KR101150556B1 - Composition for Preventing Hair Loss and Promoting Hair Growth - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss and promoting hair growth, and more specifically, iris root extract, ginseng extract, 칡 extract, nettle extract, sewage extract, ginseng extract, medicinal extract, centella extract, sancho extract, machihyun extract and It relates to a composition for preventing hair loss and promoting hair growth comprising a bokyong extract.
Hair loss prevention and hair growth promoting composition according to the present invention is made of a natural product has no side effects to the human body, as well as smooth blood circulation to the hair root cells, scalp protection and hair nourishment function has excellent effects in preventing hair loss and promoting hair growth have.

Description

Composition for Preventing Hair Loss and Promoting Hair Growth

The present invention relates to a composition for preventing hair loss and promoting hair growth that does not contain side effects to the human body containing natural herbal extracts.

Hair protects and protects the body from external stimuli such as buffering and external stimuli such as ultraviolet rays, temperature, and friction, and absorbs heavy metals such as arsenic, mercury, and zinc that are unnecessary to the body and releases them to the body. It is an important aesthetic factor in the expression of external images.

In modern society, physical rhythm is caused by various causes such as physical, mental, genetic, and environmental factors such as side effects from stress, environmental pollution, drug addiction and misuse, perm, dyeing, use of hair dressings and genetic factors. It is broken and unbalanced, not only the scalp but also hair follicles are greatly affected, resulting in various forms such as hair loss as well as dry or sensitive hair loss.

Currently, the cause of hair loss is caused by a number of factors. First, male hormone testosterone, DHT (5 alpha-dihydrotestosterone), is highly active in the hair follicle due to the overproduction of hormones by genetic factors. DHT binds to androgen receptors to slow down the hair follicle protein synthesis, shorten hair follicle growth and shrink hair follicles to cause hair loss, or to cover hair follicles and nipples as an abnormal blood circulation supplied to the nipples If the amount of peripheral capillaries in the blood flow decreases and the nutrients are insufficient in the area of the papilla and basal cells, metabolism is reduced, causing hair growth abnormalities, or if dandruff occurs due to a decrease in physiological function of the scalp, Hair loss due to itching and inflammation of the scalp due to clogging and bacterial propagation Or hair loss due to abnormal blood growth caused by decreased blood flow in the peripheral blood vessels of the subcutaneous tissue of the head due to tension of the scalp, or other malnutrition, adverse effects caused by drugs, heredity, stress, environmental factors, etc. Hair loss is known to be caused by various factors.

As much attention is currently focused on hair care, researches and inventions on cosmetic compositions prepared using natural extracts (medicinal herb extracts) with little irritation and fewer side effects to prevent the occurrence of hair loss are actively progressed. As a patent application for hair loss prevention, hair growth, and hair growth promoting composition, hair loss prevention composition containing herbal extracts containing thorny skin, pine needles, pine flower, gyoza, fresh sulfur, and hair loss (Korea Patent Publication No. 10-2009-0051586), There are a number of patent applications such as hair loss prevention compositions (Korean Patent Application Publication No. 10-2005-0014473) using natural products, such as hair growth promoting compositions having a bactericidal function containing Baekduong, Daesan, and golden extracts. Thanks to such active research, various cosmetic compositions such as hair lotion, hair shampoo, hair treatment, hair rinse and the like for preventing hair loss are widely distributed and used in the market, but conventional cosmetic compositions commonly distributed are substantially There were no products with satisfactory effects on dandruff prevention and hair loss prevention, and there were problems that caused various side effects or failed to form healthy hair growth.

In addition, since the product of the composition used for the scalp or hair is frequently used for a long time, it should not show side effects such as irritation or damage, and existing commercially available products often cause side effects on the scalp because the main ingredient is a chemical. many. Recently, hair loss treatments such as minoxidil and propecia have been developed, and anti-hair loss effects are expected. However, the application of such hair loss treatments causes unexpected side effects such as decreased sexual function, allergies, depression, and local inflammation due to imbalances in improvement of the body. The risk of application is increasing.

The inventors of the present invention, while studying a composition for preventing hair loss and promoting hair growth, the composition containing natural products such as 칡, iris root, etc. without side effects, as well as blood circulation to the hair root cells, scalp protection, hair nutrition supply, etc. It has been confirmed that the systematic action has the effect of preventing hair loss and promoting hair growth, and completed the present invention.

An object of the present invention is to provide a composition for the prevention of hair loss and hair growth promoting composition having a natural extract does not have any side effects to the human body, excellent hair growth effect.

In order to achieve the above object, the present invention is a medicinal herb extract extract consisting of iris root extract, ginseng extract, 칡 extract, nettle extract, sewage extract, red ginseng extract, medicinal herbs extract, centella extract, sancho extract, machihyeon extract and bokyeong extract It provides a composition for preventing hair loss and promoting hair growth comprising a.

According to one embodiment of the present invention, the iris root extract, ginseng extract, 칡 extract, nettle extract, sewage extract, gosam extract, medicinal extract, centella extract, sancho extract, machihyeon extract and Bokyeong extract each 0.5 ~ 5 parts by weight , 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5 It may be from 5 parts by weight and 0.5 to 5 parts by weight.

In another aspect, the present invention, (a) water iris root extract, ginseng extract, 칡 extract, nettle extract, sewage extract, ginseng extract, medicinal extract, centella extract, sancho extract, machihyeon extract and bokyeong extract each 0.5 ~ 5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight Part, adding 0.5 to 5 parts by weight and 0.5 to 5 parts by weight; And (b) heating the mixture at 80 to 120 for 8 to 48 hours to obtain a hydrothermal extract to provide a method for preparing a composition for preventing hair loss and promoting hair growth.

In another aspect, the present invention, (a) adding irises, ginseng, 칡, nettle, sewage, ginseng, medicinal herbs, centella, sancho, machi, and bokyeong to water, each mixture from 80 to 120 to 8 to Heating for 48 hours to obtain each hot water extract; And (b) 0.5 to 5 parts by weight of each iris root extract, ginseng extract, 추출물 extract, nettle extract, sewage extract, ginseng extract, medicinal extract, centella extract, sancho extract, machihyun extract, and bokyeong extract, respectively. 5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight And 0.5 to 5 parts by weight of the mixture to provide a method for producing a composition for preventing hair loss and promoting hair growth comprising the step of obtaining a mixed extract.

Hair loss prevention and hair growth promoting composition according to the present invention is made of a natural product has no side effects to the human body, as well as smooth blood circulation to the hair root cells, scalp protection and hair nourishment function has excellent effects in preventing hair loss and promoting hair growth have.

1 and 2 are graphs showing the cell proliferation effect of human skin cells to confirm the cytotoxicity of the composition for preventing hair loss and promoting hair growth according to the present invention.
Figure 3 is a photograph showing the results of animal hair test to evaluate the hair growth of the composition for preventing hair loss and promoting hair growth according to the present invention.

The present invention relates to a composition for preventing hair loss and promoting hair growth, as an active ingredient, iris root extract, ginseng extract, 칡 extract, nettle extract, sewage extract, red ginseng extract, medicinal extract, centella extract, sancho extract, machihyeon extract and bokyeong extract The present invention relates to a composition for preventing hair loss and promoting hair growth, including natural ingredients, including the like.

In accordance with one aspect of the present invention, hair loss prevention comprising a medicinal herb extract, ginseng extract, ginseng extract, nettle extract, nettle extract, sewage extract, red ginseng extract, medicinal extract, centella extract, sancho extract, machihyun extract, and bokyeong extract And a composition for promoting hair growth.

Specifically, the herbal medicine mixture extract in the composition of the present invention, iris root extract, ginseng extract, 칡 extract, nettle extract, sewage extract, red ginseng extract, medicinal extract, centella extract, sancho extract, machihyeon extract and bokyeong extract 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, It may be characterized by containing 0.5 to 5 parts by weight, 0.5 to 5 parts by weight and 0.5 to 5 parts by weight. Here, each of the parts by weight represents the content ratio of the extracts, these content ratios may be contained in various ratios as appropriate within the range, not necessarily the same amount, and may be included in the preparation of the mixed extract, such as purified water in addition to the extracts The ingredients may additionally be contained.

If the content of each extract is less than the predetermined content range, hair loss prevention and hair growth promoting effects are insignificant, and if the content exceeds a predetermined range by weight, the formulation and stability may be adversely affected.

In this invention, it is preferable that the said herbal medicine mixed extract contains 0.1-10 weight part with respect to 100 weight part of whole compositions of this invention. If the content is less than 0.1 part by weight, the hair loss prevention and hair growth promoting effect and effect is insignificant, and if it exceeds 10 parts by weight, there is a problem in stabilizing the formulation.

In the present invention, the iris root extract, ginseng extract, 칡 extract, nettle extract, sewage extract, gosam extract, medicinal extract, centella extract, sancho extract, machihyeon extract and bokyeong extract can be obtained by hot water extraction or ethanol extract and For example, using water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, a mixed solvent of the lower alcohol with water, acetone, ethyl acetate, chloroform or 1,3-butylene glycol, or the like It can also be obtained by other known extraction methods.

Hereinafter, each natural product component contained in the composition of the present invention will be described in detail.

Iris is used as a swelling, toothache, typhoon, improvement treatment, and sensitizer, soothing agent, and supplement in one shot. Leaves from the stem are used as fragrance. Iris (Acorus calamus var. Angustatus) is a perennial herb belonging to the Araceae, which is scented and grows in ponds, waterways and wetlands. Underground stems have many nodes and beard roots.

Ginseng (Panax ginseng) is the most well-known nutrient tonic in both East and West, and its composition and pharmacological physiological studies have been conducted to explain the mysterious effects by modern scientific methods. The benefits of ginseng include aging inhibition, arteriosclerosis prevention, hyperlipidemia, diabetes improvement, hypertension, liver function enhancement, antioxidant effect, antistress effect, immunity enhancement, thrombus improvement, brain function improvement, brain cell protection, anticancer effect, etc. Maintain homeostasis of human body and make all pathologies to normal state. Recently, ginsenoside Rg3, which contains only trace amounts of red ginseng, has a vasorelaxant effect [J. Nat. Prod. 63, 1702 (2000)], platelet aggregation inhibitory activity [Bio. Pharm. Bull. 21, 79 (1998), Korean J. Ginseng Sci. 21, 132 (1997)], and neuronal cell protective action [J. Neuroscience Res. 53, 426 (1998), Neuro Report 9, 226 (1998)], and ginsenosides Rg3, Rg5, Rh2, and Rh1 have anticancer activity. Has been reported [Jpn. J. cancer Res. 87, 357 (1996), J. cancer Res. Clin. Oncol. 120, 24 (1993), Anticancer Res. 17, 1067 (1997), Cancer Letters 150, 41 (2000), Dietary Anticarcinogenesis and Antimutagenesis 274 (2000).

The other names of nettles (Urtica laetevirens Maxim.) Are persimmons, stinging nettles, perennial plants, 50-150 cm high, with stems in squares, with egg-shaped leaves 2.5-5 cm wide. There are nettles on stems and leaves. It grows in wet roads, streams and foothills around the country. The leaves are dried in the shade after summer. The components of the nettle are irritating acidic substances consisting of some formic acid, acetic acid and butyric acid. One gram of dry nettle of fine leaf nettles contains 3 mg of histamine. Vitamin K (400 units per gram of leaves), glycoside urticin, tannin, formic acid, ascorbic acid (0.1-0.6%), vitamin B2, pantothenic acid, protoporphyrin C34 H34 O4 N4, coporporphyrin C36 H38 O8 N4, cytosterol, histamine C5 H9 N3 (0.05 mg per gram), lecithin. In addition, 14-50 mg% of carotenoids β-carotene C40 H56, xanthophyll C40 H56 O2, xanthophyllepoxide C40 H56 O3, and violaxanthin C40 H58 O4 are contained. In addition, chlorophyll is 2-5%. Chlorophyll in nettles has the advantage of being a concern. Rainforest medicine and flow extract of the leaves of the nettle increases the coagulation of blood, increases the content of hemoglobin and the number of red blood cells. In addition, the tension of uterine smooth muscle is increased, the contraction is hard, and there is blood vessel contraction. These active ingredients are considered vitamin K and chlorophyll. Chlorophyll promotes metabolism, increases tension in the intestines, heart, blood line and uterus, and speeds up regeneration and epithelialization of wound tissue.

Cinnanchum silfordii Hemsley is a perennial vine herb that belongs to Yeowi-gu and is recognized for its weakness compared to wild ginseng. Sewage looks like sweet potatoes, but slightly smaller, and when ginseng is precious, instead of ginseng, it is used as a substitute for sorghum, or because it is less contraindicated and more digestible than sorghum. Sewage acts as a function of donkey and succinct amber to produce blood and removes inflammation, boils and swelling. In addition to sputum and phlegm, it treats postpartum disease, cold and lobular treatment of women There is a function to strengthen the musculature. When used as a life to communicate the energy to facilitate the communication of faeces and detoxification action, and steaming properly or boiled and then dried to write the liver and gods to create blood courses. For the purpose of blackening hair, black beans and black sesame seeds are recommended for use.

Ginseng is a perennial herb, 100cm high. Ginseng refers to the root and is harvested in spring and autumn. Roots barely stripped from the bark of the thief's cane Sophorae flavescens Ait. Sopocarpine is contained, and flavonoids include xanthohumol, isoxanthohumol and camphorol. Main effects include diuretic and antibacterial effects. Ginseng extract has a strong antimicrobial activity against Propionibacterium acnes, which causes acne. It also inhibits the development of various skin fungi at 8% concentration. Sophoraflavone G has antimicrobial activity against Porphyromanas, Prevotella, Actinobacillus and Capnocytopaga. (+)-matrine and (+)-oxymatrine have central inhibitory effects, such as lowering body temperature, and glutamic acid blocking on neuromuscular specimens. (+)-Matrine also significantly reduces ear inflammation caused by croton oil.

The medicinal herb is dissolved in the whole Swertia japonica Makino herb. It is called senburi extract in Japan, medicinal name in Korea, and bitter grass in gentian name. Glycolic extract is a native drug widely used in Japan for abdominal pain, skin infection, hookworm and cold. Common medicinal extracts include 2-4% of swetitiamarin, sertianolin, erythrocentaurin, swetitianol, oleanolic acid and genitianine. It consists of. Glycosides, including swetitiamarin, are known as glycosides and are commonly derived from plants and animals. These can be used to increase skin temperature and promote hair growth, such as cantharide and pepper tincture. Is reported.

Centella asiatica is a perennial herb of the dicotyledonous cultivars, an anhydraceae, known for its effectiveness in antipyretic, dentifrice or musculoskeletal and baekdae. Glycosides or derivatives thereof, which are isolated from the centella, are known to be effective in skin ulcers, extensive trauma treatment, skin veins, ulcerative ulcers, erosive skin ulcers, and alopecia areata. In addition, a new material for the treatment of dementia has recently been synthesized therefrom. It contains very useful pharmacological ingredients such as extracts of Centella asiaticoside, madecassoside, asiatic acid and madecassic acid to treat skin diseases and wounds. It is excellent for anti-dementia, asthma, headache, gonorrhea, syphilis, skin disease, leprosy and gastrointestinal diseases, and is used as a raw material for the treatment of diseases.

Sancho is Zanhosylum piperitum DC. Or other seeds of the same genus (Tropaceae Rutaceae) as much as possible. Sanshoolfb and sanshoamid, which are contained in the sancho, are microscopic substances with local paralysis action. They have insecticidal action and detoxification for fish poisoning. a-sanshool and β-sanshool have almost the same pesticidal and antimicrobial activity.

Portulaca oleracea means perennial herb and purslane. The gusset string is 15 to 30 cm in height, reddish red, and many branches are spread obliquely to the side. The root is white, but when peeled by hand, it becomes red like a main stem. The leaves face each other and are wedge-shaped ovals, and are named Machihyeon because they resemble the teeth of a horse. Flowers bloom in May-August, yellow flowers bloom at the end of branches, without peduncles, bisexual, blooms in the morning, and grows in midday. In the West, it is used as a ingredient of salad along with lettuce, and in Korea, it is mainly used for food or medicinal purposes such as herbs.

Poria cocos is a fungus with a fungus called Democratic Mushrooms. All year round, they live in tree roots such as pine trees in the ground. Fruiting body is pre-batch, without making mushroom cap, whole white, dense tube. The tube is 2 ~ 20 mm long, the hole is circular or polygonal, and the hole edge is serrated. The flesh is broiled or white. Mr. Hall is bent slightly in the shape of a cylinder and has pointed, flat and colorless ends. Fungal nuclei are 10-30 cm in the roots of pine trees, rounded or elongated or lumpy. The surface of the mushroom shade is reddish brown, light brown, and blackish brown. The flesh is white and gradually turns pink. The white ones are called baekbokyeong and the red ones are called bokbokyeong. In addition, the roots of pine trees penetrated into the ghost are called ghost gods. All herbal medicines are effective in tonic, diuretic and soothing, so they are used for kidney disease, cystitis and urethritis.

The hair loss prevention and hair growth promoting composition of the present invention may be added to the base component that can be blended in general scalp and hair external preparations as needed. Specific examples include dissolution aids, surfactants, moisturizers, thickeners, pH adjusters, preservatives, antioxidants, metal ion sequestrants, fungicides, anti-inflammatory agents, antimicrobial agents, solvents, colorants, or flavoring agents.

Here, the dissolution aid may be specifically isopropyl myristate, polyethylene glycol, medium chain fatty acid triglycerides, hydrocarbons, glycols and the like.

Among the surfactants, anionic surfactants are specifically ammonium lauryl sulfosuccinate, ammonium lauryl sulfate, sodium cocoyl isionate, sodium lauryl isceionate, sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium Lauryl ether sulfate (1 to 3 ethylene oxide) and the like. Among the surfactants, nonionic surfactants may specifically be polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene, cured castor oil derivatives, fatty acid diethanolamides, glyceryl stearate, and the like. The cationic surfactant in the surfactant may specifically be a tertiary aliphatic amine salt, alkyltrimethylammonium chloride, dialkyldimethylammonium chloride, or the like. Among the surfactants, the amphoteric surfactant may be specifically betaine, amidebetaine type, sulfobetaine type, steartrimonium chloride, or the like.

Moisturizing agent may be specifically glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol and the like.

The thickener may specifically be a water-soluble high molecular compound such as methyl cellulose, hydroxyethyl cellulose, carrageenan, carboxymethyl cellulose, hydroxymethyl cellulose and the like.

The pH adjusting agent may specifically be citric acid, sodium hydroxide, triethanolamine, sodium citrate, phosphoric acid, sodium phosphate, lactic acid and the like.

Preservatives may specifically be benzoic acid, paraoxybenzoic acid esters, mixtures of methylchloroisothiazolinone, phenoxyethanol, DMDM hydantoin and the like.

Antioxidants may specifically be dibutylhydroxytoluene, ascorbic acid and the like.

Specifically, the metal ion blocking agent may be ethylenediaminetetraacetate acetate, ethylenediaminetetraacetate tetrasodium, or the like.

Fungicides may specifically be chlorhexidine gluconate, quaternary ammonium salts, pyroxtonolamines, zinc pyrithione suspensions, iodopropynyl butylcarbamate, salicylic acid and the like.

Anti-inflammatory agents may specifically be monoammonium glycyrrhinate, dipotassium glycyrizine, stearylglycirizinate, chamomile, alpha bisabolol, allantoin and mixtures thereof and the like.

Antimicrobial agents are specifically phenoxyethanol, chlorhexidine, chlorhexidine gluconate, pyroxtonolamine, ketoconazole, arnica extract, iodopropynylbutylcarbamate, benzalkonium chloride, benzenetonium chloride, benzoic acid and salts thereof, benzyl alcohol, lavender, rosemary , Salicylic acid, triclocarban, zinc pyrithione suspensions and mixtures thereof and the like.

The solvent may be specifically ethanol, purified water, Tween 20, cyclomethicone, mineral oil, dimethicone, and the like.

Colorants and flavoring agents can use the bases used in general scalp and hair preparations.

Hair loss prevention and hair growth promoting composition of the present invention can be prepared in all formulations of cosmetic compositions, pharmaceutical compositions that can be applied to the scalp and hair, such as liquid, cream, paste, or solid phase, conventional additives Shampoos, hair rinses, hair tonics, hair gels, hair lotions, hair packs, hair sprays, hair mousses, hair treatments, hair dyes, hair conditioners, mixtures thereof, such as shampoos and rinses, It can be prepared in a composition such as a mixed type of rinse and treatment, liquid hair repellent type, and also includes an aerosol type of these formulations. The hair loss prevention and hair growth promoting composition is preferably used in a method of transdermal administration such as direct application or spraying on the scalp or hair.

In another aspect, the present invention (a) 0.5 to 5 weight of iris root extract, ginseng extract, 칡 extract, nettle extract, sewage extract, ginseng extract, medicinal extract, centella extract, sancho extract, machihyeon extract and bokyeong extract, respectively Part, 0.5 to 5 parts by weight, 0.5 to 5 parts by weight, 0.5 to 5 parts by weight, 0.5 to 5 parts by weight, 0.5 to 5 parts by weight, 0.5 to 5 parts by weight, 0.5 to 5 parts by weight, 0.5 to 5 parts by weight, Adding 0.5-5 parts by weight and 0.5-5 parts by weight; And (b) heating the mixture at 80 to 120 for 8 to 48 hours to obtain a hot water extract.

In another aspect, the present invention, (a) adding irises, ginseng, 칡, nettle, sewage, ginseng, medicinal herbs, centella, sancho, machi, and bokyeong to water, each mixture from 80 to 120 to 8 to Heating for 48 hours to obtain each hydrothermal extract; And (b) 0.5 to 5 parts by weight of each iris root extract, ginseng extract, 추출물 extract, nettle extract, sewage extract, ginseng extract, medicinal extract, centella extract, sancho extract, machihyun extract, and bokyeong extract, respectively. 5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight And a method for producing a composition for preventing hair loss and promoting hair growth comprising mixing 0.5 to 5 parts by weight to obtain a mixed extract.

In the manufacturing method, hot water extract by mixing irises, ginseng, 칡, nettle, sewage, red ginseng, medicinal herbs, centella, sancho, machi and bokyeong, or irises, ginseng, 칡, nettle, sewage, ginseng, medicinal herbs, centella , Sancho, Machihyeon and Fukryeong each by hot water extraction can be mixed to produce the mixed extract.

The weight ratio of each of the natural products is iris powder root extract, ginseng extract, 칡 extract, nettle extract, sewage extract, red ginseng extract, medicinal extract, centella extract, Sancho extract, Machihyeon extract and Bokryeong extract are each 0.5 ~ 5 parts by weight, 0.5 ~ 5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight Parts and 0.5 to 5 parts by weight. These ratios do not necessarily contain the same amount, and may be contained in various ratios within the above ranges.

In addition, the composition for preventing hair loss and promoting hair growth of the present invention may be in the form of a pharmaceutical composition. In the case of the pharmaceutical composition, it may be prepared by further containing a pharmaceutically suitable and physiologically acceptable adjuvant, wherein the adjuvant includes a solvent, a disintegrant, a sweetener, a binder, a coating agent, an expanding agent, a lubricant, a lubricant Solubilizers, such as an agent or a flavoring agent, can be used. In addition, in addition to the active ingredient described above for proper administration, it may be formulated to include one or more additional pharmaceutically acceptable carriers. Here, the pharmaceutically acceptable carrier may be used in combination with saline solution, sterile water, Ringer's solution, buffered saline solution, dextrose solution, maltodextrin solution, glycerol, ethanol and one or more of these components, if necessary, antioxidants, Other conventional additives such as buffers and bacteriostatic agents can be added.

In addition, the pharmaceutical composition for preventing hair loss and promoting hair growth may be formulated into injectable formulations such as aqueous solutions, suspensions, emulsions, pills, capsules, granules or tablets by additionally adding diluents, dispersants, surfactants, binders and lubricants. Can be. In the usual manner via the intravenous, intraarterial, intraperitoneal, intramuscular, intraarterial, intraperitoneal, sternum, transdermal, nasal, inhalation, topical, rectal, oral, intraocular or intradermal routes May be administered.

The pharmaceutical composition or cosmetic composition is a solvent of the iris root extract, ginseng extract, 풀 extract, nettle extract, sewage extract, red ginseng extract, medicinal extract, centella extract, sancho extract, machihyeon extract and bokyeong extract of the present invention It may be provided in a form containing the concentration to the extent that the effect.

The amount of the composition of the present invention can be appropriately adjusted according to individual differences, dosage forms, and forms such as age, degree of lesion, and the composition of the present invention is useful as a cosmetic, medicine, or quasi-drug for improving the scalp and hair condition. Can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Preparation of a mixed extract in a composition for preventing hair loss and promoting hair growth of the present invention

In order to obtain 10 L of the herbal mixed extract of the composition for preventing hair loss and promoting hair loss of the present invention, 100 g of iris root, 100 g of ginseng, 100 g of ginseng, 100 g of nettle, 100 g of sewage, 100 g of ginseng, 100 g of sugar , 100 g of Centella asiatica, 100 g of Sancho, 100 g of Machi Prefecture and 100 g of Bokryeong were heated to 80-120 for 8-48 hours using 20 L of purified water in a boiling water distillation unit to obtain a hydrothermal extract. For the preparation of the mixed extract, using the hot water extract thus obtained, it was prepared in the ratio of the composition disclosed in Table 1 and Table 2.

ingredient
(weight%) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Iris root
extract 0.5 - - - - - - Ginseng extract - 0.5 - - - - - 칡 extract - - 0.5 - - - - Nettle extract - - - 0.5 - - - Sewao Extract - - - - 0.5 - - Ginseng extract - - - - - 0.5 - Sugar extract - - - - - - 0.5 Centella extract - - - - - - - Sancho Extract - - - - - - - Machi Prefecture Extract - - - - - - - Bokryeong Extract - - - - - - - ethanol 50 50 50 50 50 50 50 Purified water balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100)

ingredient
(weight%) Comparative Example 8 Comparative Example 9 Comparative example
10 Comparative example
11 Comparative Example 12 Comparative example
13 Experimental Example 1 of the Invention Iris root
extract - - - - 0.5 - 0.5 Ginseng extract - - - - 0.5 - 0.5 칡 extract - - - - 0.5 - 0.5 Nettle extract - - - - 0.5 - 0.5 Sewao Extract - - - - 0.5 - 0.5 Ginseng extract - - - - - 0.5 0.5 Sugar extract - - - - - 0.5 0.5 Centella extract 0.5 - - - - 0.5 0.5 Sancho Extract - 0.5 - - - 0.5 0.5 Machi Prefecture Extract - - 0.5 - - 0.5 0.5 Bokryeong Extract - - - 0.5 - 0.5 0.5 ethanol 50 50 50 50 50 50 50 Purified water balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100)

The results of mixing the irises, ginseng, 칡, nettle, sewage, ginseng, medicinal herbs, centella, sancho, machihyeon, and bokyeong at different ratios within a predetermined range, not the same ratio, are also the same as or similar to the following experimental results. It was confirmed that an excellent effect appeared.

In addition, the iris root, ginseng, 칡, nettle, sewage, red ginseng, medicinal herbs, centella, sancho, machihyeon and Bokyeong each separately mixed in water and extracted in the same manner as above, and then each extract is mixed in the same content ratio Even in the case of preparation, the same or similar level to the results of the following experiments showed excellent effects.

Experimental Example  1: Cytotoxicity test of the composition according to the invention

(1) Human keratinocyte cell culture

In order to examine the cytotoxicity of the composition according to the present invention, the human keratinocyte HaCaT cell line (Cell Lines Service, Germany) was cultured in 37 ℃, 5%, CO ₂ incubator. When the degree of culture as much as 85 to 90% of the culture vessel was shown, the cells were detached by trypsin treatment, counted, and then subcultured at 5 × 10 ³ cells / cm ² . The culture of the cells was performed with DMEM (Dubelcco'S Modified Eagle) supplemented with 10% Fetal Bovine Serum (FBS, GIBCO, Cat.No. 26140-079, USA) and 100 U / ml penicillin and 100 / ml streptomycin. Medium, GIBCO, Cat. No. 11995-065, USA). 75 T-flask (NUNC, Cat No. 156499, Danmark) was used for subculture, and 24 well plate (NUNC, Cat. No. 142475, Danmark) was used for cytotoxicity test.

(2) cytotoxicity test

MTT assay is a method of measuring the cytotoxicity of a test substance by finally forming a pigment product called formazan by using the dehydrogenase action of mitochondria, which is active only in living cells. In order to confirm that each of the compositions prepared in Example 1 can show an improvement effect without skin irritation even after long-term use, the presence or absence of cytotoxicity against human keratinocytes was tested.

Human keratinocytes cultured in the above were counted using a heamacytometer at 5 × 10 ³ cells / well in 24 well plates and then aliquoted. When incubated in DMEM containing 10% FBS for 48 hours and then cultured by 25-30% of the surface area of the culture vessel, 10% of the composition of Comparative Examples 1 to 13 was contained, and the composition of Experiment 1 of the present invention was Incubated for 24 hours further by replacing with 10% ~ 0.1% FBS-free DMEM.

After incubation, 50 μl of 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT, Sigma M5655, USA) solution (2.5 mg / ml) was added and incubated for an additional 3 hours. Subsequently, discard the cell culture solution, and 200 µl of dimethyl sulfoxide (DMSO, Sigma D2650, USA) was treated with 200 µl of each well, followed by stirring. 100 µl of 96 wells was taken with Enzyme-Linked Immunosorbent Assay (ELISA). Absorbance was measured at 570 nm. The degree of cytotoxicity was expressed as a percentage based on the absorbance intensity of the control group using pure water. The results were as shown in Table 3 and FIG.

Cell growth effect (%) = (absorbance at extract treatment / absorbance of control) × 100

Proliferative effect (%) Control group 100 Comparative Example 1 99 Comparative Example 2 101.1 Comparative Example 3 102.1 Comparative Example 4 99.8 Comparative Example 5 101.4 Comparative Example 6 101.9 Comparative Example 7 100.6 Comparative Example 8 98.2 Comparative Example 9 102.4 Comparative Example 10 103.1 Comparative Example 11 103.2 Comparative Example 12 104.2 Comparative Example 13 106.1 Experimental Example 1 of the Invention 112.4

As shown in Figure 1, compared to the control when Comparative Examples 1 to 13 and Experimental Example 1 of the present invention when treated, the composition according to the present invention even after prolonged use does not induce scalp irritation due to cytotoxicity Could.

In addition, as shown in Figure 2, in the 0.1 to 10% treatment of the composition of Experimental Example 1 of the present invention, compared to the control, 10% treatment 112, 5% treatment 108, 1% treatment 105, 0.5 It was confirmed that there was no statistical difference with the untreated group when the control group was converted to 100% at 102% and 101% at 0.1% treatment. Therefore, it was found that the composition according to the present invention does not cause scalp stimulation due to cytotoxicity even after long-term use.

Formulation Example 1 Hair Tonic ingredient Weight ratio (%) denatured alcohol 50 menthol 0.02 glycerin 3 Salicylic acid 0.05 Iris root extract 0.5 Ginseng extract 0.5 칡 extract 0.5 Nettle extract 0.5 Sewao Extract 0.5 Ginseng extract 0.5 Sugar extract 0.5 Centella extract 0.5 Sancho Extract 0.5 Machi Prefecture Extract 0.5 Bokryeong Extract 0.5 Spices and Colors A reasonable amount Purified water Balance (to 100)

[Formulation Example 2] Hair Lotion ingredient Weight ratio (%) Cetostearyl alcohol 2.0 EDTA 2Na 0.2 Hydroxymethylcellulose 0.5 Mineral oil 5.0 Iris root extract 0.5 Ginseng extract 0.5 칡 extract 0.5 Nettle extract 0.5 Sewao Extract 0.5 Ginseng extract 0.5 Sugar extract 0.5 Centella extract 0.5 Sancho Extract 0.5 Machi Prefecture Extract 0.5 Bokryeong Extract 0.5 Glycerin, Beta Glucan A reasonable amount Preservatives, Spices and Colors A reasonable amount Purified water Balance (to 100)

[Formulation Example 3] Hair Shampoo ingredient Weight ratio (%) Sodium lauryl sulfate 36.0 Cocamidopropyl Betaine 8.0 Cocamide Diei 2.0 Palmidine Malate 2.0 Glycolstearate 1.5 Polyquaternium-10 0.5 Citrix Acid 0.1 glycerin 2.0 Dimethicone 0.2 Iris root extract 0.5 Ginseng extract 0.5 칡 extract 0.5 Nettle extract 0.5 Sewao Extract 0.5 Ginseng extract 0.5 Sugar extract 0.5 Centella extract 0.5 Sancho Extract 0.5 Machi Prefecture Extract 0.5 Bokryeong Extract 0.5 glycerin A reasonable amount Beta Glucan A reasonable amount Preservatives, Spices and Colors A reasonable amount Purified water Balance (to 100)

Formulation Example 4 Hair Rinse and Treatment ingredient Weight ratio (%) Propylene glycol 2.0 Cetrimonium chloride 1.0 Cetyl alcohol 3.0 Stearyl alcohol 3.0 Mineral oil 0.5 Citrix Acid 0.2 Dimethicone 1.0 Iris root extract 0.5 Ginseng extract 0.5 칡 extract 0.5 Nettle extract 0.5 Sewao Extract 0.5 Ginseng extract 0.5 Sugar extract 0.5 Centella extract 0.5 Sancho Extract 0.5 Machi Prefecture Extract 0.5 Bokryeong Extract 0.5 glycerin A reasonable amount Beta Glucan A reasonable amount Preservatives, Spices and Colors A reasonable amount Purified water Balance (to 100)

Experimental Example  2: Animal test of hair growth functional evaluation of the composition of the present invention

A hair score test using an animal experiment was performed to verify the efficacy of the composition obtained in Example 1.

As an animal test model for evaluating hair growth induction promoting effect, the C57BL / 6 mouse model was used. Is present only in hair follicles, and the melanin synthesis matches well with the hair growth cycle, which has the advantage of determining the hair growth cycle by the color of the skin. It is used.

42-56 days old mice (C57BL / 6, male) were used. First, the hair on the back of the mouse was removed using an electric razor, and each mouse was weighed and divided into 10 pieces so that the weight was evenly distributed. A control group, 5% minoxidil, the composition of Comparative Example 1, and the composition of Experiment 1 according to the present invention were applied to the hair removal site from the next day with the adaptation period for one day, wherein 50% ethanol was used as the control group. 10% of the concentrated solid of the composition of Experimental Example 1 according to the invention was used diluted with 50% ethanol. 100 µl each was applied to the back of the mouse once per day for 15 days to observe the growth phase induction promoting action.

As shown in FIG. 3, the control group, 5% minoxidil group, Comparative Example 1, Comparative Example 12, Comparative Example 13, and Experimental Example 1 according to the present invention showed little difference on day 3, and after 7 days had elapsed. Control group, 5% minoxidil group, Comparative Example 1, Comparative Example 12, Comparative Example 13 and Experimental Example 1 according to the present invention showed a slight difference, after 12 days, the control group, 5% minoxidil group, Comparative Example 1, comparison Example 12, Comparative Example 13 and Experimental Example 1 according to the present invention began to show a significant difference, Experimental Example 1 according to the present invention was excellent in the hair growth promoting effect than the control group was recovered almost completely the hair loss.

Experimental Example  3: hair growth functional evaluation test of the composition of the present invention

Small scale clinical trials were conducted by selecting female and male alopecia patients through the basic formulations that can be commercialized with the herbal compositions of the present invention, whose efficacy was primarily confirmed in animal experiments. Subjects were divided into two groups of 20 adult men and women with hair loss ranging from 30 to 60 years old (15 males, 5 females). 5% of minoxidil sold at the pharmacy 5 ml once a day Product (Hyundai Pharmaceutical Co., Ltd.), Comparative Example 1, Comparative Example 12, Comparative Example 13, and Experimental Example 1 composition obtained according to Example 1 (The concentrated solids of the composition according to Example 1 were used by diluting with 35% ethanol. ) Was applied to the bald area for 3 months with a liquid tonic prepared by) to observe the results.

The evaluation of the effect was performed by five stages of evaluation of daily hair loss, visual judgment, and improvement of subjective symptoms during trituration, and the overall improvement was taken as the average of the evaluation scales. According to one point unit, as shown in Table 8.

Score Scale Used in Small Clinical Trials score Visual judgment Subjective symptoms Hair loss at tricycle One worse worse increase 2 immutability immutability immutability 3 Little improvement Little improvement Little decrease 4 Medium improvement Medium improvement Medium reduction 5 Much improvement Much improvement Much reduced

Small clinical trial results Experiment group judgment Minoxidil
5% group Comparative example
One Comparative example
12 Comparative example
13 Experimental Example 1 of the Invention Visual judgment 2.8 2.7 2.2 2.8 4.5 (90% improvement) Subjective symptoms 2.2 1.9 2.1 1.9 3.5 (45% improvement) Hair loss at tricycle 2.3 2.4 2.2 2.4 4.0 (75% improvement) Overall improvement (average) 2.43 2.2 2.2 2.3 4.16 (70% improvement)

As can be seen from Table 9, as a result of small scale clinical experiments, compared to the minoxidil treatment group, Comparative Example 1, Comparative Example 12 and Comparative Example 13, the Experimental Example 1 composition treatment group of the present invention, visual judgment, subjective symptoms, hair loss during trituration 45 to 90% of hair loss was improved. Comprehensive improvement was found to have an average 70% improvement.

Claims (4)

A composition for preventing hair loss and promoting hair growth comprising a medicinal herb extract, ginseng extract, ginseng extract, nettle extract, sewage extract, red ginseng extract, medicinal extract, centella extract, sancho extract, machi extract, and bokyeong extract.
According to claim 1, The iris root extract, ginseng extract, 칡 extract, nettle extract, sewage extract, ginseng extract, medicinal extract, centella extract, sancho extract, machihyeon extract and Bokryeong extract are 0.5 to 5 parts by weight, 0.5 ~ each 5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight, 0.5-5 parts by weight Part and 0.5 to 5 parts by weight of the composition for preventing hair loss and promoting hair growth.
According to claim 1, wherein the composition is shampoo, hair rinse, hair tonic, hair gel, hair lotion, hair pack, hair spray, hair mousse, hair treatment and hair dye, characterized in that any one selected from the group consisting of Hair loss prevention and hair growth promoting composition.
The composition for preventing hair loss and promoting hair growth according to claim 1, wherein the herbal extract mixture is included in an amount of 0.1 to 10 parts by weight based on 100 parts by weight of the total composition.

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