KR101915655B1 - Cosmetic composition containing Croton Lechleri resin extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a Dragon Blood resin extract. The cosmetic composition of the present invention is excellent in growth promoting effect of keratinocyte cells, active oxygen scavenging effect produced by ultraviolet rays, It promotes the biosynthesis of type 1 procollagen by promoting the biosynthesis of MMP-1 by ultraviolet rays and promotes the release of melanin generated thereby to provide excellent skin aging prevention and moisturizing effect and also has excellent antimicrobial and anti-inflammatory effect, It is also effective in reducing pores, reducing sebum, and making the turnover of horny smooth.

Description

Cosmetic composition containing Croton Lechleri resin extract containing Dragon Blood resin extract

The present invention relates to a cosmetic composition containing an extract of Dragon Blood resin as an active ingredient and capable of improving the overall skin condition.

Skin protects the human body from external physical and chemical stimuli, and is divided into three layers: epidermis, dermis, and subcutaneous fat tissue. In particular, skin contains about 65 ~ 70% of the human body's moisture, which transports various physiologically active substances necessary for the human body and helps the skin to maintain its moist condition. Skin also plays an important role in regulating the evaporation of this water in vitro.

The epidermis is divided into the stratum corneum, the granular layer, the polar layer, and the basal layer from the outside. The outer layer of the epidermis contains about 10 to 20% of water and is present in the outermost part of the human body, thereby forming a natural protective film made of sweat from sebum and sweat glands from the sebaceous glands to prevent water evaporation from the outside of the body, Blocks material over-penetration. The cells that make up this stratum corneum have a high concentration of Natural Moisturizing Factor (NMF), which is a water-soluble ingredient, to help the skin to maintain flexibility and maintain proper moisture. However, ultraviolet rays, excessive physical and chemical stimuli from the outside, stress and nutritional deficiency deteriorate the normal functions of the skin and promote skin aging phenomenon such as loss of elasticity, keratinization and wrinkle formation. In particular, if the keratinization process in which the keratinocytes are successively made in sequence and the outermost keratinocytes from the outermost layer are occasionally deviated, the epidermis keeps a constant thickness. If the keratinization process is not smooth, abnormal keratinocytes are formed, The skin becomes rough and dull, fine wrinkles are formed, and the skin ages in the end. In addition, the biosynthesis of type 1 procollagen through biosynthesis of MMP-1 by ultraviolet light related to the degradation of active oxygen and skin substrate produced by ultraviolet rays, and the degradation of fibroblast dividing ability in skin dermal layer are also related to skin aging .

U.S. Patent Application No. 2002-494567

Accordingly, the inventors of the present invention have made efforts to find a raw material having a skin condition improving effect without inducing skin side effects among naturally occurring substances. As a result, they found that Dragon Blood resin extract is very effective for improving overall skin condition and completed the present invention .

Accordingly, an object of the present invention is to provide a cosmetic composition which is effective for improving skin condition as a whole, including skin moisturizing, elasticity improvement, anti-aging, whitening effect, antibacterial, anti-inflammatory effect, pore reduction, sebum reduction, acne improvement, To provide a composition.

In order to achieve the above object, the present invention provides a cosmetic composition comprising Dragon Blood resin extract as an active ingredient.

The cosmetic composition of the present invention contains a Dragon Blood resin extract as an active ingredient to reduce the growth promoting effect of keratinocytes, the active oxygen scavenging effect generated by ultraviolet rays, and the biosynthesis of MMP-1 due to ultraviolet light related to skin substrate degradation The present invention can be effectively used as a cosmetic composition for anti-aging and skin moisturizing enhancement because it has an effect of promoting the biosynthesis of type 1 procollagen and a whitening effect by accelerating the release of melanin produced. In addition, it has excellent antimicrobial and anti-inflammatory effects, which can improve the symptoms of acne, reduce pores, reduce sebum, and smooth the turnover of horny skin.

1 is a graph showing the results of measurement of the moisture resistance of a composition according to the present invention by a method for measuring transdermal water loss (TEWL).
FIG. 2 is a graph showing the result of measurement of the amount of skin moisture by the composition according to the present invention with a coneometer.

The present invention provides a cosmetic composition comprising Dragon Blood resin extract as an active ingredient.

Hereinafter, the present invention will be described in more detail.

The Croton Lechleri used in the present invention is a giant 10-20 m native to Peru, which inhabits the upper South America region of the Amazon. Due to the red color of the resin, the resin of this tree, named Dragon's Blood, has been used as a traditional remedy since ancient times.

The extract of Croton Lechleri contains proanthocyanidin and taspine as active ingredients. Proanthocyanidins are a type of flavonoids that give off a flavor and produce colors in vegetables, fruits, and wines. They are known to be effective against cancer and have anti-cancer effects. TASPIN is an organic compound containing nitrogen, and it is known that it gives a good effect to skin such as wound healing.

The dragon-blood resin extract used in the present invention is preferably contained in an amount of 0.0001 to 20.0% by weight based on the total weight of the composition. When the content is less than 0.0001% by weight, the effect thereof is insignificant. When the content is more than 20.0% by weight, there arises a problem of feeling of use and stability of the formulation.

The dragon-blood resin extract used in the present invention can be produced by a conventional production method in the art, and the production method thereof is not particularly limited. For example, it can be obtained by a general method of leaching a dragon-blood resin into water or an organic solvent, purifying and separating. The organic solvent to be used is not particularly limited, and may be a C ₁ to C ₅ lower alcohol, ether, ethyl acetate or chloroform. The C ₁ to C ₅ lower alcohols may be used for mixing one or more kinds selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol .

The cosmetic composition according to the present invention is effective for promoting the growth of excellent keratinocytes, the active oxygen scavenging effect generated by ultraviolet rays, the effect of promoting type 1 procollagen biosynthesis through reduction of biosynthesis of MMP-1 due to ultraviolet rays related to skin substrate degradation, It exhibits excellent tyrosinase activity inhibiting effect and exhibits excellent skin aging prevention and moisturizing effect. Therefore, the composition of the present invention is useful as a composition for promoting keratinocyte cell growth, a composition for promoting skin antioxidation, a composition for promoting biodegradation of integrated type 1 procollagen, a composition for skin whitening enhancement, And can be usefully used in cosmetics as a moisturizing and improving composition.

Further, the cosmetic composition according to the present invention is excellent in anti-inflammatory and antibacterial effects, and is also effective in improving acne. In addition, the pores can be reduced, the sebum can be reduced, and the turnover of horny skin can be smoothly performed, thereby making it possible to effectively use it to trim skin texture.

The cosmetic composition according to the present invention may contain other ingredients which can give a synergistic effect to the main effect within the range not impairing the main effect of the present invention, Other ingredients besides the active ingredient may be mixed and selected without difficulty by those skilled in the art depending on the formulation of the other cosmetics or the purpose of use.

For example, the cosmetic composition may contain a skin absorption promoting substance to increase its effect. In addition, the cosmetic composition of the present invention may comprise a material selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts. In addition, the cosmetic composition of the present invention may contain other essential ingredients, if necessary, in combination with other cosmetic ingredients. Examples of such ingredients include moisturizers, emollients, surfactants, organic and inorganic pigments , An organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, an alcohol, a coloring matter, a flavor, a blood circulation promoter, a cold agent, an antiperspirant agent and purified water.

The other ingredients to be contained in the cosmetic composition of the present invention are not limited to these, and the amount of the above ingredients may be adjusted within a range that does not impair the objects and effects of the present invention.

The cosmetic composition according to the present invention is not particularly limited in its formulation, and examples thereof include softening lotion, convergent lotion, nutritional lotion, nutritional cream, massage cream, eye cream, eye essence, essence, cleansing cream, cleansing lotion, cleansing foam Such as cleansing water, packs, powders, makeup bases, foundation, lipstick, body lotion, body cream, body oil, body essence, body cleanser, serum, soap, milky lotion, lotion, ointment, gel, cream, patch, .

Hereinafter, the present invention will be described in more detail by way of Examples and Test Examples, but the present invention is not limited thereto.

[Test Example 1] Effect of promoting keratinocyte cell growth - MTT assay

The effect of Dragon Blood resin extract on the growth of keratinocytes was measured by MTT method. In order to compare the effect of keratinocyte on the growth of keratinocytes, the growth rate of keratinocytes was measured and compared with Centella asiatica Extract, which is known to promote keratinocyte growth.

The Dragon Blood resin extract was purchased from Naturax (USA) and the centilla extract was purchased from Bioland (Korea).

First, keratinocytes were cultured in a 96-well plate at a concentration of 5 × 10 ³ per well per well and cultured for 24 hours. Then, the extracts of the dragon-blood resin and the centilla callus were treated with 0.001, 0.01 and 0.1% After incubation for 48 hours, the culture medium was removed by inhalation, washed once with PBS, and 100 μl of a 0.5 mg / ml solution of MTT (Methylthiazolyldiphenyl-tetrazolium bromide, Sigma, USA) for 37 ℃, and cultured at 5% CO _2. Thereafter, the culture solution was inhaled and removed, and then 200 μl of a dimethyl sulfoxide (DMSO) solution was added. After shaking for 10 minutes in a shaker, the absorbance at 540 nm was read in an ELISA reader (DIbiotech, Korea) . As a control group, Dragon Blood resin extract and no-treatment group of Centella asiatica extract were used, and the results are shown in Table 1 below.

density(%) Absorbance Dragon Blood Resin Extract 0.001% 153 0.01% 163 0.1% 170 Centipede extract 0.001% 142 0.01% 148 0.1% 154 Untreated control group 0% 100

As shown in Table 1 above, it can be seen that the Dragon Blood resin extract used as an active ingredient in the present invention is excellent in promoting keratinocyte cell growth. In addition, the keratinocyte growth progressed more rapidly than that of the Centella asiatica extract, which has the effect of promoting keratinocyte cell growth, and the effect of accelerating the division of keratinocytes increased proportionally as the component concentration was increased .

[Test Example 2] Effect of active oxygen scavenging

In order to investigate the effect of inhibiting the formation of active oxygen generated by ultraviolet rays of Dragon Blood resin extract, that is, the effect of scavenging reactive oxygen, the following experiment was performed using a fluorescent substance. In order to compare the effects of free radical scavenging, blueberry extract (Vaccinium Angustifolium (Blueberry) Fruit Extract), which is known to inhibit active oxygen production, was tested and compared.

The blueberry extract used in this experiment was purchased from Bioland (Korea).

First, the cell line used for the experiment was a human keratinocyte HaCaT cell line (human keratinocyte HaCaT cell line), which was purchased from Dr. Fusenig of the German Cancer Research Center, and was cultured in a 96 well black plate for fluorescence measurement, the per well 2.0 × 10 ⁴ pieces dispensed, penicillin / streptomycin (penicillin / streptomycin) DMEM with the addition of the 37 ℃ using (Dulbeccos Modification of Eagles medium, FBS 10%, Gibco, USA) medium, 5% CO ₂ conditions After 1 day of incubation, the dragon-blood resin extract or blueberry extract was added at 0.5, 0.25, 0.125, 0.0625, 0.03125% Lt; / RTI >

Then, the test sample was added and incubated for 24 hours. Then, the remaining medium was removed by washing with HCSS (HEPES-buffered control solution, Gibco, USA), and DCFH-DA (2 ', 7'-dichlorodihydro -fluorescein diacetate, Molecular Probes, USA) was added, and the cells were incubated at 37 ° C and 5% CO ₂ for 20 minutes and washed with HCSS. The fluorescence of DCF (dichlorofluorescein) initially oxidized to active oxygen was measured with a fluorescent plate reader (Ex = 485 nm, Em = 530 nm) after adding 100 μl of treated HCSS to each sample concentration. The fluorescence of the sample was measured with a fluorescent plate reader (Ex = 485 nm, Em = 530 nm) after irradiation of UVB (30 mJ / cm ² ) and immediately after treatment (Table 2) and after 3 hours of treatment (Table 3). At this time, the control group was a group of no treatment of Dragon Blood resin extract and blueberry extract.

Fluorescence immediately after treatment density(%) Fluorescence Dragon Blood Resin Extract 0.03125% 87.55 0.0625% 85.06 0.125% 83.74 0.25% 81.53 0.5% 79.45 Blueberry extract 0.03125% 91.33 0.0625% 89.07 0.125% 87.89 0.25% 85.64 0.5% 83.28 Untreated control group 0% 100

Fluorescence after 3 hours of treatment density(%) Fluorescence Dragon Blood Resin Extract 0.03125% 77.23 0.0625% 75.55 0.125% 73.97 0.25% 71.75 0.5% 68.81 Blueberry extract 0.03125% 82.74 0.0625% 79.39 0.125% 76.85 0.25% 73.53 0.5% 71.92 Untreated control group 0% 100

As shown in Tables 2 and 3, the dragon-blood resin extract used as an active ingredient in the present invention is excellent in inhibiting the production of active oxygen. In addition, it showed the same effect as the blueberry extract, which has excellent inhibitory effect on the production of active oxygen, and the inhibitory effect on the production of active oxygen increased proportionally as the concentration of the component increased.

[Test Example 3] Free radical scavenging effect

The free radical scavenging effect of Dragon Blood resin extract was examined by the following method. The effect was confirmed in comparison with the blueberry extract in the same manner as in Test Example 2.

Diphenyl-2-pycryl-hydrazyl (DPPH) method was used as the measurement method, and 1,1-diphenyl-2-picryl -2-hydrazine reagent was purchased from Sigma (St. Louis, MO, USA). This reagent is a relatively stable free radical and is used primarily in in vitro methods that are used primarily to identify free radical scavenging activity.

The above-mentioned Dragon Blood resin extract or blueberry extract used in the DPPH measurement was added to a 96-well plate at a concentration of 5.0, 1.0, 0.1 and 0.01%, respectively, and DPPH prepared in a 5 mM ethanol solution was added to a total volume of 200 After incubation for 30 minutes at < RTI ID = 0.0 > 0 C, < / RTI > The free radical scavenging activity (%) was calculated by the following equation (1), and the results are shown in Table 4 below.

A: Absorbance of control well without sample treatment

B: absorbance of the test well treated with the sample

density(%) Free radical scavenging activity (%) Dragon Blood Resin Extract 0.01% 35.2 0.1% 52.3 One% 78.8 5% 90.1 Blueberry extract 0.01% 25.7 0.1% 48.5 One% 63.1 5% 79.0 Untreated control group 0% 0

As shown in the results of Table 4, it can be seen that the Dragon Blood resin extract used as an active ingredient in the present invention is excellent in the effect of eliminating free radicals. In addition, the free radical scavenging effect was equivalent to that of the known blueberry extract, and the free radical scavenging effect was increased proportionally as the component concentration was increased.

[Test Example 4] Effect of increasing antioxidant defense activity of human cell lines

In order to investigate the effect of the dragonblood resin extract on the activity of antioxidant defense agents in human cell lines, the following test was conducted. The effect was confirmed in comparison with the blueberry extract in the same manner as in Test Example 2.

The cell line used in this experiment was divided into 6.0 × 10 ⁶ cells on a 150 × 150 culture plate with human keratinocyte HaCaT cell line and cultured for 1 day. Then, the dragon-blood resin extract or blueberry extract was added to the cells at 5.0%, 1.0% and 0.1% To the test sample. The cells were cultured in DMEM (Dulbecco's modified eagles medium, GIBCO BRL, Life Technologues) medium containing 10% fetal bovine serum (FBS) at 37 ° C and 5% CO ₂ . 1 × PBS (phosphate buffered saline, Sigma Co.) was used for washing after incubation. The test samples were incubated for 1 day, UVB was treated with 30 mJ / cm ² intensity, incubated at 37 ° C and 5% CO ₂ for 1 day, harvested and used for antioxidant defense factors.

Superoxide dismutase (SOD), catalase and glutathione (GSH) are the antioxidative defense factors to be measured. Superoxide dismutase is measured by the method of McCord (J. Biol. Chem 1949; 2449: 6049-6055), catalase was determined by the method of Budhuin (Biochem. J. 1964; 92: 179-184), glutathione was measured by the method of Akerboom (Methods in Enzymology 1981; 77: 373-382) .

The antioxidant defensive factor recovery effect was expressed as% activity in% of the control (control) without UVB irradiation, in terms of the activity compared with this value, and the results are shown in Table 5 below.

density% activation(%) SOD Catalase GSH Control group 100 100 100 UVB treatment group 42.8 65.3 60.7 Dragon Blood
Resin extract 0.1% 51.3 64.7 63.1 One% 63.7 70.2 78.6 5% 75.3 78.5 89.9 Blueberry extract 0.1% 45.6 61.9 59.4 One% 58.7 66.3 75.5 5% 70.6 74.9 85.3

As shown in the results of Table 5, it can be seen that the Dragon Blood resin extract used as an active ingredient in the present invention is excellent in antioxidant efficacy by restoring the activity of the antioxidant defense factor reduced by ultraviolet rays. In addition, the antioxidative effect of antioxidative defense factor was better than that of blueberry extract.

[Test Example 5] MMP-1 and procollagen analysis

MMP-1 and pro-collagen analysis were performed as follows in order to examine the effect of the dragonblood resin extract on reducing the biosynthesis of MMP-1 and promoting the biosynthesis of type 1 procollagen. In this experiment, adenosine (Adenosine), which is widely known as a wrinkle-reducing functional ingredient, has been shown to be effective in reducing MMP-1 biosynthesis and promoting the biosynthesis of type 1 procollagen.

The adenosine used in this experiment was purchased from Ethyl Lab (ATLAB, Korea).

After removing epidermis and dermis by collagenase, the epidermis and dermis were placed in 0.25% trypsin solution and incubated at 37 ° C in a 5% CO ₂ incubator for 10 Respectively. Then vortex was performed to release keratinocytes and fibroblasts, respectively. The cells were collected and washed, and then keratinocyte growth medium (Clonetics, USA) was cultured at a concentration of 1 × 10 ⁴ cells / cm ^{2. The} fibroblasts were cultured in the presence of 10% fetal bovine serum (FBS) And cultured in DMEM medium. When 70 ~ 80% of the cells were grown, the cells were subcultured at a ratio of 1: 3 and the cells cultured in the third to fourth passages were used for the experiment.

For the measurement of the amount of MMP-1, fibroblasts were cultured in a 48-well plate at a concentration of 90% or more and starvated for one day. Thereafter, the plate was washed twice with PBS, and 100 쨉 l of PBS was added. The plate was opened with a UV light A (Dermlight cube 401 equipped with a UVA filter, UVAtec, USA) at 15 J / cm ² . Immediately after the irradiation, the cells were washed once with PBS and treated with 0.001 and 0.01% of the dragon-blood resin extract and adenosine in DMEM without fetal bovine serum, respectively, and cultured in a 5% CO ₂ incubator at 37 ° C. for 48 hours The amount of MMP-1 liberated in the medium was measured using an MMP-1 human ELISA system (RPN2610, Amersham Pharmacia Biotech, UK) and corrected for the total protein amount of fibroblasts (Table 6) . At this time, Dragon Blood resin extract and no treatment group of adenosine were used as a control group.

On the other hand, in order to measure the amount of procollagen, 0.001 and 0.01% of a dragonblood resin extract and adenosine were added to the starvation state under the same conditions as those for measuring the amount of MMP-1, The amount of procollagen liberated in the medium was measured using a procollagen type-1 C-peptide EIA kit (MK101, Takara, Japan) (Table 7). At this time, Dragon Blood resin extract and no treatment group of adenosine were used as a control group.

density(%) The liberated amount of MMP-1 Dragon Blood Resin Extract 0.001% 75 0.01% 68 Adenosine 0.001% 77 0.01% 67 Untreated control group 0% 100

density(%) The amount of free procollagen Dragon Blood Resin Extract 0.001% 109 0.01% 123 Adenosine 0.001% 108 0.01% 124 Untreated control group 0% 100

As shown in the results of Table 6, it can be seen that the dragon-blood resin extract used as an active ingredient in the present invention inhibits MMP-1. In addition, when compared with adenosine, the same effect was exhibited, and the amount of MMP-1 decreased proportionally as the concentration of the component increased.

In addition, as shown in the results of Table 7, it can be seen that the dragonblood resin extract used as an active ingredient in the present invention increases the biosynthesis of type 1 procollagen. In addition, it showed the same level of effect as compared to adenosine, and the amount of procollagen produced increased proportionally as the concentration of the component increased.

[Referential Example 1] Preparation of Example 1 and Comparative Examples 1 and 2

The cream formulations of Example 1 and Comparative Examples 1 and 2 were prepared with the compositions shown in Table 8 below.

Component (content:% by weight) Example 1 Comparative Example 1 Comparative Example 2 1. Beads wax 2 2 2 2. Stearyl alcohol 3 3 3 3. Stearic acid 8 8 8 4. Squalene 10 10 10 5. Propylene glycol monostearate 3 3 3 6. Polyoxyethylene ether One One One 7. Preservatives Suitable amount Suitable amount Suitable amount 8. Glycerine 5 5 5 9. Propylene glycol 4 4 4 10. Triethylamine One One One 11. Small Dip Polyacrylate 0.2 0.2 0.2 12. Purified water To 100 To 100 To 100 13. Dragon Blood Resin Extract 0.1 - - 14. Adenosine - - 0.1 15. Carbomer Suitable amount Suitable amount Suitable amount

<Manufacturing Method>

1) Components 1 to 7 were dissolved by heating at 65 to 75 ° C to prepare an oil phase part.

2) Components 8 to 12 were dissolved by heating at 65 to 75 캜 to prepare a water-based part.

3) The ingredients 1) and 15 were gradually added while stirring the above 2) and emulsified at 7000-8000 rpm for 5 minutes to prepare a cream formulation.

4) After cooling to 45 ° C, components 13 to 14 were added and dispersed at 1500 rpm for 1 minute.

[Test Example 6] Wrinkle improving effect

In order to examine the wrinkle-improving effect of Example 1 and Comparative Examples 1 and 2, 60 women in their 30s were divided into three groups each consisting of 20 individuals, and in Example 1 and Comparative Example 1 After applying a cream of ~ 2, a replica of silicone material was prepared and the state of the wrinkles at the designated area was measured with a visiometer (SV60, Courage + Khazaka electronic GmbH, Germany) as an image analyzer , And the results are shown in Table 9 below. This result represents the average of the values obtained by subtracting the parameter values from the respective parameter values after 8 weeks by 8 weeks. That is, the negative value indicates that the wrinkle improving effect is higher.

R1 R2 R3 R4 R5 Example 1 -0.24 -0.20 -0.13 -0.08 -0.08 Comparative Example 1 0.27 0.25 0.21 0.04 0.03 Comparative Example 2 -0.25 -0.20 -0.12 -0.09 -0.08

R1: Difference between the maximum value and the minimum value of the wrinkle contour line

R2: The wrinkle contour line is arbitrarily divided into 5 squares, and the average of R1 values

R3: Maximum value of R1 divided by 5

R4: Average value obtained by subtracting the value of each valley and valley from the baseline of the wrinkle contour line

R5: Mean value of the value obtained by subtracting the outline of each wrinkle from the base line of the wrinkle contour line

As can be seen from the above Table 9, Example 1 containing the Dragon Blood resin extract as compared to Comparative Example 1 showed a large negative value in all items. In addition, compared with Comparative Example 2 containing adenosine, which is a wrinkle-improving functional ingredient, it showed negative values of the same level. Thus, it can be seen that the effect of improving the skin wrinkles of the cosmetic composition of the present invention is excellent.

[Test Example 7] Recovery effect of skin barrier function

In order to measure the skin barrier function recovery effect of Example 1, acetone was repeatedly applied to the skin of a hairless mouse to impair barrier function, and then the skin moisture loss (TEWL, transdermal water loss) (Sweden) evaporation meter (EP1), and when it reached 4.0 g / m 2 / h, the above Example 1 and Comparative Example 1 were applied to an area of 5 cm 2, and the skin moisture loss after 1, 2, 4 and 8 hours And the degree to which the barrier function was restored was evaluated by evaluating the degree of decrease. At this time, a normal lipid mixture (ceramide: 2: 1: 1 mixture of cholesterol: fatty acid) was used as a positive control. The skin moisture loss in the initial damaged barrier was 100%, and the changes over time were compared. The results are shown in Table 10 below.

sample Skin moisture loss After 1 hour After 2 hours After 4 hours After 8 hours Example 1 57 48 37 25 Comparative Example 1 87 79 76 73 Lipid mixture 65 45 38 24

As can be seen from the results of Table 10, Example 1 containing Dragon Blood resin extract had less skin moisture loss than Comparative Example 1, which did not contain the active ingredient, and had better skin barrier function recovery than the positive control lipid mixture Effect.

[Test Example 8] Test for moisturizing effect in human body

Forty adults, male and female, who were considered to be dry or dry skin, were divided into two groups, and each group was provided with Example 1 and Comparative Example 1 and applied on the face for 2 weeks each day for 4 weeks. TEWL was measured at constant temperature and humidity (24 ° C, relative humidity 40%) 2 weeks and 4 weeks after application and before application, and the moisture resistance was compared. The results are shown in FIG. In addition, Example 1 was applied to examine the moisture holding ability at the time of one application, and the skin moisture was measured with a corneometer for 6 hours. The results are shown in FIG.

<Trans Epidermal Water Loss (TEWL) Measurement Method>

Principle: The amount of water evaporating from the skin is calculated according to the area and time, and the amount of water evaporating is read by the temperature and humidity electronic sensor to measure the moisture of the skin.

1. Environmental condition: Keep the temperature and humidity constant, leave only a small number of persons besides the measurer and the subject, and block the air flow as much as possible.

2. Place the TEWL probe on the subject's measurement site.

3. Do not apply excessive force to the measurement site, lightly place the probe so that no marks are left on the skin, and make an angle of 90 degrees with the ground.

4. Measure the value of the skin's TEWL constant over time (takes 1 to 3 minutes).

5. Be careful not to let the temperature of the measurer pass by holding the handle of the probe as far away as possible.

6. Calculate the amount of water (g / m ² / h) that evaporates per hour per area.

As can be seen from the results of FIG. 1, when the skin of Example 1 containing the dragon-blooded resin extract was applied to the skin, the TEWL change value (moisture evaporation amount) with time was decreased. Thus, it was found that the moisturizing effect of the cosmetic composition of the present invention was excellent.

<Connio meter measurement method>

Principle: The amount of water present in the epidermis of the skin is measured by measuring the degree of ion of water using a sensor, numerically calculating the amount of moisture, and measuring the moisture content.

1. Place a probe of the corneometer on the skin area to be measured.

2. When the probe is pressed onto the skin, the electrical conductivity of the skin is quantified through the sensor and displayed on the screen.

3. Repeat the measurement with different measurement sites.

4. After one measurement, wipe the sensor with a kim wipe and measure again.

As can be seen from the results in FIG. 2, the increase in the amount of water in the skin was greater than that in the non-treated group in Example 1 containing the dragon-blood resin extract and the moisturizing effect of Example 1 was excellent Can be confirmed.

[Test Example 9] Usability evaluation

Twenty-four adult males and females who were thought to be dry or dry skin were divided into two groups and each group was provided with Example 1 and Comparative Example 1 and applied to the face for 2 weeks each day for 4 weeks.

After 4 weeks of use, the skin dryness improvement effect was evaluated through a questionnaire about usability. The results are shown in Table 11 below.

Application group Number of Respondents Very good Good usually Inadequate Example 1 6 5 One 0 Comparative Example 1 0 One 7 4

As can be seen from the results of Table 11, the person who applied the Example 1 felt that the skin dryness improvement effect and the moisturizing effect were more excellent than the person who applied the Comparative Example 1.

[Test Example 10] Whitening effect on human skin

The following experiment was carried out to examine the whitening effect of the cosmetics prepared in Example 1 and Comparative Example 1.

First, in 14 healthy men, an opaque tape with a hole of 1.5 cm in diameter was attached to the upper arm of the subject, and the UV rays (UVB) of about 1.5 to 2 times of the minimum erythema dose of each subject ) Was induced to induce blackening of the skin. The whitening cosmetic preparation prepared in Example 1 and Comparative Example 1 was applied twice a day (morning and evening) for two months each day, and after two months, a chromameter (Chromameter, Minolta CR-300).

The determination of the effect was made by determining the value "L" For reference, Korean skin color, which is not artificially burned, generally has a value of 50 ~ 70. Using a color difference meter (Minolta CR2002), the degree of blackness of the skin was measured to determine the effect. L * a * b * colorimetric system is used for color display, but L * value (brightness) is mainly used in the present invention as an index. The L * value was calibrated with a standard white plate, and the measurement was repeated five times or more at one site to measure the pigment deposit evenly. The difference in skin color (? L *) between the application start time (before application) and the completion time (two months after application) was calculated according to the following formula 2, and the results are shown in Table 12 below. The whitening effect is determined by comparing ΔL * between the sample application site and the control site. The higher the ΔL * value, the better the whitening effect.

Evaluation items Example 1 Comparative Example 1 The whitening effect (? L) 2.93 0.81

In the results of Table 12 above, the? L value of Example 1 containing the Dragon Blood resin extract was significantly higher than that of Comparative Example 1 not containing the Dragon Blood resin extract, It can be seen that the composition containing the dragon-blood resin extract provides excellent whitening effect.

[Test Example 11] Test for confirming skin turnover promotion effect in human skin

To investigate the skin turnover promoting effect of Example 1 and Comparative Example 1, the control group (untreated group) and the positive control group (treated with 1% lactic acid) according to the present invention, 28 healthy men and women 34 years old), the inside color of the upper part of the arm of the subject before the application of the test material was measured with a chromaticity meter, and artificial tanning was performed using a cream containing dihydroxyacetone (DHA). The color difference Were compared. Subsequently, a sample in which 0.2% by weight of 1% lactic acid used in the composition of Example 1 and Comparative Example 1 and used in the positive control was dissolved in a mixed solvent (water: ethanol: butylene glycol = 80: 15: 5) (Color change due to protein discoloration = skin turnover time) was measured by measuring the degree of discoloration on a daily basis with a chromaticity change meter and measuring the time taken to return to the original skin color. The skin turnover promotion rate was calculated by the following equation (3), and the results are shown in Table 13 below.

Here, the turnover time is the time taken for replacing the existing stratum corneum with a new stratum corneum, i.e., the time taken for the skin to return to its original shape after being tanned by DHA.

Test substance Turnover time (day) Turnover promotion rate (%) Control group 18.5 - Positive control group 12.5 32.4 Example 1 12.0 35.1 Comparative Example 1 15.5 16.2

The results of Table 13 show that the whitening cosmetic preparation of Example 1 according to the present invention promotes turnover over Comparative Example 1 and the control group and shows a shorter turnover period than the positive control group, Lt; RTI ID = 0.0 &gt; melanin &lt; / RTI &gt;

Hereinafter, a formulation example of the composition will be described, but it is to be understood that the present invention is not intended to be limited thereto but is specifically described.

[Formulation Example 1] Suspension emulsion lotion

Suspended emulsion lotion can be prepared with the composition shown in Table 14 below.

Constituent Content (% by weight) Methylparaben
Spices
Cetyl octanoate
Polyoxyethylene hardened castor oil
Polysiloxane
Tocopheryl acetate
ethanol
glycerin
Propylene glycol
Carboxyvinyl polymer
Dragon Blood Resin Extract
Purified water Suitable amount
Suitable amount
1.0
0.6
0.4
0.1
12.0
6.0
2.0
0.12
0.1
TO 100

[Formulation Example 2] Essence

Essences can be prepared with the composition shown in Table 15 below.

Constituent Content (% by weight) Methylparaben
Spices
Cetyl octanoate
Octyldodec-16
Coles-24 / Ceteth-24
Polysiloxane
Tocopheryl acetate
ethanol
glycerin
Xanthan gum
Propylene glycol
Carboxyvinyl polymer
Dragon Blood Resin Extract
Purified water Suitable amount
Suitable amount
1.0
0.5
0.2
0.4
0.1
6.0
15.0
0.07
4.0
0.12
0.1
TO 100

[Formulation Example 3] Nourishing cream

Nutritive creams were prepared with the compositions shown in Table 16 below.

Constituent Content (% by weight) Liquid paraffin
Cetostearyl alcohol
Wax
Polysiloxane
Chin type monostearate stearate
Stearic acid
Cetearyl glucoside
Polyoxyethylene sorbitan monostearate (20 EO)
Hydrogenated lecithin
Cetyl octanoate
Methylparaben
Propylparaben
Spices
Ethylenediamine sodium tetraacetate
glycerin
Carboxyvinyl polymer
Propylene glycol
Dragon Blood Resin Extract
Purified water 15.0
2.0
3.0
0.5
2.0
2.0
1.5
1.3
2.0
1.0
0.2
0.1
Suitable amount
0.02
5.0
0.10
2.0
0.5
TO 100

[Formulation Example 4]

A gel was prepared with the composition shown in Table 17 below.

Constituent Content (% by weight) antiseptic
Spices
Cetearyl glucoside
Cetyl octanoate
glycerin
Jojoba wax
Di-l-phenanthinol
ethanol
Carboxyvinyl polymer
Propylene glycol
Dragon Blood Resin Extract
Purified water Suitable amount
Suitable amount
1.5
1.0
5.0
3.0
1.0
7.0
0.6
3.0
0.5
TO 100

[Formulation Example 5] Pack

A pack was prepared with the composition shown in Table 18 below.

Constituent Content (% by weight) Methylparaben
Spices
Cetyl octanoate
Polyoxyethylene hardened castor oil
Polysiloxane
Cellulose sword
ethanol
glycerin
Propylene glycol
Polyvinyl alcohol
Dragon Blood Resin Extract
Purified water Suitable amount
Suitable amount
1.0
0.6
0.2
0.2
6.0
5.0
3.0
15.0
1.0
TO 100

Claims (7)

A cosmetic composition for skin whitening comprising an extract of Dragon Blood resin as an active ingredient. The cosmetic composition for skin whitening according to claim 1, wherein the dragon-blood resin extract is contained in an amount of 0.0001 to 20% by weight based on the total weight of the cosmetic composition. A cosmetic composition for enhancing skin moisturizing power comprising Dragon Blood resin extract as an active ingredient. The cosmetic composition according to claim 3, wherein the dragon-blood resin extract is contained in an amount of 0.0001 to 20% by weight based on the total weight of the cosmetic composition. delete delete delete

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