
Tyr Ile Tyr Thr Asn Arg Thr Pro Phe Lys Asn Ser Pro Ile Phe Gly
245 250 255
Asp Ser Gly Ser Gly Ser Tyr Leu Tyr Asp Lys Glu Gln Gln Lys Trp
260 265 270
Val Phe His Gly Val Thr Ser Thr Val Gly Phe Ile Ser Ser Thr Asn
275 280 285
Ile Ala Trp Thr Asn Tyr Ser Leu Phe Asn Asn Ile Leu Val Asn Asn
290 295 300
Leu Lys Lys Asn Phe Thr Asn Thr Met Gln Leu Asp Gly Lys Lys Gln
305 310 315 320
Glu Leu Ser Ser Ile Ile Lys Asp Lys Asp Leu Ser Val Ser Gly Gly
325 330 335
Gly Val Leu Thr Leu Lys Gln Asp Thr Asp Leu Gly Ile Gly Gly Leu
340 345 350
Ile Phe Asp Lys Asn Gln Thr Tyr Lys Val Tyr Gly Lys Asp Lys Ser
355 360 365
Tyr Lys Gly Ala Gly Ile Asp Ile Asp Asn Asn Thr Thr Val Glu Trp
370 375 380
Asn Val Lys Gly Val Ala Gly Asp Asn Leu His Lys Ile Gly Ser Gly
385 390 395 400
Thr Leu Asp Val Lys Ile Ala Gln Gly Asn Asn Leu Lys Ile Gly Asn
405 410 415
Gly Thr Val Ile Leu Ser Ala Glu Lys Ala Phe Asn Lys Ile Tyr Met
420 425 430
Ala Gly Gly Lys Gly Thr Val Lys Ile Asn Ala Lys Asp Ala Leu Ser
435 440 445
Glu Ser Gly Asn Gly Glu Ile Tyr Phe Thr Arg Asn Gly Gly Thr Leu
450 455 460
Asp Leu Asn Gly Tyr Asp Gln Ser Phe Gln Lys Ile Ala Ala Thr Asp
465 470 475 480
Ala Gly Thr Thr Val Thr Asn Ser Asn Val Lys Gln Ser Thr Leu Ser
485 490 495
Leu Thr Asn Thr Asp Ala Tyr Met Tyr His Gly Asn Val Ser Gly Asn
500 505 510
Ile Ser Ile Asn His Ile Ile Asn Thr Thr Gln Gln His Asn Asn Asn
515 520 525
Ala Asn Leu Ile Phe Asp Gly Ser Val Asp Ile Lys Asn Asp Ile Ser
530 535 540
Val Arg Asn Ala Gln Leu Thr Leu Gln Gly His Ala Thr Glu His Ala
545 550 555 560
Ile Phe Lys Glu Gly Asn Asn Asn Cys Pro Ile Pro Phe Leu Cys Gln
565 570 575
Lys Asp Tyr Ser Ala Ala Ile Lys Asp Gln Glu Ser Thr Val Asn Lys
580 585 590
Arg Tyr Asn Thr Glu Tyr Lys Ser Asn Asn Gln Ile Ala Ser Phe Ser
595 600 605
Gln Pro Asp Trp Glu Ser Arg Lys Phe Asn Phe Arg Lys Leu Asn Leu
610 615 620
Glu Asn Ala Thr Leu Ser Ile Gly Arg Asp Ala Asn Val Lys Gly His
625 630 635 640
Ile Glu Ala Lys Asn Ser Gln Ile Val Leu Gly Asn Lys Thr Ala Tyr
645 650 655
Ile Asp Met Phe Ser Gly Arg Asn Ile Thr Gly Glu Gly Phe Gly Phe
660 665 670
Arg Gln Gln Leu Arg Ser Gly Asp Ser Ala Gly Glu Ser Ser Phe Asn
675 680 685
Gly Ser Leu Ser Ala Gln Asn Ser Lys Ile Thr Val Gly Asp Lys Ser
690 695 700
Thr Val Thr Met Thr Gly Ala Leu Ser Leu Ile Asn Thr Asp Leu Ile
705 710 715 720
Ile Asn Lys Gly Ala Thr Val Thr Ala Gln Gly Lys Met Tyr Val Asp
725 730 735
Lys Ala Ile Glu Leu Ala Gly Thr Leu Thr Leu Thr Gly Thr Pro Thr
740 745 750
Glu Asn Asn Lys Tyr Ser Pro Ala Ile Tyr Met Ser Asp Gly Tyr Asn
755 760 765
Met Thr Glu Asp Gly Ala Thr Leu Lys Ala Gln Asn Tyr Ala Trp Val
770 775 780
Asn Gly Asn Ile Lys Ser Asp Lys Lys Ala Ser Ile Leu Phe Gly Val
785 790 795 800
Asp Gln Tyr Lys Glu Asp Asn Leu Asp Lys Thr Thr His Thr Pro Leu
805 810 815
Ala Thr Gly Leu Leu Gly Gly Phe Asp Thr Ser Tyr Thr Gly Gly Ile
820 825 830
Asp Ala Pro Ala Ala Ser Ala Ser Met Tyr Asn Thr Leu Trp Arg Val
835 840 845
Asn Gly Gln Ser Ala Leu Gln Ser Leu Lys Thr Arg Asp Ser Leu Leu
850 855 860
Leu Phe Ser Asn Ile Glu Asn Ser Gly Phe His Thr Val Thr Val Asn
865 870 875 880
Thr Leu Asp Ala Thr Asn Thr Ala Val Ile Met Arg Ala Asp Leu Ser
885 890 895
Gln Ser Val Asn Gln Ser Asp Lys Leu Ile Val Lys Asn Gln Leu Thr
900 905 910
Gly Ser Asn Asn Ser Leu Ser Val Asp Ile Gln Lys Val Gly Asn Asn
915 920 925
Asn Ser Gly Leu Asn Val Asp Leu Ile Thr Ala Pro Lys Gly Ser Asn
930 935 940
Lys Glu Ile Phe Lys Ala Ser Thr Gln Ala Ile Gly Phe Ser Asn Ile
945 950 955 960
Ser Pro Val Ile Ser Thr Lys Glu Asp Gln Glu His Thr Thr Trp Thr
965 970 975
Leu Thr Gly Tyr Lys Val Ala Glu Asn Thr Ala Ser Ser Gly Ala Ala
980 985 990
Lys Ser Tyr Met Ser Gly Asn Tyr Lys Ala Phe Leu Thr Glu Val Asn
995 1000 1005
Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Thr Asn Gly Glu Ala
1010 1015 1020
Gly Ala Trp Ala Arg Ile Met Ser Gly Ala Gly Ser Ala Ser Gly Gly
1025 1030 1035 1040
Tyr Ser Asp Asn Tyr Thr His Val Gln Ile Gly Val Asp Lys Lys His
1045 1050 1055
Glu Leu Asp Gly Leu Asp Leu Phe Thr Gly Leu Thr Met Thr Tyr Thr
1060 1065 1070
Asp Ser His Ala Ser Ser Asn Ala Phe Ser Gly Lys Thr Lys Ser Val
1075 1080 1085
Gly Ala Gly Leu Tyr Ala Ser Ala Ile Phe Asp Ser Gly Ala Tyr Ile
1090 1095 1100
Asp Leu Ile Ser Lys Tyr Val His His Asp Asn Glu Tyr Ser Ala Thr
1105 1110 1115 1120
Phe Ala Gly Leu Gly Thr Lys Asp Tyr Ser Ser His Ser Leu Tyr Val
1125 1130 1135
Gly Ala Glu Ala Gly Tyr Arg Tyr His Val Thr Glu Asp Ser Trp Ile
1140 1145 1150
Glu Pro Gln Ala Glu Leu Val Tyr Gly Ala Val Ser Gly Lys Arg Phe
1155 1160 1165
Asp Trp Gln Asp Arg Gly Met Ser Val Thr Met Lys Asp Lys Asp Phe
1170 1175 1180
Asn Pro Leu Ile Gly Arg Thr Gly Val Asp Val Gly Lys Ser Phe Ser
1185 1190 1195 1200
Gly Lys Asp Trp Lys Val Thr Ala Arg Ala Gly Leu Gly Tyr Gln Phe
1205 1210 1215
Asp Leu Phe Ala Asn Gly Glu Thr Val Leu Arg Asp Ala Ser Gly Glu
1220 1225 1230
Lys Arg Ile Lys Gly Glu Lys Asp Gly Arg Ile Leu Met Asn Val Gly
1235 1240 1245
Leu Asn Ala Glu Ile Arg Asp Asn Leu Arg Phe Gly Leu Glu Phe Glu
1250 1255 1260
Lys Ser Ala Phe Gly Lys Tyr Asn Val Asp Asn Ala Ile Asn Ala Asn
1265 1270 1275 1280
Phe Arg Tyr Ser Phe
1285
<210> 75
<211> 513
<212> PRT
<213> 福氏志贺氏菌
<400> 75
Met Gln Leu Asn Ser Thr Glu Ile Ser Glu Leu Ile Lys Gln Arg Ile
1 5 10 15
Ala Gln Phe Asn Val Val Ser Glu Ala His Asn Glu Gly Thr Ile Val
20 25 30
Ser Val Ser Asp Gly Val Ile Arg Ile His Gly Leu Ala Asp Cys Met
35 40 45
Gln Gly Glu Met Ile Ser Leu Pro Gly Asn Arg Tyr Ala Ile Ala Leu
50 55 60
Asn Leu Glu Arg Asp Ser Val Gly Ala Val Val Met Gly Pro Tyr Ala
65 70 75 80
Asp Leu Ala Glu Gly Met Lys Val Lys Cys Thr Gly Arg Ile Leu Glu
85 90 95
Val Pro Val Gly Arg Gly Leu Leu Gly Arg Val Val Asn Thr Leu Gly
100 105 110
Ala Pro Ile Asp Gly Lys Gly Pro Leu Asp His Asp Gly Phe Ser Ala
115 120 125
Val Glu Ala Ile Ala Pro Gly Val Ile Glu Arg Gln Ser Val Asp Gln
130 135 140
Pro Val Gln Thr Gly Tyr Lys Ala Val Asp Ser Met Ile Pro Ile Gly
145 150 155 160
Arg Gly Gln Arg Glu Leu Ile Ile Gly Asp Arg Gln Thr Gly Lys Thr
165 170 175
Ala Leu Ala Ile Asp Ala Ile Ile Asn Gln Arg Asp Ser Gly Ile Lys
180 185 190
Cys Ile Tyr Val Ala Ile Gly Gln Lys Ala Ser Thr Ile Ser Asn Val
195 200 205
Val Arg Lys Leu Glu Glu His Gly Ala Leu Ala Asn Thr Ile Val Val
210 215 220
Val Ala Thr Ala Ser Glu Ser Ala Ala Leu Gln Tyr Leu Ala Pro Tyr
225 230 235 240
Ala Gly Cys Ala Met Gly Glu Tyr Phe Arg Asp Arg Gly Glu Asp Ala
245 250 255
Leu Ile Ile Tyr Asp Asp Leu Ser Lys Gln Ala Val Ala Tyr Arg Gln
260 265 270
Ile Ser Leu Leu Leu Arg Arg Pro Pro Gly Arg Glu Ala Phe Pro Gly
275 280 285
Asp Val Phe Tyr Leu His Ser Arg Leu Leu Glu Arg Ala Ala Arg Val
290 295 300
Asn Ala Glu Tyr Val Glu Ala Phe Thr Lys Gly Glu Val Lys Gly Lys
305 310 315 320
Thr Gly Ser Leu Thr Ala Leu Pro Ile Ile Glu Thr Gln Ala Gly Asp
325 330 335
Val Ser Ala Phe Val Pro Thr Asn Val Ile Ser Ile Thr Asp Gly Gln
340 345 350
Ile Phe Leu Glu Thr Asn Leu Phe Asn Ala Gly Ile Arg Pro Ala Val
355 360 365
Asn Pro Gly Ile Ser Val Ser Arg Val Gly Gly Ala Ala Gln Thr Lys
370 375 380
Ile Met Lys Lys Leu Ser Gly Gly Ile Arg Thr Ala Leu Ala Gln Tyr
385 390 395 400
Arg Glu Leu Ala Ala Phe Ser Gln Phe Ala Ser Asp Leu Asp Asp Ala
405 410 415
Thr Arg Lys Gln Leu Asp His Gly Gln Lys Val Thr Glu Leu Leu Lys
420 425 430
Gln Lys Gln Tyr Ala Pro Met Ser Val Ala Gln Gln Ser Leu Val Leu
435 440 445
Phe Ala Ala Glu Arg Gly Tyr Leu Ala Asp Val Glu Leu Ser Lys Ile
450 455 460
Gly Ser Phe Glu Ala Ala Leu Leu Ala Tyr Val Asp Arg Asp His Ala
465 470 475 480
Pro Leu Met Gln Glu Ile Asn Gln Thr Gly Gly Tyr Asn Asp Glu Ile
485 490 495
Glu Gly Lys Leu Lys Gly Ile Leu Asp Ser Phe Lys Ala Thr Gln Ser
500 505 510
Trp
<210> 76
<211> 469
<212> PRT
<213> 福氏志贺氏菌
<400> 76
Met Ser Ala Glu His Val Leu Thr Met Leu Asn Glu His Glu Val Lys
1 5 10 15
Phe Val Asp Leu Arg Phe Thr Asp Thr Lys Gly Lys Glu Gln His Val
20 25 30
Thr Ile Pro Ala His Gln Val Asn Ala Glu Phe Phe Glu Glu Gly Lys
35 40 45
Met Phe Asp Gly Ser Ser Ile Gly Gly Trp Lys Gly Ile Asn Glu Ser
50 55 60
Asp Met Val Leu Met Pro Asp Ala Ser Thr Ala Val Ile Asp Pro Phe
65 70 75 80
Phe Ala Asp Ser Thr Leu Ile Ile Arg Cys Asp Ile Leu Glu Pro Gly
85 90 95
Thr Leu Gln Gly Tyr Asp Arg Asp Pro Arg Ser Ile Ala Lys Arg Ala
100 105 110
Glu Asp Tyr Leu Arg Ser Thr Gly Ile Ala Asp Thr Val Leu Phe Gly
115 120 125
Pro Glu Pro Glu Phe Phe Leu Phe Asp Asp Ile Arg Phe Gly Ser Ser
130 135 140
Ile Ser Gly Ser His Val Ala Ile Asp Asp Ile Glu Gly Ala Trp Asn
145 150 155 160
Ser Ser Thr Gln Tyr Glu Gly Gly Asn Lys Gly His Arg Pro Ala Val
165 170 175
Lys Gly Gly Tyr Phe Pro Val Pro Pro Val Asp Ser Ala Gln Asp Ile
180 185 190
Arg Ser Glu Met Cys Leu Val Met Glu Gln Met Gly Leu Val Val Glu
195 200 205
Ala His His His Glu Val Ala Thr Ala Gly Gln Asn Glu Val Ala Thr
210 215 220
Arg Phe Asn Thr Met Thr Lys Lys Ala Asp Glu Ile Gln Ile Tyr Lys
225 230 235 240
Tyr Val Val His Asn Val Ala His Arg Phe Gly Lys Thr Ala Thr Phe
245 250 255
Met Pro Lys Pro Met Phe Gly Asp Asn Gly Ser Gly Met His Cys His
260 265 270
Met Ser Leu Ser Lys Asn Gly Val Asn Leu Phe Ala Gly Asp Lys Tyr
275 280 285
Ala Gly Leu Ser Glu Gln Ala Leu Tyr Tyr Ile Gly Gly Val Ile Lys
290 295 300
His Ala Lys Ala Ile Asn Ala Leu Ala Asn Pro Thr Thr Asn Ser Tyr
305 310 315 320
Lys Arg Leu Val Pro Gly Tyr Glu Ala Pro Val Met Leu Ala Tyr Ser
325 330 335
Ala Arg Asn Arg Ser Ala Ser Ile Arg Ile Pro Val Val Ser Ser Pro
340 345 350
Lys Ala Arg Arg Ile Glu Val Arg Phe Pro Asp Pro Ala Ala Asn Pro
355 360 365
Tyr Leu Cys Phe Ala Ala Leu Leu Met Ala Gly Leu Asp Gly Ile Lys
370 375 380
Asn Lys Ile His Pro Gly Glu Ala Met Asp Lys Asn Leu Tyr Asp Leu
385 390 395 400
Pro Pro Glu Glu Ala Lys Glu Ile Pro Gln Val Ala Gly Ser Leu Glu
405 410 415
Glu Ala Leu Asn Glu Leu Asp Leu Asp Arg Glu Phe Leu Lys Ala Gly
420 425 430
Gly Val Phe Thr Asp Glu Ala Ile Asp Ala Tyr Ile Ala Leu Arg Arg
435 440 445
Glu Glu Asp Asp Arg Val Arg Met Thr Pro His Pro Val Glu Phe Glu
450 455 460
Leu Tyr Tyr Ser Val
465
<210> 77
<211> 626
<212> PRT
<213> 福氏志贺氏菌
<400> 77
Met Ala Ile Glu Ile Lys Val Pro Asp Ile Gly Ala Asp Glu Val Glu
1 5 10 15
Ile Thr Glu Ile Leu Val Lys Val Gly Asp Lys Val Glu Ala Glu Gln
20 25 30
Ser Leu Ile Thr Val Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ser
35 40 45
Pro Gln Ala Gly Ile Val Lys Glu Ile Lys Val Ser Val Gly Asp Lys
50 55 60
Thr Gln Thr Gly Ala Leu Ile Met Ile Phe Asp Ser Ala Asp Gly Ala
65 70 75 80
Ala Asp Ala Ala Pro Ala Gln Ala Glu Glu Lys Lys Glu Ala Ala Pro
85 90 95
Ala Ala Ala Pro Ala Ala Ala Ala Ala Lys Asp Val Asn Val Pro Asp
100 105 110
Ile Gly Ser Asp Glu Val Glu Val Thr Glu Ile Leu Val Lys Val Gly
115 120 125
Asp Lys Val Glu Ala Glu Gln Ser Leu Ile Thr Val Glu Gly Asp Lys
130 135 140
Ala Ser Met Glu Val Pro Ala Pro Phe Ala Gly Thr Val Lys Glu Ile
145 150 155 160
Lys Val Asn Val Gly Asp Lys Val Ser Thr Gly Ser Leu Ile Met Val
165 170 175
Phe Glu Val Ala Gly Glu Ala Gly Ala Ala Ala Pro Ala Ala Lys Gln
180 185 190
Glu Ala Thr Pro Ala Ala Thr Pro Ala Pro Ala Ala Gly Val Lys Glu
195 200 205
Val Asn Val Pro Asp Ile Gly Gly Asp Glu Val Glu Val Thr Glu Val
210 215 220
Met Val Lys Val Gly Asp Lys Val Ala Ala Glu Gln Ser Leu Ile Thr
225 230 235 240
Val Glu Gly Asp Lys Ala Ser Met Glu Val Pro Ala Pro Phe Ala Gly
245 250 255
Val Val Lys Glu Leu Lys Val Asn Val Gly Asp Lys Val Lys Thr Gly
260 265 270
Ser Leu Ile Met Ile Phe Glu Val Glu Gly Ala Ala Pro Ala Ala Ala
275 280 285
Pro Ala Lys Gln Glu Ala Ala Ala Pro Ala Pro Ala Ala Lys Ala Glu
290 295 300
Ala Pro Ala Ala Lys Ala Glu Gly Lys Ser Glu Phe Ala Glu Asn Asp
305 310 315 320
Ala Tyr Val His Ala Thr Pro Leu Ile Arg Arg Leu Ala Arg Glu Phe
325 330 335
Gly Val Asn Leu Ala Lys Val Lys Gly Thr Gly Arg Lys Gly Arg Ile
340 345 350
Leu Arg Glu Asp Val Gln Ala Tyr Val Lys Glu Ala Ile Lys Arg Ala
355 360 365
Glu Ala Ala Pro Ala Ala Thr Gly Gly Gly Ile Pro Gly Met Leu Pro
370 375 380
Trp Pro Lys Val Asp Phe Ser Lys Phe Gly Glu Ile Glu Glu Val Glu
385 390 395 400
Leu Gly Arg Ile Gln Lys Ile Ser Gly Ala Asn Leu Ser Arg Asn Trp
405 410 415
Val Met Ile Pro His Val Thr His Phe Asp Lys Thr Asp Ile Thr Glu
420 425 430
Leu Glu Ala Phe Arg Lys Gln Gln Asn Glu Glu Ala Ala Lys Arg Lys
435 440 445
Leu Asp Val Lys Ile Thr Pro Val Val Phe Ile Met Lys Ala Val Ala
450 455 460
Ala Ala Leu Glu Gln Met Pro Arg Phe Asn Ser Ser Leu Ser Glu Asp
465 470 475 480
Gly Gln Arg Leu Thr Leu Lys Lys Tyr Ile Asn Ile Gly Val Ala Val
485 490 495
Asp Thr Pro Asn Gly Leu Val Val Pro Val Phe Lys Asp Val Asn Lys
500 505 510
Lys Gly Ile Ile Glu Leu Ser Arg Glu Leu Met Thr Ile Ser Lys Lys
515 520 525
Ala Arg Asp Gly Lys Leu Thr Ala Gly Glu Met Gln Gly Gly Cys Phe
530 535 540
Thr Ile Ser Ser Ile Gly Gly Leu Gly Thr Thr His Phe Ala Pro Ile
545 550 555 560
Val Asn Ala Pro Glu Val Ala Ile Leu Gly Val Ser Lys Ser Ala Met
565 570 575
Glu Pro Val Trp Asn Gly Lys Glu Phe Val Pro Arg Leu Met Leu Pro
580 585 590
Ile Ser Leu Ser Phe Asp His Arg Val Ile Asp Gly Ala Asp Gly Ala
595 600 605
Arg Phe Ile Thr Ile Ile Asn Asn Thr Leu Ser Asp Ile Arg Arg Leu
610 615 620
Val Met
625
<210> 78
<211> 289
<212> PRT
<213> 福氏志贺氏菌
<400> 78
Met Ser Ile Leu Ile Asp Lys Asn Thr Lys Val Ile Cys Gln Gly Phe
1 5 10 15
Thr Gly Ser Gln Gly Thr Phe His Ser Glu Gln Ala Ile Ala Tyr Gly
20 25 30
Thr Lys Met Val Gly Gly Val Thr Pro Gly Lys Gly Gly Thr Thr His
35 40 45
Leu Gly Leu Pro Val Phe Asn Thr Val Arg Glu Ala Val Ala Ala Thr
50 55 60
Gly Ala Ser Ala Ser Val Ile Tyr Val Pro Ala Pro Phe Cys Lys Asp
65 70 75 80
Ser Ile Leu Glu Ala Ile Asp Ala Gly Ile Lys Leu Ile Ile Thr Ile
85 90 95
Thr Glu Gly Ile Pro Thr Leu Asp Met Leu Thr Val Lys Val Lys Leu
100 105 110
Asp Glu Ala Gly Val Arg Met Ile Gly Pro Asn Cys Pro Gly Val Ile
115 120 125
Thr Pro Gly Glu Cys Lys Ile Gly Ile Gln Pro Gly His Ile His Lys
130 135 140
Pro Gly Lys Val Gly Ile Val Ser Arg Ser Gly Thr Leu Thr Tyr Glu
145 150 155 160
Ala Val Lys Gln Thr Thr Asp Tyr Gly Phe Gly Gln Ser Thr Cys Val
165 170 175
Gly Ile Gly Gly Asp Pro Ile Pro Gly Ser Asn Phe Ile Asp Ile Leu
180 185 190
Glu Met Phe Glu Lys Asp Pro Gln Thr Glu Ala Ile Val Met Ile Gly
195 200 205
Glu Ile Gly Gly Ser Ala Glu Glu Glu Ala Ala Ala Tyr Ile Lys Glu
210 215 220
His Val Thr Lys Pro Val Val Gly Tyr Ile Ala Gly Val Thr Ala Pro
225 230 235 240
Lys Gly Lys Arg Met Gly His Ala Gly Ala Ile Ile Ala Gly Gly Lys
245 250 255
Gly Thr Ala Asp Glu Lys Phe Ala Ala Leu Glu Ala Ala Gly Val Lys
260 265 270
Thr Val Arg Ser Leu Ala Asp Ile Gly Glu Ala Leu Lys Thr Val Leu
275 280 285
Lys
<210> 79
<211> 405
<212> PRT
<213> 福氏志贺氏菌
<400> 79
Met Ser Ser Val Asp Ile Leu Val Pro Asp Leu Pro Glu Ser Val Ala
1 5 10 15
Asp Ala Thr Val Ala Thr Trp His Lys Lys Pro Gly Asp Ala Val Val
20 25 30
Arg Asp Glu Val Leu Val Glu Ile Glu Thr Asp Lys Val Val Leu Glu
35 40 45
Val Pro Ala Ser Ala Asp Gly Ile Leu Asp Ala Val Leu Glu Asp Glu
50 55 60
Gly Thr Thr Val Thr Ser Arg Gln Ile Leu Gly Arg Leu Arg Glu Gly
65 70 75 80
Asn Ser Ala Gly Lys Glu Thr Ser Ala Lys Ser Glu Glu Lys Ala Ser
85 90 95
Thr Pro Ala Gln Arg Gln Gln Ala Ser Leu Glu Glu Gln Asn Asn Asp
100 105 110
Ala Leu Ser Pro Ala Ile Arg Arg Leu Leu Ala Glu His Asn Leu Asp
115 120 125
Ala Ser Ala Ile Lys Gly Thr Gly Val Gly Gly Arg Leu Thr Arg Glu
130 135 140
Asp Val Glu Lys His Leu Ala Lys Ala Pro Ala Lys Glu Ser Ala Pro
145 150 155 160
Ala Ala Ala Ala Pro Ala Ala Gln Pro Ala Leu Ala Ala Arg Ser Glu
165 170 175
Lys Arg Val Pro Met Thr Arg Leu Arg Lys Arg Val Ala Glu Arg Leu
180 185 190
Leu Glu Ala Lys Asn Ser Thr Ala Met Leu Thr Thr Phe Asn Glu Val
195 200 205
Asn Met Lys Pro Ile Met Asp Leu Arg Lys Gln Tyr Gly Glu Ala Phe
210 215 220
Glu Lys Arg His Gly Ile Arg Leu Gly Phe Met Ser Phe Tyr Val Lys
225 230 235 240
Ala Val Val Glu Ala Leu Lys Arg Tyr Pro Glu Val Asn Ala Ser Ile
245 250 255
Asp Gly Asp Asp Val Val Tyr His Asn Tyr Phe Asp Val Ser Met Ala
260 265 270
Val Ser Thr Pro Arg Gly Leu Val Thr Pro Val Leu Arg Asp Val Asp
275 280 285
Thr Leu Gly Met Ala Asp Ile Glu Lys Lys Ile Lys Glu Leu Ala Val
290 295 300
Lys Gly Arg Asp Gly Lys Leu Thr Val Glu Asp Leu Thr Gly Gly Asn
305 310 315 320
Phe Thr Ile Thr Asn Gly Gly Val Phe Gly Ser Leu Met Ser Thr Pro
325 330 335
Ile Ile Asn Pro Pro Gln Ser Ala Ile Leu Gly Met His Ala Ile Lys
340 345 350
Asp Arg Pro Met Ala Val Asn Gly Gln Val Glu Ile Leu Pro Met Met
355 360 365
Tyr Leu Ala Leu Ser Tyr Asp His Arg Leu Ile Asp Gly Arg Glu Ser
370 375 380
Val Gly Phe Leu Val Thr Ile Lys Glu Leu Leu Glu Glu Pro Thr Arg
385 390 395 400
Leu Leu Leu Asp Val
405
<210> 80
<211> 427
<212> PRT
<213> 福氏志贺氏菌
<400> 80
Met Ala Asp Thr Lys Ala Lys Leu Asn Leu Asn Gly Asp Thr Ala Val
1 5 10 15
Glu Leu Asp Val Leu Lys Gly Thr Leu Gly Gln Asp Val Ile Asp Ile
20 25 30
Arg Thr Leu Gly Ser Lys Gly Val Phe Thr Phe Asp Pro Gly Phe Thr
35 40 45
Ser Thr Ala Ser Cys Glu Ser Lys Ile Thr Phe Ile Asp Gly Asp Glu
50 55 60
Gly Ile Leu Leu His Arg Gly Phe Pro Ile Asp Gln Leu Ala Thr Asp
65 70 75 80
Ser Asn Tyr Leu Glu Val Cys Tyr Ile Leu Leu Asn Gly Glu Lys Pro
85 90 95
Thr Gln Glu Gln Tyr Asp Glu Phe Lys Thr Thr Val Thr Arg His Thr
100 105 110
Met Ile His Glu Gln Ile Thr Arg Leu Phe His Ala Phe Arg Arg Asp
115 120 125
Ser His Pro Met Ala Val Met Cys Gly Ile Thr Gly Ala Leu Ala Ala
130 135 140
Phe Tyr His Asp Ser Leu Asp Val Asn Asn Pro Arg His Arg Glu Ile
145 150 155 160
Ala Ala Phe Arg Leu Leu Ser Lys Met Pro Thr Met Ala Ala Met Cys
165 170 175
Tyr Lys Tyr Ser Ile Gly Gln Pro Phe Val Tyr Pro Arg Asn Asp Leu
180 185 190
Ser Tyr Ala Gly Asn Phe Leu Asn Met Met Phe Ser Thr Pro Cys Glu
195 200 205
Pro Tyr Glu Val Asn Pro Ile Leu Glu Arg Ala Met Asp Arg Ile Leu
210 215 220
Ile Leu His Ala Asp His Glu Gln Asn Ala Ser Thr Ser Thr Val Arg
225 230 235 240
Thr Ala Gly Ser Ser Gly Ala Asn Pro Phe Ala Cys Ile Ala Ala Gly
245 250 255
Ile Ala Ser Leu Trp Gly Pro Ala His Gly Gly Ala Asn Glu Ala Ala
260 265 270
Leu Lys Met Leu Glu Glu Ile Ser Ser Val Lys His Ile Pro Glu Phe
275 280 285
Val Arg Arg Ala Lys Asp Lys Asn Asp Ser Phe Arg Leu Met Gly Phe
290 295 300
Gly His Arg Val Tyr Lys Asn Tyr Asp Pro Arg Ala Thr Val Met Arg
305 310 315 320
Glu Thr Cys His Glu Val Leu Lys Glu Leu Gly Thr Lys Asp Asp Leu
325 330 335
Leu Glu Val Ala Met Glu Leu Glu Asn Ile Ala Leu Asn Asp Pro Tyr
340 345 350
Phe Ile Glu Lys Lys Leu Tyr Pro Asn Val Asp Phe Tyr Ser Gly Ile
355 360 365
Ile Leu Lys Ala Met Gly Ile Pro Ser Ser Met Phe Thr Val Ile Phe
370 375 380
Ala Met Ala Arg Thr Val Gly Trp Ile Ala His Trp Ser Glu Met His
385 390 395 400
Ser Tyr Gly Met Lys Ile Ala Arg Pro Arg Gln Leu Tyr Thr Gly Tyr
405 410 415
Glu Lys Arg Asp Phe Lys Ser Asp Ile Lys Arg
420 425
<210> 81
<211> 403
<212> PRT
<213> 福氏志贺氏菌
<400> 81
Met Asn Thr Ile Phe Ser Ala Arg Ile Met Lys Arg Leu Ala Leu Thr
1 5 10 15
Thr Ala Leu Cys Thr Ala Phe Ile Ser Ala Ala His Ala Asp Asp Leu
20 25 30
Asn Ile Lys Thr Met Ile Pro Gly Val Pro Gln Ile Asp Ala Glu Ser
35 40 45
Tyr Ile Leu Ile Asp Tyr Asn Ser Gly Lys Val Leu Ala Glu Gln Asn
50 55 60
Ala Asp Val Arg Arg Asp Pro Ala Ser Leu Thr Lys Met Met Thr Ser
65 70 75 80
Tyr Val Ile Gly Gln Ala Met Lys Ala Gly Lys Phe Lys Glu Thr Asp
85 90 95
Leu Val Thr Ile Gly Asn Asp Ala Trp Ala Thr Gly Asn Pro Val Phe
100 105 110
Lys Gly Ser Ser Leu Met Phe Leu Lys Pro Gly Met Gln Val Pro Val
115 120 125
Ser Gln Leu Ile Arg Gly Ile Asn Leu Gln Ser Gly Asn Asp Ala Cys
130 135 140
Val Ala Met Ala Asp Phe Ala Ala Gly Ser Gln Asp Ala Phe Val Gly
145 150 155 160
Leu Met Asn Ser Tyr Val Asn Ala Leu Gly Leu Lys Asn Thr His Phe
165 170 175
Gln Thr Val His Gly Leu Asp Ala Asp Gly Gln Tyr Ser Ser Ala Arg
180 185 190
Asp Met Ala Leu Ile Gly Gln Ala Leu Ile Arg Asp Val Pro Asn Glu
195 200 205
Tyr Ser Ile Tyr Lys Glu Lys Glu Phe Thr Phe Asn Gly Ile Arg Gln
210 215 220
Leu Asn Arg Asn Gly Leu Leu Trp Asp Asn Ser Leu Asn Val Asp Gly
225 230 235 240
Ile Lys Thr Gly His Thr Asp Lys Ala Gly Tyr Asn Leu Val Ala Ser
245 250 255
Ala Thr Glu Gly Gln Met Arg Leu Ile Ser Ala Val Met Gly Gly Arg
260 265 270
Thr Phe Lys Gly Arg Glu Ala Glu Ser Lys Lys Leu Leu Thr Trp Gly
275 280 285
Phe Arg Phe Phe Glu Thr Val Asn Pro Leu Lys Val Gly Lys Glu Phe
290 295 300
Ala Ser Glu Pro Val Trp Phe Ser Asp Ser Asp Arg Ala Ser Leu Gly
305 310 315 320
Val Asp Lys Asp Val Tyr Leu Thr Ile Pro Arg Gly Arg Met Lys Asp
325 330 335
Leu Lys Ala Ser Tyr Val Leu Asn Ser Ser Glu Leu His Ala Pro Leu
340 345 350
Gln Lys Asn Gln Val Val Gly Thr Ile Asn Phe Gln Leu Asp Gly Lys
355 360 365
Thr Ile Glu Gln Arg Pro Leu Val Val Leu Gln Glu Ile Pro Glu Gly
370 375 380
Asn Phe Phe Gly Lys Ile Ile Asp Tyr Ile Lys Leu Met Phe His His
385 390 395 400
Trp Phe Gly
<210> 82
<211> 248
<212> PRT
<213> 福氏志贺氏菌
<400> 82
Met Lys Ser Val Leu Lys Val Ser Leu Ala Ala Leu Thr Leu Ala Phe
1 5 10 15
Ala Val Ser Ser His Ala Ala Asp Lys Lys Leu Val Val Ala Thr Asp
20 25 30
Thr Ala Phe Val Pro Phe Glu Phe Lys Gln Gly Asp Lys Tyr Val Gly
35 40 45
Phe Asp Val Asp Leu Trp Ala Ala Ile Ala Lys Glu Leu Lys Leu Asp
50 55 60
Tyr Glu Leu Lys Pro Met Asp Phe Ser Gly Ile Ile Pro Ala Leu Gln
65 70 75 80
Thr Lys Asn Val Asp Leu Ala Leu Ala Gly Ile Thr Ile Thr Asp Glu
85 90 95
Arg Lys Lys Ala Ile Asp Phe Ser Asp Gly Tyr Tyr Lys Ser Gly Leu
100 105 110
Leu Val Met Val Lys Ala Asn Asn Asn Asp Val Lys Ser Val Lys Asp
115 120 125
Leu Asp Gly Lys Val Val Ala Val Lys Ser Gly Thr Gly Ser Val Asp
130 135 140
Tyr Ala Lys Ala Asn Ile Lys Thr Lys Asp Leu Arg Gln Phe Pro Asn
145 150 155 160
Ile Asp Asn Ala Tyr Met Glu Leu Gly Thr Asn Arg Ala Asp Ala Val
165 170 175
Leu His Asp Thr Pro Asn Ile Leu Tyr Phe Ile Lys Thr Ala Gly Asn
180 185 190
Gly Gln Phe Lys Ala Val Gly Asp Ser Leu Glu Ala Gln Gln Tyr Gly
195 200 205
Ile Ala Phe Pro Lys Gly Ser Asp Glu Leu Arg Asp Lys Val Asn Gly
210 215 220
Ala Leu Lys Thr Leu Arg Glu Asn Gly Thr Tyr Asn Glu Ile Tyr Lys
225 230 235 240
Lys Trp Phe Ser Thr Glu Pro Lys
245
<210> 83
<211> 413
<212> PRT
<213> 福氏志贺氏菌
<400> 83
Met Asn Lys Thr Leu Ile Ala Ala Thr Val Ala Gly Ile Val Leu Leu
1 5 10 15
Ala Ser Asn Ala Gln Ala Gln Thr Val Pro Glu Gly Tyr Gln Leu Gln
20 25 30
Gln Val Leu Met Met Ser Arg His Asn Leu Arg Ala Pro Leu Ala Asn
35 40 45
Asn Gly Ser Val Leu Glu Gln Ser Thr Pro Asn Lys Trp Pro Glu Trp
50 55 60
Asp Val Pro Gly Gly Gln Leu Thr Thr Lys Gly Gly Val Leu Glu Val
65 70 75 80
Tyr Met Gly His Tyr Met Arg Glu Trp Leu Ala Glu Gln Gly Ile Val
85 90 95
Lys Ser Gly Glu Cys Pro Pro Pro Asp Thr Val Tyr Ala Tyr Ala Asn
100 105 110
Ser Leu Gln Arg Thr Val Ala Thr Ala Gln Phe Phe Ile Thr Gly Ala
115 120 125
Phe Pro Gly Cys Asp Ile Pro Val His His Gln Glu Lys Met Gly Thr
130 135 140
Met Asp Pro Thr Phe Asn Pro Met Ile Thr Asp Asp Ser Ala Ala Phe
145 150 155 160
Ser Glu Gln Ala Val Ala Ala Met Glu Lys Glu Leu Ser Lys Leu Gln
165 170 175
Leu Thr Asp Ser Tyr Gln Leu Leu Glu Lys Ile Val Asn Tyr Lys Asp
180 185 190
Ser Pro Ala Cys Lys Glu Lys Gln Gln Cys Ser Leu Val Asp Gly Lys
195 200 205
Asn Thr Phe Ser Ala Lys Tyr Gln Gln Glu Pro Gly Val Ser Gly Pro
210 215 220
Leu Lys Val Gly Asn Ser Leu Val Asp Ala Phe Thr Leu Gln Tyr Tyr
225 230 235 240
Glu Gly Phe Pro Met Asp Gln Val Ala Trp Gly Glu Ile Lys Ser Asp
245 250 255
Gln Gln Trp Lys Val Leu Ser Lys Leu Lys Asn Gly Tyr Gln Asp Ser
260 265 270
Leu Phe Thr Ser Pro Glu Val Ala Arg Asn Val Ala Lys Pro Leu Val
275 280 285
Ser Tyr Ile Asp Lys Ala Leu Val Thr Asp Arg Ala Ser Ala Pro Lys
290 295 300
Ile Thr Val Leu Val Gly His Asp Ser Asn Ile Ala Ser Leu Leu Thr
305 310 315 320
Ala Leu Asp Phe Lys Pro Tyr Gln Leu His Asp Gln Asn Glu Arg Thr
325 330 335
Pro Ile Gly Gly Lys Ile Val Phe Gln Arg Trp His Asp Ser Lys Ala
340 345 350
Asn Arg Asp Leu Met Lys Ile Glu Tyr Val Tyr Gln Ser Ala Glu Gln
355 360 365
Leu Arg Asn Ala Asp Ala Leu Thr Leu Gln Ala Pro Ala Gln Arg Val
370 375 380
Thr Leu Glu Leu Ser Gly Cys Pro Ile Asp Ala Asn Gly Phe Cys Pro
385 390 395 400
Met Asp Lys Phe Asp Ser Val Leu Asn Glu Ala Val Lys
405 410
<210> 84
<211> 891
<212> PRT
<213> 福氏志贺氏菌
<400> 84
Met Ala Val Thr Asn Val Ala Glu Leu Asn Ala Leu Val Glu Arg Val
1 5 10 15
Lys Lys Ala Gln Arg Glu Tyr Ala Ser Phe Thr Gln Glu Gln Val Asp
20 25 30
Lys Ile Phe Arg Ala Ala Ala Leu Ala Ala Ala Asp Ala Arg Ile Pro
35 40 45
Leu Ala Lys Met Ala Val Ala Glu Ser Gly Met Gly Ile Val Glu Asp
50 55 60
Lys Val Ile Lys Asn His Phe Ala Ser Glu Tyr Ile Tyr Asn Ala Tyr
65 70 75 80
Lys Asp Glu Lys Thr Cys Gly Val Leu Ser Glu Asp Asp Thr Phe Gly
85 90 95
Thr Ile Thr Ile Ala Glu Pro Ile Gly Ile Ile Cys Gly Ile Val Pro
100 105 110
Thr Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys Ser Leu Ile Ser Leu
115 120 125
Lys Thr Arg Asn Ala Ile Ile Phe Ser Pro His Pro Arg Ala Lys Asp
130 135 140
Ala Thr Asn Lys Ala Ala Asp Ile Val Leu Gln Ala Ala Ile Ala Ala
145 150 155 160
Gly Ala Pro Lys Asp Leu Ile Gly Trp Ile Asp Gln Pro Ser Val Glu
165 170 175
Leu Ser Asn Ala Leu Met His His Pro Asp Ile Asn Leu Ile Leu Ala
180 185 190
Thr Gly Gly Pro Gly Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro
195 200 205
Ala Ile Gly Val Gly Ala Gly Asn Thr Pro Val Val Ile Asp Glu Thr
210 215 220
Ala Asp Ile Lys Arg Ala Val Ala Ser Val Leu Met Ser Lys Thr Phe
225 230 235 240
Asp Asn Gly Val Ile Cys Ala Ser Glu Gln Ser Val Val Val Val Asp
245 250 255
Ser Val Tyr Asp Ala Val Arg Glu Arg Phe Ala Thr His Gly Gly Tyr
260 265 270
Leu Leu Gln Gly Lys Glu Leu Lys Ala Val Gln Asp Val Ile Leu Lys
275 280 285
Asn Gly Ala Leu Asn Ala Ala Ile Val Gly Gln Pro Ala Tyr Lys Ile
290 295 300
Ala Glu Leu Ala Gly Phe Ser Val Pro Glu Asn Thr Lys Ile Leu Ile
305 310 315 320
Gly Glu Val Thr Val Val Asp Glu Ser Glu Pro Phe Ala His Glu Lys
325 330 335
Leu Ser Pro Thr Leu Ala Met Tyr Arg Ala Lys Asp Phe Glu Asp Ala
340 345 350
Val Glu Lys Ala Glu Lys Leu Val Ala Met Gly Gly Ile Gly His Thr
355 360 365
Ser Cys Leu Tyr Thr Asp Gln Asp Asn Gln Pro Ala Arg Val Ser Tyr
370 375 380
Phe Gly Gln Lys Met Lys Thr Ala Arg Ile Leu Ile Asn Thr Pro Ala
385 390 395 400
Ser Gln Gly Gly Ile Gly Asp Leu Tyr Asn Phe Lys Leu Ala Pro Ser
405 410 415
Leu Thr Leu Gly Cys Gly Ser Trp Gly Gly Asn Ser Ile Ser Glu Asn
420 425 430
Val Gly Pro Lys His Leu Ile Asn Lys Lys Thr Val Ala Lys Arg Ala
435 440 445
Glu Asn Met Leu Trp His Lys Leu Pro Lys Ser Ile Tyr Phe Arg Arg
450 455 460
Gly Ser Leu Pro Ile Ala Leu Asp Glu Val Ile Thr Asp Gly His Lys
465 470 475 480
Arg Ala Leu Ile Val Thr Asp Arg Phe Leu Phe Asn Asn Gly Tyr Ala
485 490 495
Asp Gln Ile Thr Ser Val Leu Lys Ala Ala Gly Val Glu Thr Glu Val
500 505 510
Phe Phe Glu Val Glu Ala Asp Pro Thr Leu Ser Ile Val Arg Lys Gly
515 520 525
Ala Glu Leu Ala Asn Ser Phe Lys Pro Asp Val Ile Ile Ala Leu Gly
530 535 540
Gly Gly Ser Pro Met Asp Ala Ala Lys Ile Met Trp Val Met Tyr Glu
545 550 555 560
His Pro Glu Thr His Phe Glu Glu Leu Ala Leu Arg Phe Met Asp Ile
565 570 575
Arg Lys Arg Ile Tyr Lys Phe Pro Lys Met Gly Val Lys Ala Lys Met
580 585 590
Ile Ala Val Thr Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Pro Phe
595 600 605
Ala Val Val Thr Asp Asp Ala Thr Gly Gln Lys Tyr Pro Leu Ala Asp
610 615 620
Tyr Ala Leu Thr Pro Asp Met Ala Ile Val Asp Ala Asn Leu Val Met
625 630 635 640
Asp Met Pro Lys Ser Leu Cys Ala Phe Gly Gly Leu Asp Ala Val Thr
645 650 655
His Ala Met Glu Ala Tyr Val Ser Val Leu Ala Ser Glu Phe Ser Asp
660 665 670
Gly Gln Ala Leu Gln Ala Leu Lys Leu Leu Lys Glu Tyr Leu Pro Ala
675 680 685
Ser Tyr His Glu Gly Ser Lys Asn Pro Val Ala Arg Glu Arg Val His
690 695 700
Ser Ala Ala Thr Ile Ala Gly Ile Ala Phe Ala Asn Ala Phe Leu Gly
705 710 715 720
Val Cys His Ser Met Ala His Lys Leu Gly Ser Gln Phe His Ile Pro
725 730 735
His Gly Leu Ala Asn Ala Leu Leu Ile Cys Asn Val Ile Arg Tyr Asn
740 745 750
Ala Asn Asp Asn Pro Thr Lys Gln Thr Ala Phe Ser Gln Tyr Asp Arg
755 760 765
Pro Gln Ala Arg Arg Arg Tyr Ala Glu Ile Ala Asp His Leu Gly Leu
770 775 780
Ser Ala Pro Gly Asp Arg Thr Ala Ala Lys Ile Glu Lys Leu Leu Ala
785 790 795 800
Trp Leu Glu Thr Leu Lys Ala Glu Leu Gly Ile Pro Lys Ser Ile Arg
805 810 815
Glu Ala Gly Val Gln Glu Ala Asp Phe Leu Ala Asn Val Asp Lys Leu
820 825 830
Ser Glu Asp Ala Phe Asp Asp Gln Cys Thr Gly Ala Asn Pro Arg Tyr
835 840 845
Pro Leu Ile Ser Glu Leu Lys Gln Ile Leu Leu Asp Thr Tyr Tyr Gly
850 855 860
Arg Asp Tyr Val Glu Asp Glu Thr Ala Ala Lys Lys Glu Ala Ala Pro
865 870 875 880
Ala Lys Ala Glu Lys Lys Ala Lys Lys Ser Ala
885 890
<210> 85
<211> 551
<212> PRT
<213> 福氏志贺氏菌
<400> 85
Met Asp Arg Arg Arg Phe Ile Lys Gly Ser Met Ala Met Ala Ala Val
1 5 10 15
Cys Gly Thr Ser Gly Ile Ala Ser Leu Phe Ser Gln Ala Ala Phe Ala
20 25 30
Ala Asp Ser Asp Ile Ala Asp Gly Gln Thr Gln Arg Phe Asp Phe Ser
35 40 45
Ile Leu Gln Ser Met Ala His Asp Leu Ala Gln Thr Ala Trp Arg Gly
50 55 60
Ala Pro Arg Pro Leu Pro Asp Thr Leu Ala Thr Met Thr Pro Gln Ala
65 70 75 80
Tyr Asn Ser Ile Gln Tyr Asp Ala Glu Lys Ser Leu Trp His Asn Val
85 90 95
Glu Asn Arg Gln Leu Asp Ala Gln Phe Phe His Met Gly Met Gly Phe
100 105 110
Arg Arg Arg Val Arg Met Phe Ser Val Asp Pro Ala Thr His Leu Ala
115 120 125
Arg Glu Ile His Phe Arg Pro Glu Leu Phe Lys Tyr Asn Asp Ala Gly
130 135 140
Val Asp Thr Lys Gln Leu Glu Gly Gln Ser Asp Leu Gly Phe Ala Gly
145 150 155 160
Phe Arg Val Phe Lys Ala Pro Glu Leu Ala Arg Arg Asp Val Val Ser
165 170 175
Phe Leu Gly Ala Ser Tyr Phe Arg Ala Val Asp Asp Thr Tyr Gln Tyr
180 185 190
Gly Leu Ser Ala Arg Gly Leu Ala Ile Asp Thr Tyr Thr Asp Ser Lys
195 200 205
Glu Glu Phe Pro Asp Phe Thr Ala Phe Trp Phe Asp Thr Val Lys Pro
210 215 220
Gly Ala Thr Thr Phe Thr Val Tyr Ala Leu Leu Asp Ser Ala Ser Ile
225 230 235 240
Thr Gly Ala Tyr Lys Phe Thr Ile His Cys Glu Lys Ser Gln Val Ile
245 250 255
Met Asp Val Glu Asn His Leu Tyr Ala Arg Lys Asp Ile Lys Gln Leu
260 265 270
Gly Ile Ser Pro Met Thr Ser Met Phe Ser Cys Gly Thr Asn Glu Arg
275 280 285
Arg Met Cys Asp Thr Ile His Pro Gln Ile His Asp Ser Asp Arg Leu
290 295 300
Ser Met Trp Arg Gly Asn Gly Glu Trp Ile Cys Arg Pro Leu Asn Asn
305 310 315 320
Pro Gln Lys Leu Gln Phe Asn Ala Tyr Thr Asp Asn Asn Pro Lys Gly
325 330 335
Phe Gly Leu Leu Gln Leu Asp Arg Asp Phe Ser His Tyr Gln Asp Ile
340 345 350
Met Gly Trp Tyr Asn Lys Arg Pro Ser Leu Trp Val Glu Pro Arg Asn
355 360 365
Lys Trp Gly Lys Gly Thr Ile Gly Leu Met Glu Ile Pro Thr Thr Gly
370 375 380
Glu Thr Leu Asp Asn Ile Val Cys Phe Trp Gln Pro Glu Lys Ala Val
385 390 395 400
Lys Ala Gly Asp Glu Phe Ala Phe Gln Tyr Arg Leu Tyr Trp Ser Ala
405 410 415
Gln Pro Pro Val His Cys Pro Leu Ala Arg Val Met Ala Thr Arg Thr
420 425 430
Gly Met Gly Gly Phe Pro Glu Gly Trp Ala Pro Gly Glu His Tyr Pro
435 440 445
Glu Lys Trp Ala Arg Arg Phe Ala Val Asp Phe Val Gly Gly Asp Leu
450 455 460
Lys Ala Ala Ala Pro Lys Gly Ile Glu Pro Val Ile Thr Leu Ser Ser
465 470 475 480
Gly Glu Ala Lys Gln Ile Glu Ile Leu Tyr Ile Glu Pro Ile Asp Gly
485 490 495
Tyr Arg Ile Gln Phe Asp Trp Tyr Pro Thr Ser Asp Ser Thr Asp Pro
500 505 510
Val Asp Met Arg Met Tyr Leu Arg Cys Gln Gly Asp Ala Ile Ser Glu
515 520 525
Thr Trp Leu Tyr Gln Tyr Phe Pro Pro Ala Pro Asp Lys Arg Gln Tyr
530 535 540
Val Asp Asp Arg Val Met Ser
545 550
<210> 86
<211> 331
<212> PRT
<213> 福氏志贺氏菌
<400> 86
Met Thr Ile Lys Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Ile
1 5 10 15
Val Phe Arg Ala Ala Gln Lys Arg Ser Asp Ile Glu Ile Val Ala Ile
20 25 30
Asn Asp Leu Leu Asp Ala Asp Tyr Met Ala Tyr Met Leu Lys Tyr Asp
35 40 45
Ser Thr His Gly Arg Phe Asp Gly Thr Val Glu Val Lys Asp Gly His
50 55 60
Leu Ile Val Asn Gly Lys Lys Ile Arg Val Thr Ala Glu Arg Asp Pro
65 70 75 80
Ala Asn Leu Lys Trp Asp Glu Val Gly Val Asp Val Val Ala Glu Ala
85 90 95
Thr Gly Leu Phe Leu Thr Asp Glu Thr Ala Arg Lys His Ile Thr Ala
100 105 110
Gly Ala Lys Lys Val Val Met Thr Gly Pro Ser Lys Asp Asn Thr Pro
115 120 125
Met Phe Val Lys Gly Ala Asn Phe Asp Lys Tyr Ala Gly Gln Asp Ile
130 135 140
Val Ser Asn Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro Leu Ala Lys
145 150 155 160
Val Ile Asn Asp Asn Phe Gly Ile Ile Glu Gly Leu Met Thr Thr Val
165 170 175
His Ala Thr Thr Ala Thr Gln Lys Thr Val Asp Gly Pro Ser His Lys
180 185 190
Asp Trp Arg Gly Gly Arg Gly Ala Ser Gln Asn Ile Ile Pro Ser Ser
195 200 205
Thr Gly Ala Ala Lys Ala Val Gly Lys Val Leu Pro Glu Leu Asn Gly
210 215 220
Lys Leu Thr Gly Met Ala Phe Arg Val Pro Thr Pro Asn Val Ser Val
225 230 235 240
Val Asp Leu Thr Val Arg Leu Glu Lys Ala Ala Thr Tyr Glu Gln Ile
245 250 255
Lys Ala Ala Val Lys Ala Ala Ala Glu Gly Glu Met Lys Gly Val Leu
260 265 270
Gly Tyr Thr Glu Asp Asp Val Val Ser Thr Asp Phe Asn Gly Glu Val
275 280 285
Cys Thr Ser Val Phe Asp Ala Lys Ala Gly Ile Ala Leu Asn Asp Asn
290 295 300
Phe Val Lys Leu Val Ser Trp Tyr Asp Asn Glu Thr Gly Tyr Ser Asn
305 310 315 320
Lys Val Leu Asp Leu Ile Ala His Ile Ser Lys
325 330
<210> 87
<211> 404
<212> PRT
<213> 福氏志贺氏菌
<400> 87
Met Lys Ile Val Lys Ala Glu Val Phe Val Thr Cys Pro Gly Arg Asn
1 5 10 15
Phe Val Thr Leu Lys Ile Thr Thr Glu Asp Gly Ile Thr Gly Leu Gly
20 25 30
Asp Ala Thr Leu Asn Gly Arg Glu Leu Ser Val Ala Ser Tyr Leu Gln
35 40 45
Asp His Leu Cys Pro Arg Leu Ile Gly Arg Asp Ala His Arg Ile Glu
50 55 60
Asp Ile Trp Gln Phe Phe Tyr Lys Gly Ala Tyr Trp Arg Arg Gly Pro
65 70 75 80
Val Thr Met Ser Ala Ile Ser Ala Val Asp Met Ala Leu Trp Asp Ile
85 90 95
Lys Ala Lys Ala Ala Asn Met Pro Leu Tyr Gln Leu Leu Gly Gly Ala
100 105 110
Ser Arg Glu Gly Val Met Val Tyr Cys His Thr Thr Gly His Ser Ile
115 120 125
Asp Glu Ala Leu Asp Asp Tyr Ala Arg His Gln Glu Leu Gly Phe Lys
130 135 140
Ala Ile Arg Val Gln Cys Gly Ile Pro Gly Met Lys Thr Thr Tyr Gly
145 150 155 160
Met Ser Lys Gly Lys Gly Leu Ala Tyr Glu Pro Ala Thr Lys Gly Gln
165 170 175
Trp Pro Glu Glu Gln Leu Trp Ser Thr Glu Lys Tyr Leu Asp Phe Met
180 185 190
Pro Lys Leu Phe Asp Ala Val Arg Asn Lys Phe Gly Phe Asn Glu His
195 200 205
Leu Leu His Asp Met His His Arg Leu Thr Pro Ile Glu Ala Ala Arg
210 215 220
Phe Gly Lys Ser Ile Glu Asp Tyr Arg Met Phe Trp Met Glu Asp Pro
225 230 235 240
Thr Pro Ala Glu Asn Gln Glu Cys Phe Arg Leu Ile Arg Gln His Thr
245 250 255
Val Thr Pro Ile Ala Val Gly Glu Val Phe Asn Ser Ile Trp Asp Cys
260 265 270
Lys Gln Leu Ile Glu Glu Gln Leu Ile Asp Tyr Ile Arg Thr Thr Leu
275 280 285
Thr His Ala Gly Gly Ile Thr Gly Met Arg Arg Ile Ala Asp Phe Ala
290 295 300
Ser Leu Tyr Gln Val Arg Thr Gly Ser His Gly Pro Ser Asp Leu Ser
305 310 315 320
Pro Val Cys Met Ala Ala Ala Leu His Phe Asp Leu Trp Val Pro Asn
325 330 335
Phe Gly Val Gln Glu Tyr Met Gly Tyr Ser Glu Gln Met Leu Glu Val
340 345 350
Phe Pro His Asn Trp Thr Phe Asp Asn Gly Tyr Met His Pro Gly Asp
355 360 365
Lys Pro Gly Leu Gly Ile Glu Phe Asp Glu Lys Leu Ala Ala Lys Tyr
370 375 380
Pro Tyr Glu Pro Ala Tyr Leu Pro Val Ala Arg Leu Glu Asp Gly Thr
385 390 395 400
Leu Trp Asn Arg
<210> 88
<211> 406
<212> PRT
<213> 福氏志贺氏菌
<400> 88
Met Thr Phe Ser Val Asp Lys Val Arg Ala Asp Phe Pro Val Leu Ser
1 5 10 15
Arg Glu Val Asn Gly Leu Pro Leu Ala Tyr Leu Asp Ser Ala Ala Ser
20 25 30
Ala Gln Lys Pro Ser Gln Val Ile Asp Ala Glu Ala Glu Phe Tyr Arg
35 40 45
His Gly Tyr Ala Ala Val His Arg Gly Ile His Thr Leu Ser Ala Gln
50 55 60
Ala Thr Glu Lys Met Glu Asn Val Arg Lys Arg Ala Ser Leu Phe Ile
65 70 75 80
Asn Ala Arg Ser Ala Glu Glu Leu Val Phe Val Arg Gly Thr Thr Glu
85 90 95
Gly Ile Asn Leu Val Ala Asn Ser Trp Gly Asn Ser Asn Val Arg Ala
100 105 110
Gly Asp Asn Ile Ile Ile Ser Gln Met Glu His His Ala Asn Ile Val
115 120 125
Pro Trp Gln Met Leu Cys Ala Arg Val Gly Ala Glu Leu Arg Val Ile
130 135 140
Pro Leu Asn Pro Asp Gly Thr Leu Gln Leu Glu Thr Leu Pro Thr Leu
145 150 155 160
Phe Asp Glu Lys Thr Arg Leu Leu Ala Ile Thr His Val Ser Asn Val
165 170 175
Leu Gly Thr Glu Asn Pro Leu Ala Glu Met Ile Thr Leu Ala His Gln
180 185 190
His Gly Ala Lys Val Leu Val Asp Gly Ala Gln Ala Val Met His His
195 200 205
Pro Val Asp Val Gln Ala Leu Asp Cys Asp Phe Tyr Val Phe Ser Gly
210 215 220
His Lys Leu Tyr Gly Pro Thr Gly Ile Gly Ile Leu Tyr Val Lys Glu
225 230 235 240
Ala Leu Leu Gln Glu Met Pro Pro Trp Glu Gly Gly Gly Ser Met Ile
245 250 255
Ala Thr Val Ser Leu Ser Glu Gly Thr Thr Trp Thr Lys Ala Pro Trp
260 265 270
Arg Phe Glu Ala Gly Thr Pro Asn Thr Gly Gly Ile Ile Gly Leu Gly
275 280 285
Ala Ala Leu Glu Tyr Val Ser Ala Leu Gly Leu Asn Ser Ile Ala Glu
290 295 300
Tyr Glu Gln Asn Leu Met His Tyr Ala Leu Ser Gln Leu Glu Ser Val
305 310 315 320
Pro Asp Leu Thr Leu Tyr Gly Gln Gln Asn Arg Leu Gly Val Ile Ala
325 330 335
Phe Asn Leu Gly Lys His His Ala Tyr Asp Val Gly Ser Phe Leu Asp
340 345 350
Asn Tyr Gly Ile Ala Val Arg Thr Gly His His Cys Ala Met Pro Leu
355 360 365
Met Ala Tyr Tyr Asn Val Pro Ala Met Cys Arg Ala Ser Leu Ala Met
370 375 380
Tyr Asn Thr His Glu Glu Val Glu Arg Leu Val Thr Gly Leu Gln Arg
385 390 395 400
Ile His Arg Leu Leu Gly
405
<210> 89
<211> 491
<212> PRT
<213> 福氏志贺氏菌
<400> 89
Met Ala Val Thr Gln Thr Ala Gln Ala Cys Asp Leu Val Ile Phe Gly
1 5 10 15
Ala Lys Gly Asp Leu Ala Arg Arg Lys Leu Leu Pro Ser Leu Tyr Gln
20 25 30
Leu Glu Lys Val Gly Gln Leu Asn Pro Asp Thr Arg Ile Ile Gly Val
35 40 45
Gly Arg Ala Asp Trp Asp Lys Ala Ala Tyr Thr Lys Val Val Arg Glu
50 55 60
Ala Leu Glu Thr Phe Met Lys Glu Thr Ile Asp Glu Gly Leu Trp Asp
65 70 75 80
Thr Leu Ser Ala Arg Leu Asp Phe Cys Asn Leu Asp Val Asn Asp Thr
85 90 95
Ala Ala Phe Asn Arg Leu Gly Ala Met Leu Asp Gln Lys Asn Arg Ile
100 105 110
Thr Ile Asn Tyr Phe Ala Met Pro Pro Ser Thr Phe Gly Ala Ile Cys
115 120 125
Lys Gly Leu Gly Glu Ala Lys Leu Asn Ala Lys Pro Ala Arg Val Val
130 135 140
Met Glu Lys Pro Leu Gly Thr Ser Leu Ala Thr Ser Gln Glu Ile Asn
145 150 155 160
Asp Gln Val Gly Glu Tyr Phe Glu Glu Cys Gln Val Tyr Arg Ile Asp
165 170 175
His Tyr Leu Gly Lys Glu Thr Val Leu Asn Leu Leu Ala Leu Arg Phe
180 185 190
Ala Asn Ser Leu Phe Val Asn Asn Trp Asp Asn Arg Thr Ile Asp His
195 200 205
Val Glu Ile Thr Val Ala Glu Glu Val Gly Ile Glu Gly Arg Trp Gly
210 215 220
Tyr Phe Asp Lys Ala Gly Gln Met Arg Asp Met Ile Gln Asn His Leu
225 230 235 240
Leu Gln Ile Leu Cys Met Ile Ala Met Ser Pro Pro Ser Asp Leu Ser
245 250 255
Ala Asp Ser Ile Arg Asp Glu Lys Val Lys Val Leu Lys Ser Leu Arg
260 265 270
Arg Ile Asp Arg Ser Asn Val Arg Glu Lys Thr Val Arg Gly Gln Tyr
275 280 285
Thr Ala Gly Phe Ala Gln Gly Lys Lys Val Pro Gly Tyr Leu Glu Glu
290 295 300
Glu Gly Ala Asn Lys Ser Ser Asn Thr Glu Thr Phe Val Ala Ile Arg
305 310 315 320
Val Asp Ile Asp Asn Trp Arg Trp Ala Gly Val Pro Phe Tyr Leu Arg
325 330 335
Thr Gly Lys Arg Leu Pro Thr Lys Cys Ser Glu Val Val Val Tyr Phe
340 345 350
Lys Thr Pro Glu Leu Asn Leu Phe Lys Glu Ser Trp Gln Asp Leu Pro
355 360 365
Gln Asn Lys Leu Thr Ile Arg Leu Gln Pro Asp Glu Gly Val Asp Ile
370 375 380
Gln Val Leu Asn Lys Val Pro Gly Leu Asp His Lys His Asn Leu Gln
385 390 395 400
Ile Thr Lys Leu Asp Leu Ser Tyr Ser Glu Thr Phe Asn Gln Thr His
405 410 415
Leu Ala Asp Ala Tyr Glu Arg Leu Leu Leu Glu Thr Met Arg Gly Ile
420 425 430
Gln Ala Leu Phe Val Arg Arg Asp Glu Val Glu Glu Ala Trp Lys Trp
435 440 445
Val Asp Ser Ile Thr Glu Ala Trp Ala Met Asp Asn Asp Ala Pro Lys
450 455 460
Pro Tyr Gln Ala Gly Thr Trp Gly Pro Val Ala Ser Val Ala Met Ile
465 470 475 480
Thr Arg Asp Gly Arg Ser Trp Asn Glu Phe Glu
485 490
<210> 90
<211> 590
<212> PRT
<213> 福氏志贺氏菌
<400> 90
Met Arg Thr Glu Tyr Cys Gly Gln Leu Arg Leu Ser His Val Gly Gln
1 5 10 15
Gln Val Thr Leu Cys Gly Trp Val Asn Arg Arg Arg Asp Leu Gly Ser
20 25 30
Leu Ile Phe Ile Asp Met Arg Asp Arg Glu Gly Ile Val Gln Val Phe
35 40 45
Phe Asp Pro Asp Arg Ala Asp Ala Leu Lys Leu Ala Ser Glu Leu Arg
50 55 60
Asn Glu Phe Cys Ile Gln Val Thr Gly Thr Val Arg Ala Arg Asp Glu
65 70 75 80
Lys Asn Ile Asn Arg Asp Met Ala Thr Gly Glu Ile Glu Val Leu Ala
85 90 95
Ser Ser Leu Thr Ile Ile Asn Arg Ala Asp Val Leu Pro Leu Asp Ser
100 105 110
Asn His Val Asn Thr Glu Glu Ala Arg Leu Lys Tyr Arg Tyr Leu Asp
115 120 125
Leu Arg Arg Pro Glu Met Ala Gln Arg Leu Lys Thr Arg Ala Lys Ile
130 135 140
Thr Ser Leu Val Arg Arg Phe Met Asp Asp His Gly Phe Leu Asp Ile
145 150 155 160
Glu Thr Pro Met Leu Thr Lys Ala Thr Pro Glu Gly Ala Arg Asp Tyr
165 170 175
Leu Val Pro Ser Arg Val His Lys Gly Lys Phe Tyr Ala Leu Pro Gln
180 185 190
Ser Pro Gln Leu Phe Lys Gln Leu Leu Met Met Ser Gly Phe Asp Arg
195 200 205
Tyr Tyr Gln Ile Val Lys Cys Phe Arg Asp Glu Asp Leu Arg Ala Asp
210 215 220
Arg Gln Pro Glu Phe Thr Gln Ile Asp Val Glu Thr Ser Phe Met Thr
225 230 235 240
Ala Pro Gln Val Arg Glu Val Met Glu Ala Leu Val Arg His Leu Trp
245 250 255
Leu Glu Val Lys Gly Val Asp Leu Gly Asp Phe Pro Val Met Thr Phe
260 265 270
Ala Glu Ala Glu Arg Arg Tyr Gly Ser Asp Lys Pro Asp Leu Arg Asn
275 280 285
Pro Met Glu Leu Thr Asp Val Ala Asp Leu Leu Lys Ser Val Glu Phe
290 295 300
Ala Val Phe Ala Gly Pro Ala Asn Asp Pro Lys Gly Arg Val Ala Ala
305 310 315 320
Leu Arg Val Pro Gly Gly Ala Ser Leu Thr Arg Lys Gln Ile Asp Glu
325 330 335
Tyr Gly Asn Phe Val Lys Ile Tyr Gly Ala Lys Gly Leu Ala Tyr Ile
340 345 350
Lys Val Asn Glu Arg Ala Lys Gly Leu Glu Gly Ile Asn Ser Pro Val
355 360 365
Ala Lys Phe Leu Asn Ala Glu Ile Ile Glu Ala Ile Leu Asp Arg Thr
370 375 380
Ala Ala Gln Asp Gly Asp Met Ile Phe Phe Gly Ala Asp Asn Lys Lys
385 390 395 400
Ile Val Ala Asp Gly Met Gly Ala Leu Leu Leu Lys Val Gly Lys Asp
405 410 415
Leu Gly Leu Thr Asp Glu Ser Lys Trp Ala Pro Leu Trp Val Ile Asp
420 425 430
Phe Pro Met Leu Gly Asp Asp Gly Glu Gly Gly Leu Thr Ala Met His
435 440 445
His Pro Phe Thr Ser Pro Lys Asp Met Thr Ala Ala Glu Leu Lys Ala
450 455 460
Ala Pro Glu Asn Ala Val Ala Asn Ala Tyr Asp Met Val Ile Asn Gly
465 470 475 480
Tyr Glu Val Gly Gly Gly Ser Val Arg Ile His Asn Gly Asp Met Gln
485 490 495
Gln Thr Val Phe Gly Ile Leu Gly Ile Asn Glu Glu Glu Gln Arg Glu
500 505 510
Lys Phe Gly Phe Leu Leu Asp Ala Leu Lys Tyr Gly Thr Pro Pro His
515 520 525
Ala Gly Leu Ala Phe Gly Leu Asp Arg Leu Thr Met Leu Leu Thr Gly
530 535 540
Thr Asp Asn Ile Arg Asp Val Ile Ala Phe Pro Lys Thr Thr Ala Ala
545 550 555 560
Ala Cys Leu Met Thr Glu Ala Pro Ser Phe Ala Asn Pro Thr Ala Leu
565 570 575
Ala Glu Leu Ser Ile Gln Val Val Lys Lys Ala Glu Asn Asn
580 585 590
<210> 91
<211> 296
<212> PRT
<213> 福氏志贺氏菌
<400> 91
Met Leu Leu Gly Cys Leu Ala Leu Thr Cys Ser Ile Ala Phe Gln Ala
1 5 10 15
Ser Ala Thr Glu Lys Phe Lys Val Ile Thr Thr Phe Thr Ile Ile Ala
20 25 30
Asp Met Ala Lys Asn Val Ala Gly Asp Ala Ala Glu Val Ser Ser Ile
35 40 45
Thr Lys Pro Gly Ala Glu Ile His Glu Tyr Gln Pro Thr Pro Gly Asp
50 55 60
Ile Lys Arg Ala Gln Gly Ala Gln Leu Ile Leu Ala Asn Gly Met Asn
65 70 75 80
Leu Glu Leu Trp Phe Gln Arg Phe Tyr Gln His Leu Asn Gly Val Pro
85 90 95
Glu Val Ile Val Ser Ser Gly Val Thr Pro Val Gly Ile Thr Glu Gly
100 105 110
Pro Tyr Glu Gly Lys Pro Asn Pro His Ala Trp Met Ser Pro Asp Asn
115 120 125
Ala Leu Ile Tyr Val Asp Asn Ile Arg Asp Ala Leu Ile Lys Tyr Asp
130 135 140
Pro Ala Asn Ala Gln Thr Tyr Gln Arg Asn Ala Asp Thr Tyr Lys Ala
145 150 155 160
Lys Ile Thr Gln Thr Leu Ala Pro Leu Arg Lys Gln Ile Thr Glu Leu
165 170 175
Pro Glu Asn Gln Arg Trp Met Val Thr Ser Glu Gly Ala Phe Ser Tyr
180 185 190
Leu Ala Arg Asp Leu Gly Leu Lys Glu Leu Tyr Leu Trp Pro Ile Asn
195 200 205
Ala Asp Gln Gln Gly Thr Pro Gln Gln Val Arg Lys Val Val Asp Ile
210 215 220
Val Lys Lys Asn His Ile Pro Ala Val Phe Ser Glu Ser Thr Ile Ser
225 230 235 240
Asp Lys Pro Ala Arg Gln Val Ala Arg Glu Thr Gly Ala His Tyr Gly
245 250 255
Gly Val Leu Tyr Val Asp Ser Leu Ser Thr Glu Asn Gly Pro Val Pro
260 265 270
Thr Tyr Ile Asp Leu Leu Lys Val Thr Thr Ser Thr Leu Val Gln Gly
275 280 285
Ile Lys Ala Gly Lys Arg Glu Lys
290 295
<210> 92
<211> 131
<212> PRT
<213> 福氏志贺氏菌
<400> 92
Met Leu Ala Leu Thr Asn Ser Gly Cys Leu Asn Glu Ser Asp Ser His
1 5 10 15
Ile Ile Arg Gly Ile Lys Met Glu Thr Thr Lys Pro Ser Phe Gln Asp
20 25 30
Val Leu Glu Phe Val Arg Leu Phe Arg Arg Lys Asn Lys Leu Gln Arg
35 40 45
Glu Ile Gln Asp Val Glu Lys Lys Ile Arg Asp Asn Gln Lys Arg Val
50 55 60
Leu Leu Leu Asp Asn Leu Ser Asp Tyr Ile Lys Pro Gly Met Ser Val
65 70 75 80
Glu Ala Ile Gln Gly Ile Ile Ala Ser Met Lys Gly Asp Tyr Glu Asp
85 90 95
Arg Val Asp Asp Tyr Ile Ile Lys Asn Ala Glu Leu Ser Lys Glu Arg
100 105 110
Arg Asp Ile Ser Lys Lys Leu Lys Ala Met Gly Glu Met Lys Asn Gly
115 120 125
Glu Ala Lys
130
<210> 93
<211> 355
<212> PRT
<213> 福氏志贺氏菌
<400> 93
Met Ser Gln Lys Tyr Leu Phe Ile Asp Arg Asp Gly Thr Leu Ile Ser
1 5 10 15
Glu Pro Pro Ser Asp Phe Gln Val Asp Arg Phe Asp Lys Leu Ala Phe
20 25 30
Glu Pro Gly Val Ile Pro Glu Leu Leu Lys Leu Gln Lys Ala Gly Tyr
35 40 45
Lys Leu Val Met Ile Thr Asn Gln Asp Gly Leu Gly Thr Gln Ser Phe
50 55 60
Pro Gln Ala Asp Phe Asp Gly Pro His Asn Leu Met Met Gln Ile Phe
65 70 75 80
Thr Ser Gln Gly Val Gln Phe Asp Glu Val Leu Ile Cys Pro His Leu
85 90 95
Pro Ala Asp Glu Cys Asp Cys Arg Lys Pro Lys Val Lys Leu Val Glu
100 105 110
Gly Tyr Leu Ala Glu Gln Ala Met Asp Arg Ala Asn Ser Tyr Val Ile
115 120 125
Gly Asp Arg Ala Thr Asp Ile Gln Leu Ala Glu Asn Met Gly Ile Asn
130 135 140
Gly Leu Arg Tyr Asp Arg Glu Thr Leu Asn Trp Pro Met Ile Gly Glu
145 150 155 160
Gln Leu Thr Lys Arg Asp Arg Tyr Ala His Val Val Arg Asn Thr Lys
165 170 175
Glu Thr Gln Ile Asp Val Gln Val Trp Leu Asp Arg Glu Gly Gly Ser
180 185 190
Lys Ile Asn Thr Gly Val Gly Phe Phe Asp His Met Leu Asp Gln Ile
195 200 205
Ala Thr His Gly Gly Phe Arg Met Glu Ile Asn Val Lys Gly Asp Leu
210 215 220
Tyr Ile Asp Asp His His Thr Val Glu Asp Thr Gly Leu Ala Leu Gly
225 230 235 240
Glu Ala Leu Lys Ile Ala Leu Gly Asp Lys Arg Gly Ile Cys Arg Phe
245 250 255
Gly Phe Val Leu Pro Met Asp Glu Cys Leu Ala Arg Cys Ala Leu Asp
260 265 270
Ile Ser Gly Arg Pro His Leu Glu Tyr Lys Ala Glu Phe Thr Tyr Gln
275 280 285
Arg Val Gly Asp Leu Ser Thr Glu Met Ile Glu His Phe Phe Arg Ser
290 295 300
Leu Ser Tyr Thr Met Gly Val Thr Leu His Leu Lys Thr Lys Gly Lys
305 310 315 320
Asn Asp His His Arg Val Glu Ser Leu Phe Lys Ala Phe Gly Arg Thr
325 330 335
Leu Arg Gln Ala Ile Arg Val Glu Gly Asp Thr Leu Pro Ser Ser Lys
340 345 350
Gly Val Leu
355
<210> 94
<211> 332
<212> PRT
<213> 福氏志贺氏菌
<400> 94
Met Asn Lys Lys Val Leu Thr Leu Ser Ala Val Met Ala Ser Met Leu
1 5 10 15
Phe Gly Ala Ala Ala His Ala Ala Asp Thr Arg Ile Gly Val Thr Ile
20 25 30
Tyr Lys Tyr Asp Asp Asn Phe Met Ser Val Val Arg Lys Ala Ile Glu
35 40 45
Gln Asp Ala Lys Ala Ala Pro Asp Val Gln Leu Leu Met Asn Asp Ser
50 55 60
Gln Asn Asp Gln Ser Lys Gln Asn Asp Gln Ile Asp Val Leu Leu Ala
65 70 75 80
Lys Gly Val Lys Ala Leu Ala Ile Asn Leu Val Asp Pro Ala Ala Ala
85 90 95
Gly Thr Val Ile Glu Lys Ala Arg Gly Gln Asn Val Pro Val Val Phe
100 105 110
Phe Asn Lys Glu Pro Ser Arg Lys Ala Leu Asp Ser Tyr Asp Lys Ala
115 120 125
Tyr Tyr Val Gly Thr Asp Ser Lys Glu Ser Gly Ile Ile Gln Gly Asp
130 135 140
Leu Ile Ala Lys His Trp Ala Ala Asn Gln Gly Trp Asp Leu Asn Lys
145 150 155 160
Asp Gly Gln Ile Gln Phe Val Leu Leu Lys Gly Glu Pro Gly His Pro
165 170 175
Asp Ala Glu Ala Arg Thr Thr Tyr Val Ile Lys Glu Leu Asn Asp Lys
180 185 190
Gly Ile Lys Thr Glu Gln Leu Gln Leu Asp Thr Ala Met Trp Asp Thr
195 200 205
Ala Gln Ala Lys Asp Lys Met Asp Ala Trp Leu Ser Gly Pro Asn Ala
210 215 220
Asn Lys Ile Glu Val Val Ile Ala Asn Asn Asp Ala Met Ala Met Gly
225 230 235 240
Ala Val Glu Ala Leu Lys Ala His Asn Lys Ser Ser Ile Pro Val Phe
245 250 255
Gly Val Asp Ala Leu Pro Glu Ala Leu Ala Leu Val Lys Ser Gly Ala
260 265 270
Leu Ala Gly Thr Val Leu Asn Asp Ala Asn Asn Gln Ala Lys Ala Thr
275 280 285
Phe Asp Leu Ala Lys Asn Leu Ala Asp Gly Lys Gly Ala Ala Asp Gly
290 295 300
Thr Asn Trp Lys Ile Asp Asn Lys Val Val Arg Val Pro Tyr Val Gly
305 310 315 320
Val Asp Lys Asp Asn Leu Ala Glu Phe Ser Lys Lys
325 330
<210> 95
<211> 432
<212> PRT
<213> 福氏志贺氏菌
<400> 95
Met Ser Lys Ile Val Lys Ile Ile Gly Arg Glu Ile Ile Asp Ser Arg
1 5 10 15
Gly Asn Pro Thr Val Glu Ala Glu Val His Leu Glu Gly Gly Phe Val
20 25 30
Gly Met Ala Ala Ala Pro Ser Gly Ala Ser Thr Gly Ser Arg Glu Ala
35 40 45
Leu Glu Leu Arg Asp Gly Asp Lys Ser Arg Phe Leu Gly Lys Gly Val
50 55 60
Thr Lys Ala Val Ala Ala Val Asn Gly Pro Ile Ala Gln Ala Leu Ile
65 70 75 80
Gly Lys Asp Ala Lys Asp Gln Ala Gly Ile Asp Lys Ile Met Ile Asp
85 90 95
Leu Asp Gly Thr Glu Asn Lys Ser Lys Phe Gly Ala Asn Ala Ile Leu
100 105 110
Ala Val Ser Leu Ala Asn Ala Lys Ala Ala Ala Ala Ala Lys Gly Met
115 120 125
Pro Leu Tyr Glu His Ile Ala Glu Leu Asn Gly Thr Pro Gly Lys Tyr
130 135 140
Ser Met Pro Val Pro Met Met Asn Ile Ile Asn Gly Gly Glu His Ala
145 150 155 160
Asp Asn Asn Val Asp Ile Gln Glu Phe Met Ile Gln Pro Val Gly Ala
165 170 175
Lys Thr Val Lys Glu Ala Ile Arg Met Gly Ser Glu Val Phe His His
180 185 190
Leu Ala Lys Val Leu Lys Ala Lys Gly Met Asn Thr Ala Val Gly Asp
195 200 205
Glu Gly Gly Tyr Ala Pro Asn Leu Gly Ser Asn Ala Glu Ala Leu Ala
210 215 220
Val Ile Ala Glu Ala Val Lys Ala Ala Gly Tyr Glu Leu Gly Lys Asp
225 230 235 240
Ile Thr Leu Ala Met Asp Cys Ala Ala Ser Glu Phe Tyr Lys Asp Gly
245 250 255
Lys Tyr Val Leu Ala Gly Glu Gly Asn Lys Ala Phe Thr Ser Glu Glu
260 265 270
Phe Thr His Phe Leu Glu Glu Leu Thr Lys Gln Tyr Pro Ile Val Ser
275 280 285
Ile Glu Asp Gly Leu Asp Glu Ser Asp Trp Asp Gly Phe Ala Tyr Gln
290 295 300
Thr Lys Val Leu Gly Asp Lys Ile Gln Leu Val Gly Asp Asp Leu Phe
305 310 315 320
Val Thr Asn Thr Lys Ile Leu Lys Glu Gly Ile Glu Lys Gly Ile Ala
325 330 335
Asn Ser Ile Leu Ile Lys Phe Asn Gln Ile Gly Ser Leu Thr Glu Thr
340 345 350
Leu Ala Ala Ile Lys Met Ala Lys Asp Ala Gly Tyr Thr Ala Val Ile
355 360 365
Ser His Arg Ser Gly Glu Thr Glu Asp Ala Thr Ile Ala Asp Leu Ala
370 375 380
Val Gly Thr Ala Ala Gly Gln Ile Lys Thr Gly Ser Met Ser Arg Ser
385 390 395 400
Asp Arg Val Ala Lys Tyr Asn Gln Leu Ile Arg Ile Glu Glu Ala Leu
405 410 415
Gly Glu Lys Ala Pro Tyr Asn Gly Arg Lys Glu Ile Lys Gly Gln Ala
420 425 430
<210> 96
<211> 663
<212> PRT
<213> 福氏志贺氏菌
<400> 96
Met Ser Ser Arg Lys Glu Leu Ala Asn Ala Ile Arg Ala Leu Ser Met
1 5 10 15
Asp Ala Val Gln Lys Ala Lys Ser Gly His Pro Gly Ala Pro Met Gly
20 25 30
Met Ala Asp Ile Ala Glu Val Leu Trp Arg Asp Phe Leu Lys His Asn
35 40 45
Pro Gln Asn Pro Ser Trp Ala Asp Arg Asp Arg Phe Val Leu Ser Asn
50 55 60
Gly His Gly Ser Met Leu Ile Tyr Ser Leu Leu His Leu Thr Gly Tyr
65 70 75 80
Asp Leu Pro Met Glu Glu Leu Lys Asn Phe Arg Gln Leu His Ser Lys
85 90 95
Thr Pro Gly His Pro Glu Val Gly Tyr Thr Ala Gly Val Glu Thr Thr
100 105 110
Thr Gly Pro Leu Gly Gln Gly Ile Ala Asn Ala Val Gly Met Ala Ile
115 120 125
Ala Glu Lys Thr Leu Ala Ala Gln Phe Asn Arg Pro Gly His Asp Ile
130 135 140
Val Asp His Tyr Thr Tyr Ala Phe Met Gly Asp Gly Cys Met Met Glu
145 150 155 160
Gly Ile Ser His Glu Val Cys Ser Leu Ala Gly Thr Leu Lys Leu Gly
165 170 175
Lys Leu Ile Ala Phe Tyr Asp Asp Asn Gly Ile Ser Ile Asp Gly His
180 185 190
Val Glu Gly Trp Phe Thr Asp Asp Thr Ala Met Arg Phe Glu Ala Tyr
195 200 205
Gly Trp His Val Ile Arg Asp Ile Asp Gly His Asp Ala Ala Ser Ile
210 215 220
Lys Arg Ala Val Glu Glu Ala Arg Ala Val Thr Asp Lys Pro Ser Leu
225 230 235 240
Leu Met Cys Lys Thr Ile Ile Gly Phe Gly Ser Pro Asn Lys Ala Gly
245 250 255
Thr His Asp Ser His Gly Ala Pro Leu Gly Asp Ala Glu Ile Ala Leu
260 265 270
Thr Arg Glu Gln Leu Gly Trp Lys Tyr Ala Pro Phe Glu Ile Pro Ser
275 280 285
Glu Ile Tyr Ala Gln Trp Asp Ala Lys Glu Ala Gly Gln Ala Lys Glu
290 295 300
Ser Ala Trp Asn Glu Lys Phe Ala Ala Tyr Ala Lys Ala Tyr Pro Gln
305 310 315 320
Glu Ala Ala Glu Phe Thr Arg Arg Met Lys Gly Glu Met Pro Ser Asp
325 330 335
Phe Asp Ala Lys Ala Lys Glu Phe Ile Ala Lys Leu Gln Ala Asn Pro
340 345 350
Ala Lys Ile Ala Ser Arg Lys Ala Ser Gln Asn Ala Ile Glu Ala Phe
355 360 365
Gly Pro Leu Leu Pro Glu Phe Leu Gly Gly Ser Ala Asp Leu Ala Pro
370 375 380
Ser Asn Leu Thr Leu Trp Ser Gly Ser Lys Ala Ile Asn Glu Asp Ala
385 390 395 400
Ala Gly Asn Tyr Ile His Tyr Gly Val Arg Glu Phe Gly Met Thr Ala
405 410 415
Ile Ala Asn Gly Ile Ser Leu His Gly Gly Phe Leu Pro Tyr Thr Ser
420 425 430
Thr Phe Leu Met Phe Val Glu Tyr Ala Arg Asn Ala Val Arg Met Ala
435 440 445
Ala Leu Met Lys Gln Arg Gln Val Met Val Tyr Thr His Asp Ser Ile
450 455 460
Gly Leu Gly Glu Asp Gly Pro Thr His Gln Pro Val Glu Gln Val Ala
465 470 475 480
Ser Leu Arg Val Thr Pro Asn Met Ser Thr Trp Arg Pro Cys Asp Gln
485 490 495
Val Glu Ser Ala Val Ala Trp Lys Tyr Gly Val Glu Arg Gln Asp Gly
500 505 510
Pro Thr Ala Leu Ile Leu Ser Arg Gln Asn Leu Ala Gln Gln Glu Arg
515 520 525
Thr Glu Glu Gln Leu Ala Asn Ile Ala Arg Gly Gly Tyr Val Leu Lys
530 535 540
Asp Cys Ala Gly Gln Pro Glu Leu Ile Phe Ile Ala Thr Gly Ser Glu
545 550 555 560
Val Glu Leu Ala Val Ala Ala Tyr Glu Lys Leu Thr Ala Glu Gly Val
565 570 575
Lys Ala Arg Val Val Ser Met Pro Ser Thr Asp Ala Phe Asp Lys Gln
580 585 590
Asp Ala Ala Tyr Arg Glu Ser Val Leu Pro Lys Ala Val Thr Ala Arg
595 600 605
Val Ala Val Glu Ala Gly Ile Ala Asp Tyr Trp Tyr Lys Tyr Val Gly
610 615 620
Leu Asn Gly Ala Ile Val Gly Met Thr Thr Phe Gly Glu Ser Ala Pro
625 630 635 640
Ala Glu Leu Leu Phe Glu Glu Phe Gly Phe Thr Val Asp Asn Val Val
645 650 655
Ala Lys Ala Lys Ala Leu Leu
660
<210> 97
<211> 348
<212> PRT
<213> 福氏志贺氏菌
<400> 97
Met Glu Phe Phe Lys Lys Thr Ala Leu Ala Ala Leu Val Met Gly Phe
1 5 10 15
Ser Gly Ala Ala Leu Ala Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly
20 25 30
Gly Thr Ile Ala Gly Gly Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr
35 40 45
Ala Gly Lys Val Gly Val Glu Asn Leu Val Asn Ala Val Pro Gln Leu
50 55 60
Lys Asp Ile Ala Asn Val Lys Gly Glu Gln Val Val Asn Ile Gly Ser
65 70 75 80
Gln Asp Met Asn Asp Asn Val Trp Leu Thr Leu Ala Lys Lys Ile Asn
85 90 95
Ala Asp Cys Asp Lys Thr Asp Gly Phe Val Ile Thr His Gly Thr Asp
100 105 110
Thr Met Glu Glu Thr Ala Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp
115 120 125
Lys Pro Val Val Met Val Gly Ala Met Arg Pro Ser Thr Ser Met Ser
130 135 140
Ala Asp Gly Pro Phe Asn Leu Tyr Asn Ala Val Val Thr Ala Ala Asp
145 150 155 160
Lys Ala Ser Ala Asn Arg Gly Val Leu Val Val Met Asn Asp Thr Val
165 170 175
Leu Asp Gly Arg Asp Val Thr Lys Thr Asn Thr Thr Asp Val Ala Thr
180 185 190
Phe Lys Ser Val Asn Tyr Gly Pro Leu Gly Tyr Ile His Asn Gly Lys
195 200 205
Ile Asp Tyr Gln Arg Thr Pro Ala Arg Lys His Thr Ser Asp Thr Pro
210 215 220
Phe Asp Val Ser Lys Leu Asn Glu Leu Pro Lys Val Gly Ile Val Tyr
225 230 235 240
Asn Tyr Ala Asn Ala Ser Asp Leu Pro Ala Lys Ala Leu Val Asp Ala
245 250 255
Gly Tyr Asp Gly Ile Val Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr
260 265 270
Lys Ser Val Phe Asp Thr Leu Ala Thr Ala Ala Lys Asn Gly Thr Ala
275 280 285
Val Val Arg Ser Ser Arg Val Pro Thr Gly Ala Thr Thr Gln Asp Ala
290 295 300
Glu Val Asp Asp Ala Lys Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn
305 310 315 320
Pro Gln Lys Ala Arg Val Leu Leu Gln Leu Ala Leu Thr Gln Thr Lys
325 330 335
Asp Pro Gln Gln Ile Gln Gln Ile Phe Asn Gln Tyr
340 345
<210> 98
<211> 1171
<212> PRT
<213> 福氏志贺氏菌
<400> 98
Met Asp Glu Asn Ala Leu Gly Phe Ala Ser Tyr Trp Arg Asn Ser Leu
1 5 10 15
Ala Asp Ala Glu Ser Gly Lys Gly Ser Phe Lys Arg Lys Asp Ala Gln
20 25 30
Asn Phe Thr His Trp His Gly Ile Ala Ala Gly Arg Leu Asp Glu Ala
35 40 45
Ile Val Ser Lys Phe Phe Glu Gly Glu Lys Asp Asp Val Glu Thr Val
50 55 60
Asp Val Ile Leu Arg Pro Lys Val Tyr Phe Arg Leu Leu Gln His Gly
65 70 75 80
Lys Asp Arg Ser Ala Gly Ala Pro Asp Ile Val Thr Pro Ile Val Thr
85 90 95
Pro Ala Leu Leu Ser Arg Glu Gly Phe Leu Tyr Pro Thr Pro Ala Thr
100 105 110
Ser Ile Pro Arg Asp Leu Leu Glu Pro Leu Pro Lys Gly Ala Phe Ser
115 120 125
Ile Gly Glu Ile Gly Gln Tyr Asp Lys Tyr Lys Thr Thr His Thr Thr
130 135 140
Phe Ser Ile Asn Phe Asp Asp Ser Val Asp Lys Thr Ala Glu Thr Asp
145 150 155 160
Glu Glu Arg Glu Ala Arg Tyr Ala Ala Leu Gln Gln Glu Trp Arg Gln
165 170 175
Tyr Leu Tyr Asp Ser Glu Arg Leu Leu Lys Ser Val Ala Gly Asp Trp
180 185 190
Ile Glu Lys Pro Glu Gln Tyr Glu Leu Ala Glu His Gly Tyr Ile Val
195 200 205
Lys Thr Ala Gln Ser Gly Gly Ala Ser Ser His Ile Leu Ser Leu Tyr
210 215 220
Asp His Leu Leu Val Cys Asn Lys Asp Val Pro Leu Phe Asn Arg Phe
225 230 235 240
Ala Ser Arg Glu Val His Ala Ala Glu Ser Leu Leu Ala Pro Gly Ala
245 250 255
Lys Phe Ser Asp Arg Leu Gly His Ser Gly Asp Lys Phe Pro Leu Ala
260 265 270
Lys Ala Gln Arg Asp Ala Leu Ser His Phe Leu Asp Ala Arg His Gly
275 280 285
Asp Ile Leu Ala Val Asn Gly Pro Pro Gly Thr Gly Lys Thr Thr Leu
290 295 300
Val Leu Ser Ile Ile Ala Thr Gln Trp Ala Arg Ala Ala Leu Glu Lys
305 310 315 320
Ser Glu Pro Pro Val Ile Ile Ala Thr Ser Thr Asn Asn Gln Ala Val
325 330 335
Thr Asn Ile Ile Glu Ala Phe Gly Lys Asp Phe Ser Gln Gly Ser Gly
340 345 350
Ala Met Ala Gly Arg Trp Leu Pro Glu Leu Lys Ser Phe Gly Ala Tyr
355 360 365
Phe Pro Ser Ser Ser Arg Lys Ala Glu Ala Ala Lys Lys Tyr Gln Thr
370 375 380
Glu Asp Phe Phe Asn Gln Val Glu Ser Lys Glu Tyr Val Glu Asp Ala
385 390 395 400
Leu Leu Phe Tyr Leu Glu Lys Ala Lys Ala Ala Phe Pro Gly Lys Glu
405 410 415
Cys Ser Ser Pro Glu Lys Val Ile Glu Leu Leu His Gly Gln Leu Ala
420 425 430
Ala Lys Ser Glu Gln Leu Ile Arg Leu Asn Ala Thr Trp Gln Thr Leu
435 440 445
Ser Gln Ile Arg Ala Ala Arg Glu Leu Ile Ala Asn Asp Ile Glu Gln
450 455 460
Tyr Leu Asp Asn Leu Asn Lys Leu Leu Ser Gly Gln Glu Gln Lys Val
465 470 475 480
Thr Leu Leu Lys Ser Ala Lys Thr Glu Trp Lys Lys Tyr Arg Ala Gly
485 490 495
Glu Ser Leu Ile Tyr Ser Leu Phe Ser Trp Leu Pro Ala Val Arg Asn
500 505 510
Lys Arg Gln Tyr Gln Ile Gln Leu Phe Leu Glu Asp Lys Leu Gly Ala
515 520 525
Leu Ile Ala Gly Asn Gln Trp Ser Asp Pro Glu Thr Ile Glu Arg Asn
530 535 540
Ile Asp Gly Leu Leu Asn Ser Ala Glu Arg Glu Gln Thr Thr Tyr Arg
545 550 555 560
Gln Gln Ile Asp Ser Ala His Glu Ile Val Leu Lys Glu Gln Gln Ala
565 570 575
Val Gln Glu Trp Gln Arg Leu Ala Phe Asp Leu Gly Tyr Glu Gly Asp
580 585 590
Glu Glu Leu Ser Phe Ser Gln Ala Asp Glu Leu Ala Asp Thr Gln Ile
595 600 605
Arg Phe Pro Ala Phe Leu Leu Thr Thr His Tyr Trp Glu Gly Arg Trp
610 615 620
Leu Met Asp Met Ala Ser Ile Asp Asp Leu Gln Asp Glu Lys Lys Lys
625 630 635 640
Lys Gly Ala Lys Gly Val Thr Ala Arg Trp Gln Arg Arg Met Lys Leu
645 650 655
Thr Pro Cys Val Val Met Thr Cys Tyr Met Leu Pro Gly Asn Met Gln
660 665 670
Ile Ser Glu His Lys Gly Gln Arg Lys Phe Glu Lys Ser Tyr Leu Tyr
675 680 685
Asp Phe Ala Asp Leu Leu Ile Val Asp Glu Ala Gly Gln Val Leu Pro
690 695 700
Glu Val Ala Ala Ala Ser Phe Ala Leu Ala Lys Lys Ala Leu Val Ile
705 710 715 720
Gly Asp Thr Glu Gln Ile Pro Pro Ile Trp Ser Ile Ala Pro Ala Ile
725 730 735
Asp Val Gly Asn Met Leu Ala Glu Lys Ile Leu Ser Gly Ser Thr Gln
740 745 750
Glu Glu Ile Thr Glu Lys Tyr Thr Ala Ile Ala Asp Leu Gly Lys Ser
755 760 765
Ala Ala Ser Gly Ser Val Met Lys Ile Ala Gln Phe Ala Ser Arg Tyr
770 775 780
Gln Tyr Asp Pro Glu Leu Ala Arg Gly Met Tyr Leu Tyr Glu His Arg
785 790 795 800
Arg Cys Tyr Asp Asn Ile Ile Gly Tyr Cys Asn Thr Leu Cys Tyr His
805 810 815
Gly Lys Leu Leu Pro Lys Arg Gly Arg Glu Glu Ser Asn Leu Met Pro
820 825 830
Ala Met Gly Tyr Leu His Ile Asp Gly Lys Gly Glu Leu Ala Ser Ser
835 840 845
Gly Ser Arg Tyr Asn Leu Leu Glu Ala Glu Thr Ile Ala Val Trp Leu
850 855 860
Ala Glu Asn Gln Gln Asn Ile Glu Ala His Tyr Gly Lys Ser Leu His
865 870 875 880
Glu Val Val Gly Ile Val Thr Pro Phe Ser Ala Gln Val Ser Thr Ile
885 890 895
Lys Gln Val Leu Gly Lys Gln Asp Ile Ser Thr Gly Thr Asn Glu Lys
900 905 910
Ser Leu Thr Val Gly Thr Val His Ser Leu Gln Gly Ala Glu Arg Ala
915 920 925
Ile Val Ile Phe Ser Pro Val Tyr Ser Lys His Glu Asp Gly Gly Phe
930 935 940
Ile Asp Ser Asp Asn Ser Met Leu Asn Val Ala Val Ser Arg Ala Lys
945 950 955 960
Asp Ser Phe Leu Val Phe Gly Asp Met Asp Leu Phe Glu Val Gln Pro
965 970 975
Ala Ser Ser Pro Arg Gly Leu Leu Ala Lys Tyr Leu Phe Glu Ser Glu
980 985 990
Lys Asn Ala Leu Ser Phe Asp Tyr Lys Glu Arg Lys Asp Leu Lys Thr
995 1000 1005
Ala Gly Thr Lys Ile Tyr Thr Leu His Gly Val Glu Gln His Asp Asn
1010 1015 1020
Phe Leu Asn Gln Thr Phe Glu Asn Thr Ser Lys His Ile Thr Ile Ile
1025 1030 1035 1040
Ser Pro Trp Leu Thr Trp Gln Arg Leu Glu Gln Thr Gly Phe Leu Asp
1045 1050 1055
Ser Met Ile Ala Ala Cys Ser Arg Gly Ile Asn Val Thr Ile Val Thr
1060 1065 1070
Asp Arg Ser Tyr Asn Thr Glu His Asn Asp Phe Glu Lys Arg Lys Glu
1075 1080 1085
Lys Gln Gln Asn Phe Lys Ala Ala Leu Glu Lys Leu Asn Ala Leu Gly
1090 1095 1100
Ile Ala Thr Lys Leu Val Asn Arg Val His Ser Lys Ile Val Ile Gly
1105 1110 1115 1120
Asp Asp Gly Leu Leu Cys Val Gly Ser Phe Asn Trp Phe Ser Ala Thr
1125 1130 1135
Arg Glu Ala Arg Tyr Glu Arg Tyr Asp Thr Ser Met Val Tyr Cys Gly
1140 1145 1150
Asp Asn Leu Lys Gly Glu Ile Glu Ala Ile Tyr Asn Ser Leu Glu Arg
1155 1160 1165
Arg Gln Val
1170
<210> 99
<211> 294
<212> PRT
<213> 福氏志贺氏菌
<400> 99
Met Lys Pro Phe Leu Arg Trp Cys Phe Val Ala Thr Ala Leu Thr Leu
1 5 10 15
Ala Gly Cys Ser Asn Thr Ser Trp Arg Lys Ser Glu Val Leu Ala Val
20 25 30
Pro Leu Gln Pro Thr Leu Gln Gln Glu Val Ile Leu Ala Arg Met Glu
35 40 45
Gln Ile Leu Ala Ser Arg Ala Leu Thr Asp Asp Glu Arg Ala Gln Leu
50 55 60
Leu Tyr Glu Arg Gly Val Leu Tyr Asp Ser Leu Gly Leu Arg Ala Leu
65 70 75 80
Ala Arg Asn Asp Phe Ser Gln Ala Leu Ala Ile Arg Pro Asp Met Pro
85 90 95
Glu Val Phe Asn Tyr Leu Gly Ile Tyr Leu Thr Gln Ala Gly Asn Phe
100 105 110
Asp Ala Ala Tyr Glu Ala Phe Asp Ser Val Leu Glu Leu Asp Pro Thr
115 120 125
Tyr Asn Tyr Ala His Leu Asn Arg Gly Ile Ala Leu Tyr Tyr Gly Gly
130 135 140
Arg Asp Lys Leu Ala Gln Asp Asp Leu Leu Ala Phe Tyr Gln Asp Asp
145 150 155 160
Pro Asn Asp Pro Phe Arg Ser Leu Trp Leu Tyr Leu Ala Glu Gln Lys
165 170 175
Leu Asp Glu Lys Gln Ala Lys Glu Val Leu Lys Gln His Phe Glu Lys
180 185 190
Ser Asp Lys Glu Gln Trp Gly Trp Asn Ile Val Glu Phe Tyr Leu Gly
195 200 205
Asn Ile Ser Glu Gln Thr Leu Met Glu Arg Leu Lys Ala Asp Ala Thr
210 215 220
Asp Asn Thr Ser Leu Ala Glu His Leu Ser Glu Thr Asn Phe Tyr Leu
225 230 235 240
Gly Lys Tyr Tyr Leu Ser Leu Gly Asp Leu Asp Ser Ala Thr Ala Leu
245 250 255
Phe Lys Leu Ala Val Ala Asn Asn Val His Asn Phe Val Glu His Arg
260 265 270
Tyr Ala Leu Leu Glu Leu Ser Leu Leu Gly Gln Asp Gln Asp Asp Leu
275 280 285
Ala Glu Ser Asp Gln Gln
290
<210> 100
<211> 1407
<212> PRT
<213> 福氏志贺氏菌
<400> 100
Met Lys Asp Leu Leu Lys Phe Leu Lys Ala Gln Thr Lys Thr Glu Glu
1 5 10 15
Phe Asp Ala Ile Lys Ile Ala Leu Ala Ser Pro Asp Met Ile Arg Ser
20 25 30
Trp Ser Phe Gly Glu Val Lys Lys Pro Glu Thr Ile Asn Tyr Arg Thr
35 40 45
Phe Lys Pro Glu Arg Asp Gly Leu Phe Cys Ala Arg Ile Phe Gly Pro
50 55 60
Val Lys Asp Tyr Glu Cys Leu Cys Gly Lys Tyr Lys Arg Leu Lys His
65 70 75 80
Arg Gly Val Ile Cys Glu Lys Cys Gly Val Glu Val Thr Gln Thr Lys
85 90 95
Val Arg Arg Glu Arg Met Gly His Ile Glu Leu Ala Ser Pro Thr Ala
100 105 110
His Ile Trp Phe Leu Lys Ser Leu Pro Ser Arg Ile Gly Leu Leu Leu
115 120 125
Asp Met Pro Leu Arg Asp Ile Glu Arg Val Leu Tyr Phe Glu Ser Tyr
130 135 140
Val Val Ile Glu Gly Gly Met Thr Asn Leu Glu Arg Gln Gln Ile Leu
145 150 155 160
Thr Glu Glu Gln Tyr Leu Asp Ala Leu Glu Glu Phe Gly Asp Glu Phe
165 170 175
Asp Ala Lys Met Gly Ala Glu Ala Ile Gln Ala Leu Leu Lys Ser Met
180 185 190
Asp Leu Glu Gln Glu Cys Glu Gln Leu Arg Glu Glu Leu Asn Glu Thr
195 200 205
Asn Ser Glu Thr Lys Arg Lys Lys Leu Thr Lys Arg Ile Lys Leu Leu
210 215 220
Glu Ala Phe Val Gln Ser Gly Asn Lys Pro Glu Trp Met Ile Leu Thr
225 230 235 240
Val Leu Pro Val Leu Pro Pro Asp Leu Arg Pro Leu Val Pro Leu Asp
245 250 255
Gly Gly Arg Phe Ala Thr Ser Asp Leu Asn Asp Leu Tyr Arg Arg Val
260 265 270
Ile Asn Arg Asn Asn Arg Leu Lys Arg Leu Leu Asp Leu Ala Ala Pro
275 280 285
Asp Ile Ile Val Arg Asn Glu Lys Arg Met Leu Gln Glu Ala Val Asp
290 295 300
Ala Leu Leu Asp Asn Gly Arg Arg Gly Arg Ala Ile Thr Gly Ser Asn
305 310 315 320
Lys Arg Pro Leu Lys Ser Leu Ala Asp Met Ile Lys Gly Lys Gln Gly
325 330 335
Arg Phe Arg Gln Asn Leu Leu Gly Lys Arg Val Asp Tyr Ser Gly Arg
340 345 350
Ser Val Ile Thr Val Gly Pro Tyr Leu Arg Leu His Gln Cys Gly Leu
355 360 365
Pro Lys Lys Met Ala Leu Glu Leu Phe Lys Pro Phe Ile Tyr Gly Lys
370 375 380
Leu Glu Leu Arg Gly Leu Ala Thr Thr Ile Lys Ala Ala Lys Lys Met
385 390 395 400
Val Glu Arg Glu Glu Ala Val Val Trp Asp Ile Leu Asp Glu Val Ile
405 410 415
Arg Glu His Pro Val Leu Leu Asn Arg Ala Pro Thr Leu His Arg Leu
420 425 430
Gly Ile Gln Ala Phe Glu Pro Val Leu Ile Glu Gly Lys Ala Ile Gln
435 440 445
Leu His Pro Leu Val Cys Ala Ala Tyr Asn Ala Asp Phe Asp Gly Asp
450 455 460
Gln Met Ala Val His Val Pro Leu Thr Leu Glu Ala Gln Leu Glu Ala
465 470 475 480
Arg Ala Leu Met Met Ser Thr Asn Asn Ile Leu Ser Pro Ala Asn Gly
485 490 495
Glu Pro Ile Ile Val Pro Ser Gln Asp Val Val Leu Gly Leu Tyr Tyr
500 505 510
Met Thr Arg Asp Cys Val Asn Ala Lys Gly Glu Gly Met Val Leu Thr
515 520 525
Gly Pro Lys Glu Ala Glu Arg Leu Tyr Arg Ser Gly Leu Ala Ser Leu
530 535 540
His Ala Arg Val Lys Val Arg Ile Thr Glu Tyr Glu Lys Asp Ala Asn
545 550 555 560
Gly Glu Leu Val Ala Lys Thr Ser Leu Lys Asp Thr Thr Val Gly Arg
565 570 575
Ala Ile Leu Trp Met Ile Val Pro Lys Gly Leu Pro Tyr Ser Ile Val
580 585 590
Asn Gln Ala Leu Gly Lys Lys Ala Ile Ser Lys Met Leu Asn Thr Cys
595 600 605
Tyr Arg Ile Leu Gly Leu Lys Pro Thr Val Ile Phe Ala Asp Gln Ile
610 615 620
Met Tyr Thr Gly Phe Ala Tyr Ala Ala Arg Ser Gly Ala Ser Val Gly
625 630 635 640
Ile Asp Asp Met Val Ile Pro Glu Lys Lys His Glu Ile Ile Ser Glu
645 650 655
Ala Glu Ala Glu Val Ala Glu Ile Gln Glu Gln Phe Gln Ser Gly Leu
660 665 670
Val Thr Ala Gly Glu Arg Tyr Asn Lys Val Ile Asp Ile Trp Ala Ala
675 680 685
Ala Asn Asp Arg Val Ser Lys Ala Met Met Asp Asn Leu Gln Thr Glu
690 695 700
Thr Val Ile Asn Arg Asp Gly Gln Glu Glu Lys Gln Val Ser Phe Asn
705 710 715 720
Ser Ile Tyr Met Met Ala Asp Ser Gly Ala Arg Gly Ser Ala Ala Gln
725 730 735
Ile Arg Gln Leu Ala Gly Met Arg Gly Leu Met Ala Lys Pro Asp Gly
740 745 750
Ser Ile Ile Glu Thr Pro Ile Thr Ala Asn Phe Arg Glu Gly Leu Asn
755 760 765
Val Leu Gln Tyr Phe Ile Ser Thr His Gly Ala Arg Lys Gly Leu Ala
770 775 780
Asp Thr Ala Leu Lys Thr Ala Asn Ser Gly Tyr Leu Thr Arg Arg Leu
785 790 795 800
Val Asp Val Ala Gln Asp Leu Val Val Thr Glu Asp Asp Cys Gly Thr
805 810 815
His Glu Gly Ile Met Met Thr Pro Val Ile Glu Gly Gly Asp Val Lys
820 825 830
Glu Pro Leu Arg Asp Arg Val Leu Gly Arg Val Thr Ala Glu Asp Val
835 840 845
Leu Lys Pro Gly Thr Ala Asp Ile Leu Val Pro Arg Asn Thr Leu Leu
850 855 860
His Glu Gln Trp Cys Asp Leu Leu Glu Glu Asn Ser Val Asp Ala Val
865 870 875 880
Lys Val Arg Ser Val Val Ser Cys Asp Thr Asp Phe Gly Val Cys Ala
885 890 895
His Cys Tyr Gly Arg Asp Leu Ala Arg Gly His Ile Ile Asn Lys Gly
900 905 910
Glu Ala Ile Gly Val Ile Ala Ala Gln Ser Ile Gly Glu Pro Gly Thr
915 920 925
Gln Leu Thr Met Arg Thr Phe His Ile Gly Gly Ala Ala Ser Arg Ala
930 935 940
Ala Ala Glu Ser Ser Ile Gln Val Lys Asn Lys Gly Ser Ile Lys Leu
945 950 955 960
Ser Asn Val Lys Ser Val Val Asn Ser Ser Gly Lys Leu Val Ile Thr
965 970 975
Ser Arg Asn Thr Glu Leu Lys Leu Ile Asp Glu Phe Gly Arg Thr Lys
980 985 990
Glu Ser Tyr Lys Val Pro Tyr Gly Ala Val Leu Ala Lys Gly Asp Gly
995 1000 1005
Glu Gln Val Ala Gly Gly Glu Thr Val Ala Asn Trp Asp Pro His Thr
1010 1015 1020
Met Pro Val Ile Thr Glu Val Ser Gly Phe Val Arg Phe Thr Asp Met
1025 1030 1035 1040
Ile Asp Gly Gln Thr Ile Thr Arg Gln Thr Asp Glu Leu Thr Gly Leu
1045 1050 1055
Ser Ser Leu Val Val Leu Asp Ser Ala Glu Arg Thr Ala Gly Gly Lys
1060 1065 1070
Asp Leu Arg Pro Ala Leu Lys Ile Val Asp Ala Gln Gly Asn Asp Val
1075 1080 1085
Leu Ile Pro Gly Thr Asp Met Pro Ala Gln Tyr Phe Leu Pro Gly Lys
1090 1095 1100
Ala Ile Val Gln Leu Glu Asp Gly Val Gln Ile Ser Ser Gly Asp Thr
1105 1110 1115 1120
Leu Ala Arg Ile Pro Gln Glu Ser Gly Gly Thr Lys Asp Ile Thr Gly
1125 1130 1135
Gly Leu Pro Arg Val Ala Asp Leu Phe Glu Ala Arg Arg Pro Lys Glu
1140 1145 1150
Pro Ala Ile Leu Ala Glu Ile Ser Gly Ile Val Ser Phe Gly Lys Glu
1155 1160 1165
Thr Lys Gly Lys Arg Arg Leu Val Ile Thr Pro Val Asp Gly Ser Asp
1170 1175 1180
Pro Tyr Glu Glu Met Ile Pro Lys Trp Arg Gln Leu Asn Val Phe Glu
1185 1190 1195 1200
Gly Glu Arg Val Glu Arg Gly Asp Val Ile Ser Asp Gly Pro Glu Ala
1205 1210 1215
Pro His Asp Ile Leu Arg Leu Arg Gly Val His Ala Val Thr Arg Tyr
1220 1225 1230
Ile Val Asn Glu Val Gln Asp Val Tyr Arg Leu Gln Gly Val Lys Ile
1235 1240 1245
Asn Asp Lys His Ile Glu Val Ile Val Arg Gln Met Leu Arg Lys Ala
1250 1255 1260
Thr Ile Val Asn Ala Gly Ser Ser Asp Phe Leu Glu Gly Glu Gln Val
1265 1270 1275 1280
Glu Tyr Ser Arg Val Lys Ile Ala Asn Arg Glu Leu Glu Ala Asn Gly
1285 1290 1295
Lys Val Gly Ala Thr Tyr Ser Arg Asp Leu Leu Gly Ile Thr Lys Ala
1300 1305 1310
Ser Leu Ala Thr Glu Ser Phe Ile Ser Ala Ala Ser Phe Gln Glu Thr
1315 1320 1325
Thr Arg Val Leu Thr Glu Ala Ala Val Ala Gly Lys Arg Asp Glu Leu
1330 1335 1340
Arg Gly Leu Lys Glu Asn Val Ile Val Gly Arg Leu Ile Pro Ala Gly
1345 1350 1355 1360
Thr Gly Tyr Ala Tyr His Gln Asp Arg Met Arg Arg Arg Ala Ala Gly
1365 1370 1375
Glu Ala Pro Ala Ala Pro Gln Val Thr Ala Glu Asp Ala Ser Ala Ser
1380 1385 1390
Leu Ala Glu Leu Leu Asn Ala Gly Leu Gly Gly Ser Asp Asn Glu
1395 1400 1405
<210> 101
<211> 1342
<212> PRT
<213> 福氏志贺氏菌
<400> 101
Met Val Tyr Ser Tyr Thr Glu Lys Lys Arg Ile Arg Lys Asp Phe Gly
1 5 10 15
Lys Arg Pro Gln Val Leu Asp Val Pro Tyr Leu Leu Ser Ile Gln Leu
20 25 30
Asp Ser Phe Gln Lys Phe Ile Glu Gln Asp Pro Glu Gly Gln Tyr Gly
35 40 45
Leu Glu Ala Ala Phe Arg Ser Val Phe Pro Ile Gln Ser Tyr Ser Gly
50 55 60
Asn Ser Glu Leu Gln Tyr Val Ser Tyr Arg Leu Gly Glu Pro Val Phe
65 70 75 80
Asp Val Gln Glu Cys Gln Ile Arg Gly Val Thr Tyr Ser Ala Pro Leu
85 90 95
Arg Val Lys Leu Arg Leu Val Ile Tyr Glu Arg Glu Ala Pro Glu Gly
100 105 110
Thr Val Lys Asp Ile Lys Glu Gln Glu Val Tyr Met Gly Glu Ile Pro
115 120 125
Leu Met Thr Asp Asn Gly Thr Phe Val Ile Asn Gly Thr Glu Arg Val
130 135 140
Ile Val Ser Gln Leu His Arg Ser Pro Gly Val Phe Phe Asp Ser Asp
145 150 155 160
Lys Gly Lys Thr His Ser Ser Gly Lys Val Leu Tyr Asn Ala Arg Ile
165 170 175
Ile Pro Tyr Arg Gly Ser Trp Leu Asp Phe Glu Phe Asp Pro Lys Asp
180 185 190
Asn Leu Phe Val Arg Ile Asp Arg Arg Arg Lys Leu Pro Ala Thr Ile
195 200 205
Ile Leu Arg Ala Leu Asn Tyr Thr Thr Glu Gln Ile Leu Asp Leu Phe
210 215 220
Phe Glu Lys Val Ile Phe Glu Ile Arg Asp Asn Lys Leu Gln Met Glu
225 230 235 240
Leu Val Pro Glu Arg Leu Arg Gly Glu Thr Ala Ser Phe Asp Ile Glu
245 250 255
Ala Asn Gly Lys Val Tyr Val Glu Lys Gly Arg Arg Ile Thr Ala Arg
260 265 270
His Ile Arg Gln Leu Glu Lys Asp Asp Val Lys Leu Ile Glu Val Pro
275 280 285
Val Glu Tyr Ile Ala Gly Lys Val Val Ala Lys Asp Tyr Ile Asp Glu
290 295 300
Ser Thr Gly Glu Leu Ile Cys Ala Ala Asn Met Glu Leu Ser Leu Asp
305 310 315 320
Leu Leu Ala Lys Leu Ser Gln Ser Gly His Lys Arg Ile Glu Thr Leu
325 330 335
Phe Thr Asn Asp Leu Asp His Gly Pro Tyr Ile Ser Glu Thr Leu Arg
340 345 350
Val Asp Pro Thr Asn Asp Arg Leu Ser Ala Leu Val Glu Ile Tyr Arg
355 360 365
Met Met Arg Pro Gly Glu Pro Pro Thr Arg Glu Ala Ala Glu Ser Leu
370 375 380
Phe Glu Asn Leu Phe Phe Ser Glu Asp Arg Tyr Asp Leu Ser Ala Val
385 390 395 400
Gly Arg Met Lys Phe Asn Arg Ser Leu Leu Arg Glu Glu Ile Glu Gly
405 410 415
Ser Gly Ile Leu Ser Lys Asp Asp Ile Ile Asp Val Met Lys Lys Leu
420 425 430
Ile Asp Ile Arg Asn Gly Lys Gly Glu Val Asp Asp Ile Asp His Leu
435 440 445
Gly Asn Arg Arg Ile Arg Ser Val Gly Glu Met Ala Glu Asn Gln Phe
450 455 460
Arg Val Gly Leu Val Arg Val Glu Arg Ala Val Lys Glu Arg Leu Ser
465 470 475 480
Leu Gly Asp Leu Asp Thr Leu Met Pro Gln Asp Met Ile Asn Ala Lys
485 490 495
Pro Ile Ser Ala Ala Val Lys Glu Phe Phe Gly Ser Ser Gln Leu Ser
500 505 510
Gln Phe Met Asp Gln Asn Asn Pro Leu Ser Glu Ile Thr His Lys Arg
515 520 525
Arg Ile Ser Ala Leu Gly Pro Gly Gly Leu Thr Arg Glu Arg Ala Gly
530 535 540
Phe Glu Val Arg Asp Val His Pro Thr His Tyr Gly Arg Val Cys Pro
545 550 555 560
Ile Glu Thr Pro Glu Gly Pro Asn Ile Gly Leu Ile Asn Ser Leu Ser
565 570 575
Val Tyr Ala Gln Thr Asn Glu Tyr Gly Phe Leu Glu Thr Pro Tyr Arg
580 585 590
Lys Val Thr Asp Gly Val Val Thr Asp Glu Ile His Tyr Leu Ser Ala
595 600 605
Ile Glu Glu Gly Asn Tyr Val Ile Ala Gln Ala Asn Ser Asn Leu Asp
610 615 620
Glu Glu Gly His Phe Val Glu Asp Leu Val Thr Cys Arg Ser Lys Gly
625 630 635 640
Glu Ser Ser Leu Phe Ser Arg Asp Gln Val Asp Tyr Met Asp Val Ser
645 650 655
Thr Gln Gln Val Val Ser Val Gly Ala Ser Leu Ile Pro Phe Leu Glu
660 665 670
His Asp Asp Ala Asn Arg Ala Leu Met Gly Ala Asn Met Gln Arg Gln
675 680 685
Ala Val Pro Thr Leu Arg Ala Asp Lys Pro Leu Val Gly Thr Gly Met
690 695 700
Glu Arg Ala Val Ala Val Asp Ser Gly Val Thr Ala Val Ala Lys Arg
705 710 715 720
Gly Gly Val Val Gln Tyr Val Asp Ala Ser Arg Ile Val Ile Lys Val
725 730 735
Asn Glu Asp Glu Met Tyr Pro Gly Glu Ala Gly Ile Asp Ile Tyr Asn
740 745 750
Leu Thr Lys Tyr Thr Arg Ser Asn Gln Asn Thr Cys Ile Asn Gln Met
755 760 765
Pro Cys Val Ser Leu Gly Glu Pro Val Glu Arg Gly Asp Val Leu Ala
770 775 780
Asp Gly Pro Ser Thr Asp Leu Gly Glu Leu Ala Leu Gly Gln Asn Met
785 790 795 800
Arg Val Ala Phe Met Pro Trp Asn Gly Tyr Asn Phe Glu Asp Ser Ile
805 810 815
Leu Val Ser Glu Arg Val Val Gln Glu Asp Arg Phe Thr Thr Ile His
820 825 830
Ile Gln Glu Leu Ala Cys Val Ser Arg Asp Thr Lys Leu Gly Pro Glu
835 840 845
Glu Ile Thr Ala Asp Ile Pro Asn Val Gly Glu Ala Ala Leu Ser Lys
850 855 860
Leu Asp Glu Ser Gly Ile Val Tyr Ile Gly Ala Glu Val Thr Gly Gly
865 870 875 880
Asp Ile Leu Val Gly Lys Val Thr Pro Lys Gly Glu Thr Gln Leu Thr
885 890 895
Pro Glu Glu Lys Leu Leu Arg Ala Ile Phe Gly Glu Lys Ala Ser Asp
900 905 910
Val Lys Asp Ser Ser Leu Arg Val Pro Asn Gly Val Ser Gly Thr Val
915 920 925
Ile Asp Val Gln Val Phe Thr Arg Asp Gly Val Glu Lys Asp Lys Arg
930 935 940
Ala Leu Glu Ile Glu Glu Met Gln Leu Lys Gln Ala Lys Lys Asp Leu
945 950 955 960
Ser Glu Glu Leu Gln Ile Leu Glu Ala Gly Leu Phe Ser Arg Ile Arg
965 970 975
Ala Val Leu Val Ala Gly Gly Val Glu Ala Glu Lys Leu Asp Lys Leu
980 985 990
Pro Arg Asp Arg Trp Leu Glu Leu Gly Leu Thr Asp Glu Glu Lys Gln
995 1000 1005
Asn Gln Leu Glu Gln Leu Ala Glu Gln Tyr Asp Glu Leu Lys His Glu
1010 1015 1020
Phe Glu Lys Lys Leu Glu Ala Lys Arg Arg Lys Ile Thr Gln Gly Asp
1025 1030 1035 1040
Asp Leu Ala Pro Gly Val Leu Lys Ile Val Lys Val Tyr Leu Ala Val
1045 1050 1055
Lys Arg Arg Ile Gln Pro Gly Asp Lys Met Ala Gly Arg His Gly Asn
1060 1065 1070
Lys Gly Val Ile Ser Lys Ile Asn Pro Ile Glu Asp Met Pro Tyr Asp
1075 1080 1085
Glu Asn Gly Thr Pro Val Asp Ile Val Leu Asn Pro Leu Gly Val Pro
1090 1095 1100
Ser Arg Met Asn Ile Gly Gln Ile Leu Glu Thr His Leu Gly Met Ala
1105 1110 1115 1120
Ala Lys Gly Ile Gly Asp Lys Ile Asn Ala Met Leu Lys Gln Gln Gln
1125 1130 1135
Glu Val Ala Lys Leu Arg Glu Phe Ile Gln Arg Ala Tyr Asp Leu Gly
1140 1145 1150
Ala Asp Val Arg Gln Lys Val Asp Leu Ser Thr Phe Ser Asp Glu Glu
1155 1160 1165
Val Met Arg Leu Ala Glu Asn Leu Arg Lys Gly Met Pro Ile Ala Thr
1170 1175 1180
Pro Val Phe Asp Gly Ala Lys Glu Ala Glu Ile Lys Glu Leu Leu Lys
1185 1190 1195 1200
Leu Gly Asp Leu Pro Thr Ser Gly Gln Ile Arg Leu Tyr Asp Gly Arg
1205 1210 1215
Thr Gly Glu Gln Phe Glu Arg Pro Val Thr Val Gly Tyr Met Tyr Met
1220 1225 1230
Leu Lys Leu Asn His Leu Val Asp Asp Lys Met His Ala Arg Ser Thr
1235 1240 1245
Gly Ser Tyr Ser Leu Val Thr Gln Gln Pro Leu Gly Gly Lys Ala Gln
1250 1255 1260
Phe Gly Gly Gln Arg Phe Gly Glu Met Glu Val Trp Ala Leu Glu Ala
1265 1270 1275 1280
Tyr Gly Ala Ala Tyr Thr Leu Gln Glu Met Leu Thr Val Lys Ser Asp
1285 1290 1295
Asp Val Asn Gly Arg Thr Lys Met Tyr Lys Asn Ile Val Asp Gly Asn
1300 1305 1310
His Gln Met Glu Pro Gly Met Pro Glu Ser Phe Asn Val Leu Leu Lys
1315 1320 1325
Glu Ile Arg Ser Leu Gly Ile Asn Ile Glu Leu Glu Asp Glu
1330 1335 1340
<210> 102
<211> 253
<212> PRT
<213> 福氏志贺氏菌
<400> 102
Met Ser Lys Ser Asp Val Phe His Leu Gly Leu Thr Lys Asn Asp Leu
1 5 10 15
Gln Gly Ala Thr Leu Ala Ile Val Pro Gly Asp Pro Asp Arg Val Glu
20 25 30
Lys Ile Ala Ala Leu Met Asp Lys Pro Val Lys Leu Ala Ser His Arg
35 40 45
Glu Phe Thr Thr Trp Arg Ala Glu Leu Asp Gly Lys Pro Val Ile Val
50 55 60
Cys Ser Thr Gly Ile Gly Gly Pro Ser Thr Ser Ile Ala Val Glu Glu
65 70 75 80
Leu Ala Gln Leu Gly Ile Arg Thr Phe Leu Arg Ile Gly Thr Thr Gly
85 90 95
Ala Ile Gln Pro His Ile Asn Val Gly Asp Val Leu Val Thr Thr Ala
100 105 110
Ser Val Arg Leu Asp Gly Ala Ser Leu His Phe Ala Pro Leu Glu Phe
115 120 125
Pro Ala Val Ala Asp Phe Glu Cys Thr Thr Ala Leu Val Glu Ala Ala
130 135 140
Lys Ser Ile Gly Ala Thr Thr His Val Gly Val Thr Val Ser Ser Asp
145 150 155 160
Thr Phe Tyr Pro Gly Gln Glu Arg Tyr Asp Thr Tyr Ser Gly Arg Val
165 170 175
Val Arg His Phe Lys Gly Ser Met Glu Glu Trp Gln Ala Met Gly Val
180 185 190
Met Asn Tyr Glu Met Glu Ser Ala Thr Leu Leu Thr Met Cys Ala Ser
195 200 205
Gln Gly Leu Arg Ala Gly Met Val Ala Gly Val Ile Val Asn Arg Thr
210 215 220
Gln Gln Glu Ile Pro Asn Ala Glu Thr Met Lys Gln Thr Glu Ser His
225 230 235 240
Ala Val Lys Ile Val Val Glu Ala Ala Arg Arg Leu Leu
245 250
<210> 103
<211> 289
<212> PRT
<213> 福氏志贺氏菌
<400> 103
Met Arg Thr Leu Gln Gly Trp Leu Leu Pro Val Phe Met Leu Pro Met
1 5 10 15
Ala Val Tyr Ala Gln Glu Ala Thr Val Lys Glu Val His Asp Ala Pro
20 25 30
Ala Val Arg Gly Ser Ile Ile Ala Asn Met Leu Gln Glu His Asp Asn
35 40 45
Pro Phe Thr Leu Tyr Pro Tyr Asp Thr Asn Tyr Leu Ile Tyr Thr Gln
50 55 60
Thr Ser Asp Leu Asn Lys Glu Ala Ile Ala Ser Tyr Asp Trp Ala Glu
65 70 75 80
Asn Ala Arg Lys Asp Glu Val Lys Phe Gln Leu Ser Leu Ala Phe Pro
85 90 95
Leu Trp Arg Gly Ile Leu Gly Pro Asn Ser Val Leu Gly Ala Ser Tyr
100 105 110
Thr Gln Lys Ser Trp Trp Gln Leu Ser Asn Ser Glu Glu Ser Ser Pro
115 120 125
Phe Arg Glu Thr Asn Tyr Glu Pro Gln Leu Phe Leu Gly Phe Ala Thr
130 135 140
Asp Tyr Arg Phe Ala Gly Trp Thr Leu Arg Asp Val Glu Met Gly Tyr
145 150 155 160
Asn His Asp Ser Asn Gly Arg Ser Asp Pro Thr Ser Arg Ser Trp Asn
165 170 175
Arg Leu Tyr Thr Arg Leu Met Ala Glu Asn Gly Asn Trp Leu Val Glu
180 185 190
Val Lys Pro Trp Tyr Val Val Gly Asn Thr Asp Asp Asn Pro Asp Ile
195 200 205
Thr Lys Tyr Met Gly Tyr Tyr Gln Leu Lys Ile Gly Tyr His Leu Gly
210 215 220
Asp Ala Val Leu Ser Ala Lys Gly Gln Tyr Asn Trp Asn Thr Gly Tyr
225 230 235 240
Gly Gly Ala Glu Leu Gly Leu Ser Tyr Pro Ile Thr Lys His Val Arg
245 250 255
Leu Tyr Thr Gln Val Tyr Ser Gly Tyr Gly Glu Ser Leu Ile Asp Tyr
260 265 270
Asn Phe Asn Gln Thr Arg Val Gly Val Gly Val Met Leu Asn Asp Leu
275 280 285
Phe
<210> 104
<211> 460
<212> PRT
<213> 福氏志贺氏菌
<400> 104
Met Ala Thr Gly Lys Ile Val Gln Val Ile Gly Ala Val Val Asp Val
1 5 10 15
Glu Phe Pro Gln Asp Ala Val Pro Arg Val Tyr Asp Ala Leu Glu Val
20 25 30
Gln Asn Gly Asn Glu Arg Leu Val Leu Glu Val Gln Gln Gln Leu Gly
35 40 45
Gly Gly Ile Val Arg Thr Ile Ala Met Gly Ser Ser Asp Gly Leu Arg
50 55 60
Arg Gly Leu Asp Val Lys Asp Leu Glu His Pro Ile Glu Val Pro Val
65 70 75 80
Gly Lys Ala Thr Leu Gly Arg Ile Met Asn Val Leu Gly Glu Pro Val
85 90 95
Asp Met Lys Gly Glu Ile Gly Glu Glu Glu Arg Trp Ala Ile His Arg
100 105 110
Ala Ala Pro Ser Tyr Glu Glu Leu Ser Asn Ser Gln Glu Leu Leu Glu
115 120 125
Thr Gly Ile Lys Val Ile Asp Leu Met Cys Pro Phe Ala Lys Gly Gly
130 135 140
Lys Val Gly Leu Phe Gly Gly Ala Gly Val Gly Lys Thr Val Asn Met
145 150 155 160
Met Glu Leu Ile Arg Asn Ile Ala Ile Glu His Ser Gly Tyr Ser Val
165 170 175
Phe Ala Gly Val Gly Glu Arg Thr Arg Glu Gly Asn Asp Phe Tyr His
180 185 190
Glu Met Thr Asp Ser Asn Val Ile Asp Lys Val Ser Leu Val Tyr Gly
195 200 205
Gln Met Asn Glu Pro Pro Gly Asn Arg Leu Arg Val Ala Leu Thr Gly
210 215 220
Leu Thr Met Ala Glu Lys Phe Arg Asp Glu Gly Arg Asp Val Leu Leu
225 230 235 240
Phe Val Asp Asn Ile Tyr Arg Tyr Thr Leu Ala Gly Thr Glu Val Ser
245 250 255
Ala Leu Leu Gly Arg Met Pro Ser Ala Val Gly Tyr Gln Pro Thr Leu
260 265 270
Ala Glu Glu Met Gly Val Leu Gln Glu Arg Ile Thr Ser Thr Lys Thr
275 280 285
Gly Ser Ile Thr Ser Val Gln Ala Val Tyr Val Pro Ala Asp Asp Leu
290 295 300
Thr Asp Pro Ser Pro Ala Thr Thr Phe Ala His Leu Asp Ala Thr Val
305 310 315 320
Val Leu Ser Arg Gln Ile Ala Ser Leu Gly Ile Tyr Pro Ala Val Asp
325 330 335
Pro Leu Asp Ser Thr Ser Arg Gln Leu Asp Pro Leu Val Val Gly Gln
340 345 350
Glu His Tyr Asp Thr Ala Arg Gly Val Gln Ser Ile Leu Gln Arg Tyr
355 360 365
Gln Glu Leu Lys Asp Ile Ile Ala Ile Leu Gly Met Asp Glu Leu Ser
370 375 380
Glu Glu Asp Lys Leu Val Val Ala Arg Ala Arg Lys Ile Gln Arg Phe
385 390 395 400
Leu Ser Gln Pro Phe Phe Val Ala Glu Val Phe Thr Gly Ser Pro Gly
405 410 415
Lys Tyr Val Ser Leu Lys Asp Thr Ile Arg Gly Phe Lys Gly Ile Met
420 425 430
Glu Gly Glu Tyr Asp His Leu Pro Glu Gln Ala Phe Tyr Met Val Gly
435 440 445
Ser Ile Glu Glu Ala Val Glu Lys Ala Lys Lys Leu
450 455 460
<210> 105
<211> 731
<212> PRT
<213> 福氏志贺氏菌
<400> 105
Met Arg Lys Lys Tyr Met Pro Arg Ala Leu Gly Pro Leu Leu Leu Val
1 5 10 15
Val Leu Ser Pro Ala Val Ala Gln Gln Asn Asp Asp Asn Glu Ile Ile
20 25 30
Val Ser Ala Ser Arg Ser Asn Arg Thr Val Ala Glu Met Ala Gln Thr
35 40 45
Thr Trp Val Ile Glu Asn Ala Glu Leu Glu Gln Gln Ile Gln Gly Gly
50 55 60
Lys Glu Leu Lys Asp Ala Leu Ala Gln Leu Ile Pro Gly Leu Asp Val
65 70 75 80
Ser Ser Gln Ser Arg Thr Asn Tyr Gly Met Asn Met Arg Gly Arg Pro
85 90 95
Leu Val Val Leu Ile Asp Gly Val Arg Leu Asn Ser Ser Arg Ser Asp
100 105 110
Ser Arg Gln Leu Asp Ser Val Asp Pro Phe Asn Ile Asp His Ile Glu
115 120 125
Val Ile Ser Gly Ala Thr Ala Leu Tyr Gly Gly Gly Ser Thr Gly Gly
130 135 140
Leu Ile Asn Ile Val Thr Lys Lys Gly Gln Pro Glu Thr Met Met Glu
145 150 155 160
Phe Glu Ala Gly Thr Lys Ser Gly Phe Asn Ser Ser Lys Asp His Asp
165 170 175
Glu Arg Ile Ala Gly Ala Val Ser Gly Gly Asn Asp His Ile Ser Gly
180 185 190
Arg Leu Ser Val Ala Tyr Gln Lys Phe Gly Gly Trp Phe Asp Gly Asn
195 200 205
Gly Asp Ala Thr Leu Leu Asp Asn Thr Gln Thr Gly Leu Gln His Ser
210 215 220
Asn Arg Leu Asp Ile Met Gly Thr Gly Thr Leu Asn Ile Asp Glu Ser
225 230 235 240
Arg Gln Leu Gln Leu Ile Thr Gln Tyr Tyr Lys Ser Gln Gly Asp Asp
245 250 255
Asn Tyr Gly Leu Asn Leu Gly Lys Gly Phe Ser Ala Ile Ser Gly Ser
260 265 270
Ser Thr Pro Tyr Val Ser Lys Gly Leu Asn Ser Asp Arg Ile Pro Gly
275 280 285
Thr Glu Arg His Leu Ile Ser Leu Gln Tyr Ser Asp Ser Asp Phe Leu
290 295 300
Gly Gln Glu Leu Val Gly Gln Val Tyr Tyr Arg Asp Glu Ser Leu Arg
305 310 315 320
Tyr Tyr Pro Phe Pro Thr Val Asn Ala Asn Lys Gln Ala Thr Ala Phe
325 330 335
Ser Ser Ser Gln Gln Asp Thr Asp Gln Tyr Gly Met Lys Leu Thr Leu
340 345 350
Asn Ser Gln Leu Met Asp Gly Trp Gln Ile Thr Trp Gly Leu Asp Ala
355 360 365
Glu His Glu Arg Phe Thr Ser Asn Gln Met Phe Phe Asp Leu Ala Gln
370 375 380
Ala Ser Ala Ser Gly Gly Leu Asn Asn His Lys Ile Tyr Thr Thr Gly
385 390 395 400
Arg Tyr Pro Ser Tyr Asp Ile Thr Asn Leu Ala Ala Phe Leu Gln Ser
405 410 415
Ser Tyr Asp Ile Asn Asp Ile Phe Thr Val Ser Gly Gly Val Arg Tyr
420 425 430
Gln Tyr Thr Glu Asn Arg Val Asp Asp Phe Ile Asp Tyr Thr Gln Gln
435 440 445
Gln Lys Ile Ala Thr Gly Lys Ala Ile Ser Ala Asp Ala Ile Pro Gly
450 455 460
Gly Ser Val Asp Tyr Asp Asn Phe Leu Phe Asn Ala Gly Leu Leu Met
465 470 475 480
His Ile Thr Glu Arg Gln Gln Ala Trp Phe Asn Phe Ser Gln Gly Val
485 490 495
Ala Leu Pro Asp Pro Gly Lys Tyr Tyr Gly Arg Gly Ile Tyr Gly Ala
500 505 510
Ala Val Asn Gly His Leu Pro Leu Thr Lys Ser Val Asn Val Ser Asp
515 520 525
Ser Lys Leu Glu Gly Val Lys Val Asp Ser Tyr Glu Leu Gly Trp Arg
530 535 540
Phe Ile Gly Asp Asn Leu Arg Thr Gln Ile Ala Ala Tyr Tyr Ser Leu
545 550 555 560
Ser Asn Lys Ser Val Glu Arg Asn Lys Asp Leu Thr Ile Ser Val Lys
565 570 575
Asp Asp Arg Arg Arg Ile Tyr Gly Val Glu Gly Ala Val Asp Tyr Leu
580 585 590
Ile Pro Asp Thr Asp Trp Ser Thr Gly Val Asn Phe Asn Val Leu Lys
595 600 605
Thr Glu Ser Lys Val Asn Gly Gln Trp Gln Lys Tyr Asp Val Lys Glu
610 615 620
Ser Ser Pro Ser Lys Ala Thr Ala Tyr Ile Asn Trp Ala Pro Glu Pro
625 630 635 640
Trp Ser Leu Arg Val Gln Ser Thr Thr Ser Phe Asp Val Ser Asp Ala
645 650 655
Glu Gly Asn Asp Ile Asn Gly Tyr Thr Thr Val Asp Phe Ile Ser Ser
660 665 670
Trp Gln Leu Pro Val Gly Thr Leu Ser Phe Ser Val Glu Asn Leu Phe
675 680 685
Asp Arg Asp Tyr Thr Thr Val Trp Gly Gln Arg Ala Pro Leu Tyr Tyr
690 695 700
Ser Pro Gly Tyr Gly Pro Ala Ser Leu Tyr Asp Tyr Lys Gly Arg Gly
705 710 715 720
Arg Thr Phe Gly Leu Asn Tyr Ser Val Leu Phe
725 730
<210> 106
<211> 396
<212> PRT
<213> 福氏志贺氏菌
<400> 106
Met Ile Ile Ser Ala Ala Ser Asp Tyr Arg Ala Ala Ala Gln Arg Ile
1 5 10 15
Leu Pro Pro Phe Leu Phe His Tyr Met Asp Gly Gly Ala Tyr Ser Glu
20 25 30
Tyr Thr Leu Arg Arg Asn Val Glu Asp Leu Ser Glu Val Ala Leu Arg
35 40 45
Gln Arg Ile Leu Lys Asn Met Ser Asp Leu Ser Leu Glu Thr Thr Leu
50 55 60
Phe Asn Glu Lys Leu Ser Met Pro Val Ala Leu Ala Pro Val Gly Leu
65 70 75 80
Cys Gly Met Tyr Ala Arg Arg Gly Glu Val Gln Ala Ala Lys Ala Ala
85 90 95
Asp Ala His Gly Ile Pro Phe Thr Leu Ser Thr Val Ser Val Cys Pro
100 105 110
Ile Glu Glu Val Ala Pro Ala Ile Lys Arg Pro Met Trp Phe Gln Leu
115 120 125
Tyr Val Leu Arg Asp Arg Gly Phe Met Arg Asn Ala Leu Glu Arg Ala
130 135 140
Lys Ala Ala Gly Cys Ser Thr Leu Val Phe Thr Val Asp Met Pro Thr
145 150 155 160
Pro Gly Ala Arg Tyr Arg Asp Ala His Ser Gly Met Ser Gly Pro Asn
165 170 175
Ala Ala Met Arg Arg Tyr Leu Gln Ala Val Thr His Pro Gln Trp Ala
180 185 190
Trp Asp Val Gly Leu Asn Gly Arg Pro His Asp Leu Gly Asn Ile Ser
195 200 205
Ala Tyr Leu Gly Lys Pro Thr Gly Leu Glu Asp Tyr Ile Gly Trp Leu
210 215 220
Gly Asn Asn Phe Asp Pro Ser Ile Ser Trp Lys Asp Leu Glu Trp Ile
225 230 235 240
Arg Asp Phe Trp Asp Gly Pro Met Val Ile Lys Gly Ile Leu Asp Pro
245 250 255
Glu Asp Ala Arg Asp Ala Val Arg Phe Gly Ala Asp Gly Ile Val Val
260 265 270
Ser Asn His Gly Gly Arg Gln Leu Asp Gly Val Leu Ser Ser Ala Arg
275 280 285
Ala Leu Pro Ala Ile Ala Asp Ala Val Lys Gly Asp Ile Ala Ile Leu
290 295 300
Ala Asp Ser Gly Ile Arg Asn Gly Leu Asp Val Val Arg Met Ile Ala
305 310 315 320
Leu Gly Ala Asp Thr Val Leu Leu Gly Arg Ala Phe Leu Tyr Ala Leu
325 330 335
Ala Thr Ala Gly Gln Ala Gly Val Ala Asn Leu Leu Asn Leu Ile Glu
340 345 350
Lys Glu Met Lys Val Ala Met Thr Leu Thr Gly Ala Lys Ser Ile Ser
355 360 365
Glu Ile Thr Gln Asp Ser Leu Val Gln Gly Leu Gly Lys Glu Leu Pro
370 375 380
Thr Ala Leu Ala Pro Met Ala Lys Gly Asn Ala Ala
385 390 395
<210> 107
<211> 524
<212> PRT
<213> 福氏志贺氏菌
<400> 107
Met Leu Ser Thr Leu Arg Arg Thr Leu Phe Ala Leu Leu Ala Cys Ala
1 5 10 15
Ser Phe Ile Val His Ala Ala Ala Pro Asp Glu Ile Thr Thr Ala Trp
20 25 30
Pro Val Asn Val Gly Pro Leu Asn Pro His Leu Tyr Thr Pro Asn Gln
35 40 45
Met Phe Ala Gln Ser Met Val Tyr Glu Pro Leu Val Lys Tyr Gln Ala
50 55 60
Asp Gly Ser Val Ile Pro Trp Leu Ala Lys Ser Trp Thr His Ser Glu
65 70 75 80
Asp Gly Lys Thr Trp Thr Phe Thr Leu Arg Asp Asp Val Lys Phe Ser
85 90 95
Asn Gly Glu Pro Phe Asp Ala Glu Ala Ala Ala Glu Asn Phe Arg Ala
100 105 110
Val Leu Asp Asn Arg Gln Arg His Ala Trp Leu Glu Leu Ala Asn Gln
115 120 125
Ile Val Asp Val Lys Ala Leu Asn Lys Thr Glu Leu Gln Ile Thr Leu
130 135 140
Lys Ser Ala Tyr Tyr Pro Phe Leu Gln Glu Leu Ala Leu Pro Arg Pro
145 150 155 160
Phe Arg Phe Ile Ala Pro Ser Gln Phe Lys Asn His Glu Thr Met Asn
165 170 175
Gly Ile Lys Ala Pro Ile Gly Thr Gly Pro Trp Ile Leu Gln Glu Ser
180 185 190
Lys Leu Asn Gln Tyr Asp Val Phe Val Arg Asn Glu Asn Tyr Trp Gly
195 200 205
Glu Lys Pro Ala Ile Lys Lys Ile Thr Phe Asn Val Ile Pro Asp Pro
210 215 220
Thr Thr Arg Ala Val Ala Phe Glu Thr Gly Asp Ile Asp Leu Leu Tyr
225 230 235 240
Gly Asn Glu Gly Leu Leu Pro Leu Asp Thr Phe Ala Arg Phe Ser Gln
245 250 255
Asn Pro Ala Tyr His Thr Gln Leu Ser Gln Pro Ile Glu Thr Val Met
260 265 270
Leu Ala Leu Asn Thr Ala Lys Ala Pro Thr Asn Glu Leu Ala Val Arg
275 280 285
Glu Ala Leu Asn Tyr Ala Val Asn Lys Lys Ser Leu Ile Asp Asn Ala
290 295 300
Leu Tyr Gly Thr Gln Gln Val Ala Asp Thr Leu Phe Ala Pro Ser Val
305 310 315 320
Pro Tyr Ala Asn Leu Gly Leu Lys Pro Arg Gln Tyr Asp Pro Gln Lys
325 330 335
Ala Lys Glu Leu Leu Glu Lys Ala Gly Trp Thr Leu Pro Ala Gly Lys
340 345 350
Asp Ile Arg Glu Lys Asn Gly Gln Pro Leu Arg Ile Glu Leu Ser Phe
355 360 365
Ile Gly Thr Asp Ala Leu Ser Lys Ser Met Ala Glu Ile Ile Gln Ala
370 375 380
Asp Met Arg Gln Ile Gly Ala Asp Val Ser Leu Ile Gly Glu Glu Glu
385 390 395 400
Ser Ser Ile Tyr Ala Arg Gln Arg Asp Gly Arg Phe Gly Met Ile Phe
405 410 415
His Arg Thr Trp Gly Ala Pro Tyr Asp Pro His Ala Phe Leu Ser Ser
420 425 430
Met Arg Val Pro Ser His Ala Asp Phe Gln Ala Gln Gln Gly Leu Ala
435 440 445
Asp Lys Pro Leu Ile Asp Lys Glu Ile Gly Glu Val Leu Ala Thr His
450 455 460
Asp Glu Thr Gln Arg Gln Ala Leu Tyr Arg Asp Ile Leu Thr Arg Leu
465 470 475 480
His Asp Glu Ala Val Tyr Leu Pro Ile Ser Tyr Ile Ser Met Met Val
485 490 495
Val Ser Lys Pro Glu Leu Gly Asn Ile Pro Tyr Ala Pro Ile Ala Thr
500 505 510
Glu Ile Pro Phe Glu Gln Ile Lys Pro Val Lys Pro
515 520
<210> 108
<211> 492
<212> PRT
<213> 福氏志贺氏菌
<400> 108
Met Ala Ala Ser Met Val Leu Val Ser Arg Ala Asp Ala Ala Gly Arg
1 5 10 15
Gly Leu Ser Val Leu Met Leu Glu Ala Gln Asp Leu Ala Cys Ala Thr
20 25 30
Ser Ser Ala Ser Ser Lys Leu Ile His Gly Gly Leu Arg Tyr Leu Glu
35 40 45
His Tyr Glu Phe Arg Leu Val Ser Glu Ala Leu Ala Glu Arg Glu Val
50 55 60
Leu Leu Lys Met Ala Pro His Ile Ala Phe Pro Met Arg Phe Arg Leu
65 70 75 80
Pro His Arg Pro His Leu Arg Pro Ala Trp Met Ile Arg Ile Gly Leu
85 90 95
Phe Met Tyr Asp His Leu Gly Lys Arg Thr Ser Leu Pro Gly Ser Thr
100 105 110
Gly Leu Arg Phe Gly Ala Asn Ser Val Leu Lys Pro Glu Ile Lys Arg
115 120 125
Gly Phe Glu Tyr Ser Asp Cys Trp Val Asp Asp Ala Arg Leu Val Leu
130 135 140
Ala Asn Ala Gln Met Val Val Arg Lys Gly Gly Glu Val Leu Thr Arg
145 150 155 160
Thr Arg Ala Thr Ser Ala Arg Arg Glu Asn Gly Leu Trp Ile Val Glu
165 170 175
Ala Glu Asp Ile Asp Thr Gly Lys Lys Tyr Ser Trp Gln Ala Arg Gly
180 185 190
Leu Val Asn Ala Thr Gly Pro Trp Val Lys Gln Phe Phe Asp Glu Gly
195 200 205
Met His Leu Pro Ser Pro Tyr Gly Ile Arg Leu Ile Lys Gly Ser His
210 215 220
Ile Val Val Pro Arg Val His Thr Gln Lys Gln Ala Tyr Ile Leu Gln
225 230 235 240
Asn Glu Asp Lys Arg Ile Val Phe Val Ile Pro Trp Met Asp Glu Phe
245 250 255
Ser Ile Ile Gly Thr Thr Asp Val Glu Tyr Lys Gly Asp Pro Lys Ala
260 265 270
Val Lys Ile Glu Glu Ser Glu Ile Asn Tyr Leu Leu Lys Val Tyr Asn
275 280 285
Ala His Phe Lys Lys Gln Leu Ser Arg Asp Asp Ile Val Trp Thr Tyr
290 295 300
Ser Gly Val Arg Pro Leu Cys Asp Asp Glu Ser Asp Ser Pro Gln Ala
305 310 315 320
Ile Thr Arg Asp Tyr Thr Leu Asp Ile His Asp Glu Asn Gly Lys Ala
325 330 335
Pro Leu Leu Ser Val Phe Gly Gly Lys Leu Thr Thr Tyr Arg Lys Leu
340 345 350
Ala Glu His Ala Leu Glu Lys Leu Thr Pro Tyr Tyr Gln Gly Ile Gly
355 360 365
Pro Ala Trp Thr Lys Glu Ser Val Leu Pro Gly Gly Ala Ile Glu Gly
370 375 380
Asp Arg Asp Asp Tyr Ala Ala Arg Leu Arg Arg Arg Tyr Pro Phe Leu
385 390 395 400
Thr Glu Ser Leu Ala Arg His Tyr Thr Arg Thr Tyr Gly Ser Asn Ser
405 410 415
Glu Leu Leu Leu Gly Asn Ala Gly Thr Val Ser Asp Leu Gly Glu Asp
420 425 430
Phe Gly His Glu Phe Tyr Glu Ala Glu Leu Lys Tyr Leu Val Asp His
435 440 445
Glu Trp Val Arg Arg Thr Asp Asp Ala Leu Trp Arg Arg Thr Lys Gln
450 455 460
Gly Met Trp Leu Asn Ala Asp Gln Gln Ser Arg Val Ser Gln Trp Leu
465 470 475 480
Val Glu Tyr Thr Gln Gln Lys Leu Ser Leu Ala Ser
485 490
<210> 109
<211> 329
<212> PRT
<213> 福氏志贺氏菌
<400> 109
Met Gln Gly Ser Val Thr Glu Phe Leu Lys Pro Arg Leu Val Asp Ile
1 5 10 15
Glu Gln Val Ser Ser Thr His Ala Lys Val Thr Leu Glu Pro Leu Glu
20 25 30
Arg Gly Phe Gly His Thr Leu Gly Asn Ala Leu Arg Arg Ile Leu Leu
35 40 45
Ser Ser Met Pro Gly Cys Ala Val Thr Glu Val Glu Ile Asp Gly Val
50 55 60
Leu His Glu Tyr Ser Thr Lys Glu Gly Val Gln Glu Asp Ile Leu Glu
65 70 75 80
Ile Leu Leu Asn Leu Lys Gly Leu Ala Val Arg Val Gln Gly Lys Asp
85 90 95
Glu Val Ile Leu Thr Leu Asn Lys Ser Gly Ile Gly Pro Val Thr Ala
100 105 110
Ala Asp Ile Thr His Asp Gly Asp Val Glu Ile Val Lys Pro Gln His
115 120 125
Val Ile Cys His Leu Thr Asp Glu Asn Ala Ser Ile Ser Met Arg Ile
130 135 140
Lys Val Gln Arg Gly Arg Gly Tyr Val Pro Ala Ser Thr Arg Ile His
145 150 155 160
Ser Glu Glu Asp Glu Arg Pro Ile Gly Arg Leu Leu Val Asp Ala Cys
165 170 175
Tyr Ser Pro Val Glu Arg Ile Ala Tyr Asn Val Glu Ala Ala Arg Val
180 185 190
Glu Gln Arg Thr Asp Leu Asp Lys Leu Val Ile Glu Met Glu Thr Asn
195 200 205
Gly Thr Ile Asp Pro Glu Glu Ala Ile Arg Arg Ala Ala Thr Ile Leu
210 215 220
Ala Glu Gln Leu Glu Ala Phe Val Asp Leu Arg Asp Val Arg Gln Pro
225 230 235 240
Glu Val Lys Glu Glu Lys Pro Glu Phe Asp Pro Ile Leu Leu Arg Pro
245 250 255
Val Asp Asp Leu Glu Leu Thr Val Arg Ser Ala Asn Cys Leu Lys Ala
260 265 270
Glu Ala Ile His Tyr Ile Gly Asp Leu Val Gln Arg Thr Glu Val Glu
275 280 285
Leu Leu Lys Thr Pro Asn Leu Gly Lys Lys Ser Leu Thr Glu Ile Lys
290 295 300
Asp Val Leu Ala Ser Arg Gly Leu Ser Leu Gly Met Arg Leu Glu Asn
305 310 315 320
Trp Pro Pro Ala Ser Ile Ala Asp Glu
325
<210> 110
<211> 102
<212> PRT
<213> 福氏志贺氏菌
<400> 110
Met Ala Lys Gly Gln Ser Leu Gln Asp Pro Phe Leu Asn Ala Leu Arg
1 5 10 15
Arg Glu Arg Val Pro Val Ser Ile Tyr Leu Val Asn Gly Ile Lys Leu
20 25 30
Gln Gly Gln Ile Glu Ser Phe Asp Gln Phe Val Ile Leu Leu Lys Asn
35 40 45
Thr Val Ser Gln Met Val Tyr Lys His Ala Ile Ser Thr Val Val Pro
50 55 60
Ser Arg Pro Val Ser His His Ser Asn Asn Ala Gly Gly Gly Thr Ser
65 70 75 80
Ser Asn Tyr His His Gly Ser Ser Ala Gln Asn Thr Ser Ala Gln Gln
85 90 95
Asp Ser Glu Glu Thr Glu
100
<210> 111
<211> 379
<212> PRT
<213> 福氏志贺氏菌
<400> 111
Met Lys Lys Asn Ile Phe Lys Phe Ser Val Leu Thr Leu Ala Val Leu
1 5 10 15
Ser Leu Thr Ala Cys Thr Leu Val Pro Gly Gln Asn Leu Ser Thr Ser
20 25 30
Asn Lys Asp Val Ile Glu Leu Pro Asp Asn Gln Tyr Asp Leu Asp Lys
35 40 45
Met Val Asn Ile Tyr Pro Val Thr Pro Gly Leu Ile Asp Gln Leu Arg
50 55 60
Ala Lys Pro Ile Met Ser Gln Ala Asn Pro Glu Leu Glu Gln Gln Ile
65 70 75 80
Ala Asn Tyr Glu Tyr Arg Ile Gly Ile Gly Asp Val Leu Met Val Thr
85 90 95
Val Trp Asp His Pro Glu Leu Thr Thr Pro Ala Gly Gln Tyr Arg Ser
100 105 110
Ala Ser Asp Thr Gly Asn Trp Val Asn Ala Asp Gly Ala Ile Phe Tyr
115 120 125
Pro Tyr Ile Gly Arg Leu Lys Val Ala Gly Lys Thr Leu Thr Gln Val
130 135 140
Arg Asn Glu Ile Thr Ala Arg Leu Asp Ser Val Ile Glu Ser Pro Gln
145 150 155 160
Val Asp Val Ser Val Ala Ala Phe Arg Ser Gln Lys Ala Tyr Val Thr
165 170 175
Gly Glu Val Ser Lys Ser Gly Gln Gln Pro Ile Thr Asn Ile Pro Leu
180 185 190
Thr Ile Met Asp Ala Ile Asn Ala Ala Gly Gly Leu Thr Ala Asp Ala
195 200 205
Asp Trp Arg Asn Val Val Leu Thr Gln Asn Gly Val Lys Thr Lys Val
210 215 220
Asn Leu Tyr Ala Leu Met Gln Arg Gly Asp Leu Arg Gln Asn Lys Leu
225 230 235 240
Leu His Pro Gly Asp Ile Leu Phe Ile Pro Arg Asn Asp Asp Leu Lys
245 250 255
Val Phe Val Met Gly Glu Val Gly Lys Gln Ser Thr Leu Lys Met Asp
260 265 270
Arg Ser Gly Met Thr Leu Ala Glu Ala Leu Gly Asn Ala Glu Gly Met
275 280 285
Asn Gln Asp Val Ala Asp Ala Thr Gly Ile Phe Val Ile Arg Ala Thr
290 295 300
Gln Asn Lys Gln Asn Gly Lys Ile Ala Asn Ile Tyr Gln Leu Asn Ala
305 310 315 320
Lys Asp Ala Ser Ala Met Ile Leu Gly Thr Glu Phe Gln Leu Glu Pro
325 330 335
Tyr Asp Ile Val Tyr Val Thr Thr Ala Pro Leu Ala Arg Trp Asn Arg
340 345 350
Val Ile Ser Leu Leu Val Pro Thr Ile Ser Gly Val His Asp Leu Thr
355 360 365
Glu Thr Ser Arg Trp Ile Gln Thr Trp Pro Asn
370 375
<210> 112
<211> 445
<212> PRT
<213> 福氏志贺氏菌
<400> 112
Met Ile Thr Leu Arg Lys Leu Pro Leu Ala Val Ala Val Ala Ala Gly
1 5 10 15
Val Met Ser Ala Gln Ala Met Ala Val Asp Phe His Gly Tyr Ala Arg
20 25 30
Ser Gly Ile Gly Trp Thr Gly Ser Gly Gly Glu Gln Gln Cys Phe Gln
35 40 45
Thr Thr Gly Ala Gln Ser Lys Tyr Arg Leu Gly Asn Glu Cys Glu Thr
50 55 60
Tyr Ala Glu Leu Lys Leu Gly Gln Glu Val Trp Lys Glu Gly Asp Lys
65 70 75 80
Ser Phe Tyr Phe Asp Thr Asn Val Ala Tyr Ser Val Ala Gln Gln Asn
85 90 95
Asp Trp Glu Ala Thr Asp Pro Ala Phe Arg Glu Ala Asn Val Gln Gly
100 105 110
Lys Asn Leu Ile Glu Trp Leu Pro Gly Ser Thr Ile Trp Ala Gly Lys
115 120 125
Arg Phe Tyr Gln Arg His Asp Val His Met Ile Asp Phe Tyr Tyr Trp
130 135 140
Asp Ile Ser Gly Pro Gly Ala Gly Leu Glu Asn Ile Asp Val Gly Phe
145 150 155 160
Gly Lys Leu Ser Leu Ala Ala Thr Arg Ser Ser Glu Ala Gly Gly Ser
165 170 175
Ser Ser Phe Ala Ser Asn Asn Ile Tyr Asp Tyr Thr Asn Glu Thr Ala
180 185 190
Asn Asp Val Phe Asp Val Arg Leu Ala Gln Met Glu Ile Asn Pro Gly
195 200 205
Gly Thr Leu Glu Leu Gly Val Asp Tyr Gly Arg Ala Asn Leu Arg Asp
210 215 220
Asn Tyr Arg Leu Val Asp Gly Ala Ser Lys Asp Gly Trp Leu Phe Thr
225 230 235 240
Ala Glu His Thr Gln Ser Val Leu Lys Gly Phe Asn Lys Phe Val Val
245 250 255
Gln Tyr Ala Thr Asp Ser Met Thr Ser Gln Gly Lys Gly Leu Ser Gln
260 265 270
Gly Ser Gly Val Ala Phe Asp Asn Glu Lys Phe Ala Tyr Asn Ile Asn
275 280 285
Asn Asn Gly His Met Leu Arg Ile Leu Asp His Gly Ala Ile Ser Met
290 295 300
Gly Asp Asn Trp Asp Met Met Tyr Val Gly Met Tyr Gln Asp Ile Asn
305 310 315 320
Trp Asp Asn Asp Asn Gly Thr Lys Trp Trp Thr Val Gly Ile Arg Pro
325 330 335
Met Tyr Lys Trp Thr Pro Ile Met Ser Thr Val Met Glu Ile Gly Tyr
340 345 350
Asp Asn Val Glu Ser Gln Arg Thr Gly Asp Lys Asn Asn Gln Tyr Lys
355 360 365
Ile Thr Leu Ala Gln Gln Trp Gln Ala Gly Asp Ser Ile Trp Ser Arg
370 375 380
Pro Ala Ile Arg Val Phe Ala Thr Tyr Ala Lys Trp Asp Glu Lys Trp
385 390 395 400
Gly Tyr Asp Tyr Asn Gly Asp Ser Lys Val Asn Pro Asn Tyr Gly Lys
405 410 415
Ala Val Pro Ala Asp Phe Asn Gly Gly Ser Phe Gly Arg Gly Asp Ser
420 425 430
Asp Glu Trp Thr Phe Gly Ala Gln Met Glu Ile Trp Trp
435 440 445
<210> 113
<211> 1262
<212> PRT
<213> 福氏志贺氏菌
<400> 113
Met Ala Asp Phe Ser Ser Ala Thr Ile Thr Thr Gly Ser Leu Pro Pro
1 5 10 15
Gly Asp His Thr Leu Tyr Ser Pro Gln Tyr Val Val Thr Ala Lys His
20 25 30
Val Ser Gly Ser Asp Thr Met Ser Phe Gly Tyr Ala Lys Asn Thr Tyr
35 40 45
Thr Ala Val Gly Thr Asn Asn Asn Ser Gly Leu Asp Ile Lys Thr Arg
50 55 60
Arg Leu Ser Lys Leu Val Thr Glu Val Ala Pro Ala Glu Val Ser Asp
65 70 75 80
Ile Gly Ala Val Ser Gly Ala Tyr Gln Ala Gly Gly Arg Phe Thr Glu
85 90 95
Phe Tyr Arg Leu Gly Gly Gly Met Gln Tyr Val Lys Asp Lys Asn Gly
100 105 110
Asn Arg Thr Gln Val Tyr Thr Asn Gly Gly Phe Leu Val Gly Gly Thr
115 120 125
Val Ser Ala Leu Asn Ser Tyr Asn Asn Gly Gln Met Ile Thr Ala Gln
130 135 140
Thr Gly Asp Ile Phe Asn Pro Ala Asn Gly Pro Leu Ala Asn Tyr Leu
145 150 155 160
Asn Met Gly Asp Ser Gly Ser Pro Leu Phe Ala Tyr Asp Ser Leu Gln
165 170 175
Lys Lys Trp Val Leu Ile Gly Val Leu Ser Ser Gly Thr Asn Tyr Gly
180 185 190
Asn Asn Trp Val Val Thr Thr Gln Asp Phe Leu Gly Gln Gln Pro Gln
195 200 205
Asn Asp Phe Asp Lys Thr Ile Ala Tyr Thr Ser Gly Glu Gly Val Leu
210 215 220
Gln Trp Lys Tyr Asp Ala Ala Asn Gly Thr Gly Thr Leu Thr Gln Gly
225 230 235 240
Asn Thr Thr Trp Asp Met His Gly Lys Lys Gly Asn Asp Leu Asn Ala
245 250 255
Gly Lys Asn Leu Leu Phe Thr Gly Asn Asn Gly Glu Val Val Leu Gln
260 265 270
Asn Ser Val Asn Gln Gly Ala Gly Tyr Leu Gln Phe Ala Gly Asp Tyr
275 280 285
Arg Val Ser Ala Leu Asn Gly Gln Thr Trp Met Gly Gly Gly Ile Ile
290 295 300
Thr Asp Lys Gly Thr His Val Leu Trp Gln Val Asn Gly Val Ala Gly
305 310 315 320
Asp Asn Leu His Lys Thr Gly Glu Gly Thr Leu Thr Val Asn Gly Thr
325 330 335
Gly Val Asn Ala Gly Gly Leu Lys Val Gly Asp Gly Thr Val Ile Leu
340 345 350
Asn Gln Gln Ala Asp Ala Asp Gly Lys Val Gln Ala Phe Ser Ser Val
355 360 365
Gly Ile Ala Ser Gly Arg Pro Thr Val Val Leu Ser Asp Ser Gln Gln
370 375 380
Val Asn Pro Asp Asn Ile Ser Trp Gly Tyr Arg Gly Gly Arg Leu Glu
385 390 395 400
Leu Asn Gly Asn Asn Leu Thr Phe Thr Arg Leu Gln Ala Ala Asp Tyr
405 410 415
Gly Ala Ile Ile Thr Asn Asn Ser Glu Lys Lys Ser Thr Val Thr Leu
420 425 430
Asp Leu Gln Thr Leu Lys Ala Ser Asp Ile Asn Val Pro Val Asn Thr
435 440 445
Val Ser Ile Phe Gly Gly Arg Gly Ala Pro Gly Asp Leu Tyr Tyr Asp
450 455 460
Ser Ser Thr Lys Gln Tyr Phe Ile Leu Lys Ala Ser Ser Tyr Ser Pro
465 470 475 480
Phe Phe Ser Asp Leu Asn Asn Ser Ser Val Trp Gln Asn Val Gly Lys
485 490 495
Asp His Asn Lys Ala Ile Asp Thr Val Lys Gln Gln Lys Ile Glu Ala
500 505 510
Ser Ser Gln Pro Tyr Met Tyr His Gly Gln Leu Asn Gly Asn Met Asp
515 520 525
Val Asn Ile Pro Gln Leu Ser Gly Lys Asp Val Leu Ala Leu Asp Gly
530 535 540
Ser Val Asn Leu Pro Glu Gly Ser Ile Thr Lys Lys Ser Gly Thr Leu
545 550 555 560
Ile Phe Gln Gly His Pro Val Ile His Ala Gly Thr Thr Thr Ser Ser
565 570 575
Ser Gln Ser Asp Trp Glu Thr Arg Gln Phe Thr Leu Glu Lys Leu Lys
580 585 590
Leu Asp Ala Ala Thr Phe His Leu Ser Arg Asn Gly Lys Met Gln Gly
595 600 605
Asp Ile Asn Ala Thr Asn Gly Ser Thr Val Ile Leu Gly Ser Ser Arg
610 615 620
Val Phe Thr Asp Arg Ser Asp Gly Thr Gly Asn Ala Val Ser Ser Val
625 630 635 640
Glu Gly Ser Ala Thr Ala Thr Thr Val Gly Asp Gln Ser Asp Tyr Ser
645 650 655
Gly Asn Val Thr Leu Glu Asn Lys Ser Ser Leu Gln Ile Met Glu Arg
660 665 670
Phe Thr Gly Gly Ile Glu Ala Tyr Asp Ser Thr Val Ser Val Thr Ser
675 680 685
Gln Asn Ala Val Phe Asp Arg Val Gly Ser Phe Val Asn Ser Ser Leu
690 695 700
Thr Leu Gly Lys Gly Ala Lys Leu Thr Ala Gln Ser Gly Ile Phe Ser
705 710 715 720
Thr Gly Ala Val Asp Val Lys Glu Asn Ala Ser Leu Thr Leu Thr Gly
725 730 735
Met Pro Ser Ala Gln Lys Gln Gly Tyr Tyr Ser Pro Val Ile Ser Thr
740 745 750
Thr Glu Gly Ile Asn Leu Glu Asp Asn Ala Ser Phe Ser Val Lys Asn
755 760 765
Met Gly Tyr Leu Ser Ser Asp Ile His Ala Gly Thr Thr Ala Ala Thr
770 775 780
Ile Asn Leu Gly Asp Ser Asp Ala Asp Ala Gly Lys Thr Asp Ser Pro
785 790 795 800
Leu Phe Ser Ser Leu Met Lys Gly Tyr Asn Ala Val Leu Arg Gly Ser
805 810 815
Ile Thr Gly Ala Gln Ser Thr Val Asn Met Ile Asn Ala Leu Trp Tyr
820 825 830
Ser Asp Gly Lys Ser Glu Ala Gly Ala Leu Lys Ala Lys Gly Ser Arg
835 840 845
Ile Glu Leu Gly Asp Gly Lys His Phe Ala Thr Leu Gln Val Lys Glu
850 855 860
Leu Ser Ala Asp Asn Thr Thr Phe Leu Met His Thr Asn Asn Ser Arg
865 870 875 880
Ala Asp Gln Leu Asn Val Thr Asp Lys Leu Ser Gly Ser Asn Asn Ser
885 890 895
Val Leu Val Asp Phe Leu Asn Lys Pro Ala Ser Glu Met Ser Val Thr
900 905 910
Leu Ile Thr Ala Pro Lys Gly Ser Asp Glu Lys Thr Phe Thr Ala Gly
915 920 925
Thr Gln Gln Ile Gly Phe Ser Asn Val Thr Pro Val Ile Ser Thr Glu
930 935 940
Lys Thr Asp Asp Ala Thr Lys Trp Val Leu Thr Gly Tyr Gln Thr Thr
945 950 955 960
Ala Asp Ala Gly Ala Ser Lys Ala Ala Lys Asp Phe Met Ala Ser Gly
965 970 975
Tyr Lys Ser Phe Leu Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly
980 985 990
Asp Leu Arg Asp Thr Gln Gly Asp Ala Gly Val Trp Ala Arg Ile Met
995 1000 1005
Asn Gly Thr Gly Ser Ala Asp Gly Asp Tyr Ser Asp Asn Tyr Thr His
1010 1015 1020
Val Gln Ile Gly Val Asp Arg Lys His Glu Leu Asp Gly Val Asp Leu
1025 1030 1035 1040
Phe Thr Gly Ala Leu Leu Thr Tyr Thr Asp Ser Asn Ala Ser Ser His
1045 1050 1055
Ala Phe Ser Gly Lys Asn Lys Ser Val Gly Gly Gly Leu Tyr Ala Ser
1060 1065 1070
Ala Leu Phe Asn Ser Gly Ala Tyr Phe Asp Leu Ile Gly Lys Tyr Leu
1075 1080 1085
His His Asp Asn Gln His Thr Ala Asn Phe Ala Ser Leu Gly Thr Lys
1090 1095 1100
Asp Tyr Ser Ser His Ser Trp Tyr Ala Gly Ala Glu Val Gly Tyr Arg
1105 1110 1115 1120
Tyr His Leu Thr Lys Glu Ser Trp Val Glu Pro Gln Ile Glu Leu Val
1125 1130 1135
Tyr Gly Ser Val Ser Gly Lys Ala Phe Ser Trp Glu Asp Arg Gly Met
1140 1145 1150
Ala Leu Ser Met Lys Asp Lys Asp Tyr Asn Pro Leu Ile Gly Arg Thr
1155 1160 1165
Gly Val Asp Val Gly Arg Ala Phe Ser Gly Asp Asp Trp Lys Ile Thr
1170 1175 1180
Ala Arg Ala Gly Leu Gly Tyr Gln Phe Asp Leu Leu Ala Asn Gly Glu
1185 1190 1195 1200
Thr Val Leu Gln Asp Ala Ser Gly Glu Lys Arg Phe Glu Gly Glu Lys
1205 1210 1215
Asp Ser Arg Met Leu Met Thr Val Gly Met Asn Ala Glu Ile Lys Asp
1220 1225 1230
Asn Met Arg Leu Gly Leu Glu Leu Glu Lys Ser Ala Phe Gly Lys Tyr
1235 1240 1245
Asn Val Asp Asn Ala Ile Asn Ala Asn Phe Arg Tyr Val Phe
1250 1255 1260
<210> 114
<211> 887
<212> PRT
<213> 宋内志贺氏菌
<400> 114
Met Ser Glu Arg Phe Pro Asn Asp Val Asp Pro Ile Glu Thr Arg Asp
1 5 10 15
Trp Leu Gln Ala Ile Glu Ser Val Ile Arg Glu Glu Gly Val Glu Arg
20 25 30
Ala Gln Tyr Leu Ile Asp Gln Leu Leu Ala Glu Ala Arg Lys Gly Gly
35 40 45
Val Asn Val Ala Ala Gly Thr Gly Ile Ser Asn Tyr Ile Asn Thr Ile
50 55 60
Pro Val Glu Glu Gln Pro Glu Tyr Pro Gly Asn Leu Glu Leu Glu Arg
65 70 75 80
Arg Ile Arg Ser Ala Ile Arg Trp Asn Ala Ile Met Thr Val Leu Arg
85 90 95
Ala Ser Lys Lys Asp Leu Glu Leu Gly Gly His Met Ala Ser Phe Gln
100 105 110
Ser Ser Ala Thr Ile Tyr Asp Val Cys Phe Asn His Phe Phe Arg Ala
115 120 125
Arg Asn Glu Gln Asp Gly Gly Asp Leu Val Tyr Phe Gln Gly His Ile
130 135 140
Ser Pro Gly Val Tyr Ala Arg Ala Phe Leu Glu Gly Arg Leu Thr Gln
145 150 155 160
Glu Gln Leu Asp Asn Phe Arg Gln Glu Val His Gly Asn Gly Leu Ser
165 170 175
Ser Tyr Pro His Pro Lys Leu Met Pro Glu Phe Trp Gln Phe Pro Thr
180 185 190
Val Ser Met Gly Leu Gly Pro Ile Gly Ala Ile Tyr Gln Ala Lys Phe
195 200 205
Leu Lys Tyr Leu Glu His Arg Gly Leu Lys Asp Thr Ser Lys Gln Thr
210 215 220
Val Tyr Ala Phe Leu Gly Asp Gly Glu Met Asp Glu Pro Glu Ser Lys
225 230 235 240
Gly Ala Ile Thr Ile Ala Thr Arg Glu Lys Leu Asp Asn Leu Val Phe
245 250 255
Val Ile Asn Cys Asn Leu Gln Arg Leu Asp Gly Pro Val Thr Gly Asn
260 265 270
Gly Lys Ile Ile Asn Glu Leu Glu Gly Ile Phe Glu Gly Ala Gly Trp
275 280 285
Asn Val Ile Lys Val Met Trp Gly Ser Arg Trp Asp Glu Leu Leu Arg
290 295 300
Lys Asp Thr Ser Gly Lys Leu Ile Gln Leu Met Asn Glu Thr Val Asp
305 310 315 320
Gly Asp Tyr Gln Thr Phe Lys Ser Lys Asp Gly Ala Tyr Val Arg Glu
325 330 335
His Phe Phe Gly Lys Tyr Pro Glu Thr Ala Ala Leu Val Ala Asp Trp
340 345 350
Thr Asp Glu Gln Ile Trp Ala Leu Asn Arg Gly Gly His Asp Pro Lys
355 360 365
Lys Ile Tyr Ala Ala Phe Lys Lys Ala Gln Glu Thr Lys Gly Lys Ala
370 375 380
Thr Val Ile Leu Ala His Thr Ile Lys Gly Tyr Gly Met Gly Asp Ala
385 390 395 400
Ala Glu Gly Lys Asn Ile Ala His Gln Val Lys Lys Met Asn Met Asp
405 410 415
Gly Val Arg His Ile Arg Asp Arg Phe Asn Val Pro Val Ser Asp Ala
420 425 430
Asp Ile Glu Lys Leu Pro Tyr Ile Thr Phe Pro Glu Gly Ser Glu Glu
435 440 445
His Thr Tyr Leu His Ala Gln Arg Gln Lys Leu His Gly Tyr Leu Pro
450 455 460
Ser Arg Gln Pro Asn Phe Thr Glu Lys Leu Glu Leu Pro Ser Leu Gln
465 470 475 480
Asp Phe Gly Ala Leu Leu Glu Glu Gln Ser Lys Glu Ile Ser Thr Thr
485 490 495
Ile Ala Phe Val Arg Ala Leu Asn Val Met Leu Lys Asn Lys Ser Ile
500 505 510
Lys Asp Arg Leu Val Pro Ile Ile Ala Asp Glu Ala Arg Thr Phe Gly
515 520 525
Met Glu Gly Leu Phe Arg Gln Ile Gly Ile Tyr Ser Pro Asn Gly Gln
530 535 540
Gln Tyr Ile Pro Gln Asp Arg Glu Gln Val Ala Tyr Tyr Lys Glu Asp
545 550 555 560
Glu Lys Gly Gln Ile Leu Gln Glu Gly Ile Asn Glu Leu Gly Ala Gly
565 570 575
Cys Ser Trp Leu Ala Ala Ala Thr Ser Tyr Ser Thr Asn Asn Leu Pro
580 585 590
Met Ile Pro Phe Tyr Ile Tyr Tyr Ser Met Phe Gly Phe Gln Arg Ile
595 600 605
Gly Asp Leu Cys Trp Ala Ala Gly Asp Gln Gln Ala Arg Gly Phe Leu
610 615 620
Ile Gly Gly Thr Ser Gly Arg Thr Thr Leu Asn Gly Glu Gly Leu Gln
625 630 635 640
His Glu Asp Gly His Ser His Ile Gln Ser Leu Thr Ile Pro Asn Cys
645 650 655
Ile Ser Tyr Asp Pro Ala Tyr Ala Tyr Glu Val Ala Val Ile Met His
660 665 670
Asp Gly Leu Glu Arg Met Tyr Gly Glu Lys Gln Glu Asn Val Tyr Tyr
675 680 685
Tyr Ile Thr Thr Leu Asn Glu Asn Tyr His Met Pro Ala Met Pro Glu
690 695 700
Gly Ala Glu Glu Gly Ile Arg Lys Gly Ile Tyr Lys Leu Glu Thr Ile
705 710 715 720
Glu Gly Ser Lys Gly Lys Val Gln Leu Leu Gly Ser Gly Ser Ile Leu
725 730 735
Arg His Val Arg Glu Ala Ala Glu Ile Leu Ala Lys Asp Tyr Gly Val
740 745 750
Gly Ser Asp Val Tyr Ser Val Thr Ser Phe Thr Glu Leu Ala Arg Asp
755 760 765
Gly Gln Asp Cys Glu Arg Trp Asn Met Leu His Pro Leu Glu Thr Pro
770 775 780
Arg Val Pro Tyr Ile Ala Gln Val Met Asn Asp Ala Pro Ala Val Ala
785 790 795 800
Ser Thr Asp Tyr Met Lys Leu Phe Ala Glu Gln Val Arg Thr Tyr Val
805 810 815
Pro Ala Asp Asp Tyr Arg Val Leu Gly Thr Asp Gly Phe Gly Arg Ser
820 825 830
Asp Ser Arg Glu Asn Leu Arg His His Phe Glu Val Asp Ala Ser Tyr
835 840 845
Val Val Val Ala Ala Leu Gly Glu Leu Ala Lys Arg Gly Glu Ile Asp
850 855 860
Lys Lys Val Val Ala Asp Ala Ile Ala Lys Phe Asn Ile Asp Ala Asp
865 870 875 880
Lys Val Asn Pro Arg Leu Ala
885
<210> 115
<211> 729
<212> PRT
<213> 宋内志贺氏菌
<400> 115
Met Ala Arg Ser Lys Thr Ala Gln Pro Lys His Ser Leu Arg Lys Ile
1 5 10 15
Ala Val Val Val Ala Thr Ala Val Ser Gly Met Ser Val Tyr Ala Gln
20 25 30
Ala Ala Val Glu Pro Lys Glu Asp Thr Ile Thr Val Thr Ala Ala Pro
35 40 45
Ala Pro Gln Glu Ser Ala Trp Gly Pro Ala Ala Thr Ile Ala Ala Lys
50 55 60
His Ser Ala Thr Ala Thr Lys Thr Asp Thr Pro Ile Glu Lys Thr Pro
65 70 75 80
Gln Ser Ile Ser Val Val Thr Asn Glu Glu Met Gln Met Arg Gln Phe
85 90 95
Gln Ser Val Lys Glu Ala Leu Gly Tyr Thr Pro Gly Val Thr Val Ser
100 105 110
Ser Arg Gly Ala Ser Asn Thr Tyr Asp Phe Val Ile Ile Arg Gly Phe
115 120 125
Ser Ser Val Gly Leu Asn Gln Asn Asn Tyr Leu Asp Gly Leu Lys Leu
130 135 140
Gln Gly Asn Phe Tyr Asn Asp Ala Val Ile Asp Pro Tyr Met Leu Glu
145 150 155 160
Arg Val Glu Leu Met Arg Gly Pro Thr Ser Val Leu Tyr Gly Lys Ser
165 170 175
Asn Pro Gly Gly Ile Ile Ser Met Val Ser Lys Arg Pro Thr Thr Glu
180 185 190
Pro Leu Lys Glu Ile Gln Phe Lys Met Gly Thr Asp Asn Leu Phe Gln
195 200 205
Thr Gly Phe Asp Phe Ser Asp Ala Leu Asp Asp Asn Gly Glu Phe Ser
210 215 220
Tyr Arg Leu Thr Gly Leu Ala Arg Ser Thr Asn Glu Gln Gln Lys Asn
225 230 235 240
Ser Glu Ser Gln Arg Tyr Thr Ile Ala Pro Ser Phe Ser Trp Arg Pro
245 250 255
Asp Asp Lys Thr Asn Phe Thr Phe Leu Ser Tyr Phe Gln Asn Glu Pro
260 265 270
Glu Thr Gly Tyr Tyr Gly Trp Leu Pro Lys Glu Gly Thr Val Glu Pro
275 280 285
Leu Pro Asn Gly Lys Arg Leu Pro Thr Asp Phe Asn Glu Gly Ala Ser
290 295 300
Asn Asn Thr Tyr Ser Arg Asn Gln Lys Met Val Gly Tyr Ser Phe Glu
305 310 315 320
His Gly Phe Asn Asp Thr Phe Thr Val Arg Gln Asn Leu Arg Phe Ser
325 330 335
Glu Met Lys Thr Ser Gln Lys Ser Val Tyr Gly Thr Gly Ile Ala Gly
340 345 350
Asp Gly His Thr Leu Asn Arg Gly Thr Val Val Asp Asn Glu Arg Leu
355 360 365
Gln Asn Phe Ser Val Asp Thr Gln Leu Glu Ser Lys Phe Ala Thr Gly
370 375 380
Asp Val Glu His Thr Leu Leu Thr Gly Val Asp Phe Met Arg Met Arg
385 390 395 400
Asn Asp Ile Asn Ala Ser Phe Gly Ser Ala Pro Ser Ile Asp Leu Tyr
405 410 415
Asn Lys Tyr His Pro Glu Tyr Phe Ala Phe Gly Ser Ala Glu Pro Tyr
420 425 430
Gln Met Asn Glu Ser Lys Gln Thr Gly Ile Tyr Val Gln Asp Gln Ala
435 440 445
Glu Trp Asn Lys Trp Val Phe Thr Leu Gly Gly Arg Tyr Asp Trp Ser
450 455 460
Lys Gln Ala Thr Thr Val Arg Glu Asn Ser Tyr Thr Pro Thr Glu Gly
465 470 475 480
Tyr Ile Glu Arg Asn Asp His Gln Phe Thr Trp Arg Gly Gly Val Asn
485 490 495
Tyr Leu Phe Asp Asn Gly Ile Ser Pro Tyr Phe Ser Tyr Ser Gln Ser
500 505 510
Phe Glu Pro Ser Ala Phe Asp Leu Trp Ser Asn Pro Arg Ile Ser Tyr
515 520 525
Lys Pro Ser Lys Gly Glu Gln Tyr Glu Ala Gly Val Lys Tyr Val Pro
530 535 540
Asn Asp Met Pro Val Val Val Thr Gly Ala Val Tyr Gln Leu Thr Lys
545 550 555 560
Thr Asn Asn Leu Thr Ala Asp Pro Thr Asn Pro Leu Ala Gln Val Pro
565 570 575
Ala Gly Glu Ile Arg Ala Arg Gly Val Glu Leu Glu Ala Lys Ala Ala
580 585 590
Leu Asn Ala Asn Ile Asn Leu Thr Ala Ser Tyr Thr Tyr Thr Asp Ala
595 600 605
Glu Tyr Thr Lys Asp Thr Asn Leu Lys Gly Lys Thr Pro Glu Gln Val
610 615 620
Pro Glu His Met Ala Ser Leu Trp Gly Asp Tyr Thr Phe Asn Glu Gly
625 630 635 640
Pro Leu Ser Gly Leu Thr Leu Gly Thr Gly Gly Arg Phe Ile Gly Ser
645 650 655
Ser Tyr Gly Asp Pro Ala Asn Thr Phe Lys Val Gly Ser Thr Ala Val
660 665 670
Met Asp Ala Val Val Lys Tyr Asp Leu Ala Arg Phe Gly Met Ala Gly
675 680 685
Ser Ser Leu Ala Val Asn Val Asn Asn Leu Leu Asp Arg Glu Tyr Val
690 695 700
Ala Ser Cys Phe Gln Thr Tyr Gly Cys Phe Trp Gly Ala Glu Arg Gln
705 710 715 720
Val Val Ala Thr Ala Thr Phe Arg Phe
725
<210> 116
<211> 746
<212> PRT
<213> 宋内志贺氏菌
<400> 116
Met Asn Lys Lys Ile His Ser Leu Ala Leu Leu Val Asn Leu Gly Ile
1 5 10 15
Tyr Gly Val Ala Gln Ala Gln Glu Pro Thr Asp Thr Pro Val Ser His
20 25 30
Asp Asp Thr Ile Val Val Thr Ala Ala Glu Gln Asn Leu Gln Ala Pro
35 40 45
Gly Val Ser Thr Ile Thr Ala Asp Glu Ile Arg Lys Asn Pro Val Ala
50 55 60
Arg Asp Val Ser Glu Ile Ile Arg Thr Met Pro Gly Val Asn Leu Thr
65 70 75 80
Gly Asn Ser Thr Ser Gly Gln Arg Gly Asn Asn Arg Gln Ile Asp Ile
85 90 95
Arg Gly Met Gly Pro Glu Asn Thr Leu Ile Leu Ile Asp Gly Lys Pro
100 105 110
Val Ser Ser Arg Asn Ser Val Arg Gln Gly Trp Arg Gly Glu Arg Asp
115 120 125
Thr Arg Gly Asp Thr Ser Trp Val Pro Pro Glu Met Ile Glu Arg Ile
130 135 140
Glu Val Leu Arg Gly Pro Ala Ala Ala Arg Tyr Gly Asn Gly Ala Ala
145 150 155 160
Gly Gly Val Val Asn Ile Ile Thr Lys Lys Gly Ser Gly Glu Trp His
165 170 175
Gly Ser Trp Asp Ala Tyr Phe Asn Ala Pro Glu His Lys Glu Glu Gly
180 185 190
Ala Thr Lys Arg Thr Asn Phe Ser Leu Thr Gly Pro Leu Gly Asp Glu
195 200 205
Phe Ser Phe Arg Leu Tyr Gly Asn Leu Asp Lys Thr Gln Ala Asp Ala
210 215 220
Trp Asp Ile Asn Gln Gly His Gln Ser Ala Arg Ala Gly Thr Tyr Ala
225 230 235 240
Thr Thr Leu Pro Ala Gly Arg Glu Gly Val Ile Asn Lys Asp Ile Asn
245 250 255
Gly Val Val Arg Trp Asp Phe Ala Pro Leu Gln Ser Leu Glu Leu Glu
260 265 270
Ala Gly Tyr Ser Arg Gln Gly Asn Leu Tyr Ala Gly Asp Thr Gln Asn
275 280 285
Thr Asn Ser Asp Ser Tyr Thr Arg Ser Lys Tyr Gly Asp Glu Thr Asn
290 295 300
Arg Leu Tyr Arg Gln Asn Tyr Ser Leu Thr Trp Asn Gly Gly Trp Asp
305 310 315 320
Asn Gly Val Thr Thr Ser Asn Trp Val Gln Tyr Glu His Thr Arg Asn
325 330 335
Ser Arg Ile Pro Glu Gly Leu Ala Gly Gly Thr Glu Gly Lys Phe Asn
340 345 350
Glu Lys Ala Thr Gln Asp Phe Val Asp Ile Asp Leu Asp Asp Val Met
355 360 365
Leu His Ser Glu Val Asn Leu Pro Ile Asp Phe Leu Val Asn Gln Thr
370 375 380
Leu Thr Leu Gly Thr Glu Trp Asn Gln Gln Arg Met Lys Asp Leu Ser
385 390 395 400
Ser Asn Thr Gln Ala Leu Thr Gly Ala Asn Thr Gly Gly Ala Ile Asp
405 410 415
Gly Val Ser Ala Thr Asp Arg Ser Pro Tyr Ser Lys Ala Glu Ile Phe
420 425 430
Ser Leu Phe Ala Glu Asn Asn Met Glu Leu Thr Asp Ser Thr Ile Val
435 440 445
Thr Pro Gly Leu Arg Phe Asp His His Ser Ile Val Gly Asn Asn Trp
450 455 460
Ser Pro Ala Leu Asn Ile Ser Gln Gly Leu Gly Asp Asp Phe Thr Leu
465 470 475 480
Lys Met Gly Ile Ala Arg Ala Tyr Lys Ala Pro Ser Leu Tyr Gln Thr
485 490 495
Asn Pro Asn Tyr Ile Leu Tyr Ser Lys Gly Gln Gly Cys Tyr Ala Ser
500 505 510
Ala Gly Gly Cys Tyr Gln Gln Gly Asn Asp Asp Leu Lys Ala Glu Thr
515 520 525
Ser Ile Asn Lys Glu Ile Gly Leu Glu Phe Lys Arg Asp Gly Trp Leu
530 535 540
Ala Gly Val Thr Trp Phe Arg Asn Asp Tyr Arg Asn Lys Ile Glu Ala
545 550 555 560
Gly Tyr Val Ala Val Gly Gln Asn Ala Val Gly Thr Asp Leu Tyr Gln
565 570 575
Trp Asp Asn Val Pro Lys Ala Val Val Glu Gly Leu Glu Gly Ser Leu
580 585 590
Asn Val Pro Val Ser Glu Met Val Met Trp Thr Asn Asn Ile Thr Tyr
595 600 605
Met Leu Lys Ser Glu Asn Lys Thr Thr Gly Asp Arg Leu Ser Ile Ile
610 615 620
Pro Glu Tyr Thr Leu Asn Ser Thr Leu Ser Trp Gln Ala Arg Glu Asp
625 630 635 640
Leu Ser Met Gln Thr Thr Phe Thr Trp Tyr Gly Lys Gln Gln Pro Lys
645 650 655
Lys Tyr Asn Tyr Lys Gly Gln Pro Ala Val Gly Pro Glu Thr Lys Glu
660 665 670
Ile Ser Pro Tyr Ser Ile Val Gly Leu Ser Ala Thr Trp Asp Val Thr
675 680 685
Lys Asn Val Ser Leu Thr Gly Gly Val Asp Asn Leu Phe Asp Lys Arg
690 695 700
Leu Trp Arg Ala Gly Asn Ala Gln Thr Thr Gly Asp Leu Ala Gly Ala
705 710 715 720
Asn Tyr Ile Ala Gly Ala Gly Ala Tyr Thr Tyr Asn Glu Pro Gly Arg
725 730 735
Thr Trp Tyr Met Ser Val Asn Thr His Phe
740 745
<210> 117
<211> 682
<212> PRT
<213> 宋内志贺氏菌
<400> 117
Met Asn Met Phe Phe Arg Leu Thr Ala Leu Ala Gly Leu Leu Ala Ile
1 5 10 15
Ala Gly Gln Thr Phe Ala Val Glu Asp Ile Thr Arg Ala Asp Gln Ile
20 25 30
Pro Val Leu Lys Glu Glu Thr Gln His Ala Thr Val Ser Glu Arg Val
35 40 45
Thr Ser Arg Phe Thr Arg Ser His Tyr Arg Gln Phe Asp Leu Asp Gln
50 55 60
Ala Phe Ser Ala Lys Ile Phe Asp Arg Tyr Leu Asn Leu Leu Asp Tyr
65 70 75 80
Ser His Asn Val Leu Leu Ala Ser Asp Val Glu Gln Phe Ala Lys Lys
85 90 95
Lys Thr Glu Leu Gly Asp Glu Leu Arg Ser Gly Lys Leu Asp Val Phe
100 105 110
Tyr Asp Leu Tyr Asn Leu Ala Gln Lys Arg Arg Phe Glu Arg Tyr Gln
115 120 125
Tyr Ala Leu Ser Val Leu Glu Lys Pro Met Asp Phe Thr Gly Asn Asp
130 135 140
Thr Tyr Asn Leu Asp Arg Ser Lys Ala Pro Trp Pro Lys Asn Glu Ala
145 150 155 160
Glu Leu Asn Ala Leu Trp Asp Ser Lys Val Lys Phe Asp Glu Leu Ser
165 170 175
Leu Lys Leu Ala Gly Lys Thr Asp Lys Glu Ile Arg Glu Thr Leu Thr
180 185 190
Arg Arg Tyr Lys Phe Ala Ile Arg Arg Leu Ala Gln Thr Asn Ser Glu
195 200 205
Asp Val Phe Ser Leu Ala Met Thr Ala Phe Ala Arg Glu Ile Asp Pro
210 215 220
His Thr Asn Tyr Leu Ser Pro Arg Asn Thr Glu Gln Phe Asn Thr Glu
225 230 235 240
Met Ser Leu Ser Leu Glu Gly Ile Gly Ala Val Leu Gln Met Asp Asp
245 250 255
Asp Tyr Thr Val Ile Asn Ser Met Val Ala Gly Gly Pro Ala Ala Lys
260 265 270
Ser Lys Ala Ile Ser Val Gly Asp Lys Ile Val Gly Val Gly Gln Thr
275 280 285
Gly Lys Pro Met Val Asp Val Ile Gly Trp Arg Leu Asp Asp Val Val
290 295 300
Ala Leu Ile Lys Gly Pro Lys Gly Ser Lys Val Arg Leu Glu Ile Leu
305 310 315 320
Pro Ala Gly Lys Gly Thr Lys Thr Arg Thr Val Thr Leu Thr Arg Glu
325 330 335
Arg Ile Arg Leu Glu Asp Arg Ala Val Lys Met Ser Val Lys Thr Val
340 345 350
Gly Lys Glu Lys Val Gly Val Leu Asp Ile Pro Gly Phe Tyr Val Gly
355 360 365
Leu Thr Asp Asp Val Lys Val Gln Leu Gln Lys Leu Glu Lys Gln Asn
370 375 380
Val Ser Ser Val Ile Ile Asp Leu Arg Ser Asn Gly Gly Gly Ala Leu
385 390 395 400
Thr Glu Ala Val Ser Leu Ser Gly Leu Phe Ile Pro Ala Gly Pro Ile
405 410 415
Val Gln Val Arg Asp Asn Asn Gly Lys Val Arg Glu Asp Ser Asp Thr
420 425 430
Asp Gly Gln Val Phe Tyr Lys Gly Pro Leu Val Val Leu Val Asp Arg
435 440 445
Phe Ser Ala Ser Ala Ser Glu Ile Phe Ala Ala Ala Met Gln Asp Tyr
450 455 460
Gly Arg Ala Leu Val Val Gly Glu Pro Thr Phe Gly Lys Gly Thr Val
465 470 475 480
Gln Gln Tyr Arg Ser Leu Asn Arg Ile Tyr Asp Gln Met Leu Arg Pro
485 490 495
Glu Trp Pro Ala Leu Gly Ser Val Gln Tyr Thr Ile Gln Lys Phe Tyr
500 505 510
Arg Val Asn Gly Gly Ser Thr Gln Arg Lys Gly Val Thr Pro Asp Ile
515 520 525
Ile Met Pro Thr Gly Asn Glu Glu Thr Glu Thr Gly Glu Lys Phe Glu
530 535 540
Asp Asn Ala Leu Pro Trp Asp Ser Ile Asp Ala Ala Thr Tyr Val Lys
545 550 555 560
Ser Gly Asp Leu Thr Ala Phe Gly Pro Glu Leu Leu Lys Glu His Asn
565 570 575
Ala Arg Ile Ala Lys Asp Pro Glu Phe Gln Asn Ile Met Lys Asp Ile
580 585 590
Ala Arg Phe Asn Ala Met Arg Asp Lys Arg Asn Ile Val Ser Leu Asn
595 600 605
Tyr Ala Val Arg Glu Lys Glu Asn Asn Glu Asp Asp Ala Thr Arg Leu
610 615 620
Ala Arg Leu Asn Glu Arg Phe Lys Arg Glu Gly Lys Pro Glu Leu Lys
625 630 635 640
Lys Leu Asp Asp Leu Pro Lys Asp Tyr Gln Glu Pro Asp Pro Tyr Leu
645 650 655
Asp Glu Thr Val Asn Ile Ala Leu Asp Leu Ala Lys Leu Glu Lys Ala
660 665 670
Arg Pro Ala Glu Gln Pro Ala Pro Val Lys
675 680
<210> 118
<211> 212
<212> PRT
<213> 宋内志贺氏菌
<400> 118
Met Lys Lys Leu Thr Val Ala Ala Leu Ala Val Thr Thr Leu Leu Ser
1 5 10 15
Gly Ser Ala Phe Ala His Glu Ala Gly Glu Phe Phe Met Arg Ala Gly
20 25 30
Ser Ala Thr Val Arg Pro Thr Glu Gly Ala Gly Gly Thr Leu Gly Ser
35 40 45
Leu Gly Gly Phe Ser Val Thr Asn Asn Thr Gln Leu Gly Leu Thr Phe
50 55 60
Thr Tyr Met Ala Thr Asp Asn Ile Gly Val Glu Leu Leu Ala Ala Thr
65 70 75 80
Pro Phe Arg His Lys Ile Gly Thr Arg Ala Thr Gly Asp Ile Ala Thr
85 90 95
Val His His Leu Pro Pro Thr Leu Met Ala Gln Trp Tyr Phe Gly Asp
100 105 110
Ala Gly Ser Lys Phe Arg Pro Tyr Val Gly Ala Gly Ile Asn Tyr Thr
115 120 125
Thr Phe Phe Asp Asn Gly Phe Asn Asp His Gly Lys Glu Ala Gly Leu
130 135 140
Ser Asp Leu Ser Leu Lys Asp Tyr Trp Gly Ala Ala Gly Gln Val Gly
145 150 155 160
Val Asp Tyr Leu Ile Asn Arg Asp Trp Leu Val Asn Met Ser Val Trp
165 170 175
Tyr Met Asp Ile Asp Thr Thr Ala Lys Tyr Lys Ser Gly Val Thr Thr
180 185 190
Val Lys Asp Ser Val Arg Leu Asp Pro Trp Val Phe Met Phe Ser Ala
195 200 205
Gly Tyr Arg Phe
210
<210> 119
<211> 315
<212> PRT
<213> 宋内志贺氏菌
<400> 119
Met Pro Asp Met Lys Leu Phe Ala Gly Asn Ala Thr Pro Glu Leu Ala
1 5 10 15
Gln Arg Ile Ala Asn Arg Leu Tyr Thr Ser Leu Gly Asp Ala Ala Val
20 25 30
Gly Arg Phe Ser Asp Gly Glu Val Ser Val Gln Ile Asn Glu Asn Val
35 40 45
Arg Gly Gly Asp Ile Phe Ile Ile Gln Ser Thr Cys Ala Pro Thr Asn
50 55 60
Asp Asn Leu Met Glu Leu Val Val Met Val Asp Ala Leu Arg Arg Ala
65 70 75 80
Ser Ala Gly Arg Ile Thr Ala Val Ile Pro Tyr Phe Gly Tyr Ala Arg
85 90 95
Gln Asp Arg Arg Val Arg Ser Ala Arg Val Pro Ile Thr Ala Lys Val
100 105 110
Val Ala Asp Phe Leu Ser Ser Val Gly Val Asp Arg Val Leu Thr Val
115 120 125
Asp Leu His Ala Glu Gln Ile Gln Gly Phe Phe Asp Val Pro Val Asp
130 135 140
Asn Val Phe Gly Ser Pro Ile Leu Leu Glu Asp Met Leu Gln Leu Asn
145 150 155 160
Leu Asp Asn Pro Ile Val Val Ser Pro Asp Ile Gly Gly Val Val Arg
165 170 175
Ala Arg Ala Ile Ala Lys Leu Leu Asn Asp Thr Asp Met Ala Ile Ile
180 185 190
Asp Lys Arg Arg Pro Arg Ala Asn Val Ser Gln Val Met His Ile Ile
195 200 205
Gly Asp Val Ala Gly Arg Asp Cys Val Leu Val Asp Asp Met Ile Asp
210 215 220
Thr Gly Gly Thr Leu Cys Lys Ala Ala Glu Ala Leu Lys Glu Arg Gly
225 230 235 240
Ala Lys Arg Val Phe Ala Tyr Ala Thr His Pro Ile Phe Ser Gly Asn
245 250 255
Ala Ala Asn Asn Leu Arg Asn Ser Val Ile Asp Glu Val Val Val Cys
260 265 270
Asp Thr Ile Pro Leu Ser Asp Glu Ile Lys Ser Leu Pro Asn Val Arg
275 280 285
Thr Leu Thr Leu Ser Gly Met Leu Ala Glu Ala Ile Arg Arg Ile Ser
290 295 300
Asn Glu Glu Ser Ile Ser Ala Met Phe Glu His
305 310 315
<210> 120
<211> 663
<212> PRT
<213> 宋内志贺氏菌
<400> 120
Met Phe Arg Leu Asn Pro Phe Val Arg Val Gly Leu Cys Leu Ser Ala
1 5 10 15
Ile Ser Cys Val Trp Pro Val Leu Ala Val Asp Asp Asp Gly Glu Thr
20 25 30
Met Val Val Thr Ala Ser Ser Val Glu Gln Asn Leu Lys Asp Ala Pro
35 40 45
Ala Ser Ile Ser Val Ile Thr Gln Glu Asp Leu Gln Arg Lys Pro Val
50 55 60
Gln Asn Leu Lys Asp Val Leu Lys Glu Val Pro Gly Val Gln Leu Thr
65 70 75 80
Asn Glu Gly Asp Asn Arg Lys Gly Val Ser Ile Arg Gly Leu Asp Ser
85 90 95
Ser Tyr Thr Leu Ile Leu Val Asp Gly Lys Arg Val Asn Ser Arg Asn
100 105 110
Ala Val Phe Arg His Asn Asp Phe Asp Leu Asn Trp Ile Pro Val Asp
115 120 125
Ser Ile Glu Arg Ile Glu Val Val Arg Gly Pro Met Ser Ser Leu Tyr
130 135 140
Gly Ser Asp Ala Leu Gly Gly Val Val Asn Ile Ile Thr Lys Lys Ile
145 150 155 160
Gly Gln Lys Trp Ser Gly Thr Val Thr Val Asp Thr Thr Ile Gln Glu
165 170 175
His Arg Asp Arg Gly Asp Thr Tyr Asn Gly Gln Phe Phe Thr Ser Gly
180 185 190
Pro Leu Ile Asp Gly Val Leu Gly Met Lys Ala Tyr Gly Ser Leu Ala
195 200 205
Lys Arg Glu Lys Asp Asp Pro Gln Asn Ser Thr Thr Thr Asp Thr Gly
210 215 220
Glu Thr Pro Arg Ile Glu Gly Phe Ser Ser Arg Asp Gly Asn Val Glu
225 230 235 240
Phe Ala Trp Thr Pro Asn Gln Asn His Asp Phe Thr Ala Gly Tyr Gly
245 250 255
Phe Asp Arg Gln Asp Arg Asp Ser Asp Ser Leu Asp Lys Asn Arg Leu
260 265 270
Glu Arg Gln Asn Tyr Ser Val Ser His Asn Gly Arg Trp Asp Tyr Gly
275 280 285
Thr Ser Glu Leu Lys Tyr Tyr Gly Glu Lys Val Glu Asn Lys Asn Pro
290 295 300
Gly Asn Ser Ser Pro Ile Thr Ser Glu Ser Asn Thr Val Asp Gly Lys
305 310 315 320
Tyr Thr Leu Pro Leu Thr Ala Ile Asn Gln Phe Leu Thr Val Gly Gly
325 330 335
Glu Trp Arg His Asp Lys Leu Ser Asp Ala Val Asn Leu Thr Gly Gly
340 345 350
Thr Ser Ser Lys Thr Ser Ala Ser Gln Tyr Ala Leu Phe Val Glu Asp
355 360 365
Glu Trp Arg Ile Phe Glu Pro Leu Ala Leu Thr Thr Gly Val Arg Met
370 375 380
Asp Asp His Glu Thr Tyr Gly Glu His Trp Ser Pro Arg Ala Tyr Leu
385 390 395 400
Val Tyr Asn Ala Thr Asp Thr Val Thr Val Lys Gly Gly Trp Ala Thr
405 410 415
Ala Phe Lys Ala Pro Ser Leu Leu Gln Leu Ser Pro Asp Trp Thr Ser
420 425 430
Asn Ser Cys Arg Gly Ala Cys Lys Ile Val Gly Ser Pro Asp Leu Lys
435 440 445
Pro Glu Thr Ser Glu Ser Trp Glu Leu Gly Leu Tyr Tyr Met Gly Glu
450 455 460
Glu Gly Trp Leu Glu Gly Val Glu Ser Ser Val Thr Val Phe Arg Asn
465 470 475 480
Asp Val Lys Asp Arg Ile Ser Ile Ser Arg Thr Ser Asp Val Asn Ala
485 490 495
Ala Pro Gly Tyr Gln Asn Phe Val Gly Phe Glu Thr Gly Ala Asn Gly
500 505 510
Arg Arg Ile Pro Val Phe Ser Tyr Tyr Asn Val Asn Lys Ala Arg Ile
515 520 525
Gln Gly Val Glu Thr Glu Leu Lys Ile Pro Phe Asn Asp Glu Trp Lys
530 535 540
Leu Ser Ile Asn Tyr Thr Tyr Asn Asp Gly Arg Asp Val Ser Asn Gly
545 550 555 560
Glu Asn Lys Pro Leu Ser Asp Leu Pro Phe His Thr Ala Asn Gly Thr
565 570 575
Leu Asp Trp Lys Pro Leu Ala Leu Glu Asp Trp Ser Phe Tyr Val Ser
580 585 590
Gly His Tyr Thr Gly Gln Lys Arg Ala Asp Ser Ala Thr Ala Lys Thr
595 600 605
Pro Gly Gly Tyr Thr Ile Trp Asn Thr Gly Ala Ala Trp Gln Val Thr
610 615 620
Lys Asp Val Lys Leu Arg Ala Gly Val Leu Asn Leu Gly Asp Lys Asp
625 630 635 640
Leu Ser Arg Asp Asp Tyr Ser Tyr Asn Glu Asp Gly Arg Arg Tyr Phe
645 650 655
Met Ala Val Asp Tyr Arg Phe
660
<210> 121
<211> 358
<212> PRT
<213> 宋内志贺氏菌
<400> 121
Met Lys Leu Lys Leu Lys Asn Leu Ser Met Ala Ile Met Met Ser Thr
1 5 10 15
Ile Val Met Gly Ser Ser Ala Met Ala Ala Asp Ser Asn Glu Lys Ile
20 25 30
Val Ile Ala His Arg Gly Ala Ser Gly Tyr Leu Pro Glu His Thr Leu
35 40 45
Pro Ala Lys Ala Met Ala Tyr Ala Gln Gly Ala Asp Tyr Leu Glu Gln
50 55 60
Asp Leu Val Met Thr Lys Asp Asp His Leu Val Val Leu His Asp His
65 70 75 80
Tyr Leu Asp Arg Val Thr Asp Val Ala Asp Arg Phe Pro Asp Arg Ala
85 90 95
Arg Lys Asp Gly Arg Tyr Tyr Ala Ile Asp Phe Thr Leu Asp Glu Ile
100 105 110
Lys Ser Leu Lys Phe Thr Glu Gly Phe Asp Ile Glu Asn Gly Lys Lys
115 120 125
Val Gln Thr Tyr Pro Gly Arg Phe Pro Met Gly Lys Ser Asp Phe Arg
130 135 140
Gly His Thr Phe Glu Glu Glu Ile Glu Phe Val Gln Gly Leu Asn His
145 150 155 160
Ser Thr Gly Lys Asn Ile Gly Ile Tyr Pro Glu Ile Lys Ala Pro Trp
165 170 175
Phe His His Gln Glu Gly Lys Asp Ile Ala Ala Lys Thr Leu Glu Val
180 185 190
Leu Lys Lys Tyr Gly Tyr Thr Gly Lys Asp Asp Lys Val Tyr Leu Gln
195 200 205
Cys Phe Asp Ala Asp Glu Leu Lys Arg Ile Lys Asn Glu Leu Glu Pro
210 215 220
Lys Met Gly Met Asp Leu Asn Leu Val Gln Leu Ile Ala Tyr Thr Asp
225 230 235 240
Trp Asn Glu Thr Gln Gln Lys Gln Pro Asp Gly Ser Trp Val Asn Tyr
245 250 255
Ser Tyr Asp Trp Met Phe Lys Pro Gly Ala Met Lys Gln Val Ala Glu
260 265 270
Tyr Ala Asp Gly Ile Gly Pro Asp Tyr His Met Leu Ile Glu Glu Thr
275 280 285
Ser Gln Pro Gly Asn Ile Lys Leu Thr Gly Met Val Gln Asp Ala Gln
290 295 300
Gln Asn Lys Leu Val Val His Pro Tyr Thr Val Arg Ser Asp Lys Leu
305 310 315 320
Pro Glu Tyr Thr Thr Asp Val Asn Gln Leu Tyr Asp Ala Leu Tyr Asn
325 330 335
Lys Ala Gly Val Asn Gly Leu Phe Thr Asp Phe Pro Asp Lys Ala Val
340 345 350
Lys Phe Leu Asn Lys Glu
355
<210> 122
<211> 316
<212> PRT
<213> 宋内志贺氏菌
<400> 122
Met Asn Glu Leu Asp Gly Ile Lys Gln Phe Thr Thr Val Val Ala Asp
1 5 10 15
Ser Gly Asp Ile Glu Ser Ile Arg His Tyr His Pro Gln Asp Ala Thr
20 25 30
Thr Asn Pro Ser Leu Leu Leu Lys Ala Ala Gly Leu Ser Gln Tyr Glu
35 40 45
His Leu Ile Asp Asp Ala Ile Ala Trp Gly Lys Lys Asn Gly Lys Thr
50 55 60
Gln Glu Gln Gln Val Val Ala Ala Cys Asp Lys Leu Ala Val Asn Phe
65 70 75 80
Gly Ala Glu Ile Leu Lys Ile Val Pro Gly Arg Val Ser Thr Glu Val
85 90 95
Asp Ala Arg Leu Ser Phe Asp Lys Glu Lys Ser Ile Glu Lys Ala Arg
100 105 110
His Leu Val Asp Leu Tyr Gln Gln Gln Gly Val Glu Lys Ser Arg Ile
115 120 125
Leu Ile Lys Leu Ala Ser Thr Trp Glu Gly Ile Arg Ala Ala Glu Glu
130 135 140
Leu Glu Lys Glu Gly Ile Asn Cys Asn Leu Thr Leu Leu Phe Ser Phe
145 150 155 160
Ala Gln Ala Arg Ala Cys Ala Glu Ala Gly Val Phe Leu Ile Ser Pro
165 170 175
Phe Val Gly Arg Ile Tyr Asp Trp Tyr Gln Ala Arg Lys Pro Met Asp
180 185 190
Pro Tyr Val Val Glu Glu Asp Pro Gly Val Lys Ser Val Arg Asn Ile
195 200 205
Tyr Asp Tyr Tyr Lys Gln His His Tyr Glu Thr Ile Val Met Gly Ala
210 215 220
Ser Phe Arg Arg Thr Glu Gln Ile Leu Ala Leu Thr Gly Cys Asp Arg
225 230 235 240
Leu Thr Ile Ala Pro Asn Leu Leu Lys Glu Leu Gln Glu Lys Val Ser
245 250 255
Pro Val Val Arg Lys Leu Ile Pro Pro Ser Gln Thr Phe Pro Arg Pro
260 265 270
Ala Pro Met Ser Glu Ala Glu Phe Arg Trp Glu His Asn Gln Tyr Ala
275 280 285
Met Ala Val Glu Lys Leu Ser Glu Gly Ile Arg Leu Phe Ala Val Asp
290 295 300
Gln Arg Lys Leu Glu Asp Leu Leu Ala Ala Lys Leu
305 310 315
<210> 123
<211> 455
<212> PRT
<213> 宋内志贺氏菌
<400> 123
Met Lys Lys Gln Pro Gln Leu Leu Ser Ala Leu Ala Leu Ser Val Gly
1 5 10 15
Leu Thr Leu Ser Ala Ser Phe Gln Ala Val Ala Ser Ile Pro Gly Gln
20 25 30
Val Ala Asp Gln Ala Pro Leu Pro Ser Leu Ala Pro Met Leu Glu Lys
35 40 45
Val Leu Pro Ala Val Val Ser Val Arg Val Glu Gly Thr Ala Ser Gln
50 55 60
Gly Gln Lys Ile Pro Glu Glu Phe Lys Lys Phe Phe Gly Asp Asp Leu
65 70 75 80
Pro Glu Gln Pro Ala Gln Pro Phe Glu Gly Leu Gly Ser Gly Val Ile
85 90 95
Ile Asn Ala Ser Lys Gly Tyr Val Leu Thr Asn Asn His Val Ile Asn
100 105 110
Gln Ala Gln Lys Ile Ser Ile Gln Leu Asn Asp Gly Arg Glu Phe Asp
115 120 125
Ala Lys Leu Ile Gly Ser Asp Asp Gln Ser Asp Ile Ala Leu Leu Gln
130 135 140
Ile Gln Asn Pro Ser Lys Leu Thr Gln Ile Ala Ile Ala Asp Ser Asp
145 150 155 160
Lys Leu Arg Val Gly Asp Phe Ala Val Ala Val Gly Asn Pro Phe Gly
165 170 175
Leu Gly Gln Thr Ala Thr Ser Gly Ile Val Ser Ala Leu Gly Arg Ser
180 185 190
Gly Leu Asn Leu Glu Gly Leu Glu Asn Phe Ile Gln Thr Asp Ala Ser
195 200 205
Ile Asn Arg Gly Asn Ser Gly Gly Ala Leu Leu Asn Leu Asn Gly Glu
210 215 220
Leu Ile Gly Ile Asn Thr Ala Ile Leu Ala Pro Gly Gly Gly Ser Val
225 230 235 240
Gly Ile Gly Phe Ala Ile Pro Ser Asn Met Ala Arg Thr Leu Ala Gln
245 250 255
Gln Leu Ile Asp Phe Gly Glu Ile Lys Arg Gly Leu Leu Gly Ile Lys
260 265 270
Gly Thr Glu Met Ser Ala Asp Ile Ala Lys Ala Phe Asn Leu Asp Val
275 280 285
Gln Arg Gly Ala Phe Val Ser Glu Val Leu Pro Gly Ser Gly Ser Ala
290 295 300
Lys Ala Gly Val Lys Ala Gly Asp Ile Ile Thr Ser Leu Asn Gly Lys
305 310 315 320
Pro Leu Asn Ser Phe Ala Glu Leu Arg Ser Arg Ile Ala Thr Thr Glu
325 330 335
Pro Gly Thr Lys Val Lys Leu Gly Leu Leu Arg Asn Gly Lys Pro Leu
340 345 350
Glu Val Glu Val Thr Leu Asp Thr Ser Thr Ser Ser Ser Ala Ser Ala
355 360 365
Glu Met Ile Ala Pro Ala Leu Glu Gly Ala Thr Leu Ser Asp Gly Gln
370 375 380
Leu Lys Asp Gly Gly Lys Gly Ile Lys Ile Asp Glu Val Val Lys Gly
385 390 395 400
Ser Pro Ala Ala Gln Ala Gly Leu Gln Lys Asp Asp Val Ile Ile Gly
405 410 415
Val Asn Arg Asp Arg Val Asn Ser Ile Ala Glu Met Arg Lys Val Leu
420 425 430
Ala Ala Lys Ser Ala Ile Ile Ala Leu Gln Ile Val Arg Gly Asn Glu
435 440 445
Ser Ile Tyr Leu Leu Met Arg
450 455
<210> 124
<211> 396
<212> PRT
<213> 宋内志贺氏菌
<400> 124
Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr
1 5 10 15
Thr Met Met Phe Ser Ala Ser Ala Leu Ala Lys Ile Glu Glu Gly Lys
20 25 30
Leu Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu
35 40 45
Val Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu
50 55 60
His Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly
65 70 75 80
Asp Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr
85 90 95
Ala Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln
100 105 110
Asp Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys
115 120 125
Leu Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn
130 135 140
Lys Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala
145 150 155 160
Leu Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn
165 170 175
Leu Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly
180 185 190
Tyr Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Ala
195 200 205
Val Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu
210 215 220
Ile Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu
225 230 235 240
Ala Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp
245 250 255
Ala Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val
260 265 270
Leu Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu
275 280 285
Ser Ala Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu
290 295 300
Phe Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn
305 310 315 320
Lys Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu
325 330 335
Leu Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys
340 345 350
Gly Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala
355 360 365
Val Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp
370 375 380
Glu Ala Leu Lys Asp Ala Gln Thr Arg Ile Thr Lys
385 390 395
<210> 125
<211> 290
<212> PRT
<213> 鲍氏志贺氏菌
<400> 125
Met Ile Gly Val Thr Asp Gln Lys Gly Glu Lys Ile Leu Leu Asp Thr
1 5 10 15
Thr Gly Pro Ile Ala Arg Thr Asn Thr Ser Tyr Asp Gly Thr Lys Arg
20 25 30
Arg Asn Pro Asn Asn Val Val Asp Leu Lys Asn Arg Lys Tyr Gln Cys
35 40 45
Glu Gln Val Asn Tyr Asp Thr Phe Ile Ser Tyr Pro Gln Leu Asp Ala
50 55 60
Trp Ser Ala His Pro Asp Phe Gln Ser Arg Ile Ser Ala Gln Ile Ala
65 70 75 80
Arg Gln Val Ala Leu Asp Arg Ile Met Ile Gly Phe Asn Gly Thr Ser
85 90 95
His Ala Asp Glu Ser Asn Phe Ser Thr Asn Lys Leu Leu Gln Asp Val
100 105 110
Asn Val Gly Trp Leu Glu His Ile Arg Thr Asp Ala Ser Glu Arg Val
115 120 125
Met Asn Asp Val Thr Leu Thr Ser Arg Asn Met Asp Asn Thr Val Ala
130 135 140
His Ala Gly Lys Tyr Ala Asn Ala Asp Ala Leu Val Gln Asp Ala Arg
145 150 155 160
Ser Ser Leu Leu Asp Glu Trp His Lys Glu Ala Asp Asp Leu Val Val
165 170 175
Ile Met Gly Arg Asn Leu Phe Asn Ser Leu Arg Leu Pro Val Leu Asn
180 185 190
Ser Ile Ser Gly Gln Asn Pro Asn Ala Glu Leu Leu Ala Gly Gln Leu
195 200 205
Ile Leu Ser Ser Arg Thr Ile Gly Gly Leu Gly Val Phe Leu Ala Pro
210 215 220
Phe Phe Pro Asp Ala Thr Met Leu Ile Thr Ser Phe Asn Asn Leu Ser
225 230 235 240
Ile Tyr Trp Gln Lys Gly Ser Met Arg Arg Leu Met Lys Asp Glu Pro
245 250 255
Glu Tyr Asn Arg Ile Ala Thr Tyr Gln Ser Ile Asn Asp Ala Tyr Val
260 265 270
Val Glu Asp Tyr Gly Lys Cys Ala Met Val Thr Gly Leu Lys Phe Ala
275 280 285
Asp Ser
290
<210> 126
<211> 375
<212> PRT
<213> 鲍氏志贺氏菌
<400> 126
Met Thr Ile Asn Phe Cys Arg Asn Ala Leu Gln Leu Ser Val Ala Ala
1 5 10 15
Leu Phe Ser Ser Ala Phe Met Ala Asn Ala Ala Asp Val Pro Gln Val
20 25 30
Lys Val Thr Val Thr Asp Lys Gln Cys Glu Pro Met Thr Ile Thr Val
35 40 45
Asn Ala Gly Lys Thr Gln Phe Ile Ile Gln Asn His Ser Gln Lys Ala
50 55 60
Leu Glu Trp Glu Ile Leu Lys Gly Val Met Val Val Glu Glu Arg Glu
65 70 75 80
Asn Ile Ala Pro Gly Phe Ser Gln Lys Met Thr Ala Asn Leu Gln Pro
85 90 95
Gly Glu Tyr Asp Met Thr Cys Gly Leu Leu Thr Asn Pro Lys Gly Lys
100 105 110
Leu Ile Val Lys Gly Glu Ala Thr Ala Asp Ala Ala Gln Ser Asp Ala
115 120 125
Leu Leu Ser Leu Gly Gly Ala Ile Thr Ala Tyr Lys Ala Tyr Val Met
130 135 140
Ala Glu Thr Thr Gln Leu Val Thr Asp Thr Lys Ala Phe Thr Asp Ala
145 150 155 160
Ile Lys Ala Gly Asp Ile Glu Lys Ala Lys Ala Leu Tyr Ala Pro Thr
165 170 175
Arg Gln His Tyr Glu Arg Ile Glu Pro Ile Ala Glu Leu Phe Ser Asp
180 185 190
Leu Asp Gly Ser Ile Asp Ala Arg Glu Asp Asp Tyr Glu Gln Lys Ala
195 200 205
Ala Asp Pro Lys Phe Thr Gly Phe His Arg Leu Glu Lys Ala Leu Phe
210 215 220
Gly Asp Asn Thr Thr Lys Gly Met Asp Gln Tyr Ala Glu Gln Leu Tyr
225 230 235 240
Thr Asp Val Val Asp Leu Gln Lys Arg Ile Ser Glu Leu Ala Phe Pro
245 250 255
Pro Ser Lys Val Val Gly Gly Ala Ala Gly Leu Ile Glu Glu Val Ala
260 265 270
Ala Ser Lys Ile Ser Gly Glu Glu Asp Arg Tyr Ser His Thr Asp Leu
275 280 285
Trp Asp Phe Gln Ala Asn Val Glu Gly Ser Gln Lys Ile Val Asp Leu
290 295 300
Leu Arg Pro Gln Leu Gln Lys Ala Asn Pro Glu Leu Leu Ala Lys Val
305 310 315 320
Asp Ala Asn Phe Lys Lys Val Asp Thr Ile Leu Ala Lys Tyr Arg Thr
325 330 335
Lys Asp Gly Phe Glu Thr Tyr Asp Lys Leu Thr Asp Ala Asp Arg Asn
340 345 350
Ala Leu Lys Gly Pro Ile Thr Ala Gln Ala Glu Asp Leu Ala Gln Leu
355 360 365
Arg Gly Val Leu Gly Leu Asp
370 375
<210> 127
<211> 236
<212> PRT
<213> 鲍氏志贺氏菌
<400> 127
Met Lys Lys Gly Phe Met Leu Phe Thr Leu Leu Ala Ala Phe Ser Gly
1 5 10 15
Phe Ala Gln Ala Asp Asp Ala Ala Ile Gln Gln Thr Leu Ala Lys Met
20 25 30
Gly Ile Lys Ser Ser Asp Ile Gln Pro Ala Pro Val Ala Gly Met Lys
35 40 45
Thr Val Leu Thr Asn Ser Gly Val Leu Tyr Ile Thr Asp Asp Gly Lys
50 55 60
His Ile Ile Gln Gly Pro Met Tyr Asp Val Ser Gly Ala Ala Pro Val
65 70 75 80
Asn Val Thr Asn Lys Met Leu Leu Lys Gln Leu Asn Ala Leu Glu Lys
85 90 95
Glu Met Ile Val Tyr Lys Ala Pro Gln Glu Lys His Val Ile Thr Val
100 105 110
Phe Thr Asp Ile Thr Cys Gly Tyr Cys His Lys Leu His Glu Gln Met
115 120 125
Ala Asp Tyr Asn Ala Leu Gly Ile Thr Val Arg Tyr Leu Ala Phe Pro
130 135 140
Arg Gln Gly Leu Glu Ser Gln Ala Glu Gln Gln Met Lys Ala Ile Trp
145 150 155 160
Cys Ala Lys Asp Lys Asn Lys Ala Phe Asp Asp Val Met Ala Gly Lys
165 170 175
Ala Ala Thr Pro Ala Ser Cys Asp Ile Asp Ile Ala Asp His Tyr Ala
180 185 190
Leu Gly Val Gln Leu Gly Val Ser Gly Thr Pro Ala Ile Val Leu Ser
195 200 205
Asn Gly Thr Leu Val Pro Gly Tyr Gln Pro Pro Lys Glu Met Lys Glu
210 215 220
Phe Leu Asp Glu His Gln Lys Met Thr Ser Gly Asn
225 230 235
<210> 128
<211> 128
<212> PRT
<213> 痢疾志贺氏菌
<400> 128
Met Ala Gln Gln Phe Tyr Asp Tyr Arg Ile Gln His Arg Ser Asn Asp
1 5 10 15
Ile Thr Ala Leu Arg Pro Tyr Leu Ser Asp Lys Leu Ala Thr Leu Leu
20 25 30
Ser Asp Ala Ser Arg Asp Asn Ser His Arg Glu Leu Leu Ser Ser Asp
35 40 45
Pro Phe Ser Ser Arg Thr Thr Leu Pro Asp Ser Ala His Val Ala Ser
50 55 60
Ala Ser Thr Ile Pro Asn Arg Asp Ala Arg Asn Ile Pro Leu Arg Val
65 70 75 80
Asp Leu Lys Gln Gly Asp Gln Gly Trp Gln Asp Glu Val Leu Met Ile
85 90 95
Gln Glu Gly Gln Cys Trp Val Ile Asp Asp Val Arg Tyr Leu Gly Gly
100 105 110
Ser Val His Ala Thr Ala Gly Thr Leu Arg Gln Ser Ile Glu Asn Arg
115 120 125
<210> 129
<211> 192
<212> PRT
<213> 福氏志贺氏菌
<400> 129
Met Lys Arg Leu Glu His Pro Met Lys Phe Lys Thr Asn Lys Leu Ser
1 5 10 15
Leu Asn Leu Val Leu Ala Ser Ser Leu Leu Ala Ala Ser Ile Pro Ala
20 25 30
Phe Ala Val Thr Gly Asp Thr Asp Gln Pro Ile His Ile Glu Ser Asp
35 40 45
Gln Gln Ser Leu Asp Met Gln Gly Asn Val Val Thr Phe Thr Gly Asn
50 55 60
Val Ile Val Thr Gln Gly Thr Ile Lys Ile Asn Ala Asp Lys Val Val
65 70 75 80
Val Thr Arg Pro Gly Gly Glu Gln Gly Lys Glu Val Ile Asp Gly Tyr
85 90 95
Gly Lys Pro Ala Thr Phe Tyr Gln Met Gln Asp Asn Gly Lys Pro Val
100 105 110
Glu Gly His Ala Ser Gln Met His Tyr Glu Leu Ala Lys Asp Phe Val
115 120 125
Val Leu Thr Gly Asn Ala Tyr Leu Gln Gln Val Asp Ser Asn Ile Lys
130 135 140
Gly Asp Lys Ile Thr Tyr Leu Val Lys Glu Gln Lys Met Gln Ala Phe
145 150 155 160
Ser Asp Lys Gly Lys Arg Val Thr Thr Val Leu Val Pro Ser Gln Leu
165 170 175
Gln Asp Lys Asn Asn Lys Gly Gln Thr Pro Ala Gln Lys Lys Gly Asn
180 185 190
<210> 130
<211> 192
<212> PRT
<213> 福氏志贺氏菌
<400> 130
Met Phe Lys Lys Ile Leu Phe Pro Leu Val Ala Leu Phe Met Leu Ala
1 5 10 15
Gly Cys Ala Lys Pro Pro Thr Thr Ile Glu Val Ser Pro Thr Ile Thr
20 25 30
Leu Pro Gln Gln Asp Pro Ser Leu Met Gly Val Thr Val Ser Ile Asn
35 40 45
Gly Ala Asp Gln Arg Thr Asp Gln Ala Leu Ala Lys Val Thr Arg Asp
50 55 60
Asn Gln Ile Val Thr Leu Thr Ala Ser Arg Ala Leu Arg Phe Leu Leu
65 70 75 80
Gln Glu Val Leu Glu Lys Gln Met Thr Ala Arg Gly Tyr Met Val Gly
85 90 95
Pro Asn Gly Pro Val Asn Leu Gln Ile Ile Val Ser Gln Leu Tyr Ala
100 105 110
Asp Val Ser Gln Gly Asn Val Arg Tyr Asn Ile Ala Thr Lys Ala Asp
115 120 125
Ile Ala Ile Ile Ala Thr Ala Gln Asn Gly Asn Lys Met Thr Lys Asn
130 135 140
Tyr Arg Ala Ser Tyr Asn Val Glu Gly Ala Phe Gln Ala Ser Asn Lys
145 150 155 160
Asn Ile Ala Asp Ala Val Asn Ser Val Leu Thr Asp Thr Ile Ala Asp
165 170 175
Met Ser Gln Asp Thr Ser Ile His Glu Phe Ile Lys Gln Asn Ala Arg
180 185 190
<210> 131
<211> 495
<212> PRT
<213> 鲍氏志贺氏菌
<400> 131
Met Gln Met Lys Lys Leu Leu Pro Ile Leu Ile Gly Leu Ser Leu Ser
1 5 10 15
Gly Phe Ser Ser Leu Ser Gln Ala Glu Asn Leu Met Gln Val Tyr Gln
20 25 30
Gln Ala Arg Leu Ser Asn Pro Glu Leu Arg Lys Ser Ala Ala Asp Arg
35 40 45
Asp Ala Ala Phe Glu Lys Ile Asn Glu Ala Arg Ser Pro Leu Leu Pro
50 55 60
Gln Leu Gly Leu Gly Ala Asp Tyr Thr Tyr Ser Asn Gly Tyr Arg Asp
65 70 75 80
Ala Asn Gly Ile Asn Ser Asn Ala Thr Ser Ala Ser Leu Gln Leu Ala
85 90 95
Gln Ser Ile Phe Asp Met Ser Lys Trp Arg Ala Leu Thr Leu Gln Glu
100 105 110
Lys Ala Ala Gly Ile Gln Asp Val Thr Tyr Gln Thr Asp Gln Gln Thr
115 120 125
Leu Ile Leu Asn Thr Ala Thr Ala Tyr Phe Asn Val Leu Asn Ala Ile
130 135 140
Asp Val Leu Ser Tyr Thr Gln Ala Gln Lys Glu Ala Ile Tyr Arg Gln
145 150 155 160
Leu Asp Gln Thr Thr Gln Arg Phe Asn Val Gly Leu Val Ala Ile Thr
165 170 175
Asp Val Gln Asn Ala Arg Ala Gln Tyr Asp Thr Val Leu Ala Asn Glu
180 185 190
Val Thr Ala Arg Asn Asn Leu Asp Asn Ala Val Glu Gln Leu Arg Gln
195 200 205
Ile Thr Gly Asn Tyr Tyr Pro Glu Leu Ala Ala Leu Asn Val Glu Asn
210 215 220
Phe Lys Thr Asp Lys Pro Gln Pro Val Asn Ala Leu Leu Lys Glu Ala
225 230 235 240
Glu Lys Arg Asn Leu Ser Leu Leu Gln Ala Arg Leu Ser Gln Asp Leu
245 250 255
Ala Arg Glu Gln Ile Arg Gln Ala Gln Asp Gly His Leu Pro Thr Leu
260 265 270
Asp Leu Thr Ala Ser Thr Gly Ile Ser Asp Thr Ser Tyr Ser Gly Ser
275 280 285
Lys Thr Arg Gly Ala Ala Gly Thr Gln Tyr Asp Asp Ser Asn Met Gly
290 295 300
Gln Asn Lys Val Gly Leu Ser Phe Ser Leu Pro Ile Tyr Gln Gly Gly
305 310 315 320
Met Val Asn Ser Gln Val Lys Gln Ala Gln Tyr Asn Phe Val Gly Ala
325 330 335
Ser Glu Gln Leu Glu Ser Ala His Arg Ser Ile Val Gln Thr Val Arg
340 345 350
Ser Ser Phe Asn Asn Ile Asn Ala Ser Ile Ser Ser Ile Asn Ala Tyr
355 360 365
Lys Gln Ala Val Val Ser Ala Gln Ser Ser Leu Asp Ala Met Glu Ala
370 375 380
Gly Tyr Ser Val Gly Thr Arg Thr Ile Val Asp Val Leu Asp Ala Thr
385 390 395 400
Thr Thr Leu Tyr Asn Ala Lys Gln Glu Leu Ala Asn Ala Arg Tyr Asn
405 410 415
Tyr Leu Ile Asn Gln Leu Asn Ile Lys Ser Ala Leu Gly Thr Leu Asn
420 425 430
Glu Gln Asp Leu Leu Ala Leu Asn Asn Ala Leu Ser Lys Pro Val Ser
435 440 445
Thr Asn Pro Glu Asn Val Ala Pro Gln Thr Pro Glu Gln Asn Ala Ile
450 455 460
Ala Asp Gly Tyr Ala Pro Asp Ser Pro Ala Pro Val Val Gln Gln Thr
465 470 475 480
Ser Ala Arg Thr Thr Thr Ser Asn Gly His Asn Pro Phe Arg Asn
485 490 495
<210> 132
<211> 252
<212> PRT
<213> 鲍氏志贺氏菌
<400> 132
Met Lys Ile Arg Ala Leu Leu Val Ala Met Ser Val Ala Thr Val Leu
1 5 10 15
Thr Gly Cys Gln Asn Met Asp Ser Asn Gly Leu Leu Ser Ser Gly Ala
20 25 30
Glu Ala Phe Gln Ala Tyr Ser Leu Ser Asp Ala Gln Val Lys Thr Leu
35 40 45
Ser Asp Gln Ala Cys Gln Glu Met Asp Ser Lys Ala Thr Ile Ala Pro
50 55 60
Ala Asn Ser Glu Tyr Ala Lys Arg Leu Thr Thr Ile Ala Asn Ala Leu
65 70 75 80
Gly Asn Asn Ile Asn Gly Gln Pro Val Asn Tyr Lys Val Tyr Met Ala
85 90 95
Lys Asp Val Asn Ala Phe Ala Met Ala Asn Gly Cys Ile Arg Val Tyr
100 105 110
Ser Gly Leu Met Asp Met Met Thr Asp Asn Glu Val Glu Ala Val Ile
115 120 125
Gly His Glu Met Gly His Val Ala Leu Gly His Val Lys Lys Gly Met
130 135 140
Gln Val Ala Leu Gly Thr Asn Ala Val Arg Val Ala Ala Ala Ser Ala
145 150 155 160
Gly Gly Ile Val Gly Ser Leu Ser Gln Ser Gln Leu Gly Asp Leu Gly
165 170 175
Glu Lys Leu Val Asn Ser Gln Phe Ser Gln Arg Gln Glu Ala Glu Ala
180 185 190
Asp Asp Tyr Ser Tyr Asp Leu Leu Arg Gln Arg Gly Ile Ser Pro Ala
195 200 205
Gly Leu Ala Thr Ser Phe Glu Lys Leu Ala Lys Leu Glu Glu Gly Arg
210 215 220
Gln Ser Ser Met Phe Asp Asp His Pro Ala Ser Ala Glu Arg Ala Gln
225 230 235 240
His Ile Arg Asp Arg Met Ser Ala Asp Gly Ile Lys
245 250
<210> 133
<211> 188
<212> PRT
<213> 鲍氏志贺氏菌
<400> 133
Met Asn Met Thr Lys Gly Ala Leu Ile Leu Ser Leu Ser Phe Leu Leu
1 5 10 15
Ala Ala Cys Ser Ser Ile Pro Gln Asn Ile Lys Gly Asn Asn Gln Pro
20 25 30
Asp Ile Gln Lys Ser Phe Val Ala Val His Asn Gln Pro Gly Leu Tyr
35 40 45
Val Gly Gln Gln Ala Arg Phe Gly Gly Lys Val Ile Asn Val Ile Asn
50 55 60
Gly Lys Thr Asp Thr Leu Leu Glu Ile Ala Val Leu Pro Leu Asp Ser
65 70 75 80
Tyr Ala Lys Pro Asp Ile Glu Ala Asn Tyr Gln Gly Arg Leu Leu Ala
85 90 95
Arg Gln Ser Gly Phe Leu Asp Pro Val Asn Tyr Arg Asn His Phe Val
100 105 110
Thr Ile Leu Gly Thr Ile Gln Gly Glu Gln Pro Gly Phe Ile Asn Lys
115 120 125
Val Pro Tyr Asn Phe Leu Glu Val Asn Met Gln Gly Ile Gln Val Trp
130 135 140
His Leu Arg Glu Val Val Asn Thr Thr Tyr Asn Leu Trp Asp Tyr Gly
145 150 155 160
Tyr Gly Ala Phe Trp Pro Glu Pro Gly Trp Gly Ala Pro Tyr Tyr Thr
165 170 175
Asn Ala Val Ser Gln Val Thr Pro Glu Leu Val Lys
180 185
<210> 134
<211> 201
<212> PRT
<213> 鲍氏志贺氏菌
<400> 134
Met Thr Met Thr Arg Leu Lys Ile Ser Lys Thr Leu Arg Ala Val Met
1 5 10 15
Leu Thr Ser Ala Val Ala Thr Gly Ser Ala Tyr Ala Glu Asn Asn Ala
20 25 30
Gln Thr Thr Asn Glu Ser Ala Gly Gln Lys Val Asp Ser Ser Met Asn
35 40 45
Lys Val Gly Asn Phe Met Asp Asp Ser Ala Ile Thr Ala Lys Val Lys
50 55 60
Ala Ala Leu Val Asp His Asp Asn Ile Lys Ser Thr Asp Ile Ser Val
65 70 75 80
Lys Thr Glu Gln Lys Val Val Thr Leu Ser Gly Phe Val Glu Ser Gln
85 90 95
Ala Gln Ala Glu Glu Ala Val Lys Val Ala Lys Gly Val Glu Gly Val
100 105 110
Thr Ser Val Ser Asp Lys Leu His Val Arg Asp Ala Lys Glu Gly Ser
115 120 125
Val Lys Gly Tyr Ala Gly Asp Thr Ala Thr Thr Ser Glu Ile Lys Ala
130 135 140
Lys Leu Leu Ala Asp Asp Ile Val Pro Ser Arg His Val Lys Val Glu
145 150 155 160
Thr Thr Asp Gly Val Val Gln Leu Ser Gly Thr Val Asp Ser Gln Ala
165 170 175
Gln Ser Asp Arg Ala Glu Ser Ile Ala Lys Ala Val Asp Gly Val Lys
180 185 190
Ser Val Lys Asn Asp Leu Lys Thr Lys
195 200
<210> 135
<211> 765
<212> PRT
<213> 鲍氏志贺氏菌
<400> 135
Met Lys Trp Leu Cys Ser Val Gly Ile Ala Val Ser Leu Ala Leu Gln
1 5 10 15
Pro Ala Leu Ala Asp Asp Leu Phe Gly Asn His Pro Leu Thr Pro Glu
20 25 30
Ala Arg Asp Ala Phe Val Thr Glu Leu Leu Lys Lys Met Thr Val Asp
35 40 45
Glu Lys Ile Gly Gln Leu Arg Leu Ile Ser Val Gly Pro Asp Asn Pro
50 55 60
Lys Glu Ala Ile Arg Glu Met Ile Lys Asp Gly Gln Val Gly Ala Ile
65 70 75 80
Phe Asn Thr Val Thr Arg Gln Asp Ile Arg Ala Met Gln Asp Gln Val
85 90 95
Met Glu Leu Ser Arg Leu Lys Ile Pro Leu Phe Phe Ala Tyr Asp Val
100 105 110
Leu His Gly Gln Arg Thr Val Phe Pro Ile Ser Leu Gly Leu Ala Ser
115 120 125
Ser Phe Asn Leu Asp Ala Val Lys Thr Val Gly Arg Val Ser Ala Tyr
130 135 140
Glu Ala Ala Asp Asp Gly Leu Asn Met Thr Trp Ala Pro Met Val Asp
145 150 155 160
Val Ser Arg Asp Pro Arg Trp Gly Arg Ala Ser Glu Gly Phe Gly Glu
165 170 175
Asp Thr Tyr Leu Thr Ser Thr Met Gly Lys Thr Met Val Glu Ala Met
180 185 190
Gln Gly Lys Ser Pro Ala Asp Arg Tyr Ser Val Met Thr Ser Val Lys
195 200 205
His Phe Ala Ala Tyr Gly Ala Val Glu Gly Gly Lys Glu Tyr Asn Thr
210 215 220
Val Asp Met Ser Pro Gln Arg Leu Phe Asn Asp Tyr Met Pro Pro Tyr
225 230 235 240
Lys Ala Gly Leu Asp Ala Gly Ser Gly Ala Val Met Val Ala Leu Asn
245 250 255
Ser Leu Asn Gly Thr Pro Ala Thr Ser Asp Ser Trp Leu Leu Lys Asp
260 265 270
Val Leu Arg Asp Gln Trp Gly Phe Lys Gly Ile Thr Val Ser Asp His
275 280 285
Gly Ala Ile Lys Glu Leu Ile Lys His Gly Thr Ala Ala Asp Pro Glu
290 295 300
Asp Ala Val Arg Val Ala Leu Lys Ser Gly Ile Asn Met Ser Met Ser
305 310 315 320
Asp Glu Tyr Tyr Ser Lys Tyr Leu Pro Gly Leu Ile Lys Ser Gly Lys
325 330 335
Val Thr Met Ala Glu Leu Asp Asp Ala Ala Arg His Val Leu Asn Val
340 345 350
Lys Tyr Asp Met Gly Leu Phe Asn Asp Pro Tyr Ser His Leu Gly Pro
355 360 365
Lys Glu Ser Asp Pro Val Asp Thr Asn Ala Glu Ser Arg Leu His Arg
370 375 380
Lys Glu Ala Arg Glu Val Ala Arg Glu Ser Leu Val Leu Leu Lys Asn
385 390 395 400
Arg Leu Glu Thr Leu Pro Leu Lys Lys Ser Ala Thr Ile Ala Val Val
405 410 415
Gly Pro Leu Ala Asp Ser Lys Arg Asp Val Met Gly Ser Trp Ser Ala
420 425 430
Ala Gly Val Ala Asp Gln Ser Val Thr Val Leu Thr Gly Ile Lys Asn
435 440 445
Ala Val Gly Glu Asn Gly Lys Val Leu Tyr Ala Lys Gly Ala Asn Val
450 455 460
Thr Ser Asp Lys Gly Ile Ile Asp Phe Leu Asn Gln Tyr Glu Glu Ala
465 470 475 480
Val Lys Val Asp Pro Arg Ser Pro Gln Glu Met Ile Asp Glu Ala Val
485 490 495
Gln Thr Ala Lys Gln Ser Asp Val Val Val Ala Val Val Gly Glu Ala
500 505 510
Gln Gly Met Ala His Glu Ala Ser Ser Arg Thr Asp Ile Thr Ile Pro
515 520 525
Gln Ser Gln Arg Asp Leu Ile Ala Ala Leu Lys Ala Thr Gly Lys Pro
530 535 540
Leu Val Leu Val Leu Met Asn Gly Arg Pro Leu Ala Leu Val Lys Glu
545 550 555 560
Asp Gln Gln Ala Asp Ala Ile Leu Glu Thr Trp Phe Ala Gly Thr Glu
565 570 575
Gly Gly Asn Ala Ile Ala Asp Val Leu Phe Gly Asp Tyr Asn Pro Ser
580 585 590
Gly Lys Leu Pro Met Ser Phe Pro Arg Ser Val Gly Gln Ile Pro Val
595 600 605
Tyr Tyr Ser His Leu Asn Thr Gly Arg Pro Tyr Asn Ala Asp Lys Pro
610 615 620
Asn Lys Tyr Thr Ser Arg Tyr Phe Asp Glu Ala Asn Gly Ala Leu Tyr
625 630 635 640
Pro Phe Gly Tyr Gly Leu Ser Tyr Thr Thr Phe Thr Val Ser Asp Val
645 650 655
Lys Leu Ser Ala Pro Thr Met Lys Arg Asp Gly Lys Val Thr Ala Ser
660 665 670
Val Gln Val Met Asn Thr Gly Lys Arg Glu Gly Ala Thr Val Val Gln
675 680 685
Met Tyr Leu Gln Asp Val Thr Ala Ser Met Ser Arg Pro Val Lys Gln
690 695 700
Leu Lys Gly Phe Glu Lys Ile Thr Leu Lys Pro Gly Glu Thr Gln Thr
705 710 715 720
Val Ser Phe Pro Ile Asp Ile Glu Ala Leu Lys Phe Trp Asn Gln Gln
725 730 735
Met Lys Tyr Asp Ala Glu Pro Gly Lys Phe Asn Val Phe Ile Gly Thr
740 745 750
Asp Ser Ala Arg Val Lys Lys Gly Glu Phe Glu Leu Leu
755 760 765
<210> 136
<211> 503
<212> PRT
<213> 福氏志贺氏菌
<400> 136
Met Glu Phe Ser Val Lys Ser Gly Ser Pro Glu Lys Gln Arg Ser Ala
1 5 10 15
Cys Ile Val Val Gly Val Phe Glu Pro Arg Arg Leu Ser Pro Ile Ala
20 25 30
Glu Gln Leu Asp Lys Ile Ser Asp Gly Tyr Ile Ser Ala Leu Leu Arg
35 40 45
Arg Gly Glu Leu Glu Gly Lys Pro Gly Gln Thr Leu Leu Leu His His
50 55 60
Val Pro Asn Val Leu Ser Glu Arg Ile Leu Leu Ile Gly Cys Gly Lys
65 70 75 80
Glu Arg Glu Leu Asp Glu Arg Gln Tyr Lys Gln Val Ile Gln Lys Thr
85 90 95
Ile Asn Thr Leu Asn Asp Thr Gly Ser Met Glu Ala Val Cys Phe Leu
100 105 110
Thr Glu Leu His Val Lys Gly Arg Asn Asn Tyr Trp Lys Val Arg Gln
115 120 125
Ala Val Glu Thr Ala Lys Glu Thr Leu Tyr Ser Phe Asp Gln Leu Lys
130 135 140
Thr Asn Lys Ser Glu Pro Arg Arg Pro Leu Arg Lys Met Val Phe Asn
145 150 155 160
Val Pro Thr Arg Arg Glu Leu Thr Ser Gly Glu Arg Ala Ile Gln His
165 170 175
Gly Gln Ala Ile Ala Ala Gly Ile Lys Ala Ala Lys Asp Leu Gly Asn
180 185 190
Met Pro Pro Asn Ile Cys Asn Ala Ala Tyr Leu Ala Ser Gln Ala Arg
195 200 205
Gln Leu Ala Asp Ser Tyr Ser Lys Asn Val Ile Thr Arg Val Ile Gly
210 215 220
Glu Gln Gln Met Lys Glu Leu Gly Met His Ser Tyr Leu Ala Val Gly
225 230 235 240
Gln Gly Ser Gln Asn Glu Ser Leu Met Ser Val Ile Glu Tyr Lys Gly
245 250 255
Asn Ala Ser Glu Asp Ala Arg Pro Ile Val Leu Val Gly Lys Gly Leu
260 265 270
Thr Phe Asp Ser Gly Gly Ile Ser Ile Lys Pro Ser Glu Gly Met Asp
275 280 285
Glu Met Lys Tyr Asp Met Cys Gly Ala Ala Ala Val Tyr Gly Val Met
290 295 300
Arg Met Val Ala Glu Leu Gln Leu Pro Ile Asn Val Ile Gly Val Leu
305 310 315 320
Ala Gly Cys Glu Asn Met Pro Gly Gly Arg Ala Tyr Arg Pro Gly Asp
325 330 335
Val Leu Thr Thr Met Ser Gly Gln Thr Val Glu Val Leu Asn Thr Asp
340 345 350
Ala Glu Gly Arg Leu Val Leu Cys Asp Val Leu Thr Tyr Val Glu Arg
355 360 365
Phe Glu Pro Glu Ala Val Ile Asp Val Ala Thr Leu Thr Gly Ala Cys
370 375 380
Val Ile Ala Leu Gly His His Ile Thr Gly Leu Met Thr Asn His Asn
385 390 395 400
Pro Leu Ala His Glu Leu Ile Ala Ala Ser Glu Gln Ser Gly Asp Arg
405 410 415
Ala Trp Arg Leu Pro Leu Gly Asp Glu Tyr Gln Glu Gln Leu Glu Ser
420 425 430
Asn Phe Ala Asp Met Ala Asn Ile Gly Gly Arg Pro Gly Gly Ala Ile
435 440 445
Thr Ala Gly Cys Phe Leu Ser Arg Phe Thr Arg Lys Tyr Asn Trp Ala
450 455 460
His Leu Asp Ile Ala Gly Thr Ala Trp Arg Ser Gly Lys Ala Lys Gly
465 470 475 480
Ala Thr Gly Arg Pro Val Ala Leu Leu Ala Gln Phe Leu Leu Asn Arg
485 490 495
Ala Gly Phe Asn Gly Glu Glu
500

Claims (23)

1.一种志贺氏菌，其表达TolA、TolB、TolQ、TolR和Pal蛋白中的不超过4种蛋白质。
2.如权利要求1所述的细菌，其中所述细菌表达所述蛋白质中的4种，而不表达TolR。
3.一种志贺氏菌，其不表达TolR蛋白。
4.一种细菌，其为志贺氏菌的ΔtolR菌株。
5.如前述任一项权利要求所述的细菌，其中所述细菌不表达天然志贺氏菌的脂多糖。
6.如权利要求5所述的细菌，其中所述细菌不表达天然志贺氏菌O抗原。
7.如权利要求4所述的细菌，其中所述菌株是ΔtolRΔgalU菌株。
8.如前述任一项权利要求所述的细菌，其中stxA和/或stxB基因是失活的。
9.一种表达TolA、TolB、TolQ、TolR和Pal蛋白的志贺氏菌，其中所述TolA、TolQ、TolR和/或Pal蛋白：(a)位于该细菌的内膜或外膜，且(b)包括一个或多个氨基酸序列突变，从而使得与不含所述突变的相同细菌相比，所述细菌在培养基中生长时释放更大量的外膜突泡。
10.一种志贺氏菌，其中其Tol-Pal系统中的一种或多种组分具有修饰，从而使得所述细菌与不含所述修饰的相同细菌相比，所述细菌在培养基中生长的过程中，将更大量的外膜突泡释放到培养基中，且所述细菌不表达：(i)天然志贺氏菌脂多糖和/或(ii)志贺氏菌肠毒素。
11.一种制备志贺氏菌突泡的方法，所述方法包括从包含如权利要求1-10中任一项所述的细菌的培养基中分离所述突泡的步骤，所述细菌已在允许该细菌将突泡释放到所述培养基中的条件下生长。
12.如权利要求11所述的方法，其中所述细菌已在限铁条件下生长。
13.一种制备高起泡性志贺氏菌的方法，所述方法包括如下步骤：对编码起始细菌Tol-Pal系统的一种或多种组分的基因进行修饰，从而使得该修饰导致所述细菌与起始细菌相比，在培养基中生长时，将更大量的外膜突泡释放到培养基中，且其中所述修饰包括使得所述起始细菌的tolA、tolB、tolQ、tolR和/或pal基因中的一种或多种发生突变。
14.一种突泡，其从如权利要求1-10中任一项所述的细菌中分离或可由其获得、或从经如权利要求13所述的方法可获得的细菌中分离或可由其获得、或通过如权利要求11或12所述的方法分离或获得。
15.一种包含突泡的组合物，所述突泡在培养如权利要求1-10中任一项所述的细菌或通过如权利要求13的方法可获得的细菌过程中被释放到培养基中。
16.如权利要求15所述的组合物，其不含任何活菌和/或全菌。
17.一种包含突泡的组合物，其中所述突泡存在于经0.22μm过滤器过滤培养基后可获得的滤出液中，如权利要求1-10中任一项所述的细菌或通过权利要求13所述的方法可获得的细菌已在所述培养基中生长。
18.一种培养基，其包含如权利要求1-10中所述的细菌或通过如权利要求13所述的方法可获得的细菌，所述细菌已在允许所述细菌将突泡释放到所述培养基中的条件下生长。
19.一种志贺氏菌菌株，其不表达功能性HtrB酶。
20.如权利要求19所述的志贺氏菌菌株，其为htrB敲除株。
21.一种志贺氏菌突泡，其包含以下的一种或多种：(a)由选自SEQ ID NO: 8-67的氨基酸序列组成的蛋白质；(b)包含与SEQ ID NO: 8-67之一具有至少85％同一性的氨基酸序列和/或包含SEQ ID NO: 8-67中任一种的至少7个连续氨基酸的片段的蛋白质，其中所述片段包含表位。
22.一种包含蛋白质的无突泡免疫原性组合物，所述蛋白质包含：(a)氨基酸序列SEQID NO: 8-136；(b)氨基酸序列，其与SEQ ID NO: 8-136之一具有至少85％同一性和/或包含SEQ ID NO: 8-136中任一种的至少7个连续氨基酸的片段且包含来自SEQ ID NO: 8-136之一的表位。
23.一种培养志贺氏菌的方法，其中铁可获得性受限制。
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