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NO330802B1 - Metode for gjenvinning av RNA fra formalin-fiksert paraffin-innstopt vevsprove, metode for kvantitativ maling av genekspresjon av et malgen og metode for maling av niva av malgenekspresjon i en fiksert paraffin-innstopt vevsprove. - Google Patents

Metode for gjenvinning av RNA fra formalin-fiksert paraffin-innstopt vevsprove, metode for kvantitativ maling av genekspresjon av et malgen og metode for maling av niva av malgenekspresjon i en fiksert paraffin-innstopt vevsprove. Download PDF

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Publication number
NO330802B1
NO330802B1 NO20022945A NO20022945A NO330802B1 NO 330802 B1 NO330802 B1 NO 330802B1 NO 20022945 A NO20022945 A NO 20022945A NO 20022945 A NO20022945 A NO 20022945A NO 330802 B1 NO330802 B1 NO 330802B1
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rna
sample
expression
approx
tissue sample
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NO20022945A
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English (en)
Norwegian (no)
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NO20022945D0 (no
NO20022945L (no
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Kathleen Dananberg
Peter Dananberg
Steven Swenson
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Univ Southern California
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Publication of NO20022945L publication Critical patent/NO20022945L/no
Publication of NO330802B1 publication Critical patent/NO330802B1/no

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Biophysics (AREA)
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  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
NO20022945A 1999-12-20 2002-06-19 Metode for gjenvinning av RNA fra formalin-fiksert paraffin-innstopt vevsprove, metode for kvantitativ maling av genekspresjon av et malgen og metode for maling av niva av malgenekspresjon i en fiksert paraffin-innstopt vevsprove. NO330802B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/469,338 US6248535B1 (en) 1999-12-20 1999-12-20 Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
PCT/US2000/030012 WO2001046402A1 (en) 1999-12-20 2000-10-31 Method for isolation of rna from formalin-fixed paraffin-embedded tissue specimens

Publications (3)

Publication Number Publication Date
NO20022945D0 NO20022945D0 (no) 2002-06-19
NO20022945L NO20022945L (no) 2002-08-06
NO330802B1 true NO330802B1 (no) 2011-07-18

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NO20022945A NO330802B1 (no) 1999-12-20 2002-06-19 Metode for gjenvinning av RNA fra formalin-fiksert paraffin-innstopt vevsprove, metode for kvantitativ maling av genekspresjon av et malgen og metode for maling av niva av malgenekspresjon i en fiksert paraffin-innstopt vevsprove.

Country Status (20)

Country Link
US (8) US6248535B1 (es)
EP (3) EP1743939B1 (es)
JP (1) JP2003525033A (es)
KR (1) KR100723328B1 (es)
CN (2) CN1269959C (es)
AR (1) AR027036A1 (es)
AT (2) ATE349518T1 (es)
AU (2) AU784565C (es)
BR (1) BR0016569A (es)
CA (1) CA2394618C (es)
DE (1) DE60032634T2 (es)
DK (1) DK1743939T3 (es)
EA (2) EA008832B1 (es)
ES (1) ES2373149T3 (es)
HK (2) HK1055446A1 (es)
IL (2) IL150116A0 (es)
MX (1) MXPA02006145A (es)
NO (1) NO330802B1 (es)
TW (1) TWI277652B (es)
WO (1) WO2001046402A1 (es)

Families Citing this family (218)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU746497B2 (en) * 1997-08-20 2002-05-02 University Of Miami A high quality, continuous throughput, tissue fixation-dehydration-fat removal-impregnation method
US6793890B2 (en) 1997-08-20 2004-09-21 The University Of Miami Rapid tissue processor
US6960439B2 (en) 1999-06-28 2005-11-01 Source Precision Medicine, Inc. Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US20060166227A1 (en) * 2000-06-20 2006-07-27 Stephen Kingsmore Protein expression profiling
US7049059B2 (en) * 2000-12-01 2006-05-23 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression
US20020142328A1 (en) * 2000-12-01 2002-10-03 Danenberg Kathleen D. Method of determining a chemotherapeutic regimen by assaying gene expression in primary tumors
MX2008005896A (es) * 2000-12-01 2009-03-03 Response Genetics Inc Metodo in vitro para predecir la sensibilidad de un tumor a un regimen quimioterapico.
US6582919B2 (en) 2001-06-11 2003-06-24 Response Genetics, Inc. Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates
US6602670B2 (en) * 2000-12-01 2003-08-05 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
US6518416B1 (en) 2000-12-01 2003-02-11 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on ERCC1 expression
US6956111B2 (en) 2001-03-02 2005-10-18 Response Genetics, Inc. Method of determining dihydropyrimidine dehydrogenase gene expression
US6686155B2 (en) * 2001-06-14 2004-02-03 Response Genetics, Inc. Method of determining a chemotherapeutic regimen based on glutathione-S-transferase pi expression
US20040091857A1 (en) * 2001-07-20 2004-05-13 Nallur Girish N. Gene expression profiling
ES2636974T3 (es) * 2001-10-12 2017-10-10 Becton, Dickinson And Company Aparato de tipo cesta para el transporte de muestras biológicas
AU2002340158B8 (en) 2001-10-12 2009-06-11 Becton, Dickinson And Company Method and apparatus for transporting biological samples
CA2466502A1 (en) 2001-11-09 2003-05-15 Source Precision Medicine, Inc. Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
EP2799555B1 (en) * 2002-03-13 2017-02-22 Genomic Health, Inc. Gene expression profiling in biopsied tumor tissues
EP2385139A1 (en) 2002-07-31 2011-11-09 University of Southern California Polymorphisms for predicting disease and treatment outcome
CN100374576C (zh) * 2002-10-11 2008-03-12 阿克丘勒斯生物科学股份有限公司 来自ffpe样品的基因表达分布图
US7364846B2 (en) * 2002-10-11 2008-04-29 Molecular Devices Corporation Gene expression profiling from FFPE samples
EP1572105B1 (en) * 2002-11-14 2011-08-31 John Wayne Cancer Institute Detection of micro metastasis of melanoma and breast cancer in paraffin-embedded tumor draining lymph nodes by multimaker quantitative rt-pcr
US8039218B2 (en) * 2002-11-14 2011-10-18 John Wayne Cancer Institute Detection of cancer cells in body fluids
CA2506066A1 (en) 2002-11-15 2004-06-03 Genomic Health, Inc. Gene expression profiling of egfr positive cancer
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US20040231909A1 (en) 2003-01-15 2004-11-25 Tai-Yang Luh Motorized vehicle having forward and backward differential structure
EP1597391B1 (en) * 2003-02-20 2008-10-29 Genomic Health, Inc. Use of intronic rna to measure gene expression
WO2004080579A2 (en) 2003-03-10 2004-09-23 Expression Pathology, Inc. Liquid tissue preparation from histopatologically processed biological samples, tissues and cells
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
EP1636380A2 (en) * 2003-05-30 2006-03-22 Genomic Health, Inc. Gene expression markers for response to egfr inhibitor drugs
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
JP4680898B2 (ja) 2003-06-24 2011-05-11 ジェノミック ヘルス, インコーポレイテッド 癌再発の可能性の予測
ES2905579T3 (es) * 2003-07-10 2022-04-11 Genomic Health Inc Algoritmo del perfil de expresión y prueba para el pronóstico de la recaída del cáncer de mama
US8062897B2 (en) 2003-07-21 2011-11-22 Aureon Laboratories, Inc. Diagnostic histopathology using multiplex gene expression FISH
WO2005054466A2 (en) * 2003-07-25 2005-06-16 Ambion, Inc. Methods and compositions for preparing rna from a fixed sample
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
WO2005012487A2 (en) * 2003-08-01 2005-02-10 Invitrogen Corporation Compositions and methods for preparing short rna molecules and other nucleic acids
WO2005040396A2 (en) * 2003-10-16 2005-05-06 Genomic Health, Inc. qRT-PCR ASSAY SYSTEM FOR GENE EXPRESSION PROFILING
CA2543043C (en) * 2003-10-24 2013-09-17 The University Of Miami Simplified tissue processing
EP1689858B1 (en) 2003-11-13 2013-05-15 University Of Georgia Research Foundation, Inc. Methods of characterizing infectious bursal disease virus
WO2005064019A2 (en) * 2003-12-23 2005-07-14 Genomic Health, Inc. Universal amplification of fragmented rna
ES2550614T3 (es) 2004-04-09 2015-11-11 Genomic Health, Inc. Marcadores de expresión génica para predecir la respuesta a la quimioterapia
US7794932B2 (en) 2004-04-16 2010-09-14 Piotr Chomczynski Reagents and methods for isolation of purified RNA
US7588890B2 (en) * 2004-04-16 2009-09-15 Wei-Sing Chu Device for extracting biological molecules from tissue specimens and methods for preparing the same
US20050239055A1 (en) * 2004-04-22 2005-10-27 Mariani Brian D Enterovirus primers and probes
US7332597B2 (en) * 2004-06-28 2008-02-19 University Of Kentucky Research Foundation Primers and probe to identify mycobacterium tuberculosis complex
EP1774039A4 (en) 2004-07-01 2009-07-08 Univ Southern California GENETIC MARKERS FOR PREDICTING DISEASES AND THERAPEUTIC RESULTS
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
EP2458015B1 (en) 2004-09-30 2015-12-23 Epigenomics AG Method for providing DNA fragments derived from an archived sample
DE102004047772B4 (de) * 2004-09-30 2018-12-13 Infineon Technologies Ag Lateraler Halbleitertransistor
AU2005304824B2 (en) 2004-11-05 2011-12-22 Genomic Health, Inc. Predicting response to chemotherapy using gene expression markers
ATE550440T1 (de) * 2004-11-05 2012-04-15 Genomic Health Inc Molekulare indikatoren für brustkrebsprognose und vorhersage des ansprechens auf eine behandlung
US20060141481A1 (en) * 2004-12-27 2006-06-29 Mariani Brian D HSV-1 and HSV-2 primers and probes
US7541144B2 (en) * 2005-01-31 2009-06-02 Agilent Technologies, Inc. RNA labeling method
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US7449297B2 (en) * 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
EP2204454A1 (en) * 2005-05-04 2010-07-07 University of South Florida Predicting treatment response in cancer subjects
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
US20060269929A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods and kits for DNA purification on polymeric membranes at low ionic strength
US7544471B2 (en) 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
US20070054168A1 (en) * 2005-09-06 2007-03-08 Hao Chang Zinc/air cell
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP1777291A1 (en) * 2005-10-20 2007-04-25 Fundacion para la Investigacion Clinica y Molecular del Cancer de Pulmon Method for the isolation of mRNA from formalin fixed, paraffin-embedded tissue
US20070092883A1 (en) * 2005-10-26 2007-04-26 De Luwe Hoek Octrooien B.V. Methylation specific multiplex ligation-dependent probe amplification (MS-MLPA)
DE102005060738A1 (de) * 2005-12-16 2007-06-21 Qiagen Gmbh Verfahren zur Extraktion von Biomolekülen aus fixierten Geweben
CN100366632C (zh) * 2005-12-28 2008-02-06 南方医科大学 一种石蜡包埋组织提取蛋白质的方法
CA2667521A1 (en) * 2006-10-27 2008-05-15 George Mason Intellectual Properties, Inc. Assay for metastatic colorectal cancer
ES2365447T3 (es) * 2007-05-03 2011-10-05 Roche Diagnostics Gmbh Cuantificación del arnm de células individuales con rt-pcr en tiempo real.
WO2008157316A1 (en) * 2007-06-14 2008-12-24 Creighton University Improved method for rna isolation
ES2401475T3 (es) * 2007-06-15 2013-04-19 University Of South Florida Métodos de diagnóstico y tratamiento del cáncer
JP2010530761A (ja) 2007-06-22 2010-09-16 レスポンス ジェネティクス,インコーポレイティド 固定されたサンプルからの長い断片rnaの分離方法
AU2008323701B2 (en) 2007-11-07 2015-03-26 Genentech, Inc Methods and compositions for assessing responsiveness of B-cell lymphoma to treatment with anti-CD40 antibodies
EP2262893B1 (en) * 2008-04-15 2013-04-24 Universität Bern Method for optimized isolation of rna from fixed tissue
US20090298172A1 (en) * 2008-05-28 2009-12-03 Steven Paul Wheeler Histological specimen treatment apparatus and method
DE102008029356A1 (de) 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
ES2539856T3 (es) 2008-08-01 2015-07-06 F. Hoffmann-La Roche Ag Lisis mejorada y transcripción reversa para la cuantificación de mRNA
WO2010065775A2 (en) * 2008-12-03 2010-06-10 The United States Government As Represented By The Department Of Veterans Affairs Pressure-assisted molecular recovery (pamr) of biomolecules, pressure-assisted antigen retrieval (paar), and pressure-assisted tissue histology (path)
EP2193831A1 (de) 2008-12-05 2010-06-09 Qiagen GmbH Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe
DE102008061714A1 (de) 2008-12-12 2010-06-17 Siemens Healthcare Diagnostics Inc., Deerfield Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe
US8846878B1 (en) 2009-01-23 2014-09-30 Cubrc Corporation Method and device for isolating a protein sample
EP2210936A1 (en) * 2009-01-27 2010-07-28 Curetis AG Processing and analysis of viscous liquid biological samples
KR101787768B1 (ko) * 2009-04-18 2017-10-18 제넨테크, 인크. 항-cd40 항체를 사용한 치료에 대한 b-세포 림프종의 반응성 평가를 위한 방법
US20120100997A1 (en) 2009-04-24 2012-04-26 University Of Southern California Cd133 polymorphisms and expression predict clinical outcome in patients with cancer
DE102009031434B4 (de) * 2009-07-01 2015-03-05 Axagarius Gmbh & Co. Kg Verwendung eines Kits für die Isolierung von Biomolekülen aus biologischen Proben sowie Kit hierfür
WO2011038019A2 (en) 2009-09-23 2011-03-31 Danisco Us Inc. Novel glycosyl hydrolase enzymes and uses thereof
US8329360B2 (en) 2009-12-04 2012-12-11 Taiwan Semiconductor Manufacturing Company, Ltd. Method and apparatus of providing overlay
UA114276C2 (uk) 2009-12-23 2017-05-25 Даніско Юес Інк. Спосіб одночасного оцукрювання і ферментації
CN102803490B (zh) * 2010-01-04 2015-03-25 奇亚根盖瑟斯堡股份有限公司 用于从固定组织样品回收核酸或蛋白质的方法、组合物和试剂盒
WO2011104027A1 (en) * 2010-02-26 2011-09-01 Qiagen Gmbh Process for parallel isolation and/or purification of rna and dna
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
EP2556158B1 (en) 2010-04-08 2019-12-18 Qiagen GmbH Chromatographic device and method for isolating and purifying nucleic acids
US9163228B2 (en) 2010-04-08 2015-10-20 Qiagen Gmbh Method for isolating and purifying nucleic acids
CN102869790B (zh) 2010-04-12 2014-11-26 反应遗传有限公司 Kras引物和探针
EP2388324A1 (de) * 2010-05-20 2011-11-23 Siemens Healthcare Diagnostics Inc. Kontrollmaterial für Nukleinsäureteste
EP2395082A1 (en) * 2010-06-14 2011-12-14 QIAGEN GmbH Extraction of nucleic acids from wax-embedded samples
CN103261873A (zh) * 2010-10-12 2013-08-21 生命科技股份有限公司 制备用于ffpe的质量对照材料的方法
KR101420094B1 (ko) * 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량pcr을 이용한 병원균의 전자동 생균수검사방법, 정량면역pcr을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
TWI425977B (zh) 2010-11-24 2014-02-11 Ind Tech Res Inst 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組
EP2468862A1 (en) * 2010-12-23 2012-06-27 Qiagen GmbH Method and kit for processing wax-embedded biological samples
CN102146112B (zh) * 2011-01-25 2013-07-10 天根生化科技(北京)有限公司 从福尔马林固定石蜡包埋组织中提取脱氧核糖核酸的方法
CA2826056C (en) * 2011-02-28 2021-01-26 Dako Denmark A/S Two phase immiscible system for the pretreatment of embedded biological samples
CN102181431B (zh) * 2011-03-11 2012-12-26 大连医科大学 从石蜡包埋组织中提取总rna的方法
BR112013023757A2 (pt) 2011-03-17 2017-06-06 Danisco Us Inc método para redução de viscosidade no processo de sacarificação
WO2012135125A1 (en) * 2011-03-28 2012-10-04 Geron Corporation Telomere length measurement in formalin-fixed, paraffin embedded (ffpe) samples by quantitative pcr
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2744916A4 (en) 2011-07-13 2015-06-17 Primeradx Inc MULTIMODAL METHODS FOR SIMULTANEOUS DETECTION AND QUANTIFICATION OF MULTIPLE NUCLEIC ACIDS IN A SAMPLE
FR2982278A1 (fr) 2011-11-03 2013-05-10 Assist Publ Hopitaux De Paris Procede de classification d'echantillons de tissus fixes et inclus en paraffine
US9416357B2 (en) 2011-11-16 2016-08-16 University Of Georgia Research Foundation, Inc. Method for isolating total RNA from cells
WO2013074885A1 (en) 2011-11-17 2013-05-23 Rheonix, Inc. Microfluidic apparatus, method, and applications
CN103571824B (zh) 2012-08-09 2016-04-13 财团法人工业技术研究院 用于分离核酸的组合物及方法
EP2900819B1 (en) 2012-09-28 2020-10-28 Cepheid Methods for dna and rna extraction from fixed paraffin-embedded tissue samples
EP2906703A2 (en) 2012-10-15 2015-08-19 Pioneer Hi-Bred International, Inc. Methods and compositions to enhance activity of cry endotoxins
DK3511423T4 (da) 2012-10-17 2024-07-29 Spatial Transcriptomics Ab Fremgangsmåder og produkt til optimering af lokaliseret eller rumlig detektion af genekspression i en vævsprøve
US10968446B2 (en) 2012-11-01 2021-04-06 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
EP2730653A1 (en) 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample
US9200327B2 (en) 2012-11-30 2015-12-01 Geron Corporation Diagnostic markers for treating cell proliferative disorders with telomerase inhibitors
EP2970363B1 (en) 2013-03-14 2020-07-08 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US10023877B2 (en) 2013-03-15 2018-07-17 Pioneer Hi-Bred International, Inc. PHI-4 polypeptides and methods for their use
KR101748867B1 (ko) * 2013-04-19 2017-06-20 주식회사 젠큐릭스 조기 유방암 예후 예측 진단용 자동화 시스템
CA2916660C (en) 2013-06-25 2022-05-17 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
EP3032942B1 (en) 2013-08-16 2020-03-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX359027B (es) 2013-09-13 2018-09-12 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
CN114763376A (zh) 2014-02-07 2022-07-19 先锋国际良种公司 杀昆虫蛋白及其使用方法
RU2747978C2 (ru) 2014-02-07 2021-05-18 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
WO2016000237A1 (en) 2014-07-03 2016-01-07 Pioneer Overseas Corporation Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes
WO2016044092A1 (en) 2014-09-17 2016-03-24 Pioneer Hi Bred International Inc Compositions and methods to control insect pests
MX2017004745A (es) 2014-10-16 2017-10-20 Pioner Hi-Bred Int Inc Proteinas insecticidas y metodos de uso.
DE102014118532B4 (de) 2014-12-12 2017-03-23 Axagarius Gmbh & Co. Kg Vorrichtung mit einer Filterschicht und Verfahren zur Gewinnung von Nukleinsäuren aus Formalin-fixierten und in Paraffin eingebetteten Proben
US10968443B2 (en) * 2014-12-31 2021-04-06 The Rockefeller University Method of RNA isolation from clinical samples
WO2016144688A1 (en) 2015-03-11 2016-09-15 Pioneer Hi Bred International Inc Insecticidal combinations of pip-72 and methods of use
JP6828007B2 (ja) 2015-04-10 2021-02-10 スペーシャル トランスクリプトミクス アクチボラグ 生物学的試料の空間識別されるマルチプレックスな核酸分析
US10365189B2 (en) 2015-05-07 2019-07-30 Steven Wheeler Histological specimen treatment
CN116333064A (zh) 2015-05-19 2023-06-27 先锋国际良种公司 杀昆虫蛋白及其使用方法
EA033170B1 (ru) * 2015-05-21 2019-09-30 Государственное Учреждение Образования "Белорусская Медицинская Академия Последипломного Образования" (Гуо "Белмапо") Способ определения экспрессии гена gstm1 в образцах из парафин-фиксированных срезов опухолевой ткани молочной железы
MX2017016119A (es) 2015-06-16 2018-03-07 Pioneer Hi Bred Int Composiciones y metodos para controlar plagas de insectos.
KR20230152796A (ko) 2015-07-13 2023-11-03 피벗 바이오, 인크. 식물 형질 개선을 위한 방법 및 조성물
EP3705582A1 (en) 2015-07-24 2020-09-09 Cepheid Compositions and methods for dna and rna extraction from tissue samples
WO2017023486A1 (en) 2015-08-06 2017-02-09 Pioneer Hi-Bred International, Inc. Plant derived insecticidal proteins and methods for their use
ES2773072T3 (es) 2015-08-28 2020-07-09 Pioneer Hi Bred Int Transformación de plantas mediada por Ochrobactrum
US11479516B2 (en) 2015-10-05 2022-10-25 Massachusetts Institute Of Technology Nitrogen fixation using refactored NIF clusters
KR101749419B1 (ko) 2015-10-15 2017-07-03 주식회사 어큐진 파라핀포매된 샘플로부터 dna를 추출하는 방법
CN108449995B (zh) 2015-11-06 2022-02-01 文塔纳医疗系统公司 代表性诊断
EP3390431A1 (en) 2015-12-18 2018-10-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
BR112018070754A2 (pt) 2016-04-14 2019-02-12 Pioneer Hi-Bred International, Inc. polipeptídeos, polinucleotídeos, construção de dna, célula hospedeira, plantas ou célula vegetal, semente, composição, método para controlar uma população de pragas lepidópteras, método para exterminar uma praga de lepidóptero, método para produzir um polipeptídeo com atividade pesticida e método para proteger uma planta contra uma praga de inseto
EP3445861B1 (en) 2016-04-19 2021-12-08 Pioneer Hi-Bred International, Inc. Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof
EP3451837B1 (en) 2016-05-04 2021-08-25 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2017218207A1 (en) 2016-06-16 2017-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CN116334123A (zh) 2016-06-24 2023-06-27 先锋国际良种公司 植物调节元件及其使用方法
MX2018015906A (es) 2016-07-01 2019-04-04 Pioneer Hi Bred Int Proteinas insecticidas de plantas y metodos para sus usos.
WO2018013333A1 (en) 2016-07-12 2018-01-18 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CN109863167B (zh) 2016-11-01 2022-12-23 先锋国际良种公司 杀昆虫蛋白及其使用方法
WO2018111551A1 (en) 2016-12-14 2018-06-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3046226A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3559271A1 (en) 2016-12-22 2019-10-30 Ventana Medical Systems, Inc. Fully automated nucleic acid extraction method for tissue samples
KR102698684B1 (ko) 2017-01-12 2024-08-27 피벗 바이오, 인크. 식물 형질 개선을 위한 방법 및 조성물
WO2018140214A1 (en) 2017-01-24 2018-08-02 Pioneer Hi-Bred International, Inc. Nematicidal protein from pseudomonas
MX2019009371A (es) 2017-02-08 2019-09-23 Pionner Hi Bred Int Inc Combinaciones insecticidas de proteinas insecticidas derivadas de plantas y metodos para su uso.
EA201992824A1 (ru) 2017-05-26 2020-05-06 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные полипептиды, обладающие улучшенным спектром активности, и пути их применения
CN107271239A (zh) * 2017-07-06 2017-10-20 浙江大学 一种溶解石蜡组织切片的方法及应用
WO2019074598A1 (en) 2017-10-13 2019-04-18 Pioneer Hi-Bred International, Inc. VIRUS-INDUCED GENETIC SILENCING TECHNOLOGY FOR THE CONTROL OF INSECTS IN MAIZE
EP3701040A4 (en) 2017-10-25 2021-08-25 Pivot Bio, Inc. METHODS AND COMPOSITIONS FOR IMPROVING GENETICALLY MODIFIED MICROBES THAT BIND NITROGEN
KR20200123144A (ko) 2018-02-22 2020-10-28 지머젠 인코포레이티드 바실러스가 농축된 게놈 라이브러리를 생성하고 새로운 cry 독소를 동정하기 위한 방법
WO2019169150A1 (en) 2018-03-02 2019-09-06 Pioneer Hi-Bred International, Inc. Plant health assay
BR112020017975A2 (pt) 2018-03-02 2020-12-29 Zymergen Inc. Plataforma de descoberta de proteína inseticida e proteínas inseticidas descobertas a partir da mesma
MX2020009435A (es) 2018-03-14 2020-11-11 Pioneer Hi Bred Int Proteinas con actividad insecticida de plantas y metodos para sus usos.
WO2019178038A1 (en) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US11702668B2 (en) 2018-05-22 2023-07-18 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
CA3103977A1 (en) 2018-06-27 2020-01-02 Pivot Bio, Inc. Agricultural compositions comprising remodeled nitrogen fixing microbes
MX2021002290A (es) 2018-08-29 2021-04-28 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
US20220049294A1 (en) 2018-12-10 2022-02-17 10X Genomics, Inc. Imaging system hardware
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN110376036A (zh) * 2019-07-30 2019-10-25 河南赛诺特生物技术有限公司 一种肿瘤细胞系免疫组化对照品的制备方法及肿瘤细胞系免疫组化对照品
CN110361242B (zh) * 2019-08-14 2022-01-18 武汉赛维尔生物科技有限公司 一种用于眼球组织的固定液以及眼球组织制片的预处理方法
WO2021076346A1 (en) 2019-10-18 2021-04-22 Pioneer Hi-Bred International, Inc. Maize event dp-202216-6 and dp-023211-2 stack
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021133845A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
SG11202106899SA (en) 2019-12-23 2021-09-29 10X Genomics Inc Methods for spatial analysis using rna-templated ligation
US20210190770A1 (en) 2019-12-23 2021-06-24 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
TW202142114A (zh) 2020-02-04 2021-11-16 美商陶氏農業科學公司 具有殺有害生物效用之組成物及與其相關之方法
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
JP2023521579A (ja) 2020-03-31 2023-05-25 キアゲン ゲーエムベーハー 固定された生体試料からの核酸精製
WO2021207610A1 (en) 2020-04-10 2021-10-14 10X Genomics, Inc. Cold protease treatment method for preparing biological samples
CN115916972A (zh) 2020-04-16 2023-04-04 10X基因组学有限公司 用于被固定样品的组合物和方法
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
EP4153776A1 (en) 2020-05-22 2023-03-29 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4414459A3 (en) 2020-05-22 2024-09-18 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
ES2981265T3 (es) 2020-06-08 2024-10-08 10X Genomics Inc Métodos para determinar un margen quirúrgico y métodos de uso del mismo
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
EP4182466A2 (en) 2020-07-14 2023-05-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN116323968A (zh) 2020-07-31 2023-06-23 10X基因组学有限公司 用于空间分析的去交联化合物和使用方法
WO2022035649A1 (en) 2020-08-10 2022-02-17 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
EP4244379A1 (en) 2020-11-13 2023-09-20 10X Genomics, Inc. Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
CN114854824A (zh) * 2021-02-03 2022-08-05 深圳华大基因股份有限公司 建库方法及应用
EP4347879A1 (en) 2021-06-03 2024-04-10 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US20230071935A1 (en) * 2021-08-25 2023-03-09 Matchstick Technologies, Inc. Microplate cycling thermo sonicator with programmable ultrasound, heating, and cooling for multi-analytical applications
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
TW202345696A (zh) 2022-05-18 2023-12-01 美商科迪華農業科技有限責任公司 具有殺有害生物效用之組成物及與其相關的方法
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4830969A (en) 1981-08-31 1989-05-16 The Research Foundation Of State University Of New York Process for the rapid and simple isolation of nucleic acids
US4843155A (en) 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5128247A (en) 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5284940A (en) 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
US5620852A (en) 1990-11-14 1997-04-15 Hri Research, Inc. Nucleic acid preparation methods
US5654179A (en) * 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
US5346994A (en) * 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5502166A (en) 1992-02-26 1996-03-26 Mitsubishi Chemical Corporation NMDH receptor proteins and genes encoding the same
AU6230594A (en) 1993-02-01 1994-08-29 University Of Iowa Research Foundation, The Quartenary amine surfactants and methods of using same in isolation of rna
US5876978A (en) * 1993-04-06 1999-03-02 Medical College Of Ohio Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5637687A (en) 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
US5643767A (en) 1994-05-02 1997-07-01 The Rockefeller University Process for isolating cellular components
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
CA2153215A1 (en) 1994-07-06 1996-01-07 Lu Wang Treatment of paraffin embedded tissue for gene analysis
US5763169A (en) * 1995-01-13 1998-06-09 Chiron Diagnostics Corporation Nucleic acid probes for the detection and identification of fungi
US5707802A (en) * 1995-01-13 1998-01-13 Ciba Corning Diagnostics Corp. Nucleic acid probes for the detection and identification of fungi
US5777099A (en) 1995-02-24 1998-07-07 Biotecx Laboratories, Inc. RNA separation
US5945515A (en) 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US5998151A (en) 1995-12-01 1999-12-07 The United States Of America As Represented By The Department Of Health And Human Services Methods for predicting the efficacy of a chemotherapeutic regimen for gastrointestinal cancers using antibodies specific for thymidylate synthase
US20020090642A1 (en) * 1995-12-11 2002-07-11 Patrick G. Johnston Methods for determining the prognosis of breast cancer using antibodies specific for thymidylate synthase
DE29601618U1 (de) 1996-01-31 1996-07-04 InViTek GmbH, 13125 Berlin Vorrichtung zur gleichzeitigen multiplen Isolierung
EP0791654A1 (en) 1996-02-21 1997-08-27 Jürgen A. Dr. Richt Polypeptides corresponding to the amino acid sequences of proteins p57 or p9.5 of Borna disease virus, nucleic acid fragments coding therefore and their use for diagnostic and immunization purposes
EP0912726A4 (en) * 1996-05-10 2001-07-18 Phylomed Corp METHODS FOR OXIDIZING BISULFIDE LINKS WITH OZONE
CA2284686A1 (en) * 1997-03-31 1998-10-08 Abbott Laboratories Reagents and methods useful for detecting diseases of the gastrointestinal tract
CA2366231C (en) * 1999-03-18 2010-05-11 Exiqon A/S One step sample preparation and detection of nucleic acids in complex biological samples
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens

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