KR102719515B1 - Composition for Preventing Hair Loss or Promoting Hair Growth - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss and promoting hair growth, comprising at least one of a Houttuynia cordata extract, a Polygonum multiflorum Thunb extract, and a Cnidium officinale extract. The composition of the present invention promotes hair growth by inducing the growth phase of hair follicles and delaying transition to the catagen phase, and therefore has excellent effects for preventing hair loss, hair growth, and hair restoration, and has almost no side effects and excellent usability. Accordingly, a cosmetic composition for hair growth containing the composition of the present invention as an effective ingredient can be provided, and can be applied to various hair products such as hair tonics, hair shampoos, and hair conditioners.

Description

{Composition for Preventing Hair Loss or Promoting Hair Growth}

The present invention relates to a composition for preventing hair loss and/or promoting hair growth, comprising at least one of a Houttuynia cordata extract, a Polygonum multiflorum Thunb extract, and a Cnidium officinale extract.

As times change and interest in beauty increases, hair is playing an important role. Hair is a fine, keratinized structure produced on the surface of the skin. In addition to acting as a cushion against external shocks, it also protects the human body from external stimuli such as direct sunlight, cold, friction, and danger, and functions to excrete heavy metals such as arsenic, mercury, and zinc that are harmful to the body. In modern times, the aesthetic aspect of decoration is also emphasized. However, the number of people complaining of hair loss due to various reasons, including changes in diet and increased internal and external stress, is increasing. It is known that genetic factors are the main cause of hair loss, but as mentioned above, increased social stress, environmental pollution, frequent perms and dyeing, and improper scalp management are also emerging as causes of hair loss.

A human has about 100,000-150,000 hairs, and each hair has a different growth cycle. In the early stage of hair development, a placode is formed by basal epidermal cells through the action of β-catenin (Sarah E. Miller, 2002), and dermal fibroblasts that receive signals from the formed placode form a dermal condensate. At the same time, the placode grows toward the dermis, invaginates, and merges with the dermal condensate to form a primary hair germ. The epidermal cells that form the hair germ grow long in the direction of the developing dermis to form a hair peg, a long, columnar structure made of multiple layers, and the dermal condensate is formed into a spherical dermal papilla. The tip of the hair peg gradually thickens to form a hair bulb. The hair bulb surrounds the hair papilla to complete the hair follicle, and the epidermal cells inside the hair follicle differentiate to produce hair.

The first hair that is created goes through a growth phase, a degenerative phase, a resting phase, and then falls out and enters the growth phase again. The hair growth cycle is divided into three stages: the growth phase (anagen stage) in which hair grows most actively, the degenerative phase (catagen stage) in which hair begins to deteriorate, and the resting phase (telogen stage) in which hair stops growing or enters a resting state.

It is known that the activity of outer root sheath cells, including hair papilla cells and hair follicle stem cells that constitute the hair follicle, and various cytokines and growth factors produced by these cells play an important role in regulating the hair growth cycle. For example, DKK-1 (Dickkopf-1) plays an important role in hair follicle growth inhibition and death (J Invest. Dermatol, 2008:128(2)).

Meanwhile, for periodic hair growth, hair follicle regeneration and stem cell activation must occur, and this is known to be achieved through the production and activation of Wnt/β-catenin (Nature 2007:447(7142)).

In addition, growth factors such as VEGF, IGF-1, and FGF are known to promote the growth phase of hair, and TGFb-1 and EGF are known to be growth factors that induce the regression phase of hair follicles.

Alopecia generally refers to a condition in which the proportion of hair in the growth phase becomes shorter and the proportion of hair in the catagen or resting phase increases, resulting in an abnormal increase in the number of hairs that fall out. Hair loss is a normal phenomenon, but while normal people have more hair in the growth phase, people with alopecia usually have more hair in the resting phase, resulting in visible hair loss. A characteristic of people with alopecia is that their hair is miniaturized. As hair loss progresses, the period of the growth phase shortens, the transition to the catagen and resting phases is accelerated, and the volume of the subsequent hair papilla decreases, causing the hair follicles to become smaller. Therefore, in order to treat hair loss, it is important to allow hair follicles in the resting phase to quickly transition to the growth phase and to lengthen the shortened growth phase.

As mentioned earlier, the causes of hair loss have been discussed, including male hormones, excessive sebum secretion, poor blood circulation, decreased scalp function due to peroxides or bacteria, genetic factors, aging, and stress. However, the exact cause of hair loss has not yet been identified, and it is known to be a very complex intertwined process.

For such diverse and complex causes of hair loss, currently known hair loss prevention products contain ingredients for the purpose of promoting blood circulation, strengthening hair root function, moisturizing the scalp, preventing dandruff, antioxidant effect, extending the hair growth period, and suppressing androgen action, but none have shown a clear effect yet, and there are also concerns about side effects.

Currently, the only topical hair loss treatment approved by the U.S. Food and Drug Administration (FDA) is minoxidil (2,4-diamino-6-piperidinopyrimidine-3-oxide), but its effectiveness is unsatisfactory at 38% and 13% after 32 and 48 weeks of use, respectively (J Am Acad Dermatol. 2002. 47(3)), and it has the disadvantage of being less effective on the frontal hairline.

Throughout this specification, numerous references are made and cited. The disclosures of the cited references are incorporated herein by reference in their entirety so as to better explain the state of the art to which the present invention pertains and the content of the present invention.

Republic of Korea Patent Publication No. 10-2018-0110939

The inventors of the present invention have made great efforts to develop a composition that is safe for the human body and can effectively act on hair growth or hair growth promotion without the disadvantages of the conventional hair loss treatment agents, such as side effects or precautions for use, and without the weak hair growth promotion effect of hair loss prevention/hair growth promotion agents, and as a result, they have discovered that an extract of Houttuynia cordata, an extract of Polygonum multiflorum, and an extract of Cnidium officinalis have a hair growth promotion effect and that when two or more kinds of plant extracts selected from the extract of Houttuynia cordata, an extract of Polygonum multiflorum, and an extract of Cnidium officinalis are applied simultaneously, a synergistic effect is produced, thereby completing the present invention.

Therefore, the purpose of the present invention is to provide a composition for preventing hair loss or promoting hair growth.

Another object of the present invention is to provide a hair or scalp product comprising the composition for preventing hair loss or promoting hair growth.

Other objects and advantages of the present invention will become more apparent from the detailed description of the invention, the claims and the drawings which follow.

One aspect of the present invention is to provide a composition for preventing hair loss or promoting hair growth, comprising as an active ingredient at least one plant extract selected from the group consisting of Houttuynia cordata, Polygonum multiflorum Thunb, and Cnidium officinale.

Houttuynia cordata is a perennial herb of the family Trifoliaceae and is also called yakmomil, jungyakcho, jupchae, or sipyak. Houttuynia cordata contains various essential oils and has a unique smell similar to the smell of meat, hence the name houttuynia cordata. Houttuynia cordata usually blooms from May to July, and is a medicinal herb used in the private sector for gonorrhea, enteritis, urinary tract infections, pneumonia, and bronchitis.

Hasuo is a perennial herb and vine medicinal plant belonging to the family Polygonaceae. It is used as a tonic, sedative, emollient, blood tonic, and antitussive, and is known to be used in the treatment of colds, hemoptysis, neurasthenia, and arthritis.

Cheongung is a perennial herbaceous plant belonging to the family Umbelliferae, also called gungung, and is native to China and commonly cultivated in Korea. It grows upright and has long branches, reaching a height of 30–60 cm. Young shoots are eaten as greens, and the rhizomes are dried and used as medicine. Dig up the rhizomes from September to November, remove the leaves and stems, dry them in the sun, and boil them to take them, or make them into pills or powders to use.

The plant extracts selected from the group consisting of the herbaceous plant, the common ginseng and the celery root used in the present invention include all extracts including extracts using organic solvents, aqueous extracts using water, and extracts using supercritical extraction. Examples of extraction solvents used for the extraction of the herbaceous plant, the common ginseng and the celery root include purified water; lower alcohols such as methanol, ethanol, isopropyl alcohol and butanol; polyhydric alcohols such as glycerol, ethylene glycol, propylene glycol and 1,3-butylene glycol; and hydrocarbon solvents such as petroleum ether, methyl acetate, ethyl acetate, benzene, hexane, methylene chloride, diethyl ether, dichloromethane and chloroform, and one or two or more kinds of these solvents may be used in combination, but are not necessarily limited thereto.

The plant extract of the present invention may include one or more kinds of plant extracts, preferably two or more kinds of plant extracts, and most preferably three or more kinds of plant extracts. When two or more kinds are used in combination, a synergistic effect is exhibited with respect to hair loss prevention and hair growth promotion, and in particular, when three kinds of plant extracts are combined, a more remarkable synergistic effect is exhibited.

In one embodiment, the composition of the present invention may include a water parsley extract and a celery root extract, preferably a Houttuynia cordata extract, a water parsley extract and a celery root extract, and most preferably a water parsley extract, a water parsley extract and a celery root extract in a weight ratio of 6:2.5:1 to 7.5:2.5:1 (Houttuynia cordata extract: water parsley extract: celery root extract).

In another embodiment, the extract of the plant may be an extract of the plant leaves and petioles, the extract of the plant may be an extract of the plant roots, and the extract of the plant may be an extract of the plant roots.

The composition of the present invention can exhibit the effect of promoting proliferation of hair papilla cells and/or increasing hair elasticity by containing an effective amount of one or more plant extracts.

The above effective amount refers to an amount sufficient to prevent, improve, and treat hair loss. This may be appropriately changed depending on the degree of symptoms of the disease, the patient's age, weight, health condition, gender, route of administration, and treatment period.

Specifically, in the composition of the present invention, the effective amount may be 0.0001 to 50 wt% of each of the Houttuynia cordata extract, the Polygonum multiflorum Thunb extract, and the Cnidium officinale extract, and preferably 0.001 to 10.0 wt%. When the effective ingredients are included in an amount of less than 0.0001 wt%, the hair growth induction and promotion effect is minimal, and when the concentration exceeds 50 wt%, the stability of the formulation deteriorates, which is not preferable.

In one embodiment, the composition of the present invention may further comprise minoxidil, in which case the minoxidil may be comprised in an amount of 0.1 to 10.0 wt% of the total composition.

In another embodiment, the composition of the present invention may not contain a red ginseng extract. It is known that existing compositions for preventing hair loss and promoting hair growth, which contain a mixture of herbal extracts, contain red ginseng extract as an essential ingredient. However, red ginseng extract is expensive and may cause side effects such as fever, palpitations, and insomnia when administered for a long period of time. On the other hand, the composition according to one embodiment of the present invention is characterized by having excellent hair loss prevention and hair growth promotion effects, despite not containing a red ginseng extract.

It is preferable to use the composition for preventing hair loss or promoting hair growth of the present invention by a percutaneous administration method such as directly applying it to the skin or spraying it.

The term "administration" used in the present invention means introducing the composition of the present invention by any appropriate method, and the route of administration of the composition of the present invention may be administered by any general route as long as it can reach the target tissue. Preferably, it may be administered by transdermal administration, and among them, topical application is most preferred. The number of times the composition of the present invention is applied may be determined according to prescription, need, or desire.

The amount of the composition of the present invention may be appropriately adjusted depending on individual differences such as age, degree of lesion, etc., and the formulation, and it is usually preferable to apply an appropriate amount to the scalp once or several times a day and use it for one week to several months. In one embodiment of the present invention, Composition 1 for hair loss treatment was used five times a week for six months, and as a result, it was confirmed that the hair growth promotion effect was superior to that of the control group, minoxidil alone, in preventing hair loss (Table 6).

As one embodiment of the present invention, the composition may be a pharmaceutical composition for preventing or treating hair loss. In this case, the composition for preventing hair loss or promoting hair growth may include one or more pharmaceutically acceptable carriers, excipients or diluents together with one or more of the plant extracts described above.

The term “pharmaceutically acceptable” as used herein refers to a composition that is physiologically acceptable and does not typically cause allergic reactions such as gastrointestinal upset, dizziness, or similar reactions when administered to humans.

Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, polyvinylpyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate and mineral oil. In addition, fillers, anticoagulants, lubricants, wetting agents, fragrances, emulsifiers and preservatives may be additionally included.

In another aspect of the present invention, the composition may be a composition for quasi-drugs. In this case, the effective ingredient of the present invention may be added as is or used together with other quasi-drug ingredients, and may be appropriately used according to a conventional method. These other ingredients may be arbitrarily selected and mixed according to the formulation or purpose of use of the quasi-drug. For example, it may include conventional excipients such as thickeners, stabilizers, solubilizers, vitamins, pigments, and fragrances, and carriers.

As another aspect of the present invention, the composition may be a cosmetic composition for preventing or improving hair loss or promoting hair growth.

The cosmetic composition of the present invention can be formulated in various forms of cosmetics known in the art, and additional ingredients can be arbitrarily selected and appropriately mixed depending on the formulation and purpose of use. For example, it can include conventional ingredients such as thickeners, surfactants, moisturizers, abrasives, sweeteners, pH adjusters, preservatives, binders, humectants, foaming agents, stabilizers, solubilizers, vitamins, pigments, and fragrances.

In addition, the present invention provides a hair or scalp product comprising the aforementioned hair loss prevention or hair growth promotion composition. The product according to the present invention comprising one or more of the aforementioned plant extracts as an effective ingredient generally encompasses products manufactured and sold for scalp health and hair growth promotion, and there is no limitation on the hair or scalp product to which it is applied.

The hair or scalp product of the present invention may be formulated in any form that can be applied to the skin, but is preferably formulated in the form of an external preparation. For example, it may be manufactured in a formulation that can be applied to the skin, such as a liquid, cream, paste, or solid formulation, and may be manufactured as a composition of a shampoo, tonic, hair conditioner, hair lotion, gel, pack, cream, essence, powder, spray, oil, soap, liquid cleanser, liquid hair tonic type, etc. for promoting hair growth by adding a conventional additive, and may be used in various ways in the form of an aerosol type of these formulations, but is not limited thereto.

When the product of the present invention is liquid, a solvent, a solubilizer or an emulsifier is used as a carrier component, and for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan can be used.

When the product of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide can be used as a carrier component.

When the product of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used as a carrier component, and particularly in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether can be additionally included.

The components included in the composition of the present invention include at least one plant extract selected from an extract of mugwort, an extract of water parsley, and an extract of angelica tree, as well as components commonly used in external preparations that can be applied to the skin, and may further include at least one additive selected from the group consisting of water, a surfactant, a moisturizer, a lower alcohol, a chelating agent, a bactericide, an antioxidant, a preservative, a pigment, and a fragrance.

The composition of the present invention as described above can promote proliferation of hair papilla cells, inhibit degeneration of hair papilla cells, and also increase thickness, density, and elasticity of hair.

The composition of the present invention contains at least one of an extract of mugwort, an extract of polygonum multiflorum, and an extract of angelica tree, and exhibits a higher effect than when minoxidil is used alone, and when two or more ingredients are used simultaneously, the composition promotes hair growth through the synergistic effect of the ingredients and inhibits transition to the catagen phase, thereby providing a hair loss treatment composition having excellent effects of preventing hair loss and promoting hair growth, thereby complementing the disadvantage of low expected effects when minoxidil is used alone.

Hereinafter, the present invention will be described in more detail through examples. These examples are only intended to explain the present invention more specifically, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples.

Example

I. Preparation of extracts

1. Preparation of extract of Eoseongcho

Houttuynia cordata ) were crushed, and an extraction solvent consisting of 50% ethanol and water in an amount 20 times the volume of the crushed material was added, and extraction was performed three times for 3 hours each time, and the extract was concentrated to obtain a dried product.

2. Preparation of the extract of the sewage sludge

Polygonum multiflorum After crushing the root of the plant (Prunella vulgaris L. Thunb. ), an extraction solvent consisting of 50% ethanol and water in a volume 20 times that of the crushed material was added, extraction was performed three times for 3 hours each time, and the extract was concentrated to obtain a dried product.

3. Preparation of Cheongung extract

Cnidium officinale ) was crushed, and an extraction solvent consisting of 50% ethanol and water in an amount 20 times the volume of the crushed material was added, and extraction was performed three times for 3 hours each time, and the extract was concentrated to obtain a dried product.

II. Experimental example

1. Effect of extracts of Eoseongcho, Hwasuo, and Cheongung on the proliferation of maternal papilla cells

Human dermal papilla cells (hDPCs) were purchased from PromoCell. The hDPCs were cultured in a culture medium (Follicle Dermal Papilla Cell Growth Medium; PromoCell, Heidelberg, Germany) containing culture medium additives (Supplement Mix; PromoCell, Heidelberg, Germany), 100 units/mL of penicillin, and 100 μg/mL of streptomycin at 37°C and 5% _CO2 . The cultured hDPCs (3.0 × 10 ³ cells/well) were dispensed into 96-well plates and cultured for 24 h. Afterwards, the cultured cells were treated with DMSO (vehicle) diluted 1:1000 in DMEM containing 1% fetal bovine serum (FBS; Gibco, NY, USA), 100 units/mL of penicillin, 100 μg/mL of streptomycin, and 1 ng/mL bFGF as a control group, 0.1 μg/mL of minoxidil as a positive control, and each of the extracts of Houttuynia cordata, Polygonum multiflorum, and Cnidium officinalis at a concentration of 10 μg/mL and incubated. In addition, the group treated simultaneously with 5 ㎍/㎖ of the extract of Houttuynia cordata and 5 ㎍/㎖ of the extract of Polygonum multiflorum, the group treated simultaneously with 5 ㎍/㎖ of the extract of Polygonum multiflorum and 5 ㎍/㎖ of the extract of Cnidium officinalis, the group treated simultaneously with 5 ㎍/㎖ of the extract of Polygonum multiflorum and 5 ㎍/㎖ of the extract of Cnidium officinalis, and the group treated simultaneously with 3.33 ㎍/㎖ of the extract of Polygonum multiflorum and 3.33 ㎍/㎖ of the extract of Cnidium officinalis were incubated for 24 hours, and the degree of cell proliferation was evaluated using the CCK method.

And the total concentration of the extracts to be processed was fixed at 10 ㎍/㎖, and the composition ratio of the three extracts was changed, such as combinations of 2:1:1, 1:2:1, 1:1:2, and combinations of 2.5:1.25:1, 6:2.5:1, 7.5:2.5:1 with a higher ratio of the Houttuynia cordata extract, and 1:30:10, 1:30:15 with a higher ratio of the Houttuynia cordata extract and the Cnidium officinalis extract, and then incubated for 24 hours (Houttuynia cordata extract: Cnidium officinalis extract: Cnidium officinalis extract)

CCK-8 was treated with the culture medium at a ratio of 1:10 and incubated for 1 hour. After 1 hour, the absorbance of each well was measured at 450 nm. All experiments were repeated three times and the average absorbance values were calculated. The results were expressed as a percentage of the treatment group compared to the control group, which was set at 100.

As a result, the effects of proliferating mammary gland cells were confirmed in each of the extracts of Eoseongcho, Hwasuo, and Cheongung, and when two or more of each extract were treated simultaneously, a higher mammary gland cells proliferation effect was observed due to the synergistic effect (Table 1).

And when the three types were treated together, there was a difference in the effect of proliferation of mammary gland cells depending on the ratio, and the highest effect of proliferation of mammary gland cells was observed when the ratio of Eoseongcho extract: Polygonum multiflorum extract: Cnidium officinalis extract = 6: 2.5: 1 was treated.

number Treatment group Treatment concentration Breast papilla cell proliferation capacity (%) 1 Untreated group - 100 2 Minoxidil 0.1 ㎍/㎖ 127 3 Eoseongcho extract 10 ㎍/㎖ 131 4 Hasuo extract 10 ㎍/㎖ 128 5 Extract of the Heavenly Palace 10 ㎍/㎖ 126 6 Eoseongcho extract + Hwasuo extract 5 ㎍/㎖ + 5 ㎍/㎖ 145 7 Hasuo extract + Cheongung extract 5 ㎍/㎖ + 5 ㎍/㎖ 152 8 Eoseongcho extract + Cheongung extract 5 ㎍/㎖ + 5 ㎍/㎖ 149 9 Eoseongcho extract + Hwasuo extract + Cheongung extract (1:1:1) 3.33 ㎍/㎖ + 3.33 ㎍/㎖ + 3.33 ㎍/㎖ 163 10 Mugwort extract + Polygonum multiflorum extract + Cnidium officinalis extract (2:1:1) 5 ㎍/㎖ + 2.5 ㎍/㎖ + 2.5 ㎍/㎖ 168 11 Eoseongcho extract + Hwasuo extract + Cheongung extract (1:2:1) 2.5 ㎍/㎖ + 5 ㎍/㎖ + 2.5 ㎍/㎖ 160 12 Mugwort extract + Polygonum multiflorum extract + Cnidium officinalis extract (1:1:2) 2.5 ㎍/㎖ + 2.5 ㎍/㎖ + 5 ㎍/㎖ 159 13 Eoseongcho extract + Hwasuo extract + Cheongung extract (2.5:1.25:1) 5.26 ㎍/㎖ + 2.63 ㎍/㎖ + 2.1 ㎍/㎖ 170 14 Mugwort extract + Polygonum multiflorum extract + Cnidium officinalis extract (6:2.5:1) 6.32 ㎍/㎖ + 2.63 ㎍/㎖ + 1.05 ㎍/㎖ 176 15 Eoseongcho extract + Hwasuo extract + Cheongung extract (7.5:2.5:1) 6.82 ㎍/㎖ + 2.27 ㎍/㎖ + 0.91 ㎍/㎖ 171 16 Eoseongcho extract + Hwasuo extract + Cheongung extract (1:30:15) 0.22 ㎍/㎖ + 6.52 ㎍/㎖ + 3.26 ㎍/㎖ 164 17 Eoseongcho extract + Hwasuo extract + Cheongung extract (1:30:10) 0.24 ㎍/㎖ + 7.32 ㎍/㎖ + 2.44 ㎍/㎖ 167

2. Effect of extracts of Houttuynia cordata , Polygonum multiflorum, and Cnidium officinalis on the inhibition of the catagen phase of hair papilla cells It is generally known that DKK-1, secreted from hair papilla cells, induces the catagen phase of hair. Therefore, the effects of the extracts of Houttuynia cordata, Polygonum multiflorum, and Cnidium officinalis and compositions containing two or more of the extracts of the present invention on the expression of the DKK-1 gene, which is related to the catagen phase induction signal in hair papilla cells, were investigated.

After seeding 1 x 105 of mammary papilla cells in each well of a culture 6-well plate, the cells were treated with the extract of the present invention, the extract of Polygonum multiflorum, and the extract of Cnidium officinalis at a concentration of 10 μg/mL each. The groups treated simultaneously with 5 μg/mL of the extract of Polygonum multiflorum and 5 μg/mL of the extract of Polygonum multiflorum, 5 μg/mL of the extract of Polygonum multiflorum and 5 μg/mL of the extract of Cnidium officinalis, 5 μg/mL of the extract of Polygonum multiflorum and 5 μg/mL of the extract of Cnidium officinalis, and 3.33 μg/mL of the extract of Polygonum multiflorum and 3.33 μg/mL of the extract of Cnidium officinalis were incubated for 24 hours.

And the total concentration of the extracts to be processed was fixed at 10 ㎍/㎖, and the composition ratio of the three extracts was changed, such as combinations of 2:1:1, 1:2:1, 1:1:2, and combinations of 2.5:1.25:1, 6:2.5:1, 7.5:2.5:1 with a higher ratio of the Houttuynia cordata extract, and 1:30:10, 1:30:15 with a higher ratio of the Houttuynia cordata extract and the Cnidium officinalis extract, and then incubated for 24 hours (Houttuynia cordata extract: Cnidium officinalis extract: Cnidium officinalis extract)

After incubation, cells for each treatment group were obtained, and then the expression level of DKK-1 was examined using the real-time PCR technique using Taqman® Probe (Thermo Fisher, Massachusetts, USA) in [Table 2] below, and the results are shown in [Table 3].

gene Taqman ^ⓡ Probe Assay ID DKK-1 Hs00183740_m1

As a result, the inhibitory effects of the extracts of Houttuynia cordata, Polygonum multiflorum, and Cnidium officinalis in the gene expression of DKK-1 in maternal papilla cells were confirmed, and when two or more of each extract were treated simultaneously, a higher inhibitory effect was confirmed due to the synergistic effect (Table 3). In addition, when three types were treated together, there were differences in the proliferation effects of maternal papilla cells depending on the ratio, and the highest DKK-1 inhibitory effect was observed when the ratio of Houttuynia cordata extract: Polygonum multiflorum extract: Cnidium officinalis extract = 7.5: 2.5: 1 was treated.

number Treatment group Treatment concentration DKK-1 expression level (%) 1 Untreated group - 100 2 Minoxidil 0.1 ㎍/㎖ 126 3 Eoseongcho extract 10 ㎍/㎖ 81 4 Hasuo extract 10 ㎍/㎖ 77 5 Extract of the Heavenly Palace 10 ㎍/㎖ 79 6 Eoseongcho extract + Hwasuo extract 5 ㎍/㎖ + 5 ㎍/㎖ 74 7 Hasuo extract + Cheongung extract 5 ㎍/㎖ + 5 ㎍/㎖ 71 8 Eoseongcho extract + Cheongung extract 5 ㎍/㎖ + 5 ㎍/㎖ 76 9 Eoseongcho extract + Hwasuo extract + Cheongung extract (1:1:1) 3.33 ㎍/㎖ + 3.33 ㎍/㎖ + 3.33 ㎍/㎖ 67 10 Mugwort extract + Polygonum multiflorum extract + Cnidium officinalis extract (2:1:1) 5 ㎍/㎖ + 2.5 ㎍/㎖ + 2.5 ㎍/㎖ 62 11 Eoseongcho extract + Hwasuo extract + Cheongung extract (1:2:1) 2.5 ㎍/㎖ + 5 ㎍/㎖ + 2.5 ㎍/㎖ 66 12 Mugwort extract + Polygonum multiflorum extract + Cnidium officinalis extract (1:1:2) 2.5 ㎍/㎖ + 2.5 ㎍/㎖ + 5 ㎍/㎖ 68 13 Eoseongcho extract + Hwasuo extract + Cheongung extract (2.5:1.25:1) 5.26 ㎍/㎖ + 2.63 ㎍/㎖ + 2.1 ㎍/㎖ 63 14 Mugwort extract + Polygonum multiflorum extract + Cnidium officinalis extract (6:2.5:1) 6.32 ㎍/㎖ + 2.63 ㎍/㎖ + 1.05 ㎍/㎖ 64 15 Eoseongcho extract + Hwasuo extract + Cheongung extract (7.5:2.5:1) 6.82 ㎍/㎖ + 2.27 ㎍/㎖ + 0.91 ㎍/㎖ 60 16 Eoseongcho extract + Hwasuo extract + Cheongung extract (1:30:15) 0.22 ㎍/㎖ + 6.52 ㎍/㎖ + 3.26 ㎍/㎖ 69 17 Eoseongcho extract + Hwasuo extract + Cheongung extract (1:30:10) 0.24 ㎍/㎖ + 7.32 ㎍/㎖ + 2.44 ㎍/㎖ 68

As described above, the hair growth induction and hair loss inhibition effects of the extracts of Eoseongcho, Polygonum multiflorum, and Cnidium officinalis were confirmed, and when two or more of the extracts were treated simultaneously, a higher hair growth and hair loss inhibition effect was observed due to the synergistic effect. Accordingly, the composition for preventing hair loss and promoting hair growth according to the present invention containing the extracts was prepared in various formulations. The formulation examples are described in detail below. However, it will be apparent to those skilled in the art that these formulation examples are only examples of the composition for promoting hair growth of the present invention, and the scope of the composition of the present invention is not limited to these formulations.

3. Composition for hair growth Hair growth effect Verification test

(1) Composition for hair loss treatment 1 (Hair tonic)

Hair tonic was prepared using extracts of mugwort, water parsley, and angelica tree using a conventional method according to the prescription shown in Table 4 below.

Mixing ingredients Weight ratio (%) Comparative Example 1 Comparative Example 2 Example 3 Example 4 Example 5 Example 6 Ethanol 55 55 55 55 55 55 Castor oil 5 5 5 5 5 5 glycerin 3 3 3 3 3 3 Eoseongcho extract 0 0 10 3.33 6.32 6.82 Hasuo extract 0 0 0 3.33 2.63 2.27 Extract of the Heavenly Palace 0 0 0 3.33 1.05 0.91 Minoxidil 0 5 0 0 0 0 Flavors and Colors Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Purified water Remaining amount (total 100)

(2) Composition 2 for hair loss treatment (hair lotion) A hair lotion was prepared using extracts of mugwort, isoflavones, and angelica tree in a conventional manner according to the prescription shown in Table 5 below.

Mixing ingredients Weight ratio (%) Cetostearyl alcohol 2 Stearyl triethylammonium chloride 2 Hydroxyethyl cellulose 0.5 Eoseongcho extract 3.3 Hasuo extract 3.3 Extract of the Heavenly Palace 3.3 Flavors and Colors Appropriate amount Purified water Remaining amount (total 100)

(3) Test for confirmation of hair growth effect of the hair growth composition 1 (hair tonic) The hair loss treatment composition 1 (hair tonic) of the present invention was divided into 6 groups (10 people each) and 6 groups used Comparative Example 1, Comparative Example 2, Example 3, Example 4, Example 5, and Example 6 on the hair and scalp 5 times a week for 6 months. After use, the degree of improvement (very improved; +3, improved; +2, slightly improved; +1, no change; 0, slightly worse; -1, worse; -2, very worse; -3) in terms of hair thickness, density, elasticity, and overall evaluation items was determined as the average value after 6 months by questionnaire to the users, and the results are shown in Table 6.

Sample Hair thickness density Elasticity Overall Rating Comparative Example 1 -0.5±0.3 0.2±0.4 -0.1±0.3 -0.21±0.3 Comparative Example 2 1.7±0.2 1.3±0.1 2.0±0.3 1.7±0.2 Example 3 1.9±0.3 1.7±0.1 2.1±0.4 1.9±0.1 Example 4 2.5±0.1 2.0±0.4 2.1±0.1 2.2±0.1 Example 5 2.9±0.3 2.3±0.1 2.6±0.3 2.6±0.1 Example 6 2.8±0.4 2.4±0.2 2.5±0.3 2.6±0.3

As can be seen from the results, when minoxidil (Comparative Example 2) and Houttuynia cordata extract (Example 3) were used alone, the effect was better than that of Comparative Example 1, and when Houttuynia cordata extract, Polygonum multiflorum extract, and Cnidium officinalis extract were used simultaneously in a ratio of 1:1:1 (Example 4), a synergistic effect was observed. In addition, when Houttuynia cordata extract, Polygonum multiflorum extract, and Cnidium officinalis extract were used simultaneously in ratios of 6:2.5:1 and 7.5:2.5:1 (Examples 5 and 6), a higher hair loss prevention and hair growth effect was observed due to the synergistic effect of the materials, proving that the present invention can be used very effectively for hair loss treatment.

Claims (17)

A composition for preventing hair loss or promoting hair growth, comprising plant extracts including an extract of Houttuynia cordata, an extract of Polygonum multiflorum Thunb, and an extract of Cnidium officinale as active ingredients. delete delete delete A composition according to claim 1, wherein the weight ratio of the extract of Eoseongcho, the extract of Polygonum multiflorum, and the extract of Cheon-gung is 6:2.5:1 to 7.5:2.5:1 (Eoseongcho extract: Polygonum multiflorum extract: Cheon-gung extract). A composition according to claim 1, wherein the plant extract is a purified water extract; an organic solvent extract selected from a lower alcohol having 1 to 4 carbon atoms, glycerol, ethylene glycol, propylene glycol, 1,3-butylene glycol, petroleum ether, methyl acetate, ethyl acetate, benzene, hexane, methylene chloride, diethyl ether, dichloromethane and chloroform; or a supercritical extract. A composition characterized in that in claim 1, the extract of Houttuynia cordata is an extract of Houttuynia cordata leaves and petioles, the extract of Polygonum multiflorum is an extract of Polygonum multiflorum root, and the extract of Cnidium officinalis is an extract of Cnidium officinalis root. A composition characterized in that in claim 1, the content of the effective ingredient is 0.0001 to 50 wt%. A composition according to claim 1, characterized in that the composition further comprises minoxidil. A composition, characterized in that in claim 9, the content of minoxidil is 0.1 to 10.0 wt%. In claim 1, the composition is characterized in that it promotes proliferation of mammary papilla cells and inhibits degeneration of mammary papilla cells. In claim 1, the composition is characterized in that it increases the thickness, density and elasticity of hair. A composition according to any one of claims 1 and 5 to 12, characterized in that the composition is a pharmaceutical composition, a composition for quasi-drugs, or a cosmetic composition. delete delete A hair or scalp product comprising a composition according to any one of claims 1 and 5 to 12. A product characterized in that in claim 16, the hair or scalp product is selected from the group consisting of hair tonics, scalp clinic agents, scalp scaling agents, scalp massage agents, scalp care agents, cleansers, shampoos, tonics, hair conditioners, hair lotions, gels, packs, creams, essences, powders, sprays, oils, soaps, ointments, hair styling agents, hair dyes, and perm agents.