NO330802B1 - Metode for gjenvinning av RNA fra formalin-fiksert paraffin-innstopt vevsprove, metode for kvantitativ maling av genekspresjon av et malgen og metode for maling av niva av malgenekspresjon i en fiksert paraffin-innstopt vevsprove. - Google Patents
Metode for gjenvinning av RNA fra formalin-fiksert paraffin-innstopt vevsprove, metode for kvantitativ maling av genekspresjon av et malgen og metode for maling av niva av malgenekspresjon i en fiksert paraffin-innstopt vevsprove. Download PDFInfo
- Publication number
- NO330802B1 NO330802B1 NO20022945A NO20022945A NO330802B1 NO 330802 B1 NO330802 B1 NO 330802B1 NO 20022945 A NO20022945 A NO 20022945A NO 20022945 A NO20022945 A NO 20022945A NO 330802 B1 NO330802 B1 NO 330802B1
- Authority
- NO
- Norway
- Prior art keywords
- rna
- sample
- expression
- approx
- tissue sample
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 93
- 230000014509 gene expression Effects 0.000 title claims description 61
- 238000005259 measurement Methods 0.000 title claims description 4
- 101150108610 MAL gene Proteins 0.000 title 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 83
- 108010022394 Threonine synthase Proteins 0.000 claims description 58
- 102000005497 Thymidylate Synthase Human genes 0.000 claims description 57
- 239000000523 sample Substances 0.000 claims description 49
- 108090000623 proteins and genes Proteins 0.000 claims description 31
- 230000003196 chaotropic effect Effects 0.000 claims description 30
- 238000010438 heat treatment Methods 0.000 claims description 24
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 claims description 19
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 16
- 238000010839 reverse transcription Methods 0.000 claims description 16
- 239000002299 complementary DNA Substances 0.000 claims description 14
- 238000011084 recovery Methods 0.000 claims description 13
- 239000003960 organic solvent Substances 0.000 claims description 12
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 108020004999 messenger RNA Proteins 0.000 claims description 7
- 238000000605 extraction Methods 0.000 claims description 6
- 238000000746 purification Methods 0.000 claims description 6
- 230000003321 amplification Effects 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 5
- 238000004587 chromatography analysis Methods 0.000 claims description 5
- 238000001962 electrophoresis Methods 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- 238000003752 polymerase chain reaction Methods 0.000 claims description 5
- 239000003638 chemical reducing agent Substances 0.000 claims description 4
- 238000000265 homogenisation Methods 0.000 claims description 4
- 238000013467 fragmentation Methods 0.000 claims description 3
- 238000006062 fragmentation reaction Methods 0.000 claims description 3
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims description 3
- -1 guanidinium compound Chemical class 0.000 claims description 3
- 108020004707 nucleic acids Proteins 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims description 3
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims description 2
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 claims description 2
- 239000002751 oligonucleotide probe Substances 0.000 claims description 2
- 238000012869 ethanol precipitation Methods 0.000 claims 1
- 238000010208 microarray analysis Methods 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 description 62
- 239000000243 solution Substances 0.000 description 39
- 206010028980 Neoplasm Diseases 0.000 description 29
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 28
- 239000012188 paraffin wax Substances 0.000 description 20
- 239000008188 pellet Substances 0.000 description 14
- GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 description 12
- 238000010240 RT-PCR analysis Methods 0.000 description 12
- 239000003795 chemical substances by application Substances 0.000 description 12
- 229960002949 fluorouracil Drugs 0.000 description 12
- 108020004414 DNA Proteins 0.000 description 11
- 230000004044 response Effects 0.000 description 11
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 10
- 206010009944 Colon cancer Diseases 0.000 description 9
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 9
- 101150112897 TS gene Proteins 0.000 description 9
- 108010085238 Actins Proteins 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 102000007469 Actins Human genes 0.000 description 7
- 206010027476 Metastases Diseases 0.000 description 7
- 238000005119 centrifugation Methods 0.000 description 7
- 238000002560 therapeutic procedure Methods 0.000 description 7
- 108010037462 Cyclooxygenase 2 Proteins 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
- 102100038280 Prostaglandin G/H synthase 2 Human genes 0.000 description 6
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 6
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 6
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 6
- 210000001072 colon Anatomy 0.000 description 6
- 208000029742 colonic neoplasm Diseases 0.000 description 6
- 238000002955 isolation Methods 0.000 description 6
- 230000009401 metastasis Effects 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 6
- 238000003753 real-time PCR Methods 0.000 description 6
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 239000008096 xylene Substances 0.000 description 6
- 102100034343 Integrase Human genes 0.000 description 5
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 5
- 101710132082 Pyrimidine/purine nucleoside phosphorylase Proteins 0.000 description 5
- 238000002123 RNA extraction Methods 0.000 description 5
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 5
- 102000013537 Thymidine Phosphorylase Human genes 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 239000008346 aqueous phase Substances 0.000 description 5
- 239000012472 biological sample Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- VVIAGPKUTFNRDU-UHFFFAOYSA-N 6S-folinic acid Natural products C1NC=2NC(N)=NC(=O)C=2N(C=O)C1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 VVIAGPKUTFNRDU-UHFFFAOYSA-N 0.000 description 4
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 4
- 150000001298 alcohols Chemical class 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 4
- 210000003238 esophagus Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- VVIAGPKUTFNRDU-ABLWVSNPSA-N folinic acid Chemical compound C1NC=2NC(N)=NC(=O)C=2N(C=O)C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 VVIAGPKUTFNRDU-ABLWVSNPSA-N 0.000 description 4
- 235000008191 folinic acid Nutrition 0.000 description 4
- 239000011672 folinic acid Substances 0.000 description 4
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 4
- 230000016507 interphase Effects 0.000 description 4
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 4
- 229960001691 leucovorin Drugs 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000012074 organic phase Substances 0.000 description 4
- 239000012071 phase Substances 0.000 description 4
- 238000011002 quantification Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 229920002527 Glycogen Polymers 0.000 description 3
- 108010077895 Sarcosine Proteins 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 229940096919 glycogen Drugs 0.000 description 3
- ZRALSGWEFCBTJO-UHFFFAOYSA-O guanidinium Chemical compound NC(N)=[NH2+] ZRALSGWEFCBTJO-UHFFFAOYSA-O 0.000 description 3
- 238000004255 ion exchange chromatography Methods 0.000 description 3
- 206010061289 metastatic neoplasm Diseases 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 238000002205 phenol-chloroform extraction Methods 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 229940043230 sarcosine Drugs 0.000 description 3
- 238000001542 size-exclusion chromatography Methods 0.000 description 3
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 2
- 208000026310 Breast neoplasm Diseases 0.000 description 2
- 102100022334 Dihydropyrimidine dehydrogenase [NADP(+)] Human genes 0.000 description 2
- 108010066455 Dihydrouracil Dehydrogenase (NADP) Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- YNQLUTRBYVCPMQ-UHFFFAOYSA-N Ethylbenzene Chemical compound CCC1=CC=CC=C1 YNQLUTRBYVCPMQ-UHFFFAOYSA-N 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical class NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 239000002246 antineoplastic agent Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 229940127089 cytotoxic agent Drugs 0.000 description 2
- GYOZYWVXFNDGLU-XLPZGREQSA-N dTMP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 GYOZYWVXFNDGLU-XLPZGREQSA-N 0.000 description 2
- JSRLJPSBLDHEIO-SHYZEUOFSA-N dUMP Chemical compound O1[C@H](COP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 JSRLJPSBLDHEIO-SHYZEUOFSA-N 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 230000002055 immunohistochemical effect Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 2
- 238000010898 silica gel chromatography Methods 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- WAVYAFBQOXCGSZ-UHFFFAOYSA-N 2-fluoropyrimidine Chemical compound FC1=NC=CC=N1 WAVYAFBQOXCGSZ-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 101150071146 COX2 gene Proteins 0.000 description 1
- 101100114534 Caenorhabditis elegans ctc-2 gene Proteins 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 208000000461 Esophageal Neoplasms Diseases 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical class CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 206010030155 Oesophageal carcinoma Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 101150000187 PTGS2 gene Proteins 0.000 description 1
- 108010010677 Phosphodiesterase I Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 229940044683 chemotherapy drug Drugs 0.000 description 1
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000002254 cytotoxic agent Substances 0.000 description 1
- 231100000599 cytotoxic agent Toxicity 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000003989 endothelium vascular Anatomy 0.000 description 1
- 201000004101 esophageal cancer Diseases 0.000 description 1
- 239000004052 folic acid antagonist Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 150000002357 guanidines Chemical class 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 208000014829 head and neck neoplasm Diseases 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- UWKQSNNFCGGAFS-XIFFEERXSA-N irinotecan Chemical compound C1=C2C(CC)=C3CN(C(C4=C([C@@](C(=O)OC4)(O)CC)C=4)=O)C=4C3=NC2=CC=C1OC(=O)N(CC1)CCC1N1CCCCC1 UWKQSNNFCGGAFS-XIFFEERXSA-N 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 208000026037 malignant tumor of neck Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 210000003739 neck Anatomy 0.000 description 1
- 208000025402 neoplasm of esophagus Diseases 0.000 description 1
- 210000005170 neoplastic cell Anatomy 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 230000002974 pharmacogenomic effect Effects 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000007692 polyacrylamide-agarose gel electrophoresis Methods 0.000 description 1
- ZNNZYHKDIALBAK-UHFFFAOYSA-M potassium thiocyanate Chemical compound [K+].[S-]C#N ZNNZYHKDIALBAK-UHFFFAOYSA-M 0.000 description 1
- 229940116357 potassium thiocyanate Drugs 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical class CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 239000002719 pyrimidine nucleotide Substances 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
- 238000011155 quantitative monitoring Methods 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000006485 reductive methylation reaction Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 208000037921 secondary disease Diseases 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 239000000439 tumor marker Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 150000003738 xylenes Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/469,338 US6248535B1 (en) | 1999-12-20 | 1999-12-20 | Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens |
PCT/US2000/030012 WO2001046402A1 (en) | 1999-12-20 | 2000-10-31 | Method for isolation of rna from formalin-fixed paraffin-embedded tissue specimens |
Publications (3)
Publication Number | Publication Date |
---|---|
NO20022945D0 NO20022945D0 (no) | 2002-06-19 |
NO20022945L NO20022945L (no) | 2002-08-06 |
NO330802B1 true NO330802B1 (no) | 2011-07-18 |
Family
ID=23863395
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO20022945A NO330802B1 (no) | 1999-12-20 | 2002-06-19 | Metode for gjenvinning av RNA fra formalin-fiksert paraffin-innstopt vevsprove, metode for kvantitativ maling av genekspresjon av et malgen og metode for maling av niva av malgenekspresjon i en fiksert paraffin-innstopt vevsprove. |
Country Status (20)
Country | Link |
---|---|
US (8) | US6248535B1 (es) |
EP (3) | EP1743939B1 (es) |
JP (1) | JP2003525033A (es) |
KR (1) | KR100723328B1 (es) |
CN (2) | CN1269959C (es) |
AR (1) | AR027036A1 (es) |
AT (2) | ATE349518T1 (es) |
AU (2) | AU784565C (es) |
BR (1) | BR0016569A (es) |
CA (1) | CA2394618C (es) |
DE (1) | DE60032634T2 (es) |
DK (1) | DK1743939T3 (es) |
EA (2) | EA008832B1 (es) |
ES (1) | ES2373149T3 (es) |
HK (2) | HK1055446A1 (es) |
IL (2) | IL150116A0 (es) |
MX (1) | MXPA02006145A (es) |
NO (1) | NO330802B1 (es) |
TW (1) | TWI277652B (es) |
WO (1) | WO2001046402A1 (es) |
Families Citing this family (218)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU746497B2 (en) * | 1997-08-20 | 2002-05-02 | University Of Miami | A high quality, continuous throughput, tissue fixation-dehydration-fat removal-impregnation method |
US6793890B2 (en) | 1997-08-20 | 2004-09-21 | The University Of Miami | Rapid tissue processor |
US6960439B2 (en) | 1999-06-28 | 2005-11-01 | Source Precision Medicine, Inc. | Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles |
US6248535B1 (en) * | 1999-12-20 | 2001-06-19 | University Of Southern California | Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens |
US20060166227A1 (en) * | 2000-06-20 | 2006-07-27 | Stephen Kingsmore | Protein expression profiling |
US7049059B2 (en) * | 2000-12-01 | 2006-05-23 | Response Genetics, Inc. | Method of determining a chemotherapeutic regimen based on ERCC1 and TS expression |
US20020142328A1 (en) * | 2000-12-01 | 2002-10-03 | Danenberg Kathleen D. | Method of determining a chemotherapeutic regimen by assaying gene expression in primary tumors |
MX2008005896A (es) * | 2000-12-01 | 2009-03-03 | Response Genetics Inc | Metodo in vitro para predecir la sensibilidad de un tumor a un regimen quimioterapico. |
US6582919B2 (en) | 2001-06-11 | 2003-06-24 | Response Genetics, Inc. | Method of determining epidermal growth factor receptor and HER2-neu gene expression and correlation of levels thereof with survival rates |
US6602670B2 (en) * | 2000-12-01 | 2003-08-05 | Response Genetics, Inc. | Method of determining a chemotherapeutic regimen based on ERCC1 expression |
US6518416B1 (en) | 2000-12-01 | 2003-02-11 | Response Genetics, Inc. | Method of determining a chemotherapeutic regimen based on ERCC1 expression |
US6956111B2 (en) | 2001-03-02 | 2005-10-18 | Response Genetics, Inc. | Method of determining dihydropyrimidine dehydrogenase gene expression |
US6686155B2 (en) * | 2001-06-14 | 2004-02-03 | Response Genetics, Inc. | Method of determining a chemotherapeutic regimen based on glutathione-S-transferase pi expression |
US20040091857A1 (en) * | 2001-07-20 | 2004-05-13 | Nallur Girish N. | Gene expression profiling |
ES2636974T3 (es) * | 2001-10-12 | 2017-10-10 | Becton, Dickinson And Company | Aparato de tipo cesta para el transporte de muestras biológicas |
AU2002340158B8 (en) | 2001-10-12 | 2009-06-11 | Becton, Dickinson And Company | Method and apparatus for transporting biological samples |
CA2466502A1 (en) | 2001-11-09 | 2003-05-15 | Source Precision Medicine, Inc. | Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles |
US7553619B2 (en) * | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
EP2799555B1 (en) * | 2002-03-13 | 2017-02-22 | Genomic Health, Inc. | Gene expression profiling in biopsied tumor tissues |
EP2385139A1 (en) | 2002-07-31 | 2011-11-09 | University of Southern California | Polymorphisms for predicting disease and treatment outcome |
CN100374576C (zh) * | 2002-10-11 | 2008-03-12 | 阿克丘勒斯生物科学股份有限公司 | 来自ffpe样品的基因表达分布图 |
US7364846B2 (en) * | 2002-10-11 | 2008-04-29 | Molecular Devices Corporation | Gene expression profiling from FFPE samples |
EP1572105B1 (en) * | 2002-11-14 | 2011-08-31 | John Wayne Cancer Institute | Detection of micro metastasis of melanoma and breast cancer in paraffin-embedded tumor draining lymph nodes by multimaker quantitative rt-pcr |
US8039218B2 (en) * | 2002-11-14 | 2011-10-18 | John Wayne Cancer Institute | Detection of cancer cells in body fluids |
CA2506066A1 (en) | 2002-11-15 | 2004-06-03 | Genomic Health, Inc. | Gene expression profiling of egfr positive cancer |
US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
US20040231909A1 (en) | 2003-01-15 | 2004-11-25 | Tai-Yang Luh | Motorized vehicle having forward and backward differential structure |
EP1597391B1 (en) * | 2003-02-20 | 2008-10-29 | Genomic Health, Inc. | Use of intronic rna to measure gene expression |
WO2004080579A2 (en) | 2003-03-10 | 2004-09-23 | Expression Pathology, Inc. | Liquid tissue preparation from histopatologically processed biological samples, tissues and cells |
US8043834B2 (en) | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
US7267950B2 (en) * | 2003-05-01 | 2007-09-11 | Veridex, Lcc | Rapid extraction of RNA from cells and tissues |
EP1636380A2 (en) * | 2003-05-30 | 2006-03-22 | Genomic Health, Inc. | Gene expression markers for response to egfr inhibitor drugs |
US20040248103A1 (en) * | 2003-06-04 | 2004-12-09 | Feaver William John | Proximity-mediated rolling circle amplification |
JP4680898B2 (ja) | 2003-06-24 | 2011-05-11 | ジェノミック ヘルス, インコーポレイテッド | 癌再発の可能性の予測 |
ES2905579T3 (es) * | 2003-07-10 | 2022-04-11 | Genomic Health Inc | Algoritmo del perfil de expresión y prueba para el pronóstico de la recaída del cáncer de mama |
US8062897B2 (en) | 2003-07-21 | 2011-11-22 | Aureon Laboratories, Inc. | Diagnostic histopathology using multiplex gene expression FISH |
WO2005054466A2 (en) * | 2003-07-25 | 2005-06-16 | Ambion, Inc. | Methods and compositions for preparing rna from a fixed sample |
US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
US20050042660A1 (en) * | 2003-07-31 | 2005-02-24 | Hall Gerald Edward | Devices and methods for isolating RNA |
WO2005012487A2 (en) * | 2003-08-01 | 2005-02-10 | Invitrogen Corporation | Compositions and methods for preparing short rna molecules and other nucleic acids |
WO2005040396A2 (en) * | 2003-10-16 | 2005-05-06 | Genomic Health, Inc. | qRT-PCR ASSAY SYSTEM FOR GENE EXPRESSION PROFILING |
CA2543043C (en) * | 2003-10-24 | 2013-09-17 | The University Of Miami | Simplified tissue processing |
EP1689858B1 (en) | 2003-11-13 | 2013-05-15 | University Of Georgia Research Foundation, Inc. | Methods of characterizing infectious bursal disease virus |
WO2005064019A2 (en) * | 2003-12-23 | 2005-07-14 | Genomic Health, Inc. | Universal amplification of fragmented rna |
ES2550614T3 (es) | 2004-04-09 | 2015-11-11 | Genomic Health, Inc. | Marcadores de expresión génica para predecir la respuesta a la quimioterapia |
US7794932B2 (en) | 2004-04-16 | 2010-09-14 | Piotr Chomczynski | Reagents and methods for isolation of purified RNA |
US7588890B2 (en) * | 2004-04-16 | 2009-09-15 | Wei-Sing Chu | Device for extracting biological molecules from tissue specimens and methods for preparing the same |
US20050239055A1 (en) * | 2004-04-22 | 2005-10-27 | Mariani Brian D | Enterovirus primers and probes |
US7332597B2 (en) * | 2004-06-28 | 2008-02-19 | University Of Kentucky Research Foundation | Primers and probe to identify mycobacterium tuberculosis complex |
EP1774039A4 (en) | 2004-07-01 | 2009-07-08 | Univ Southern California | GENETIC MARKERS FOR PREDICTING DISEASES AND THERAPEUTIC RESULTS |
US20060099605A1 (en) * | 2004-11-11 | 2006-05-11 | Hall Gerald E Jr | Devices and methods for isolating RNA |
EP2458015B1 (en) | 2004-09-30 | 2015-12-23 | Epigenomics AG | Method for providing DNA fragments derived from an archived sample |
DE102004047772B4 (de) * | 2004-09-30 | 2018-12-13 | Infineon Technologies Ag | Lateraler Halbleitertransistor |
AU2005304824B2 (en) | 2004-11-05 | 2011-12-22 | Genomic Health, Inc. | Predicting response to chemotherapy using gene expression markers |
ATE550440T1 (de) * | 2004-11-05 | 2012-04-15 | Genomic Health Inc | Molekulare indikatoren für brustkrebsprognose und vorhersage des ansprechens auf eine behandlung |
US20060141481A1 (en) * | 2004-12-27 | 2006-06-29 | Mariani Brian D | HSV-1 and HSV-2 primers and probes |
US7541144B2 (en) * | 2005-01-31 | 2009-06-02 | Agilent Technologies, Inc. | RNA labeling method |
US8309303B2 (en) | 2005-04-01 | 2012-11-13 | Qiagen Gmbh | Reverse transcription and amplification of RNA with simultaneous degradation of DNA |
US7449297B2 (en) * | 2005-04-14 | 2008-11-11 | Euclid Diagnostics Llc | Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays |
EP2204454A1 (en) * | 2005-05-04 | 2010-07-07 | University of South Florida | Predicting treatment response in cancer subjects |
US20060270843A1 (en) * | 2005-05-26 | 2006-11-30 | Hall Gerald E Jr | Methods for isolation of nucleic acids |
US20060269929A1 (en) * | 2005-05-26 | 2006-11-30 | Hall Gerald E Jr | Methods and kits for DNA purification on polymeric membranes at low ionic strength |
US7544471B2 (en) | 2005-07-30 | 2009-06-09 | Agilent Technologies, Inc. | Preparing RNA from a wax-embedded tissue specimen |
US20070054168A1 (en) * | 2005-09-06 | 2007-03-08 | Hao Chang | Zinc/air cell |
EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
EP1777291A1 (en) * | 2005-10-20 | 2007-04-25 | Fundacion para la Investigacion Clinica y Molecular del Cancer de Pulmon | Method for the isolation of mRNA from formalin fixed, paraffin-embedded tissue |
US20070092883A1 (en) * | 2005-10-26 | 2007-04-26 | De Luwe Hoek Octrooien B.V. | Methylation specific multiplex ligation-dependent probe amplification (MS-MLPA) |
DE102005060738A1 (de) * | 2005-12-16 | 2007-06-21 | Qiagen Gmbh | Verfahren zur Extraktion von Biomolekülen aus fixierten Geweben |
CN100366632C (zh) * | 2005-12-28 | 2008-02-06 | 南方医科大学 | 一种石蜡包埋组织提取蛋白质的方法 |
CA2667521A1 (en) * | 2006-10-27 | 2008-05-15 | George Mason Intellectual Properties, Inc. | Assay for metastatic colorectal cancer |
ES2365447T3 (es) * | 2007-05-03 | 2011-10-05 | Roche Diagnostics Gmbh | Cuantificación del arnm de células individuales con rt-pcr en tiempo real. |
WO2008157316A1 (en) * | 2007-06-14 | 2008-12-24 | Creighton University | Improved method for rna isolation |
ES2401475T3 (es) * | 2007-06-15 | 2013-04-19 | University Of South Florida | Métodos de diagnóstico y tratamiento del cáncer |
JP2010530761A (ja) | 2007-06-22 | 2010-09-16 | レスポンス ジェネティクス,インコーポレイティド | 固定されたサンプルからの長い断片rnaの分離方法 |
AU2008323701B2 (en) | 2007-11-07 | 2015-03-26 | Genentech, Inc | Methods and compositions for assessing responsiveness of B-cell lymphoma to treatment with anti-CD40 antibodies |
EP2262893B1 (en) * | 2008-04-15 | 2013-04-24 | Universität Bern | Method for optimized isolation of rna from fixed tissue |
US20090298172A1 (en) * | 2008-05-28 | 2009-12-03 | Steven Paul Wheeler | Histological specimen treatment apparatus and method |
DE102008029356A1 (de) | 2008-06-20 | 2009-12-24 | Siemens Healthcare Diagnostics Gmbh | Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe |
ES2539856T3 (es) | 2008-08-01 | 2015-07-06 | F. Hoffmann-La Roche Ag | Lisis mejorada y transcripción reversa para la cuantificación de mRNA |
WO2010065775A2 (en) * | 2008-12-03 | 2010-06-10 | The United States Government As Represented By The Department Of Veterans Affairs | Pressure-assisted molecular recovery (pamr) of biomolecules, pressure-assisted antigen retrieval (paar), and pressure-assisted tissue histology (path) |
EP2193831A1 (de) | 2008-12-05 | 2010-06-09 | Qiagen GmbH | Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe |
DE102008061714A1 (de) | 2008-12-12 | 2010-06-17 | Siemens Healthcare Diagnostics Inc., Deerfield | Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe |
US8846878B1 (en) | 2009-01-23 | 2014-09-30 | Cubrc Corporation | Method and device for isolating a protein sample |
EP2210936A1 (en) * | 2009-01-27 | 2010-07-28 | Curetis AG | Processing and analysis of viscous liquid biological samples |
KR101787768B1 (ko) * | 2009-04-18 | 2017-10-18 | 제넨테크, 인크. | 항-cd40 항체를 사용한 치료에 대한 b-세포 림프종의 반응성 평가를 위한 방법 |
US20120100997A1 (en) | 2009-04-24 | 2012-04-26 | University Of Southern California | Cd133 polymorphisms and expression predict clinical outcome in patients with cancer |
DE102009031434B4 (de) * | 2009-07-01 | 2015-03-05 | Axagarius Gmbh & Co. Kg | Verwendung eines Kits für die Isolierung von Biomolekülen aus biologischen Proben sowie Kit hierfür |
WO2011038019A2 (en) | 2009-09-23 | 2011-03-31 | Danisco Us Inc. | Novel glycosyl hydrolase enzymes and uses thereof |
US8329360B2 (en) | 2009-12-04 | 2012-12-11 | Taiwan Semiconductor Manufacturing Company, Ltd. | Method and apparatus of providing overlay |
UA114276C2 (uk) | 2009-12-23 | 2017-05-25 | Даніско Юес Інк. | Спосіб одночасного оцукрювання і ферментації |
CN102803490B (zh) * | 2010-01-04 | 2015-03-25 | 奇亚根盖瑟斯堡股份有限公司 | 用于从固定组织样品回收核酸或蛋白质的方法、组合物和试剂盒 |
WO2011104027A1 (en) * | 2010-02-26 | 2011-09-01 | Qiagen Gmbh | Process for parallel isolation and/or purification of rna and dna |
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
EP2556158B1 (en) | 2010-04-08 | 2019-12-18 | Qiagen GmbH | Chromatographic device and method for isolating and purifying nucleic acids |
US9163228B2 (en) | 2010-04-08 | 2015-10-20 | Qiagen Gmbh | Method for isolating and purifying nucleic acids |
CN102869790B (zh) | 2010-04-12 | 2014-11-26 | 反应遗传有限公司 | Kras引物和探针 |
EP2388324A1 (de) * | 2010-05-20 | 2011-11-23 | Siemens Healthcare Diagnostics Inc. | Kontrollmaterial für Nukleinsäureteste |
EP2395082A1 (en) * | 2010-06-14 | 2011-12-14 | QIAGEN GmbH | Extraction of nucleic acids from wax-embedded samples |
CN103261873A (zh) * | 2010-10-12 | 2013-08-21 | 生命科技股份有限公司 | 制备用于ffpe的质量对照材料的方法 |
KR101420094B1 (ko) * | 2010-10-27 | 2014-07-17 | (주)바이오니아 | 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량pcr을 이용한 병원균의 전자동 생균수검사방법, 정량면역pcr을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법 |
TWI425977B (zh) | 2010-11-24 | 2014-02-11 | Ind Tech Res Inst | 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組 |
EP2468862A1 (en) * | 2010-12-23 | 2012-06-27 | Qiagen GmbH | Method and kit for processing wax-embedded biological samples |
CN102146112B (zh) * | 2011-01-25 | 2013-07-10 | 天根生化科技(北京)有限公司 | 从福尔马林固定石蜡包埋组织中提取脱氧核糖核酸的方法 |
CA2826056C (en) * | 2011-02-28 | 2021-01-26 | Dako Denmark A/S | Two phase immiscible system for the pretreatment of embedded biological samples |
CN102181431B (zh) * | 2011-03-11 | 2012-12-26 | 大连医科大学 | 从石蜡包埋组织中提取总rna的方法 |
BR112013023757A2 (pt) | 2011-03-17 | 2017-06-06 | Danisco Us Inc | método para redução de viscosidade no processo de sacarificação |
WO2012135125A1 (en) * | 2011-03-28 | 2012-10-04 | Geron Corporation | Telomere length measurement in formalin-fixed, paraffin embedded (ffpe) samples by quantitative pcr |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
EP2744916A4 (en) | 2011-07-13 | 2015-06-17 | Primeradx Inc | MULTIMODAL METHODS FOR SIMULTANEOUS DETECTION AND QUANTIFICATION OF MULTIPLE NUCLEIC ACIDS IN A SAMPLE |
FR2982278A1 (fr) | 2011-11-03 | 2013-05-10 | Assist Publ Hopitaux De Paris | Procede de classification d'echantillons de tissus fixes et inclus en paraffine |
US9416357B2 (en) | 2011-11-16 | 2016-08-16 | University Of Georgia Research Foundation, Inc. | Method for isolating total RNA from cells |
WO2013074885A1 (en) | 2011-11-17 | 2013-05-23 | Rheonix, Inc. | Microfluidic apparatus, method, and applications |
CN103571824B (zh) | 2012-08-09 | 2016-04-13 | 财团法人工业技术研究院 | 用于分离核酸的组合物及方法 |
EP2900819B1 (en) | 2012-09-28 | 2020-10-28 | Cepheid | Methods for dna and rna extraction from fixed paraffin-embedded tissue samples |
EP2906703A2 (en) | 2012-10-15 | 2015-08-19 | Pioneer Hi-Bred International, Inc. | Methods and compositions to enhance activity of cry endotoxins |
DK3511423T4 (da) | 2012-10-17 | 2024-07-29 | Spatial Transcriptomics Ab | Fremgangsmåder og produkt til optimering af lokaliseret eller rumlig detektion af genekspression i en vævsprøve |
US10968446B2 (en) | 2012-11-01 | 2021-04-06 | Massachusetts Institute Of Technology | Directed evolution of synthetic gene cluster |
EP2730653A1 (en) | 2012-11-07 | 2014-05-14 | QIAGEN GmbH | Method for lysing a fixed biological sample |
US9200327B2 (en) | 2012-11-30 | 2015-12-01 | Geron Corporation | Diagnostic markers for treating cell proliferative disorders with telomerase inhibitors |
EP2970363B1 (en) | 2013-03-14 | 2020-07-08 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
US10023877B2 (en) | 2013-03-15 | 2018-07-17 | Pioneer Hi-Bred International, Inc. | PHI-4 polypeptides and methods for their use |
KR101748867B1 (ko) * | 2013-04-19 | 2017-06-20 | 주식회사 젠큐릭스 | 조기 유방암 예후 예측 진단용 자동화 시스템 |
CA2916660C (en) | 2013-06-25 | 2022-05-17 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
EP3032942B1 (en) | 2013-08-16 | 2020-03-11 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
MX359027B (es) | 2013-09-13 | 2018-09-12 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
CN114763376A (zh) | 2014-02-07 | 2022-07-19 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
RU2747978C2 (ru) | 2014-02-07 | 2021-05-18 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
WO2016000237A1 (en) | 2014-07-03 | 2016-01-07 | Pioneer Overseas Corporation | Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes |
WO2016044092A1 (en) | 2014-09-17 | 2016-03-24 | Pioneer Hi Bred International Inc | Compositions and methods to control insect pests |
MX2017004745A (es) | 2014-10-16 | 2017-10-20 | Pioner Hi-Bred Int Inc | Proteinas insecticidas y metodos de uso. |
DE102014118532B4 (de) | 2014-12-12 | 2017-03-23 | Axagarius Gmbh & Co. Kg | Vorrichtung mit einer Filterschicht und Verfahren zur Gewinnung von Nukleinsäuren aus Formalin-fixierten und in Paraffin eingebetteten Proben |
US10968443B2 (en) * | 2014-12-31 | 2021-04-06 | The Rockefeller University | Method of RNA isolation from clinical samples |
WO2016144688A1 (en) | 2015-03-11 | 2016-09-15 | Pioneer Hi Bred International Inc | Insecticidal combinations of pip-72 and methods of use |
JP6828007B2 (ja) | 2015-04-10 | 2021-02-10 | スペーシャル トランスクリプトミクス アクチボラグ | 生物学的試料の空間識別されるマルチプレックスな核酸分析 |
US10365189B2 (en) | 2015-05-07 | 2019-07-30 | Steven Wheeler | Histological specimen treatment |
CN116333064A (zh) | 2015-05-19 | 2023-06-27 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
EA033170B1 (ru) * | 2015-05-21 | 2019-09-30 | Государственное Учреждение Образования "Белорусская Медицинская Академия Последипломного Образования" (Гуо "Белмапо") | Способ определения экспрессии гена gstm1 в образцах из парафин-фиксированных срезов опухолевой ткани молочной железы |
MX2017016119A (es) | 2015-06-16 | 2018-03-07 | Pioneer Hi Bred Int | Composiciones y metodos para controlar plagas de insectos. |
KR20230152796A (ko) | 2015-07-13 | 2023-11-03 | 피벗 바이오, 인크. | 식물 형질 개선을 위한 방법 및 조성물 |
EP3705582A1 (en) | 2015-07-24 | 2020-09-09 | Cepheid | Compositions and methods for dna and rna extraction from tissue samples |
WO2017023486A1 (en) | 2015-08-06 | 2017-02-09 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
ES2773072T3 (es) | 2015-08-28 | 2020-07-09 | Pioneer Hi Bred Int | Transformación de plantas mediada por Ochrobactrum |
US11479516B2 (en) | 2015-10-05 | 2022-10-25 | Massachusetts Institute Of Technology | Nitrogen fixation using refactored NIF clusters |
KR101749419B1 (ko) | 2015-10-15 | 2017-07-03 | 주식회사 어큐진 | 파라핀포매된 샘플로부터 dna를 추출하는 방법 |
CN108449995B (zh) | 2015-11-06 | 2022-02-01 | 文塔纳医疗系统公司 | 代表性诊断 |
EP3390431A1 (en) | 2015-12-18 | 2018-10-24 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
BR112018070754A2 (pt) | 2016-04-14 | 2019-02-12 | Pioneer Hi-Bred International, Inc. | polipeptídeos, polinucleotídeos, construção de dna, célula hospedeira, plantas ou célula vegetal, semente, composição, método para controlar uma população de pragas lepidópteras, método para exterminar uma praga de lepidóptero, método para produzir um polipeptídeo com atividade pesticida e método para proteger uma planta contra uma praga de inseto |
EP3445861B1 (en) | 2016-04-19 | 2021-12-08 | Pioneer Hi-Bred International, Inc. | Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof |
EP3451837B1 (en) | 2016-05-04 | 2021-08-25 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2017218207A1 (en) | 2016-06-16 | 2017-12-21 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
CN116334123A (zh) | 2016-06-24 | 2023-06-27 | 先锋国际良种公司 | 植物调节元件及其使用方法 |
MX2018015906A (es) | 2016-07-01 | 2019-04-04 | Pioneer Hi Bred Int | Proteinas insecticidas de plantas y metodos para sus usos. |
WO2018013333A1 (en) | 2016-07-12 | 2018-01-18 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
CN109863167B (zh) | 2016-11-01 | 2022-12-23 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
WO2018111551A1 (en) | 2016-12-14 | 2018-06-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CA3046226A1 (en) | 2016-12-22 | 2018-06-28 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3559271A1 (en) | 2016-12-22 | 2019-10-30 | Ventana Medical Systems, Inc. | Fully automated nucleic acid extraction method for tissue samples |
KR102698684B1 (ko) | 2017-01-12 | 2024-08-27 | 피벗 바이오, 인크. | 식물 형질 개선을 위한 방법 및 조성물 |
WO2018140214A1 (en) | 2017-01-24 | 2018-08-02 | Pioneer Hi-Bred International, Inc. | Nematicidal protein from pseudomonas |
MX2019009371A (es) | 2017-02-08 | 2019-09-23 | Pionner Hi Bred Int Inc | Combinaciones insecticidas de proteinas insecticidas derivadas de plantas y metodos para su uso. |
EA201992824A1 (ru) | 2017-05-26 | 2020-05-06 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные полипептиды, обладающие улучшенным спектром активности, и пути их применения |
CN107271239A (zh) * | 2017-07-06 | 2017-10-20 | 浙江大学 | 一种溶解石蜡组织切片的方法及应用 |
WO2019074598A1 (en) | 2017-10-13 | 2019-04-18 | Pioneer Hi-Bred International, Inc. | VIRUS-INDUCED GENETIC SILENCING TECHNOLOGY FOR THE CONTROL OF INSECTS IN MAIZE |
EP3701040A4 (en) | 2017-10-25 | 2021-08-25 | Pivot Bio, Inc. | METHODS AND COMPOSITIONS FOR IMPROVING GENETICALLY MODIFIED MICROBES THAT BIND NITROGEN |
KR20200123144A (ko) | 2018-02-22 | 2020-10-28 | 지머젠 인코포레이티드 | 바실러스가 농축된 게놈 라이브러리를 생성하고 새로운 cry 독소를 동정하기 위한 방법 |
WO2019169150A1 (en) | 2018-03-02 | 2019-09-06 | Pioneer Hi-Bred International, Inc. | Plant health assay |
BR112020017975A2 (pt) | 2018-03-02 | 2020-12-29 | Zymergen Inc. | Plataforma de descoberta de proteína inseticida e proteínas inseticidas descobertas a partir da mesma |
MX2020009435A (es) | 2018-03-14 | 2020-11-11 | Pioneer Hi Bred Int | Proteinas con actividad insecticida de plantas y metodos para sus usos. |
WO2019178038A1 (en) | 2018-03-14 | 2019-09-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
US11702668B2 (en) | 2018-05-22 | 2023-07-18 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
CA3103977A1 (en) | 2018-06-27 | 2020-01-02 | Pivot Bio, Inc. | Agricultural compositions comprising remodeled nitrogen fixing microbes |
MX2021002290A (es) | 2018-08-29 | 2021-04-28 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
US20220049294A1 (en) | 2018-12-10 | 2022-02-17 | 10X Genomics, Inc. | Imaging system hardware |
US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
WO2020243579A1 (en) | 2019-05-30 | 2020-12-03 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
CN110376036A (zh) * | 2019-07-30 | 2019-10-25 | 河南赛诺特生物技术有限公司 | 一种肿瘤细胞系免疫组化对照品的制备方法及肿瘤细胞系免疫组化对照品 |
CN110361242B (zh) * | 2019-08-14 | 2022-01-18 | 武汉赛维尔生物科技有限公司 | 一种用于眼球组织的固定液以及眼球组织制片的预处理方法 |
WO2021076346A1 (en) | 2019-10-18 | 2021-04-22 | Pioneer Hi-Bred International, Inc. | Maize event dp-202216-6 and dp-023211-2 stack |
WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
WO2021133845A1 (en) | 2019-12-23 | 2021-07-01 | 10X Genomics, Inc. | Reversible fixing reagents and methods of use thereof |
SG11202106899SA (en) | 2019-12-23 | 2021-09-29 | 10X Genomics Inc | Methods for spatial analysis using rna-templated ligation |
US20210190770A1 (en) | 2019-12-23 | 2021-06-24 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
TW202142114A (zh) | 2020-02-04 | 2021-11-16 | 美商陶氏農業科學公司 | 具有殺有害生物效用之組成物及與其相關之方法 |
US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
JP2023521579A (ja) | 2020-03-31 | 2023-05-25 | キアゲン ゲーエムベーハー | 固定された生体試料からの核酸精製 |
WO2021207610A1 (en) | 2020-04-10 | 2021-10-14 | 10X Genomics, Inc. | Cold protease treatment method for preparing biological samples |
CN115916972A (zh) | 2020-04-16 | 2023-04-04 | 10X基因组学有限公司 | 用于被固定样品的组合物和方法 |
EP4139485B1 (en) | 2020-04-22 | 2023-09-06 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
EP4153776A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
EP4414459A3 (en) | 2020-05-22 | 2024-09-18 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
ES2981265T3 (es) | 2020-06-08 | 2024-10-08 | 10X Genomics Inc | Métodos para determinar un margen quirúrgico y métodos de uso del mismo |
WO2021263111A1 (en) | 2020-06-25 | 2021-12-30 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
EP4182466A2 (en) | 2020-07-14 | 2023-05-24 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CN116323968A (zh) | 2020-07-31 | 2023-06-23 | 10X基因组学有限公司 | 用于空间分析的去交联化合物和使用方法 |
WO2022035649A1 (en) | 2020-08-10 | 2022-02-17 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
EP4244379A1 (en) | 2020-11-13 | 2023-09-20 | 10X Genomics, Inc. | Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays |
US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
CN114854824A (zh) * | 2021-02-03 | 2022-08-05 | 深圳华大基因股份有限公司 | 建库方法及应用 |
EP4347879A1 (en) | 2021-06-03 | 2024-04-10 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
US20230071935A1 (en) * | 2021-08-25 | 2023-03-09 | Matchstick Technologies, Inc. | Microplate cycling thermo sonicator with programmable ultrasound, heating, and cooling for multi-analytical applications |
WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
TW202345696A (zh) | 2022-05-18 | 2023-12-01 | 美商科迪華農業科技有限責任公司 | 具有殺有害生物效用之組成物及與其相關的方法 |
WO2023245190A1 (en) | 2022-06-17 | 2023-12-21 | 10X Genomics, Inc. | Catalytic de-crosslinking of samples for in situ analysis |
Family Cites Families (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4830969A (en) | 1981-08-31 | 1989-05-16 | The Research Foundation Of State University Of New York | Process for the rapid and simple isolation of nucleic acids |
US4843155A (en) | 1987-11-19 | 1989-06-27 | Piotr Chomczynski | Product and process for isolating RNA |
US5128247A (en) | 1989-08-14 | 1992-07-07 | Board Of Regents, The University Of Texas System | Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources |
US5284940A (en) | 1990-11-14 | 1994-02-08 | Hri Research, Inc. | Preparation for nucleic acid samples |
US5620852A (en) | 1990-11-14 | 1997-04-15 | Hri Research, Inc. | Nucleic acid preparation methods |
US5654179A (en) * | 1990-11-14 | 1997-08-05 | Hri Research, Inc. | Nucleic acid preparation methods |
US5346994A (en) * | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
US5502166A (en) | 1992-02-26 | 1996-03-26 | Mitsubishi Chemical Corporation | NMDH receptor proteins and genes encoding the same |
AU6230594A (en) | 1993-02-01 | 1994-08-29 | University Of Iowa Research Foundation, The | Quartenary amine surfactants and methods of using same in isolation of rna |
US5876978A (en) * | 1993-04-06 | 1999-03-02 | Medical College Of Ohio | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
US5637687A (en) | 1993-08-31 | 1997-06-10 | Wiggins; James C. | Methods and compositions for isolating nucleic acids |
US5643767A (en) | 1994-05-02 | 1997-07-01 | The Rockefeller University | Process for isolating cellular components |
US5942391A (en) * | 1994-06-22 | 1999-08-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method: ramification-extension amplification method (RAM) |
US5876924A (en) * | 1994-06-22 | 1999-03-02 | Mount Sinai School Of Medicine | Nucleic acid amplification method hybridization signal amplification method (HSAM) |
CA2153215A1 (en) | 1994-07-06 | 1996-01-07 | Lu Wang | Treatment of paraffin embedded tissue for gene analysis |
US5763169A (en) * | 1995-01-13 | 1998-06-09 | Chiron Diagnostics Corporation | Nucleic acid probes for the detection and identification of fungi |
US5707802A (en) * | 1995-01-13 | 1998-01-13 | Ciba Corning Diagnostics Corp. | Nucleic acid probes for the detection and identification of fungi |
US5777099A (en) | 1995-02-24 | 1998-07-07 | Biotecx Laboratories, Inc. | RNA separation |
US5945515A (en) | 1995-07-31 | 1999-08-31 | Chomczynski; Piotr | Product and process for isolating DNA, RNA and proteins |
US5998151A (en) | 1995-12-01 | 1999-12-07 | The United States Of America As Represented By The Department Of Health And Human Services | Methods for predicting the efficacy of a chemotherapeutic regimen for gastrointestinal cancers using antibodies specific for thymidylate synthase |
US20020090642A1 (en) * | 1995-12-11 | 2002-07-11 | Patrick G. Johnston | Methods for determining the prognosis of breast cancer using antibodies specific for thymidylate synthase |
DE29601618U1 (de) | 1996-01-31 | 1996-07-04 | InViTek GmbH, 13125 Berlin | Vorrichtung zur gleichzeitigen multiplen Isolierung |
EP0791654A1 (en) | 1996-02-21 | 1997-08-27 | Jürgen A. Dr. Richt | Polypeptides corresponding to the amino acid sequences of proteins p57 or p9.5 of Borna disease virus, nucleic acid fragments coding therefore and their use for diagnostic and immunization purposes |
EP0912726A4 (en) * | 1996-05-10 | 2001-07-18 | Phylomed Corp | METHODS FOR OXIDIZING BISULFIDE LINKS WITH OZONE |
CA2284686A1 (en) * | 1997-03-31 | 1998-10-08 | Abbott Laboratories | Reagents and methods useful for detecting diseases of the gastrointestinal tract |
CA2366231C (en) * | 1999-03-18 | 2010-05-11 | Exiqon A/S | One step sample preparation and detection of nucleic acids in complex biological samples |
US6248535B1 (en) * | 1999-12-20 | 2001-06-19 | University Of Southern California | Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens |
-
1999
- 1999-12-20 US US09/469,338 patent/US6248535B1/en not_active Expired - Lifetime
-
2000
- 2000-10-31 EA EA200501772A patent/EA008832B1/ru not_active IP Right Cessation
- 2000-10-31 MX MXPA02006145A patent/MXPA02006145A/es active IP Right Grant
- 2000-10-31 AU AU15785/01A patent/AU784565C/en not_active Ceased
- 2000-10-31 DK DK06123097.5T patent/DK1743939T3/da active
- 2000-10-31 CN CNB008174741A patent/CN1269959C/zh not_active Expired - Fee Related
- 2000-10-31 AT AT00978311T patent/ATE349518T1/de active
- 2000-10-31 ES ES06123097T patent/ES2373149T3/es not_active Expired - Lifetime
- 2000-10-31 CA CA2394618A patent/CA2394618C/en not_active Expired - Fee Related
- 2000-10-31 IL IL15011600A patent/IL150116A0/xx unknown
- 2000-10-31 EP EP06123097A patent/EP1743939B1/en not_active Expired - Lifetime
- 2000-10-31 EP EP00978311A patent/EP1242594B1/en not_active Expired - Lifetime
- 2000-10-31 DE DE60032634T patent/DE60032634T2/de not_active Expired - Lifetime
- 2000-10-31 EA EA200200698A patent/EA006827B1/ru not_active IP Right Cessation
- 2000-10-31 CN CN200610093878XA patent/CN1891836B/zh not_active Expired - Fee Related
- 2000-10-31 JP JP2001546900A patent/JP2003525033A/ja active Pending
- 2000-10-31 WO PCT/US2000/030012 patent/WO2001046402A1/en active IP Right Grant
- 2000-10-31 KR KR1020027007849A patent/KR100723328B1/ko not_active IP Right Cessation
- 2000-10-31 AT AT06123097T patent/ATE523590T1/de active
- 2000-10-31 BR BR0016569-7A patent/BR0016569A/pt not_active Application Discontinuation
- 2000-10-31 EP EP10182481.1A patent/EP2363477A3/en not_active Withdrawn
- 2000-12-20 TW TW089127378A patent/TWI277652B/zh not_active IP Right Cessation
- 2000-12-20 AR ARP000106793A patent/AR027036A1/es active IP Right Grant
-
2001
- 2001-03-01 US US09/797,158 patent/US6428963B2/en not_active Expired - Lifetime
- 2001-03-01 US US09/797,163 patent/US6610488B2/en not_active Expired - Lifetime
- 2001-03-01 US US09/797,216 patent/US6613518B2/en not_active Expired - Lifetime
-
2002
- 2002-06-10 IL IL150116A patent/IL150116A/en not_active IP Right Cessation
- 2002-06-19 NO NO20022945A patent/NO330802B1/no not_active IP Right Cessation
-
2003
- 2003-07-08 US US10/616,203 patent/US20040091910A1/en not_active Abandoned
- 2003-07-30 US US10/631,413 patent/US7960147B2/en not_active Expired - Fee Related
- 2003-10-28 HK HK03107749A patent/HK1055446A1/xx not_active IP Right Cessation
-
2006
- 2006-04-05 US US11/278,804 patent/US7998707B2/en not_active Expired - Fee Related
- 2006-07-12 AU AU2006202986A patent/AU2006202986B2/en not_active Ceased
-
2007
- 2007-04-26 HK HK07104456.2A patent/HK1098178A1/xx not_active IP Right Cessation
- 2007-07-27 US US11/881,625 patent/US7919280B2/en not_active Expired - Fee Related
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
NO330802B1 (no) | Metode for gjenvinning av RNA fra formalin-fiksert paraffin-innstopt vevsprove, metode for kvantitativ maling av genekspresjon av et malgen og metode for maling av niva av malgenekspresjon i en fiksert paraffin-innstopt vevsprove. | |
US11965156B2 (en) | RNA isolation from soluble urine fractions | |
US20140308670A1 (en) | Simultaneous extraction of dna and rna from ffpe tissues | |
CA2626533C (en) | Method for the isolation of mrna from formalin fixed, paraffin-embedded tissue | |
CN111278844B (zh) | 用于使用氧化铁颗粒提取核酸的方法和组合物 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM1K | Lapsed by not paying the annual fees |