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ATE349518T1 - Verfahren zur isolierung von rns aus fixierten paraffin-eingebetteten gewebeproben - Google Patents

Verfahren zur isolierung von rns aus fixierten paraffin-eingebetteten gewebeproben

Info

Publication number
ATE349518T1
ATE349518T1 AT00978311T AT00978311T ATE349518T1 AT E349518 T1 ATE349518 T1 AT E349518T1 AT 00978311 T AT00978311 T AT 00978311T AT 00978311 T AT00978311 T AT 00978311T AT E349518 T1 ATE349518 T1 AT E349518T1
Authority
AT
Austria
Prior art keywords
rna
isolation
tissue samples
embedded tissue
fixed paraffin
Prior art date
Application number
AT00978311T
Other languages
English (en)
Inventor
Kathleen Danenberg
Peter Danenberg
Steven Swenson
Original Assignee
Univ Southern California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Southern California filed Critical Univ Southern California
Application granted granted Critical
Publication of ATE349518T1 publication Critical patent/ATE349518T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
AT00978311T 1999-12-20 2000-10-31 Verfahren zur isolierung von rns aus fixierten paraffin-eingebetteten gewebeproben ATE349518T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/469,338 US6248535B1 (en) 1999-12-20 1999-12-20 Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens

Publications (1)

Publication Number Publication Date
ATE349518T1 true ATE349518T1 (de) 2007-01-15

Family

ID=23863395

Family Applications (2)

Application Number Title Priority Date Filing Date
AT00978311T ATE349518T1 (de) 1999-12-20 2000-10-31 Verfahren zur isolierung von rns aus fixierten paraffin-eingebetteten gewebeproben
AT06123097T ATE523590T1 (de) 1999-12-20 2000-10-31 Verfahren zur isolierung von rna aus formalin- fixierten paraffin-eingebetteten gewebeproben

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT06123097T ATE523590T1 (de) 1999-12-20 2000-10-31 Verfahren zur isolierung von rna aus formalin- fixierten paraffin-eingebetteten gewebeproben

Country Status (20)

Country Link
US (8) US6248535B1 (de)
EP (3) EP1743939B1 (de)
JP (1) JP2003525033A (de)
KR (1) KR100723328B1 (de)
CN (2) CN1269959C (de)
AR (1) AR027036A1 (de)
AT (2) ATE349518T1 (de)
AU (2) AU784565C (de)
BR (1) BR0016569A (de)
CA (1) CA2394618C (de)
DE (1) DE60032634T2 (de)
DK (1) DK1743939T3 (de)
EA (2) EA008832B1 (de)
ES (1) ES2373149T3 (de)
HK (2) HK1055446A1 (de)
IL (2) IL150116A0 (de)
MX (1) MXPA02006145A (de)
NO (1) NO330802B1 (de)
TW (1) TWI277652B (de)
WO (1) WO2001046402A1 (de)

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