KR20130140390A - Natural germicide composition and methods for manufacturing a natural germicide - Google Patents

Abstract

The present invention relates to a germicide composition containing natural materials as active ingredients and a production method of the same, specifically a natural germicide composition having sterilization activity and antifungal functions against gram-positive bacteria, gram-negative bacteria, and mycete. The natural germicide composition contains 95-100 parts by weight of ginseng extract, 95-100 parts by weight of garlic extract, 190-200 parts by weight of white cucumber extract, 48-50 parts by weight of Maximowiczia typical fruit extract, and 48-50 parts by weight of Chinese pepper extract as active ingredients.

Description

Natural germicide composition and methods for manufacturing a natural germicide

The present invention relates to a fungicide composition containing a natural substance as an active ingredient and a method for preparing the same, and more particularly, to a natural fungicide composition having bactericidal activity and antifungal action against Gram-positive bacteria, Gram-negative bacteria and fungi.

Generally, viruses such as influenza, avian influenza, new influenza, foot-and-mouth disease, gram-positive bacteria such as staphylococci, streptococci, pneumococci, diphtheria bacteria, tetanus bacteria, anthrax bacteria, actinomycetes, Salmonella bacteria, dysentery bacteria, typhoid bacteria, E. coli bacteria, cholera bacteria, etc. Gram-negative bacteria are infected by the human body through respiratory or conjunctival or skin contact, causing various diseases such as pneumonia, typhoid fever, paratyphoid, conjunctivitis, food poisoning, cholera, and dysfunction of whole organs, especially high risk groups such as children, the elderly, chronic diseases Infection and transmission can be fatal, resulting in increased morbidity and mortality.

Recently, as the incidence of various diseases caused by such viruses and bacteria increases, hygiene concepts for personal hygiene and food have been highlighted, and fungicides and disinfectants for various uses have been released, but most of them are ethanol, alcohol compounds, glutaraldehyde compounds, It is composed of synthetic chemicals such as chlorine and formalin, so it has high toxicity and resistance, which can cause harmful side effects and secondary environmental pollution.

Meanwhile, fungal diseases that occur in crops or ornamental plants such as powdery mildew and gray mold are one of the major plant diseases that lower the productivity of the crops. Synthetic fungicides are used to control pathogens of fungal diseases, but synthetic fungicides cause serious problems such as environmental pollution, resistant bacteria, and residual toxicity due to misuse.

As a result, it is not only to increase the mortality rate of various pathogens, but also to be decomposed well when exposed to the environment with little toxicity, and it is eager for the development of environment-friendly natural disinfectants and insect repellents. There is a situation.

Republic of Korea Patent Publication No. 10-2011-0029544 (2011.03.23) Republic of Korea Patent Publication No. 10-1140147 (2012.05.02) Republic of Korea Patent Publication No. 10-1095893 (2011.12.28) Republic of Korea Patent Publication No. 10-2011-0022029 (2011.03.04) Republic of Korea Patent Publication No. 10-2012-0045851 (2012.05.09) Republic of Korea Patent Publication No. 10-2012-0042009 (2012.05.03) Republic of Korea Patent Publication No. 10-2011-0080720 (2011.07.13) Republic of Korea Patent Publication No. 10-2011-0067841 (2011.06.22) Republic of Korea Patent Publication No. 10-0961879 (2010.05.31) Republic of Korea Patent Publication No. 10-0947282 (2010.03.05) Republic of Korea Patent Publication No. 10-1130690 (2012.03.20) Republic of Korea Patent Publication No. 10-1098304 (2011.12.19) Republic of Korea Patent Publication No. 10-2009-0096306 (2009.09.10) Republic of Korea Patent Publication No. 10-1079491 (2011.10.27) Republic of Korea Patent Publication No. 10-0860785 (2008.09.23) Republic of Korea Patent Publication No. 10-2009-0102378 (2009.09.30) Republic of Korea Patent Publication No. 10-2009-0057591 (2009.06.08) Republic of Korea Patent Publication No. 10-0853717 (2008.08.18) Republic of Korea Patent Publication No. 10-2008-0011847 (2008.02.11) Republic of Korea Patent Publication No. 10-0701585 (2007.03.23)

Therefore, the present inventors focus on solving the above-mentioned matters and problems, and select natural substances having excellent bactericidal, antimicrobial and deodorizing properties and contain them as active ingredients, which are harmless to the human body and are environmentally friendly, and also kill various bacteria and viruses. Exercising the strong sterilizing power to make a natural disinfectant composition that can be used for a wide range of applications, such as fungicides, antibacterial, antifungal, disinfectant, insect repellent has been invented and completed the invention of the present invention for many years.

Therefore, it is an object of the present invention to provide a natural disinfectant composition and a method for producing the same, which are harmless to the human body, environmentally friendly, and which can be used for various uses.

Preferred embodiments of the present invention to achieve the above object, ginseng extract 95-100 parts by weight, garlic extract 95-100 parts by weight, encyclopedia 190-200 parts by weight, Schizandra extract 48-50 parts by weight and acid It provides a natural fungicide composition containing 48 ~ 50 parts by weight of the extract as an active ingredient.

As a result, the present invention is harmless to the human body, is environmentally friendly, and has excellent sterilization and antifungal effects, so that the present invention can be efficiently used for various applications.

In another embodiment of the present invention, mugwort extract 48-50 parts by weight, poongyoung extract 48-50 parts by weight, ginger extract 48-50 parts by weight, reef extract 48-50 parts by weight, cornus extract 48-50 parts by weight, plum extract 48 to 50 parts by weight and pomegranate extract 48 to 50 parts by weight may be further included at least one selected from the group consisting of.

Here, each of the extracts is preferably made of a distillate to extract the decoction.

And another embodiment of this invention, (A) 95-100 weight part of ginseng, 95-100 weight part of garlic, 190-200 weight part of encyclopedia, 48-50 weight part of Schisandra chinensis, 48-50 weight part of Sancho, 34-36 parts Fermenting at 70 ° C. for 70-72 hours (B) Drying each of the components passed through step (A) at 50-60 ° C. for 46-50 hours (C) Each component having passed step (B) Mixing with 1000 parts by weight of ethyl alcohol and aged at 75-80 ° C. for 22-24 hours (D) decoupling the mixture passed through step (C) at 100 ° C. for 120-150 minutes (E) the (D) It provides a method for producing a natural disinfectant composition comprising the step of extracting the distillate while further stirring the mixture through the step at 100 ° C for 7-8 hours.

Another embodiment of the present invention, (A) 95-100 parts by weight of ginseng, 95-100 parts by weight of garlic, 190-200 parts by weight of encyclopaedia, 48-50 parts by weight of Schizandra chinensis, 48-50 parts by weight of Sancho, 34-36 ° C Fermenting for 70 to 72 hours at (B) drying each of the components passed through step (A) at 50 to 60 ° C. for 46 to 50 hours (C) constituting each of the components passed through step (B) Mixing with 1000 parts by weight of ethyl alcohol and aging for 22-24 hours at 75-80 ° C. (D) decoupling the mixture passed through step (C) at 100 ° C. for 120-150 minutes (E) (D) It is also possible to provide a method for producing a natural disinfectant composition comprising the step of extracting the decoction solution by filtration and concentrated under reduced pressure at 30 ~ 60 ℃ the mixture subjected to the step.

In another embodiment of the present invention, 48 to 50 parts by weight of mugwort in the step (A), 48 to 50 parts by weight of poongyoung, 48 to 50 parts by weight of ginger, 48 to 50 parts by weight of poncho, 48 to 50 parts by weight of cornus oil, 48 to 50 parts by weight of plum and 48 to 50 parts by weight of pomegranate may further provide a method for producing a natural disinfectant composition further containing at least one.

The present invention having a solution and a solution of the above problems has excellent bactericidal activity and antifungal action against Gram-positive bacteria, Gram-negative bacteria and fungi, thus exhibiting a strong bactericidal power to kill various bacteria and viruses, including fungicides, antibacterial agents, antifungal agents, It can be used efficiently for a wide range of applications such as disinfectants and insect repellents, and since it is made of natural materials, it has no harmful toxicity and is well decomposed when exposed to the environment, which is environmentally friendly, and has excellent safety for humans, livestock and crops.

1 is a test report showing the results of the sterilization and antifungal test for Example 1 of the present invention,
Figure 2 is a test report showing the results of the bactericidal and antifungal test for Example 2 of the present invention,
3 to 5 is a test report showing the results of the sterilization and antifungal test for Example 3 of the present invention,

Hereinafter, embodiments according to the present invention will be described more specifically with reference to the accompanying drawings.

Before describing the present invention, it is to be understood that the following terms are defined in consideration of the functions of the present invention, and that they should be construed in accordance with the technical idea of the present invention and interpreted in a general sense or commonly understood in the technical field.

In the following description, well-known functions or constructions are not described in detail to avoid obscuring the subject matter of the present invention.

Natural disinfectant composition according to an embodiment of the present invention is largely comprised of ginseng, garlic, encyclopedia, Schizandra chinensis and extract extracted from the Japanese pepper in a specific ratio.

Ginseng (Panax ginseng C.A Meyer) is known to have a wide variety of effects in addition to central nervous control. For example, wound healing by epidermal cell proliferation of ginsenoside Rb2 contained in ginseng (Arch. Pharm. Res. 2002 Feb; 25 (1): 71-6), cancer prevention effect of ginseng extracts and derivatives (J. Med. Sci. 2001 Dec; 16 Suppl: S19-27), alleviating the effects of wounds and inflammation on excessive muscle spasms (Comp. Biochem. Physiol. C Toxicol. Pharmacol. 2001 Nov; 130 (3): 369-77) , Cell growth promoting effect of ginseng extract (US Pat. No. 5,318,906), Effect of ginseng aqueous solution on cell proliferation and apoptosis (Kin SH, In Vivo 1998 Mar-Apr; 12 (2): 219-22) And anti-inflammatory effects of ginseng (Planta. Med. 1990, 56 (1), 19-23).

Ginsenosides, polysaccharides, peptides, polyacetyl, flavonoids, fatty acids and the like are active ingredients in ginseng. In particular, ginsenosides have antioxidant functions that have many physiological activities against anticancer and immune functions (Wu, Gardner, Murphy, Seals, Kensil, Recchia, Beltz, Newman, & Newman, 1992; Ryu, Oak & Kim, 2000 Shibata, 2001).

In addition, ginsenosides and polyacetyls (panassacol, panassidol, panascitriol, etc.) have been reported to have antimicrobial activity against both Gram-positive and Gram-negative food poisoning bacteria (Sung & Lee, 2008). Acidic polysaccharides isolated from ginseng have been reported to inhibit Staphylococcus aureus (Benny & Vanitha, 2004).

Garlic is composed of capsaicin, allicin, organic sulfur, etc., and has antibacterial, antiviral, and antifungal functions. The antimicrobial activity of garlic extracts does not fall significantly compared to other antimicrobial agents. It has been reported that sterilization and disinfection of various bacteria such as dysentery, typhoid bacteria, and microorganisms are unlikely to be resistant to antibiotics, given antibiotics.

Also, in the garlic extract, cholera, vibrio, staphylococci do not grow, and fungi do not grow. In addition, typhoid bacteria kill in 5 minutes and have a stronger bactericidal activity than antibiotics such as penicillin and theramycin.

Encyclopedia is a genus of Ginkgo biloba Linnaeus, a deciduous tree of Ginkgo biloba, whose main components are protein, fat, starch, Ginnol, Ginkgolic acid, and Hydroginkgolinic acid. Encyclopedia contains a lot of ganol, pectin, histidine, starch, protein, fat, sugar, etc., long-term eating for patients with pulmonary tuberculosis or asthma, less cough and less phlegm. This effect is because the encyclopaedia active respiratory function, extinguish inflammation and inhibit the development of Mycobacterium tuberculosis.

In addition, one of the peculiar effects of encyclopaedia is the composition of lecithin and elgosterin, which is the parent of vitamin B, which has effects on improving cerebrovascular effects such as decreased libido, cerebral anemia, nervous breakdown, and systemic fatigue.

Sancho is the skin of Zanthoxylum piperitum De Candolle or the same plant, which is effective for indigestion, planting, gastric and sewage, gastric dilatation, vomiting, dysentery, diarrhea, nervous breakdown, cough, and roundworm control. It is effective for abdominal pain, diarrhea and toothache, asthma and low back pain caused by cold energy of the abdomen, and also has insecticidal action for scabies, ringworm, genital itching, scrotum eczema, etc. Antimicrobial activity has been reported.

Omija (五味子) is a dried medicinal herb of Schisandra chinensis Baillon, which is effective for seawater and asthma. Pharmacological action: cerebral cortex excitation, blood pressure lowering, expectoration, antitussive action, respiratory excitability, cardiac action, glucose metabolism and glycolysis, cellular immune function enhancement, uterine excitability, bile secretion, gastric juice secretion control Action, fungal action has been reported.

Mugwort contains active ingredients such as cineol, choline, adenine, monoginine, eucalyptol and artemisine.

Chinenol (chneol) is a natural organic compound used for the treatment of chronic obstructive pulmonary disease and asthma. It can prevent respiratory diseases caused by turbid air, has antiseptic effect and antiseptic effect, and improves immunity.

Choline (choline) is one of the vitamin B complex has the effect of preventing fatty liver (脂肪 肝).

Adenine (Adenine) has a basic, nucleic acid ADP, FMN, NAD, NADP, COA, etc. react in vivo to prevent disease and have immunity.

Monoginine has detoxification and hemostatic effects.

In addition, Eucalyppol has a unique aroma and refreshing taste, and Artemisine has a strong antibacterial ability to kill malaria. In particular, it is known to have effects such as strong blood clotting, detoxification, antibacterial, tonic, gangjeong, anti-inflammatory, analgesic, immunity, diuresis, hemostasis, and appetite in the human body or animals.

In addition, the fragrance of eucalyppol has the effect of killing or inhibiting proliferation of Staphylococcus aureus, hemolytic streptococci, Escherichia coli, diphtheria bacteria, and malaria bacteria.

Pogongyoung is a rooted outpost of Taraxacum platycarpum, Taraxacum mongolicum, Taraxacum sinicum, and Taraxacum coreanum, which has the effect of lowering and detoxifying heat, inhibiting the activity of inflammatory enzymes and releasing coldness. Acute conjunctivitis, cold fever and acute tonsillitis are used for treatment.

Ginger (Zingiber officinale ROSC.) Treats chills, fever, headaches, vomiting, seawater and phlegm from colds, and is effective for abdominal pain and diarrhea caused by food poisoning. Pharmacological actions have been reported to increase metabolic function, promote gastric juice secretion, enhance digestion, cardiac excitement, promote blood circulation, and inhibit fungi.

Reef (dried before the ripening of pepper) is the main ingredient, piperine (5 ~ 5.5%), chavicine (6%) and essential oils 1 ~ 2.5%, It is known to promote digestion and treat indigestion, food insufficiency and diarrhea caused by weak stomach.

Cornus (cornin), moroniside (Morroniside), loganin (tanan), tannin (saponin), and glycosides such as saponin and organic acids such as acid, malic acid, tartaric acid, etc. Vitamin A, a large amount of sugar and palmitic acid, oleic acid, linoleic acid and the like is contained. It is used as a medicine for headache, tinnitus, seawater disease, fever, and excessive menstruation. It has effects such as cold and cold.

Plums are rich in minerals, vitamins, organic acids (citric acid, apple acid, pumpkin, tartaric acid, catechin acid), and also contain carotene and minerals such as calcium, phosphorus, and potassium. Among them, citric acid promotes the metabolism of sugar and relieves fatigue, organic acid activates the gastrointestinal action and stimulates appetite, and catechin acid inhibits the growth of harmful bacteria in the intestine.

In addition, alkaline foods are good for fatigue recovery and have a constitution improvement effect. Especially excellent detoxification function helps to treat stomach and food poisoning, and acidity secretes gastric juice and normalizes digestive organs to eliminate digestive and gastrointestinal disorders. Good for constipation and skin beauty, high acidity and has a powerful sterilizing action. Recently, it was also known as anticancer food.

Pomegranate (石榴) is rich in estrogen has the effect of reducing various symptoms. Pomegranate seeds contain a large amount of polyphenols and fatty acids (punicic acid, palmitic acid, stearic acid, oleic acid, linoleic acid), and pomegranate fruit contains a lot of polyphenols and anthocyanin to help antioxidant function and cardiovascular health. Gives.

Both fruit and bark are good for preventing hypertension and atherosclerosis, and are effective for women's diseases and boosted swelling. Particularly when the dysentery is excellent, the volatile alkaloids are used to parasite, tapeworm remedies used.

Ache is a tuber of Curcuma phaeocaulis, Curcuma kwangsiensis, Curcuma wenyujin, Curcuma zedoaria, and has the effect of bleeding, removing blood, helping circulation, and pain It is known to have an antibacterial action and an effect of inhibiting and destroying cancer cells.

Creation is a medicinal herb made from root stems of Atractylodes japonica KOIDZ. Et.KITAGAWA, A. lancea DC., And A. chinensis KOIDZ. , The active ingredient contains 5-9% of essential oils, the main ingredients include atacticol (Atractylol), hinsol (Hinesol), beta- Eudesmol (β-Eudesmol), sweating, diuresis, analgesic, stomach, etc. Efficacy in anorexia, indigestion, gastroenteritis, colds, etc., and has the effect of suppressing the pathogen and increase the resistance.

Example 1 Preparation of a Distillation Natural Disinfectant Composition

Under the conditions of specific temperature and time during the manufacturing process as follows, it is possible to obtain a natural disinfectant composition having a special ingredient and efficacy.

1) Fermentation

100 parts by weight of ginseng, 100 parts by weight of garlic, 200 parts by weight, 50 parts by weight of schizandrae, 50 parts by weight of vinegar, which have been washed cleanly to remove impurities such as dust and foreign substances on the surface, and then cut or sorted to a predetermined size 50 parts by weight of wormwood and mugwort were put into a fermentor and fermented at 35 ° C. for 72 hours.

2) dry

Each component that had undergone the fermentation step was placed in a dryer and blow dried at 60 ° C. for 48 hours to remove moisture.

3) ripening

Each component, which had undergone the drying step, was mixed with 1000 ml of ethyl alcohol, put into a aging machine, and aged at 80 ° C. for 24 hours.

4) heating

The mixture passed through the aging step was placed in a shaker and decoction at 100 ° C. for 120 minutes.

5) Distillate Extraction

The decanted mixture was further dehydrated at 100 ° C. for 8 hours, and the distillate generated was extracted to obtain a natural disinfectant composition.

≪ Example 2 > Concentrated  Preparation of Natural Disinfectant Composition

Under the conditions of specific temperature and time during the manufacturing process as follows, it is possible to obtain a natural disinfectant composition having a special ingredient and efficacy.

1) Fermentation

95 parts by weight of ginseng, 95 parts by weight of garlic, white parts of 190 parts by weight, 48 parts by weight of schizandrae and 48 parts of vinegar by washing cleanly to remove impurities such as dust or foreign substances on the surface and then cutting or sorting them to a predetermined size The part was put into a rice tray and put into a fermenter, and the fermentation was carried out at 35 ° C. for 72 hours.

2) dry

Each component that had undergone the fermentation step was placed in a dryer and blow dried at 60 ° C. for 48 hours to remove moisture.

3) ripening

Each component, which had undergone the drying step, was mixed with 1000 ml of ethyl alcohol, put into a aging machine, and aged at 75 ° C. for 24 hours.

4) heating

The mixture, which had undergone the fermentation step, was placed in a shaker and sweetened at 100 ° C. for 120 minutes.

5) Concentrate Extraction

Remove the impurities such as residues through the purification and filtration process to put the mixture through the funnel into the funnel, and then put it in a vacuum condenser and concentrated to 1/10 of the original volume at 30 ~ 60 ℃ natural disinfectant composition Got.

Example 3 Preparation of a Distillation Type Natural Disinfectant Composition

Under the conditions of specific temperature and time during the manufacturing process as follows, it is possible to obtain a natural disinfectant composition having a special ingredient and efficacy.

1) Fermentation

100 parts by weight of ginseng, 100 parts by weight of garlic, 100 parts by weight of garlic, 50 parts by weight of white fruit, 50 parts by weight of omija, and 50 weight of vinegar after washing cleanly to remove impurities such as dust and foreign substances on the surface and then cut or sort them to a predetermined size The part was put into a rice tray and put into a fermenter, and aged at 35 ° C. for 72 hours.

2) dry

Each component that had undergone the fermentation step was placed in a dryer and blow dried at 60 ° C. for 48 hours to remove moisture.

3) ripening

Each component, which had undergone the drying step, was mixed with 1000 ml of ethyl alcohol, put into a aging machine, and fermented at 80 ° C. for 24 hours.

4) heating

The mixture, which had undergone the fermentation step, was placed in a shaker and sweetened at 100 ° C. for 120 minutes.

5) Distillate Extraction

The decanted mixture was further dehydrated at 100 ° C. for 8 hours, and the distillate generated was extracted to obtain a natural disinfectant composition.

The natural disinfectant composition obtained by the above method is not toxic to human body, animal, environment, etc., and it is a disinfectant, disinfectant, which sterilizes and controls various harmful pathogens when sprayed on the surface of an object suspected of microbial infection or on skin or crop of human body. Effectively and stably exhibit the functionality as an antibacterial agent, insect repellent and deodorant to remove odors.

In addition, the natural disinfectant composition according to the present invention can be used as a prophylactic and therapeutic agent for inhibiting bacterial infection by inhibiting bacteria or fungi, as well as replacing agricultural pesticides, reducing intestinal pathogens in livestock to reduce mortality and increase the growth rate. It can improve the breeding environment in the barn by minimizing the generation of various harmful gases during the decomposition of manure.

Comparative Example 1 Preparation of a Distillation Type Natural Disinfectant Composition

A natural fungicide composition was prepared in the same manner as in Example 1. However, the composition ratio of each component was adjusted to 80 parts by weight of ginseng, 80 parts by weight of garlic, 100 parts by weight of white fruit, 30 parts by weight of Schizandra chinensis, 30 parts by weight of Sancho and 30 parts by weight of mugwort.

≪ Comparative Example 2 & Concentrated  Preparation of Natural Disinfectant Composition

A natural bactericide composition was prepared in the same manner as in Example 2. However, the composition ratio of each component was adjusted to 110 parts by weight of ginseng, 110 parts by weight of garlic, 220 parts by weight of white fruit, 60 parts by weight of Schizandra chinensis, and 60 parts by weight of Sancho.

Test Example 1 Test of bactericidal and antifungal efficacy against Example 1

Example 1 A natural fungicide composition for antimicrobial and antifungal effect of the gram-positive methicillin-resistant Staphylococcus aureus to measure the prepared by (Methicillin - Resistant Staphyllococcus Aureus: MRSA ) ATCC 33591, (S. mutans) of ATCC 27351, mujomgyun Trichophyton rubrum ) KCTC 6345, a test for dandruff, Pityrosporum ovale ATCC 12078, was commissioned by the Korea Institute of Chemical Convergence Testing.

1) Sterilization test method

Adjusting the MRSA and S. mutans strain to the initial number of bacteria 10 ⁵ ~ 10 ⁶ CFU / mL by diluting the cell culture was incubated in liquid medium and was used in the test.

After the test solution was added to 20 mL of the test solution (the natural bactericide composition obtained in Example 1) and mixed, the bacterial count was measured when the sample was left at room temperature for 48 hours. Bacterial counts were measured as physiological saline, and the initial bacterial counts were expressed.

However, in the initial dilution step, the test was carried out by neutralizing using D / E Neutralizing Broth (DIFCO) .In the case of growth of bacteria in the medium, the number of bacteria on the medium was multiplied by the dilution factor. In the case of non-proliferation, it was expressed as "less than 10 (<10)" by multiplying the dilution factor made in the neutralization step.

Bacterial counts were measured at all stages using TSA (see Section 2.2), and the percent reduction in sterilization was calculated from the relationship of {(AB) / A} × 100. Here, A is the initial number of bacteria, B is the number of bacteria after a certain time.

2) Anti-fungal test method (Halo test)

① Trichophyton rubrum

Trichophyton on Sabouraud Dextrose Agar Spore solution was prepared from rubrum and used for testing.

After incubating 200 μL of the spore suspension in the prepared plate medium (Sabouraud Dextrose Agar), treating 20 μL of the test solution to the sterilized disc (Φ 6.0.mm), and placing the disc in the center of the medium, 10 days at 25 ± 1 ° C. After incubation for a while the diameter of the inhibition zone (Inhibition Zone) was measured.

② Pityrosporum ovale

Pityrosporum on pre-prepared flat media Inoculated with ovale bacteria and enriched for 48 hours at 37 ℃ was used in the test. Well-grown Pityrosporum After ovale bacteria were suspended in sterile saline solution, a 10 ⁸ CFU / mL concentration of bacteria was prepared, inoculated evenly on the prepared medium using a sterile swab, and 20 μL of the test solution was treated to a sterile disc (Φ 6.0.mm). After placing the disc in the center of the medium, the culture was incubated at 35 ± 1 ° C. for 48 hours and the diameter of the inhibition zone was measured. Growth inhibition zone (W) was calculated from the relationship of (TD) / 2. Where W is the width of the low zone (mm), T is the total diameter of the test solution and the low zone (mm), and D is the diameter of the test solution and disc (mm).

3) Test results

The test results are as shown in Table 1 and Table 2 below and FIG. 1.

Sterilization Test Test strain Early 48 hours later Sterilization Reduction Rate MRSA 6.5 × 10 ⁵ <10 99.9% or more S. mutans 2.7 × 10 ⁵ <10 99.9% or more

As shown in Table 1 and Figure 1, the natural bactericidal composition according to Example 1 of the present invention, after the sterilization test for MRSA initial inoculation number after 48 hours at [6.5 × 10 ⁵ CFU / mL] [<10 ( Less than 10) CFU / mL].

In addition, as a result of the sterilization test for S. mutans , the initial inoculum was reduced from [2.7 × 10 ⁵ CFU / mL] to [<10 (less than 10) CFU / mL] after 48 hours.

Antifungal Test Test strain Overall diameter (T) Samples and Disc Diameter (D) Lowland width (W) Trichophyton rubrum 6.0 6.0 0 Pityrosporum ovale 6.0 6.0 0

As shown in Table 2 and Figure 1, the natural disinfectant composition according to Example 1 of the present invention, the result of measuring the size of the inhibition zone (Inhibition Zone), T. rubrum And did not form lowlands for P. ovale .

Test Example 2 Test of bactericidal and antifungal efficacy against Example 2

Example 2 of the methicillin-resistant Gram-positive bacteria to measure the bactericidal and antifungal effects of the natural Staphylococcus aureus fungicide composition produced by (Methicillin - Resistant Staphyllococcus Aureus: MRSA ) ATCC 33591, (S. mutans) of ATCC 27351, mujomgyun Trichophyton rubrum ) KCTC 6345, a test for dandruff, Pityrosporum ovale ATCC 12078, was commissioned by the Korea Institute of Chemical Convergence Testing.

Here, the bactericidal test and the antifungal test (Halo test) was carried out in the same manner as in Test Example 1 described above.

The test results are as shown in Tables 3 and 4 and FIG. 2 below.

Sterilization Test Test strain Early 48 hours later Sterilization Reduction Rate MRSA 6.5 × 10 ⁵ 2.2 x 10 ⁵ 66.2% S. mutans 2.7 × 10 ⁵ <10 99.9% or more

As shown in Table 3 and Figure 2, the natural disinfectant composition according to Example 1 of the present invention, the initial inoculation bacterium count for MRSA after 48 hours at [6.5 × 10 ⁵ CFU / mL] [2.2 × 10 ⁵ CFU / mL].

In addition, as a result of the sterilization test for S. mutans , the initial inoculum was reduced from [2.7 × 10 ⁵ CFU / mL] to [<10 (less than 10) CFU / mL] after 48 hours.

Antifungal Test Test strain Overall diameter (T) Samples and Disc Diameter (D) Lowland width (W) Trichophyton rubrum 6.0 6.0 0 Pityrosporum ovale 6.0 6.0 0

As shown in Table 4 and Figure 2, the natural disinfectant composition according to Example 1 of the present invention, the result of measuring the size of the inhibition zone (Inhibition Zone), T. rubrum And did not form lowlands for P. ovale .

Test Example 3 Test of bactericidal and antifungal efficacy against Example 3

Example 3 is a methicillin-resistant Gram-positive bacteria to measure the antimicrobial and antifungal effect of the natural fungicide composition produced by Staphylococcus aureus (Methicillin - Resistant Staphyllococcus Aureus: MRSA ) ATCC 33591, Enterococcus (E. faecalis) ATCC 29212, Bacillus subtilis (B. subtilis) ATCC 6633, L. monocytogenes jeneseu (L. monocytogenes) ATCC 19111, Staphylococcus aureus (Staphylococcus aureus) ATCC 6538, (S. mutans) and ATCC 27351, gram-negative rod bacteria of pneumonia (K. pneumoniae) ATCC 4352, pathogenic strains (P. mirabilis) ATCC 29906, Salmonella (S. typhimurium) ATCC13311, Shigella (S . flexnery) ATCC 12022, Pseudomonas aeruginosa (P. aeruginosa) ATCC 27853, Escherichia coli (E. coli O-157) ATCC 43895, Escherichia coli (Escherichia coli ) ATCC 25922, V. parahaemolyticus ATCC 33844, Yeast C. albicans ATCC 10231, Trichophyton , Athlete's Foot rubrum ) KCTC 6345, the dandruff bacillus, Pityrosporum ovale ) A test using ATCC 12078 as a test strain was performed by the Korea Institute of Chemical Fusion Testing.

1) Sterilization test method

MRSA , E. faecalis , B. subtilis , L. monocytogenes , S. aureus , S. mutans , K. pneumoniae , P. mirabilis , S. typhimurium , S. flexnery , P. aeruginosa , E. coli O-157, E . coli, V. parahaemolyticus, C. albicans to adjust so that the culture was diluted with a germ number of the initial bacterial culture 10 ⁴ ~ 10 ⁶ CFU / mL in broth and were used in the test.

Each test bacteria solution was added to 20 mL of the test solution (the natural bactericide composition obtained in Example 3), mixed, and the mixture was left at room temperature for 1 hour and 30 minutes. , MRSA was also tested after 2 hours, 8 hours, 48 hours. In addition, a test solution was prepared at a concentration of 50% (w / v) of the sample, and MRSA test bacteria were added and mixed, and the test was also performed for 1 hour 30 minutes at room temperature. Bacterial counts were measured as physiological saline, and the initial bacterial counts were expressed.

However, in the initial dilution step of the test, the test was conducted by neutralizing with D / E Neutralizing Broth (DIFCO) .In the case of growth of bacteria in the medium, the number of bacteria on the medium was multiplied by the dilution factor. When the bacteria did not proliferate, they were expressed as "less than 10 (<10)" by multiplying the dilution factor made in the neutralization step.

Bacterial counts were measured at all stages using TSA (see Section 2.2), and viable cell counts (N) were measured according to the relationship of C × N. Here, C is the colony number (the average number of colonies of two sheets), and D is the dilution factor (dilution factor of the dilution liquid).

And the sterilization reduction rate (%) was calculated from the relational expression of {(AB) / A} × 100. Here, A is the initial number of bacteria, B is the number of bacteria after a certain time.

2) Anti-fungal test method (Halo test)

① Trichophyton rubrum

Spore suspension was made from T. rubrum bacteria stored in Sabouraud Dextrose Agar and used for testing.

After incubating 200 μL of the spore suspension in the prepared plate medium (Sabouraud Dextrose Agar), treating 20 μL of the test solution to the sterilized disc (Φ 6.0.mm), and placing the disc in the center of the medium, 10 days at 25 ± 1 ° C. After incubation for the diameter of the inhibition zone (Inhibition Zone) was measured.

② Pityrosporum ovale

Pityrosporum on pre-prepared flat media Inoculated with ovale bacteria and enriched for 48 hours at 37 ℃ was used in the test. The well grown P. ovale bacteria were suspended in sterile saline solution to prepare a 10 ⁸ CFU / mL bacterial suspension, inoculated evenly on the prepared medium using a sterile swab, and 20 μL of the test solution on a sterilized disc (Φ 6.0.mm). After the treatment, the disc was placed in the center of the medium, and then cultured at 35 ± 1 ° C. for 48 hours, and then the diameter of the inhibition zone was measured. Growth inhibition zone (W) was calculated from the relationship of (TD) / 2. Where W is the width of the low zone (mm), T is the total diameter of the test solution and the low zone (mm), and D is the diameter of the test solution and disc (mm).

3) Test results

The test results are as shown in Tables 5 to 7 and FIGS. 3 to 5 below.

Sterilization Test Test strain Sample classification Early 1 hour and 30 minutes later After 2 hours After 8 hours 48 hours later

MRSA
50% (w / v) solution 8.7 × 10 ⁵ 5.4 × 10 ⁵
(37.9%)
-
-
- Undiluted solution 6.1 × 10 ⁵ <10
(> 99.9%) <10
(> 99.9%) <10
(> 99.9%) <10
(> 99.9%)

As shown in Table 5 and Figure 3, the natural disinfectant composition according to Example 3 of the present invention, when tested with the sample stock solution of the sterilization test results for MRSA initial inoculated bacterial count 1 in [6.1 × 10 ⁵ CFU / mL] After 30 minutes [<10 (less than 10) CFU / mL], after 2 hours [<1 (less than 10) CFU / mL], after 8 hours [<10 (less than 10) CFU / mL], after 48 hours [ <10 (less than 10) CFU / mL] respectively, and when tested with 50% (w / v) solution of the sample, the initial inoculated bacterial count after 1 hour and 30 minutes at [8.7 × 10 ⁵ CFU / mL] [5.4 × 10 ⁵ CFU / mL].

Sterilization Test Test strain Sample classification Early 1 hour and 30 minutes later E. coli Undiluted solution 8.9 × 10 ⁵ <10 (> 99.9%) S. aureus Undiluted solution 3.2 × 10 ⁵ <10 (> 99.9%) S. typhimurium Undiluted solution 5.2 × 10 ⁵ <10 (> 99.9%) S. flexnery Undiluted solution 3.2 × 10 ⁵ <10 (> 99.9%) P. aeruginosa Undiluted solution 5.2 × 10 ⁵ <10 (> 99.9%) E. coli  O-157 Undiluted solution 4.0 × 10 ⁵ <10 (> 99.9%) S. mutans Undiluted solution 3.1 × 10 ⁵ <10 (> 99.9%) P. mirabilis Undiluted solution 3.2 × 10 ⁵ <10 (> 99.9%) L. monocytogenes Undiluted solution 8.4 × 10 ⁵ <10 (> 99.9%) B. subtilis Undiluted solution 7.9 × 10 ⁵ <10 (> 99.9%) K. pneumoniae Undiluted solution 8.6 × 10 ⁵ <10 (> 99.9%) E. faecalis Undiluted solution 9.4 × 10 ⁵ <10 (> 99.9%) V. parahaemolyticus Undiluted solution 2.6 × 10 ⁵ <10 (> 99.9%) C. albicans Undiluted solution 1.1 × 10 ⁵ 2.0 × 10 ⁵ (81.8%)

As shown in Table 6 and Figures 3 to 5, the natural disinfectant composition according to Example 3 of the present invention is E. coli , S. aureus , S. typhimurium , S. flexnery , P. aeruginosa, E. coli O -157, S. mutans , P. mirabilis , L. monocytogenes , B. subtilis, K. pneumoniae , E. faecalis , V. parahaemolyticus , C. albicans As a result of the sterilization test for each, the initial inoculum was reduced to [<10 (less than 10) CFU / mL] after 1 hour and 30 minutes.

That is, when the bactericidal power of the natural disinfectant composition according to Example 3 of the present invention was measured for 1 hour and 30 minutes with a test solution, E. coli , S. aureus , S. typhimurium , S. flexnery , P. aeruginosa , and E. coli O-157, S. mutans , P. mirabilis , L. monocytogenes , B. subtilis, K. pneumoniae , E. faecalis , and V. parahaemolyticus all showed more than 99.9% bactericidal activity and 81.8% of C. albicans . The bactericidal power was shown.

Antifungal Test Test strain Overall diameter (T) Samples and Disc Diameter (D) Lowland width (W) Trichophyton rubrum 8.0 6.0 One Pityrosporum ovale 10 6.0 2

As shown in Table 7 and Figure 3, the natural disinfectant composition according to Example 3 of the present invention, as a result of measuring the size of the inhibition zone (Inhibition Zone), to form a low zone of 1mm against T. rubrum , P. ovale 2 mm of low zone was formed.

Test Example 4 Test of bactericidal and antifungal efficacy against Comparative Example 1

The bactericidal and antifungal effects of the natural bactericidal composition prepared by Comparative Example 1 were measured in the same manner as in Test Example 1.

The test results are shown in Tables 8 and 9 below.

Sterilization Test Test strain Early 48 hours later Sterilization Reduction Rate MRSA 6.5 × 10 ⁵ 2.2 x 10 ⁵ 66.9% or more S. mutans 2.7 × 10 ⁵ 1.1 × 10 ⁵ 68.4% or more

As shown in Table 8, the natural bactericidal composition according to Comparative Example 1, the initial inoculum bactericidal test for MRSA from [6.5 × 10 ⁵ CFU / mL] to 48 hours after [2.2 × 10 ⁵ CFU / mL] Decreased.

In addition, as a result of the sterilization test for S. mutans , the initial inoculum was reduced from [2.7 × 10 ⁵ CFU / mL] to [1.1 × 10 ⁵ CFU / mL] after 48 hours.

Antifungal Test Test strain Overall diameter (T) Samples and Disc Diameter (D) Lowland width (W) Trichophyton rubrum 6.0 6.0 0 Pityrosporum ovale 6.0 6.0 0

As shown in Table 9, the natural disinfectant composition according to Comparative Example 1, as a result of measuring the size of the inhibition zone (Inhibition Zone), T. rubrum And did not form lowlands for P. ovale .

Test Example 5 Test of bactericidal and antifungal efficacy against Comparative Example 2

The bactericidal and antifungal effects of the natural fungicide composition prepared in Comparative Example 2 were measured in the same manner as in Test Example 2.

The test results are shown in Tables 10 and 11 below.

Sterilization Test Test strain Early 48 hours later Sterilization Reduction Rate MRSA 6.5 × 10 ⁵ 2.3 × 10 ⁵ 65.7% or more S. mutans 2.7 × 10 ⁵ 1.2 × 10 ⁵ 66.3% or more

As shown in Table 10, the natural bactericidal composition according to Comparative Example 2, the initial inoculum bactericidal test results for MRSA from [6.5 × 10 ⁵ CFU / mL] to 48 hours after [2.3 × 10 ⁵ CFU / mL] Decreased.

In addition, as a result of the sterilization test for S. mutans , the initial inoculum was reduced from [2.7 × 10 ⁵ CFU / mL] to [1.2 × 10 ⁵ CFU / mL] after 48 hours.

Antifungal Test Test strain Overall diameter (T) Samples and Disc Diameter (D) Lowland width (W) Trichophyton rubrum 6.0 6.0 0 Pityrosporum ovale 6.0 6.0 0

As shown in Table 11, the natural disinfectant composition according to Comparative Example 2, as a result of measuring the size of the inhibition zone (Inhibition Zone), T. rubrum And did not form lowlands for P. ovale .

Therefore, the natural disinfectant composition according to the embodiment of the present invention can effectively prevent food poisoning by maintaining hygienic and clean state when used as a disinfectant while having excellent antiseptic power and antifungal effect on bacteria and fungi when used on human skin. .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments or constructions. Various changes and modifications may be made without departing from the spirit and scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

The natural disinfectant composition according to the present invention may be applied to beverages, feed, food, medicine, cosmetics, etc. to suppress the growth of harmful pathogens and microorganisms and to remove odors caused by microorganisms with the use of sterilizing Gram-positive and Gram-negative bacteria. In addition, it can be used for various purposes, such as medical fungicides for treating various skin diseases such as athlete's foot and agricultural fungicides used to control fungal diseases and fungal diseases that cause severe damage to plants.

In addition, synthetic pesticides can be used as insect repellents, which can be expected to improve the quality and yield of organic products, as well as to improve agricultural productivity and agricultural competitiveness.

Claims (8)

Natural fungicide composition containing ginseng extract, garlic extract, encyclopedia extract, Schisandra chinensis extract and Sancho extract as active ingredients.
The method of claim 1,
95-100 parts by weight of the ginseng extract, 95-100 parts by weight of the garlic extract, 190-200 parts by weight of the encyclopedia extract, 48-50 parts by weight of the Schizandra chinensis extract and 48-50 parts by weight of the acidic extract Natural disinfectant composition, each containing in proportion.
The method of claim 1,
Mugwort extract, pogongyoung extract, ginger extract, nacho extract, cornus extract, plum extract, pomegranate extract, amorous extract and natural extract further comprises at least one selected from the group consisting of extract.
The method of claim 3, wherein
Natural disinfectant composition containing the mugwort extract, the pogongyoung extract, the ginger extract, the reef extract, the cornus extract, the plum extract, the pomegranate extract, the azu extract and the creation extract in a composition ratio of 48 to 50 parts by weight, respectively .
5. The method according to any one of claims 1 to 4,
Each of the extract is a natural disinfectant composition consisting of a distillate to extract the decoction.
A method for producing a natural disinfectant composition comprising the following steps.
(A) 95-100 parts by weight of ginseng, 95-100 parts by weight of garlic, 190-200 parts by weight of encyclopedia, 48-50 parts by weight of Schizandra chinensis, and 48-50 parts by weight of acidic fermentation at 34-36 ° C. for 70-72 hours
(B) a step of drying each component passed through the step (A) at 50 ~ 60 ℃ for 46 to 50 hours
(C) a step of mixing each component passed through the step (B) with 1000 parts by weight of ethyl alcohol and aging for 22 to 24 hours at 75 ~ 80 ℃
(D) process of the mixture passed through the step (C) at 100 ℃ for 120-150 minutes
(E) extracting the distillate while further sweetening the mixture passed through step (D) at 100 ° C. for 7-8 hours.
A method for producing a natural disinfectant composition comprising the following steps.
(A) 95-100 parts by weight of ginseng, 95-100 parts by weight of garlic, 190-200 parts by weight of encyclopedia, 48-50 parts by weight of Schizandra chinensis, and 48-50 parts by weight of acidic fermentation at 34-36 ° C. for 70-72 hours
(B) a step of drying each component passed through the step (A) at 50 ~ 60 ℃ for 46 to 50 hours
(C) a step of mixing each component passed through the step (B) with 1000 parts by weight of ethyl alcohol and aging for 22 to 24 hours at 75 ~ 80 ℃
(D) process of the mixture passed through the step (C) at 100 ℃ for 120-150 minutes
(E) extracting the decoction solution by filtration and concentrated under reduced pressure at 30 ~ 60 ℃ the mixture obtained through the step (D)
The method according to claim 6 or 7,
48 to 50 parts by weight of mugwort in the step (A), 48 to 50 parts by weight of pogongyoung, 48 to 50 parts by weight of ginger, 48 to 50 parts by weight of corn, 48 to 50 parts by weight of cornus oil, 48 to 50 parts by weight of plum and pomegranate 48 Method for producing a natural disinfectant composition further containing at least any one selected from ~ 50 parts by weight.

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