RU2017139241A - Способ получения клона стволовых клеток, пригодного для индуцирования дифференцировки в соматические клетки. - Google Patents
Способ получения клона стволовых клеток, пригодного для индуцирования дифференцировки в соматические клетки. Download PDFInfo
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- RU2017139241A RU2017139241A RU2017139241A RU2017139241A RU2017139241A RU 2017139241 A RU2017139241 A RU 2017139241A RU 2017139241 A RU2017139241 A RU 2017139241A RU 2017139241 A RU2017139241 A RU 2017139241A RU 2017139241 A RU2017139241 A RU 2017139241A
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- Prior art keywords
- cells
- differentiation
- induction
- stem cells
- somatic cells
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- 210000000130 stem cell Anatomy 0.000 title claims 11
- 230000004069 differentiation Effects 0.000 title claims 10
- 210000001082 somatic cell Anatomy 0.000 title claims 9
- 230000001939 inductive effect Effects 0.000 title claims 3
- 238000004519 manufacturing process Methods 0.000 title claims 2
- 238000000034 method Methods 0.000 claims 11
- 108090000623 proteins and genes Proteins 0.000 claims 10
- 230000006698 induction Effects 0.000 claims 6
- 210000002361 Megakaryocyte Progenitor Cell Anatomy 0.000 claims 3
- 230000032459 dedifferentiation Effects 0.000 claims 2
- 210000003593 megakaryocyte Anatomy 0.000 claims 2
- 101001139134 Homo sapiens Krueppel-like factor 4 Proteins 0.000 claims 1
- 101001094700 Homo sapiens POU domain, class 5, transcription factor 1 Proteins 0.000 claims 1
- 101000713275 Homo sapiens Solute carrier family 22 member 3 Proteins 0.000 claims 1
- 101000687905 Homo sapiens Transcription factor SOX-2 Proteins 0.000 claims 1
- 102100020677 Krueppel-like factor 4 Human genes 0.000 claims 1
- 101100058550 Mus musculus Bmi1 gene Proteins 0.000 claims 1
- 108091057508 Myc family Proteins 0.000 claims 1
- 108700020796 Oncogene Proteins 0.000 claims 1
- 102000043276 Oncogene Human genes 0.000 claims 1
- 102100035423 POU domain, class 5, transcription factor 1 Human genes 0.000 claims 1
- 102100023075 Protein Niban 2 Human genes 0.000 claims 1
- 108700025695 Suppressor Genes Proteins 0.000 claims 1
- 102100024270 Transcription factor SOX-2 Human genes 0.000 claims 1
- 101100022813 Zea mays MEG3 gene Proteins 0.000 claims 1
- 230000006907 apoptotic process Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 210000001612 chondrocyte Anatomy 0.000 claims 1
- 210000000349 chromosome Anatomy 0.000 claims 1
- 238000010367 cloning Methods 0.000 claims 1
- 230000002950 deficient Effects 0.000 claims 1
- 239000003814 drug Substances 0.000 claims 1
- 229940079593 drug Drugs 0.000 claims 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims 1
- 210000003494 hepatocyte Anatomy 0.000 claims 1
- 230000035800 maturation Effects 0.000 claims 1
- 210000002752 melanocyte Anatomy 0.000 claims 1
- 230000002107 myocardial effect Effects 0.000 claims 1
- 210000001982 neural crest cell Anatomy 0.000 claims 1
- 210000001178 neural stem cell Anatomy 0.000 claims 1
- 210000002569 neuron Anatomy 0.000 claims 1
- 210000003924 normoblast Anatomy 0.000 claims 1
- 108700042657 p16 Genes Proteins 0.000 claims 1
- 230000008672 reprogramming Effects 0.000 claims 1
- 210000002363 skeletal muscle cell Anatomy 0.000 claims 1
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- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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Claims (17)
1. Способ получения клона стволовых клеток, который содержит этапы:
(i) введения в стволовые клетки экзогенного гена, связанного с индукцией дифференцировки в соматические клетки;
(ii) индукции дифференцировки стволовых клеток, в которые введен экзогенный ген, в соматические клетки;
(iii) дедифференцировки индуцированных к дифференцировке соматических клеток; и
(iv) выделения стволовых клеток, несущих экзогенный ген, встроенный в их хромосому, из колонии стволовых клеток, образованных на этапе (iii).
2. Способ по п. 1, где эффективность индуцирования дифференцировки изолированных клонов стволовых клеток в соматические клетки выше по сравнению с эффективностью индуцирования дифференцировки стволовых клеток перед клонированием.
3. Способ по п. 1, где соматическими клетками являются гемопоэтические клетки-предшественники, клетки-предшественники мегакариоцитов, эритробласты, нервные клетки, нейральные стволовые клетки, клетки нервного гребня, миокардиальные клетки, клетки скелетных мышц, хондроциты, гепатоциты или меланоциты.
4. Способ по п. 3, где соматическими клетками являются клетки-предшественники мегакариоцитов, и экзогенный ген, связанный с индукцией дифференцировки является, по меньшей мере, одним экзогенным геном, выбранным из группы, состоящей из онкогенов, включая гены семейства MYC, гены (гены поликомб), ингибирующие экспрессию гена p16 или гена p19, включая Bmi1, и гены, подавляющие апоптоз, включая ген BCL-XL.
5. Способ в соответствии с п.1, где изолируют стволовые клетки, экспрессирующие MEG3.
6. Способ в соответствии с п.1, где экзогенный ген, связанный с индукцией дифференцировки, функционально связан с отвечающим на препарат промотором.
7. Способ в соответствии с п.1, где дедифференцировку на этапе (iii) проводят посредством введения репрограммирующего фактора, выбранного из группы, состоящей из OCT3/4, SOX2 и KLF4.
8. Способ получения соматических клеток, который содержит этап:
индукции дифференцировки клонов стволовых клеток, полученных в соответствии со способом в соответствии с п.1, в соматические клетки.
9. Способ получения тромбоцитов, который содержит этапы:
индукции дифференцировки клонов стволовых клеток, полученных в соответствии со способом в соответствии п.1 в клетки-предшественники мегакариоцитов; и
обеспечение созревания индуцированных к дифференцировке клеток-предшественников мегакариоцитов в мегакариоциты и высвобождения тромбоцитов.
10. Способ по п. 9, где полученные тромбоциты являются дефицитными по HLA.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015-082768 | 2015-04-14 | ||
| JP2015082768 | 2015-04-14 | ||
| PCT/JP2016/062040 WO2016167329A1 (ja) | 2015-04-14 | 2016-04-14 | 体細胞への分化誘導に適した幹細胞クローンを製造する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2017139241A true RU2017139241A (ru) | 2019-05-14 |
| RU2017139241A3 RU2017139241A3 (ru) | 2019-09-16 |
| RU2730034C2 RU2730034C2 (ru) | 2020-08-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2017139241A RU2730034C2 (ru) | 2015-04-14 | 2016-04-14 | Способ получения клона стволовых клеток, пригодного для индуцирования дифференцировки в соматические клетки |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US20180051260A1 (ru) |
| EP (1) | EP3296390B1 (ru) |
| JP (1) | JP6873898B2 (ru) |
| KR (1) | KR102563564B1 (ru) |
| CN (1) | CN107709543B (ru) |
| AU (1) | AU2016248858A1 (ru) |
| BR (1) | BR112017022219A2 (ru) |
| CA (1) | CA2982568A1 (ru) |
| IL (1) | IL255031B (ru) |
| RU (1) | RU2730034C2 (ru) |
| SG (1) | SG11201708395VA (ru) |
| WO (1) | WO2016167329A1 (ru) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108866005A (zh) * | 2018-07-20 | 2018-11-23 | 东南大学 | 一种永生化人源神经干细胞系及其制备方法 |
| WO2024143555A1 (ja) * | 2022-12-28 | 2024-07-04 | 国立大学法人千葉大学 | 細胞分化度の調節方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2632142C (en) * | 2005-12-13 | 2013-08-06 | Kyoto University | Nuclear reprogramming factor |
| US20120034192A1 (en) * | 2008-09-19 | 2012-02-09 | Young Richard A | Compositions and methods for enhancing cell reprogramming |
| SG10201600234PA (en) * | 2008-10-24 | 2016-02-26 | Wisconsin Alumni Res Found | Pluripotent Stem Cells Obtained By Non-Viral Reprogramming |
| CN102712906B (zh) * | 2009-09-15 | 2015-09-30 | 国立大学法人东京大学 | 分化细胞的新型制备方法 |
| US20120231490A1 (en) * | 2009-10-28 | 2012-09-13 | Japan Health Sciences Foundation | Method Of Differentiation From Stem Cells To Hepatocytes |
| WO2011096482A1 (ja) * | 2010-02-03 | 2011-08-11 | 国立大学法人東京大学 | 多能性幹細胞を用いた免疫機能再建法 |
| US20120009158A1 (en) * | 2010-06-18 | 2012-01-12 | The General Hospital Corporation | VENTRICULAR INDUCED PLURIPOTENT STEM (ViPS) CELLS FOR GENERATION OF AUTOLOGOUS VENTRICULAR CARDIOMYOCYTES AND USES THEREOF |
| WO2012157586A1 (ja) * | 2011-05-13 | 2012-11-22 | 国立大学法人東京大学 | 多核化巨核球細胞、及び血小板の製造方法 |
| JP6143265B2 (ja) * | 2011-07-25 | 2017-06-07 | 国立大学法人京都大学 | 人工多能性幹細胞の選別方法 |
| JP2013240308A (ja) * | 2012-05-23 | 2013-12-05 | Kagoshima Univ | ヒトＥＳ／ｉＰＳ細胞における遺伝子発現方法 |
| US20160002599A1 (en) * | 2013-02-08 | 2016-01-07 | Kyoto University | Production methods for megakaryocytes and platelets |
| WO2014148646A1 (ja) | 2013-03-21 | 2014-09-25 | 国立大学法人京都大学 | 神経分化誘導用の多能性幹細胞 |
-
2016
- 2016-04-14 JP JP2017512584A patent/JP6873898B2/ja active Active
- 2016-04-14 CN CN201680034787.0A patent/CN107709543B/zh active Active
- 2016-04-14 SG SG11201708395VA patent/SG11201708395VA/en unknown
- 2016-04-14 AU AU2016248858A patent/AU2016248858A1/en not_active Abandoned
- 2016-04-14 KR KR1020177032755A patent/KR102563564B1/ko active IP Right Grant
- 2016-04-14 BR BR112017022219A patent/BR112017022219A2/pt not_active Application Discontinuation
- 2016-04-14 RU RU2017139241A patent/RU2730034C2/ru active
- 2016-04-14 EP EP16780120.8A patent/EP3296390B1/en active Active
- 2016-04-14 WO PCT/JP2016/062040 patent/WO2016167329A1/ja active Application Filing
- 2016-04-14 US US15/565,922 patent/US20180051260A1/en not_active Abandoned
- 2016-04-14 CA CA2982568A patent/CA2982568A1/en not_active Abandoned
-
2017
- 2017-10-15 IL IL255031A patent/IL255031B/en unknown
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2020
- 2020-04-03 US US16/839,906 patent/US20210062158A1/en not_active Abandoned
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2023
- 2023-09-27 US US18/476,065 patent/US20240026304A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR20180019519A (ko) | 2018-02-26 |
| RU2017139241A3 (ru) | 2019-09-16 |
| RU2730034C2 (ru) | 2020-08-14 |
| US20180051260A1 (en) | 2018-02-22 |
| JP6873898B2 (ja) | 2021-05-19 |
| KR102563564B1 (ko) | 2023-08-07 |
| IL255031A0 (en) | 2017-12-31 |
| CN107709543B (zh) | 2021-11-09 |
| CA2982568A1 (en) | 2016-10-20 |
| CN107709543A (zh) | 2018-02-16 |
| AU2016248858A1 (en) | 2017-11-09 |
| IL255031B (en) | 2022-04-01 |
| US20240026304A1 (en) | 2024-01-25 |
| EP3296390A1 (en) | 2018-03-21 |
| EP3296390B1 (en) | 2023-01-04 |
| WO2016167329A1 (ja) | 2016-10-20 |
| JPWO2016167329A1 (ja) | 2018-02-15 |
| EP3296390A4 (en) | 2019-01-09 |
| BR112017022219A2 (pt) | 2018-07-31 |
| US20210062158A1 (en) | 2021-03-04 |
| SG11201708395VA (en) | 2017-11-29 |
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