KR20130001441A - Cosmetic composition containing scoria - Google Patents

Abstract

PURPOSE: A cosmetic composition containing scoria is provided to suppress 5 alpha-reductase activation and to promote hair growth. CONSTITUTION: A cosmetic composition for promoting hair growth contains scoria as an active ingredient. A cosmetic composition for anti-aging contains scoria as an active ingredient. The composition further contains a Cryptomeria japonica extract, a Brassica camperstris(rapeseed) flower extract, a Bambusa vulgaris extract, or a Camellia sinensis leaf extract. The composition is used for suppressing 5alpha-reductase activation and promoting collagen biosynthesis.

Description

Cosmetic composition containing volcanic stone clusters {Cosmetic composition containing scoria}

The present invention relates to a cosmetic composition containing a volcanic stone cluster, and more particularly, to a cosmetic composition containing volcanic stone cluster as an active ingredient to promote hair growth and to provide an anti-aging effect through the synthesis of collagen. .

5α-Reductase is present in testosterone-reactive tissues such as sebaceous glands, hair follicles, prostate, and epididymis. As an enzyme involved, its conversion requires NDPH (NADPH). Testosterone is involved in skeletal muscle growth and spermatogenesis, and dihydrotestosterone is involved in tissues in acne, sebum and prostatic hyperplasia (Diane et al; J. Invest Dermatol. 775-778, 1995. Bruchovsky, N et al; JBC 243, 2112-2121, 1968). In addition, in the sebaceous glands of the skin, testosterone is converted to dihydrotestosterone by 5α-reductase, binds to a receptor protein in the cytoplasm, enters the nucleus, activates sebaceous gland cells, and promotes differentiation by stimulating differentiation in sebaceous glands. (Diane et al; J. Invest Dermatol. 105; 209-214) and excess secreted sebum causes acne and male hair loss. In particular, many hair follicle cells provide a cause for hair loss because dihydrotestosterone binds to hormone receptors better than testosterone.

Active oxygen species act as a causative agent of skin aging in particular. The skin continues to undergo photochemical reactions that produce free radicals when exposed to ultraviolet light. These reactive oxygen species lead to skin cell and tissue damage. They break the antioxidant defenses made up of antioxidant and non-enzymatic antioxidants, shifting the oxidant / antioxidant balance towards oxidation. As a result, continued oxidative stress leads to lipid peroxidation, protein oxidation, activation of proteolytic enzymes that destroy skin components, chain cleavage and abnormal cross-linking of collagen elastin, an elastic fiber, cleavage of hyaluronic acid chains, promotion of melanogenesis, Causing damage to biological components such as DNA oxidation. Damage to these biological components leads to various aging phenomena such as wrinkle formation, reduced elasticity, and widening pores.

Accordingly, the present inventors have found that volcanic stone clusters can inhibit the activity of 5α-reductase and increase the production of collagen, thus completing the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition having a hair growth effect while preventing hair loss.

In addition, another object of the present invention is to provide a cosmetic composition having an anti-aging effect by promoting the production of collagen.

In order to achieve the above object, the present invention provides a cosmetic composition for promoting hair growth containing volcanic stone cluster (Scoria) as an active ingredient.

The present invention also provides an anti-aging cosmetic composition containing a volcanic stone cluster as an active ingredient.

The cosmetic composition provided by the present invention contains volcanic stone clusters, and when applied to the skin, inhibits the activity of 5α-reductase, thereby reducing hair loss and promoting hair growth, and also preventing wrinkles through collagen synthesis. It promotes skin elasticity and provides anti-aging effects.

As used herein, the term "promoting hair growth" means not only promoting the production of new hair, but also ensuring that existing hair grows healthy, and prevents the phenomenon of hair falling off from the scalp and the condition of the hair becoming thin or thin. And restraint.

The present invention contains a volcanic stone cluster (Scoria) as an active ingredient to provide a cosmetic composition for promoting hair growth and anti-aging.

The volcanic stone (Scoria) used as an active ingredient in the present invention is a kind of volcanic ash present in Jeju Island in Korea. It is known that it is produced when a parasitic volcanic eruption occurs throughout Jeju Island. The reserves in Jeju Island are about 20 billion m ³ Is estimated to be almost infinite resources. Volcanic stone cluster is a natural material that can combat E. coli of 92 ~ 93% of far infrared ray, 97% of deodorization rate, and 99.9% of antibacterial fungus in its natural state, and it is not comparable to jade, ocher, elvan, germanium which are widely used in recent years. It is a natural material with the best features.

The volcanic stone cluster of the present invention can be obtained by a method conventional in the art, and the production method thereof is not particularly limited. For example, clusters collected in areas not contaminated with pesticides or the like may be obtained by pulverizing various particle sizes according to the use to remove impurities and dry heat sterilizing at a temperature of 150 ° C. or higher.

The cosmetic composition according to the present invention further comprises at least one selected from the group consisting of cedar (Cryptomeria japonica) extract, rape (Brassica camperstris (rapeseed) flower) extract, bamboo (Bambusa vulgaris) extract and green tea (Camellia sinensis leaf) extract It may contain.

Cedar (Cryptomeria japonica) used in the present invention grows well in a valley with an average annual temperature of 12 to 14 ° C. and rainfall of 3,000 mm and reaches 40 m in height and 1 to 2 m in diameter. The bark is reddish brown, split vertically, with dense branches and leaves, and becomes a cone-shaped tree. Leaves are curved needle-shaped, arranged in a spiral shape, and do not fall off when they dry. Hermaphrodite flowers bloom in March. The male flower is gathered at the end of a small branch, and the female flower is a ball-shaped one at the end of a short branch. Fruits are coniferous, woody, almost round, 2-3cm long. Fruit slices are thick, with sharp processes at the end. Seeds are 2-6 pieces at the bottom of the fruit pieces, long oval, with narrow wings around. Cedar wood has various variations depending on the color and splitting of the bark, tree type, the angle of the branches, the color and form and length of the leaves, the degree of baking, and the place of growth. There are horticultural varieties and afforestation varieties, and because of their long age, there are many varieties with various legends.

The rape flower (Brassica camperstris (rapeseed) flower) used in the present invention is a mustard and biennial herbaceous flowers bloom in March-April in turn and hang on the ends of branches. The height is 80 ~ 130cm and the surface is smooth and green. In the main stem, about 15 primary side branches come out, and 2 to 4 secondary side branches emerge from this branch. The leaves are lanceolate and have a dull tip. The leaves on the lower stem have long petioles and the leaf edges are deeply divided. The leaves on the upper stem do not have petioles, they surround the stem and their ends are thin. The leaves of Western species are thick and leathery (leather-like texture). The common species is light green and the leaves are non-leather. Stems usually have 30 to 50 leaves. Seeds contain 38-45% oil, which is edible oil containing 15-20% soluble nitrogen and 20% protein, which is consumed next to soybean oil and is used as salty feed or fertilizer. Flowers are wheat plants, and fatty oils separated from seeds are used as meat products, oil-based solvents, or machine lubricants.

Bamboo (Bambusa vulgaris) used in the present invention is a generic term for perennial plants belonging to the monocotyledonous rice plant (Paceae), the family Bambusoideae, sometimes referred to as only the tall king genus bamboo. It is distributed throughout the world but grows mainly in the tropics, especially in the Asian monsoon region. It is widely distributed in south central and Jeju islands in Korea. In the case of tall royal genus, it can grow up to 30m in height and 30cm in diameter. Stems are round, round, and hollow. The growth method is that the stem in the ground extends to the side and grows naturally by removing roots and sprouts from the node. The leaves are narrow and long. Likes wet land and grows fast. It also rarely blooms, but when it blooms, I bloom all at once in a bamboo field. The flower of bamboo is a kind of mutation that is not related to the propagation of bamboo. It is also known as a flowering disease or a natural highland. The flowering period varies from 3 years, 4 years, 30 years, 60 years, 120 years, and so on. Bamboo has a wide variety of uses, ranging from building materials, furniture, fishing rods, plant supports, to basketwork such as baskets, and is also planted for ornamental purposes. Young sprouts are cooked and eaten with herbs.

In addition, the green tea (Camellia sinensis leaf) used in the present invention inactivates the enzyme by treating the oxidase present in the tea leaves with heat or steam using the shoots or leaves of Camellia sininsis of Camellia family. It is made by. Green tea has not only been consumed as a symbol tea since BC, but as the pharmacological mechanism of various ingredients contained in green tea has recently been revealed, its value has been recognized. It contains a large amount of active ingredients such as polyphenols, caffeine, amino acids, and vitamin C. It has been found to be effective in hangover and nicotine detoxification, fatigue recovery, cardiovascular action, and skin care. Antioxidant, anticancer, blood cholesterol lowering, anti-aging, heavy metal detoxification, caries prevention and bad breath removal effect has been attracting much attention.

Cedar extract, rapeseed extract, bamboo extract and green tea extract of the present invention can be prepared by extraction by a method known in the art, the method is not particularly limited. Preferably, the extraction may be performed using an extraction solvent in which water and 1,3-butylene glycol are mixed in a weight ratio of 1: 1. At this time, the extraction temperature is preferably 40 ℃, it can be extracted for 6 to 24 hours. If the extraction temperature and the extraction time is out of the extraction efficiency may be lowered or a change of components may occur.

The cosmetic composition according to the present invention contains volcanic stone clusters, cedar extract, rape blossom extract, bamboo extract and green tea extract in an amount of 0.0001 to 80.0% by weight, more preferably 0.01 to 50.0% by weight, based on the total weight of the composition. If the content is less than 0.0001% by weight, the efficacy and effect are insignificant to the above components, and if the content is more than 80.0% by weight, the increase in efficacy is not large compared to the increase in content, which is rather inefficient.

The cosmetic composition provided by the present invention inhibits the activity of 5α-reductase, which promotes the production and activity of dihydrotestosterone, which induces hair loss when applied to the skin, thereby promoting hair growth and preventing hair loss. . In addition, scalp stimulation may be alleviated, such as reduction of irritation such as itching and stinging of the scalp and scalp inflammation, and the scalp stimulation includes stimulation that may appear during chemical treatment such as staining and perming. In addition, the composition of the present invention can strengthen the hair root and prevent hair thinning due to scalp damage.

In addition, the cosmetic composition provided by the present invention can prevent skin aging by promoting collagen synthesis when applied to the skin to increase skin elasticity and inhibit the formation of wrinkles.

Cosmetic compositions according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) obtained by dispersing an oil phase in solutions, gels, solids, pasty anhydrous products, aqueous phases and It may be provided in the form of a nonionic vesicle dispersant or in the form of a cream, skin, lotion, powder, ointment, spray, pack, or cone stick. It may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

In addition, the composition for promoting hair growth of the present invention is not particularly limited in formulating, cosmetic composition having a formulation such as hair tonic, hair nourishing longevity, scalp treatment, hair treatment, hair rinse, hair shampoo, hair lotion, etc. Can be.

In addition, in each formulation, other ingredients in addition to the above-mentioned essential ingredients may be appropriately selected and blended by those skilled in the art without difficulty according to the kind or purpose of use of other external preparations. For example, the cosmetic composition according to the present invention is a fatty material, an organic solvent, a dissolving agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ion Any commonly used in type or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatology field as other ingredients. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological arts.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to test examples and examples. However, these test examples and examples are provided only for the purpose of illustration in order to help the understanding of the present invention is not limited to the scope and scope of the present invention.

[Test Example 1] 5α-reductase activity inhibitory effect

Mature Sprogue-Dawley male rats (7-8 weeks old) were killed with diethyl ether, and the abdominal prostate was removed to remove connective tissue and buffered (0.32 M sucrose, 0.1 mM dithiothreitol, 20 mM). sodium acetate) was added to the finely chopped and suspended in a grinder. The suspension was centrifuged to obtain supernatant, partially purified 5α-reductase, and the activity inhibition experiment of this enzyme was performed.

Taking a portion of the supernatant obtained above was placed in a buffer containing 0.2M monobasic acid and 0.2M dibasic acid, is reacted with isotope substrate (testosterone) marked with ³ H measure the amount of dihydrotestosterone product It was. The reaction solution was prepared by adding 1 mM dithiothreitol, 40 mM sodium phosphate (pH 6.5), 50 μM NADPH, [1,2,6,7-3H] testosterone / testosterone (2.2X10 ⁹ mol), and enzyme suspension (0.8 mg). The volume was brought to 565 μl.

The sample was dissolved in 10% ethanol so as to be 0.1%, and 5.65 L of the above reaction solution was added to make the final concentration to 0.001%. The same volume of solvent was used as a control, and riboflavin was used as a positive control.

Volcanic stone clusters (trade name: Volcanic stone clusters, manufacturer: Bioland Co., Ltd.) used raw materials processed in powder form, and cedar extract, bamboo extract, green tea extract, and rape blossom extract (manufacturer: Bioland Co., Ltd.). It was obtained by putting 100 g of dry weight of each sample into each flask, cold extraction with 3 l of distilled water for 1 day, extraction, and filtration to remove debris.

The reaction was started with addition of the enzyme suspension and proceeded at 37 ° C. for 30 minutes, followed by extraction by adding 1 ml of ethyl acetate. 100 μl of ethyl acetate was extracted from silica plastic sheet kiesel gel 60 254 (Silica plastic sheet kieselgel). The developing solvent system on F254) was developed with acetate-cyclohexane (1: 1).

After drying the plastic sample in air, a bath system was used to measure the isotope amount. The dried plastic sheet and the x-ray film were put together in a bath cassette to allow isotopes of testosterone and dihydrotestosterone remaining on the film after one week. The quantification was measured. The results are shown in Table 1 below.

sample T (dpm) DHT (dpm) Conversion Rate (%) Inhibition rate (%) Volcanic Stone Cluster 8750 2873 22.4 53.3 Volcanic Stone Cluster + Cedar Extract (1: 1) 9150 2890 21.1 56.04 Volcanic Stone Cluster + Rape Blossom Extract (1: 1) 9320 2930 19.7 58.9 Volcanic Stone Cluster + Bamboo Extract (1: 1) 9280 2910 19.2 60.0 Volcanic Stone Cluster + Green Tea Extract (1: 1) 9420 2940 20.2 57.9 Volcanic Stone Cluster + Cedar Extract + Rape Flower Extract + Bamboo Extract + Green Tea Extract (1: 1: 1: 1: 1) 9830 2970 18.7 61.0 Control group 7430 5935 48.0 - Positive control group 7500 2530 29.7 38.1

(1) T: 3H Radio activity in the testosterone region

(2) DHT: 3H radioactivity in the dihydrotestosterone region

(3) Conversion rate: radioactivity / total radiation to the DHT region

(4) Inhibition rate: 100 * (control conversion-sample conversion) / control conversion

From the results of Table 1, the volcanic stone cluster of the present invention converts testosterone to dihydrotestosterone, binds to a receptor protein in the cytoplasm, enters the nucleus, activates sebaceous gland cells and promotes differentiation, thereby over secreting sebum in sebaceous glands. It was found that blocking the conversion of testosterone to dihydrotestosterone by effectively inhibiting the ductase activity.

In addition, it was confirmed that the use of volcanic stone clusters and cedar extracts, rape blossom extracts, bamboo extracts, green tea extracts, or mixtures thereof showed a higher inhibitory effect of 5α-reductase activity than volcanic stone clusters alone.

Therefore, the mixture of volcanic stone cluster and volcanic stone cluster and cedar extract, rape blossom extract, bamboo extract or green tea extract of the present invention has an excellent effect of inhibiting the activity of 5α-reductase, which is effective in suppressing the excessive secretion of sebum.

[Referential Example 1]

According to the composition described in Table 2 below, Examples 1 to 6 and Comparative Example 1 of the lotion formulation were prepared (unit: wt%).

Raw material name Example
One Example
2 Example
3 Example
4 Example
5 Example
6 Comparative Example
One 1.Cetearyl Alcohol 1.0 1.0 1.0 1.0 1.0 1.0 1.0 2.lipophilic glyceryl stearate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 3. Glyceryl Stearate SE 1.5 1.5 1.5 1.5 1.5 1.5 1.5 4.phytoscualan 3 3 3 3 3 3 3 5.hydrogenated polydecene 2 2 2 2 2 2 2 6.Dimethicone 0.5 0.5 0.5 0.5 0.5 0.5 0.5 7.Polysorbate 60 One One One One One One One 8.Sorbitan sesquioleate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 9.Methylparaben 0.1 0.1 0.1 0.1 0.1 0.1 0.1 10.propylparaben 0.05 0.05 0.05 0.05 0.05 0.05 0.05 11. Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 12.butylene glycol 5 5 5 5 5 5 5 13.Polyacrylate-13 * Polyisobutene * Polysorbate 20 0.5 0.5 0.5 0.5 0.5 0.5 0.5 14. Volcanic Stone Cluster One - - - - - - 15. Molten Stone Cluster + Cedar Extract (1: 1) - One - - - - - 16. Volcanic Stone Cluster + Rape Blossom Extract (1: 1) - - One - - - - 17. Volcanic Stone Cluster + Bamboo Extract (1: 1) - - - One - - - 18. Volcanic Stone Cluster + Green Tea Extract (1: 1) - - - - One - - 19. Volcanic Stone Cluster + Cedar Extract + Rape Flower Extract + Bamboo Extract + Green Tea Extract (1: 1: 1: 1: 1) - - - - - One -

<Production method of Examples 1 to 6 and Comparative Example 1>

1) The 11-19 components were uniformly mixed while heating to 70 degreeC, and the water phase part was produced.

2) The above components 1 to 10 were uniformly mixed while heating to 70 ° C to prepare an oil phase part.

3) The oily part of 2) was added to the aqueous part of 1) and homomixed at 7,200 rpm for 6 minutes.

4) The mixture of 3) was cooled to room temperature.

Test Example 2 Stimulation Relief Efficacy Test

In order to evaluate the efficacy of stimulating relaxation treatment by stimulation of hair dye, MEST method using mouse was applied. The test method is as follows.

Seven 6-week-old BALB / c rats were purchased and divided into 7 groups, which were set up with a 10-day quarantine and adaptation period. Hair was removed from the abdomen of the rat, and 2.5% paraphenylenediamine (PPD) was applied for 3-5 days. After a week of rest, hair dye was applied to the rats' ears and wiped off after 30 minutes. After having a rest period of one week again, the compositions of Examples 1 to 6 and Comparative Example 1 were applied twice at an hourly interval. 24 hours after the application of the hair dye, the compositions of Examples 1 to 6 and Comparative Example 1 were applied twice. After 24 hours, the weight of the ear was measured, and the weight of the pathologic ear was measured by H & E staining. The hair dye formulation is shown in Table 3 below, and the measurement results are shown in Table 4 below.

ingredient Content (% by weight) Ingredient 1 Purified water To 100 Sodium sulfite 0.5 Asvo Kick Acid 0.5 Polyoxyethylene uryl ether 4.0 Polyoxyethylene oleyl ether 4.0 Cetostearyl alcohol 8.0 p-phenylenediamine 0.5 Resorcinol 0.5 Monoethanolamine Suitable amount ammonia Suitable amount 2nd ingredient Purified water 0.1 Hydrogen peroxide (30%) 17.0 Phosphoric Acid 0.1

Treated group Ear weight (mg) Ear thickness (㎛) Example 1 9.75 ± 0.943 266.12 ± 24.568 Example 2 9.46 ± 0.875 252.24 ± 22.392 Example 3 9.52 ± 0.468 254.46 ± 23.493 Example 4 9.43 ± 0.678 250.08 ± 21.498 Example 5 9.55 ± 0.554 255.48 ± 24.487 Example 6 9.24 ± 0.721 224.24 ± 21.792 Comparative Example 1 14.39 ± 0.242 322.75 ± 21.883

As can be seen in Table 4, when the pretreatment with the compositions of Examples 1 to 6 according to the present invention can be confirmed that the ear weight and the thickness of the ear is significantly reduced to reduce the inflammation. Thereby, it can be judged that the composition by this invention has the effect of alleviating the inflammation by a hair dye.

Test Example 3 In vivo Itching Inhibition Test

The anti-itch effect of the composition according to the present invention was tested. Hairless mice were used to facilitate skin application of the compositions of Examples 1 to 6 and Comparative Example 1. The hairless mice were all 7-week-old males, and all groups used 8 hairless mice, respectively, for comparison with the negative control group.

Treatment of the external preparation was carried out by 150 μl of the compositions of Examples 1 to 6 and Comparative Example 1 and spreading them widely on the back of the hairless mice. The treatment was performed twice a day for 1 day divided into the morning and afternoon, and last 30 days before the injection of itching agent in the morning on the second day.

The itch test allowed 30 minutes to acclimatize a hairless mouse into an observation clear cage 30 minutes before the itch-injecting material was injected. At the end of the acclimatization time, compound 48/80 (compound 48/80), an itch-causing substance, was dissolved in physiological saline and intradermal injection was applied to the back skin of the adapted hairless mice. . Immediately after the injection, the hairless mice were placed in the cage for observation, and video was taken for 30 minutes. The number of scratches on the site where the itch-inducing substance was injected into the hind legs was measured and displayed in numbers. Except for scratching by the forefoot or biting by mouth, only the action of scratching the injection site with the hind feet was considered as an indication of the itch behavior, and the measurement results are shown in Table 5 below.

Treated group Itching Agent Average number of scratches in 30 minutes Negative control group Saline solution 16.7 ± 27.9 Example 1 C48 / 80 122.4 ± 24.8 Example 2 C48 / 80 120.3 ± 21.5 Example 3 C48 / 80 119.7 ± 22.4 Example 4 C48 / 80 121.2 ± 22.5 Example 5 C48 / 80 118.6 ± 20.4 Example 6 C48 / 80 110.2 ± 18.5 Comparative Example 1 C48 / 80 141.8 ± 21.5

As can be seen in Table 5, when the pre-treatment with the composition of Examples 1 to 6 according to the present invention can be confirmed that the number of scratches is reduced to suppress the itching.

Therefore, the results of the above test for alleviating inflammation and suppressing skin itch symptoms, it can be seen that the composition according to the present invention can improve the scalp condition.

Test Example 4 Hair Loss Prevention Effect Test

Seven subjects with initial hair loss were tested for anti-hair loss for 12 weeks. After using the compositions of Examples 1 to 6 and Comparative Example 1 once a day for 12 weeks, after 12 weeks, the hair loss was collected again when the hair was collected and the number is shown in Table 6 below.

Treated group Dropout Count Example 1 62 Example 2 60 Example 3 59 Example 4 59 Example 5 60 Example 6 55 Comparative Example 1 69

As can be seen in Table 6, it was confirmed that the treatment of the compositions of Examples 1 to 6 according to the present invention significantly reduced the number of falling off hair compared to the case of treating the composition of Comparative Example 1.

Test Example 5 Hair Strength Test

Experiments were conducted on the hair strengthening effect of the compositions according to the invention.

Experimental tress hair was treated with the same amount of the composition of Examples 1 to 6 and Comparative Example 1 and dried at room temperature for 30 minutes, and hair with a thickness of 80 to 90 μm was collected from the dried experimental tress hair. The experimental tress hair was cut 10 cm, and a paper weight of a certain weight was hung on both ends, and the hook was placed on a ring 7 cm above the bottom to balance both sides, and the distance between both ends of the experimental tress hair was measured with a vernier caliper. After experimenting with 15 hairs, the average distance was calculated, and the results are shown in Table 7 below.

Treated group Hair Distance (cm) Example 1 2.50 Example 2 2.52 Example 3 2.51 Example 4 2.52 Example 5 2.53 Example 6 2.56 Comparative Example 1 2.10

As can be seen from Table 7, it can be seen that when treated with the compositions of Examples 1 to 6 according to the present invention, the hair distance is long, which has the effect of causing power to the hair.

Test Example 6 Hair Growth Effect Test

The hair growth effects of the compositions according to the invention were tested.

7 week old mice (C57BL / 6) were removed from the dorsal area of hair, and the skin of the dorsal area was selected to select 7 animals per material group, and the composition of Examples 1 to 6 and Comparative Example 1 was added to each mouse group every day. 150 μl per subject was applied for 21 days. As a negative control group, the peony extract was 0.5% by weight and dissolved in a solvent composed of water / ethanol / 1,3-butylene glycol (5/3/2).

The weight of the newly grown hair was measured and compared with the negative control group, and the results are shown in Table 8 below.

Treated group Hair weight (mg) Negative control group 26 ± 15 Comparative Example 1 60 ± 9 Example 1 76 ± 12 Example 2 78 ± 11 Example 3 79 ± 12 Example 4 76 ± 10 Example 5 77 ± 11 Example 6 80 ± 10

As can be seen in Table 8, when Examples 1 to 6 according to the present invention were applied, the average value of hair weight was higher than that of the group in which the general solution (water / ethanol / 1,3-butylene glycol) was applied. It can be confirmed that there is an excellent hair growth promoting effect by promoting the transition to the growth hair cycle by acting on the resting hair cycle mouse.

Therefore, when the hair root is strengthened, the hair is generated, hair loss is prevented, and the hair growth effect is obtained. From the test results, it can be seen that the composition according to the present invention has the effect of strengthening the hair root. In addition, it can be confirmed that there is an excellent hair growth promoting effect and excellent hair growth promoting effect.

[Test Example 7] Collagen Biosynthesis Promotion

The collagen biosynthesis-promoting effect of the mixture of volcanic and clustered ashes and cedar extracts, rape blossoms, bamboo extracts and green tea extracts was measured in comparison with tocopherol and EGCG.

First, fibroblasts were seeded at 10 ⁵ per hole in 24 wells and cultured until 90% growth. After culturing with serum-free DMEM medium for 24 hours, the volcanic rock cluster, cedar extract, rape blossom extract, bamboo extract, green tea extract, tocopherol, and EGCG dissolved in serum-free medium were treated at 10 ^-4 molarity. CO ₂ for hours Lt; / RTI &gt; These supernatants were removed and procollagen increased or decreased using procollagen type I ELISA kit (procollagen type (I)). The results are shown in Table 9, and the synthetic ability of collagen is compared to the non-treated group as 100.

division Collagen Synthesis (%) Untreated group 100 Tocopherol 113 EGCG 120 Volcanic Stone Cluster 125 Volcanic Stone Cluster + Cedar Extract (1: 1) 129 Volcanic Stone Cluster + Rape Blossom Extract (1: 1) 126.5 Volcanic Stone Cluster + Bamboo Extract (1: 1) 127 Volcanic Stone Cluster + Green Tea Extract (1: 1) 128 Volcanic Stone Cluster + Cedar Extract + Rape Flower Extract + Bamboo Extract + Green Tea Extract (1: 1: 1: 1: 1) 131

From the results of Table 9, the volcanic stone cluster according to the present invention was found to have a higher collagen synthesis capacity than the positive control group tocopherol and EGCG, volcanic stone cluster and cedar extract, rape blossom extract, bamboo extract, green tea extract or When using the mixture together it was confirmed that the improved collagen synthesis ability.

Therefore, the mixture of volcanic stone cluster and volcanic stone cluster of the present invention and cedar extract, rape blossom extract, bamboo extract or green tea extract can reduce the enlarged pores by increasing the amount of collagen production around the pores.

Test Example 8 Evaluation of Wrinkle Improvement Effect

In order to find out the effect of improving the wrinkles of Examples 1 to 6 and Comparative Example 1 was performed as follows. After dividing 70 women in their 20's and 40's into 10 groups, they applied the lotion of Examples 1 to 6 and Comparative Example 1 to the designated eye areas twice a day for 8 weeks each morning and evening, and then replicated in silicone. ) And the wrinkles at the designated sites were measured with a visometer (SVI60, Courage + Khazaka electronic GmbH, Germany). The results are shown in Table 10, which shows the average of each parameter value after 8 weeks minus the parameter value 8 weeks ago. In other words, the negative the value, the higher the wrinkle improvement effect.

division R1 R2 R3 R4 R5 Example 1 -0.31 -0.20 -0.13 -0.09 -0.08 Example 2 -0.33 -0.22 -0.16 -0.09 -0.09 Example 3 -0.32 -0.23 -0.15 -0.10 -0.10 Example 4 -0.34 -0.21 -0.17 -0.12 -0.09 Example 5 -0.35 -0.25 -0.18 -0.11 -0.11 Example 6 -0.40 -0.29 -0.25 -0.16 -0.14 Comparative Example 1 0.06 0.05 0.02 -0.01 0.00

R1: difference between the highest and lowest of the wrinkle contour

R2: The wrinkle contours are divided randomly by 5 spaces, and then the average of R1 values

R3: Highest value of R1 divided by 5

R4: Baseline of the wrinkle contour, minus the top and valley of each angle

R5: Mean value of the baseline of the wrinkle contour subtracted each wrinkle profile

As shown in Table 10, Examples 1 to 6 according to the present invention was found to be superior to the effect of improving the skin wrinkles compared to Comparative Example 1 that contains nothing.

Test Example 9 Evaluation on Skin Stability

In order to confirm the skin safety of the composition of the present invention, four adults women and four men (average 34.5 years old) were subjected to a patch test applying Examples 1 to 6 and Comparative Example 1 to the skin of the composition of the present invention The stability was evaluated.

In the measurement method, the patch was removed 28 hours after the patch was attached, and the first reading was performed after 30 minutes, and the second reading was performed after 96 hours. In order to determine the intensity of the skin irritation of the sample, the weight was given according to the degree of the positive reaction of the skin to obtain an average skin reactivity to visually determine the skin irritation of the sample. The results are shown in Table 11 below.

Test substance Average responsiveness Judgment grade Example 1 0.000 No stimulation Example 2 0.000 No stimulation Example 3 0.000 No stimulation Example 4 0.000 No stimulation Example 5 0.000 No stimulation Example 6 0.000 No stimulation Comparative Example 1 0.100 stimulus

From the results of Table 11, it can be seen that Examples 1 to 6 do not all irritate the skin.

Therefore, the cosmetic composition of this invention can be judged that it is excellent in stability to skin.

Claims (6)

Cosmetic composition for promoting hair growth containing volcanic stone cluster (Scoria) as an active ingredient. Anti-aging cosmetic composition containing volcanic stone cluster (Scoria) as an active ingredient. The composition according to any one of claims 1 to 2, wherein the composition is a Cryptomeria japonica extract, a Rassica camperstris (rapeseed) flower extract, a Bamboo (Bambusa vulgaris) extract, and a Camellia sinensis leaf extract. Cosmetic composition, characterized in that it further contains one or more selected from the group consisting of. The cosmetic composition according to claim 3, wherein the volcanic stone cluster, cedar extract, rapeseed extract, bamboo extract and green tea extract are contained in an amount of 0.0001 to 80.0 wt% based on the total weight of the composition. The cosmetic composition for promoting hair growth according to claim 1, wherein the composition is for inhibiting activity of 5α-reductase. According to claim 2, wherein the composition is a cosmetic composition, characterized in that for promoting the biosynthesis of collagen.