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SI1987150T1 - Selekcija gostiteljskih celic, ki eksprimirajo protein pri visokih nivojih - Google Patents

Selekcija gostiteljskih celic, ki eksprimirajo protein pri visokih nivojih

Info

Publication number
SI1987150T1
SI1987150T1 SI200730698T SI200730698T SI1987150T1 SI 1987150 T1 SI1987150 T1 SI 1987150T1 SI 200730698 T SI200730698 T SI 200730698T SI 200730698 T SI200730698 T SI 200730698T SI 1987150 T1 SI1987150 T1 SI 1987150T1
Authority
SI
Slovenia
Prior art keywords
polypeptide
interest
selectable marker
host cells
coding
Prior art date
Application number
SI200730698T
Other languages
English (en)
Inventor
Arie Pieter Otte
Blokland Henricus Johannes Maria Van
Theodorus Hendrikus Jacobus Kwaks
Richard George Antonius Bernardus Sewalt
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/359,953 external-priority patent/US20060141577A1/en
Priority claimed from US11/416,490 external-priority patent/US20060195935A1/en
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Publication of SI1987150T1 publication Critical patent/SI1987150T1/sl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/40—Vector systems having a special element relevant for transcription being an insulator
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SI200730698T 2006-02-21 2007-02-21 Selekcija gostiteljskih celic, ki eksprimirajo protein pri visokih nivojih SI1987150T1 (sl)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11/359,953 US20060141577A1 (en) 2004-11-08 2006-02-21 Selection of host cells expressing protein at high levels
EP06113354 2006-05-02
US11/416,490 US20060195935A1 (en) 2004-11-08 2006-05-02 Selection of host cells expressing protein at high levels
PCT/EP2007/051696 WO2007096399A2 (en) 2006-02-21 2007-02-21 Selection of host cells expressing protein at high levels
EP07712283A EP1987150B1 (en) 2006-02-21 2007-02-21 Selection of host cells expressing protein at high levels

Publications (1)

Publication Number Publication Date
SI1987150T1 true SI1987150T1 (sl) 2011-09-30

Family

ID=38324190

Family Applications (1)

Application Number Title Priority Date Filing Date
SI200730698T SI1987150T1 (sl) 2006-02-21 2007-02-21 Selekcija gostiteljskih celic, ki eksprimirajo protein pri visokih nivojih

Country Status (14)

Country Link
EP (2) EP1987150B1 (sl)
JP (1) JP5225107B2 (sl)
KR (1) KR101328300B1 (sl)
CN (2) CN101389763B (sl)
AT (2) ATE511544T1 (sl)
AU (1) AU2007247242B2 (sl)
CA (2) CA2637271C (sl)
DK (2) DK1987150T3 (sl)
EA (1) EA014332B1 (sl)
HK (1) HK1128490A1 (sl)
PL (1) PL1987150T3 (sl)
PT (1) PT1987150E (sl)
SI (1) SI1987150T1 (sl)
WO (2) WO2007096399A2 (sl)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1572994T3 (da) 2002-12-20 2007-05-29 Chromagenics Bv Midler og fremgangsmåder til fremstilling af en protein gennem chromatinåbnere, som kan göre chromatin mere tilgængeligt for transkriptionsfaktorer
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US7968700B2 (en) 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof
JP5704753B2 (ja) 2008-12-22 2015-04-22 国立大学法人北海道大学 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用
ES2587630T3 (es) 2009-02-27 2016-10-25 Novartis Ag Sistema de vector de expresión que comprende dos marcadores de selección
US8765370B2 (en) * 2009-06-11 2014-07-01 Scinopharm Taiwan, Ltd Inhibition-based high-throughput screen strategy for cell clones
BRPI1013141A2 (pt) 2009-06-15 2015-10-06 Cellagenics B V novos marcadores selecionaveis rigorosos
WO2011113841A1 (en) * 2010-03-16 2011-09-22 Robert Steinfeld Eukaryotic vector
CN103037840A (zh) * 2010-04-21 2013-04-10 国立大学法人北海道大学 具有核内转运性的脂质膜结构体
WO2011159157A1 (en) 2010-06-15 2011-12-22 Cellagenics B.V. Novel intergenic elements for enhancing gene expression
WO2012030218A1 (en) 2010-09-01 2012-03-08 Cellagenics B.V. Nucleic acid fragments from a ribosomal protein promoter for enhancing gene expression
DK3027646T3 (en) 2013-07-31 2018-09-24 Novartis Ag New selection vectors and methods for selecting eukaryotic host cells
CN104531699B (zh) * 2014-10-09 2017-03-15 河南农业大学 一种增强外源基因表达的猪的ucoe调控元件片段
CN106754817B (zh) * 2016-11-03 2021-02-05 山西省生物研究院有限公司 一种重组自分泌运动因子的表达方法
DE102017103383A1 (de) * 2017-02-20 2018-08-23 aReNA-Bio GbR (vertretungsberechtigter Gesellschafter: Dr. Heribert Bohlen, 50733 Köln) System und Verfahren zur Zelltyp-spezifischen Translation von RNA-Molekülen in Eukaryoten
TWI802728B (zh) * 2018-07-30 2023-05-21 大陸商南京金斯瑞生物科技有限公司 密碼子優化方法、包括其之系統及電子裝置、其核酸分子及使用其之蛋白質表現方法
WO2020036181A1 (ja) * 2018-08-13 2020-02-20 Ｓｐｉｂｅｒ株式会社 細胞を単離又は同定する方法及び細胞集団
CN110484563B (zh) * 2019-07-25 2023-04-07 新乡医学院 哺乳动物细胞组合表达载体、表达系统、制备方法和应用
KR20210080071A (ko) * 2019-12-20 2021-06-30 (주)셀트리온 목적 단백질의 고발현을 위한 인트론을 포함하는 발현 카세트 및 이의 이용

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5256568A (en) * 1990-02-12 1993-10-26 Regeneron Phamaceuticals, Inc. Vectors and transformed most cells for recombinant protein production with reduced expression of selectable markers
US6319707B1 (en) * 1991-08-12 2001-11-20 Fred Hutchinson Cancer Research Center Board Regents Of The University Of Washington Cap-independent multicistronic retroviral vectors
US5648267A (en) * 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
WO1996012008A1 (en) * 1994-10-13 1996-04-25 Merck & Co., Inc. Synthesis of methylase-resistant genes
DK1513937T3 (da) * 2002-06-14 2011-05-16 Chromagenics Bv Fremgangsmåde til den samtidige produktion af multiple proteiner; vektorer og celler til anvendelse derved
ATE550432T1 (de) * 2004-11-08 2012-04-15 Chromagenics Bv Selektion von wirtszellen mit proteinexpression auf hohem niveau
WO2006048459A2 (en) * 2004-11-08 2006-05-11 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels

Also Published As

Publication number Publication date
PL1987150T3 (pl) 2011-10-31
EP2013349B1 (en) 2010-11-17
CA2651088C (en) 2016-03-08
CN101437946B (zh) 2012-07-04
WO2007128685A1 (en) 2007-11-15
CN101437946A (zh) 2009-05-20
EP1987150A2 (en) 2008-11-05
CA2637271A1 (en) 2007-08-30
PT1987150E (pt) 2011-09-08
WO2007096399A3 (en) 2008-05-02
KR20080105034A (ko) 2008-12-03
AU2007247242B2 (en) 2012-03-08
DK2013349T3 (da) 2011-02-28
AU2007247242A1 (en) 2007-11-15
CA2651088A1 (en) 2007-11-15
CA2637271C (en) 2014-12-09
KR101328300B1 (ko) 2013-11-14
HK1128490A1 (en) 2009-10-30
DK1987150T3 (da) 2011-09-19
JP5225107B2 (ja) 2013-07-03
CN101389763B (zh) 2013-01-23
ATE488593T1 (de) 2010-12-15
JP2009527240A (ja) 2009-07-30
ATE511544T1 (de) 2011-06-15
CN101389763A (zh) 2009-03-18
AU2007217431A1 (en) 2007-08-30
WO2007096399A2 (en) 2007-08-30
EA200870279A1 (ru) 2009-02-27
EP2013349A1 (en) 2009-01-14
EP1987150B1 (en) 2011-06-01
EA014332B1 (ru) 2010-10-29

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