KR20130047415A - Fragrance composition comprising fermented extract of mushroom mycelial - Google Patents

Abstract

The present invention relates to a fragrance composition comprising an extract extracted from the fermentation broth of at least one mushroom mycelium selected from basidiomycetes or asymptococci as an active ingredient.
Extract extracted from the fermentation broth of basidiomycete or asymptococcus mushroom mycelium according to the present invention is safe as a natural extract, does not have toxicity and side effects, and does not irritate skin. In addition, since the strength and preference of the fragrance is high, and the fragrance has a long lasting effect, it can be usefully used as a fragrance composition, cosmetic composition, fragrance composition and quasi-drug composition.

Description

Fragrance composition comprising fermented extract of mushroom mycelial

The present invention relates to a fragrance composition comprising a extract extracted from the fermentation broth of at least one mushroom mycelium selected from basidiomycetes or asymptococci as an active ingredient and a method for preparing the same.

Essential oils, which are the main ingredients of the fragrance composition, are volatile oils mainly composed of monoterpenes, hydrocarbons of cisquiterpenes and derivatives thereof, and distill, compress, extract or cool the flowers, leaves, stems and roots of fragrant plants. It can be obtained by the method, and there are hundreds or thousands of kinds of essential oils.

Many products such as food, medicine, daily necessities and cosmetics widely used in our surroundings have been added with various fragrance-flavoring compositions. In particular, mushroom flavor is known to restore the mind and body fatigue in oriental medicine, strengthen the kidneys to give a bright and pleasant mood, regardless of age and sex, has been widely used as a fragrance composition.

On the other hand, the material used as a fragrance composition, although there are some substances produced in nature, most of the fragrance composition is a chemical synthetic synthetic materials. Such chemically synthesized fragrance composition has a problem that the fragrance components cause allergies, the fragrance composition produced in nature has a problem that there is a limit in the strength and persistence of the fragrance.

Thus, the present inventors have completed the present invention by confirming that the fragrance and fragrance persistence is enhanced through the mixing of the mushroom mycelium fermentation, the strength of the flavor is increased.

In the present invention, it is possible to provide a fragrance composition comprising a extract extracted from the fermentation broth of at least one mushroom mycelium selected from basidiomycetes or asymptomatic fungi as an active ingredient and a method for producing the same.

In addition, the present invention can provide a cosmetic composition, fragrance composition and quasi-drug composition comprising the perfume composition.

The present invention relates to a fragrance composition comprising an extract extracted from the fermentation broth of at least one mushroom mycelium selected from basidiomycetes or asymptococci as an active ingredient.

Hereinafter, the perfume composition according to the present invention will be described in more detail.

Mushrooms are usually composed of fruiting bodies provided for food or medicinal purposes and mycelium equivalent to the root or root of mushrooms. The fruiting body functions as a propagation organ for mushrooms, and the mycelium functions as a nutrient organ for supplying nutrients to the fruiting body.

Mushrooms have been used for edible or medicinal mushrooms, but biliary fungi such as shiitake mushrooms contain more nutrients and active ingredients in mycelia than fruit bodies.

The fragrance composition according to the present invention comprises an extract extracted from the fermentation broth of one or more mushroom mycelium selected from basidiomycetes or asymptococci (hereinafter, basidiomycetes or aseptic fungus mycelium fermentation extract) as an active ingredient.

At this time, as the basidiomycete, mushrooms selected from the group consisting of creased fungi, Democratic fungi, Bacillus spruce, Throat bark and Moss bark may be used.

Examples of the pleated mushrooms include net mushrooms including king net mushrooms, mud mushrooms including mud mushrooms and chopped mushrooms, giant mushrooms including giant mushrooms, and cherry mushrooms including cherry mushrooms. Pleurotus eryngii, including Pleurotus eryngii, Pleurotus eryngii, Pleurotus eryngii, Pleurotus eryngii, Shiitake, Pleurotus eryngii, Pleurotus eryngii, Pine mushroom, Pine mushroom, Mulberry, Pleurotus eryngii, Mulberry mushroom, Wheat Pine mushrooms including mushrooms, moss mushrooms and oyster mushrooms, or horned mushrooms and mushrooms including horned mushrooms, etc. can be used. As democratic mushrooms, oriole mushrooms including oriole, etc. Trumpet mushrooms, such as morning glory mushrooms, noodle mushrooms, etc. Bearded mushrooms including beard mushrooms, Pear blossoms including white pear mushrooms and morning glory pear mushrooms, Flower cloud mushrooms including flower cloud mushrooms, Flower mushrooms including zinnia mushrooms Worm mushrooms, including wormwood, leafy mushrooms, clamshell mushrooms, zombie mushrooms, horseshoe butterfly mushrooms and flower pot mushrooms, spear mushrooms and cactus mushrooms Pine scales and mushrooms, etc. may be used, and the fungus mushrooms include horseshoe mushrooms including horseshoe mushrooms and horseshoe mushrooms, fart mushrooms including scarlet fart mushrooms, and bird nest mushrooms. Mug mushrooms, steamed bread mushrooms, including white steamed bread mushrooms, dust mushrooms including dust mushrooms, pile mushrooms including pile mushrooms and yellow manure mushrooms, or red basket mushrooms and three Basket mushrooms and mushrooms, including paw mushrooms, can be used. In addition, as a tree thirsty mushroom, can be used as a tree, including a soybean and a hairy mushroom, or a tree and a fungus including a zombie mushroom and sessile mushroom, and as a cedar mushroom, a nettle mushroom, a white tree Cedar mushrooms including mushrooms and Cushion cedar mushrooms can be used.

In addition, in the present invention, as the aseptic fungus, mushrooms selected from the group consisting of main mushrooms and rubber mushrooms may be used.

The main mushrooms include the hairy rubber mushrooms including brown hairy rubber mushrooms, the hairy mushrooms including hairy mushrooms, the plate mushrooms including plate mushrooms, the morel mushrooms including morel, Saddle mushrooms and mushrooms, including saddle mushrooms and little saddle mushrooms, may be used. Gum mushrooms include hood mushrooms, including rubber mushrooms, tuber mushrooms, soybean mushrooms, yellow rubber mushrooms, and turmeric rubber mushrooms. Alternatively, bean sprouts mushrooms and mushrooms, including pitching mushrooms, may be used.

In the present invention, preferably basidiomycete mushroom may be used, and more preferably, shiitake mushroom and situation mushroom may be used together.

Shiitake mushrooms are native to broad-leaved trees, specifically, chestnut, prunus oak or oak, and are effective for the prevention of hypertension and arteriosclerosis because they lower cholesterol and lower blood pressure. Known. Aromatic components of the shiitake mushroom are represented by the decomposition of rethionine.

Situation mushroom is a perennial mushroom of basidiomycete which grows in the old tree of mountain mulberry inhabiting alpine region, and is known to have excellent effects such as anticancer effect, human immunity enhancement and detoxification effect. Due to the efficacy of such situation mushrooms, situation mushrooms may be added as part of the herbal composition or used as an independent medicine in the manufacture of therapeutic or botanical herbal medicines, or may be used to soak alcohol.

Situation mushrooms are also called woody mud mushrooms. In Dongbobogam, it is a herb that is recorded on the liquid juice in the name of Sangmokyi (桑 木耳). Situation of mushrooms is 6-12 cm in diameter and 2-10 cm in thickness, and has various shapes such as semicircle, flat, round mountain shape or horseshoe shape, and the surface grows short and dense with dark brown hairs. It will disappear and become cuticles. In addition, the adult has a blackish brown ring groove and the horizontal and vertical backs are separated. The edges are bright yellow, the lower side is tan, and the flesh is yellowish brown. No sack, spores are light yellowish brown, ball-shaped.

More specifically, the situation mushroom is a perennial plant native to Korea, Japan, Australia, and North America. It is a woody fungus that overlaps with mulberry trees. Also called (樹 舌).

Fermented extract of Basidiomycetes or Aspergillus fungus Mycelium in the fragrance composition according to the present invention is inoculated with Basidiomycetes or Aspergillus mycelium;

Fermenting the inoculated mycelium; And

The fermented mycelium may be prepared by extracting with a solvent.

First, in the step of inoculating mycelium, the above-described types of basidiomycetes and streptococci can be used without limitation, preferably shiitake mushrooms and situation mushrooms can be used.

Inoculation of Basidiomycetes or Aspergillus mycelium in the present invention may be used to cut the folds of the folds of basidiomycetes and Aspergillus fungus in a clean bench, and then transplanted into PDA medium. Three to four weeks after transplantation of the pleural site fragments are transferred to fresh PDA medium and cultured. The culture cultured by the above process can be used as a progenitor of liquid culture. The prokaryotes may be used by incubating for 2-3 weeks in a 26 ° C. incubator using a PDA solid medium.

Fermentation culture of the mycelia in the present invention may include the step of culturing the mycelium in ATM-DY medium (liquid culture) and the step of fermentation for 1 to 2 weeks at 20 to 35 ℃.

Liquid culture in the present invention is used by cutting the mycelium cultured in the above-described PDA medium to the size of 4 X 4 mm, ATM-DY medium (dextrin: 25g / L, yeast extract: 8g / L, KH 2 PO 4: 2g / L, MgSO47H20: 1 g / L) can be used as the primary liquid culture. The primary cultured inoculum, ie, mycelium, can be cultured in an optimal medium suitable for mycelial growth environment.

The main culture can adjust the fermentation conditions in consideration of the type of fermented strains inoculated, for example 20 to 35 ℃, can be fermented for 1 week to 2 weeks, preferably 26 ℃ and can be fermented for 3 weeks have.

In the present invention, after the fermentation is completed, sterilization treatment may be further performed to kill basidiomycetes or aseptic fungi.

Mycelium fermented in the present invention can be prepared as a mycelium fermentation extract through solvent extraction.

In the extraction, an inorganic solvent or an organic solvent may be used as the solvent.

In the case of using an inorganic solvent in the extraction, water is added to the fermented mycelium, heated at 70 ° C or higher, preferably 80 ° C or higher, more preferably 90 ° C or higher, for 2 hours or longer, preferably 3 hours or longer, and heated. The prepared mixture can be extracted by filtration. At this time, when heated at a temperature higher than 90 ℃, the heating time is reduced, but there is a fear that the active ingredient is destroyed and lost, when heated at a temperature lower than 70 ℃, there is a fear that the active ingredient of the mixture is not extracted completely.

In addition, when using an organic solvent in the extraction, any extraction organic solvent commonly used in the art can be used, preferably alcohols such as methanol, ethanol, isopropanol and butanol, acetone, hexane, chloroform, ethyl acetate , Butylene glycol or a mixture thereof can be used, and preferably 1,3-butylene glycol can be extracted at room temperature or by heating.

The amount of the solvent in the present invention varies depending on the type of solvent used, preferably can be used in an amount of 10 to 40% by weight of the total weight of the fermentation product.

In the present invention, in order to remove suspended solid particles in the extracted extract, that is, mycelium fermentation extract, it can be filtered using a filter such as nylon or using a freeze filtration method.

Basidiomycete or Aspergillus mycelium mycelium fermentation extract of the present invention includes not only the extract by the aforementioned extraction solvent, but also an extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the basidiomycete or asymptococcus mycelium fermentation extract of the present invention.

In addition, the mycelium fermentation extract of the present invention may be prepared in a powder state by an additional process such as vacuum distillation and freeze drying or spray drying.

The present invention also relates to a cosmetic composition comprising the perfume composition described above.

In the cosmetic composition of the present invention, the perfume composition may be included in an amount of 0.0001 to 30 parts by weight, preferably 1 to 30 parts by weight, and more preferably 1 to 10 parts by weight, based on the total weight of the cosmetic composition. If the content of the fragrance composition is less than 0.0001 parts by weight, the fragrance is very low, it may be difficult to taste the fragrance in the manufactured cosmetics, if it exceeds 30 parts by weight, the fragrance is too strong for use as a cosmetic.

In the present invention, the cosmetic composition may include components conventionally used in addition to the above-described perfume composition (microbial fungus or mycelium fermentation extract) as an active ingredient. Such commonly used ingredients include, for example, adjuvants or carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. In addition, the cosmetic composition may further include a skin absorption promoting substance.

Cosmetic compositions in the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing It can be formulated as oil, powder foundation, emulsion foundation, wax foundation and spray. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

In the present invention, when the formulation of the cosmetic composition is a paste, cream or gel, the carrier component may be animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide. Can be used.

In addition, when the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as the carrier component, especially in the case of a spray, chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

In addition, when the formulation of the cosmetic composition is a solution or an emulsion, a solvent, a solubilizer or an emulsifier may be used as the carrier component, specifically, water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate , Propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan and the like can be used.

In addition, when the formulation of the cosmetic composition is a suspension, a liquid diluent such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

In addition, when the formulation of the cosmetic composition is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid. Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The present invention also relates to a perfume composition comprising the perfume composition described above.

In the perfume composition of the present invention, the perfume composition may be included in an amount of 0.0001 to 30 parts by weight, preferably 1 to 30 parts by weight, and more preferably 1 to 10 parts by weight, based on the total weight of the perfume composition. If the content of the fragrance composition is less than 0.0001 parts by weight, the fragrance is very low, it is difficult to taste the fragrance in the manufactured fragrance, if it exceeds 30 parts by weight, the fragrance is too strong for use as a fragrance.

In the present invention, when the perfume composition is used as an additive of the perfume composition, the perfume composition may be used as it is, or may be used in admixture with any component useful for the flavor or perfume composition.

In particular, the fragrance composition can be used in admixture with one or more of a wide variety of natural, synthetic, synthetic chemicals, natural fragrances, flavoring substances, flavors or natural extracts used in the field of fragrances.

In addition, the fragrance composition may contain one or more ingredients or excipients commonly used with flavoring and fragrances, such as carrier materials, thickening agents, flavor enhancers and other auxiliaries commonly known and used in the art. Can be.

The fragrance composition in the present invention may provide a gel or solidified composition for use in a volatile fragrance, or may provide a liquid composition for use in a spray fragrance.

Moreover, this invention relates to the quasi-medicine composition containing the fragrance composition mentioned above. That is, the fragrance composition of the present invention may be added to the quasi-drug composition for the purpose of smelling the mixed aroma of the fermented extract of mushroom mushroom mycelium mycelium and shiitake mushroom mycelium, for example.

When the perfume composition is used as an additive of the quasi-drug composition in the present invention, it can be suitably used according to conventional methods such as using the fragrance composition as it is, or using together with other quasi-drugs or quasi-drug components.

The content of the fragrance composition in the present invention may be appropriately determined according to the purpose of use of the quasi-drug, for example, 0.0001 to 30 parts by weight, preferably 1 to 30 parts by weight and more preferably 1 to the total weight of the quasi-drug composition. It may be included in an amount of 10 parts by weight. If the fragrance composition is less than 0.0001 parts by weight, the fragrance is very low, it is difficult to savor the aroma of the quasi-drug composition to be prepared, if it exceeds 30 parts by weight becomes too strong to be used as a quasi-drug.

In the present invention, the quasi-drug composition is a product selected from the group consisting of antiseptic cleaner, shower foam, detergent, dishwashing detergent, toothpaste, mouthwash, wet tissue, detergent soap, hand wash, shampoo, humidifier cleaner, mask, ointment and filter cleaner. Can be used as

Basidiomycete or Aspergillus mycelium fermentation extract according to the present invention is safe as a natural extract, has no toxicity and side effects, and does not irritate the skin. In addition, since the strength and preference of the fragrance is high, and the fragrance has a long lasting effect, it can be usefully used as a fragrance composition, cosmetic composition, fragrance composition and quasi-drug composition.

Hereinafter, preferred embodiments and experimental examples are provided to facilitate understanding of the present invention. However, the following examples and experimental examples are provided only to more easily understand the understanding of the present invention, and the contents of the present invention are not limited by the examples.

Example  1. Preparation of Shiitake Mycelium and Situary Mushroom Mycelium Fermented Extract

In the fruiting bodies of natural shiitake mushrooms and situation mushrooms, fresh wrinkled sections were cut under sterile conditions and transplanted into PDA medium. After 3-4 weeks, the cells were transferred to fresh PDA medium, cultured, and used as progenitors of liquid culture. Probiotics were used by incubating for 2-3 weeks in an incubator at 26 ℃ using a PDA solid medium.

Liquid culture in the present invention is a mycelium (progenus) cultured in PDA medium cut to 4 × 4 mm size and ATM-DY medium (dextrin: 25g / L, yeast extract: 8g / L, KH ₂ PO ₄ : 2g / L, MgSO ₄ 7H ₂ 0: 1 g / L) was used as the primary liquid culture. The primary inoculum was cultured in an optimal medium suitable for the mycelial growth environment.

After fermentation at 26 ° C. for 3 weeks, fermentation was confirmed by varying turbidity of pH and mycelial growth. After the fermentation is completed, sterilize by heating at 121 ℃ for 15 minutes to kill the inoculated fermentation bacteria, 1,3-butylene glycol (1,3 Butylene Glycol) to 30% by weight of the total volume of the fermented mushrooms The mycelium fermented extract was extracted to 70% by weight. After cooling the extract for 3 days, soybean fungus mycelium fermented extract was prepared by filtration with a 0.2 μm filter so that no precipitate formed.

Example  2. Preparation of Cream Containing Shiitake Mushroom Mycelium and Situation Mushroom Mycelium Fermented Extract

Fermented Basidiomycete Mycelium and Fermented Basidiomycete Mycelium Fermentation in order to evaluate the aroma when applying the basidiomycete mycelium fermentation extract (mixture of shiitake mycelium fermentation extract and situation mushroom mycelium fermentation extract) prepared in Example 1 Creams each containing an extract were prepared.

Table 1 shows the extracts of basidiomycetes uncultured basidiomycete extract, basidiomycetes culture extract extracts fermented by mycelial culture.

Raw material name Basidiomycetes extract (parts by weight) Basidiomycetes culture extract (parts by weight) Set Steyl Alcohol 3.0 3.0 Self-emulsifying monostearic acid 1.5 1.5 Lipophilic monostearic acid 1.5 1.5 Wax 0.5 0.5 Liquid paraffin 6.0 6.0 Squalane 8.0 8.0 Sorbitan monosulfate 1.0 1.0 glycerin 8.0 8.0 Propylene glycol 4.0 4.0 Betaine 4.0 4.0 Basidiomycetes Extract Suitable amount - Basidiomycetes culture extract - Suitable amount Pigment Suitable amount Suitable amount Preservative Suitable amount Suitable amount Purified water To 100 To 100

Example  3. Preparation of Softened Longevity Containing Shiitake Mushroom Mycelium and Situary Mushroom Mycelium Fermented Extract

A flexible lotion including a basidiomycete mycelium fermentation extract (a mixture of shiitake mycelium fermentation extract and a situation mushroom mycelium fermentation extract) prepared in Example 1 was prepared.

Table 2 shows the extracts of basidiomycetes, which were not cultured basidiomycetes extract, and basidiomycetes culture extracts represent the extracts fermented by mycelial culture.

Raw material name Basidiomycetes extract (parts by weight) Basidiomycetes culture extract (parts by weight) ethanol 10 10 Polyoxyethylene sorbitan polylauric acid One One glycerin 5 5 1,3-butylene glycol 3 3 Sodium benzoate Suitable amount Suitable amount Mushroom Extract Suitable amount - Mushroom Culture Extract - Suitable amount Purified water To 100 To 100

Example  4. Preparation of essence comprising shiitake mushroom mycelium and situation mushroom mycelium fermented extract

An essence was prepared comprising the basidiomycete mycelium fermentation extract (mixture of shiitake mycelium fermentation extract and shiitake mushroom mycelium fermentation extract) prepared in Example 1.

Table 3 shows the extracts of basidiomycetes uncultured, and basidiomycetes extract extracts fermented by mycelial culture.

Raw material name Basidiomycetes extract (parts by weight) Basidiomycetes culture extract (parts by weight) Propylene glycol 10 10 Sodium hyaluronate aqueous solution (1%) 5 5 glycerin 5 5 1,3-butylene glycol 3 3 Polyoxyethylene Cured Castor Oil One One Sodium benzoate Suitable amount Suitable amount Mushroom Extract Suitable amount - Mushroom Culture Extract - Suitable amount Purified water To 100 To 100

Example 5 Preparation of an external dermal ointment comprising shiitake mushroom mycelium fermentation extract

An external skin ointment was prepared, including the basidiomycete mycelium fermented extract prepared in Example 1 (a mixture of shiitake mycelium fermented extract and a situation mushroom mycelium fermented extract).

Basidiomycete extract of Table 4 represents the uncultured basidiomycetes extract, basidiomycete culture extract represents an extract fermented by mycelial culture.

Raw material name Basidiomycetes extract (parts by weight) Basidiomycetes culture extract (parts by weight) Diethyl sebacate 8 8 Prepayment 5 5 Polyoxyethylene oleyl ether phosphate 6 6 Sodium benzoate Suitable amount Suitable amount Mushroom Extract Suitable amount - Mushroom Culture Extract - Suitable amount vaseline To 100 To 100

Example 6 Preparation of Fragrances Comprising Shiitake Mushroom Mycelium and Situary Mushroom Mycelium Fermented Extract

A fragrance was prepared comprising the basidiomycete mycelium fermentation extract prepared in Example 1 (a mixture of shiitake mushroom mycelium fermentation extract and situation mushroom mycelium fermentation extract).

The basidiomycete extract of Table 5 represents the uncultivated basidiomycetes extract, and the basidiomycetes culture extract represents an extract fermented by mycelial culture.

Raw material name Basidiomycetes extract (parts by weight) Basidiomycetes culture extract (parts by weight) Polyoxyethylene alkyl ether 6 6 Sodium cumene sulfonate 0.5 0.5 Cetyl ether 10 10 Caster oil 5 5 ethanol 5 5 gelatin 3 3 Sodium benzoate Suitable amount Suitable amount Mushroom Extract Suitable amount - Mushroom Culture Extract - Suitable amount Purified water To 100 To 100

Experimental Example 1. Evaluation of fragrance of basidiomycete mycelium fermented extract

In order to evaluate the fragrance of the mycelial fermented extract of Basidiomycetes, a panel evaluation was conducted on the intensity and preference of 10 adult men and women. The specific evaluation was conducted by blind test, and prepared shiitake mushroom sample, shiitake mushroom sample, shiitake mushroom mycelium sample, situation mushroom mycelium sample and mycelium sample thoroughly fermented for 3 weeks, and the panels were infused one by one, Evaluation was made. The higher the number, the stronger the scent and the greater the preference. In order to increase the objectivity of the fragrance evaluation, one smell was taken and then the next smell was taken at intervals of 10 minutes. The average value of the panel evaluation is shown in Table 6 below.

Incense Strength: 1 none, 2 weak, 3 medium, 4 strong, 5 very strong

Fragrance preference: 1 ~ 5 degree of preference, the larger the number

The numerical value of each item represents the average value of panel evaluation.

burglar preference Shiitake mushrooms 1.3 2.5 Situation Mushroom 1.4 2.2 Shiitake mycelium 2.1 2.8 Situation Mushroom Mycelium 2.4 2.4 Shiitake mushroom mycelium + Situation mushroom mycelium (fermented sample) 3.5 3.3

As shown in Table 6, fermentation and mixing of each mycelium obtained a higher value in the intensity of the fragrance than the individual mycelium, it can be seen that the preference is also increased.

Experimental Example 2. Stability test of basidiomycete mycelium fermented extract.

Distilled water was added to the basidiomycete mycelium fermentation extract prepared in Example 1 to prepare a 5% aqueous solution, and then light stability and thermal stability were tested.

Light stability was continuously exposed to sunlight for 4 and 8 hours using a light resistance tester (Suntest CPS +). The light stability test results are shown in Table 7 below.

Sun exposure time (hr) Test results (compared to the original state and discoloration, the smell) 4 hr Good 8 hr Good

As shown in Table 7, the basidiomycete mycelium fermentation extract can be confirmed that there is no discoloration and discoloration even after exposure to sunlight continuously for 8 hours.

Thermal stability was compared for discoloration and odor by heating continuously for 4 hours and 8 hours using an 80 ℃ thermostat. The thermal safety test results are shown in Table 8 below.

Heating time (hr) Test results (compared to the original state and discoloration, the smell) 4 hr Good 8 hr Good

As shown in Table 8, the basidiomycete mycelium fermentation extract can be confirmed that there is no discoloration and odor even after heating continuously for 8 hours.

Therefore, it can be seen that the perfume composition of the present invention is stable from sunlight and heat.

Experimental Example 3. Skin Patch Test

In order to test the skin irritation of the cream prepared in Example 2, a patch test was performed using guinea pigs according to the method of Draize (Draize, JH ,: Dermal toxicity.Assoc. Food and Drug officials, US.Appraisal of the safety of chemicals in Food, Drugs and Cosmetics.pp46-59, Texas State Dept. of Health, Austin, Texas, 1959; Federal Register, Method of testing primary irritant substances 38 (187), pp1500 -1541, 1973).

First, the hair of the guinea pig and the like was removed for sample application, and then the environment was adapted for 24 hours to minimize skin irritation. After acclimation to the environment for 24 hours, the sample application site was set to an area of 1.5 cm X 1.5 cm, and the gauze was applied after applying the cream prepared in Example 2 above. Thereafter, to prevent evaporation and loss of the cream was sealed with a solid foil and fixed with an elastic bandage for 48 hours.

After removing the closed patch, the degree of stimulation was determined at 2 hours and 24 hours, and the stimulation index (PII) and the degree of stimulation are shown in Table 9 below.

sample Stimulus Index (PII) Irritation degree Contains fungal extract 0.71 stimulus Contains basidiomycetes culture extract 0.39 Microstimulation

As shown in Table 9, it can be seen that the cream containing basidiomycete culture extract is only half the level of stimulation of the cream containing basidiomycete extract.

Therefore, the fragrance composition of the present invention can be usefully added to cosmetic compositions, perfume compositions, quasi-drug compositions, and the like.

Experimental Example 4. Evaluation of Persistence of Basidiomycetes Fermented Extract

Persistence of fragrance was evaluated for the cream formulation prepared in Example 2. The evaluation results are shown in Table 10.

1 month stable: lasts 1 month without odor

1 month of odor: changes in scent within 1 month

Cycle: Temperature change condition of -10 ℃ (6 hours) → 25 ℃ (6 hours) → 45 ℃ (6 hours) → 25 ℃ (6 hours) based on 24 hours

Freezing: Repeated sample freezing and thawing

Condition Contains basidiomycetes extract Contains basidiomycetes culture extract 0 ℃ 1 month stable 1 month stable 40 ℃ 1 month stable 1 month stable 45 ° C 1 month 1 month stable Cycle 1 month 1 month stable Frozen (-15 ℃ <-> room temperature) 2 times bad smell 3 times stable

As shown in Table 10, it can be seen that the basidiomycete culture extract is longer than the basidiomycetes extract, the fragrance lasts longer without anomalous, especially the persistence of the fragrance in the situation of temperature change is superior to the basidiomycetes extract have.

Claims (11)

Perfume composition comprising an extract extracted from the fermentation broth of at least one mushroom mycelium selected from basidiomycetes or aseptic fungi as an active ingredient.
The method of claim 1,
Basidiomycete is a mushroom composition selected from the group consisting of fungi, Democratic mushrooms, Bacillus spp.
The method of claim 1,
Aspenic fungus is a mushroom composition selected from the group consisting of main mushrooms and mushrooms.
Cosmetic composition comprising the perfume composition according to claim 1 as an active ingredient.
The method of claim 4, wherein
Cosmetic composition comprising 0.0001 to 30 parts by weight relative to the total weight of the cosmetic composition.
The method of claim 4, wherein
The cosmetic composition is one or more formulations selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays Cosmetic composition.
Perfume composition comprising the perfume composition according to claim 1 as an active ingredient.
The method of claim 7, wherein
Perfume composition comprising a 0.0001 to 30 parts by weight relative to the total weight of the perfume composition.
A quasi-drug composition comprising the perfume composition according to claim 1 as an active ingredient.
The method of claim 9,
The quasi-drug composition comprising the perfume composition 0.0001 to 30 parts by weight based on the total weight of the quasi-drug composition.
The method of claim 9,
The quasi-drug composition is selected from the group consisting of disinfectant cleaners, shower foams, detergents, dishwashing detergents, toothpastes, mouthwashes, wet wipes, detergent soaps, hand washes, shampoos, humidifier cleaners, masks, ointments and filter cleaners.