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MXPA02006145A - Metodo para el aislamiento de arn de especimenes de tejido incluido en parafina fijo en formalina. - Google Patents

Metodo para el aislamiento de arn de especimenes de tejido incluido en parafina fijo en formalina.

Info

Publication number
MXPA02006145A
MXPA02006145A MXPA02006145A MXPA02006145A MXPA02006145A MX PA02006145 A MXPA02006145 A MX PA02006145A MX PA02006145 A MXPA02006145 A MX PA02006145A MX PA02006145 A MXPA02006145 A MX PA02006145A MX PA02006145 A MXPA02006145 A MX PA02006145A
Authority
MX
Mexico
Prior art keywords
rna
formalinfixed
isolation
tissue specimens
paraffinembedded
Prior art date
Application number
MXPA02006145A
Other languages
English (en)
Inventor
Steven Swenson
Original Assignee
Univ Southern California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Southern California filed Critical Univ Southern California
Publication of MXPA02006145A publication Critical patent/MXPA02006145A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se describen metodos para el aislamiento rapido: confiable y simple de ARN de muestras de tejido incluido en parafina fijo en formalina. El ARN purificado de esta manera puede utilizarse para monitorear los niveles de expresion genica. La muestra de tejido puede ser un tumor u otro tejido patologico.
MXPA02006145A 1999-12-20 2000-10-31 Metodo para el aislamiento de arn de especimenes de tejido incluido en parafina fijo en formalina. MXPA02006145A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/469,338 US6248535B1 (en) 1999-12-20 1999-12-20 Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
PCT/US2000/030012 WO2001046402A1 (en) 1999-12-20 2000-10-31 Method for isolation of rna from formalin-fixed paraffin-embedded tissue specimens

Publications (1)

Publication Number Publication Date
MXPA02006145A true MXPA02006145A (es) 2004-08-23

Family

ID=23863395

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02006145A MXPA02006145A (es) 1999-12-20 2000-10-31 Metodo para el aislamiento de arn de especimenes de tejido incluido en parafina fijo en formalina.

Country Status (20)

Country Link
US (8) US6248535B1 (es)
EP (3) EP1743939B1 (es)
JP (1) JP2003525033A (es)
KR (1) KR100723328B1 (es)
CN (2) CN1269959C (es)
AR (1) AR027036A1 (es)
AT (2) ATE349518T1 (es)
AU (2) AU784565C (es)
BR (1) BR0016569A (es)
CA (1) CA2394618C (es)
DE (1) DE60032634T2 (es)
DK (1) DK1743939T3 (es)
EA (2) EA008832B1 (es)
ES (1) ES2373149T3 (es)
HK (2) HK1055446A1 (es)
IL (2) IL150116A0 (es)
MX (1) MXPA02006145A (es)
NO (1) NO330802B1 (es)
TW (1) TWI277652B (es)
WO (1) WO2001046402A1 (es)

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