KR101440046B1 - Oriental composition for preventing loss of hair and promoting growth of hair, and method of preparing the same - Google Patents

Abstract

Herbal extracts, including mushroom extract, golden extract, mulberry extract, corn oil extract, mulberry extract, oolong extract, oolong extract, green tea leaf extract and ginseng extract; And a polyphenol including genistein and resveratrol, and a method for producing the herbal composition for hair loss prevention and hair growth promotion.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for preventing hair loss and promoting hair growth, and a method for producing the composition. [0002]

The present invention relates to a herbal composition for preventing hair loss and promoting hair growth, and a method for producing the composition.

Hair protects the scalp from the sun's rays and is an important measure of health when hair gets worse or worse. The human head usually has 100,000 Virgin Mary and they repeat the generation and disappearance through periodic changes of anagen, catagen, telogen and exogen.

Hair loss refers to a state in which the number of hair is smaller than the number of new hair. The cause of hair loss varies from person to person. However, degeneration of scalp cells due to aging, including genetic factors and hormone effects, Lack of oxygen supply due to excessive keratin / sebum discharge of scalp cells, clogging of pores, scalp inflammation due to stress and disease, and the like.

Hair loss is largely divided into anti - inflammatory and non - antagonistic. Anti-alopecia areata is caused by destruction of the hair follicles and the formation of fibrous tissue, which is a permanent hair loss condition, which is caused by trauma such as burns and wounds, radiation dermatitis, infection, folliculitis, papillary squamous cell carcinoma, lupus erythematosus, scleroderma, . On the other hand, as for the non-aggre- gated alopecia, there are alopecia such as alopecia areata, male alopecia, growth hair alopecia, hairy wall and the like, and non-invasive alopecia can be converted into anti- Anti - hypertrophic hair loss is not treated medically.

Among the non-contractile alopecia areata, more than 90% of the alopecia areata is caused by stress, and one or two circular or elliptical alopecia occurs, which occurs not only in the scalp but also in the eyebrows, eyelashes and whiskers. In case of severe alopecia, It also progresses to systemic alopecia.

Male alopecia are alopecia due to male hormone, and it is observed that the scalp of patients with this symptom shows a lot of oil because it is oily. This is because the sebaceous glands are developed by the hormone and the sebaceous glands are secreted, resulting in seborrheic dermatitis. Promote. Male hormones (androgens) are synthesized and secreted in the adrenal cortex and gonads, and the most powerful and representative testosterone is testosterone.

Testosterone is converted to the active male hormone dihydrotestosterone by an enzyme called 5α-reductase. 5 alpha-reductase (5a-reductase) is present in the hair follicle, and dihydrotestosterone shortens the hair follicle growth and reduces the size of hair follicles. In other words, the thick black hair does not grow enough, tapered and weakened, so that it falls out faster than normal, shorten the hair cycle, so the hair is weak and thin at first, the hair gradually falls off and is not new.

Thus, male pattern baldness is a genetic disorder in which various genes are expressed by interacting with certain environmental factors.

Currently, the drug is known to be used worldwide. Minoxidil and the recently approved US Food and Drug Administration's (FDA) approved 'Finasteride' can not completely cure baldness because of its limited effect. In addition to male hormones, growth factors such as cytokines, epidermal growth factor (EGF), platelet-derived growth factor (PDGF), transforming growth factor-alpha (TGF-alpha) and TGF-beta also affect hair growth.

In the histopathologic examination of patients with hair loss, inflammation is observed in about 50%, which stimulates cytokines and growth factors. EGF and TGF-α promote the formation of hair-like structures and TGF-β inhibits hair growth. In addition to genetic factors and physiological factors of hair loss caused by hormones, the cause of hair damage can be divided into physical cause and chemical cause. Physical causes are caused by technical stimuli (brushing or reverse hair), heat, etc., and they mainly cause morphological changes. However, they cause chemical damages by giving chemical changes to hair components by wave or dye, , And the melanin pigment of the hair is oxidized by the ultraviolet ray and discolored.

In addition, damage to hair can also be caused by cuts, air pollution, excessive diet, and unbalanced diet. Modern society is exposed to various kinds of oxidative stress by various pollutants, physical action factors, excessive smoking and stress, which leads to alopecia.

Currently, various products for preventing hair loss and promoting hair growth are widely used and widely used. However, chemical preservatives such as parabens, which are controversial about harmfulness, are used in some of these products. The chemical preservatives attack the mitochondria of cells Which causes cell mutagenesis and promotes cell apoptosis, has been reported.

There are patent documents for prevention of hair loss and promotion of hair growth. For example, Korean Patent Publication No. 2004-0008916 discloses a hair soap composition containing oil extracted from bark, Korean Patent No. 0609210 relates to a shampoo composition containing an extract extracted with ethanol from a root of Angelica gigas and burdock root, Korean Patent Publication No. 2004-0105048 discloses a shampoo composition containing ginseng, mulberry leaf, joining, licorice, Korean Patent Laid-Open No. 2004-0030036 discloses a composition comprising an extract obtained by adding water to water and extracting by low-temperature low-temperature bathing. Korean Patent Publication No. 2004-0030036 discloses a composition comprising a mixture of Hashuo, Seokchangpo, , Adding distilled water to the mixture, and extracting the extract.

Korean Patent Application No. 1997-034216 relates to a technique for preparing extracts by aging for 16 days or more for 15 days or longer in ethyl alcohol and mineral water containing 16 kinds of herbal medicines including Sulfuric Acid and Korean Patent No. 0603122, A method for producing a herbal medicine composition which comprises extracting a herb medicine containing herbaceous moths and mesophyll with water, extracting a herbal medicine such as chrysanthemum and white paper with vegetable oil, and extracting ginger with a mixed solvent of ethyl alcohol and water; In addition, there are patent documents such as Korean Patent Publication Nos. 2005-0050321, Korean Patent Publication No. 2004-0030036, Korean Patent No. 0624544, Korean Patent Publication No. 2006-0121060, and the like.

However, they do not inhibit or neutralize testosterone, and they are accompanied by problems such as skin irritation and cytotoxicity such as erythema and itching.

An embodiment of the present invention is to provide a herbal composition for preventing hair loss and promoting hair growth, which does not cause microorganism transition upon storage for a long period of time, has no toxicity to keratinocyte and has excellent antioxidant ability.

Another embodiment of the present invention is to provide a method for producing the herbal composition for preventing hair loss and promoting hair growth.

One embodiment of the present invention is a herbal extract comprising a mushroom extract, a golden extract, a mulberry extract, a corn oil extract, a mulberry extract, a ginseng extract, a medicinal plant extract, a green tea leaf extract and a ginseng extract; And a polyphenol including genistein and resveratrol. The present invention also provides a herbal composition for preventing hair loss and promoting hair growth.

The herbal composition may contain 96 to 98% by weight of the herbal extract and 2 to 4% by weight of the polyphenol.

The herbal composition may contain the herbal extract and the polyphenol in a weight ratio of 25: 1 to 40: 1.

Wherein the herbal composition comprises 20 to 25% by weight of the mushroom extract, 20 to 25% by weight of the golden extract, 15 to 20% by weight of the mushroom extract, 5 to 10% by weight of the mushroom extract, A herbal extract comprising 5 to 10% by weight of the extract, 5 to 10% by weight of the extract of the ginseng extract, 5 to 10% by weight of the extract of Sukhwajyang, 5 to 10% by weight of the green tea leaf extract and 5 to 10% by weight of the ginseng extract. And 1 to 2% by weight of the genistein and 1 to 2% by weight of the resveratrol.

The herbal composition may further comprise at least one member selected from the group consisting of silk extract, Huangyan extract and Huaxia bark extract, wherein at least one member selected from the silk extract, 10% by weight.

According to another embodiment of the present invention, there is provided a method of extracting bone marrow cells, comprising the steps of: Extracting the second extract by adding the mushroom, corn syrup, 칡, gosam, 지 황,, green tea leaves and ginseng to a hot water distiller; And a step of adding polyphenol including genistein and resveratrol to a complex extract obtained by mixing the first extract and the second extract and stirring the mixture to prepare a herbal composition for hair loss prevention and hair growth promotion ≪ / RTI >

The method for preparing the herbal composition may further comprise a step of cooling the steam generated in the step of extracting the second extract to obtain a distillate.

The method for preparing the herbal composition may further include removing solid impurities from the first extract before mixing the first extract and the second extract.

The method of preparing the herbal composition may further include removing solid impurities from the second extract before mixing the first extract and the second extract.

The step of extracting the first extract may be carried out at 40 to 80 ° C.

The step of extracting the second extract may be heated at 80 to 120 ° C and heated for 20 to 24 hours.

The stirring step may be stirred at 25-28 < 0 > C.

The stirring may include mixing 96 to 98% by weight of the complex extract and 2 to 4% by weight of the polyphenol.

The gold content is 20 to 25% by weight, the sheathing weight is 15 to 20% by weight, the mushroom content is 20 to 25% by weight and the marine oil is 5 to 10% by weight based on the total amount of the raw materials to be added. Wherein the green tea leaves are 5 to 10 wt%, the ginger silver is 5 to 10 wt%, the green tea leaves are 5 to 10 wt%, the ginseng is 5 to 10 wt% The genistein may be added in an amount of 1 to 2% by weight, and the resveratrol may be added in an amount of 1 to 2% by weight.

The step of extracting the second extract may further include at least one selected from the group consisting of silk, chaff, and bark by adding to the total amount of the raw materials to be added, and at least one selected from the silk, 10% by weight.

Other details of the embodiments of the present invention are included in the following detailed description.

When the herbal composition for preventing hair loss and promoting hair growth is applied to the scalp, there is no skin irritation, and hair loss and hair growth are promoted as sebum and abnormal hyperkeratosis are reduced.

1 is a graph showing the degree of human keratinocyte cytotoxicity against the herbal composition of Example 1. Fig.
2 is a graph showing antioxidant activity of the herbal composition of Example 1. Fig.
Fig. 3 is a photograph showing the presence of bacteria (A) and fungus (B) immediately after preparation of the second extract prepared in Example 1 and bacteria (C) and fungus (D) after 30 days of storage.
4 is a graph showing changes in scalp keratin before and after the use of the herbal composition according to Example 1 of the present invention.
5A and 5B are photographs showing changes in scalp keratin before use of the herbal composition of Example 1 and after 2 weeks of use, respectively.
FIG. 6 is a graph showing changes in the degree of cleansing of scalp pores before and after the use of the herbal composition according to Example 1. FIG.
FIGS. 7A and 7B are photographs showing changes in scalp pore cleanliness before use of the herbal composition of Example 1 and after 2 weeks of use, respectively.
8 is a graph showing changes in scalp sebum amount before use of the herbal composition of Example 1 and 2 weeks after use.
FIG. 9 is a graph showing the results of promoting hair growth using the herbal composition of Example 1. FIG.
FIG. 10 is a photograph of a mother-child test of each of the clinical cases 5 to which the herbal composition of Example 1 is applied.
11 is a photograph showing the state of hair before and after 3 months of use of the herbal composition according to Example 1 for subjects with alopecia.

Hereinafter, embodiments of the present invention will be described in detail. However, it should be understood that the present invention is not limited thereto, and the present invention is only defined by the scope of the following claims.

According to one embodiment, the herbal composition for preventing hair loss and promoting hair growth may include herbal extract and polyphenol.

The herbal extracts may include extracts of mushroom, golden extract, mulberry extract, corn oil extract, mulberry extract, gossypium extract, mulberry leaf extract, green tea leaf extract and gingerol extract, wherein the polyphenol is genistein and resveratrol ).

The herbal composition may contain 96 to 98% by weight of the herbal extract and 2 to 4% by weight of the polyphenol. Specifically, the herbal composition may contain the herbal extract and the polyphenol in a weight ratio of 25: 1 to 40: 1. When the herbal composition is contained within the content ratio range, skin irritation can be reduced, sebum and hyperkeratosis can be reduced, thereby preventing hair loss and promoting hair growth.

The mushroom, which is the component of the mushroom extract, is also called woody mud mushroom, and the name of mushroom mushroom is recorded in the name of mushroom. It is rich in calcium, potassium, magnesium, vitamin B2, vitamin C, fiber and amino acids, and contains a large amount of beta-glucan polysaccharide. The beta-glucan exhibits an immunomodulatory function and an immune function-enhancing effect that improve immunity when the immunity is decreased and suppress the immunity when the immunity is excessive. The above-mentioned mushroom is known to be a valuable medicinal substance which acts only on cancer cells and exhibits an anticancer effect of about 96.7%.

The mushroom extract may be contained in an amount of 20 to 25% by weight, specifically 22 to 23% by weight based on the total amount of the herbal composition. When the above mushroom extract is contained within the above range, the microorganism does not migrate when stored for a long period of time, has no toxicity to keratinocytes and has excellent antioxidant ability.

The golden extract is a natural ingredient extracted from gold, a perennial herb of lepidoptera. It inhibits the activity of tyrosinase, a melanin pigment-forming enzyme, blocks ultraviolet rays, and whitening effect. In addition, It is a herb medicine which is used for lowering the heat of color and controlling body temperature. Its range of use is wide.

The active ingredients of the gold extract include baicalin, baicalein, wogonin, wogonoside, and dimethyltetrahydroxyflavone (DTF), which are antibacterial, antiinflammatory, antioxidant, anti-atopic Material. In other words, the above-mentioned golden extract plays a role of maintaining the antifungal property of the product as a natural antimicrobial agent for plants while suppressing scalp itching and dandruff.

The gold extract may be contained in an amount of 20 to 25% by weight, specifically 23 to 25% by weight, based on the total weight of the herbal composition. When the gold extract is contained within the above range, the microorganism does not migrate when stored for a long period of time, has no toxicity to keratinocytes, and has excellent antioxidant ability.

The herringbone, which is a component of the herring root extract, is dried in the root bark of the peony. In the "Dongbibogam", the quality is slightly lower, and the taste is very high. It is very poisonous and it does not poison. It makes the woman's menstrual impurities, low back pain, abortion, It is said that it treats all blood diseases (sickness) and cavalry (气 disease). The dermis promotes circulation of blood, thereby eliminating the eosinophilia and painful back pain or arthralgia. It also has blood pressure strengthening action, antimicrobial action, improves blood circulation function, prevents hair hair loss, promotes hair growth, maintains black and shiny hair, and acts as a vegetable natural antimicrobial agent to maintain the buoyancy of the product.

The herringbone extract may be contained in an amount of 15 to 20% by weight, and more specifically, 17 to 18% by weight based on the total weight of the herbal composition. When the herringbone extract is contained within the above range, no microorganism transition occurs during storage for a long period of time, and there is no toxicity to keratinocyte and excellent antioxidant ability.

As a component of the above-mentioned extract of corn oil, the corn oil is a deciduous tree of the deciduous tree, and is a fruit of the corn oil. Corn oil contains cornucin, moronide, roganin, tannin, saponin and other glycosides and organic acids such as wine acid, malic acid and tartaric acid. It also contains vitamin A and a large amount of sugar. The above-mentioned corn oil has the effect of seeing the body, which is used in the internal medicine, gynecology and pediatric illnesses. It helps energy, lightens the body, has excellent antimicrobial activity and antioxidant ability, and is especially effective for preventing hair from whitening.

The extract of corn oil may be contained in an amount of 5 to 10% by weight, specifically 6 to 6.5% by weight based on the total weight of the herbal composition. When the above extract is contained within the above range, the microorganism does not migrate when stored for a long period of time, has no toxicity to keratinocytes and has excellent antioxidant ability.

It is a perennial plant, which is a component of the 칡 extract, and most of its stem survives without freezing in winter. The stem is classified as a tree because it grows thicker every year and forms a thick stem. It has been used for a long time as a horticultural crop and has been used as a health food such as nourishing tonic. In the oriental medicine, the root is used as a medicine called 葛根. It is used as sweating, fever and sedative. It releases the muscles in the cold, body aches and headache, opens the skin to sweat, releases the poison, I was cold and said I was going to heat. Containing a large amount of flavonoid component, it is excellent in skin cleansing and prevention of aging. Suppression of tyrosinase activity inhibits the production of melanin pigment, thereby excelling in skin whitening. The extract is effective for wound scarring and hemostasis. It prevents the skin from being damaged by harmful oxygen and loosens the eosinophilus to soften the skin.

The extract may be contained in an amount of 5 to 10% by weight, and more preferably 9 to 9.5% by weight based on the total weight of the herbal composition. When the extract is contained within the above range, the microorganism does not migrate when stored for a long period of time, has no toxicity to keratinocytes and has excellent antioxidant ability.

Gosam, a component of the Gosam Extract, is a perennial herb that is about 1m in height with a soybean. Its leaf shape is similar to that of a picture tree, so it has a nickname of massive, massive, and fatigue. It is said to have the same effect as ginseng, though it uses its taste to dry it. It is used for placebo, diuretic, antipyretic, analgesic, insecticide, jaundice, dysuria, omega, eczema, and dry circulation. It also has astringent hemostasis, and it can also be used for rituals and diarrhea, hemorrhoids, uterine bleeding and white dew. In the private sector, it has been widely used as a skin insecticide since it has been pulverized or dyed with roots, stem and leaves.

The ginseng extract may be contained in an amount of 5 to 10% by weight, and more specifically 9 to 9.5% by weight based on the total weight of the herbal composition. When the extract is contained within the above range, the microorganism does not migrate when stored for a long period of time, has no toxicity to keratinocytes and has excellent antioxidant ability.

As a component of the Sukjipu Extract, Sukjipphi is a herb medicine which is dried and dried nine times and then dried nine times. And it has a bitter taste and a warm nature with a taste but it is complementary to the blood and supplement (jin: a substance that is the basic material for life and activity) It has the effect of treating dizziness and darkening the head.

The above-mentioned Sukjipu is the main medicinal material of Sangmatsu, and it is used for symptoms such as internal heat, sore throat, and thirst which are manifested by weakness of various chronic diseases. It is widely used for excessive bleeding, weakness and dizziness due to women's birth or menstruation. It has been effective in treating Huhdam (虛 痰) for a long time and drank for a long time by tea. It was used with cough and asthma in combination with gyeongryeong (茯 笭) and half-summer (half summer). In addition, boiled pork with broth is effective when taking habitual constipation. It has a lot of nutrients and it is greasy. If you take it for a long time, it causes digestive disorder and causes side effects such as diarrhea and repetition.

The Sulfuric Acid extract may be contained in an amount of 5 to 10% by weight, and more specifically 5 to 5.5% by weight based on the total weight of the herbal composition. When the Sulfuric Acid extract is contained within the above range, the microorganism does not migrate when stored for a long period of time, has no toxicity to keratinocytes and has excellent antioxidant ability.

The typical antioxidant component of the green tea leaf extract is catechin, which is a type of polyphenol compound having a specific bitter taste and has a basic structure of flavan-3-ol.

The proper amount of active oxygen has a positive effect in the sterilization action of phagocytes related to the in vivo immune function. However, excessive amount of active oxygen which breaks the balance of oxidation-antioxidant causes protein denaturation, lipid oxidation in the living body, Causing oxidative stress. If there is no adequate amount of antioxidants in the body, the oxygen that is inhaled through the respiration creates various peroxides and peroxide radicals, which can damage normal cells. Therefore, it is important to consume antioxidants through vegetables and fruits. The catechins mainly include epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG) and optical isomers thereof. In general, these four types are called tea catechins, of which EGCG is the most abundant and the physiological activity is also known to be the highest. The monomeric catechin was found to be the most abundant in the body after ingesting the green tea leaf extract and the bioavailability of EGCG was the highest.

The green tea leaf extract may be contained in an amount of 5 to 10% by weight based on the total amount of the herbal composition, and may be contained in an amount of 6 to 6.5% by weight. When the green tea leaf extract is contained within the above range, the microorganism does not migrate when stored for a long period of time, has no toxicity to keratinocytes and has excellent antioxidant ability.

The gingkohwa which is a component of the gingkohwa extract is a bud of an indigo vine, a small funnel-shaped bud with a length of 15 to 35 mm, a light brown hairs on a magnifying glass, The split pieces have hairs and are about 2 mm in length. There are components of the flavonoids luteolin, lonicerin and ochnaflavone, and the iridoid system loganin, secologanin, It has been reported that n-dimethylacetal, sweroside, secologanoside 7-methyl ester, loniceracetalide A and B, phenylalaninocecoloate, Phenylalaninosecologanin, and the like.

The polyphenols contain phenolic compounds such as chlorogenic aicd, isochlorogenic acid, and inositol. The gingkowala extract was improved in symptoms by administering chlorogenic acid to laboratory animals infected with influenza virus as well as bacteria such as Salmonella typhi, S. paratyphi, and E. coli. In addition, it suppresses the edema of the feet and the like, reduces the degradation of IκBα in the liver, and suppresses the activity of NFκB, thereby exhibiting anti-inflammatory action. Further, the orc or flavone inhibits the activity of phospholipase A2.

The ginger extract may be contained in an amount of 5 to 10% by weight, and more preferably 6 to 6.5% by weight based on the total weight of the herbal composition. When the Ganoderma lucidum extract is contained within the above range, the microorganism does not migrate when stored for a long period of time, has no toxicity to keratinocytes and has excellent antioxidant ability.

The herbal composition may further include at least one herbal extract selected from the group of herbal extract ingredients, silk extract, Huanglian extract, and herbal extract.

The silk extract is a kind of protein extracted from the cocoon extracted from cocoon and is also called silk protein. It is composed of 75% of fibroin protein, 25% of sericin protein, and 3% of inorganic and carbohydrate. It is the highest purity protein among many proteins. Serine, glycine, aspartic acid, threonine and alanine are the constituent amino acids of sericin protein, which are 18%, 13%, 12%, and 30%, respectively, of the protein of fibroin and sericin protein, 8% and 6%, and it is composed of many other amino acids. It is also closely related to the amino acid composition of the natural moisture factor present in the human epidermis, suggesting the possibility of developing a skin-friendly material using sericin protein. In addition to sericin protein, fibroin protein Is characterized by the presence of 18 amino acids in most cases, and glycine and alanine contents are very high, unlike the keratin protein of wool fibers. It is very similar to the amino acid of collagen protein, which is the main protein that constitutes human skin. Glycine and alanine in fibroin protein are the most abundant amino acids of skin collagen protein. As described above, the fibroin protein having an amino acid structure similar to human skin is expected to have effects such as prevention of skin aging, moisturization, induction of skin collagen production caused by moisture loss of skin, and silk peptide having low molecular weight by hydrolyzing silk fibroin, It is also used as a material for the hair because it has an effect of covering and protecting the texture, and at the same time promoting the adsorption of the dye, thereby preventing the damaged part of the hair surface, that is, scale damage or discoloration.

The above-mentioned Huangliao extract is a root of Coptis Chinensis FRANCH, a perennial herbaceous plant belonging to the family Ranunculaceae. Berberine, Coptisine, Worenine, Palmatine and the like are known as the active ingredients. have. Taste is cold. The efficacy has a fire effect, and it shows an excellent therapeutic response to all kinds of heat-related diseases. Such as infectious fever, typhoid fever, and the effect is remarkable, the fever of the chest with a lot of fever, thirst, and resolves the worrying symptoms. The effect of burning or burning is due to the fact that the wound is easy to heal because it acts as a sterilizer as the heat is lowered. It treats the symptoms of heat accumulation in the body and the elevation of blood pressure. In addition, if you wash it with diluted water such as conjunctivitis, you will get the effect of sterilization and heat dissipation. Taking a small amount, it has a high effect of promoting digestion, inflammation in the stomach, so that the chest is aching, the stomach is hungry and the head is sick, and it is also effective in relieving the symptoms of heartburn caused by nervous irritation. There is also a high rate of cure for stomatitis, tongue inflammation, and corneal inflammation due to heat. It is effective for symptomatic fever and sore throat as well as preventing diarrhea by suppressing diarrhea, and stopping pulmonary tuberculosis, hemorrhage, nosebleed and fecal bleeding. The sterilizing action is remarkable and is widely used for skin infections. In animal experiments, antimicrobial, anti-inflammatory, antipyretic, stimulating bile secretion, and blood pressure lowering appeared.

The extract of Mulberry bark extract is a substance extracted from the white layer of the root of the root of the mulberry tree and the brown cork layer of the mulberry tree. The mulberry bark is a medicinal herb that is used for removing the inflammation of the lung and stopping the cough. Chest pain, coughing, and bloating of chest pain due to leachate from leukocyte pleural effusion are effective at the onset of chronic bronchitis or after pneumonia healing. It also has a high diuretic effect and is used early in acute gastritis. In each case, rheumatism, etc., the effect is further enhanced by using Peppermint, Peppermint, and Jingu. In the early stage of epidemic hepatitis, a small amount of sugar may be added to the two parts of the bark. Continue once a day for one week to help with coughing, diarrhea and depression. In addition, it has been proved that it acts as a therapeutic agent for coronary artery sclerosis hypertension and essential hypertension in the animal experiment. According to the ancient literature, there is an example of repairing the bamboo leaf by making the bamboo leaf as a thread and binding the knife which was wounded by the knife. It is said that it is not necessary to pull the thread when it is absorbed by the living body when the wound is sutured with an alfalfa sprout thread. This indicates the effect of external application. It is expected to be applied not only to cosmetic raw materials but also to external medicine.

The additional herbal extract may be contained in an amount of 5 to 10% by weight based on the total weight of the herbal composition, and may be 8 to 10% by weight. When contained within the above range, the microorganism does not migrate when stored for a long period of time, has no toxicity to keratinocyte, and has excellent antioxidant ability.

The genistein, a type of polyphenol, was polyphenol isolated from polyphenol soy isoflavone. In one embodiment, 99% of genistein having a purity of 99% was used. The genistein is a feedstock for MMP, a US multinational cosmetics and health food supplier.

The genistein is an estrogen analogue of the plant and promotes the replication of normal cells together with a strong antioxidative, anti-inflammatory and anti-cancer effects. Clinical trials of SCI have been published as a component to suppress hair loss. Clinical results have also been reported recently as substances suppressing photoaging. In one embodiment, it has a hormone effect as an estrogen analogue of genistein, a cell regeneration effect, an effect of suppressing photoaging, suppression of hair loss, and promotion of hair growth.

The genistein may be contained in an amount of 1 to 2% by weight based on the total amount of the herbal composition, and may be included in an amount of 1.5 to 1.6% by weight. When the genistein is contained within the above-mentioned range, no microorganism transition occurs during storage for a long period of time, there is no toxicity to keratinocyte, and the antioxidant ability is excellent.

The resveratrol is a polyphenol extracted from plants such as grapes, peanuts and peanuts. In one embodiment, 99% resveratrol having a purity of 99% is used, and is a feedstock for US MMP. David Sinclair, a professor at Harvard Medical School, in August 2003, who has been proven as "chemopreventive activity" by Jang et al. In 1997 and published in Science magazine (vol 275, pp 218-220) Since the announcement of the extension effect in Nature, research and development has become one of the most active physiologically active substances worldwide.

The resveratrol has anti-cancer, anti-cancer, anti-thrombotic, anti-inflammatory, anti-hyperlipidemic, antibacterial and liver protecting action, anti-obesity and anti-metabolic syndrome action, In one embodiment, the hormone effect as an estrogen analog of resveratrol, the effect of prolonging the life of the cell, the suppression of hair loss, and the action of promoting hair growth are performed.

The resveratrol may be contained in an amount of 1 to 2% by weight based on the total amount of the herbal composition, and more specifically, 1.5 to 1.6% by weight. When the resveratrol is contained within the above range, the microorganism does not migrate when stored for a long period of time, has no toxicity to keratinocytes and has excellent antioxidant ability.

The herbal composition may be prepared by the following method.

First, the above-mentioned extracts are extracted by injecting the above-mentioned gold and mulberry into an ultrasonic extractor. In addition, the mushroom, corn syrup, 칡, gosam, Sukjihwang, green tea leaves and ginseng are added to the hot water distiller to extract the second extract. At this time, the order of extracting the first extract and extracting the second extract may be reversed.

The extraction of the first extract may be performed using the ultrasonic extractor at a temperature of 40 to 80 ° C, specifically 40 to 50 ° C, more specifically 40 to 41 ° C. The loss of active ingredients weak to heat can be minimized when performed at temperatures within the above range.

The extraction of the second extract may be performed by heating at a temperature of 80 to 120 ° C., specifically, at a temperature of 100 to 110 ° C. . Also, the heating may be performed for 20 to 24 hours, and specifically for 23 to 24 hours. When it is conducted at a temperature within the above-mentioned range and within the above-mentioned range, it is heated to 120 ° C, which is the melting point of heat-resistant polyphenols (isoflavones), thereby minimizing the loss of heat-resistant polyphenols. Sufficient steam can be generated while pulverizing the component that interferes with hot water.

At the time of extracting the second extract, at least one selected from the group consisting of silk, yellow radish and coriander can be further added to the hot-water still.

After extracting the second extract, the vapor generated during the extraction of the second extract may be cooled to obtain a distillate.

Next, the polyphenol containing genistein and resveratrol may be added to a complex extract obtained by mixing the first extract and the second extract, followed by stirring to prepare the herbal composition.

At this time, when the first extract and the second extract are mixed, they may be mixed using the first extract obtained by removing solid impurities from the first extract and the second extract obtained by removing solid impurities from the first extract.

The combined extract and the polyphenol may be mixed with each other at 1 to 2% by weight and 1 to 2% by weight, specifically 1.5 to 1.6% by weight and 1.5 to 1.6% by weight, respectively, When mixed in a ratio range, it is possible to reduce skin irritation, reduce sebum and abnormal hyperkeratosis, thereby preventing hair loss and promoting hair growth.

The stirring may be carried out at 25 to 30 ° C, specifically at 25 to 28 ° C, more specifically at 25 to 27 ° C. If stirring is performed within the temperature range, it is possible to prevent the destruction of polyphenols which are weak in heat.

The content of the ingredients of each ingredient to be added in the preparation of the herbal composition is the same as the content of each ingredient constituting the herbal composition described above based on the total amount of the ingredients.

Hereinafter, specific embodiments of the present invention will be described. However, the embodiments described below are only intended to illustrate or explain the present invention, and thus the present invention should not be limited thereto.

(Manufacture of herbal composition)

Example  One

200 g of gold and 200 g of Mulberry were thoroughly washed with purified water and extracted with butylene glycol in an ultrasonic extractor at 40 ° C for about 5 hours to obtain a first extract. Then, the first extract was centrifuged at 10,000 to 12,000 rpm at 5 DEG C for 30 minutes in a centrifuge to remove solid impurities.

The herb medicinal materials of 200g of green mushroom, 50g of green tea leaf, 50g of ginseng, 50g of ginseng, 50g of ganghwha 50g, 100g of sorghum ginseng and 100g of ginseng were thoroughly washed in purified water, Lt; RTI ID = 0.0 > 100 C < / RTI > to obtain a second extract. At this time, in order to obtain an active ingredient volatilized by the steam during the process of obtaining the second extract, a steam recovery cold water cooling tube was installed to obtain a distillate. Then, the second extract was centrifuged in a centrifuge at 10,000 to 12,000 rpm at 5 캜 for 30 minutes to remove solid matter impurities.

15 g of genistein and 15 g of resveratrol were added to 970 g of the combined extract obtained by mixing the first extract and the second extract from which solid impurities were removed and the mixture was stirred at 3,000 rpm for 30 minutes Followed by stirring to prepare a herbal composition.

The scientific names of raw materials used in the preparation of the herbal composition are summarized in Table 1 below.

Raw material Scientific name Gold Scutellaria baicalensis Georgi root Mural Paeonia suffruticosa root Situation mushroom Phellinus linteus Green tea leaves Camellia Sinensis Leaf Extract Corn oil Cornus officinalis fruit GoSam Sophora angustifola root Gold silver Lonicer aflower Sookmyung Rehmannia glutinosa var.purpurea MAKINO 칡 Pueraria thunbergiana Benth root Genistein Genistein Resveratrol Resveratrol

Test Example  1: Cytotoxicity test of herbal composition

In order to confirm the degree of scalp stimulation during long-term use of the herbal composition, cytotoxicity to human keratinocytes was evaluated.

The evaluation method is the same as in Example 1 except that 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (3- (4,5- yl) -2,5-diphenyl tetrazolium bromide (MTT).

The MTT assay is a method for measuring the degree of cytotoxicity of a test substance by forming a chromogenic product called 'atazan' using the dehydrogenase action of mitochondria which is active only in living cells.

The cultured human keratinocytes were counted in a 24-well plate at 5 × 10 3 cells / well using a heamacytometer and then injected in a divided manner. When the culture was cultured in DMEM (dulbeco's modified eagle's medium) containing 10% FBS (fetal bovine serum) for 48 hours and cultured by 25% to 30% of the culture vessel surface area, the herbal composition prepared in Example 1 contained 0.1 to 10% And the cells were cultured for 24 hours. After cultivation, 50 μl of MTT (Sigma M5655, USA) solution (2.5 mg / ml) was added and further cultured for 3 hours. Then, 200 μl of dimethyl sulfoxide (Sigma D2650, USA) was treated with 200 μl each of the wells, and 100 μl of each was taken in 96 wells and eluted with ELISA (Enzyme-Linked Immunosorbent Assay) at 570 nm The absorbance was measured. The degree of toxicity of the cells was expressed as a percentage based on the absorbance intensity of the control group (untreated group) using pure water, and the results are shown in Fig.

FIG. 1 is a graph showing the degree of human keratinocyte cytotoxicity against the herbal composition of Example 1, and is a graph showing the cytotoxicity test result of the herbal composition according to the present invention on human keratinocytes. FIG.

1, when the herbal composition was contained at concentrations of 10%, 5%, 1%, 0.5% and 0.1%, there was no significant difference from the control group due to the difference in absorbance intensity from 3.55 to 9.73% . Accordingly, it can be seen that the herbal composition according to one embodiment has antioxidant ability, so that the scalp stimulation due to cytotoxicity does not occur even when it is used for a long period of time.

Test Example  2: of the herbal composition Antioxidant ability  evaluation

The antioxidant activity of the herbal composition of Example 1 was measured by using 1,1-diphenyl-2-picryl hydrazyl (hereinafter referred to as DPPH) (Sigma D9132-1G, USA) .

The DPPH generates free radicals in ethanol. Thus, antioxidant activity was measured by confirming to some extent the amount of free radicals produced by mixing the herbal composition of Example 1 with DPPH at a certain ratio.

Specifically, 0.5 ml of a 0.1 mM DPPH solution prepared from ethanol and 0.4 ml of a complex extract were added to 0.4 ml of ethanol so that the final concentration was 0.1% to 10%. Strongly vortexing for 10 seconds and storage in a dark place for 30 minutes. Absorbance was measured at 517 nm using an enzyme-linked immunosorbent assay (ELISA). The degree of antioxidant activity was expressed as a percentage based on the absorbance of the control using pure water, and the results are shown in FIG.

Chemical contaminants from environmental pollutants and excessive hair products are the main cause of excessive oxidative stress in hair. Such excessive oxidative stress can convert the growing hair into a dormant period, and it can worsen various hair conditions and cause hair loss. It is also known that oxidative stress may cause alopecia areata due to the mechanism of activating the type 2 beta - corticotropin hormone receptor around the hair follicles. The herbal composition according to one embodiment has an antioxidant ability, and it is confirmed that DPPH has free radical scavenging ability to prevent hair growth and alopecia.

FIG. 2 is a graph showing the antioxidative activity of the herbal composition of Example 1, and more specifically, a graph showing antioxidative activity of the herbal composition according to the present invention through DPPH free radical scavenging ability.

2, when the herbal composition was contained at concentrations of 10%, 5%, 1% and 0.5%, respectively, as compared with the control group using purified water, DPPH free radicals in the reaction system were 94.21%, 85.23%, 69.89% % And 52.13%, respectively. These results indicate that when the herbal composition is applied to hair-related products, the herbal composition has low cytotoxicity and antioxidative ability even at a high concentration of 1 to 10%, thereby preventing hair loss and promoting hair growth.

Test Example  3: Bacteriostatic ability  evaluation

It was confirmed that the herbal composition of Example 1 had spontaneous bactericidal ability capable of inhibiting the microbial transition without the preservative treatment.

In order to confirm the number of viable bacteria in the herbal composition, the number of live bacteria was measured immediately after the second extract prepared in Example 1 was prepared and after being left at room temperature for 30 days.

Specifically, 0.1 ml of the second extract solution prepared in Example 1 was plated on a retinol agar medium (Difco, USA) and cultured at 30 ° C for 48 hours to confirm the presence of bacteria. The cells were plated on a Rose agar medium (Difco, USA) and cultured at 25 ° C for 72 hours to confirm the presence of fungi. The results are shown in FIG.

Fig. 3 is a photograph showing the presence of bacteria (A) and fungus (B) immediately after preparation of the second extract prepared in Example 1 and bacteria (C) and fungus (D) after 30 days of storage.

3, the presence of bacteria (A) and fungus (B) immediately after the preparation of the second extract was confirmed by a method of post-smearing the culture on an agar medium. As a result, it can be confirmed that no live cells existed. In addition, it can be confirmed that bacteria (C) and fungus (D) did not exist even after being left at room temperature for 30 days. Accordingly, when the herbal composition according to one embodiment is stored at room temperature for a long period of time, it has antibacterial ability to inhibit microorganism transition without addition of preservatives, thereby preventing abnormal transitions of dandruff-producing bacteria and the like, thereby preventing hair loss and promoting hair growth Able to know.

Test Example  4: Changes in scalp mass using herbal composition, pore clearance, and Amount of fat  Clinical trials of change

The changes in the mass of the scalp, the pore state, and the degree of change in sebum volume using the herbal composition of Example 1 were investigated through an external institution (Dermar Pro / Dermatological Research Institute, Korea) administered by the Korea Food and Drug Administration (KFDA) for Good Clinical Practice.

Fifteen female subjects aged 20 to 50 years (mean age 39.1 ± 6.3 years) with osteogenic scalpels suitable for the purpose of the test were selected and the photographs of the keratin and pore clearance of scalp before use and after 2 weeks of use of the herbal composition And the scalp condition was assessed using the paired t-test method of the SPSS Package Program 11.5 (p <0.05). Where p is the significance probability and p <0.05 indicates significance. The results were calculated according to the following formula (1), (2) and (3).

[Formula 1]

Increase / decrease ratio (%) = (measured value before use - measured value after use) / measured value before use X 100

The measuring instruments are shown in Table 2 below.

Measuring instrument Measurement site Cortical keratinization AramoTs ^® (x60)
(AramHuvis, Korea) scalp Scalp Pore Shooting AramoTs ^® (x200)
(AramHuvis, Korea) scalp Scalp sebum measurement Sebumeter ^®
(C + K, Germany) scalp

4-1: Clinical examination of mass change of scalp

AramoTS ^® (AramHuvis, Korea) Scalp Enlargement System (AramHuvis, Korea) was used to magnify the corneal scalp at each evaluation point, and the two researchers used 7 images The results are shown in Tables 3, 4, and 5A and 5B (Table 3). The results are shown in Tables 3, 4 and 5A Respectively.

Number of subjects (N) Arithmetic mean of measurements Standard Deviation Standard mean error Probability of significance
(p-value) Before use 15 0.00 0.00 0.00 - After 2 weeks of use 15 -2.10 0.54 0.14 0.000 *

4A and 4B are graphs showing changes in scalp keratin before and after the use of the herbal composition of Example 1. Figs. 4A and 4B are graphs showing changes in scalp keratin before and after the use of the herbal composition of Example 1, It is a photograph showing the change.

Referring to Table 3, FIG. 4, and FIGS. 5A and 5B, it can be seen that the curd keratin is decreased at the point of 2 weeks after the use of the herbal composition, and the condition of the scalp keratin is excellent.

4-2: Clinical examination of changes in scalp pore clearance

AramoTS ^® (AramHuvis, Korea) scalp enlargement system was used to enlarge the pores of the scalp at each evaluation point using a 200x lens, and then the two researchers used 7 images The results are shown in Tables 4, 6, and 7, respectively. The results are shown in Tables 4, 6, and 7 below. 7A and 7B.

time Number of subjects (N) Arithmetic mean of measurements Standard Deviation Standard mean error Probability of significance
(p-value) Before use 15 0.00 0.00 0.00 - After 2 weeks of use 15 -1.47 0.44 0.11 0.000 *

FIG. 6 is a graph showing the change in scalp pore clearance before and after the use of the herbal composition of Example 1. FIGS. 7A and 7B are graphs showing changes in scalp pore clearance before use and after 2 weeks of use of the herbal composition of Example 1, It is a photograph showing the change.

Referring to Tables 4, 6, and 7A and 7B, it can be confirmed that the scalp pores are cleaned at the point of 2 weeks after the use of the herbal composition.

4-3: scalp Amount of fat  Clinical trials of change

The scalp sebum level was measured three times using Sebumeter ^® (C + K, Germany) at each evaluation point, and the average value was analyzed. The results are shown in Table 5 and FIG.

Number of subjects (N) Arithmetic mean of measurements Standard Deviation Standard mean error Probability of significance
(p-value) Decrease (%) Before use 15 100.87 56.46 14.58 - - After 2 weeks of use 15 61.96 45.51 11.75 0.002 * 38.57 ▼

8 is a graph showing changes in scalp sebum amount before use of the herbal composition of Example 1 and 2 weeks after use.

Referring to FIG. 8, it can be seen that the amount of scalp sebum decreases at the point of 2 weeks after the use of the herbal composition.

Test Example  5: Assessment of hair growth promotion of herbal composition

To evaluate hair loss prevention and hair growth promotion of the herbal composition of Example 1, 10 male and female subjects aged 20 to 40 years (mean age 32.1 ± 5.3 years) with alopecia are selected and classified according to one embodiment The results are shown in Fig. 9. The results are shown in Fig. 9. The results are shown in Fig.

For the control group, pure water with no hair growth promoting effect was used in the same type of container. For the remaining 5 subjects, 50 ml of the herbal composition of Example 1 was applied daily to the scalp. The measurement was performed twice before and three months after use. The measurement was performed by a mother-blood test and a general visual examination (digital camera). The measurement equipments are shown in Table 6 below.

Measuring instrument Measurement site Measurement point Mother cycle test Multicam bomtec (x80)
(Damomedica, Korea) scalp 1) Before use
2) After 3 months of use Visual inspection Nikon DSLR D40
Standard zoom lens scalp 1) Before use
2) After 3 months of use

5-1: Mother period  inspection

The maternal periodic test is a phototricogram that examines the period of hair growth. The hair at the site to be measured was cut to a diameter of 5 mm, and then the position to be measured with a henna was displayed. Then, the lens was photographed at the same position with a Multicam bomtec 80 magnification lens and immediately after cutting and after 3 days, . It is possible to know whether the hair loss is progressing or progressing through the maternal periodic examination.

The maternal periodic rate, which is expressed as a percentage of hair growth, was measured through the maternal periodic test. The maternal periodic rate was measured according to the following formula 2. The maternal periodicity test was performed once before use, after 3 months after use, The circuit was measured twice in total. The results are shown in Table 7, FIG. 9, and FIG.

[Formula 2]

(%) = (Number of hairs growing after 3 days of cutting / number of cutting of initial hair) X 100

Number of subjects (N) Arithmetic mean of measurements Standard Deviation Standard mean error Probability of significance
(p-value) Decrease (%) Control group 5 -1.88 1.47 0.66 - - Subject 5 27.8 3.35 1.50 0.0000044 27.80 △

FIG. 9 is a graph showing the results of promoting hair growth using the herbal composition of Example 1, and FIG. 10 is a photograph of the mother periodicity test of each of the clinical cases 5 to which the herbal composition of Example 1 was applied.

Referring to Table 7, FIG. 9, and FIG. 10, alopecia of the control group continued, while subjects using the herbal composition according to one embodiment stopped alopecia, showed hair growth, and increased hair growth levels have.

5-2: Visual inspection

Visual inspection was carried out at the same time as the maternal periodic examination. In order to provide a basis for the visual inspection, pre-clinical photographs were taken with a Nikon DSLR D40 standard zoom lens, and the results are shown in FIG.

11 is a photograph showing the hair condition of a subject having alopecia before and after using the herbal composition of Example 1. Fig.

Referring to FIG. 11, in most of the control groups using water for 3 months, there was no change in alopecia or alopecia progressed. However, subjects using a herbal composition according to one embodiment showed a remarkable change in hair growth and alopecia Can be confirmed.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, And falls within the scope of the invention.

Claims (18)

1. A herbal composition for preventing hair loss and promoting hair growth, comprising:
20 to 25% by weight of a mushroom extract, 20 to 25% by weight of a golden extract, 15 to 20% by weight of a mulberry extract, 5 to 10% by weight of a mushroom extract, 5 to 10% 5 to 10% by weight of at least one extract selected from the group consisting of silk extract, Rhodiola extract and Mongolian bark extract, 5 to 10% by weight of water extract, 5 to 10% by weight of green tea leaf extract, 5 to 10% % Herbal extracts; And
A polyphenol comprising 1 to 2% by weight of genistein and 1 to 2% by weight of resveratrol, based on the total amount of the herbal composition, to promote hair growth and hair growth. delete delete delete delete delete Extracting the first extract at 40 to 80 占 폚 by adding the extract of gold and mulch into an ultrasonic extractor;
At least one selected from the group consisting of silk, rhododendron, and phytophthora, and at least one selected from the group consisting of mushroom, mushroom, mushroom, mushroom, ginseng, mushroom, green tea leaf and ginseng are added to a hot water distiller and heated at 80 to 120 ° C for 20 to 24 hours 2 extract; And
A step of adding polyphenol including genistein and resveratrol to a complex extract obtained by mixing the first extract and the second extract and stirring the mixture; and a step of preparing a herbal composition for promoting hair loss prevention and hair growth As a method:
Wherein the stirring step comprises: A composition comprising 20 to 25% by weight of a mushroom extract, 20 to 25% by weight of a golden extract, 15 to 20% by weight of a mulberry extract, 5 to 10% by weight of a mushroom extract, 5 to 10% And 5 to 10% by weight of at least one extract selected from the group consisting of silk extract, Huanghui extract and Huaxia bark extract, and 5 to 10% Preparing a complex extract by mixing the first extract and the second extract so as to include 1 to 2% by weight of genistein and 1 to 2% by weight of resveratrol relative to the total amount of the herbal composition; And then stirring the mixture at 25 to 30 DEG C to prepare a herbal composition for hair loss prevention and hair growth promotion. 8. The method of claim 7,
The method for preparing the herbal composition comprises:
Further comprising the step of cooling the steam generated in the step of extracting the second extract to obtain a distillate. 8. The method of claim 7,
The method for preparing the herbal composition comprises:
Further comprising the step of removing impurities of solid matter from the first extract before mixing the first extract and the second extract. 8. The method of claim 7,
The method for preparing the herbal composition comprises:
Further comprising the step of removing impurities of solid matter from the second extract before mixing the first extract and the second extract. delete delete delete delete delete delete delete delete

Images