KR101724526B1 - Cosmetic Composition Containing Fermentative Extract of Chenopodium quinoa Willd as Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a fermented extract of Chenopodium quinoa Willd . As an active ingredient. According to the present invention, a fermented extract of Chenopodium quinoa Willd . Can be used for whitening, skin aging, It is possible to provide a cosmetic composition having an excellent effect on the skin.

Description

[0001] The present invention relates to a cosmetic composition containing an extract of Fermented Quinona as an active ingredient,

The present invention relates to a cosmetic composition for improving whitening, skin aging, and skin wrinkles containing a fermented extract of Chenopodium quinoa Willd . As an active ingredient.

A variety of physical and chemical changes occur in human skin during the aging process. The cause of this phenomenon is divided into intrinsic aging and photo-aging. Free radicals can be caused by activation of ultraviolet rays, stress, disease state, environmental factors, wounds, and aging. When such state is deepened, the antioxidant defense network existing in the living body is destroyed, And aging. More specifically, lipids, proteins, polysaccharides and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging.

Skin color of a person is determined by melanin, hemoglobin, carotene, etc. Among them, melanin plays the most important role. In addition to determining human skin color, melanin also performs skin protection functions such as ultraviolet absorbing action, free radical scavenger and the like. However, excessive production of melanin due to external environmental changes such as overexposure of ultraviolet rays, air pollution, stress, and the like causes pigmentation in the skin, which causes blackening of skin, freckles, and the like. Blackening of the skin is caused by the reaction of the skin cells to internal and external factors, and a typical factor is due to ultraviolet exposure. That is, when the skin is exposed to ultraviolet rays, tyrosinase is activated. By the oxidation process in which tyrosinase acts on tyrosine present in skin tissue to generate dopa (DOPA) and dopaquinone, In melanocytes in melanocytes, a polymer called melanin is synthesized. This melanin is transferred to keratinocytes, which are keratinocytes of skin, and reaches skin surface by keratinization process to protect skin from ultraviolet rays. Thus, melanin is an essential UV inhibitor in the human body, and it also plays an effective role as an effective radical scavenger to remove various radicals that modify biological components such as proteins, lipids, and nucleic acids.

In particular, the oxidation of proteins can cause collagen (collagen), hyaluronic acid, elastin, proteoglycan, fibronectin, and the like that are severely hyperinflammatory reactions and skin elasticity If this gets worse, DNA mutations can lead to mutations, cancer, and immune deficiency. Therefore, it is necessary to protect the cell membrane by destroying the free radicals mediated by free radicals, ultraviolet rays, and inflammation that occur during the metabolism of the body, and to regenerate already damaged cells by vigorous metabolism The skin can quickly recover and maintain healthy skin.

Aging involves free radicals as well as enzyme called matrix metalloproteinase (MMP), a collagenase. That is, synthesis and degradation of extracellular matrix such as collagen in vivo are appropriately controlled, but collagen synthesis is reduced as aging progresses, and expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, And the wrinkles are formed. These degrading enzymes are also activated by ultraviolet irradiation. Therefore, there is a demand for development of a substance capable of modulating MMP expression induced in the cell or inhibiting its activity. Oxygen is an indispensable substance in our life with water, but once it enters our body, it turns into highly active oxygen by various causes to oxidize blood vessel cells. Today, about 90% of the causes of the disease are attributed to this active oxygen. The human body is composed of about 60 trillion cells, and the cell membrane is formed as an impetium, so active oxygen is easily connected. When the oxidation reaction occurs, the unsaturated fatty acid changes to lipid peroxide. This lipid peroxidase damages gene DNA, RNA, protein, cell membrane, and cell structure, leading to cancer, causing many adult diseases, and ultimately causing aging. It is called antioxidant substances that act to prevent cells from oxidizing by neutralizing or eliminating these free radicals. Generally, when peroxidation is caused by lipid active oxygen, which is a constituent of skin cells, the cell membrane structure is modified, resulting in cell permeability, fluidity, inactivation of membrane enzymes and transport proteins, cleavage of protein chains, DNA and RNA damage Skin cells are known to cause aging. For this reason, efforts to remove active oxygen have continued, and as a result, many sulfated materials have been developed. Materials known to have antioxidative action so far include ascorbic acid, beta-carotene, dibutylhydroxyltoluene (BHT), and DL-alpha-tocopherol . However, since these materials are used as synthetic materials, they cause problems of skin safety when they are used for a long period of time or when they are used in large amounts, or they are limited in their use due to problems in stability when they are contained in cosmetics. to be. Many researchers are working hard to solve these problems.

Korean Patent Laid-Open Publication No. 2009-0092841 describes various skin scientific uses of Quinoa algae extract.

The present inventors have searched various skin physiological properties of natural plant ingredients having both aging and whitening effects. As a result, it has been found that the extract of Chenopodium quinoa Willd . In natural plant extracts has DPPH radical scavenging effect, tyrosinase ) Inhibitory activity, elastase inhibitory activity, promoting collagen synthesis, and inhibiting MMP-1 production, thereby effectively improving whitening, skin aging, and skin wrinkles.

Above object is achieved by a cosmetic composition comprising a fermentation extract of Quinoa (Chenopodium quinoa Willd.).

Preferably, the above-mentioned quinoa fermentation extract is fermented for 5 to 10 days while maintaining a pH of 5 to 8 under a temperature condition of 28 to 42 DEG C as a bacterium selected from the group consisting of yeast, lactic acid bacteria, Bacillus sp. .

Preferably, the fermented extract of Chenopodium quinoa Willd . Is contained in an amount of 0.0001 to 30.0% (w / w) based on the total weight of the cosmetic composition.

Preferably, the fermented extract of Chenopodium quinoa Willd . Has a DPPH radical scavenging activity, a tyrosinase inhibitory activity, an elastase inhibitory activity, a collagen synthesis promotion effect, an MMP-1 production inhibitory activity Effect.

Preferably, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray ≪ / RTI >

The extract of Chenopodium quinoa Willd. , Which is an active ingredient contained in the cosmetic composition of the present invention, has a DPPH radical scavenging effect, a tyrosinase inhibitory activity, an elastase inhibitory activity, a collagen synthesis promoting effect, The effect of inhibiting MMP-1 production is excellent, so that the effect of whitening, skin aging, and skin wrinkles is excellent.

The present invention relates to a cosmetic composition comprising a fermented extract of Chenopodium quinoa Willd . As an active ingredient, wherein the Chenopodium quinoa Willd. Used in the present invention belongs to the genus Chenopodium and belongs to Chenopodium, In addition to the existing and cultivated Chenopodium quinoa, most of them are weed species distributed all over the world. The cultivar, quinoa, is allotetraploid while the other species (Chenopodiumspecies) are diploid, tetraploid, hexaploid or octoploid. Quinoa was cultivated for at least 5000 years in the Andes region of South America for a long time before the Inca Empire, and the major crops in the region before the Colombian period were corn, potatoes and quinoa. The C3 crop, Quinoa, is said to grow well under dry conditions in high altitudes above 3000 m above sea level where other crops do not grow well. Quinoa is a little smaller than rice and is easily cooked and is rich in protein and starch vitamins and minerals. Quinoa, known as the 'Super Grain' of the Inca Empire, has been a major agricultural product in the Andean region for thousands of years, such as Ecuador, Peru and Bolivia, but has only survived in recent times with only a handful of self-sufficiency in some farm households. Since then, nutritional value has been renewed and is being sold in the international grain market at a rapid pace since 1980, thanks to the efforts of breed improvement and dissemination efforts of world-class food companies and South American civilian organizations.

The fermented extract of Chenopodium quinoa Willd . According to the present invention can be prepared as follows. After washing and drying the wheat quinoa, the following microorganism culture medium is added in an amount of 1 to 50 g / L, and a microorganism selected from the group consisting of yeast, lactic acid bacteria, Bacillus subtilis and mixtures thereof is added in an amount of 10,000 to 100,000 CFU / L Lt; / RTI > The incubation temperature may be in the range of 28 to 42 DEG C, and preferably 30 to 37 DEG C. The pH is 5 to 8 and aerobically or usually anaerobic conditions are incubated for about 5 to 10 days. Which can be obtained through aging and filtration.

As the fermentation strains used in the fermentation process, one or more selected from the group consisting of Lactobacillus sp., Aspergillus sp., And Bacillus sp. Can be used.

Examples of the Lactobacillus strain include Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus bulgaricus, Lactobacillus casei ), Lactobacillus casei subsp. Alactosus, Lactobacillus caseisubsp. Casei, Lactobacillus casei subsp. Pseudoplantarum (subspecies), Lactobacillus casei subsp. Lactobacillus casei subsp. Rhamnosus, Lactobacillus casei subsp; tolerans, Lactobacillus catenaformis, Lactobacillus cellobiosus, Lactobacillus casei subsp. Lactobacillus collinoides, Lactobacillus < RTI ID = 0.0 > Lactobacillus coryniformis subsp. Coryniformis, Lactobacillus corneumiformis (subspecies), Lactobacillus coryniformis, Lactobacillus coryniformis, Lactobacillus coryniformis (subspecies) Lactobacillus licheniformis, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus delbrueckii, Lactobacillus spp., Lactobacillus spp., Lactobacillus spp., Lactobacillus spp., Lactobacillus spp. , Lactobacillus fermentum, Lactobacillus fructivorans, Lactobacillus fructosus, Lactobacillus helveticus, Lactobacillus heterohiochii, Lactobacillus spp., Lactobacillus spp. Lactobacillus hilgardii, Lactobacillus < RTI ID = 0.0 > Lactobacillus homohiochii, Lactobacillus jensenii, Lactobacillus lactis, Lactobacillus leichmannii, Lactobacillus mali, Lactobacillus maltus, Lactobacillus spp., Lactobacillus spp., Lactobacillus spp. maltolomicus, Lactobacillus minutus, Lactobacillus plantarum, Lactobacillus rogosae, Lactobacillus ruminis, Lactobacillus sake, Lactobacillus spp., Lactobacillus spp., Lactobacillus spp. Lactobacillus salivarius, Lactobacillus salivarius (subspecies), Salicinia (Lactobacillus salivariussubsp. salicinius, Lactobacillus salivarius subsp. salivarius, Lactobacillus trichodes, Lactobacillus viridescens, Lactobacillus vitulinus, Lactobacillus spp., Lactobacillus spp. And Lactobacillus xylosus. Among them, Lactobacillus bulgaricus is more preferable.

As an example of the Aspergillus strain, any one or more selected from Aspergillus oryzae, Aspergillus niger and Aspergillus usami can be used, and more preferably, Aspergillus oryzae, Aspergillus niger, Aspergillus usami, Aspergillus oryzae is preferred.

Examples of the above Bacillus strains include Bacillus acidocaldarius, Bacillus alcalophilus, Bacillus alvei, Bacillus anthracis, Bacillus badius, Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus fastidiosus, Bacillus firmus, Bacillus spp. , Bacillus globisporus subsp. Marinus, Bacillus globisporus subsp. Globisporus, Bacillus globisporus subsp. Globisporus, Bacillus globisporus subsp. Marinus, Bacillus globisporus (subspecies) Bacillus insolitus, Bacillus larvae, Bacillus laterosporus, Bacillus lentimorbus, Bacillus lentus, Bacillus licheniformis, Bacillus macerans, Bacillus macquariensis, Bacillus megaterium (Bacillus spp.), Bacillus spp. Bacillus megaterium, Bacillus mycoides, Bacillus pantothenticus, Bacillus pasteurii, Bacillus polymyxa, Bacillus popilliae, Bacillus pumilus, One or more selected from Bacillus pumilus, Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, and Bacillus thuringiensis may be used. Preferably, Bacillus subtilis is preferred.

According to the present invention, the fermented extract of Chenopodium quinoa Willd . May be contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition, more preferably the fermented extract of Chenopodium quinoa Willd . Is contained in an amount of 0.001 to 10% by weight based on the total weight of the cosmetic composition. When the content of the fermented quinoa extract is less than 0.0001% by weight, the effect of improving the skin is not exhibited. When the content of the fermented extract is more than 30.0% by weight, the degree of improvement of the skin against the increase of the content is insignificant. And it is not economical.

The components contained in the cosmetic composition of the present invention may contain components commonly used in cosmetic compositions in addition to the above-mentioned effective components, for example, conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and perfumes, . The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powdered foundation, emulsion foundation, wax foundation, pack, massage cream and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component . When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters. In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used. When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether. When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, washed or rinsed with water. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, and the surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel and a cleansing pack, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

On the other hand, the cosmetic process of the present invention refers to all cosmetic processes using the cosmetic composition of the present invention. That is, all methods known in the art using a cosmetic composition belong to the cosmetic method of the present invention. The cosmetic composition of the present invention may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention. The cosmetic composition according to the present invention may be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

The cosmetic extract of the present invention has excellent wrinkle-improving effect, whitening effect and anti-aging effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for describing the present invention in more detail, and the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention.

Hereinafter, the present invention will be described in detail with reference to the following Examples and Experimental Examples. However, the present invention is described in detail with reference to the following Examples and Experimental Examples, and the scope of the present invention is not limited thereto.

Manufacturing example  One. Quinoa ( Chenopodium  quinoa Willd . ) Preparation of fermented extract

After washing, the shrunken quinoa algae (500 g) was refluxed with 70% (v / v) aqueous ethanol solution for 5 hours three times, cooled, and filtered through Whatman # 4 filter paper. The filtrate extract (2.5 L 70% EtOH) was concentrated under reduced pressure at 50 캜 or lower to adjust the moisture content to 50% by adding water to obtain a fermentation raw material so that the fermentation microorganism can be cultured well. Subsequently, the obtained fermentation raw material was sterilized at 121 DEG C for 15 minutes.

Bacillus subtilis was inoculated in 3% by weight of LB medium (Difco, USA) and cultured at 37 ° C. in aerobic condition at 37 ° C. for 10 days. Then, the culture solution was filtered using 0.25 μM filter paper, and the filtrate was aged at 4 ° C. for 10 days at a low temperature, followed by final filtration using a 0.25 μM filter paper. The thus-prepared fermentation broth is referred to as "quinoa fermentation extract ", and the quinoa fermentation extract used in the following Experimental Examples was maintained at a final concentration of 1 g / 100 ml.

The above preparation examples are for the purpose of explaining the invention in more detail, and the scope of the strains used for the production of the fermented quinoa extract is not limited thereto.

Comparative Manufacturing Example  One. Quinoa ( Chenopodium  quinoa Willd . ) Preparation of extract

After washing, the shrunken quinoa algae (500 g) was refluxed with 70% (v / v) aqueous ethanol solution for 5 hours three times, cooled, and filtered through Whatman # 4 filter paper. The filtered extract was concentrated under reduced pressure at 50 ° C or less to maintain a final concentration of 1 g / 100 ml.

Experimental Example  1. Tyrosinase ( tyrosinase ) Inhibition activity measurement

In order to measure tyrosinase inhibitory activity of Preparation Example 1 and Comparative Preparation Example 1, tyrosinase inhibitory activity was measured under laboratory conditions. As a substrate, 40 μl of 1.5 mmol / l tyrosine (Sigma, USA) dissolved in sodium phosphate buffer (pH 6.8) was used. Production Example 1 and Comparative Production Example 1 were each diluted with a 0.05 M sodium phosphate buffer solution (pH 6.8) to prepare a sample solution for each concentration (0.5%, 1.0%). To the solution was added 20 μl of tyrosinase (Sigma, 1500 U / ml), and the mixture was reacted at 37 ° C. for 15 minutes. Then, the mixture was reacted at 490 nm Absorbance was measured. The activity inhibition rate of tyrosinase was calculated according to the following formula (1).

Name of sample Treatment concentration (ppm) Inhibition rate of tyrosinase activity (%) Comparative Preparation Example 1 500 16.195 Production Example 1 45.881

As shown in Table 1, the tyrosinase activity inhibition rate of Preparation Example 1 according to the present invention is excellent.

Experimental Example  2. DPPH Radical  Measurement of scavenging activity

In order to measure the antioxidative effects of the above Preparation Example 1 and Comparative Preparation Example 1, the antioxidative activity was measured by the DPPH method under laboratory conditions.

The DPPH method measures the antioxidative activity by reducing power using a free radical called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The degree of free radical scavenging at a wavelength of 560 nm was measured by comparing the degree of decrease in absorbance with the absorbance of the blank test solution by DPPH reduction by the test substance. The reagent used was 2,2-Di (4-tert-octylphenyl) -1- and dissolved in methanol as a 0.2 mM solution of picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) to make 100 ml.

As a measurement method, 0.15 ml of a 0.2 mM DPPH solution and 0.15 ml of a sample solution are added to a 96-well plate, stirred rapidly, and incubated at 25 DEG C for 10 minutes. Thereafter, the absorbance St at 560 nm is measured. The blank test is carried out in the same manner as above using distilled water instead of the sample solution, and the absorbance Bt is measured. The blank of the sample solution is measured by the same procedure using methanol instead of 0.2 mM DPPH solution to determine the absorbance Bo.

The results were calculated according to Equation (2), and the results are shown in Table 2.

St: absorbance at 560 nm after free radical scavenging of the sample solution

Bt: absorbance at 560 nm after free radical scavenging of the blank test solution

So: the absorbance at 560 nm before the reaction in the absence of free radicals in the sample solution

Bo: absorbance at 560 nm before the reaction in the absence of the free radical of the blank test solution

Name of sample Treatment concentration (ppm) Scavenging ability (%) Comparative Preparation Example 1 1000 61.251 Production Example 1 80.931 Comparative Preparation Example 1 500 40.812 Production Example 1 63.715 Comparative Preparation Example 1 100 9.453 Production Example 1 49.643

As shown in Table 2, it can be seen that the free radical scavenging ratio of Preparation Example 1 according to the present invention is excellent.

Experimental Example  3. Elastase ( Elastase ) Inhibition activity measurement

Elastase is an enzyme that degrades elastin, an important substrate in maintaining skin elasticity in the dermis. Elastase is also a nonspecific hydrolytic enzyme capable of degrading collagen, another important substrate protein. Thus, the elastase inhibitor has a function of improving the wrinkles of the skin, and the ursolic acid and the like are used as the elastase inhibitor. Elastase is known to be the main cause of wrinkles by decomposing elastin, the insoluble elastic fiber protein of animal connective tissue, and breaking the network structure of dermal tissue of skin. In the dermis of the skin, elastin related to the elasticity of collagen and skin forms a network structure. As the network structure is broken, the elastin is decomposed by elastase, and the skin is wrinkled and wrinkles are generated. do. Therefore, skin aging can be inhibited by decreasing the activity of elastase, an elastin degrading enzyme which is one of the major causes of skin aging. N-succinyl- (L-Ala) 3-p-nitroanilide, S4760, Sigma) The amount of p-nitroanilide produced from the substrate at 37 ° C for 20 minutes was measured at 445 nm. 500 μL of a solution of porcine pancreas elastase (E1250, Sigma) 2.5 U / mL) dissolved in 2 mM tris-HCl buffer (pH 8.6) was added to the test tube, and 50 mM N-succinyl- (L-Ala) 3-p-nitroanilide (500 μg / mL) dissolved in tris-HCl buffer (pH 8.6) was added and reacted for 20 minutes. Elastase inhibitory activity was calculated by the following equation (3) as the absorbance reduction ratio of the sample solution and the non-added sample, and the results are shown in Table 3 below.

Name of sample Treatment concentration (ppm) Elastase inhibitory activity (%) Comparative Preparation Example 1 100 5.129 Production Example 1 20.229 Comparative Preparation Example 1 500 17.815 Production Example 1 46.713 Comparative Preparation Example 1 1000 33.782 Production Example 1 68.192

Experimental Example  4. Results of measurement of collagen biosynthesis using ELISA kit

Human Fibroblast was cultured in DMEM (Dulbecco ' s Modified) containing 10% FBS, Penicillin (50 U / ml), Streptomycin (50 / ml) in a cell incubator maintained at a constant humidity in a 5% Eagle's Medium, Gibco, USA), and the cells were seeded at a density of 1 × 10 ⁵ cells / ml in a 24-well plate (500 μl) for 24 hours. Quantification of collagen type I in fibroblasts was performed by immunosorbent assay (ELISA) using Procollagen Type I c-peptide EIA kit (TaKaRa, japan) for 48 hours The cell culture supernatant as above cultured was carefully collected. First, 100 μl of the culture supernatant, which was cultured on a strip coated with antibody against Procollagen Type I cpeptide for 48 hours, was added and reacted at 37 ° C for 2 hours. After the reaction, unreacted material was removed by washing three times using a washing buffer, and 100 μl of blocking buffer was added thereto, followed by reaction at 37 ° C for 2 hours. After reaction, unreacted material was removed by washing three times using a washing buffer, and a solution in which POD was conjugated to another antibody binding to 'Procollagen Type I c-peptide' (Antiobody- POD conjugate solution) was added and reacted at 37 ° C for 2 hours. After the reaction, the unreacted material was removed by washing three times using a washing buffer, the same amount of substrate solution was added, and the reaction was carried out for 30 minutes. Then, stop solution (1N H2SO4) Terminated. The absorbance of the strip was measured at 450 nm, and the amount of newly synthesized collagen was measured by PICP in comparison with the collagen standard. The results are shown in Table 4.

Name of sample Treatment concentration (ppm) Collagen synthesis rate (%) Comparative Preparation Example 1 100 15.151 Production Example 1 22.846 Comparative Preparation Example 1 500 21.921 Production Example 1 40.593 Comparative Preparation Example 1 1000 29.935 Production Example 1 71.241

Experimental Example  5. MMP -1 production inhibitory effect

It was determined whether or not the above Production Example 1 and Comparative Production Example 1 had the effect of inhibiting the production of collagenase, MM P-1. Human normal skin cells, fibroblasts (Korean Cell Line Bank, Korea), were inoculated into 48-well microplates (Nunc, Denmark) at a density of 1 × 10 ⁶ cells per well and cultured in DMEM medium (Sigma, After culturing for 24 hours, the above Preparation Example 1 and Comparative Preparation Example 1 were further cultured for 48 hours in a serum-free DMEM medium supplemented with a final concentration of 100 占 퐂 / ml. After the incubation, the supernatant of each well was collected and the amount (ng / ml) of the newly synthesized MM P-1 was measured using MM P-1 assay kit (Amersham, 1 production inhibition rate (%) was calculated, and the results are shown in Table 5.

Name of sample Treatment concentration (ppm) Inhibition rate of MMP-1 production (%) Comparative Preparation Example 1 100 10.188 Production Example 1 25.321 Comparative Preparation Example 1 500 16.126 Production Example 1 47.125 Comparative Preparation Example 1 1000 24.357 Production Example 1 61.926

Prescription example  1. Cream

The prescription examples of creams in the cosmetic preparations containing Preparation Example 1 and Comparative Preparation Example 1 are shown in Table 6 below.

ingredient content
(Unit: wt%) Comparative Example  One Comparative Example  2 Formulation Example  One -
Glycerin monostearate
Cetearyl alcohol
Stearic acid
Wax
Polysorbate 60
Sorbitan stearate
Hardened vegetable oil
Squalane
Mineral oil
Trioctanoin
Dimethicone
Sodium magnesium silicate
glycerin
Betaine
Triethanolamine
Sodium hyaruronate
Preservative, fragrance, pigment
Distilled water Comparative Preparation Example 1
Glycerin monostearate
Cetearyl alcohol
Stearic acid
Wax
Polysorbate 60
Sorbitan stearate
Hardened vegetable oil
Squalane
Mineral oil
Trioctanoin
Dimethicone
Sodium magnesium silicate
glycerin
Betaine
Triethanolamine
Sodium hyaruronate
Preservative, fragrance, pigment
Distilled water Production Example 1
Glycerin monostearate
Cetearyl alcohol
Stearic acid
Wax
Polysorbate 60
Sorbitan stearate
Hardened vegetable oil
Squalane
Mineral oil
Trioctanoin
Dimethicone
Sodium magnesium silicate
glycerin
Betaine
Triethanolamine
Sodium hyaruronate
Preservative, fragrance, pigment
Distilled water 2.0
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
Balance

Experimental Example  1. Wrinkle improvement effect evaluation

The wrinkle-improving effect was evaluated by an actual use test using Comparative Examples 1 and 2 and the cream of Formulation Example 1. Thirty women aged 30-40 years were randomly divided into two groups. The creams of Comparative Examples 1 and 2 and Formulation 1 were washed twice a day, morning and evening, and a proper amount of cream was applied continuously for 2 months around the eyes. The wrinkle improvement effect of each subject was evaluated by visual observation. The experimental results are shown in Table 7 below.

Wrinkle improvement effect
Great Wrinkle improvement effect
slightly Wrinkle improvement effect
none Comparative Example 1 3 5 22 Comparative Example 2 3 8 17 Formulation Example 1 15 8 7

Example  2. Evaluation of whitening effect

The whitening effect of the cosmetic composition of the present invention was evaluated using actual use tests by using the creams of Comparative Examples 1 and 2 and Formulation Example 1. Twenty women aged 30 to 40 years were panel tested. The panels of Comparative Examples 1 and 2 and the cream of Formulation 1 were washed twice daily in the morning and evening, and then an appropriate amount of the cream was continuously applied to the upper arm for 2 months. Prior to this, each subject caused pigmentation of the skin using an ultraviolet light irradiator. The whitening effect was evaluated by visually observing the effect of improving skin whitening on the skin, and the results are shown in Table 8.

Whitening effect
Great Whitening effect
slightly Whitening effect
none Comparative Example 1 0 3 17 Comparative Example 2 3 8 9 Formulation Example 1 10 5 5

Claims (9)

A cosmetic composition for skin whitening comprising as an active ingredient, a quinoa fermented extract obtained by fermenting quinoa with Bacillus subtilis . The method according to claim 1,
Wherein the quinoa fermented extract is obtained by fermenting Bacillus subtilis for 5 to 10 days while maintaining a pH of 5 to 8 under a temperature condition of 28 to 42 캜. The method according to claim 1,
Wherein the quinoa fermented extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. The method according to claim 1,
The cosmetic composition may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray By weight based on the total weight of the cosmetic composition. delete delete delete delete delete