KR20130045451A - Cosmetic composition containing croton lechleri resin extract - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a Croton Lechleri resin extract is provided to improve skin moisturization, elasticity, anti-aging, and skin whitening, and to ensure antibacterial and anti-inflammatory effects. CONSTITUTION: A cosmetic composition contains 0.0001-20 wt% of a Croton Lechleri resin extract as an active ingredient. The extract contains proanthocyanidin and taspine as active ingredients. The extract is prepared by leaching Croton Lechleri resin in water or an organic solvent, purifying, and extracting. [Reference numerals] (AA) Example 1; (BB) Comparative example 1; (CC) 0 week; (DD) 2 weeks; (EE) 4 weeks

Description

Cosmetic composition containing Dragon Blood resin extract {Cosmetic composition containing Croton Lechleri resin extract}

The present invention relates to a cosmetic composition that can improve the overall skin condition by containing a dragon blood resin extract as an active ingredient.

Skin protects the human body from external physical and chemical stimuli and is divided into three layers: epidermis, dermis and subcutaneous fat tissue. In particular, the skin contains about 65-70% of the moisture that the human body has, which carries various bioactive substances necessary for the human body and helps keep the skin moist. The skin also plays an important role in controlling the evaporation of this moisture out of the body.

The epidermis is divided into stratum corneum, granule layer, play layer and basal layer from the outside. The stratum corneum of the epidermis contains about 10-20% of moisture and is located at the outermost part of the human body, forming a natural protective film made of sebum from the sebaceous glands and sweat from the glands, preventing evaporation of moisture from outside the body. Blocks material infiltration. The cells constituting the stratum corneum have a high concentration of natural moisturizing factor (NMF), which is a water-soluble ingredient, which helps the skin to show flexibility and maintain proper moisture. However, ultraviolet rays, excessive physical and chemical stimuli, stress, and malnutrition received from the outside degrade the normal function of the skin and promote skin aging such as loss of elasticity, keratinization, and wrinkle formation. In particular, keratinocytes are made in sequence and the outermost layers of old keratinocytes sometimes deviate, so if the keratinization process that maintains a constant thickness of the epidermis is not smooth, abnormal keratinocytes are formed and the stratum corneum is thickened on the skin surface. The skin becomes rough and dull, fine wrinkles are generated, and eventually skin aging progresses. In addition, the reduction of biosynthesis of type 1 procollagen through the biosynthesis of MMP-1 by UV rays related to the decomposition of free radicals and skin substrates produced by UV rays, and the decrease in fission ability of fibroblasts present in the dermal layer of skin are also associated with skin aging. There is this.

United States Patent Application No. 2002-494567

Accordingly, the present inventors have tried to find a raw material having a skin condition improvement effect without causing skin side effects among the substances derived from nature, and found that the Dragon Blood resin extract is very effective in improving the overall skin condition and completed the present invention. .

Accordingly, an object of the present invention is to improve the overall skin condition such as skin moisturizing, elasticity improvement, anti-aging, whitening effect, anti-acne, anti-inflammatory acne improvement, pore reduction, sebum reduction, acne improvement, keratin trimming effect, etc. It is to provide a composition.

In order to achieve the above object, the present invention provides a cosmetic composition containing a dragon blood resin extract as an active ingredient.

The cosmetic composition of the present invention contains a dragon blood resin extract as an active ingredient to reduce the biosynthesis of MMP-1 by ultraviolet light related to the growth promoting effect of keratinocytes, the active oxygen scavenging effect produced by ultraviolet light, and the decomposition of the skin matrix. Since the effect of promoting the biosynthesis of type 1 procollagen and the whitening effect through promoting the production of melanin produced is excellent, it can be usefully used as a cosmetic composition for anti-aging and skin moisturizing enhancement. In addition, it has excellent antibacterial and anti-inflammatory effects, which can improve acne symptoms, reduce pores, reduce sebum, and turnover of keratin smoothly.

1 is a graph showing the result of measuring the moisturizing power of the composition according to the present invention by the method of measuring transdermal moisture loss (TEWL).
Figure 2 is a graph showing the result of measuring the moisture content of the skin by the composition according to the present invention with a conniometer.

The present invention provides a cosmetic composition containing a dragon blood resin extract as an active ingredient.

Hereinafter, the present invention will be described in more detail.

Dragon Lechleri used in the present invention is a 10-20 m giant native to Peru and inhabits the region of South America upstream of the Amazon. The tree's resin, named Dragon's Blood because of its red color, has been a traditional remedy since ancient times.

Dragon Lechleri extract contains proanthocyanidin and taspine as active ingredients. Proanthocyanidins are a type of flavonoids that have a savory taste and color in vegetables, fruits, and wines. Taspin is an organic compound that contains nitrogen and is known to have a beneficial effect on the skin such as wound healing.

Dragon Blood resin extract used in the present invention is preferably contained in 0.0001 ~ 20.0% by weight based on the total weight of the composition. If the content is less than 0.0001% by weight, the effect is insignificant, and if the content exceeds 20.0% by weight, there is a problem of the feeling of use of the product and stability of the formulation.

Dragon blood resin extract used in the present invention can be prepared by a conventional production method in the art, the production method is not particularly limited. For example, it can be obtained by a general method of leaching, purifying and separating a dragon blood resin in water or an organic solvent. At this time, the organic solvent used is not particularly limited, and may be C ₁ to C ₅ lower alcohol, ether, ethyl acetate or chloroform. The lower alcohol of C ₁ to C ₅ may be, for example, one or two or more mixed solvents selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol. .

The cosmetic composition according to the present invention has excellent growth promoting effect of keratinocytes, active oxygen scavenging effect produced by ultraviolet rays, type 1 procollagen biosynthesis promoting effect through reduction of biosynthesis of MMP-1 by ultraviolet rays related to skin matrix degradation, and It is excellent in inhibiting tyrosinase activity and shows excellent skin anti-aging and moisturizing effect. Therefore, the composition of the present invention is a composition for promoting keratinocyte growth, a composition for enhancing skin antioxidant, reducing the biosynthesis of MMP-1, a composition for promoting type 1 procollagen biosynthesis, a composition for promoting skin whitening, a composition for preventing skin aging, and skin It can be usefully used in cosmetics as a moisturizing enhancing composition.

In addition, the cosmetic composition according to the present invention is excellent in anti-inflammatory and antibacterial effect, and thus effective in improving acne. In addition, it can be usefully used to reduce skin pores, reduce sebum, and turnover of keratin smoothly to prepare skin texture.

The cosmetic composition according to the present invention may contain, in addition to the above-mentioned substances, other components that can give a synergistic effect to the main effect, preferably within the range of not impairing the main effect of the present invention. In addition to the active ingredient, other ingredients may be appropriately selected and blended by those skilled in the art according to the formulation or use purpose of other cosmetics.

For example, the cosmetic composition may contain a skin absorption promoting substance to increase the effect. In addition, the cosmetic composition of the present invention may include a material selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract. In addition, the cosmetic composition of the present invention may include other ingredients which are usually formulated into cosmetics as necessary in addition to the essential components, and examples thereof include fats and oils, humectants, emollients, surfactants, organic and inorganic pigments. Organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, blood circulation accelerators, cooling agents, limiting agents, purified water and the like.

Other compounding components that can be included in the cosmetic composition of the present invention is not limited thereto, and the compounding amount of the above components is possible within a range that does not impair the object and effect of the present invention.

The cosmetic composition according to the present invention is not particularly limited in the formulation, for example, softening longevity, astringent longevity, nourishing longevity, nourishing cream, massage cream, eye cream, eye essence, essence, cleansing cream, cleansing lotion, cleansing foam Formulations such as cleansing water, packs, powders, makeup bases, foundations, lipsticks, body lotions, body creams, body oils, body essences, body cleansers, essences, soaps, emulsions, lotions, ointments, gels, creams, patches or sprays Can be mentioned.

Hereinafter, the present invention will be described in more detail by way of Examples and Test Examples, but the present invention is not limited thereto.

Test Example 1 Growth Effect of Keratinocytes-MTT Analysis

The effect of Dragon Blood resin extract on the growth ability of keratinocytes was measured by MTT method. In order to compare the effect on the growth ability of keratinocytes, the growth ability of keratinocytes was also measured and compared with Centella asiatica Extract.

Dragon Blood resin extract was purchased from Naturex (NATUREX, USA) and Centella asiatica extract was purchased from Bioland (South Korea).

First, the keratinocytes were planted in a 96 well plate at a concentration of 5 × 10 ³ per 200 μl per well and incubated for 24 hours, and then the dragon blood resin extract and the centrifugal extract were 0.001, 0.01 and 0.1%, respectively. After incubation for 48 hours, the culture solution was aspirated and removed, washed once with PBS and MTT (Methylthiazolyldiphenyl-tetrazolium bromide, Sigma, USA) 0.5 mg / ml solution was added to the cells in 100 μl each, followed by 4 hours. Incubated at 37 ° C., 5% CO ₂ . Thereafter, the culture solution was aspirated and removed, and then 200 µl of DMSO (dimethyl sulfoxide) solution was added, shaken for 10 minutes in a shaker, and the absorbance at 540 nm was read with an ELISA reader (DIbiotech, Korea). . At this time, the control group was used as the untreated group of dragon blood resin extract and centella extract, the results are shown in Table 1 below.

density(%) Absorbance Dragon Blood Resin Extract 0.001% 153 0.01% 163 0.1% 170 Centipede extract 0.001% 142 0.01% 148 0.1% 154 Untreated control 0% 100

As shown in Table 1, it can be seen that the Dragon Blood resin extract used as an active ingredient in the present invention has an excellent effect of promoting the growth of keratinocytes. In addition, the growth of keratinocytes proceeded more rapidly than the equivalent of the centellar extract having excellent effect of promoting keratinocyte growth, and the effect of promoting the division of keratinocytes increased proportionally as the concentration of components increased. .

Test Example 2 Active Oxygen Scavenging Effect

In order to investigate the inhibitory effect of the generation of active oxygen generated by ultraviolet light of the dragon blood resin extract, that is, the scavenging effect of the active oxygen, an experiment was performed as follows using a fluorescent material. For the comparison of free radical scavenging effect, the Vaccinium Angustifolium (Blueberry) Fruit Extract, which is widely known to inhibit free radical production, was compared and tested.

The blueberry extract used in this experiment was purchased from Bioland (Bioland, South Korea).

First, the cell line used in the experiment was a human keratinocytes HaCaT cell line distributed from Dr. Fusenig of the German Cancer Research Center, which was a 96-well black plate for fluorescence measurement. Aliquots at 2.0 × 10 ⁴ per well, and 37 ° C., 5% CO ₂ conditions using DMEM (Dulbeccos Modification of Eagles Medium, FBS 10%, Gibco, USA) medium with penicillin / streptomycin After incubation for 1 day at 0.5, 0.25, 0.125, 0.0625, 0.03125% of Dragon Blood resin extract or blueberry extract, respectively The concentration was treated.

Next, the test sample was added and incubated for 24 hours, and then washed with HCSS (HEPES-buffered control salt solution, Gibco, USA) to remove the remaining medium, and DCFH-DA (2 ', 7'-dichlorodihydro) prepared at 20 μM in HCSS. -fluorescein diacetate, Molecular Probes, USA) was added 100μl and incubated for 20 minutes at 37 ℃, 5% CO ₂ conditions and washed with HCSS. After 100 μl of HCSS treated for each sample concentration, the fluorescence of DCF (dichlorofluorescein) oxidized with active oxygen was measured with a fluorescence plate reader (Ex = 485 nm, Em = 530 nm). After UVB (30mJ / cm ² ) was irradiated and measured immediately after the treatment (Table 2) and 3 hours after the treatment (Table 3) by the fluorescent plate reader (Ex = 485nm, Em = 530nm). At this time, the control group was used in the untreated group of the dragon blood resin extract and blueberry extract.

Fluorescence immediately after treatment density(%) Fluorescence Dragon Blood Resin Extract 0.03125% 87.55 0.0625% 85.06 0.125% 83.74 0.25% 81.53 0.5% 79.45 Blueberry Extract 0.03125% 91.33 0.0625% 89.07 0.125% 87.89 0.25% 85.64 0.5% 83.28 Untreated control 0% 100

Fluorescence after 3 hours of treatment density(%) Fluorescence Dragon Blood Resin Extract 0.03125% 77.23 0.0625% 75.55 0.125% 73.97 0.25% 71.75 0.5% 68.81 Blueberry Extract 0.03125% 82.74 0.0625% 79.39 0.125% 76.85 0.25% 73.53 0.5% 71.92 Untreated control 0% 100

As shown in Tables 2 and 3, it can be seen that the dragon blood resin extract used as an active ingredient in the present invention has an excellent effect of inhibiting the generation of active oxygen. In addition, as compared with the blueberry extract excellent in the inhibitory effect of the production of active oxygen showed an equivalent or more efficacy, as the concentration of the component increased the inhibitory effect of the active oxygen production proportionally increased.

Test Example 3 Free Radical Scavenging Effect

In order to determine the free radical scavenging effect of the Dragon Blood resin extract was tested by the following method. The effect was confirmed by comparing with the blueberry extract in the same manner as in Example 2.

As a measuring method, 1,1-diphenyl-2-pyrylylhydrazyl (1,1-Diphenyl-2-pycryl-Hydrazyl; DPPH) method was used, and 1,1-diphenyl-2-picryl The 2-hydrazyl reagent was purchased from Sigma, USA (St. Louis, MO, USA) and used. This reagent is a formulation that is mainly used in in vitro methods, which are present as relatively stable free radicals and are primarily used to confirm free radical scavenging action.

The dragon blood resin extract or blueberry extract used for DPPH measurement was placed in 96-well plates at concentrations of 5.0, 1.0, 0.1 and 0.01%, respectively, and the total volume of DPPH prepared with 5 mM ethanol solution was 200 μl. After standing at 30 ° C., absorbance was observed using a 520 nm ELISA reader. The free radical scavenging action (%) was calculated by the following Equation 1, and the results are shown in Table 4 below.

A: Absorbance of control wells without sample

B: Absorbance of Experimental Wells Treated with Samples

density(%) Free radical scavenging effect (%) Dragon Blood Resin Extract 0.01% 35.2 0.1% 52.3 One% 78.8 5% 90.1 Blueberry Extract 0.01% 25.7 0.1% 48.5 One% 63.1 5% 79.0 Untreated control 0% 0

As shown in the results of Table 4, it can be seen that the dragon blood resin extract used as an active ingredient in the present invention has an excellent effect of eliminating free radicals. In addition, free radical scavenging effect was equal to or higher than that of known blueberry extract, and as the concentration of the component increased, the free radical scavenging effect increased proportionally.

Test Example 4 Increasing Antioxidant Protective Activity of Human Cell Lines

Dragon Blood resin extract was tested by the following method to determine the effect of increasing antioxidant defenses in human cell lines. The effect was confirmed by comparison with the blueberry extract in the same manner as in Example 2.

The cell lines used in this experiment were human keratinocyte HaCaT cell lines divided into 6.0 × 10 ⁶ cells in 150 × 150 culture plates and incubated for 1 day, followed by 5.0%, 1.0% and 0.1% concentrations of Dragon Blood resin extract or blueberry extract, respectively. The test sample was treated with. DMEM (Dulbeco's modified eagles medium, GIBCO BRL, Life Technologues) medium containing 10% fetal bovine serum (FBS) used for the culture was used and cultured at 37 ℃, 5% CO ₂ conditions. 1 × PBS (phosphate buffered saline, Sigma Co.) was used for washing after incubation. After the test sample was added and cultured for 1 day, UVB was treated at an intensity of 30mJ / cm ² , and cultured for 1 day at 37 ° C. and 5% CO ₂ .

Antioxidant defense factors to be measured are superoxide dismutase (SOD), catalase (catalase) and glutathione (GSH). 1969; 244: 6049-6055), catalase was measured according to Budhuin's method (Biochem. J. 1964; 92: 179-184), and glutathione according to Akerboom's method (Methods in Enzymology 1981; 77: 373-382). .

The antioxidant protective factor recovery effect was expressed in% of the activity compared to this value in terms of 100% of the control (Control) not irradiated with UVB, the results are shown in Table 5 below.

density% activation(%) SOD Catalase GSH Control group 100 100 100 UVB treatment group 42.8 65.3 60.7 Dragon Blood
Resin extract 0.1% 51.3 64.7 63.1 One% 63.7 70.2 78.6 5% 75.3 78.5 89.9 Blueberry Extract 0.1% 45.6 61.9 59.4 One% 58.7 66.3 75.5 5% 70.6 74.9 85.3

As shown in the results of Table 5, it can be seen that the dragon blood resin extract used as an active ingredient in the present invention is excellent in antioxidant efficacy by restoring the activity of antioxidant defenses reduced by ultraviolet rays. In addition, compared with the blueberry extract, the effect of restoring the activity of the antioxidant defenses was superior to the equivalent or more, and as the concentration of the component increased the antioxidant effect increased proportionally.

Test Example 5 MMP-1 and Procollagen Analysis

MMP-1 and procollagen analyzes were performed in the following manner to investigate the effects of the Dragon Blood resin extract on reducing the biosynthesis of MMP-1 and promoting the biosynthesis of type 1 procollagen. In this experiment, adenosine, a well-known anti-wrinkle functional component of MMP-1 biosynthesis and the type 1 procollagen-promoting effect, was compared and tested.

Adenosine used in this experiment was purchased from ATLAB (South Korea).

First, the fat layer of the normal epidermis is removed and chopped to separate the epidermis and the dermis with collagenase, and then the epidermis and the dermis are put into 0.25% trypsin solution, respectively, at 37 ° C. and 5% CO ₂ incubator. Treated for a minute. Since vortex was performed to release the keratinocytes and fibroblasts, respectively. After collecting and washing the free cells, keratinocytes were cultured at 1 × 10 ⁴ cells / cm ² in KGM (keratinocyte growth medium, Clonetics, USA), and fibroblasts were added with 10% fetal bovine serum (FBS). Cultured in DMEM medium. When 70 ~ 80% of the cells were grown, the cells were subcultured at a ratio of 1: 3 and the cells cultured in the third to fourth passages were used for the experiment.

For experiments to measure the amount of MMP-1, fibroblasts were cultured in at least 90% in 48 well plates and then starved for one day. Thereafter, the plate was washed twice with PBS, and 100 쨉 l of PBS was added. The plate was opened with a UV light A (Dermlight cube 401 equipped with a UVA filter, UVAtec, USA) at 15 J / cm ² . Immediately after irradiation, the cells were washed once with PBS and treated with Dragon Blood resin extract and adenosine at 0.001 and 0.01% concentration in DMEM without fetal calf serum, and then incubated for 48 hours at 37 ° C. and 5% CO ₂ incubator. The amount of free MMP-1 in the medium was measured using the MMP-1 human ELISA system (RPN2610, Amersham Pharmacia Biotech, UK) and corrected for the total protein amount of fibroblasts (Table 6). . At this time, the control group was used as the untreated group of dragon blood resin extract and adenosine.

Meanwhile, in order to measure the amount of procollagen, 0.001 and 0.01% of dragon blood resin extract and adenosine were added to the starvation state without UV irradiation under the same conditions as the method for measuring the amount of MMP-1, and for 24 hours. The amount of procollagen released in the medium was then measured using the procollagen type-1 C-peptide EIA kit (MK101, Takara, Japan) (Table 7). At this time, the control group was used as the untreated group of dragon blood resin extract and adenosine.

density(%) Free MMP-1 Volume Dragon Blood Resin Extract 0.001% 75 0.01% 68 Adenosine 0.001% 77 0.01% 67 Untreated control 0% 100

density(%) Free Procollagen Volume Dragon Blood Resin Extract 0.001% 109 0.01% 123 Adenosine 0.001% 108 0.01% 124 Untreated control 0% 100

As shown in the results of Table 6, it can be seen that the Dragon Blood resin extract used as an active ingredient in the present invention inhibits MMP-1. In addition, when compared with adenosine, the effect was equally equivalent, and as the concentration of the component increased, the amount of MMP-1 decreased proportionally.

In addition, as shown in the results of Table 7, it can be seen that the dragon blood resin extract used as an active ingredient in the present invention increases the biosynthesis of type 1 procollagen. In addition, when compared with adenosine, the same level of effect was observed, and as the component concentration increased, the amount of procollagen produced increased proportionally.

Reference Example 1 Preparation of Example 1 and Comparative Examples 1 and 2

To the cosmetic composition of the cream formulation of Example 1 and Comparative Examples 1-2 with the composition of Table 8.

Component (content:% by weight) Example 1 Comparative Example 1 Comparative Example 2 1.Beads wax 2 2 2 2. Stearyl alcohol 3 3 3 3. Stearic acid 8 8 8 4. Squalene 10 10 10 5.propylene glycol monostearate 3 3 3 6. Polyoxyethylene ether One One One 7. Preservative Suitable amount Suitable amount Suitable amount 8. Glycerin 5 5 5 9. Propylene Glycol 4 4 4 10. Triethylamine One One One 11. Sodium Polyacrylate 0.2 0.2 0.2 12. Purified Water To 100 To 100 To 100 13. Dragon Blood Resin Extract 0.1 - - 14. Adenosine - - 0.1 15. Carbomer Suitable amount Suitable amount Suitable amount

<Manufacturing Method>

1) The components 1-7 were melt | dissolved by heating at 65-75 degreeC, and the oil phase part was manufactured.

2) Components 8-12 were dissolved by heating at 65-75 ° C., thereby preparing an aqueous part.

3) While stirring 2), 1) and component 15 were slowly added and emulsified at 7000-8000 rpm for 5 minutes to prepare a cream formulation.

4) After cooling to 45 ℃ was prepared by adding components 13-14 and dispersed for 1 minute at 1500rpm.

[Test Example 6] Wrinkle improvement effect

In order to examine the wrinkle improvement effects of Examples 1 and Comparative Examples 1 and 2, 60 women in their 30s were divided into 3 sets of 20 people each, and Example 1 and Comparative Example 1 were applied to the designated eye area for 2 weeks every morning and evening. After applying the cream of ~ 2 each, a replica of silicon was made, and the condition of wrinkles at the designated area was measured by a visometer (SVI60, Courage + Khazaka electronic GmbH, Germany), an image analyzer. The results are shown in Table 9 below. This result represents the average of each parameter value after 8 weeks minus the parameter value 8 weeks ago. In other words, the negative the value, the higher the wrinkle improvement effect.

R1 R2 R3 R4 R5 Example 1 -0.24 -0.20 -0.13 -0.08 -0.08 Comparative Example 1 0.27 0.25 0.21 0.04 0.03 Comparative Example 2 -0.25 -0.20 -0.12 -0.09 -0.08

R1: difference between the highest and lowest of the wrinkle contour

R2: The wrinkle contour line is arbitrarily divided into 5 squares, and the average of R1 values

R3: Highest value of R1 divided by 5

R4: Baseline of the wrinkle contour, minus the top and valley of each angle

R5: Average of the baseline of the wrinkle contour, minus each wrinkle contour

As can be seen in Table 9, Example 1 containing the dragon blood resin extract compared to Comparative Example 1 showed a large negative value in all items. In addition, even when compared with Comparative Example 2 containing adenosine, a wrinkle improving functional ingredient, negative values of the same level were shown. Therefore, it can be seen that the skin wrinkle improvement effect of the cosmetic composition of the present invention is very excellent.

Test Example 7 Skin Barrier Function Restoration Effect

In order to measure the skin barrier function recovery effect of Example 1, after repeatedly applying acetone to the skin of a hairless mouse to damage the barrier function, epidermal moisture loss (TEWL, transdermal water loss) servomed company (Sweden) When it reaches 4.0 g / m 2 / h as measured by an evaporimeter EP1, Example 1 and Comparative Example 1 were applied to an area of 5 cm 2, and after 1, 2, 4 and 8 hours, the amount of epidermal moisture was lost. The extent to which barrier function is restored is assessed by assessing the extent to which measurements are reduced. At this time, a conventional lipid mixture (2: 1: 1 mixture of ceramide: cholesterol: fatty acid) was used as a positive control. The epidermal moisture loss in the injured barrier state was set to 100% and the change over time was compared, and the results are shown in Table 10 below.

sample Epidermal moisture loss After 1 hour After 2 hours After 4 hours After 8 hours Example 1 57 48 37 25 Comparative Example 1 87 79 76 73 Lipid mixture 65 45 38 24

As can be seen from the results of Table 10, Example 1 containing the dragon blood resin extract was less epidermal moisture loss than Comparative Example 1 does not contain the active ingredient, the skin barrier function recovery better than the lipid mixture of the positive control Effect.

Test Example 8 Moisturizing Effect Test in Human Body

To complain of dryness, or to divide the adult 40 men and women, which are considered to be dry skin, into two sets, each group was provided with Example 1 and Comparative Example 1 and applied to the face twice daily for four weeks. Moisturizing power was compared by measuring TEWL at constant temperature and humidity conditions (24 ° C., 40% relative humidity) at the time of 2 weeks and 4 weeks after the start of coating and after application, and the results are shown in FIG. 1. In addition, in order to determine the moisturizing sustainability in one application, after applying Example 1, the skin moisture content was measured by a corneometer (corneometer) for 6 hours, and the results are shown in FIG. 2.

<How to measure Trans Epidermal Water Loss (TEWL)>

Principle: The amount of water evaporating from the skin is calculated according to the area and time, and the amount of water evaporating is read by the temperature and humidity electronic sensor to measure the moisture of the skin.

1. Environmental conditions: Keep the temperature and humidity constant, and keep the minimum number of people except the measuring person and the subject, and cut off the air flow if possible.

2. Place the TEWL probe on the subject's measurement site.

3. Do not apply excessive force to the measuring part. Place the probe lightly so as not to leave marks on the skin and make an angle of 90 degrees to the ground.

4. Measure the value of the skin's TEWL level over time (1 ~ 3 minutes).

5. Be careful not to let the temperature of the measurer pass by holding the handle of the probe as far away as possible.

6. Calculate the amount of water (g / m ² / h) that evaporates per hour per area.

As can be seen from the results of FIG. 1, when Example 1 containing the dragon blood resin extract was applied to the skin, the TEWL change value (water evaporation amount) decreased over time. Therefore, it turned out that the moisturizing effect of the cosmetic composition of this invention is excellent.

<How to measure the Koniometer>

Principle: Moisture is measured by measuring the amount of moisture in the skin's epidermis using a sensor and quantifying the amount of moisture.

1. Place a corneometer probe on the skin area to be measured.

2. When the probe is pressed on the skin, the electrical capacitance of the skin is quantified through the sensor and displayed on the screen.

3. Repeat the measurement with different measuring area.

4. After measuring once, wipe the sensor with Kimwipe and measure again.

As can be seen from the results of Figure 2, Example 1 containing the dragon blood resin extract showed a greater increase in the amount of moisture in the skin over time than the non-treated group, thereby providing an excellent moisturizing effect of Example 1 You can check it.

Test Example 9 Usability Evaluation

Twenty-four adult men and women who thought of dryness or dry skin were divided into two sets, and each group was provided with Example 1 and Comparative Example 1 to be applied to the face twice daily for four weeks.

After using for 4 weeks, the evaluation of the skin dryness improvement effect was conducted through a questionnaire about usability, and the results are shown in Table 11 below.

Applicator Number of Respondents Very good Good usually Inadequate Example 1 6 5 One 0 Comparative Example 1 0 One 7 4

As can be seen from the results of Table 11, the person who applied Example 1 was found to feel better than the person who applied Comparative Example 1, the skin dryness improvement effect and moisturizing effect.

Test Example 10 Whitening Effect on Human Skin

In order to determine the whitening effect of the cosmetics prepared in Example 1 and Comparative Example 1 was carried out the following experiment.

First, the opaque tape with a hole of 1.5 cm diameter was attached to the upper forearm of 14 healthy men, and then 1.5 ~ 2 times of UVB (Minimal Erythema Dose) of each subject. ) To induce blackening of the skin, and the whitening cosmetics prepared in Example 1 and Comparative Example 1 were applied twice a day (morning, evening) for two months every day, and after two months the chromameter (Chromameter, Minolta CR-300) was used to measure the contrast of the skin.

The determination of the effect was determined by obtaining a "L" value representing the contrast of the skin. For reference, Korean skin color that is not artificially burned generally has a value of 50 to 70. The effect was determined by measuring the degree of black and white of the skin using a color difference meter (Minolta CR2002). L * a * b * colorimetric system is used for displaying the color, but in the present invention, the L * value (brightness) is mainly used as an index. The L * value was calibrated with a standard whiteboard, and the measurement was performed to measure the pigmentation portion evenly by repeating the measurement five times or more at one site. The difference in skin color (ΔL *) between the start point of application (before application) and the completion point (after two months of application) was calculated according to Equation 2 below, and the results are shown in Table 12 below. The whitening effect is determined by the comparison of ΔL * between the sample application site and the control site. The higher the ΔL * value, the better the whitening effect.

Evaluation item Example 1 Comparative Example 1 Whitening effect (ΔL) 2.93 0.81

In the results of Table 12, the ΔL value of Example 1 containing the dragon blood resin extract was significantly higher than the comparative example 1 containing no dragon blood resin extract, through which the present invention It can be seen that the composition containing the dragon blood resin extract provides an excellent whitening effect.

[Test Example 11] Confirmation test for promoting skin turnover in human skin

Example 1 and Comparative Example 1, control group (untreated group) and positive control group (1% lactic acid treated group) to investigate the skin turnover (Turn over) promoting effect, 28 healthy men and women (mean age) 34 years old), before applying the test substance, measured the color inside the upper arm of the test subject with a colorimeter, and artificially tanned it with a cream containing dihydroxy acetone (DHA). Was compared. After applying twice a day 0.2% by weight of 1% lactic acid dissolved in the mixed solvent (water: ethanol: butylene glycol = 80: 15: 5) used in the compositions and positive control of Example 1 and Comparative Example 1 While measuring the degree of discoloration every day with a colorimeter, the time taken to return to the original skin color (chromatic change due to protein discoloration = skin turnover time) was measured. Skin turnover acceleration was calculated by the following Equation 3, and the results are shown in Table 13 below.

The turnover time is the time taken for the existing stratum corneum to be replaced with a new stratum corneum, that is, the time taken for the skin to return to normal after being tanned by DHA.

Test substance Turnover time (day) Turnover Promotion Rate (%) Control group 18.5 - Positive control group 12.5 32.4 Example 1 12.0 35.1 Comparative Example 1 15.5 16.2

In the results of Table 13, the whitening cosmetic of Example 1 according to the present invention promotes turnover than Comparative Example 1 and the control, and also showed that the turnover period is shorter than the positive control, Example 1 according to the present invention It can be seen that effectively promotes melanin release.

Hereinafter, the formulation examples of the composition will be described, but this is not intended to limit the invention but only to explain in detail.

[Formulation Example 1] Suspension emulsion lotion

To the suspension in the composition of Table 14 can be prepared suspension lotion.

Ingredient Content (% by weight) Methylparaben
Spices
Cetyloctanoate
Polyoxyethylene Cured Castor Oil
Polysiloxane
Tocopherol Acetate
ethanol
glycerin
Propylene glycol
Carboxyvinyl polymer
Dragon Blood Resin Extract
Purified water Suitable amount
Suitable amount
1.0
0.6
0.4
0.1
12.0
6.0
2.0
0.12
0.1
TO 100

Formulation Example 2 Essence

Essence may be prepared by the composition of Table 15 below.

Ingredient Content (% by weight) Methylparaben
Spices
Cetyloctanoate
Octyldodes-16
Coles-24 / Setes-24
Polysiloxane
Tocopherol Acetate
ethanol
glycerin
Xanthan Gum
Propylene glycol
Carboxyvinyl polymer
Dragon Blood Resin Extract
Purified water Suitable amount
Suitable amount
1.0
0.5
0.2
0.4
0.1
6.0
15.0
0.07
4.0
0.12
0.1
TO 100

[Formulation Example 3] Nourishing cream

To the nutrition cream was prepared in the composition of Table 16 below.

Ingredient Content (% by weight) Liquid paraffin
Cetostearyl alcohol
Wax
Polysiloxane
Lipophilic monostearic acid stearate
Stearic acid
Cetearylglucoside
Monostearic acid polyoxyethylene sorbitan (20 EO)
Hydrogenated lecithin
Cetyloctanoate
Methylparaben
Propylparaben
Spices
Sodium ethyleneamine tetraacetate
glycerin
Carboxyvinyl polymer
Propylene glycol
Dragon Blood Resin Extract
Purified water 15.0
2.0
3.0
0.5
2.0
2.0
1.5
1.3
2.0
1.0
0.2
0.1
Suitable amount
0.02
5.0
0.10
2.0
0.5
TO 100

Formulation Example 4 Gel

To prepare a gel in the composition of Table 17.

Ingredient Content (% by weight) antiseptic
Spices
Cetearylglucoside
Cetyloctanoate
glycerin
Jojoba wax
Dipantenol
ethanol
Carboxyvinyl polymer
Propylene glycol
Dragon Blood Resin Extract
Purified water Suitable amount
Suitable amount
1.5
1.0
5.0
3.0
1.0
7.0
0.6
3.0
0.5
TO 100

[Formulation Example 5] Pack

To prepare a pack in the composition of Table 18.

Ingredient Content (% by weight) Methylparaben
Spices
Cetyloctanoate
Polyoxyethylene Cured Castor Oil
Polysiloxane
Cellulose gum
ethanol
glycerin
Propylene glycol
Polyvinyl alcohol
Dragon Blood Resin Extract
Purified water Suitable amount
Suitable amount
1.0
0.6
0.2
0.2
6.0
5.0
3.0
15.0
1.0
TO 100

Claims (7)

A cosmetic composition comprising a dragon blood resin extract as an active ingredient. The cosmetic composition of claim 1, wherein the dragon blood resin extract is contained in an amount of 0.0001 to 20% by weight based on the total weight of the cosmetic composition. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for enhancing the moisturizing power. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for anti-aging. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for improving wrinkles. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for enhancing elasticity. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for whitening.

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