MX338911B - Identificacion mediada por trasposición de proteínas de unión o funcionales específicas. - Google Patents
Identificacion mediada por trasposición de proteínas de unión o funcionales específicas.Info
- Publication number
- MX338911B MX338911B MX2015000885A MX2015000885A MX338911B MX 338911 B MX338911 B MX 338911B MX 2015000885 A MX2015000885 A MX 2015000885A MX 2015000885 A MX2015000885 A MX 2015000885A MX 338911 B MX338911 B MX 338911B
- Authority
- MX
- Mexico
- Prior art keywords
- binding
- functional
- fragments
- host cells
- expressed proteins
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención se refiere a un método de tecnología novedosa que ofrece eficiencia no paralela, flexibilidad, utilidad y velocidad para el descubrimiento y optimización de polipéptidos que tienen especificidad y/o funcionalidad de unión deseada, que incluye moléculas de unión al antígeno tales como anticuerpos y fragmentos de los mismos, para los fenotipos funcionales y/o de unión deseados. El método novedoso se base en constructos de transposición y diversas bibliotecas de ADN clonadas en vectores de transposición y su transfección en células huésped por expresión temporal concomitante de una enzima de transposasa funcional. Esto asegura una introducción eficiente y estable de los vectores de expresión basados en transposón en células huésped de vertebrado en una etapa, que pueden clasificarse entonces por un fenotipo funcional o de unión deseado de las proteínas expresadas, después de lo cual las secuencias de codificación relevantes para las proteínas expresadas, que incluyen anticuerpos y fragmentos de los mismos, pueden identificarse por técnicas de clonación y secuenciación de ADN estándar.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261673144P | 2012-07-18 | 2012-07-18 | |
| EP12178529.9A EP2692865B1 (en) | 2012-07-30 | 2012-07-30 | Transposition-mediated identification of specific binding or functional proteins |
| PCT/EP2013/065214 WO2014013026A1 (en) | 2012-07-18 | 2013-07-18 | Transposition-mediated identification of specific binding or functional proteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2015000885A MX2015000885A (es) | 2015-07-14 |
| MX338911B true MX338911B (es) | 2016-05-05 |
Family
ID=49775745
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015000885A MX338911B (es) | 2012-07-18 | 2013-07-18 | Identificacion mediada por trasposición de proteínas de unión o funcionales específicas. |
Country Status (19)
| Country | Link |
|---|---|
| US (2) | US10174309B2 (es) |
| EP (2) | EP2692865B1 (es) |
| JP (1) | JP6043431B2 (es) |
| CN (1) | CN104640985B (es) |
| AU (1) | AU2013291971B2 (es) |
| BR (1) | BR112015000836B1 (es) |
| CA (1) | CA2878982C (es) |
| DK (1) | DK2692865T3 (es) |
| ES (1) | ES2528892T3 (es) |
| HK (1) | HK1209779A1 (es) |
| IL (1) | IL236771A (es) |
| IN (1) | IN2015DN01301A (es) |
| MX (1) | MX338911B (es) |
| NZ (1) | NZ705074A (es) |
| PT (1) | PT2692865E (es) |
| SG (1) | SG11201500280XA (es) |
| SI (1) | SI2692865T1 (es) |
| WO (1) | WO2014013026A1 (es) |
| ZA (1) | ZA201500203B (es) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| EP3368574A1 (en) | 2015-10-30 | 2018-09-05 | NBE-Therapeutics AG | Anti-ror1 antibodies |
| CA3011815A1 (en) | 2016-01-20 | 2017-07-27 | The Scripps Research Institute | Ror1 antibody compositions and related methods |
| SG11201806121PA (en) | 2016-01-20 | 2018-08-30 | Scripps Research Inst | Ror2 antibody compositions and related methods |
| KR20180132705A (ko) * | 2016-04-04 | 2018-12-12 | 에테하 취리히 | 단백질 생산 및 라이브러리(Library) 생성을 위한 포유동물 세포주 |
| AU2017371179B2 (en) * | 2016-12-09 | 2022-07-14 | Gliknik Inc. | Manufacturing optimization of GL-2045, a multimerizing stradomer |
| EP3551227A4 (en) | 2016-12-09 | 2020-07-29 | Gliknik Inc. | METHOD FOR TREATING INFLAMMABLE DISEASES WITH MULTI-VALUE FC COMPOUNDS |
| JP7275043B2 (ja) | 2016-12-16 | 2023-05-17 | ビー－モーゲン・バイオテクノロジーズ，インコーポレーテッド | 増大したｈＡＴファミリートランスポゾン媒介遺伝子導入ならびに関連する組成物、システムおよび方法 |
| US11278570B2 (en) | 2016-12-16 | 2022-03-22 | B-Mogen Biotechnologies, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
| US11447546B2 (en) | 2017-07-20 | 2022-09-20 | Nbe-Therapeutics Ag | Human antibodies binding to ROR2 |
| KR20230008269A (ko) | 2017-08-07 | 2023-01-13 | 엔비이-테라퓨틱스 아게 | 생체 내 내성이 높은 항체 약물 결합체 |
| US20210214747A1 (en) * | 2018-05-18 | 2021-07-15 | Sorbonne Universite | Molecular tools and methods for transgene integration and their transposition dependent expression |
| WO2019246486A2 (en) | 2018-06-21 | 2019-12-26 | B-Mogen Biotechnologies, Inc. | ENHANCED hAT FAMILY TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS |
| SG11202103334YA (en) * | 2018-10-26 | 2021-05-28 | Hoffmann La Roche | Multispecific antibody screening method using recombinase mediated cassette exchange |
| JP2020146014A (ja) * | 2019-03-15 | 2020-09-17 | 株式会社東芝 | トランスポゼース活性測定用ベクター、トランスポゼース活性測定用キット、トランスポゼース活性測定方法及び細胞分離方法 |
| BR112022003970A2 (pt) | 2019-09-03 | 2022-06-21 | Myeloid Therapeutics Inc | Métodos e composições para integração genômica |
| CN111139264B (zh) * | 2020-01-20 | 2021-07-06 | 天津达济科技有限公司 | 一种基于线性双链dna分子在哺乳动物细胞系内构建单域抗体库的方法 |
| CN111471714A (zh) * | 2020-05-07 | 2020-07-31 | 西南大学 | Minos转座子系统介导的真核生物转基因细胞系及构建方法 |
| EP4271817A2 (en) | 2020-12-30 | 2023-11-08 | Alaunos Therapeutics, Inc. | Recombinant vectors comprising polycistronic expression cassettes and methods of use thereof |
| JP2024507929A (ja) | 2021-02-25 | 2024-02-21 | アラウノス セラピューティクス インコーポレイテッド | 多シストロン性発現カセットを含む組換えベクター及びそれらの使用方法 |
| MX2023011638A (es) * | 2021-03-31 | 2023-10-13 | Ddbio Co Ltd Shang Hai | Vectores y metodo para el cribado de proteinas de union a antigeno funcionales. |
| WO2023150562A1 (en) | 2022-02-01 | 2023-08-10 | Alaunos Therapeutics, Inc. | Methods for activation and expansion of t cells |
| WO2024102954A1 (en) | 2022-11-10 | 2024-05-16 | Massachusetts Institute Of Technology | Activation induced clipping system (aics) |
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| US5223409A (en) * | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| US5667988A (en) * | 1992-01-27 | 1997-09-16 | The Scripps Research Institute | Methods for producing antibody libraries using universal or randomized immunoglobulin light chains |
| US6090382A (en) | 1996-02-09 | 2000-07-18 | Basf Aktiengesellschaft | Human antibodies that bind human TNFα |
| US6218185B1 (en) | 1996-04-19 | 2001-04-17 | The United States Of America As Represented By The Secretary Of Agriculture | Piggybac transposon-based genetic transformation system for insects |
| WO1998040510A1 (en) | 1997-03-11 | 1998-09-17 | Regents Of The University Of Minnesota | Dna-based transposon system for the introduction of nucleic acid into dna of a cell |
| US7160682B2 (en) | 1998-11-13 | 2007-01-09 | Regents Of The University Of Minnesota | Nucleic acid transfer vector for the introduction of nucleic acid into the DNA of a cell |
| US7105343B1 (en) | 2000-10-31 | 2006-09-12 | University Of Notre Dame Du Lac | Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector Piggybac |
| US6962810B2 (en) | 2000-10-31 | 2005-11-08 | University Of Notre Dame Du Lac | Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector piggyBac |
| HUP0400118A2 (hu) | 2001-06-14 | 2005-02-28 | Basf Corporation | Fénystabilizáló gyököket tartalmazó fénystabilizált polimerek, ezekből készült cikkek és eljárás előállításukra |
| WO2003089618A2 (en) | 2002-04-22 | 2003-10-30 | Regents Of The University Of Minnesota | Transposon system and methods of use |
| DE60319259T2 (de) * | 2002-06-14 | 2009-03-05 | Dyax Corp., Cambridge | Rekombination von nukleinsäurebibliotheksmitgliedern |
| US20050182250A1 (en) | 2002-07-10 | 2005-08-18 | Stratagene | Humanized renilla reniformis green fluorescent protein as a scaffold |
| EP1545613B9 (en) | 2002-07-31 | 2012-01-25 | Seattle Genetics, Inc. | Auristatin conjugates and their use for treating cancer, an autoimmune disease or an infectious disease |
| US8071364B2 (en) * | 2003-12-24 | 2011-12-06 | Transgenrx, Inc. | Gene therapy using transposon-based vectors |
| US7932088B1 (en) | 2005-04-25 | 2011-04-26 | University Of Notre Dame Du Lac | High efficiency transformation of Plasmodium falciparum by the lepidopteran transposon, piggyBac |
| US20060252140A1 (en) | 2005-04-29 | 2006-11-09 | Yant Stephen R | Development of a transposon system for site-specific DNA integration in mammalian cells |
| WO2007046439A1 (ja) * | 2005-10-18 | 2007-04-26 | National Institute Of Agrobiological Sciences | 抗体を産生するトランスジェニックカイコとその製造方法 |
| US20090042297A1 (en) * | 2007-06-01 | 2009-02-12 | George Jr Alfred L | Piggybac transposon-based vectors and methods of nucleic acid integration |
| EP2160461B1 (en) | 2007-07-04 | 2012-08-08 | Max-Delbrück-Centrum für Molekulare Medizin (MDC) | Hyperactive variants of the transposase protein of the transposon system sleeping beauty |
| CA2698727A1 (en) | 2007-09-17 | 2009-03-26 | Affomix Corporation | Increasing specificity in a scfv screen using dual bait reporters |
| US7863425B2 (en) * | 2007-09-26 | 2011-01-04 | Cornell University | Compositions and methods for inhibiting Yersinia pestis infection |
| AU2008311815B2 (en) | 2007-10-19 | 2014-02-06 | Seagen Inc. | CD19 binding agents and uses thereof |
| CA2711806A1 (en) | 2008-01-11 | 2009-07-16 | Morphosys Ag | Display vectors and methods and uses thereof |
| EP2244563B1 (en) * | 2008-02-01 | 2019-08-28 | Bogin, Robert | Methods, compositions, and systems for production of recombinant spider silk polypeptides |
| JP2009225781A (ja) * | 2008-02-29 | 2009-10-08 | Neosilk Co Ltd | カイコを用いた糖鎖構造に特徴を有する糖蛋白質の製造方法 |
| EP2098536A1 (en) | 2008-03-05 | 2009-09-09 | 4-Antibody AG | Isolation and identification of antigen- or ligand-specific binding proteins |
| EP2379718B2 (en) * | 2008-12-16 | 2020-12-30 | Novartis AG | Yeast display systems |
| US20100311116A1 (en) * | 2009-06-04 | 2010-12-09 | Excellgene Sa | Fast generation of high expression stable cell lines expressing recombinant proteins under minimal and short-term selective pressure |
| BR112013014949B1 (pt) | 2010-12-15 | 2021-12-28 | Inter-University Research Institute Corporation Research Organization Of Information And Systems | Métodos para a produção de uma proteína de interesse e para a obtenção de uma suspensão de células cho capaz de sobreviver e proliferar em um meio sem soro que produz uma proteína de interesse |
-
2012
- 2012-07-30 PT PT121785299T patent/PT2692865E/pt unknown
- 2012-07-30 DK DK12178529.9T patent/DK2692865T3/en active
- 2012-07-30 ES ES12178529.9T patent/ES2528892T3/es active Active
- 2012-07-30 SI SI201230139T patent/SI2692865T1/sl unknown
- 2012-07-30 EP EP12178529.9A patent/EP2692865B1/en active Active
-
2013
- 2013-07-18 JP JP2015522101A patent/JP6043431B2/ja active Active
- 2013-07-18 AU AU2013291971A patent/AU2013291971B2/en active Active
- 2013-07-18 WO PCT/EP2013/065214 patent/WO2014013026A1/en active Application Filing
- 2013-07-18 BR BR112015000836-4A patent/BR112015000836B1/pt active IP Right Grant
- 2013-07-18 MX MX2015000885A patent/MX338911B/es active IP Right Grant
- 2013-07-18 CA CA2878982A patent/CA2878982C/en active Active
- 2013-07-18 CN CN201380048308.7A patent/CN104640985B/zh active Active
- 2013-07-18 US US14/414,981 patent/US10174309B2/en active Active
- 2013-07-18 NZ NZ705074A patent/NZ705074A/en unknown
- 2013-07-18 EP EP13177083.6A patent/EP2687599A1/en not_active Withdrawn
- 2013-07-18 SG SG11201500280XA patent/SG11201500280XA/en unknown
-
2015
- 2015-01-13 ZA ZA2015/00203A patent/ZA201500203B/en unknown
- 2015-01-18 IL IL236771A patent/IL236771A/en active IP Right Grant
- 2015-02-17 IN IN1301DEN2015 patent/IN2015DN01301A/en unknown
- 2015-09-08 HK HK15108709.8A patent/HK1209779A1/xx unknown
-
2018
- 2018-10-30 US US16/175,073 patent/US20190153433A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| BR112015000836A2 (pt) | 2017-12-12 |
| AU2013291971A1 (en) | 2015-02-05 |
| CN104640985B (zh) | 2021-05-11 |
| US20150152406A1 (en) | 2015-06-04 |
| EP2692865A1 (en) | 2014-02-05 |
| CA2878982C (en) | 2017-01-03 |
| MX2015000885A (es) | 2015-07-14 |
| AU2013291971B2 (en) | 2016-02-04 |
| SI2692865T1 (sl) | 2015-03-31 |
| PT2692865E (pt) | 2015-02-06 |
| SG11201500280XA (en) | 2015-02-27 |
| JP6043431B2 (ja) | 2016-12-14 |
| WO2014013026A8 (en) | 2014-12-04 |
| DK2692865T3 (en) | 2015-02-16 |
| CN104640985A (zh) | 2015-05-20 |
| ZA201500203B (en) | 2015-12-23 |
| CA2878982A1 (en) | 2014-01-23 |
| ES2528892T3 (es) | 2015-02-13 |
| US20190153433A1 (en) | 2019-05-23 |
| IL236771A (en) | 2016-09-29 |
| EP2687599A1 (en) | 2014-01-22 |
| US10174309B2 (en) | 2019-01-08 |
| EP2692865B1 (en) | 2014-12-10 |
| WO2014013026A1 (en) | 2014-01-23 |
| JP2015522286A (ja) | 2015-08-06 |
| HK1209779A1 (en) | 2016-04-08 |
| BR112015000836B1 (pt) | 2021-12-21 |
| NZ705074A (en) | 2017-04-28 |
| IN2015DN01301A (es) | 2015-07-03 |
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