KR20100023372A - Cosmetic composition containing phyllanthus urinaria extrancts - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Phyllanthus urinaria extract as an active ingredient is provided to promote collagen synthesis and to suppress MMP-1(Matrix metalloproteinase-1) and cyclooxygenase-2 synthesis. CONSTITUTION: A cosmetic composition for anti-aging, anti-oxidation, and anti-wrinkle contains 0.001-10 weight% of Phyllanthus urinaria extract as an active ingredient. The extract is obtained using ethanol, methanol, butanol, ether, etheracetate, or chloroform. The cosmetic composition is used in the form of skin softener, astringent, nutrition lotion, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil or body essence.

Description

Cosmetic composition containing fox bead extract {Cosmetic composition containing Phyllanthus urinaria extrancts}

The present invention relates to a cosmetic composition having an anti-aging, antioxidant or anti-wrinkle effect, and more particularly, by containing a fox bead extract as an active ingredient, promoting collagen production in the skin, inhibiting MMP-1, and preventing DNA damage. It relates to a cosmetic composition that protects, inhibits the biosynthesis of cyclooxygenase-2, inhibits the biosynthesis of tumor necrosis factor-α, and has an anti-aging, antioxidant or antiwrinkle effect.

Human skin is changed by a number of internal and external factors as it ages. That is, internally, the secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells decreases, thereby reducing the biosynthesis of immune proteins and constituent proteins necessary for living organisms. Due to the increase in the amount of ultraviolet rays reaching the surface of the sun's rays and further increase environmental pollution, free radicals and free radicals, such as free radicals, skin thickness, wrinkles, elasticity is reduced only In addition, the color of the skin becomes dull, skin problems frequently occur, and blemishes, freckles and black mushrooms also increase, causing various changes.

As aging progresses, symptoms such as changes and decreases in the content and arrangement of collagen, elastin, hyaluronic acid and glycoproteins, which are constituents of the skin, appear and are subject to oxidative stress caused by free radicals and active harmful oxygen. In addition, cyclooxygenase-2 (Cox-2, cyclooxygenase), an enzyme that produces proinflammatory cytokine, which is known to cause inflammation, in most cells constituting the skin due to aging or ultraviolet rays. Biosynthesis of the enzyme increases the biosynthesis of matrix metalloproteinase (MMP), an enzyme that breaks down skin tissues by these inflammatory factors, and the production of nitric oxide (NO) by iNOS (inducible nitric oxide synthase). It is known to increase. In other words, the biosynthesis of the substrate material is reduced by the reduction of cellular activity and micro-inflammation due to naturally occurring endogenous aging, and by external factors such as the increase of stress caused by various harmful environments and the increase of free radical species caused by sunlight. As a result, decomposition and degeneration are accelerated, and the skin matrix is destroyed and thinned, resulting in various symptoms of skin aging. Therefore, a lot of research is being conducted on the active ingredient that can prevent and improve the phenomenon of aging.

Fox bead ( Phyllanthus) urinaria ), also called young or pearlescent, grows mainly in fields or grasses. It is 15 ~ 40cm high, reddish, and branches are spread. It has narrow wings on branches, leaves are alternately arranged in two rows, and look like feathery leafs. There are several triangular leaves with the eggs upside down. The leaves are oval, rounded at the ends, and flat at the edges. The leaf back is white and the petiole is short. Flowers are monogamous, bloom in July-August, and reddish brown. Six cases, three surgery, one pistil. Fruits are capsules, obovate, without sack, reddish brown, with lateral wrinkles. Similar to fox pockets but without fruit sacks, widely distributed in Korea, Japan, and tropical and subtropical regions. Fox beads also have therapeutic effects against species caused by enteritis, dysentery, infectious hepatitis and nephritis, urinary tract infections, nominal, markers of infants, eyeballs, soles and cotton swelling, and can be added to cosmetics for whitening effects. It is known.

Korean Patent Laid-Open Publication No. 2004-59004 discloses a skin whitening composition containing a plant extract having melanin production inhibitory activity containing at least one selected from the group consisting of pearl leaf extracts, cucurbita extracts, and mixtures thereof as an active ingredient. Is disclosed.

In addition, Japanese Patent Application Laid-Open No. 8-12566 discloses pearl leaf extract ( Phyllanthus) niruri L.), do Atlantico (Piper elongatum Vahl. & Piper ungustifolium ) and Western Nettle ( Urtica) A tyrosinase activity inhibitor containing a combination of one or two or more selected from the group consisting of urens ) is disclosed .

Thus, the present inventors while studying the anti-aging cosmetics to inhibit skin aging, fox bead extract promotes collagen production in the skin, inhibits MMP-1, protects DNA damage, biosynthesis of cyclooxygenase-2 The present invention was found to exhibit anti-aging, antioxidant or anti-wrinkle effect by inhibiting the biosynthesis of tumor necrosis factor-α.

Accordingly, it is an object of the present invention to promote collagen production in the skin, to inhibit MMP-1, to protect DNA damage, to inhibit the biosynthesis of cyclooxygenase-2, to inhibit the biosynthesis of tumor necrosis factor-α It is to provide a cosmetic composition having an anti-aging, antioxidant or anti-wrinkle effect.

In order to achieve the above object, the present invention provides a cosmetic composition for anti-aging containing the fox bead extract as an active ingredient.

In addition, the present invention provides an antioxidant cosmetic composition containing the fox bead extract as an active ingredient.

In addition, the present invention provides a cosmetic composition for improving wrinkles containing fox bead extract as an active ingredient.

The cosmetic composition according to the present invention contains the fox bead extract as an active ingredient to promote collagen production in the skin, inhibit MMP-1, protect DNA damage, inhibit the biosynthesis of cyclooxygenase-2, tumor By suppressing the biosynthesis of necrosis factor-α it was possible to provide a cosmetic composition having an anti-aging, antioxidant or anti-wrinkle effect.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition for anti-aging, antioxidant or wrinkle improvement containing the fox bead extract as an active ingredient.

The fox bead extract used in the present invention may be prepared by extracting the fox bead with water or an organic solvent by a method well known in the art. The organic solvent used in the present invention may be one or more selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, or a mixed solvent of these organic solvents and water may be used. Preferably 80% ethanol can be used.

The cosmetic composition according to the present invention may contain the fox bead extract in an amount of 0.001 to 10% by weight based on the total weight of the composition, preferably 0.01 to 5% by weight. If the content of the fox bead extract is less than 0.001% by weight, anti-aging, anti-oxidation and anti-wrinkle effect may not be obtained by the extract.

Cosmetic compositions according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) obtained by dispersing an oil phase in solutions, gels, solids, pasty anhydrous products, aqueous phases and It may be provided in the form of a nonionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to the present invention is a fatty substance, an organic solvent, a dissolving agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic or nonionic. Such as type emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics. It may contain adjuvants conventionally used in the cosmetic or dermatology field. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological arts.

The cosmetic composition according to the present invention is not particularly limited in the formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, It may be formulated into cosmetics such as cleansing water, packs, powders, body lotions, body creams, body oils and body essences.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to Examples and Test Examples. However, these experimental examples and examples are provided only for the purpose of illustration in order to help the understanding of the present invention is not limited to the scope and scope of the present invention.

Example 1 Preparation of Fox Bead Extract

2 l of a 70% ethanol aqueous solution of 4 times the weight ratio was added to 0.5 kg of dry fox beads, and the mixture was extracted three times under reflux, and then deposited at 25 ° C. for 3 days. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain a fox bead extract.

Test Example 1 Antioxidant Effect of Fox Bead Extract

Antioxidant effect was investigated through an experiment comparing the ability to remove reactive oxygen species (ROS) generated in the cells by ultraviolet (UV) irradiation, the results are shown in FIG. As a positive control, trolox, which is generally used to compare antioxidant effects, was used, and a group without treatment of test substance was used as a control.

In the results of Figure 1, the fox bead extract of Example 1 according to the present invention significantly erases the active oxygen generated by UV compared to the control in the human HaCaT keratinocytes monolayer culture system (human HaCaT keratinocytes monolayer culture system) It showed an effect. This was an excellent activity similar to trolox used as an activity indicator of the antioxidant substance, through which the fox bead extract according to Example 1 of the present invention significantly eliminates free radicals that cause skin aging by wrinkles of the skin It was confirmed that it can have an effect of preventing the production and elasticity degradation.

Test Example 2 Inhibitory Activity against DNA Oxidative Damage by Single Cell Gel Electrophoresis (SCGE, Comet Assay)

After culturing human fibroblasts for 24hr, the fox bead extract and vitamin E of Example 1 were treated for each concentration, and after 12hr, H ₂ O ₂ (final concentration 10-3M), which was a positive control, was treated by electrophoresis. The stained result was observed by fluorescence microscope. At this time, the length (um) of the tail resulting from DNA cleavage in each of 25 cells was analyzed using KOMET 3.1 (Kinetic Imaging, England), an image analyzer, and the results are shown in Table 1 below. Indicated.

Fox Bead Extract Inhibits Vitamin E Oxidative Damage Test substance Tail length (μm) % Inhibition H ₂ O ₂ 110.6 - H ₂ O ₂ + Example 1 (10 ppm) 65.8 24.4 H ₂ O ₂ + Example 1 (50 ppm) 55.7 33.2 H ₂ O ₂ + Example 1 (100 ppm) 42.1 44.9 H ₂ O ₂ + Vitamin E 10 ppm 60.6 38.9

In the results of Table 1, the fox bead extract of Example 1 by cell gel electrophoresis showed significant oxidative DNA damage inhibitory activity, whereby the fox bead extract according to the present invention is a hydroxyl radical (.OH) It was confirmed that it acts as a DNA oxidative damage inhibitor that inhibits the DNA single strand break.

Test Example 3 Type I Procollagen Assay

Human fibroblasts were cultured in a 12-hole plate incubator at a concentration of 10 ⁵ and then replaced with a medium containing 1 ppm and 10 ppm of fox bead extract of Example 1. On day 3 of culture, the cells were harvested and the amount of type I procollagen produced by ELISA was quantified, and the results are shown in FIG. 2. The results were calculated as a comparison with the measured value of the control group that does not contain the fox bead extract of Example 1 as 100, TGF-b was used as a positive control.

As shown in the results of Figure 2, the fox bead extract of Example 1 in the normal human fibroblast monolayer culture system showed an effect of markedly promoting the production of type I procollagen compared to the control group . In other words, the fox bead extract of Example 1 was able to suppress the decrease in collagen production caused by aging of the human skin, and showed a wrinkle improvement effect.

Test Example 4 Analysis of MMP-1 Expression Inhibition

Human fibroblasts were cultured in a 12-hole plate incubator at a concentration of 10 ⁵ and then irradiated with ultraviolet B at 40 mJ / cm 2, and then replaced with a medium containing 1 ppm and 10 ppm of fox bead extract of Example 1. The cells were harvested on the second day of culture (harvest) to quantify the amount of MMP-1 (matrix metalloproteinase I) produced by the ELISA method, and the results are shown in FIG. 3. The result was calculated as a comparison with the value measured by the UV control group containing no test substance 100, the positive control group was TGF-b.

As shown in the results of Figure 3, the fox bead extract of Example 1 according to the present invention in the human normal fibroblast monolayer culture system significantly inhibited the expression of MMP-1 induced by UVB 40 mJ / ㎠ . Therefore, the fox bead extract of Example 1 according to the present invention inhibits the biosynthesis of the skin tissue degrading enzyme MMP-1 caused by internal aging or external environmental factors, inhibits skin aging and is effective in improving wrinkles It was.

Test Example 5 Effect of Inhibiting Biosynthesis of Cyclooxygenase-2 (COX-2) by Ultraviolet Rays

Human fibroblasts were cultured in a 12-hole plate incubator at a concentration of 10 ⁵ and then irradiated with UVA at 15 J / cm 2, and the fox bead extract of Example 1 was replaced with a medium containing 0.1pp, 1ppm and 10ppm, respectively. Cells were harvested on the second day of culture (harvest) and the amount of cyclooxygenase-2 (COX-2) produced was quantified using the Western blot method. The UV control group containing no test substance was calculated as 100 and compared with the value measured by a densitometer, and the results are shown in Table 2 below.

Test substance (concentration: ppm) COX-2 biosynthesis (%) Example 1 10 15 One 28 0.1 73 Control 100

From the results of Table 2, the fox bead extract of Example 1 concentration-dependently reduced the biosynthesis of cyclooxygenase-2 by ultraviolet light, skin tissue degradation by the prostaglandin E2 (PGE2) product of COX-2 Could be prevented.

Test Example 6 Inhibition of Biosynthesis of Tumor Necrosis Factor Alpha (TNF-α) by UV Light

Human keratinocytes were cultured in a 12-hole plate incubator at a concentration of 10 ⁵ and then irradiated with UVB at 30mJ / cm 2, and the fox bead extract of Example 1 was replaced with a medium containing 0.1pp, 1ppm and 10ppm, respectively. After 6-24 hours of culture, the cells were harvested (harvest) and the amount of tumor necrosis factor alpha (TNF-α) generated using the ELISA (Pharmingen 555212) method was quantified. The results calculated by comparison with the measured values are shown in Table 3 below.

Test substance (concentration: ppm) TNF-α biosynthesis (%) Example 1 10 16 One 38 0.1 72 Control 100

From the results of Table 3, the fox bead extract of Example 1 according to the present invention may be caused by the biosynthesis of tumor necrosis factor alpha by reducing the concentration of biosynthesis of tumor necrosis factor alpha (TNF-α) by ultraviolet radiation It was confirmed that skin aging can be prevented beforehand.

Example 2 and Comparative Example 1

Nutritional creams of Example 2 and Comparative Example 1 were prepared in a conventional manner according to the composition shown in Table 4 below (unit: wt%).

ingredient Example 2 Comparative Example 1 Fox Bead Extract of Example 1 0.1 - Beeswax 10 10 Polysorbate 60 1.5 1.5 Sebum 60 Cured Castor Oil 2 2 Sorbitan sesquioleate 0.5 0.5 Liquid paraffin 10 10 Squalane 5 5 Caprylic / Capric Triglycerides (Estasan: Uniqema) 5 5 glycerin 5 5 Butylene glycol 3 3 Propylene glycol 3 3 Triethanolamine 0.2 0.2 Purified water Remaining amount Remaining amount

[Test Example 7] skin wrinkle improvement effect on human skin

Forty subjects with facial wrinkles of 30-50 years old were subjected to Example 2 on the left side of the subject and Comparative Example 1 on the right side for 3 months, and then the skin wrinkle improvement effects were compared and evaluated. The skin condition of both sides of the face before the use of the cream was measured and the same site was remeasured three months after the use of the cream. In the constant temperature and humidity room of 24 ° C. and 40% RH, the wrinkles at the tail of the eyes were replicated, and skin wrinkles were measured using a Visiometer system (C + K). The amount of change in skin wrinkles was calculated according to Equation 1 below.

In Equation 1, "Tdi" is a measurement site value at D90, and "Tdo" is a measurement site value at D0.

As a result of the calculation according to Equation 1, the skin wrinkle of the site using Comparative Example 1 showed a decrease of 6.5 ± 4% (mean ± standard deviation), while the skin wrinkle of the site using Example 2 was 39 ± 11%. It showed a reduction of, showing an excellent skin wrinkle improvement effect.

Test Example 8 Skin Elasticity Improvement Effect in Human Body

Forty women over 30 years old were divided into two sets, and each set of nutritional creams of Example 2 and Comparative Example 1 was applied to the face twice a day for 12 weeks, followed by a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany) to measure the skin elasticity improvement effect, the results are shown in Table 5 below. The results in Table 5 refer to the properties of skin viscoelasticity of the skin elasticity tester.

Test substance Skin elasticity effect Example 2 0.46 ± 0.12 Comparative Example 1 0.29 ± 0.09

As shown in the results of Table 5, when using the nutrition cream of Example 2 containing the fox bead extract of Example 1 showed about 150% skin elasticity enhancing effect.

As described above, it could be confirmed that by using Example 2 containing the fox bead extract according to the present invention, skin wrinkles and skin elasticity could be improved to contribute to inhibiting and delaying skin aging.

Formulation Example 1 Flexible Cosmetic (Skin Lotion)

Ingredient Content (% by weight) Purified water Remaining amount Fox Bead Extract of Example 1 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1

Formulation Example 2 Nutritious Longevity (Milk Lotion)

Ingredient Content (% by weight) Purified water Remaining amount Fox Bead Extract of Example 1 0.1 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Montana 202 (Manufacturer: Seppic) 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2

Formulation Example 3 Massage Cream

Ingredient Content (% by weight) Purified water Remaining amount Fox Bead Extract of Example 1 0.1 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Montana 202 (Manufacturer: Seppic) 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2

[Formulation Example 4] Pack

Ingredient Content (% by weight) Purified water Remaining amount Fox Bead Extract of Example 1 0.1 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Fiji-12 nonylphenyl ether 0.3 Polysorbate 60 0.3

1 is a graph showing the active oxygen removal activity of the fox bead extract of Example 1.

Figure 2 is a graph measuring the collagen production amount of the fox bead extract of Example 1.

Figure 3 is a graph measuring the inhibitory effect of MMP-1 biosynthesis of the fox bead extract of Example 1.

Claims (5)

Anti-aging cosmetic composition containing the fox bead extract as an active ingredient. Antioxidant cosmetic composition containing the fox bead extract as an active ingredient. Cosmetic composition for improving skin wrinkles containing fox bead extract as an active ingredient. The cosmetic composition according to any one of claims 1 to 3, wherein the cosmetic composition contains 0.001 to 10% by weight of the fox bead extract based on the total weight of the composition. According to any one of claims 1 to 3, wherein the cosmetic composition is a softening longevity, astringent longevity, nourishing longevity, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, Cosmetic composition, characterized in that it has a formulation selected from the group consisting of a pack, powder, body lotion, body cream, body oil and body essence.

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