KR102675966B1 - Composition for preventing hair loss and promoting hair growth - Google Patents

Abstract

The present disclosure relates to a composition for preventing hair loss and promoting hair growth containing an ingredient that promotes hair growth. The composition for preventing hair loss and promoting hair growth of the present disclosure promotes the activity of dermal papilla cells, the mitochondrial activity of dermal papilla cells, and the activity of the Wnt/β-catenin signaling system through a combination of specific active ingredients, thereby exhibiting significant anti-hair loss and hair growth promoting effects. It creates a synergistic effect.

Description

Composition for preventing hair loss and promoting hair growth}

The present invention relates to a composition for preventing hair loss and promoting hair growth.

As times change, hair plays an important part in the growing interest in beauty. Hair is a thin, keratinized structure produced on the surface of the skin. In addition to acting as a cushion against external shock, it protects the human body from external stimuli such as direct sunlight, cold, friction, and danger, and has the function of excreting heavy metals such as arsenic, mercury, and zinc, which are harmful to the body, out of the body. In modern times, it is used as a decorative beauty. Aspects are also emphasized. However, the number of people complaining of hair loss due to various causes, including changes in diet or increased internal and external stress, is increasing. It is known that genetic factors are the main cause of hair loss, but as mentioned above, increased social stress, environmental pollution, frequent perms and dyeing, and incorrect scalp management are also emerging as causes of hair loss.

There are approximately 100,000 to 150,000 human hairs, and each hair has a different growth cycle. In the early stages of hair follicle formation, a placode is formed by basal layer epidermal cells through the action of β-catenin (Sarah E. Miller, 2002). Dermal fibroblasts that receive signals from the formed placode form dermal condensates. At the same time, the placode grows and invaginates toward the dermis and merges with skin condensates to form primary hair germ. The epidermal cells that form the hair germ grow long in the direction of the developing dermis, forming hair pegs, which are long column-shaped structures made of multiple layers, while skin condensates form spherical dermal papilla. do. The end of the hair peg gradually becomes thicker to form a hair bulb. The hair bulb wraps around the dermal papilla to form a hair follicle, and the epidermal cells inside the hair follicle differentiate to produce hair.

The hair that is first created goes through the growth phase, then into the involution phase and then into the resting phase, and then the hair falls out and goes back into the growth phase again. The hair growth cycle consists of three stages: the anagen stage, when hair grows most actively, the catagen stage, when hair begins to deteriorate, and the telogen stage, when hair growth stops or enters rest. ) is classified as.

In regulating this hair growth cycle, the activity of outer root sheath cells, including dermal papilla cells and hair follicle stem cells that make up hair follicles, and various cytokines and growth factors produced by these cells play an important role. It is known that it does. For example, DKK-1 (Dickkopf-1) plays an important role in hair follicle growth inhibition and death. Meanwhile, for periodic hair growth, hair follicle regeneration and stem cell activation must occur, and this is known to be achieved through the production and activation of Wnt/β-catenin. In addition, growth factors such as VEGF, IGF-1, and FGF are known to promote the growth phase of hair, and TGFb-1 and EGF are known to be growth factors that induce the catagen phase of hair follicles.

In general, alopecia refers to a condition in which the proportion of hair in the growth phase becomes shorter and the number of hair in the catagen or telogen phase increases during this cycle, resulting in an abnormal increase in the number of hair shedding. Hair loss is a normal phenomenon, but while normal people have a lot of hair in the growth phase, people with alopecia usually have a lot of hair in the telogen phase, resulting in visible hair loss. A characteristic feature of people with alopecia is miniaturization of hair. As hair loss progresses, the growth period becomes shorter, the transition to the catagen and telogen stages is accelerated, and the subsequent volume of the dermal papilla decreases, causing the hair follicles to become increasingly smaller. Therefore, in order to treat hair loss, it is important to allow hair follicles in the resting state to quickly transition to the growth phase and to lengthen the shortened growth phase.

As mentioned earlier, the causes of hair loss have been discussed as the effects of male hormones, excessive sebum secretion, poor blood circulation, decreased scalp function due to peroxides or bacteria, genetic factors, aging, and stress. However, to date, the clear cause of hair loss has not been revealed, and it is known to be a very complex process.

In response to these diverse and complex causes of hair loss, hair loss prevention products known to date include active ingredients that promote blood circulation, strengthen hair root function, scalp moisturizing effect, anti-dandruff effect, antioxidant effect, hair growth extension effect, and male hormone effect inhibition. Although it contains the intended ingredients, there is still no one with a clear effect in terms of effectiveness, and the problem of side effects is sometimes raised.

Meanwhile, adenosine is an amino acid made in cells and is a main component of cellular energy metabolism. Adenosine is one of the elements that make up DNA, a gene, and is a compound made up of a base called adenine and a sugar called ribose. When three phosphates combine with a sugar, adenosine becomes ATP (adenosine 5'-triphosphate), the main source of intracellular energy. Accordingly, adenosine is known to be an important element that plays a decisive role in the smooth progress of all metabolism in the body. Because adenosine is an intracellular component, it has excellent stability and durability when penetrating the skin, and has a synergistic effect in improving skin wrinkles by promoting DNA and protein synthesis in the dermal layer.

In addition, niacinamide is a type of vitamin B complex and is widely distributed in the plant world, coexisting with nicotinic acid. In the animal world, it exists as a component of NAD or NADP, a redox coenzyme. Niacinamide is a bioactive amide of vitamin B3, also known as pyridine-3-carboxylic acid, and is known to act as an anti-inflammatory ingredient or antioxidant. Niacinamide inhibits the movement of melanosomes, thereby inhibiting the movement of synthesized melanin to keratinocytes, thereby reducing skin pigmentation and increasing whitening effect. In addition, niacinamide has a beneficial effect on skin moisturization by increasing collagen synthesis and intercellular lipid synthesis.

Additionally, D-panthenol (dexpanthenol) is an alcoholic form of vitamin B5 (Pantothenic acid) and can be called provitamin B5. D-Panthenol is absorbed into the skin and converted directly into vitamin B5. D-Panthenol is widely used in cosmetics and household goods as an ingredient in skin moisturizers and wrinkle improvers, and is also used as an ointment for wound healing, skin burns, and itchiness improvement. In addition, D-panthenol is known as a penetrating moisturizer, and is known to have a conditioning effect on skin and hair and increase recovery of damaged hair.

In addition, Hasuo is a perennial herbaceous vine medicinal plant belonging to the knotweed family, and its scientific names are Pleuropterus multiflorus , Polygonum multiflorum , Fagopyrum multiflorum , Reynoutria multiflora , or Fallopia multiflora . The extract of Haushuo is used as a tonic, tonic, tonic, blood purifier, or antitussive, and is known to be used to treat colds, hematemesis, nervous breakdown, or arthritis.

In addition, Houttuynia cordata is a perennial herb of the Trifoliaceae family and is a medicinal herb known as medicinal herb, Chinese herb, japchae, or ten yak. Houttuynia cordata is called Houttuynia cordata because it contains a variety of essential oils and produces a unique smell like fishy meat. Houttuynia cordata mainly blooms from May to July, and is a medicinal herb used in the private sector for gonorrhea, enteritis, urinary tract infection, pneumonia, or bronchitis.

Additionally, zinc pyrithione has an antibacterial effect and prevents the growth of microorganisms on the scalp and skin. In particular, zinc pyrithione has an inhibitory effect on the growth of malassezia and other fungi, preventing seborrheic dermatitis and dandruff on the scalp.

In addition, salicylic acid is the main component of aspirin and has been widely used as an anti-inflammatory, analgesic, or antipyretic agent. Salicylic acid has the effect of exfoliating and softening the skin. Salicylic acid is mainly added to cosmetics such as cleansing foam to soften pores by softening and exfoliating dead skin cells. Through this, salicylic acid can help excrete sebum from pores and keep the skin clean. In addition, salicylic acid exhibits antibacterial and anti-inflammatory effects and can improve the skin and scalp or prevent dandruff by inhibiting the growth of scalp or skin microorganisms.

Meanwhile, the only topical hair loss treatment currently approved by the U.S. Food and Drug Administration (FDA) is minoxidil (2,4-diamino-6-piperidinopyrimidine-3-oxide), but its effectiveness is 38% when used for 32 to 48 weeks. and 13%, which is unsatisfactory, and has the disadvantage of being less effective on bangs.

As such, there is a need to develop a composition that is effective in preventing hair loss or promoting hair growth. When adenosine, D-panthenol, niacinamide, Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid are combined, hair loss prevention or There is a lack of research on what effect it has on hair growth promotion.

The problem to be solved by the present disclosure is to provide a composition for preventing hair loss or promoting hair growth that has excellent hair loss preventing and/or hair growth promoting effects.

In addition, the problem to be solved by the present disclosure is to provide a hair or scalp product containing the composition for preventing hair loss or promoting hair growth.

The present inventors have improved problems such as side effects and precautions for use of conventional hair loss treatments, and have developed a method that is safe for the human body and can effectively prevent hair loss and promote hair growth without disadvantages such as the weak hair growth promotion effect of hair loss prevention/hair growth agents. We made diligent efforts to develop a composition that contains As a result, the present inventors discovered that when specific active ingredients are additionally combined with a mixture of adenosine, D-panthenol, and niacinamide, a significant synergistic effect occurs in preventing hair loss and promoting hair growth.

Specifically, through in-vitro experiments, the present inventors added at least one selected from the group consisting of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid to a mixture of adenosine, D-panthenol, and niacinamide. When combined, there is a significant synergy effect in the effect of promoting hair papilla cell activity (Experimental Example 1), the effect of promoting mitochondrial activity of hair papilla cells (Experimental Example 2), and the effect of promoting dermal papilla cell activity signaling (Wnt/β-catenin) (Experimental Example 3). It was confirmed that this occurred. In addition, the present inventors confirmed that when treating alopecia patients with a composition for preventing hair loss or promoting hair growth containing the above combination, the number of hairs per unit area and the average hair thickness were significantly improved, resulting in a significantly excellent effect in the treatment of alopecia. (Experimental Examples 4 and 5).

The present disclosure provides a mixture of adenosine, D-panthenol, and niacinamide; And it provides a composition for preventing hair loss or promoting hair growth, comprising as an active ingredient at least one selected from the group consisting of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid. In one aspect of the disclosure, the disclosure provides a mixture of adenosine, D-panthenol, and niacinamide; Houttuynia cordata extract, Houttuynia cordata extract, or both; And it provides a composition for preventing hair loss or promoting hair growth containing zinc pyrithione, salicylic acid, or both as active ingredients. The composition for preventing hair loss or promoting hair growth produces a significant synergy effect in preventing hair loss or promoting hair growth by combining the active ingredients.

In one aspect of the present disclosure, the Houttuynia cordata extract or Houttuynia cordata extract can be extracted by a method known in the art, and the method is not particularly limited, or a commercially available extract can be used. In another embodiment, the extract of Houttuynia cordata or Houttuynia cordata extract may be an extract obtained by extracting Houttuynia cordata or Houttuynia cordata with water and/or an organic solvent, and the organic solvent may be a polar organic solvent, a non-polar organic solvent, or a mixed solvent thereof. there is. The polar organic solvent may be a lower alcohol having 1 to 5 carbon atoms, ethyl acetate, or acetone, and the non-polar organic solvent may be ether, chloroform, benzene, nucleic acid, or dichloromethane. For example, the lower alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol, or isopropanol.

In one aspect of the present disclosure, the Houttuynia cordata extract or Houttuynia cordata extract may include a fraction obtained by fractionating the primary extract extracted using the above-described extraction solvent using an extraction solvent of different polarity. For example, the extract of Houttuynia cordata or Houttuynia cordata extract may be a fraction extracted with an alcohol having 1 to 5 carbon atoms and then fractionated again with a solvent of different polarity such as ether, benzene, or hexane. Two or more types of solvents can be used during the fractionation, and extracts for each solvent can be prepared by using them sequentially or by mixing them depending on the polarity of the solvents.

In one aspect of the present disclosure, the prepared extract or the fraction obtained by performing the fractionation process may be filtered, concentrated, or dried to remove the solvent, and the filtration, concentration, and drying may all be performed. there is. Specifically, the filtration can be performed using filter paper or a vacuum filter, the concentration can be performed under reduced pressure using a vacuum concentrator, etc., and the drying can be performed using a freeze-drying method. In addition, the prepared extract or the fraction obtained by performing the above fractionation process is purified using various chromatographies such as silica gel column chromatography, thin layer chromatography, or high-performance liquid chromatography to obtain an additional purified fraction. .

In one aspect of the present disclosure, a mixture of adenosine, D-panthenol, and niacinamide included in the composition for preventing hair loss or promoting hair growth of the present disclosure; And the content of the active ingredient containing any one or more selected from the group consisting of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid is 0.0001 to 10% by weight, preferably 0.001 to 7% by weight, more preferably 0.001 to 7% by weight, based on the total weight of the composition. It may be 0.01 to 5% by weight. If the total content of the active ingredients is less than 0.0001% by weight based on the total weight of the composition, the hair growth inducing promoting effect is minimal, and if it is more than 10% by weight, the stability of the formulation is poor and thus undesirable. In the present disclosure, it was confirmed through experiments that the efficacy of preventing hair loss and promoting hair growth was significantly superior when the active ingredient was in the above content range.

In one aspect of the present disclosure, the content of the mixture of adenosine, D-panthenol, and niacinamide included as active ingredients in the composition for preventing hair loss or promoting hair growth of the present disclosure is 0.0001 to 10% by weight, preferably 0.001 to 0.001% by weight, based on the total weight of the composition. It may be 10% by weight. If the content of the mixture of adenosine, D-panthenol and niacinamide is less than 0.0001% by weight based on the total weight of the composition, the effect of promoting hair growth induction is minimal, and if it is more than 10% by weight, the stability of the formulation is poor and thus undesirable.

In one aspect of the present disclosure, the content of one or more selected from the group consisting of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid included as an additional active ingredient in the composition for preventing hair loss or promoting hair growth of the present disclosure is relative to the total weight of the composition. It may be 0.00001 to 10% by weight, preferably 0.0001 to 5% by weight. If the content of any one or more selected from the group consisting of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid is less than 0.00001% by weight of the total weight of the composition, the synergistic effect in promoting hair growth is minimal, and if it is more than 10% by weight, the formulation It is undesirable due to poor stability.

In one aspect of the present disclosure, the weight ratio of the mixture of adenosine, D-panthenol and niacinamide is 1:0.3 to 5:0.2 to 8, preferably 1:0.5 to 4:0.3 to 6 (adenosine:D-panthenol:niacin amide). When the mixture of adenosine, D-panthenol, and niacinamide is combined at the specific weight ratio, the effect of preventing hair loss and promoting hair growth is excellent.

In one aspect of the present disclosure, the composition for preventing hair loss or promoting hair growth of the present disclosure may be used as a pharmaceutical composition, a quasi-drug composition, a cosmetic composition, or a health functional food composition. The composition of the present disclosure can be formulated in any form that can be generally applied to the skin, but is preferably formulated in the form of an external skin preparation. The composition of the present disclosure can be prepared in a formulation that can be applied to the skin, for example, in a liquid, cream, paste, or solid form.

A mixture of adenosine, D-panthenol, and niacinamide of the present disclosure; And any one or more selected from the group consisting of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid may be included in the composition or formulation as an anti-hair loss agent or hair growth promoter.

When the formulation of the present disclosure is in a liquid form, solvents, solubilizers, or emulsifying agents may be used as carrier components, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-Butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or sorbitan fatty acid ester may be used.

When the formulation of the present disclosure is a paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as the carrier ingredient. there is.

When the formulation of the present disclosure is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as the carrier component. In particular, in the case of a spray formulation, it may additionally contain a propellant such as chlorofluorohydrocarbon, propane/butane, or dimethyl ether.

In addition to the above active ingredients, the composition of the present disclosure may include ingredients commonly used as external agents that can be applied to the skin. For example, one or more additives selected from the group consisting of water, surfactants, moisturizers, lower alcohols, chelating agents, disinfectants, antioxidants, preservatives, colorants, and fragrances may be additionally included.

Additionally, the present disclosure provides a hair or scalp product containing the composition for preventing hair loss or promoting hair growth. The above hair or scalp products include hair growth agents, scalp clinic agents, scalp scaling agents, scalp massage agents, scalp care agents, detergents, shampoos, tonics, hair conditioners, hair lotions, gels, packs, creams, essences, powders, sprays, and oils. , may be any one selected from the group consisting of soap, ointment, hair styling agent, hair dye, perm agent, etc., but is not limited thereto.

In one aspect of the present disclosure, the composition for preventing hair loss or promoting hair growth may be prepared in the form of a hair tonic or hair lotion (Preparation Examples 1 to 3).

The composition for preventing hair loss or promoting hair growth of the present disclosure can preferably be used by a transdermal administration method such as directly applying or spraying to the skin.

In addition, the present disclosure provides a health functional food composition containing the composition for preventing hair loss or promoting hair growth. The health functional food composition can be prepared by methods commonly used in the art. In the above manufacturing process, it can be manufactured by adding raw materials and components commonly added in the industry. Additionally, the formulation of the health functional food can also be manufactured without limitation as long as it is a formulation recognized as a health functional food. The health functional food of the present disclosure can be manufactured in a variety of formulations, is made from food, has the advantage of having no side effects that may occur when taking drugs for a long time, and is highly portable, so it can prevent hair loss or promote hair growth. It can be taken as a supplement for:

Additionally, the present disclosure provides a mixture of adenosine, D-panthenol, and niacinamide; And hair loss prevention or hair growth comprising the step of administering to a subject a composition for preventing hair loss or promoting hair growth containing as an active ingredient at least one selected from the group consisting of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid. Provides methods of promotion. In one aspect of the present disclosure, the method for preventing hair loss or promoting hair growth may include treating the patient's scalp or hair with the composition for preventing hair loss or promoting hair growth.

As used in this disclosure, the term “administration” may mean introducing a composition of this disclosure by any suitable method. The administration route of the composition of the present disclosure can be administered through any general route as long as it can reach the target tissue. Preferably, it can be administered transdermally, and topical application is most preferable. The number of applications of the composition of the present disclosure may be determined according to prescription, need, or desire.

The amount of the composition for preventing hair loss or promoting hair growth of the present disclosure can be appropriately adjusted depending on individual differences such as age, degree of lesion, etc., and the formulation. Typically, an appropriate amount is applied to the scalp once or several times a day and used for one week to several months. It is desirable to do so. In the experimental example of the present disclosure, Composition 1 for hair loss treatment was used 5 times a week for 6 months, and as a result, it was confirmed that an excellent hair growth promotion effect was observed (Experimental Examples 4 and 5).

All ingredients described in the present disclosure are preferably subject to relevant laws and regulations of Korea, China, the United States, Europe, Japan, etc. (e.g., Regulations on Cosmetic Safety Standards, etc. (Korea), Cosmetic Safety Technical Regulations (China), etc. Do not exceed the maximum usage level specified in the Food Code (Korea), Food Additives Code (Korea), and Health Functional Food Code (Korea). That is, preferably, the pharmaceutical composition, quasi-drug composition, cosmetic composition, or health functional food composition according to the present disclosure contains the ingredients according to the present disclosure within the content limit permitted by the relevant laws and regulations of each country.

The composition for preventing hair loss and promoting hair growth of the present disclosure promotes the activity of dermal papilla cells, the mitochondrial activity of dermal papilla cells, and the activity of the Wnt/β-catenin signaling system through a combination of specific active ingredients, thereby exhibiting significant anti-hair loss and hair growth promoting effects. It creates a synergistic effect.

Hereinafter, to aid understanding of the present invention, it will be described in detail through examples. However, the embodiments according to the present invention may be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the present invention are provided to more completely explain the present invention to those with average knowledge in the field to which the present invention pertains.

Preparation Example 1: Composition 1 for hair loss treatment (hair tonic)

A hair tonic was prepared in a conventional manner according to the recipe shown in Table 1 below, by varying the weight ratio of the mixture of adenosine, D-panthenol, and niacinamide.

Ingredients ethanol castor oil glycerin Adenosine D-Panthenol Niacinamide Fragrances and Colors Purified water Weight ratio (%) Comparative Example 1 55 5 3 0 0 0 Appropriate amount remaining amount Comparative example 2 55 5 3 One One One Appropriate amount Example 1 55 5 3 1.6 0.9 0.5 Appropriate amount Example 2 55 5 3 0.8 1.5 0.7 Appropriate amount Example 3 55 5 3 0.75 0.75 1.5 Appropriate amount Example 4 55 5 3 0.6 0.8 1.6 Appropriate amount Example 5 55 5 3 0.3 One 1.7 Appropriate amount

Preparation Example 2: Composition 2 for hair loss treatment (hair tonic)

According to the prescription disclosed in Table 2 below, a hair tonic was prepared in a conventional manner by adding one each of Hewlett-Packard extract, Houttuynia cordata extract, zinc pyrithione, or salicylic acid.

Ingredients ethanol castor oil glycerin Adenosine D-Panthenol Niacinamide Hewlett-Packard Extract Houttuynia cordata extract zinc pyrithione salicylic acid Fragrances and Colors Purified water Weight ratio (%) Example 6 55 5 3 0.4 0.6 1.0 One Appropriate amount remaining amount Example 7 55 5 3 0.4 0.6 1.0 One Appropriate amount Example 8 55 5 3 0.4 0.6 1.0 One Appropriate amount Example 9 55 5 3 0.4 0.6 1.0 One Appropriate amount

Preparation Example 3: Composition 3 for hair loss treatment (hair lotion)

Hair lotion was prepared in a conventional manner according to the recipe shown in Table 3 below by adding one or more of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid to a mixture of adenosine, D-panthenol, and niacinamide.

Ingredients Weight ratio (%) Cetostearyl alcohol 2 Stearyltriethylammonium chloride 2 Hydroxyethyl Cellulose 0.5 Adenosine One D-Panthenol 1.5 Niacinamide 3 One or more of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid One Fragrances and Colors Appropriate amount Purified water Remaining amount (total 100)

Experimental Example 1: Effect of enhancing dermal papilla cell activity

Human-derived dermal papilla cells (DPCs) were purchased from Promocell. The DPCs were grown in DMEM (Hyclone Inc, UT, USA) containing 5% fetal bovine serum (FBS; Gibco, NY, USA), 100 units/mL of penicillin, and 100 μg/mL of streptomycin at 37°C and 5% CO _{2 .} Cultured under conditions. 3,000 cells/well of cultured DPCs were dispensed into a 96-well plate and cultured for 24 h under 0.1% serum conditions. Afterwards, the cultured cells were treated with DMSO (vehicle) diluted 1:1000 in serum-free DMEM as a control group, minoxidil 2 ㎍/㎖ as a positive control group, a mixture of adenosine, D-panthenol, and niacinamide, and a mixture of adenosine, D-panthenol, and niacinamide, as well as a mixture of Houttuynia cordata extract and Houttuynia cordata. One or more of the extract, zinc pyrithione, and salicylic acid as shown in Table 4 below. Treated and incubated for 1 day. After incubation, the degree of cell activity enhancement was evaluated using the CCK method. CCK-8 was added to the culture medium at a ratio of 1:10 and incubated for 1 hour. After 1 hour, the absorbance of each well was measured at 450 nm. All experiments were repeated three times and the average of the absorbance values was calculated. The results were expressed as a percentage of the treatment group compared to the control group as 100.

Treatment group Dermal papilla cell activity (%) Untreated group 100 Adenosine (0.06%) + D-Panthenol (0.08%) + Niacinamide (0.16%) 149.7 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Sewage extract (0.1%) 168.6 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Houttuynia cordata extract (0.1%) 169.4 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Zinc pyrithione (0.1%) 160.3 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Salicylic acid (0.1%) 166.3 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Sewage extract (0.05%) + Zinc pyrithione (0.05%) 177.4 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Sewage extract (0.05%) + Salicylic acid (0.05%) 178.0 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Houttuynia cordata extract (0.05%) + Zinc pyrithione (0.05%) 183.7 Adenosine (0.04%) + D-Panthenol (0.06%) + Niacinamide (0.1%) + Houttuynia cordata extract (0.05%) + Salicylic acid (0.05%) 187.7

Looking at the results disclosed in Table 4 above, when the mixture of adenosine, D-panthenol, and niacinamide is further treated with one or more of Hewlett-Packard extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid, the dermal papilla cell activation ability It was confirmed that a significant synergistic effect occurred in enhancement.

Experimental Example 2: Effect of promoting mitochondrial activity in hair papilla cells

Human-derived dermal papilla cells (DPCs) were purchased from Promocell. The DPCs were grown in DMEM (Hyclone Inc, UT, USA) containing 5% fetal bovine serum (FBS; Gibco, NY, USA), 100 units/mL of penicillin, and 100 μg/mL of streptomycin at 37°C and 5% CO _2. Cultured under conditions. 3,000 cells/well of cultured DPCs were dispensed into a 96-well plate and cultured for 24 h under 0.1% serum conditions. Afterwards, the cultured cells were treated with DMSO (vehicle) diluted 1:1000 in serum-free DMEM as a control group, minoxidil 2 ㎍/㎖ as a positive control group, a mixture of adenosine, D-panthenol, and niacinamide, and a mixture of adenosine, D-panthenol, and niacinamide, as well as extract from Houttuynia cordata. One or more of the extract, zinc pyrithione, and salicylic acid as shown in Table 5 below. Treated and incubated for 1 day. After incubation, the degree of cell proliferation was evaluated using the JC-1 method. JC-1 was added to the culture medium at 1:100 and incubated for 1 hour. After 1 hour, the fluorescence of each well was measured at 590nm and 530nm. All experiments were repeated three times and the average of fluorescence values was calculated. The results were expressed as a percentage of the treatment group compared to the control group as 100.

Treatment group Dermal papilla cell mitochondrial activity (%) Untreated group 100 Adenosine (0.06%) + D-Panthenol (0.08%) + Niacinamide (0.16%) 160.5 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Sewage extract (0.1%) 169.4 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Houttuynia cordata extract (0.1%) 177.5 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Zinc pyrithione (0.1%) 173.6 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Salicylic acid (0.1%) 179.3 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Sewage extract (0.05%) + Zinc pyrithione (0.05%) 188.3 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Sewage extract (0.05%) + Salicylic acid (0.05%) 183.7 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Houttuynia cordata extract (0.05%) + Zinc pyrithione (0.05%) 186.2 Adenosine (0.04%) + D-Panthenol (0.06%) + Niacinamide (0.1%) + Houttuynia cordata extract (0.05%) + Salicylic acid (0.05%) 197.3

Looking at the results disclosed in Table 5 above, when the mixture of adenosine, D-panthenol, and niacinamide is further treated with one or more of Hewlett-Packard extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid, the mitochondrial activity of hair papilla cells It was confirmed that a significant synergistic effect occurred in enhancement.

Experimental Example 3: Effect of regulating dermal papilla cell activation signal (Wnt/β-catenin)

In general, the Wnt/β-catenin signal activated in the hair papilla occurs when the hair goes from the catagen phase to the active phase during the hair growth cycle, that is, from the beginning of hair growth to the active phase when hair growth occurs. During the telogen and catagen phases, Wnt/β-catenin signaling weakens or disappears, causing hair follicles to degenerate and hair loss to occur. Therefore, through this experiment, how much the group treated with a mixture of adenosine, D-panthenol, and niacinamide, and one or more of Houttuynia cordata extract, zinc pyrithione, and salicylic acid in the mixture of the present disclosure contributes to the amplification of the Wnt/β-catenin signal Confirmed.

Wnt3a protein was used as a positive control to amplify the Wnt/β-catenin signal, and DMSO was used as a negative control. In a 96-well culture plate, ³ One or more types were treated as shown in Table 6 below. Next, a reporter assay was performed using Promega's Luciferase assay kit (E1960). The experiment was performed according to the manufacturer's test method, and Wnt/β-catenin promoter activity was measured using a luminometer Victor (PerkinElmer, Waltham, Massachusetts, USA).

Treatment group Ability to activate Wnt/β-catenin signaling for hair follicle activation (%) Untreated group 100 Recombinant wnt3a 100ng/ml 248.9 Adenosine (0.06%) + D-Panthenol (0.08%) + Niacinamide (0.16%) 1363.4 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Sewage extract (0.1%) 1590.1 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Houttuynia cordata extract (0.1%) 1530.4 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Zinc pyrithione (0.1%) 1571.0 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Salicylic acid (0.1%) 1573.1 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Sewage extract (0.05%) + Zinc pyrithione (0.05%) 1825.9 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Sewage extract (0.05%) + Salicylic acid (0.05%) 1697.7 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Houttuynia cordata extract (0.05%) + Zinc pyrithione (0.05%) 1794.8 Adenosine (0.04%) + D-Panthenol (0.06%) + Niacinamide (0.1%) + Houttuynia cordata extract (0.05%) + Salicylic acid (0.05%) 1889.7

Looking at the results disclosed in Table 6 above, when the mixture of adenosine, D-panthenol, and niacinamide is further treated with one or more of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid, Wnt/β- It was confirmed that it synergistically enhances the activity of the catenin signaling system. Since the Wnt/β-catenin signal is a hair growth promoting signal of hair follicle stem cells, it can be seen from the above experimental results that the composition of the present disclosure exhibits a significant synergistic effect in promoting hair growth.

Experimental Example 4: Hair growth effect of composition 1 (hair tonic) for treating hair loss or promoting hair growth

The hair growth effect of Composition 1 (hair tonic) for treating hair loss or promoting hair growth of the present invention was tested on a total of 70 men and women with weak hair who had significantly less hair than normal or had hair loss symptoms. A total of 70 men and women were divided into 7 groups of 10 each and used Comparative Examples 1 and 2 and Examples 1 to 5, and were used on the hair and scalp 5 times a week for 6 months. After use, researcher evaluation was conducted through clinical photography and phototricogram. The researcher's evaluation through clinical photographs was based on three items: 'good', 'slight', or 'no change' for comparative examples and examples 2 and 6 months after application (Good: 50% to 75% improvement, Slight: 25%~50% improvement, No change: No change). Evaluation of the number of hairs per unit area and average hair thickness through phototricogram was measured for comparative examples and examples 6 months after application, and the results are shown in Table 7.

sample Researcher evaluation through clinical photographs phototricogram No change Slight Good Number of hairs per unit area (piece/cm ² ) Average hair thickness (μm) (number of people) (number of people) (number of people) february June february June february June 0 month June 0 month June Comparative Example 1 8 7 2 3 0 0 125.2±20 126.9±14 43.6±8 45.2±3 Comparative Example 2 7 4 2 4 One 2 125±16 134±10 45.3±6 55.9±4 Example 1 6 4 3 3 One 3 119±10 135±10 44.2±10 53.4±3 Example 2 7 3 2 4 One 3 128.2±17 137±23 44.7±8 54.3±4 Example 3 3 2 5 4 2 4 124.4±15 153±17 41.3±7 62.5±2 Example 4 2 2 4 3 4 5 123.1±13 179±19 44±8 77.2±8 Example 5 2 One 4 4 4 5 120±20 169±17 42.5±4 69±6

Looking at the results disclosed in Table 7, it was confirmed that when treated with a mixture of adenosine, D-panthenol, and niacinamide, the number of hairs per unit area and the average hair thickness were improved. In particular, it was confirmed that when adenosine, D-panthenol, and niacinamide were mixed and treated at a specific weight ratio according to the present disclosure, the effect of improving the number of hairs per unit area and the average hair thickness was significantly improved.

Experimental Example 5: Hair growth effect of composition 2 (hair tonic) for treating hair loss or promoting hair growth

The hair growth effect of composition 2 (hair tonic) for hair loss treatment or hair growth promotion of the present invention was tested on a total of 60 men and women with weak hair who had significantly less hair than normal or had hair loss symptoms. A total of 60 men and women were divided into 6 groups of 10 each and used Comparative Example 1 and Examples 6 to 9, and were used on the hair and scalp 5 times a week for 6 months. After use, researcher evaluation was conducted through clinical photography and phototricogram. The researcher's evaluation through clinical photographs was based on three items: 'good', 'slight', or 'no change' for comparative examples and examples 2 and 6 months after application (Good: 50% to 75% improvement, Slight: 25%~50% improvement, No change: No change). Evaluation of the number of hairs per unit area and average hair thickness through phototricogram was measured for comparative examples and examples 6 months after application, and the results are shown in Table 8.

sample Researcher evaluation through clinical photographs phototricogram No change Slight Good Number of hairs per unit area (piece/cm2) Average hair thickness (μm) (number of people) (number of people) (number of people) february June february June february June 0 month June 0 month June Comparative Example 1 9 8 One 2 0 0 133.2±10 130.8±10 40.6±4 40.4±6 Example 6 One One 3 4 6 5 131.8±10 199±13 44.5±3 86.9±3 Example 7 One 0 One 2 8 8 129.8±19 188±18 42.8±5 88.3±7 Example 8 2 0 4 3 4 7 134.5±10 185±19 45.4±8 83.5±9 Example 9 0 0 3 2 7 8 132.3±18 190±17 43.3±2 86±2

Considering the results disclosed in Table 8, as in Examples 6 to 9, the mixture of adenosine, D-panthenol, and niacinamide was treated by additionally including one or more of Hewlett-Packard extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid. In this case, it was confirmed that the number of hairs per unit area and the average hair thickness were significantly increased compared to Examples 1 to 5, showing a significant synergy effect in preventing hair loss and promoting hair growth.

Formulation examples such as hair tonic or hair lotion are only examples of compositions for preventing hair loss or promoting hair growth of the present disclosure, and the scope of the composition of the present disclosure is not limited to these formulations, as is known in the art to which the present invention pertains. It will be self-evident to those with knowledge.

Claims (12)

A mixture of adenosine, D-panthenol and niacinamide; and
Contains as an active ingredient at least one selected from the group consisting of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid,
A composition for preventing hair loss or promoting hair growth wherein the weight ratio of the mixture is 1:0.3~5:0.2~8 (adenosine:D-panthenol:niacinamide). The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the content of the active ingredient is 0.0001 to 10% by weight based on the total weight of the composition. The composition of claim 1, wherein the composition is a composition for preventing hair loss or promoting hair growth that enhances the activity of dermal papilla cells. According to claim 1, The composition is a composition for preventing hair loss or promoting hair growth that promotes mitochondrial activity of dermal papilla cells. According to claim 1, The composition is a composition for preventing hair loss or promoting hair growth that promotes the activity of the Wnt/β-catenin signaling system. The composition of claim 1, wherein the composition is a pharmaceutical composition for preventing hair loss or promoting hair growth. The composition of claim 1, wherein the composition is a quasi-drug composition for preventing hair loss or promoting hair growth. The composition according to claim 1, wherein the composition is a cosmetic composition. A hair or scalp product comprising the composition for preventing hair loss or promoting hair growth according to claim 1. The method of claim 9, wherein the products include hair growth agents, scalp clinic agents, scalp scaling agents, scalp massage agents, scalp care agents, detergents, shampoos, tonics, hair conditioners, hair lotions, gels, packs, creams, essences, powders, and sprays. , hair or scalp products selected from the group consisting of oils, soaps, ointments, hair styling agents, hair dyes, and perm agents. A mixture of adenosine, D-panthenol and niacinamide; and
Contains as an active ingredient at least one selected from the group consisting of Houttuynia cordata extract, Houttuynia cordata extract, zinc pyrithione, and salicylic acid,
A composition for hair where the weight ratio of the mixture is 1:0.3~5:0.2~8 (adenosine:D-panthenol:niacinamide). The composition for hair according to claim 11, wherein the composition is for strengthening hair roots.