KR102113645B1 - A cosmetic composition for preventing hair loss and promoting hair growth containing spicule powder and natural extract - Google Patents

Abstract

The present invention provides a cosmetic composition comprising a fine needle powder and a natural extract as an active ingredient.

Description

A cosmetic composition for preventing hair loss and promoting hair growth, including fine needle powder and natural extracts as an active ingredient {A COSMETIC COMPOSITION FOR PREVENTING HAIR LOSS AND PROMOTING HAIR GROWTH CONTAINING SPICULE POWDER AND NATURAL EXTRACT}

The present invention relates to a cosmetic composition comprising a fine needle powder and a natural extract as an active ingredient.

Animals have a molting period in which hairs are replaced entirely depending on the season, but in humans, a similar number is maintained as some of the hairs are continuously replaced every day.

The hair cycle can be divided into three cycles: growth phase, degeneration phase, and resting phase. Among them, the anagen phase is a period in which active cell division occurs in the papilla, and the growth of new hair is promoted. Can be.

The growth cycle of hair is approximately 3 to 5 years for men and 4 to 6 years for women, and approximately 80 to 85% of hair is in the growth phase.

The catagen is a period in which cell division gradually stops and is about 3 to 4 weeks.

Lastly, the telogen is a period when the hair papillae is atrophied and the hair is separated from the capillaries and is simply embedded in the scalp. It is about 3 months.

Although human hair is irrelevant to life, a lot of attention has been focused on socially because it has a decisive effect on a person's impression and appearance.

In particular, as the recent advancement and acceleration of industrialization, the hair loss population is rapidly increasing due to excessive stress and social and cultural factors of lifestyle. In addition, recently, not only middle-aged men but also young people are increasing hair loss, and research into analyzing various causes of hair loss and solving them has been actively conducted.

Meanwhile, in the cosmetics industry, many products using natural products have been developed to reduce skin irritation caused by various chemicals. Natural ingredients have fewer side effects on skin, and recently, as consumers' response to cosmetics using natural ingredients has increased, the development value of cosmetics has increased.

Therefore, the demand for cosmetics using natural materials with little side effects and high consumer response is gradually increasing, and various studies on multi-funtional cosmetics development with excellent biosafety and skin improvement effect are actively conducted. have.

The present invention is to solve the problems of the prior art described above, the object of the present invention is to provide a functional cosmetic composition that has less irritation to the skin, prevents hair loss and effectively promotes hair growth.

According to one aspect of the invention, the fine needle powder; And it is provided with a cosmetic composition comprising as an active ingredient; one or more extracts selected from the group consisting of medicinal wheat extract, plantain extract, Hannyeoncho extract and fresh herb extract.

In one embodiment, the extract may be a fermentation extract prepared by inoculating a fermentation strain.

In one embodiment, the fermentation strain may be one or more mycelium selected from the group consisting of Pleurotusus ferulae , Ganoderma lucidum , Hericonium erinaceum and Phellinus linteus .

In one embodiment, the cosmetic composition may further include a coffee silver skin extract.

In one embodiment, the cosmetic composition may further include a first complex extract composed of a medicinal extract, an extract of medicinal moss, a mugwort extract, an Angelica extract, a black bean extract, and a peony extract.

In one embodiment, the cosmetic composition may further include a second complex extract consisting of ginkgo biloba extract, sewage extract, centella extract, green tea extract and aloe vera extract.

In one embodiment, the cosmetic composition may be for preventing hair loss and promoting hair growth.

In one embodiment, the cosmetic composition may be for suppressing inflammation of the scalp.

In one embodiment, the cosmetic composition is a hair shampoo, hair conditioner, hair conditioner, hair treatment, hair tonic, scalp treatment, hair lotion, hair cream, hair mist, hair spray, hair ampoule, hair pack, hair essence , Hair oil, scalp scale, hair nutrient longevity and general ointment may be formulated with one or more selected from the group consisting of.

The cosmetic composition according to an aspect of the present invention may contain a fine needle powder and a natural extract as an active ingredient to prevent and treat hair loss while minimizing skin irritation and realize an effect of promoting hair growth.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the invention.

The terms used in the present specification have selected general terms that are currently widely used as possible while considering functions in the present invention, but this may be changed according to the intention or precedent of a person skilled in the art or the appearance of new technologies.

In addition, in certain cases, some terms are arbitrarily selected by the applicant, and in this case, their meanings will be described in detail in the description of the applicable invention. Therefore, the terms used in the present invention should be defined based on the meanings of the terms and the contents of the present invention, not simply the names of the terms.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person skilled in the art to which the present invention pertains. Terms such as those defined in a commonly used dictionary should be interpreted as having meanings consistent with meanings in the context of related technologies, and should not be interpreted as ideal or excessively formal meanings unless explicitly defined in the present application. Does not.

Numeric ranges are inclusive of the numerical values defined in the above range. All maximum numerical limits given throughout this specification include all lower numerical limits as if the lower numerical limits were clearly written. All minimum numerical limits given throughout this specification include all higher numerical limits as higher numerical limits are clearly written. All numerical limits given throughout this specification will include all better numerical ranges within a broader numerical range, as narrower numerical limits are clearly written.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited by the following examples.

According to one aspect of the invention, the fine needle powder; And it is provided with a cosmetic composition comprising as an active ingredient; one or more extracts selected from the group consisting of medicinal wheat extract, plantain extract, Hannyeoncho extract and fresh herb extract.

The cosmetic composition includes an extract derived from nature as an active ingredient to prevent hair loss, promote hair growth, and have excellent biosafety, as well as minimize skin irritation.

The "comprising as an active ingredient" may exhibit a hair loss prevention and hair growth promoting effect, for example, an activity inhibitory effect on 5 alpha-reductase, which is known as a major cause of hair loss, and can prevent hair loss due to scalp inflammation. It may mean that it contains an effective amount of a degree capable of exhibiting an activity inhibitory effect or the like of prostaglandin E2 (PGE2).

In particular, since the cosmetic composition contains only EWG 1 grade components, it satisfies both functionality and safety, and is easy to apply to sensitive skin as it has no harmful effects.

EWG grade is a non-profit environmental civic group in the United States announced by the EWG (Environmental Working Group), which is a harmful component of cosmetic ingredients, and EWG conducts a thorough investigation into the harmfulness of cosmetic ingredients to classify the component harmfulness from grade 1 to grade 10, 1 Class 2 and Class 2 are classified as safety class.

The “Spicule” is a composite material composed of calcium and silicate in the form of various fine needles, which are usually collected from sponges living in fresh water in Europe and Russia, and can be generally obtained in the form of a powder. .

The fine needle is a fine needle-like structure that penetrates the skin layer to form a fine passage, so that the penetration effect of the applied cosmetic ingredient can be significantly increased. In addition, the fine needle may promote skin regeneration of collagen that acts on artificial wound healing and increase skin tissue temperature to increase bioactivity.

The fine needle is a material that is harmless to the human body and is not particularly limited as long as it is a powdery material that stimulates the skin to form fine pores and can be dissolved or discharged in the body.

The fine needle powder can be obtained from a spongy animal, the spongy animal being Spongilla lacustris L. , Spongilla fragilis Leidy and Epidatia fluviatilis ( Ephydatia fluviatilis ).

The sponge is a primitive marine organism that does not have muscles, nerves, or organs, and there are more than 5,000 species. The bone fragment is a tissue that functions as a skeleton having a needle shape existing in the body of an invertebrate animal, and is mainly composed of silicate and calcium carbonate. The spongy animal may include one-axis, three-axis, four-axis, or multi-axis bone fragments containing calcium carbonate or silicic acid as a main component.

The spongy animals inhabit the sea mainly in the form of sponges of various colors such as orange or turquoise, but freshwater spongy animals also exist.

The spongy animal is an organism in the initial stage of tissue with cells aggregated, and the body is composed of sponge fibers, bones forming body walls, and a group of cells bound to the bone fragments. The main component of the bone fragment is lime, silicic acid or silica and the like, and the size is about 100 μm, so it can be observed by a microscope.

The bone fragment is an effective means of skin regeneration, and can be used for correcting visual facial defects such as hyperpigmentation of various etiologies, micro wrinkles, damage caused by sunlight, and superficial scars, cotton, and enlarged pores. In addition, it has been utilized as a natural alternative approach to fine skin abrasion and skin abrasion, chemical dermabrasion, CO ₂ laser dermabrasion and erbium laser dermabrasion, which are physical procedures to improve skin condition.

When the bone fragments are brought into contact with the skin layer and rubbed, they physically separate to reduce keratinocyte aggregation, and detach the stratum corneum, sebum plug, and keratinocytes to form a micro-path, thereby maximizing the penetration effect of the cosmetic ingredient.

The “ Houttuynia cordata ” is also called Eoseongcho, and grows in a dense forest. The subterranean stem is elongated to the side and is thin and white. Stems stand upright, 20-50cm high, have several vertical lines, and smell. The leaves are alternate, 3-8cm long, pointed at the ends, flat at the edges, and the chin leaves are attached to the bottom of the petiole. The plant before flowering is used as a diuretic and anthelmintic, and is applied when the leaves are crushed and bitten by boils and poisons. In the private sector, it is used for swelling, purulent and hemorrhoids, and in oriental medicine, plants are used for gonorrhea, enteritis, urinary tract infection, pneumonia, and bronchitis.

The “ plantago asiatica ” is a perennial herb, elliptical or ovate, blooms white in June-August, and bears fruit of 6-8 black seeds in October. It is a herb native to East Asia and Central Asia, such as China and Japan, and has strong vitality and excellent fertility, as an old herb. When flowers bloom, dried ones are called chajeoncho, and seeds are called chajeon-cho, and they are also called pike, chajeon, Umochae, hamacho, mixed clam, baechagae, giljanggu, and yajichae. The tea planter, the fruit of plantain, is rich in sugar, mucilage, and fiber, and is used as a treatment for constipation and a diuretic in oriental medicine.

The “ Eclipta prostrate ” is an annual plant of the Chrysanthemum family that is 10 ~ 70cm in height. The stem grows straight, has bristles all over, and the branches emerge from the coexisting axilla, and again one branch grows at the end of the branch. The outpost contains saponin, nicotine, tannin, vitamin A, ecliptine, and various types of triphene compounds (α-terthienyl methanol and its acetate esters, etc.). On the leaves are weedelolactone and demethylwedelolactone-7-glucoside.

The "Shinsuncho" ( Angelica keiskei ) is a perennial plant belonging to the family Apiaceae, which contains not only vitamins, minerals, and dietary fiber, but also active substances such as germanium and vitamin B12, showing excellent effects on adult diseases and various diseases, mainly juice, powder, It is used in the form of health supplements such as tea.

The “extract” is an active ingredient contained in the extracted raw material by contacting the solvent and the extracted raw material under specific conditions, and may include all irrespective of the extraction method or the type of the component, as long as the substance contained in the raw material is separated from the natural product. .

For example, it is possible to include all those obtained by extracting a component soluble in a solvent from a natural product using water or an organic solvent, and a specific component such as an oil obtained by extracting only a specific component such as oil. The extract may be obtained by drying the powdered wheat, plantain, Hannyeoncho, and fresh herbs and powdering them, or by a variety of known extraction methods such as a hot water extraction method and an ethanol extraction method.

The extract is selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene glycol. Can be extracted with one or more solvents, specifically 60 to 80 v / v)% of ethanol.

The active ingredient contained in the raw material may have a different extraction ratio depending on the polarity of the solvent, and since the ethanol has excellent selectivity in extracting the bioactive substance of the natural raw material, an optimal skin improvement effect can be realized by the ethanol extraction. Can

Since the polarity of water and ethanol is different and the active ingredients extracted according to each polarity may be different, it is possible to appropriately control the concentration of the ethanol so that an optimal skin improvement effect can be realized. At this time, if the concentration of the ethanol is less than 60%, an active ingredient showing a skin improvement effect may not be sufficiently extracted, and if it is more than 80%, a proper yield may not be realized.

The extract is washed with water, dried and pulverized, followed by extraction and filtration by conventional methods such as reflux circulation extraction, pressure extraction, and ultrasonic extraction for 24 to 240 hours with a solvent having a volume of 10 to 20 times the weight of the raw material. Can be produced. In addition, the extract may be obtained in powder form by additional processes such as distillation under reduced pressure or freeze drying.

The extract may also include an extract that has been subjected to a conventional purification process. For example, the extract may be subjected to various purification methods additionally performed, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity, or affinity). It may include a fraction obtained through.

The extract may be extracted for 12 to 18 hours at a temperature of 50 ℃ to 60 ℃ using the ethanol as a solvent.

On the other hand, if the extraction temperature is less than 50 ℃, the extraction efficiency is lowered and the active ingredient may not be eluted appropriately. If it is more than 60 ℃, the active ingredient contained in the natural raw material may be deformed or lost at a high temperature, thereby reducing the skin improvement effect. Therefore, it is preferable to keep the extraction temperature within an appropriate range.

 In addition, if the extraction time is less than 12 hours, the active ingredient contained in the natural raw material may not be completely extracted, and if it exceeds 18 hours, the content of the residual active ingredient contained in the natural raw material is excessive, which is inefficient in terms of time and cost. Therefore, it is preferable to appropriately control the extraction time.

In one embodiment, the extract may be a fermentation extract prepared by inoculating a fermentation strain.

The "fermentation" refers to a process of decomposing organic substances using enzymes owned by the fermentation strain itself. It is known that the raw material that has undergone the fermentation process through the fermentation strain increases the effect of at least 2 to up to several tens of times than before fermentation. The fermented metabolites contain various amino acids, organic acids, and antioxidants that are good for the skin, and can promote hair growth to prevent or treat hair loss. In addition, through the fermentation process, the particles become smaller and the toxicity of heavy metals and the like decomposes, so that the absorption rate is excellent, and skin troubles and allergic side effects can be alleviated.

The “fermented extract” is sterilized with the medicinal wheat, plantain, medicinal herbs, and fresh herbs and fermented for a period of time at a certain temperature by inoculating the fermentation strain, and then the fermented medicinal wheat, plantain, medicinal herbs, and fresh herbs with specific solvent, preferably ethanol It can be prepared by extraction with.

The fermentation strain may be one or more mycelium selected from the group consisting of Pleurotusus ferulae , Ganoderma lucidum , Hericonium erinaceum and Phellinus linteus .

The above-mentioned “ Pleurotusus ferulae ” is rich in vitamins A, E, and D ₃ and has twice as many proteins as oyster mushrooms, and has high dietary fiber content, making it a good diet food. In addition, subsidiary mushrooms have about 6 times more vitamin content and higher content of unsaturated fatty acid linolenic acid, and high zinc and iron content, which are effective in preventing adult diseases and preventing aging.

The “ Ganoderma lucidum ” is a fruiting body of the oyster mushroom, and in general, Ganoderma lucidum , Ganoderma applanatum , Ganoderma tsugae , and Ganoderma tsugae -Ganoderma neo-japonicum , and both the porous bacteria and the basidiomycetes belonging to the genus Blocho. In addition, Yeongji is known as red paper, cock, wood paper, black paper, white paper, yellow paper, and blue paper. The extract obtained by extracting the fruiting body of Yeongji with water or an organic solvent exhibits a bitter taste (sweetness), and the components of this bitter taste are known to exhibit various physiological activities. These gourmet ingredients are rarely produced in liquid cultured mycelium, but are known to concentrate on the surface of the lampshade in fruiting bodies. These manor mushrooms are used as nutritional tonic, sedative, analgesic, anti-tumor and antihypertensive agents, or used as therapeutic agents such as arteriosclerosis, bronchitis, chronic arthritis and chronic hepatitis.

The “ hericium erinaceum ” is known to be rich in carbohydrates, proteins, amino acids, enzymes, inorganic salts and vitamins, and has an effect of enhancing anti-cancer and immune functions. In addition, a substance capable of being used as a dementia treatment agent was isolated from a mushroom, and it is known to have cancer cell proliferation effect of a hot water extract.

The “ Pellinus linteus ” is a rare rare perennial fungus that does not reproduce well. It is a white fungus belonging to the genus Mushroom (aphylloporales) and the fruiting body is made of wood. . The shape of the situation mushroom is maintained in the form of a clump of mud in the early stages, and after growing up, the shape of the mushroom is put out on the stump, so it is also called snow. Phellinus linteus has anticancer action because of the component called β-glucan contained in phellinus linteus, and β-glucan is a generic term for polysaccharides in which glucose is polymerized around β-1.3 chemical bonds. Many studies have been reported that beta-glucan stimulates humoral and cellular immunity in the human body to amplify the immune system and act as an immunomodulator.

The cosmetic composition may further include coffee silver skin extract.

The “coffee silver skin” is called silverskin, and is a silver cuticle that wraps around the center cut of coffee beans and green bean, which is endosperm. The coffee beans are commonly called beans, but are seeds of coffee trees. The coffee tree has a cherry-like red fruit that has a hard skin parchment when the fruit is peeled, and a silver skin and coffee beans that look like keratin when the fruit is peeled. The parchment and silver skin generated in the process of processing coffee beans are not used and are discarded.

According to a recent study, the coffee silver skin has a high content of flavonoids, high antioxidant power, and has no cytotoxicity against fibroblasts and keratinocytes in a stability test, but is harmful to harmful bacteria such as K. pneumoniae, S. epidermidis, and S.aureus. It has been reported to have antibacterial activity against (Francisca Rodrigues et al. 2015).

The cosmetic composition may further include a first complex extract composed of a medicinal extract, an medicinal extract, a mugwort extract, an Angelica extract, a black bean extract, and a peony extract.

The “ Swertia pseudo-chinensis ” is an annual or biennial grass. The root contains a strong bitter ingredient. The stems with hairs stand up straight and branch from the upper part. The stem is purple, and the height is about 15 ~ 30cm. The leaves are lanceolate, do not have petioles, and 2 sheets face each node. The flowers are gathered in the shape of a root at the end of the stem, and they have the habit of blooming from top to bottom. It has 5 oval petals. The diameter of the flower is 1.5 ~ 2cm, and it has a purple line on a white background, so it looks light purple. There are 5 stamens in the middle of the flower, and the pollen bag is dark purple. It is distributed only in Jeju Island and the southern region and grows on sunny meadows in the mountains and fields. Both leaves and stems, including flowers, are used as medicinal herbs. Picking and delicatessen When flowers are blooming, pick them up and dry them before sun drying. In Japan, it is also used as an anthelmintic drug such as a stomach-and-mouth medicine, an antidiarrheal agent, a roundworm, and a pinworm.

The “top baekpi” is a medicinal product made from the root bark of the Morus mulberry ( Morus alba L.) or the same plant, and its appearance is semi-tubular or banded and sometimes thinly cut vertically. The outer surface is white or yellowish brown with the main skin attached to it. The main skin is yellowish brown, easy to fall off, and has many fine vertical wrinkles and reddish brown skin. The transverse side is white or light brown, fibrous, and the inner side is dark tan and flat. Other names include Sanggeun Baekpi (桑根 白皮) and Baeksangpi (白 桑皮), and the fruit is called Sim. Epithelial skin treats seawater and asthma caused by waste heat and has a diuretic effect. It is used for acute pyelonephritis and fragile edema. It has a blood pressure lowering effect and is also used for nosebleeds and hemorrhage. It is also used for epidemic hepatitis. The pharmacological action has been reported by Jinhae, diuretic, blood pressure drop, sedation, analgesic, antipyretic, antispasmodic, antibacterial, etc.

The “wormwood ( Artemisia princeps var )” is called a leaf in oriental medicine and has been used in various ways for food and medicinal purposes. In particular, mugwort has warm properties, so it is useful for the treatment of abdominal pain by strengthening the functions of the stomach, liver, and kidney. It removes waste products from blood vessels to prevent hypertension and arteriosclerosis, improves immune function and detoxification, improves liver function, and prevents aging It is known to have various effects. In particular, the mugwort contains vitamin A, vitamin B1, vitamin B2, vitamin C, amino acids, polyphenols and flavonoids, and thus removes free radicals and soothes the scalp, and can give the hair shine and elasticity.

The " Angel Angelica gigas Nakai " is a root of the Angelicagigas NAKAI belonging to the Apiaceae family, and contains a large amount of coumarin components such as decursin and decursinol. The Angelica has pain relief, drainage, haemostasis, blood retention, and tonicity, and is known to promote blood circulation to make the scalp and hair follicles healthy.

The “black soybean” is an annual herb belonging to the leguminosae family , and its origin is from northeastern China to Huabei and is cultivated worldwide. It is known that black beans are effective for coughing, bronchial, and asthma in oriental medicine, have excellent detoxification effect, and have various effects such as anti-aging and intestinal relaxation. In particular, since the black bean contains a large amount of vitamin E and unsaturated fatty acids, the blood vessels of the peripheral blood vessels may be improved by expanding blood vessels to promote hair growth.

The "peony" is a perennial plant of the peony family ( Paeoniaceae ). In Chinese medicine, the root is washed or dried in boiling water, then taken out and dried. The peony or peony is called the peony root. The peony root is called the peony root. It is said that the Peeled Peel was. The peony contains active ingredients such as pioniflorine, pioneel, pionein, benzoic acid, essential oil, resin, tannin, and triterpenoid, and is known to have various effects and effects such as skin whitening and antioxidant. In particular, the peony protects the hair from external damage, provides moisture to the scalp, and is reported to induce hair growth.

In one embodiment, the cosmetic composition may further include a second complex extract consisting of ginkgo biloba extract, sewage extract, centella extract, green tea extract and aloe vera extract.

The "Ginkgo leaf" is extracted from the ginkgo ( Ginko biloba L. ), a deciduous tree belonging to the ginkgo family, and the ginkgo leaf has free radical (harmful oxygen) removal, antioxidant, cell membrane protection, blood vessel disease improvement, blood pressure lowering effect, etc. It contains flavonoids and various substances that improve blood circulation, remove blood clots, prevent blood aging, suppress skin aging, protect dry skin, and prevent fine lines on the face. In addition, it has an anti-allergic effect as well as an antibacterial effect. In general, ginkgo biloba extract is a comprehensive component composed of the flavonoids and aromatic oxycarboxylic acids of leaves, and the active ingredients such as flavone glycosides, ginconic acid, gincogetin, gincoide A, B, C, M, and telfenlactones. It contains.

The “polygonum multiflorum Thunberg ” is a tendril and perennial plant that grows about 1 to 3 m long, and the stems are rolled up as the branches are split, the roots extend into the ground, and show the characteristics of making round tuber roots. The leaves are egg-shaped, 3-6 cm long, 2.5-4.5 cm wide, and have a flat edge. Together with ginseng and wolfberry, it has been regarded as one of Gangjeong's three herbal medicines, and is known to have a function of blackening hair.

Said “ Centella asiatica ” is a perennial plant belonging to the mountain family. It is also called Gotucola, Tiger Grass, Clam Grass or Horseshoe Grass and is often native to the mountains and fields of the southern islands of the Korean Peninsula. The main stem extends to the side, and there are two degenerated scale-like leaves near the root node. The leaf is dense at the node, elongated, 2 ~ 5cm in diameter, the surface is shiny, there are dull teeth on the edges, and the petiole is 4 ~ 20cm long. The flowers are reddish-purple, and 3 ~ 4 flowers on the leaf axil, in mountain-shaped inflorescence, are shaped like a head. The pistil is two-pronged, and the fruit is a round shape of the subfamily. Centella asiatica, a component of Madecassol, is used as a soothing ingredient to soothe weakened skin due to irritation and to strengthen its own vitality.

The "green tea" refers to the leaves of a dried tea tree that has been roasted as it is without fermentation. Tea tree ( Camellia sinensis ) is a perennial evergreen tree belonging to the Camellia family, and is widely cultivated in subtropical regions such as Africa and Russia, mainly in Korea and Asia. The main components of green tea are catechin, caffeine, protein, free amino acid, starch, fiber, pectin, chlorophyll, and flovonol derivatives. Green tea is known for its anti-cancer effect, prevention of hypertension and arteriosclerosis, anti-diabetes effect, and anti-obesity effect.

The “Aloe Vera ( Barbados aloe )” is native to the Mediterranean and Africa. The gel-like sap contained in the leaves is known to be effective in treating burns and arthritis. As the first record in the Orient, it was mentioned as a presbytery in Gaeboboncho (Gaebosinsangjeongboncho) compiled during the Song Dynasty in the mid-10th century, and was first mentioned in Donguibogam in Korea. It is a perennial perennial plant. Its stem is 30-60cm high, short and grows up to 100cm. A lot of long, hard leaves come out of the stem. The leaves are 15 ~ 40cm long and 2 ~ 7cm wide, with light green background with white spots, thick, fleshy, succulent. The bottom is wider and narrower as it goes up, the back is rounded, and the front is slightly indented. There are serrated small gray thorns on the edges. There is a slight groove at the tip of the leaf, and the tip is sharp. Flowers that look like small coffins bloom in winter and spring. The flowers are bright yellow or orange in color, forming a gunshot wound and blooming downward. It is easy to cultivate, resistant to pests, and has excellent oxygen production at night, making it a good indoor air purification plant. It is also suitable for indoor cultivation in bedrooms, living rooms, offices, etc., and is widely used as a medicinal plant. It is used for various uses such as raw materials for cosmetics and pharmaceuticals, food, etc., and has been found to be effective in neutralizing toxins due to sterilization and recovery, fatigue and skin neutralization, fever, constipation, and ulcers. Vitamin A, C, D, E, B12, fatty acids, amino acids, selenium, calcium and aloin (aloin), aloesine (aloesin) and contains about 300 different ingredients. India, North Africa, and Manila have very large distribution areas.

In one embodiment, the cosmetic composition may be for preventing hair loss and promoting hair growth.

The term “hair loss” refers to an increase in the number of abnormal hair loss due to a shorter hair growth rate and more hair in the degenerative or resting period. It has been reported that hair loss is associated with the death and dropping of hair follicles, and that male hormones play a major role in hair loss and dropping.

The "hair loss prevention" refers to controlling the action of an enzyme involved in testosterone, the male hormone, and preventing abnormal hair loss by activating the dermal papilla cells.

The "hair" refers to the hair of the body, and refers to a thread-like form composed of keratin protein. Specifically, it may be hair, eyebrows, eyelashes, nose hair, or body hair, but is not limited thereto.

The present inventors confirmed that the 5-alpha-reductase activity inhibitory effect (Table 3), hair growth promoting effect (Table 4), and hair growth function (Table 6) appeared remarkably excellent when the sample of the present invention experiment was processed, from which It was confirmed that the samples of the examples of the present invention can exhibit preventive and therapeutic effects on hair loss and promote hair growth.

The cosmetic composition may be for suppressing inflammation of the scalp.

The "inflammation" is a mechanism for restoring and regenerating the damaged site when an invasion that causes any organic changes such as physical action, chemical substances, or bacterial infection is applied to a living body or tissue, and once irritation is applied, histamine is locally applied. Vascular active substances such as serotonin, bradykinin, prostaglandin, hydroxyeicosatetraenoic acid (HETE), and leukotriene are liberated and inflammation may be caused by increased vascular permeability.

The present inventors confirmed that prostaglandin E2 (PGE2), which is the cause of inflammatory hair loss, was significantly suppressed (Table 7) when treating the sample of the experimental example of the present invention, and it was possible to prevent and treat hair loss by inhibiting the inflammatory response in the scalp. Was confirmed.

The cosmetic composition is hair shampoo, hair conditioner, hair conditioner, hair treatment, hair tonic, scalp treatment, hair lotion, hair cream, hair mist, hair spray, hair ampoule, hair pack, hair essence, hair oil, scalp scaler , Can be formulated with one or more selected from the group consisting of hair nutrient longevity and general ointment.

The cosmetic composition may be formulated by a conventional method. Reference may be made to Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA in the formulation of external preparations for skin, and in the formulation of cosmetic compositions, the International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared as a general emulsifying formulation and a solubilizing formulation. For example, lotion such as flexible lotion or nutrition lotion; Emulsions such as facial lotions and body lotions; Creams such as nutrition cream, moisture cream, and eye cream; essence; Cosmetic ointment; spray; Gel; pack; Sunscreen; Makeup base; Liquid type, solid type or spray type foundation; powder; Makeup removers such as cleansing cream, cleansing lotion, and cleansing oil; Or it may be formulated with a cleaning agent such as cleansing foam, soap, body wash, but is not limited thereto. In addition, the external preparation for skin may be formulated as an ointment, patch, gel, cream or spray, but is not limited thereto.

The cosmetic composition may be appropriately blended with other ingredients within a range that does not impair the purpose according to the present invention, depending on the type of formulation or purpose of use, in addition to the essential ingredients in each formulation.

The cosmetic composition may include an acceptable carrier, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, and the like can be appropriately blended, but is not limited thereto. no.

The acceptable carrier may vary depending on the formulation. For example, when formulated as ointments, pastes, creams or gels, animal oils, vegetable oils, waxes, paraffins, starch, trakant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or their Mixtures can be used

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or a mixture thereof may be used as a carrier component, and in the case of a spray, chlorofluorohydro Propellants such as carbon, propane, butane or dimethyl ether may be further included.

When formulated as a solution or emulsion, the cosmetic composition may use a solvent, solubilizer, or emulsifier as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Btyl glycol oil can be used.

When formulated as a suspension, the cosmetic composition is a liquid diluent such as water, ethanol or propylene glycol as a carrier component, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used.

The cosmetic composition according to the quality or function of the final product, fatty substances, organic solvents, solubilizers, thickening agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances commonly used in the industry , Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion blockers, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, commonly used in cosmetics It may further contain adjuvants commonly used in the cosmetic or dermatological field, such as any other ingredient.

However, the adjuvant and the mixing ratio thereof may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

Through the following examples, it will be apparent that the present invention is not limited to the present invention by the following examples.

Preparation Example 1: Preparation of fine needle powder

800 g of a 0.5N-sodium hydroxide solution was added to 200 g of a natural sponge obtained from dried sponge ( Spongilla lacustris ), followed by mixing and grinding in an ultrasonic process. After stirring, impurities were removed by centrifugation, and 700 ml of purified water was added to remove soluble impurities and sodium hydroxide solution.

A trace amount of sodium hydroxide was removed by chemical reaction with 800 ml of a 2% -L-ascorbic acid solution, and the over-dosed L-ascorbic acid solution was removed and dried to obtain a fine needle powder from which purified water was removed.

Preparation Example 2: Preparation of natural extracts

After washing the weak wheat, plantain, Hannyeoncho and fresh herbs, they were completely dried at room temperature and pulverized to obtain 100 g of each pulverized product.

After washing the coffee silver skin, it was dried at room temperature and pulverized to obtain 100 g of a pulverized product.

After washing the medicinal sugar, Sangbaekpi, mugwort, Angelica, black bean and peony, they were dried at room temperature and pulverized to obtain 100 g of each pulverized product and mixed to prepare a raw material for the first complex extract.

After rinsing ginkgo biloba, sorghum, coriander, green tea, and aloe vera, they were dried and crushed at room temperature to obtain 100 g of each pulverized product and mixed to prepare a raw material for the second composite extract.

For each pulverized material and raw material, 10 times the volume of ethanol aqueous solution (70% w / w) as a solvent was immersed and extracted at a temperature of 50 ° C. for 12 hours.

After filtering the extract with a 250 mesh and 0.5 μm filter, the remaining raw materials were repeatedly extracted three times in the same manner, concentrated under reduced pressure at 30 ° C., and freeze-dried to obtain a solid content of the natural extract.

Preparation Example 3: Preparation of fermented extract

After washing the weak wheat, plantain, Hannyeoncho and fresh herbs, the mixture was completely dried at room temperature and pulverized to obtain 100 g of a pulverized product.

The crushed product was autoclaved at 121 ° C. for 50 minutes and then inoculated with 10% (w / v) mycelium of Pleurotusus ferulae , a fermentation strain.

After culturing the pulverized inoculated fermentation strain at 30 ° C. for 12 days, the fermented pulverized product was immersed for 7 days at room temperature using 10 times the volume of ethanol as a solvent.

The extract was filtered through a 250 mesh and 0.5 μm filter, and the remaining raw materials were repeatedly extracted three times in the same manner, concentrated under reduced pressure at 50 ° C., and freeze-dried to obtain a solid content of the first complex fermentation extract.

Preparation Example 4: Preparation of fermented extract

A fermented extract was prepared in the same manner as in Preparation Example 3, but only the fermented strain was changed to Ganoderma lucidum .

Preparation Example 5: Preparation of fermented extract

A fermentation extract was prepared in the same manner as in Preparation Example 3, but only the fermentation strain was changed to roe mushroom ( Hericium erinaceum ).

Preparation Example 6: Preparation of fermented extract

A fermented extract was prepared in the same manner as in Preparation Example 3, but only the fermented strain was changed to Phellinus linteus .

Comparative manufacturing example: Preparation of fermented extract

A fermentation extract was prepared in the same manner as in Preparation Example 3, but only the fermentation strain was changed to Lactobacillus plantarum .

Examples and comparative examples

Examples and comparative examples were set by mixing the samples as shown in Tables 1 and 2 below to verify the effect of preventing hair loss and promoting hair growth of the obtained extract and fermentation extract.

[Content (parts by weight)] division Example One 2 3 4 5 6 7 8 9 10 11 12 13 Fine needle powder 200 200 200 200 200 200 200 150 150 150 150 150 120 Yakmomil extract 200 - 50 - - - - 50 50 50 40 40 40 Plantain extract - 100 50 - - - - 50 50 50 40 40 40 Hannyeoncho Extract - - 50 - - - - 50 50 50 40 40 40 Fresh green tea extract - 100 50 - - - - 50 50 50 40 40 40 Ferment extract (Production Example 3) - - - 200 - - - - - - - - - Fermented extract (Production Example 4) - - - - 200 - - - - - - - - Ferment extract (Production Example 5) - - - - - 200 - - - - - - - Fermented extract (Production Example 6) - - - - - - 200 - - - - - - Coffee Silverskin Extract - - - - - - - 50 - - 50 50 40 1st complex extract - - - - - - - - 50 - 40 - 40 Second Complex Extract - - - - - - - - - 50 - 40 40

[Content (parts by weight)] division Comparative example One 2 3 4 5 6 7 8 9 Fine needle powder 400 - - - - - - - - Yakmomil extract - 400 - - - - - - - Plantain extract - - 400 - - - - - - Hannyeoncho Extract - - - 400 - - - - - Fresh green tea extract - - - - 400 - - - - Fermented extract (comparative manufacturing example) - - - - - 400 - - - Coffee Silverskin Extract - - - - - - 400 - - 1st complex extract - - - - - - - 400 - Second Complex Extract - - - - - - - - 400

Experimental Example 1: Skin safety test

In order to verify the skin safety for the samples of Examples and Comparative Examples, MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT).

After the samples of Examples and Comparative Examples were suspended in purified water according to concentrations of 0, 1, 5, 10, 20, 50 and 100 mg / L, cell viability was measured.

Cytotoxicity was performed by modifying Mosmann's method of measuring cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

Fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) were respectively dispensed into 96-well plates at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C., 5% CO ₂ for 48 hours. Did.

The culture medium was removed and the sample was incubated in the medium treated for each concentration for 48 hours, and then the medium was removed and washed repeatedly with PBS (Phosphate buffered saline).

MTT was dissolved in PBS at 5 mg / mL, 50 L was added, and cultured at 37 ° C and 5% CO ₂ for 48 hours. Dimethyl sulfoxide (DMSO) was added in 100 L per well, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of the measurement, cytotoxicity was not confirmed at all concentrations of each sample. The results suggest that the sample is not only harmless to the human body but also has excellent human safety.

Experimental Example 2: 5 alpha-reductase (5α-reductase) inhibitory activity test

The inhibitory capacity of the above-mentioned examples and comparative examples for 5 alpha-reductase, which is known as the main cause of hair loss, was evaluated.

Rat dermal papilla cells (DPCs, Dermal papilla cells) were divided into 24 well plates at a concentration of 5 x 10 ⁵ cells / well and cultured for 24 hours.

Samples of the above Examples and Comparative Examples were treated in each well at a concentration of 50 mg / L and stimulated with testosterone at a concentration of 50 nM after 1 hour. The supernatant was separated from the incubated solution to obtain DPCs pellets.

In the following procedure, the concentration of dihydrotestosterone (DHT) was measured using an enzyme-linked immunosorbent assay (ELISA).

The DPCs pellet was coated with 100 μL of DPC ELISA microtiter strips coated with Rabbit Anti-DHT antibody, and 25 μL of DHT antiserum was dispensed into each well, reacted at 2 to 8 ° C. for 15 to 20 hours, and washed 3 times.

DHT-HRP Enzyme Conjugate was dispensed into each well by 100 μL, reacted at room temperature for 30 minutes on a shaker, and washed 3 times. After dispensing 100 μL of the substrate and reacting at room temperature for 20 to 30 minutes, a stop solution of 100 μL was treated to measure the absorbance at 450 nm, indicating the amount of DHT production. The amount of DHT produced relative to the control group not treated with the sample was calculated as the inhibition rate (%), and the results are shown in Table 3 below.

division 5 Alpha-reductase inhibition rate (%) Example 1 45 Example 2 54 Example 3 53 Example 4 55 Example 5 54 Example 6 53 Example 7 52 Example 8 56 Example 9 57 Example 10 55 Example 11 60 Example 12 59 Example 13 61 Comparative Example 1 23 Comparative Example 2 26 Comparative Example 3 31 Comparative Example 4 26 Comparative Example 5 29 Comparative Example 6 27 Comparative Example 7 28 Comparative Example 8 30 Comparative Example 9 31

Referring to Table 3, in the medium treated with the sample of the above example, the activity of the 5 alpha-reductase was effectively inhibited. The result shows that the sample of the example inhibits the production of dihydrotestosterone, thereby causing the activity of the 5 alpha-reductase. It suggests that it is possible to achieve excellent hair loss improvement or hair growth effect by inhibiting.

Experimental Example 3: Confirm the effect of promoting hair growth

C57BL / 6 mouse model was used as the animal test model used to evaluate the growth of hair. The C57BL / 6 mouse has a black body hair, has a characteristic of spontaneous alopecia, and melanocytes are present only in the hair follicle, and melanin synthesis is a hair growth cycle. It is well-matched with and has the advantage of determining the growth cycle of hair by skin color, and thus is widely used for evaluation of hair growth promotion.

In the hair growth promotion evaluation test, the hairs of the back were removed using 42-56-day-old mice (C57BL / 6, male), and 7 of each of Examples 1 to 13 and Comparative Examples 1 to 9 were applied to the back of each individual. 100 μL was applied once a day for 28 days. 30% ethanol was applied as a negative control. Table 4 below shows the results by analyzing the ratio of the area where the new hair grew over the area where the hair was removed.

sample Area ratio of area where new mothers grew / are removed hair (%) 9 days later 20 days later Negative control 4.12 55.92 Example 1 15.56 91.46 Example 2 14.51 90.30 Example 3 16.11 92.55 Example 4 17.91 92.67 Example 5 17.75 93.54 Example 6 17.84 93.66 Example 7 17.43 92.91 Example 8 17.43 92.57 Example 9 18.10 94.53 Example 10 16.63 92.07 Example 11 20.30 95.81 Example 12 20.50 95.72 Example 13 20.47 96.01 Comparative Example 1 9.77 83.42 Comparative Example 2 8.82 80.89 Comparative Example 3 9.56 78.87 Comparative Example 4 8.15 77.56 Comparative Example 5 8.13 78.10 Comparative Example 6 8.25 77.94 Comparative Example 7 9.10 77.53 Comparative Example 8 8.80 76.91 Comparative Example 9 9.12 78.01

Referring to Table 4, the samples of Examples 1 to 13 had better hair growth promoting effects than Comparative Examples 1 to 9. In particular, Examples 9, 11, and 13 further comprising the first composite extract were other examples. Compared to, the effect of promoting hair growth was further improved.

Experimental Example 4: Hair growth functional evaluation test

In order to evaluate the hair growth function of the samples obtained in the above Examples and Comparative Examples, the following clinical trials were conducted on adult men and women who had hair loss symptoms.

A total of 115 males and females aged 30 to 60 are divided into 23 groups, and commercially available minoxidil 5% products and liquid tonics prepared from samples of the examples and comparative examples are administered once a day (5 mL per session) for 3 months. It was applied to the result was observed.

The evaluation of the effect was evaluated in five stages by evaluating the average daily hair loss during tricycle, the visual judgment findings, and the degree of improvement in subjective symptoms.

At this time, each evaluation scale was set out of 5 points, and each grade was set in units of 1 point. The detailed evaluation criteria are shown in Table 5 below, and the evaluation results according thereto are shown in Table 6 below.

score Visual judgment Subjective symptoms Hair loss in tricycle One worse worse increase 2 maintain maintain maintain 3 Slightly improved Slightly improved Small reduction 4 Moderate improvement Moderate improvement Moderate reduction 5 Drastically improved Drastically improved Mass reduction

division Visual judgment Subjective symptoms Hair loss in tricycle Overall improvement diagram Example 1 3.6 3.8 3.8 3.73 Example 2 3.7 3.7 3.7 3.70 Example 3 3.8 4.0 3.8 3.87 Example 4 4.0 3.9 3.7 3.87 Example 5 3.9 4.0 4.0 3.97 Example 6 4.0 3.9 4.0 3.97 Example 7 4.1 3.9 3.9 3.97 Example 8 3.9 4.0 4.1 4.00 Example 9 4.1 4.0 4.1 4.06 Example 10 3.9 4.1 3.9 3.97 Example 11 4.1 4.2 4.2 4.17 Example 12 4.1 4.2 4.1 4.13 Example 13 4.2 4.2 4.2 4.20 Comparative Example 1 3.1 3.3 3.2 3.20 Comparative Example 2 3.2 3.4 3.3 3.30 Comparative Example 3 3.1 3.3 3.2 3.20 Comparative Example 4 3.1 3.5 3.1 3.23 Comparative Example 5 3.2 3.1 3.4 3.23 Comparative Example 6 3.1 3.4 3.2 3.23 Comparative Example 7 3.3 3.1 3.3 3.23 Comparative Example 8 3.1 3.1 3.3 3.17 Comparative Example 9 3.3 3.4 3.1 3.27 Minoxidil 4.1 4.1 4.2 4.13

Referring to Table 6, the samples of the Examples exhibited an equivalent effect in the amount of hair loss during visual judgment, symptoms, and hair washing compared to the control group minoxidil treatment group. It suggests that it is possible to realize superior effects compared to commercial products and to improve the overall satisfaction of users.

Experimental Example 5: Prostaglandin E2 (PGE2) inhibitory activity test

Inhibition of the activity of prostaglandin E2 (PGE2) was able to prevent hair loss due to inflammation, thus confirming the inhibitory effect of PGE2 production in the samples of Examples and Comparative Examples. As a measuring instrument, a Prostaglandin E2 EIA Kit was used.

After treating the samples of the examples and comparative examples with RAW 264.7 cells at a concentration of 0.1% LPS and 50 mg / L for 24 hours, the cell culture supernatant was loaded 50 μL into a 96-well plate coated with goat anti-mouse, and here 50 μL of the primary antibody solution and 50 μL of the PGE2 conjugate were added thereto, and reacted at 4 ° C. for 18 hours.

After washing 5 times with the washing solution, and reacting with 200 μL of Ellman's reagent for 60 minutes, the absorbance was measured at 405-420 nm, and the results are shown in Table 7 below. It was expressed as a relative value (%) to the amount of PGE2 produced in the negative control group without adding the sample.

division PGE2 production rate (%) Example 1 64 Example 2 61 Example 3 53 Example 4 54 Example 5 54 Example 6 53 Example 7 52 Example 8 46 Example 9 44 Example 10 48 Example 11 42 Example 12 43 Example 13 41 Comparative Example 1 84 Comparative Example 2 84 Comparative Example 3 83 Comparative Example 4 88 Comparative Example 5 87 Comparative Example 6 86 Comparative Example 7 81 Comparative Example 8 83 Comparative Example 9 82

Referring to Table 7, the amount of PGE2 produced by LPS stimulation was significantly reduced by the sample of the above example. The result shows that the sample of the above example effectively suppresses the inflammatory response caused by prostaglandin E2, thereby exhibiting excellent hair loss improvement effect. It suggests that you can.

The above description of the present invention is for illustration only, and a person having ordinary knowledge in the technical field to which the present invention pertains can understand that it can be easily modified to other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the above-described embodiments are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all modifications or variations derived from the meaning and scope of the claims and equivalent concepts should be interpreted to be included in the scope of the present invention.

Claims (9)

Fine needle powder; And
Cosmetic composition for preventing hair loss and promoting hair growth, including as an active ingredient; one or more extracts selected from the group consisting of medicinal wheat extract, plantain extract, Hannyeoncho extract and fresh herb extract. According to claim 1,
The extract is a cosmetic composition for preventing hair loss and promoting hair growth, which is a fermented extract prepared by inoculating a fermentation strain. According to claim 2,
The fermentation strain is a cosmetic composition for preventing hair loss and promoting hair growth, which is one or more mycelium selected from the group consisting of Pleurotusus ferulae , Ganoderma lucidum , Hercobium erinaceum and Phellinus linteus . According to claim 1,
The cosmetic composition is a cosmetic composition for preventing hair loss and promoting hair growth further comprising a coffee silver skin extract. According to claim 1,
The cosmetic composition is a cosmetic composition for preventing hair loss and promoting hair growth, further comprising a first complex extract composed of a medicinal extract, an medicinal extract, a mugwort extract, an Angelica extract, a black bean extract and a peony extract. According to claim 1,
The cosmetic composition is a cosmetic composition for preventing hair loss and promoting hair growth, further comprising a second complex extract consisting of ginkgo biloba extract, sewage extract, centella extract, green tea extract and aloe vera extract. delete According to claim 1,
The cosmetic composition is a cosmetic composition for preventing hair loss and promoting hair growth, further comprising a scalp inflammation inhibitory use. The method according to any one of claims 1 to 6,
The cosmetic composition is hair shampoo, hair conditioner, hair conditioner, hair treatment, hair tonic, scalp treatment, hair lotion, hair cream, hair mist, hair spray, hair ampoule, hair pack, hair essence, hair oil, scalp scaler , Cosmetic composition for preventing hair loss and promoting hair growth, formulated with at least one selected from the group consisting of hair nutrient makeup and general ointment.