KR20210067567A - Cosmetic composition for improving skin wrinkle comprising extract of plumeria alba flower and lilium concolor var - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating skin wrinkles comprising, as an active ingredient, a mixed extract or Plumeria alba flower and Lilium concolor var or a mixed fermentation extract of Plumeria alba flower and Lilium concolor var. The cosmetic composition according to the present invention has excellent anti-aging effects and skin wrinkle improvement effects.

Description

COSMETIC COMPOSITION FOR IMPROVING SKIN WRINKLE COMPRISING EXTRACT OF PLUMERIA ALBA FLOWER AND LILIUM CONCOLOR VAR }

The present invention relates to a cosmetic composition for improving skin wrinkles containing a mixed extract of wild frangipani flower (Plumeria Alba flower) and Lilium concolor var. buschianum Bak as an active ingredient.

Recently, as women's desire for cosmetic functions has increased, high-functional products with not only cleanliness and aesthetic functions, which are the basic functions of cosmetics, but also whitening effects that show white skin like Western women, and anti-wrinkle effects, have appeared. interest is growing In addition, as the word “while” appears in society, many women who want to maintain their youth are interested in wrinkle improvement.

Wrinkles are caused by the aging of the skin with increasing age, and aging skin is the totality of natural changes associated with the aging process. Skin aging is divided into two major categories. It is divided into physiological aging, which is indicated by changes in skin function and structure related to age, and photoaging by ultraviolet rays.

Aging may occur even if the metabolic process of fibroblasts is not smooth due to the external harmful environment. Fibroblasts are present in the dermal layer of the skin and produce collagen in the extracellular matrix. Collagen is an important protein that accounts for 30% of the total weight of biological proteins and has a strong triple helix structure. The main functions are known as mechanical firmness of skin, resistance of connective tissue and support of cell adhesion. In such collagen, the function of fibroblasts is reduced due to aging and photoaging caused by UV irradiation, and the amount of collagen produced is reduced, and collagen reduction is promoted by collagenase enzyme activity that decomposes collagen. In general, the thickness of the skin decreases by about 65% compared to the age of 20 at the age of 80, and this phenomenon is known to be closely related to the formation of wrinkles on the skin.

Free radicals generated by ultraviolet rays in sunlight are highly reactive reactive oxygen species and cause cell damage and death by oxidizing or modifying vital components such as sugar, protein, DNA, and cell membrane lipids, which are cell components. In particular, it has been found to be the biggest factor causing cellular aging in the skin. A free radical is an element or molecule having one or more unpaired electrons that occurs due to oxygen reduction, magnetic flux, phagocytosis, etc., during the respiration process of a living body. It has a very short life span of several thousandths, is unstable, and is very aggressive. It exhibits properties and causes cell damage.

Currently, retinoid, adenosine, animal placenta-derived protein, betulinic acid, chlorella extract, and the like are known as wrinkle-improving cosmetics. Retinol, which is well-known, promotes collagen synthesis and inhibits elastase enzyme, but is unstable and has limited usage due to safety issues such as irritation and redness when applied to the skin. It is difficult to expect the skin wrinkle improvement effect by promoting Therefore, it is necessary to develop a new collagen synthesis promoter that is safe and effective in the living body.

In addition, aging involves not only free radicals but also an enzyme called Matrix etalloproteinase (MMP), which is a collagen degrading enzyme. That is, the synthesis and degradation of extracellular matrix, such as collagen, is appropriately regulated in vivo, but as aging progresses, collagen synthesis decreases, and the expression of matrix metalloproteinase (MMP), an enzyme that degrades collagen, is promoted, so that the skin The elasticity of the skin is reduced and wrinkles are formed. In addition, these decomposing enzymes are also activated by ultraviolet irradiation. Therefore, there is a need for the development of a substance capable of regulating MMP expression induced in the cell or inhibiting its activity.

Prior art for a cosmetic composition for wrinkle improvement using a natural product has been disclosed. Korean Patent Registration No. 10-1405429 discloses a cosmetic composition containing wild herbs such as dandelion. In addition, Korean Patent Application Laid-Open No. 2013-0136602 discloses a composition for external application for skin containing water parsley extract and fermented extract, which has excellent skin elasticity strengthening, whitening and wrinkle improvement effects.

As a result of searching for various skin physiological activities for natural plant components to improve aging, the present inventors found wild frangipani flower (Plumeria Alba flower) and Lilium concolor var. buschianum Bak among natural plant extracts. ) showed excellent antioxidant, elastase inhibitory activity, collagen synthesis promoting effect, and MMP-1 production inhibitory effect, and completed the present invention. The present invention was completed by confirming that the mixed fermented extract of Nari can more effectively improve skin wrinkles.

The above object is achieved by a cosmetic composition for improving skin wrinkles containing as an active ingredient a mixed extract of Wild Frangipani flower and S. fern frangipani or a mixed fermented extract of Wild Frangipani flower and Zinnia frangipani.

Preferably, the mixed extract of the wild Frangipani flower and the Apifolium spp. or the mixed fermented extract of the wild Frangipani flower and S. S. fern is used in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. may contain.

Also preferably, the mixed fermented extract of Wild Frangipani flower and S. oleifera maintains a pH of 5 to 8 under a temperature condition of 28 ° C. to 42 ° C. by using a solvent extract of Wild Frangipani flower and S. oleifera. It can be obtained by fermentation for 5 to 10 days.

Also preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray. It may be a formulation selected from

The present invention provides wild frangipani flower and Lilium concolor var. buschianum Bak Extract or wild frangipani flower and fermented Plumeria Alba flower and Lilium concolor var. buschianum Bak) as an active ingredient to provide a cosmetic composition that is excellent in collagen biosynthesis, MMP-1 production inhibitory effect, wrinkle improvement effect, and skin aging prevention effect.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and have the meanings as commonly understood by one of ordinary skill in the art in the relevant field of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention.

The term "about" refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprises" and "comprising" include the steps or elements presented, or groups of steps or elements, but any other step or element, or It is to be understood that a step or group of elements is not excluded.

The present invention relates to a cosmetic composition for improving skin wrinkles, comprising a mixed extract of wild frangipani flower and Apifolius or a mixed fermented extract of wild frangipani flower and Apifolium monaeus as an active ingredient.

The wild frangipani flower (Plumeria Alba flower) used in the present invention is native to the southern provinces of West India and refers to a white flower blooming from a tree of 4 to 9 m in height in the oleaceae family, and the petals are divided into 5 in a funnel shape, and the diameter is about 5 cm. .

The wild frangipani flower extract used in the present invention means an extract obtained from a flower, preferably from a blooming flower.

Lilium concolor var. buschianum Bak used in the present invention grows around sunny wetlands in the high mountains of Mt. Jiri. The height is 60~80cm, and the leaves rise up along the stem and come out thin and sharp. The roots are made of scales, and unlike the sky lily, it has thick scales. The flowers are dark yellow, 7-10 cm, rather large, and there are black spots on the petals. The fruit runs in October and has a flat, square shape, which is very different from other native Lilyaceae plants. Unlike the sky lily, it has a very different appearance because the flowers are large.

The extract used in the present invention means those obtained by extraction from various organs or parts (eg, leaves, flowers, roots, stems, etc.), preferably an extract obtained from a flower, and most preferably a flowering plant. It means that it is obtained from flowers.

In the present invention, the mixed extract of wild frangipani flower and lichen serrata is preferably mixed with wild frangipani flower and ginseng lily of the valley in a weight ratio of 1-5:1-5, more preferably 1: It is mixed in a weight ratio of 1:.

The wild frangipani flower and lindenus oleifera extract used in the present invention may be prepared using a conventional solvent under normal temperature and pressure conditions, but preferably (a) water, having 1 to 4 carbon atoms. Anhydrous or hydrous lower alcohols (e.g., methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane and a solvent extraction method using a solvent selected from the group consisting of a mixture thereof, (b) a supercritical extraction method using reduced pressure and high temperature with carbon dioxide, or (c) an ultrasonic extraction method.

In the present invention, the mixed fermented extract of Wild Frangipani flower and S. L. serrata is meant obtained by fermenting a solvent extract of Wild Frangipani flower and S. L. fern.

The mixed fermented extract of Wild Frangipani flower and S. oleifera of the present invention is a culture medium obtained by adding the solvent extract of the Wild Frangipani flower and L. fern fermenter to a normal microbial culture medium, and therein, the microorganism Inoculate and ferment before use. Specifically, 1 to 30% by weight of extracts of Wild Frangipani flower and E. L. serrata are added to the culture medium with respect to the total weight of the culture medium, and from the group consisting of yeast bacteria, lactic acid bacteria, Bacillus genus and mixtures thereof. The selected microorganism is added in an amount of 10,000 to 100,000 CFU/L. The culture temperature may be 28 ~ 42 ℃ conventional microbial culture conditions can be cultured, preferably 30 ~ 37 ℃ can be cultured. The pH is 5 to 8, and cultured for about 5 to 10 days under aerobic or usually anaerobic conditions, and then can be obtained through aging and filtration. In the culture medium used for fermentation, the amount of wild frangipani flower and ginseng extract may be used by adding 1 to 30% by weight based on the total weight of the culture medium. If the amount of wild Frangipani flower and E. fern extract is less than 1% by weight, the amount of fermented liquid by microorganisms is small and the effect is difficult to appear, and if it exceeds 30% by weight, the wild Frangipani flower and E. It does not dissolve well, so fermentation efficiency is reduced.

The fermentation strain used in the fermentation process may be selected from yeast, lactic acid bacteria, Bacillus genus, and mixtures thereof, preferably Aspergillus sp., Bacillus sp., Lactobacillus sp. .) One or more selected from the strains can be used.

An example of the Lactobacillus strain is Lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus brevis (Lactobacillus brevis), Lactobacillus buchneri (Lactobacillus buchneri), Lactobacillus bulgaricus (Lactobacillus hillaracillus), Dee (Lactobacillus hilgardii), Lactobacillus homohiochii (Lactobacillus homohiochii), Lactobacillus jensenii (Lactobacillus jensenii), Lactobacillus lactis (Lactobacillus bulgaricus), Lactobacillus casei (Lactobacillus casei), A. Sous (Lactobacillus caselactis), Lactobacillus leichmannii (Lactobacillus leichmannii), Lactobacillus casei (subspecies) casei (Lactobacillus caseisubsp. casei), Lactobacillus casei subsp. casei, Lactobacillus casei (subspecies) Pseudo plantarum (Lactobacillus casei subsp. Bacillus casei rhamnosus (Lactobacillus casei subsp. rhamnosus), Lactobacillus casei subsp. tolerans (Lactobacillus casei subsp. tolerans), Lactobacillus canenaformis (Lactobacillus catenaformis), Lactobacillus cellobiosus, Lactobacillus cellobiosus (Lactobacillus cellobiosus) Death (Lactobacillus collinoides), Lactobacillus confusus (Lactobacillus confusus), Lactobacillus coryniformis (Lactobacillus coryniformis), Lactobacillus coryniformis (subspecies) coryniformis (Lactobacillus coryniformis subsp. coryniformis) liniformis (Subspecies) Torquen's (Lactobacillus coryniformis subsp. torquens), Lactobacillus licheniformis, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus delbrueckii, Lactobacillus delbrueckii Any one or more selected from Lactobacillus fermentum), Lactobacillus fructivorans, Lactobacillus fructosus, Lactobacillus helveticus may be used, and more preferably Lactobacillus helveticus. Cus (Lactobacillus bulgaricus) is preferred.

As an example of the Aspergillus strain, any one or more selected from Aspergillus oryzae, Aspergillus niger, and Aspergillus may be used, and more preferably, Aspergillus duck agent (Aspergillus oryzae) is preferred.

An example of the Bacillus strain Bacillus acidocaldarius (Bacillus acidocaldarius), Bacillus alcalophilus (Bacillus alcalophilus), Bacillus alvei (Bacillus alvei), Bacillus anthracis (Bacillus anthracis), Bacillus badius (Bacillus badius), Bacillus brevis, Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus fastidiosus, Bacillus lentimorbus ), Bacillus lentus lentus), Bacillus licheniformis, Bacillus macerans, Bacillus macquariensis, Bacillus megaterium megaterium), Bacillus mycoides, Bacillus mycoides Bacillus pantothenticus (Bacillus pantothenticus), Bacillus pasteurii (pasteurii), Bacillus plimiksa polymyxa) , Bacillus popilliae, Bacillus pumilus (Bacillus pumilus), Bacillus sphaericus, Bacillus sphaericus, Bacillus sphaericus Rotrmophilus (Bacillus stearothermophilus), Bacillus subtilis (Bacillus subtilis), Bacillus thuringiensis (Bacillus thuringiensis) may be used any one or more selected from, preferably Bacillus subtilis (Bacillus subtilis) is preferred.

The mixed fermented extract of the wild frangipani flower and the Apifolium spp. or the mixed fermented extract of the wild frangipani flower and the fermented fern frangipani is 0.001 to 30.0% by weight, preferably 0.01 to 10.0% by weight, based on the total weight of the cosmetic composition. It is characterized in that it contains % by weight. When the content of the extract is less than 0.001% by weight, the skin improvement effect does not appear, and when it exceeds 30.0% by weight, the degree of increase in the skin improvement effect with increasing the content is insignificant, there is a problem in safety and stability of the formulation, and the economy not even an enemy Therefore, the cosmetic composition of the present invention comprising a mixed extract or a mixed fermented extract of wild frangipani flower and ginseng frangipani flower having such various functions has excellent antioxidant effect, elastase inhibitory activity, collagen biosynthesis effect, MMP-1 production It is characterized in that it has an inhibitory effect.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredient as an active ingredient, for example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. , and a carrier.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide is used as a carrier component. can be

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-Butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether as carrier components Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester may be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples according to the gist of the present invention.

Preparation Example 1 - Preparation of wild frangipani flower extract

After washing wild frangipani flowers with clean water, drying them, cut them to a certain size, put 70% (V/V) aqueous ethanol solution in the dried sample, refluxed extract 3 times for 5 hours each, and cooled, after which Whatman (Whatman) Filtered with #4 filter paper. The filtered extract was concentrated under reduced pressure at 50° C. or lower and freeze-dried.

Preparation Example 2 - Preparation of extract of ginseng lichen

After washing the flowers of the genus oleifera in clean water and drying them, cut them to a certain size, add 70% (V/V) ethanol aqueous solution to the dried sample, reflux extract 3 times for 5 hours each, and cool it, then Whatman (Whatman) Filtered with #4 filter paper. The filtered extract was concentrated under reduced pressure at 50° C. or lower and freeze-dried.

Preparation Example 3 - Preparation of a mixed extract of wild frangipani flower and ginseng frangipani

Rinse and dry the flowers of Yild Frangipani flower and the flower of Elysopharynx with clean water, cut to a certain size, and put 70% (V/V) aqueous ethanol solution into the sample mixed with a dry weight ratio of 1:1, 3 times for 5 hours each After reflux extraction and cooling, it was filtered with Whatman #4 filter paper. The filtered extract was concentrated under reduced pressure at 50° C. or lower and freeze-dried.

manufacturing example 4 - wild frangipani flower and Ginpuri sky lily Fermented extract preparation

Rinse and dry the flowers of Yild Frangipani flower and the flower of Elysopharynx with clean water, cut to a certain size, and put 70% (V/V) aqueous ethanol solution into the sample mixed with a dry weight ratio of 1:1, 3 times for 5 hours each After reflux extraction and cooling, it was filtered with Whatman #4 filter paper. The filtered extract (2L 70% EtOH) was concentrated under reduced pressure at 50° C. or less, so that the fermenting microorganisms were well cultured, the moisture content was adjusted to 70% by adding water to obtain a fermentation raw material. Then, the obtained fermentation raw material was sterilized at 121° C. for 15 minutes. For the culture of the fermentation strain, LB medium (Difco, USA) was used, and 3% by weight of Bacillus subtilis was inoculated into the medium relative to the weight of the medium, and then cultured at 37° C. at pH 7.5 at aerobic conditions for 10 days. did it Then, the culture medium was filtered using 0.25 μM filter paper, and the filtrate was aged at 4° C. for 10 days at a low temperature, followed by final filtration using 0.25 μM filter paper.

Experimental Example 1 - Measurement of DPPH radical scavenging ability

In order to measure the antioxidant effect of Preparation Examples 1 to 4, the antioxidant activity was measured using the DPPH method using L-Ascorbic acid as a comparative sample under laboratory conditions.

The DPPH method uses a free radical called DPPH (2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl) to measure antioxidant activity by reducing power. The degree of decrease in absorbance due to the reduction of DPPH by the test substance is compared with the absorbance of the blank test solution, and the free radical scavenging rate is measured at a wavelength of 560 nm. As a reagent used, 2,2-Di(4-tertoctylphenyl)-1-picrylhydrazyl free radical (Aldrich Chem. Co., MW=618.76) 0.2 mM solution is dissolved in methanol and adjusted to 100 ml. As a measurement method, 0.15 ml of 0.2 mM DPPH solution and 0.15 ml of sample solution are added to a 96-well plate, stirred quickly, and incubated at 25° C. for 10 minutes. Thereafter, the absorbance St at 560 nm is measured. For the blank test, use distilled water instead of the sample solution and measure the absorbance Bt in the same manner as above. The blank of the sample solution is operated in the same way using methanol instead of 0.2 mM DPPH solution, and absorbance Bo is measured.

The results were calculated by Equation 1, and the results are shown in Table 1. As a comparison group, L-ascorbic acid was used.

St: Absorbance at 560 nm after free radical scavenging of the sample solution

Bt: Absorbance at 560 nm after free radical scavenging of blank test solution

So: Absorbance at 560 nm before reaction when free radicals are not added to the sample solution

Bo: Absorbance at 560 nm before reaction when free radicals are not added to blank test solution

Sample name Concentration (ppm) Scavenging ability (%) Preparation Example 1 1000 35.10 ± 1.43 Preparation 2 25.78 ± 1.32 Preparation 3 40.10 ± 0.34 Preparation 4 53.90 ± 0.79 Preparation Example 1 500 23.10 ± 2.33 Preparation 2 10.50 ± 0.45 Preparation 3 30.31 ± 3.73 Preparation 4 42.76 ± 2.55 Preparation Example 1 100 19.10 ± 2.57 Preparation 2 09.03 ± 1.34 Preparation 3 25.33 ± 2.23 Preparation 4 33.31 ± 2.93

As can be seen from Table 1, a mixed extract of wild frangipani flower and ginseng frangipani according to the present invention (Preparation Example 3) and a mixed fermented extract of wild frangipani flower and ginseng hyacinth (Preparation Example 4) ), it can be seen that the free radical scavenging effect is superior to that of the wild frangipani flower extract (Preparation Example 1) or the ginseng extract (Preparation Example 2). In particular, it can be seen that the free radical scavenging effect of the mixed fermented extract (Preparation Example 4) of the wild frangipani flower and the mixed extract (Preparation Example 3) of Wild Frangipani flower and S. have.

Experimental Example 2 - Elastase (Elastase) inhibitory activity measurement

Elastase is an enzyme that breaks down elastin, an important substrate for maintaining skin elasticity in the dermis. Elastase is also a non-specific hydrolase that can degrade collagen, another important matrix protein. Therefore, the elastase inhibitor exhibits the action of improving skin wrinkles, and ursolic acid and the like are used as the elastase inhibitor. Elastase is known as an enzyme that is the main cause of wrinkles by breaking elastin, an insoluble elastic fiber protein in animal connective tissue, and breaking the network structure of the dermal tissue of the skin. In the dermal tissue of the skin, collagen and elastin related to the elasticity of the skin form a network structure. As this network structure is broken, that is, elastin is decomposed by elastase, causing the skin to sag and wrinkles, resulting in intrinsic skin aging. do. Therefore, skin aging can be suppressed by lowering the activity of elastase, an elastin degrading enzyme, which is one of the main causes of skin aging.

Using N-succinyl-(L-Ala)3-p-nitroanilide, S4760, Sigma) as a substrate, the amount of p-nitroanilide produced from the substrate was measured at 445 nm at 37°C for 20 minutes. That is, prepare each test solution to a certain concentration, take 500 μL of each test tube, and add 500 μL of a solution of porcine pancreas elastase (E1250, Sigma) 2.5 U/mL) dissolved in 2 mM tris-HCl buffer (pH 8.6), and then 50 mM as a substrate N-succinyl-(L-Ala)3-p-nitroanilide (500 μg/mL) dissolved in tris-HCl buffer (pH 8.6) was added, followed by reaction for 20 minutes. Elastane inhibitory activity was calculated according to the following Equation 2 as the absorbance decrease rate of the group with and without the addition of the sample solution.

Sample name Concentration (ppm) Elastase inhibitory activity (%) Preparation Example 1 100 10.025 Preparation 2 09.209 Preparation 3 14.129 Preparation 4 27.219 Preparation Example 1 500 14.255 Preparation 2 13.525 Preparation 3 25.022 Preparation 4 50.115 Preparation Example 1 1000 25.333 Preparation 2 20.205 Preparation 3 51.233 Preparation 4 62.229

As can be seen from Table 2, Elasta of the wild Frangipani flower and Elysium oleifera extract (Preparation Example 3) and the wild Frangipani flower and fermented extract (Preparation Example 4) according to the present invention It can be seen that the inhibitory activity effect is superior to that of the wild parinifera flower extract (Preparation Example 1) or the ginseng extract (Preparation Example 2), and in particular, the wild frangipani flower and ginseng extract (Preparation Example 3). ) It can be seen that the elastase inhibitory activity of the wild frangipani flower and the fermented extract (Preparation Example 4) of the wild frangipani flower (Preparation Example 4) is better.

Experimental Example 3 - Measurement result of collagen biosynthesis using ELISA kit

DMEM (Dulbecco's) containing 10% FBS, Penicillin (50U/ml), Streptomycin (50/ml) in a cell culture medium maintaining constant humidity at 37°C, 5% CO _{2 incubator.} Modified Eagle's Medium, Gibco, USA) was cultured, and 500 μl was dispensed into a 24 well plate at 1×10 5 cells/ml, followed by incubation for 24 hours.

Quantification of collagen type I in fibroblasts was carried out by immunosorbent assay (ELISA) using 'Procollagen Type I c-peptide EIAkit (TaKaRa, Japan)', and for 48 hours for analysis The cultured cell culture supernatant was carefully collected. First, 100 ul each of the culture supernatant cultured for 48 hours was added to a strip coated with 'Procollagen Type I c-peptide-binding antibody (antibody), and reacted at 37°C for 2 hours. After the reaction, it was washed three times using a washing buffer to remove unreacted materials, and then 100 ul of blocking buffer was added thereto, followed by reaction at 37° C. for 2 hours. After the reaction, it is washed three times using a washing buffer to remove unreacted substances, and a solution in which POD is conjugated to another antibody binding to 'Procollagen Type I c-peptide' (Antiobody-) POD conjugate solution) 100 ul each was added and reacted at 37° C. for 2 hours. After the reaction, wash 3 times using a washing buffer to remove unreacted substances, put an equal amount of substrate solution, react for 30 minutes, and then add a stop solution (1N H2SO4) and conduct the experiment. ended. The absorbance of the strip at which the experiment was completed was measured at 450 nm, and the amount of newly synthesized collagen was measured as the amount of PICP compared with the collagen standard, and the synthesis effect of collagen is shown in Table 3.

Sample name Concentration (ppm) Collagen synthesis amount (ng/2x10 ⁴ ) Preparation Example 1 200 106 ± 3.70 Preparation 2 089 ± 4.33 Preparation 3 134 ± 1.45 Preparation 4 210 ± 1.33 Preparation Example 1 100 54 ± 3.55 Preparation 2 40 ± 3.15 Preparation 3 70 ± 5.71 Preparation 4 103 ± 4.65

As can be seen in Table 3, a mixed extract of wild frangipani flower and lichen perilla according to the present invention (Preparation Example 3) and a mixed fermented extract of wild frangipani flower and ginseng lily (Preparation Example 4) It can be seen that the collagen synthesis of ) is superior to that of the wild parinid lily flower extract (Preparation Example 1) or the ginseng extract (Preparation Example 2). In particular, the collagen synthesis amount of the mixed fermented extract (Preparation Example 4) of wild frangipani flower and ginseng frangipani flower is excellent compared to the mixed extract of wild frangipani flower and ginseng extract (Preparation Example 3), so that the wrinkle improvement effect is more It can be seen that excellent

Experimental Example 4 - MMP-1 production inhibitory effect

In order to determine whether the extract obtained in Preparation Examples 1 to 4 has the effect of inhibiting the production of MMP-1, a collagen degrading enzyme, TGF-β, a substance known to have an effect of inhibiting the production of MMP-1 (10ng/ml, Roche, USA) was used as a control group to measure the effect of inhibiting the production of MMP-1. Fibroblasts, which are normal human skin cells (Korea Cell Line Bank, Korea), were inoculated in a 48-well microplate (Nunc, Denmark) to 1×10 6 cells per well, DMEM medium (Sigma, USA) and at 37° C. After culturing for 24 hours, serum-free DMEM medium added at a final concentration of 100 μg/ml of the extract obtained in Preparation Examples 1 to 4, and serum-free DMEM medium containing no extract as a control, for 48 hours further cultured. After incubation, the supernatant of each well was collected and the amount of newly synthesized MM P-1 (ng/ml) was measured using an MMP-1 assay kit (Amersham, USA), and MMP-1 according to Equation 3 below. The production inhibition rate (%) was calculated, and the results are shown in Table 4.

Sample name Concentration (ppm) MMP-1 production inhibition rate (%) Preparation Example 1 200 25.15 ± 2.24 Preparation 2 20.52 ± 2.05 Preparation 3 43.06 ± 3.25 Preparation 4 56.41 ± 3.01 Preparation Example 1 100 13.26 ± 4.02 Preparation 2 11.15 ± 4.33 Preparation 3 23.37 ± 3.12 Preparation 4 42.26 ± 3.02

As can be seen in Table 4 above, a mixed extract of wild Frangipani flower and Apifolium nigra according to the present invention (Preparation Example 3) and a mixed fermented extract of Wild Frangipani flower and E. It can be seen that the inhibition rate of MMP-1 production of 4) is superior to that of the wild frangipani flower extract (Preparation Example 1) or the wild paringian leaf extract (Preparation Example 2). In particular, it can be seen that the MMP-1 production inhibition rate of the mixed fermented extract (Preparation Example 4) of the wild frangipani flower and the mixed extract (Preparation Example 3) of the wild Frangipani flower and S. have.

Formulation Example 1 - Cream

A prescription example of a cream in a cosmetic containing a mixed extract of wild frangipani flower and Apifolius or wild frangipani flower and a mixed fermented extract of wild frangipani flower is shown in Table 5 below.

Classification (Unit: Weight %) Prescription Example 1 Prescription Example 2 Wild Frangipani flower and ginseng frangipani extract (Preparation Example 3)
Wild Frangipani flower and fermented extract of ginseng frangipani (Preparation Example 4)
lipophilic monostearate glycerin
cetearyl alcohol
stearic acid
beeswax
Polysorbate 60
Sorbitan Stearate
hydrogenated vegetable oil
squalane
mineral oil
trioctanoin
dimethicone
Sodium Magnesium Silicate
glycerin
betaine
triethanolamine
Sodium Hyaluronate
preservatives, fragrances, colors
Distilled water 3.0
-
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
remaining amount -
3.0
2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
remaining amount Sum 100 100

Formulation example 2 - lotion

A formulation example of a lotion among cosmetics containing a mixed extract of wild frangipani flower and Apifolius or a mixed fermented extract of wild frangipani flower and Apifolium spp. is shown in Table 6 below.

Classification (unit: wt%) Prescription Example 3 Prescription Example 4 Yield Frangipani flower and ginseng frangipani extract (Preparation Example 3)
Fermented extract of Yelde Frangipani flower and ginseng lichen (Preparation Example 4)
glycerin
1.3-Butylene Glycol
PG 1500
allantoin
DL-Panthenol
EDTA-2Na
Benzophenone-9
Sodium Hyaluronate
ethanol
Octic dodeces-16
Polysorbate 20
preservatives, fragrances, colors
Distilled water 3.0
-
5.0
3.0
1.0
0.1
0.3
0.02
0.04
5.0
10.0
0.2
0.2
a very small amount
remaining amount -
3.0
5.0
3.0
1.0
0.1
0.3
0.02
0.04
5.0
10.0
0.2
0.2
a very small amount
remaining amount Sum 100 100

Formulation Example 3 - Essence

Prescription examples of essences in cosmetics containing wild frangipani flower and ginseng lily extract or wild frangipani flower and fermented fermented extract of fermented frangipani are shown in Table 7 below.

Classification (unit: wt%) Prescription Example 5 Prescription Example 6 Wild Frangipani flower and ginseng frangipani extract (Preparation Example 3)
Wild Frangipani flower and fermented extract of ginseng frangipani (Preparation Example 4)
glycerin
betaine
Fiji 1500
allantoin
DL-Panthenol
E.D.T.A-2Na
Benzophenone - 9
Hydroxyethyl Cellulose
Sodium Hyaluronate
Carboxyvinyl Polymer
triethanolamine
Octyldodecanol
Octyldodeces -16
ethanol
preservatives, fragrances, colors
Distilled water 3.0
-
10.0
5.0
2.0
0.1
0.3
0.02
0.04
0.1
8.0
0.2
0.18
0.3
0.4
6.0
a very small amount
remaining amount -
3.0
10.0
5.0
2.0
0.1
0.3
0.02
0.04
0.1
8.0
0.2
0.18
0.3
0.4
6.0
a very small amount
remaining amount Sum 100 100

Comparative Formulation Example 1 - Cream

Prescription examples of creams that do not contain wild frangipani flower and ginseng extract or wild frangipani flower and fermented extract of fermented algae are shown in Table 8 below.

Classification (unit: wt%) Prescription Example 7 lipophilic monostearate glycerin
cetearyl alcohol
stearic acid
beeswax
Polysorbate 60
Sorbitan Stearate
hydrogenated vegetable oil
squalane
mineral oil
trioctanoin
dimethicone
Sodium Magnesium Silicate
glycerin
betaine
triethanolamine
Sodium Hyaluronate
preservatives, fragrances, colors
Distilled water 2.0
2.2
1.5
1.0
1.5
0.6
1.0
3.0
5.0
5.0
1.0
0.1
5.0
3.0
1.0
4.0
a very small amount
remaining amount Sum 100

Experimental example 5 - Evaluation of skin wrinkle improvement effect

The wrinkle-improving effect of a cosmetic containing a mixed extract of wild frangipani flower and Apifolius or a mixed fermented extract of wild frangipani flower and Apifolius according to the present invention was evaluated through an actual use test. Prescription Example 1 cream containing 3% (v/v) of a mixed extract of wild frangipani flower and lichen serrata, and 3% (v/v) of a mixed fermented extract of wild frangipani flower and lichen. Formula 2 cream containing v), and formulation example 7 cream of Comparative Formulation Example 1 in which the extract was replaced with purified water was evaluated. Thirty women aged 30-40 years were randomly divided into two groups, and each cream was applied twice a day in the morning and in the evening, and an appropriate amount of the cream was applied around the eyes continuously for 2 months. The wrinkle improvement effect of each subject was evaluated through visual observation. The experimental results are shown in Table 9 below.

division Excellent wrinkle improvement effect Slight wrinkle improvement effect No wrinkle improvement effect Prescription Example 1 15 12 3 Prescription Example 2 20 8 2 Prescription Example 7 4 7 19

As can be seen in Table 9, a mixed extract of the present invention of wild frangipani flower and Apis serrata (Formulation Example 1) or a mixed fermented extract of a wild frangipani flower and Amygdala (Formulation Example 2) The cosmetic containing the wrinkle-improving effect is superior to that of the cosmetic that does not contain the mixed extract (Formulation Example 7), and the Wild Frangipani flower compared to the mixed extract of Wild Frangipani flower and Zinnia flower (Formulation Example 1). And it can be seen that the cosmetic containing the mixed fermented extract (Prescription Example 2) of Jinpurii serrata has a higher wrinkle improvement effect, and skin irritation can be observed in the subjects of the part where the cosmetic of the present invention is applied to the skin. couldn't

Claims (4)

A cosmetic composition for improving skin wrinkles, comprising a mixed extract of wild frangipani flower and Apifolius or a mixed fermented extract of wild frangipani flower and A. According to claim 1,
The mixed extract of the wild Frangipani flower and the Apifolius or the mixed fermented extract of the Wild Frangipani flower and the Alyssalea is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition, the skin A cosmetic composition for improving wrinkles. According to claim 1,
The mixed fermented extract of the wild Frangipani flower and the Apifolium spp. is a solvent extract of a wild Frangipani flower and a Ginseng Frangipani flower while maintaining a pH of 5 to 8 under a temperature condition of 28 ° C. to 42 ° C., 5 to 10 A cosmetic composition for improving skin wrinkles, which is obtained by daily fermentation. According to claim 1,
The cosmetic composition comprises a formulation selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. Having, a cosmetic composition for improving skin wrinkles.