KR100553810B1 - A composition containing Plantago asiatica Linne extract for skin external application - Google Patents

Abstract

The present invention is an external preparation for skin containing plantain outpost extract or plantain seed extract, which has the effect of improving skin wrinkles and improving wrinkles, improving elasticity, and having excellent moisturizing effect and no side effect.

Plantain extract, skin inflammation control, skin thickness, wrinkle improvement, skin elasticity, skin moisturizing

Description

A composition containing Plantago asiatica Linne extract for skin external application}

Figures 1a to 1c is a photograph showing the results of treatment of the external preparation composition of the skin in the change of epidermal thickness in hairless mice, Figure 1a is treated with a composition that does not contain the plantain outpost extract or plantain seed extract The control group is shown, and FIG. 1B shows an experimental group treated with a composition containing plantain seed extract, and FIG. 1C shows an experimental group treated with a composition containing plantain seed extract.

2a to 2c are photographs showing the collagen biosynthesis efficacy in the hairless mice of the composition according to the present invention, Figure 2a shows a control treated with a composition containing no plantain outpost extract or plantain seed extract, Figure 2b is plantain The experimental group treated with the composition containing the starch extract, Figure 2c shows an experimental group treated with the composition containing the plantain seed extract.

3 is a graph showing the wrinkle improvement effect of the composition according to the present invention.

The present invention relates to an external preparation composition for skin containing a plantain extract. More specifically, the present invention relates to an external preparation for skin containing an extract obtained by extracting from an appropriate solvent mixture from seeds and other homologous plants including the plant and the outer shell of the plantain, Plantaginaceae. The present invention can be prepared in the form of a flexible cosmetics, nourishing cosmetics, massage creams, nourishing creams, packs, gels, essences or skin adhesive type cosmetics, strengthening the thickness of the skin, to control the micro-inflammatory of the skin It also provides wrinkle improvement, elasticity improvement and excellent moisturizing effect.

As time passes, the skin of humans decreases the secretion of various hormones that regulate metabolism internally, and decreases the function of immune cells and the activity of cells, thereby reducing the biosynthesis of immune proteins and bioconstituent proteins necessary for the living body. Externally, due to the destruction of the ozone layer, the amount of ultraviolet rays reaching the earth's surface in the sun's rays increases and environmental pollution becomes more severe, resulting in a decrease in skin thickness, wrinkles, and elasticity. In addition, freckles and black mushrooms also increase, causing a number of changes. However, since modern society emphasizes a young and beautiful appearance, there is a great desire to prevent or solve the problem of skin thickness being reduced, wrinkles are increased, and elasticity is reduced by external factors such as internal factors and ultraviolet stress. Accordingly, various wrinkle improving agents, elasticity improving agents, whitening agents and the like have been developed.

In skin, symptoms such as wrinkles, loss of elasticity, dry skin, etc., change or decrease the content and arrangement of collagen, elastin, hyaluronic acid, proteoglycans, glycosaminoglycans, fibronectin and glycoproteins, which constitute the skin, The ability to combine with water is reduced, and as a result, the substrate material constituting the skin is reduced, and the skin's water content is lost. In addition, in most of the cells that make up the skin, tumor necrosis factor alpha (TNF α, Tumor necrosis factor α), a proinflammatory cytokine known to cause inflammation, and interleukin-1 beta (IL-1β, interleukin-) 1β) increases the biosynthesis of cyclooxygenase-2, an enzyme that produces prostaglandin, and matrix metalloprotease (MMP, an enzyme that degrades skin tissue by these inflammatory factors). It is known that the biosynthesis of matrix metalloproteinases is increased. Therefore, the type I collagenase (MMP-1) that degrades collagen, which is a substance that can thicken the skin by proliferating the skin cells or increasing the substance that constitutes the skin, and the substance that constitutes the skin Substances capable of inhibiting biosynthesis, substances capable of inhibiting biosynthesis of cyclooxygenase-2, substances capable of inhibiting biosynthesis of tumor necrosis factor, promoting the production of tropoelastin and fibrin in skin fibroblasts Developing a substance can alleviate symptoms of skin, such as wrinkles, loss of elasticity, and dry skin.

The present invention, by controlling the micro-inflammatory state of the skin, by controlling the thickness of the skin, to maintain the elastic and healthy skin condition, and also to increase the biosynthesis of collagen, elastin, glycosaminoglycans, etc. in the dermis to improve wrinkles and elasticity It aims at exhibiting a recovery effect. In addition, it aims to keep the skin moist to prevent wrinkles and to prevent and improve the drying of the skin.

In order to achieve the above object, the external composition for moisturizing the skin according to the present invention is characterized by containing a plantain extract.

In the above external composition for moisturizing the skin according to the present invention, the plantain extract is characterized in that the extract of Plantago asiatica .

In the external composition for moisturizing skin according to the present invention, the plantain extract is characterized in that the extract of plantain seed.

In the external preparation for moisturizing the skin according to the present invention, the plantain extract is characterized in that the extract of plantain outpost.

In the external composition for moisturizing skin according to the present invention, the plantain extract is characterized in that it is contained 0.001 to 10% by weight in the total composition.

In the external composition for moisturizing the skin according to the present invention, the plantain extract is characterized in that it is contained 0.01 to 5% by weight in the total composition.

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Hereinafter, the present invention will be described in more detail.

In the present invention, the plantain extract is an extract of plantain belonging to the same species as plantain, characterized in that the root of the plantain, seed, sheath, seed, starch or mixture thereof, and among them, it is preferable to use the seed or extract of plantain. .

Plantain used in the present invention is a perennial herb belonging to Plantaginaceae, young leaves are edible. In Chinese medicine, the roots are extracted and dried, called Chajeoncho, and the seeds are used as a medicinal herb, and are distributed in Korea, Japan, Sakhalin, Taiwan, China, Siberia, Eastern Himalayas, Java, Malaysia, etc. . The components of plantain outpost are glycosides such as acubin, plantaginin, and homoplantaginin, tannins, ursolic acid, phytosterol glycosides, and these sterols Beta-sitosterol palmitate (β-sitosterol palmitate), stigmasterol palmitate (stigmasterol palmitate) and the like are the main agents of the mucous swelling material. Planttaginis Semen contains mucilase (around 9%), planttasan, plantago mucilage A (L-arabinose, D-xylose, D-glucuronic acid, D). -galacturonic acid), iridoidal glycosides, acubin, genipiosidic acid, and flavone glycosides, plantagoside and other acetosides. (acetoside), syringin, choline and other fatty acids.

Plantain contains glycosides and tannins called "Plantaginine" and are used as diuretics, Jinhae, expectoration, health, branch, fever, anti-inflammatory, tonic, etc. It is also effective for seawater from waste heat. It also has antibacterial action, which is used to treat acute bacterial diarrhea, nephritis, cystitis and urethritis. For folk medicine, boil the fresh leaves on the boil to soften them and attach them to the toothache.

In the literature on plantain, the phenylpropanoid component of plantain seed is known to have diuretic and anti-inflammatory effects (especially hepatitis), and phytosterol glyicoside, which can contain water in the char and chaff, ), Mucilage (mucilage) is represented as a component of the food side is used for diet and constipation improvement. In addition, immune enhancing, antiviral, and plantagosides that activate complement have an immunosuppressive function as an alpha-mannosidase inhibitor. Plantain leaves also have a wound healing effect, and phenylethanoids reduce the swelling of mouse ears. Ursolic acid and fatty acids in plantain are also known to act as cyclooxygenase-2 (COX-2) inhibitors.

Looking at the patents for plantains and chajeon, "hair loss prevention and hair growth promoting composition (Korea Patent Registration 0382602)", "tea having a constipation symptom improving effect and its manufacturing method (Korea Patent 0344325)", "antibacterial plant extracts Toothpaste composition containing the (Korea Patent Registration 0313491) "," burn treatment composition (Korea Patent Registration 0167535) "and the like, and look at the patent on the skin," cosmetic composition for skin protection comprising a compound herbal extract (Korean Laid-Open Patent 2002-0044266) "," Herbal pack material for removing blemishes and freckles (Korean Laid-open Patent No. 2000-0049466) "," Acne preventive and therapeutic agent (Korean Laid-Open Patent 1999-0054326) "," Contains the tea extract Skin cleaner composition (Korean Patent Publication No. 1998-0067528) and the like are disclosed. About the ucubin (Aucubin) which is the main component of plantain, "pharmaceutical agent that inhibits the replication of hepatitis B virus (Korean Patent No. 0218052)" is registered. However, it has never been found to improve skin wrinkles, improve elasticity, or moisturize the skin by increasing skin thickness or controlling microinflammatory inflammation.

In the present invention, using the plantain extract can effectively improve the overall symptoms such as wrinkles, elasticity, moisturizing. The amount of plantain plantar extract or plantain seed extract is 0.001 to 10% by weight, preferably 0.01 to 5% by weight, based on the total weight of the composition, based on in vitro and in vivo experiments. If the extract content is less than 0.001% by weight, it is difficult to expect to act as an active ingredient, and if the extract content is more than 10% by weight, problems with formulation stability may occur. 0.01 to 5% by weight is more preferable in this respect.

The external preparation composition for skin according to the present invention is used for the purpose of improving wrinkles and elasticity, and is used for the purpose of moisturizing, and is not particularly limited in the formulation. For example, supple cosmetics, nourishing cosmetics, massage cream, nourishing cream, and pack It may be a cosmetic composition having a formulation of a gel or skin adhesive cosmetic, and may also be a transdermal dosage form such as a lotion, ointment, gel, cream, patch or spray.

The external preparation composition for skin according to the present invention may be a pharmaceutical composition, in which case the plantain extract may be used in medicine as it is or in the form of a pharmaceutically acceptable salt. The salt is not particularly limited as long as it is pharmaceutically acceptable. For example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, maleic acid, succinic acid, methanesulfonic acid , Benzene sulfonic acid, toluene sulfonic acid, naphthalene sulfonic acid and the like can be used. In addition to acid addition salts, base addition salts such as sodium hydroxide, potassium hydroxide, triethylamine, tert-butylamine can also be used.

The external preparation composition for skin according to the present invention, in the pharmaceutical formulation, the content of the plantain extract can vary widely depending on the formulation, according to conventional methods.

The external preparation for skin pharmaceutical composition according to the present invention may be formulated by adding various auxiliaries used in medicine, such as a carrier or other additives such as stabilizers, emollients, emulsifiers, etc. as necessary, so long as they do not adversely affect the active ingredient. .

In addition, the plantain extract containing composition according to the present invention can be administered parenterally, orally. As a parenteral route, transdermal administration is preferred, and topical application is most preferred. The formulation may be any formulation suitable for pharmaceutical preparations, including ointments, creams, injections, powders, granules, tablets and the like.

The preferred dosage of the pharmaceutical composition according to the present invention is 0.001 to 1000 mg / Kg · day.

The compositions according to the invention can be administered alone or in combination with other agents or together to assist other agents.

In addition, in the external preparation composition of each formulation, other components other than the above-mentioned essential components can be suitably selected and blended by those skilled in the art according to the formulation of the external preparation or the purpose of use.

Hereinafter, the configuration and effect of the present invention will be described in more detail with reference to Examples and Test Examples. However, the present invention is not limited only to these examples.

Example

Example 1 Preparation of Plantain Outpost Extract

After adding 70% ethanol of 4 times the weight (Kg) ratio to 500 g of plantain outpost, and heated at 80 C for 2 hours to extract the active ingredient. This was allowed to stand at room temperature for 1 day to precipitate impurities, and the filtrate was filtered twice using filter paper and concentrated by completely evaporating using a reduced pressure evaporator.

Example 2 Preparation of Plantain Seed Extract

After adding 70% ethanol of 4 times the weight (Kg) ratio to 500 g of plantain seed, it was heated at 80 C for 2 hours to extract the active ingredient. This was allowed to stand at room temperature for 1 day to precipitate impurities, and the filtrate was filtered twice using a filter paper and concentrated by completely evaporating using a reduced pressure evaporator.

Test Example 1 Measurement of Proliferation Effect of Skin Cells

The proliferative effect of keratinocytes was measured by treating the skin keratinocyte extracts prepared in Examples 1 and 2 directly with human dermal keratinocytes obtained directly from humans or purchased commercially. That is, put human keratinocytes into 5,000 cells / well in a 96-well microtiter plate containing KGM (Keratinocyte Growth Media) containing 1.0% fetal bovine serum and attach cells. Afterwards, the fetal bovine serum-free KGM medium was changed and cultured to 70 80% growth. Then, the treatment was carried out according to the concentration of Table 1, after 3 days to measure the number of living cells, to observe the proliferative effect on the skin cells. At this time, the group not treated with the composition was used as a control. The results are shown in Table 1 comparing the control to 100.

ingredient Concentration (ppm) Cell number (%) Example 1 10 198 One 164 0.1 142 Control 100 Example 2 10 211 One 178 0.1 156 Control 100

As shown in the table, it can be seen that the plantain outpost extract or plantain seed extract proliferates keratinocytes without causing toxicity to skin cells, thereby thickening the thickness of the epidermis. If the skin becomes thinner, it may cause wrinkles to form immediately. Therefore, if the skin is thickened it can prevent or improve the wrinkles.

Test Example 2 Measurement of Collagen Biosynthesis Efficacy of Skin Cells

After culturing human fibroblasts in a 48-hole plate incubator at a concentration of 10 ⁴ , each extract according to Examples 1 and 2 was replaced with a medium containing 0.1 ppm, 1 ppm, and 10 ppm. After 24 hours of culture, the supernatant was harvested and the amount of procollagen produced was quantified using the ELISA (Takara MK101) method. The results are shown in Table 2 comparing the control to 100.

Concentration (ppm) Collagen Biosynthesis (%) Example 1 Example 2 10 134 139 One 129 132 0.1 116 120 Control 100 100

From Table 2, it can be seen that the plantain outpost extract or plantain seed extract increases the collagen biosynthesis in a concentration-dependent manner. In the above composition, it can be seen that the plantain seed extract is particularly excellent in collagen biosynthesis.

Test Example 3 Measurement of the Biosynthesis Inhibitory Effect of Type I Collagenase (MMP-1) by Ultraviolet Rays

Human fibroblasts cultured in ^10-4 concentration in the incubator 48, Plate and, after irradiating the ultraviolet A after 24 hours to 15J / cm ^2, the first and second embodiments to extract the 0.1pp, 1ppm, including 10ppm according Replaced with medium. Supernatants were harvested on day 2 of culture (harvest) to quantify the amount of type I collagenase produced using the ELISA (AP biotech RPN2610) method. The results are shown in Table 3 comparing the UV control group to 100.

Concentration (ppm) Type I collagenase biosynthesis (%) Example 1 Example 2 10 34 31 One 56 49 0.1 80 69 Control 100 100

From Table 3, it can be seen that the plantain outpost extract or plantain seed extract reduces the biosynthesis of type I collagenase by UV concentration in a concentration-dependent manner. In particular, it can be seen that the plantain seed extract is very effective in inhibiting the type I collagenase biosynthesis can effectively prevent the degradation of the skin substrate.

Test Example 4 Determination of Biosynthesis Inhibition Effect of Cyclooxygenase-2 by Ultraviolet Rays

After the human fibroblasts cultured in 10 ^five concentrations in 12-plate culture medium, and irradiated with a UV-A 15J / cm ^2, to replace the extract according to Example 1 and Example 2 in medium containing 0.1pp, 1ppm, 10ppm It was. Cells were harvested on the second day of culture (harvest) and the amount of cyclooxygenase-2 (COX-2) produced using the Western blot method was quantified. The results are shown in Table 4, a comparison value measured with a densitometer with a UV control of 100.

Concentration (ppm) COX-2 biosynthesis (%) Example 1 Example 2 10 16 10 One 39 32 0.1 75 64 Control 100 100

From Table 4, the plantain outpost extract or plantain seed extract reduces the concentration of the cyclooxygenase-2 biosynthesis by ultraviolet light to reduce skin redness, pain, and degradation by prostaglandin E2 (PGE2), a product of COX-2. It can be seen that it can prevent.

Test Example 5 Measurement of the Inhibitory Effect of Biosynthesis on Tumor Necrosis Factor Alpha by Ultraviolet Rays

Human keratinocytes were cultured in a 12-hole plate incubator at a concentration of 10 ⁵ , UVB was irradiated at 30mJ / cm ² , and the extracts of Examples 1 and 2 were replaced with a medium containing 0.1pp, 1ppm, and 10ppm. It was. Cells were harvested after 6-24 hours of culture (harvest) to quantify the amount of tumor necrosis factor alpha (TNF α) produced using the ELISA (Pharmingen 555212) method. The results are shown in Table 5 comparing the UV control group to 100.

Concentration (ppm) TNF α biosynthesis (%) Example 1 Example 2 10 22 16 One 43 35 0.1 74 65 Control 100 100

From Table 5, the plantain outpost extract or plantain seed extract reduces the biosynthesis of tumor necrosis factor alpha due to the concentration, depending on the concentration, skin inflammation that can occur due to ultraviolet rays, reduction of skin matrix material, decrease in elasticity, wrinkle formation, etc. It can be seen that can be effectively prevented or improved.

Test Example 6 Determination of Biosynthesis Inhibitory Effect of Type I Collagenase (MMP-1) by Microinflammatory Factors

Human fibroblasts were cultured in a 48-hole plate incubator at a concentration of 10 ⁴ , and after 24 hours, tumor necrosis factor alpha (TNF α, Tumor necrosis factor α) was 20ng / ml, according to Examples 1 and 2 above. Extracts were replaced with medium containing 0.1ppm, 1ppm, 10ppm. Supernatants were harvested on day 2 of culture (harvest) to quantify the amount of type I collagenase produced using the ELISA (AP biotech RPN2610) method. The results are shown in Table 6 comparing the TNF α control group to 100.

Concentration (ppm) Type I collagenase biosynthesis (%) Example 1 Example 2 10 44 37 One 66 56 0.1 86 77 Control 100 100

From Table 6, it can be seen that the plantain outpost extract or plantain seed extract reduces the concentration of biosynthesis of type I collagenase by TNF α which is a microinflammatory factor. In particular, it can be seen that the plantain seed extract is very effective in inhibiting the type I collagenase biosynthesis can effectively prevent the degradation of the skin substrate.

Test Example 7 Measurement of Inhibitory Effect of Biosynthesis of Cyclooxygenase-2 by Microinflammatory Factors

Human fibroblasts were cultured in a 12-hole plate incubator at a concentration of 10 ⁵ , and after 24 hours, the tumor necrosis factor alpha (TNF α, Tumor necrosis factor α) was 20ng / ml, and Example 1 and Example 2 were 0.1pp, 1ppm, 10ppm containing medium was replaced. Cells were harvested on day 2 of culture (harvest) and the amount of cyclooxygenase-2 (COX-2) produced using the Western blot method was quantified. The result is shown in Table 7 the comparison value measured with a densitometer (control group as 100).

Concentration (ppm) COX-2 biosynthesis (%) Example 1 Example 2 10 15 11 One 38 31 0.1 72 62 Control 100 100

From Table 7, the plantain starch extract or plantain seed extract reduces the biosynthesis of cyclooxygenase-2 by microinflammatory factors in a concentration-dependent manner, resulting in skin redness, pain, and pain caused by prostaglandin E2 (PGE2), a product of COX-2. It can be seen that decomposition can be prevented.

Test Example 8 Measurement of Collagen Degradation Inhibition Effect by Micro-inflammatory Factor

Human fibroblasts were incubated in a 48-hole plate incubator at a concentration of 10 ⁴ , and after 24 hours, tumor necrosis factor alpha (TNF α, Tumor necrosis factor α) was added to 20 ng / ml, and the extract of Example 1 or 2 was used. Was replaced with a medium containing 0.1ppm, 1ppm, 10ppm. Cells were harvested on day 2 of culture (harvest) and the amount of procollagen remaining undigested was quantified using the ELISA (Takara MK101) method. The results are shown in Table 8 comparing the measured value with the control group 100.

Concentration (ppm) Procollagen amount (%) Example 1 Example 2 10 100 100 One 92 94 0.1 85 88 TNF α 50 50 Control 100 100

From Table 8, it can be seen that the plantain outpost extract and plantain seed extract can effectively control the decomposition of collagen by TNF α and control in a concentration-dependent manner.

Test Example 9 Measurement of Epidermal Thickness in Hairless Mice

In order to confirm the change of the skin thickness by the composition of the present invention, the composition according to the present invention (Examples 3 and 4) was prepared as shown in Table 9, and compared with Examples 3 and 4 on the back of the hairless mice. Examples were applied for a week and then biopsied to determine the thickness of the skin epidermis by H & E staining.

Ingredient Example 3 (%) Example 4 (%) Comparative example (%) Purified water To 100 To 100 To 100 Plantain Outpost Extract (Example 1) 0.1 - - Plantain seed extract (Example 2) - 0.1 - Vegetable Cured Oil 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 Glycerol Stearate 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 1.00 Arakidil Behenyl Alcohol & Arakidil Glucoside 1.00 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 2.00 PEG-100 Stearate & Glycerolate & Propylene Glycol 1.50 1.50 1.50 Caprylic / Carric Triglycerides 11.00 11.00 11.00 Cyclomedicon 6.00 6.00 6.00 Preservative, incense Quantity Quantity Quantity Triethanol amine 0.1 0.1 0.1

The results are shown in Figures 1a to 1c. Figure 1a shows a control group treated with a composition containing no plantain starch extract or plantain seed extract, Figure 1b is an experimental group treated with a composition containing plantain starch extract, Figure 1c is treated with a composition containing plantain seed extract One experimental group is shown. As shown in Figures 1a to 1c, when the plantain starch extract or plantain seed extract is applied to the epidermis thickening 2-3 times more than the comparative example, it can be observed that the dermis is significantly thicker and higher density, which is the skin It is to prove that it can reduce the fine wrinkles and strengthen the elasticity.

Test Example 10 Collagen Biosynthesis and Wrinkle Improvement Effect in Hairless Mice (Measurement of Epidermal Thickness)

In order to confirm the effect of skin collagen synthesis by the composition of the present invention, as shown in Table 9, Examples 3 and 4 and Comparative Example were applied to the back of the hairless mice for one week, and then biopsied to perform Van Gieson staining. It was measured by. The results are shown in Figures 2a to 2c.

Figure 2a shows a control group treated with a composition containing no plantain starch extract or plantain seed extract, Figure 2b is an experimental group treated with a composition containing plantain starch extract, Figure 2c is treated with a composition containing a plantain seed extract One experimental group is shown.

As shown in Figures 2a to 2c, it was observed that when the composition containing plantain starch extract or plantain seed extract is applied, the biosynthesis amount of dermal collagen increases and the density is higher than that of the comparative example.

In addition, in order to confirm the effect of improving wrinkle formation in vivo 60 hairless mice were raised in a constant temperature and humidity room, and divided into three groups of 20 animals in each group. Each of the compositions of Examples 3, 4 and Comparative Examples was applied to the back region daily for 8 weeks, followed by a replica of the back region using silicone, followed by an image analyzer (SVI600; Courage + Khazaka electronic). GmbH, Germany) was used to evaluate the depth, area of wrinkles, the degree of wrinkle improvement and the results are shown in FIG.

In Figure 3, it can be seen that the wrinkles appearing vertically deep in the comparative example is very excellent in Examples 3 and 4.

Test Example 11 Wrinkle Improvement Effect in Humans

The comparative examples and Examples 3 and 4 of Table 9 were evaluated as follows to confirm the wrinkle improvement effect. Sixty women in their thirties were divided into three groups of 20 people each of Comparative Example, Example 3, and Group 4, and applied once a day for 8 weeks, and then, using a silicone, the replica was scooped up and the visiometer (SV600, Courage + Khazaka electronic GmbH, Germany) and image analysis. The results are shown in the table below. The values in the table below represent the average of each parameter value after 8 weeks of application minus the parameter value before application.

Clinical results after 8 weeks of use R1 R2 R3 R4 R5 Comparative example 0.28 0.27 0.22 0.04 0.04 Example 3 -0.21 -0.20 -0.12 -0.04 -0.03 Example 4 -0.22 -0.20 -0.13 -0.04 -0.03

R1: difference between the highest and lowest of the wrinkle contour

R2: Divide the wrinkle contours by 5 spaces, and then average the R1 values

R3: Maximum value of R1 divided by 5

R4: The mean value of the baseline of the wrinkle contour, minus the top and valley values of each angle

R5: Difference value of the baseline of the wrinkle contour line minus each wrinkle outline

As shown in Table 10, the external preparation composition of Examples 3 and 4 was found to have a very good skin wrinkle improvement effect.

Test Example 12 Measurement of Tropoelastin and Fibrillin Production in Human Skin Fibroblasts

Whether planar outpost extract or plantain seed extract increased the production of tropoelastin and fibrin, which play an important role in elasticity in human skin, was measured by the following method.

Fibroblasts isolated from human skin were placed in each well of a 24-well cell culture plate and attached 5 × 10 ⁴ for 24 hours. After attaching the test material to the concentrations in Table 11 below, after 2 days, the medium was harvested. Enzyme immunoassay (ELISA) was used to quantify tropoelastin and fibrin in the harvested medium. The control group was reported as 100 and expressed as a relative ratio.

Test substance Concentration (ppm) Tropoelastin Production (%) Fibrillin production (%) Plantain Outpost Extract (Example 1) 10 135 133 One 127 125 0.1 118 116 Plantain seed extract (Example 2) 10 140 141 One 130 129 0.1 12 121 Control 100 100

As shown in the table, depending on the concentration of each test substance, it was found that the tropoelastin and fibrillin were synthesized much when treated with plantain starch extract or plantain seed extract compared to the control group.

Test Example 13 Measurement of Skin Elasticity Improvement in Human Body

The skin elasticity improving effect of the lotion prepared as shown in Table 9 was measured. Thirty healthy women aged 30 years or older at a temperature of 24-26 ° C. and a humidity of 75% were divided into three sets, and each set of facial lotions of Comparative Example and Example 3 and Example 4 of Table 9 was applied twice a day for 12 weeks. After application, skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany). The results are shown in Table 12. The results in Table 12 are described as ΔR8 (R8 (left) -R8 (right)) values of Cutometer SEM 575, where R8 values represent the nature of skin viscoelasticity. Separately, at the end of the test, the subjects were asked to fill out a questionnaire, which was evaluated mechanically and subjectively. The results are shown in Table 13 below.

Experimental product Skin elasticity effect Comparative example 0.11 Example 3 0.43 Example 4 0.44

As shown in Table 12, in the group coated with the compositions of Examples 3 and 4 containing the plantain outpost extract or plantain seed extract was increased skin elasticity compared to the group applied to the comparative example.

On the other hand, it was confirmed through the survey that the skin elasticity is improved when applying Examples 3 and 4 of the composition according to the present invention [Table 13].

Applicator Respondents (Personal) Very good Good usually Inadequate Comparative example One 3 3 3 Example 3 3 5 2 0 Example 4 3 6 One 0

Test Example 14 Measurement of Glycosaminoglycan Production in Human Skin Fibroblasts

Glycosaminoglycans (GAGs), which serve to fill the rest of the skin other than collagen and elastin, support the skin, and hold moisture in the dermis, were quantified. First, human fibroblasts were cultured by placing 5 × 10 ⁴ cells in a 24-well cell culture plate using 5% fetal bovine serum treated with phenol red-containing medium and charcoal (charcoal). After 18 hours, the test material was treated to the concentration shown in Table 14, and after 18 hours, the amount of GAG secreted in the supernatant was measured. For analysis, Blyscan Glycosaminoglycan assay kit manufactured by Biocolor, UK was used. The principle of GAG measurement is the binding of 1,9-dimethyl methylene blue with positive charge to sulfated GAG and proteoglycan to precipitate and to dissolve and quantify the precipitate. The results in the table below are reported as the control group 100 and expressed as a relative ratio.

Test substance Concentration (ppm) Glycosaminoglycan Biosynthesis (%) Plantain Outpost Extract (Example 1) 10 145 One 134 0.1 123 Plantain seed extract (Example 2) 10 148 One 137 0.1 129 Control 100

As shown in the table, it was found that the glycosaminoglycans were synthesized more than the control group when the plantain outpost and the plantain seed extract were treated.

Test Example 15 Measurement of Skin Moisturizing Increase in Human Body

Table 9 and the skin moisturizing effect of the prepared lotion was measured. Thirty adult men and women in their 50s and 60s showing skin dryness were divided into three groups, and the skin lotion of Comparative Example of Table 9, and Examples 3 and 4 was applied to the face twice daily for 4 weeks. Coniometer at constant temperature and humidity conditions (24 ° C, 40% relative humidity) before start of application, after 1 week, 2 weeks, 4 weeks after application, and after 2 weeks (total 6 weeks) after application was stopped. Skin moisture content was measured by. The results are shown in the table below. The results in the table below show the percentage increase in measured values after treatment for a period of time based on the Koniometer values measured immediately prior to the start of the test. The Koniometer is a skin moisture meter that measures the amount of moisture present on the skin by measuring the conductivity of the epidermis. Separately, at the end of the test, the subjects were asked to fill out a questionnaire, which was evaluated mechanically and subjectively.

Applicator Moisture growth rate (%) 1 week past 2 weeks past 4 weeks past After 6 weeks Comparative example 32 35 33 13 Example 3 43 48 54 46 Example 4 44 49 56 47

As shown in the above table, in the group coated with the material of Examples 3 and 4 containing plantain starch extract or plantain seed extract, the skin moisture content was increased more than the comparative application group, and the test substance After 2 weeks of discontinuation of application of the skin, the value after 6 weeks of skin moisture measurement was similar to that after 1 to 2 weeks. Skin moisture was found to be maintained continuously.

At the same time, through a questionnaire on the improvement of skin dryness, it was confirmed that the application of Examples 3 and 4 containing plantain outpost extract or plantain seed extract improved skin dryness [Table 16].

Applicator Respondents (Personal) Very good Good usually Inadequate Comparative example One 4 3 2 Example 3 3 5 2 0 Example 4 3 6 One 0

Hereinafter, on the basis of the results of the above test example, by containing at least one of the plantain outpost extract and plantain seed extract, to improve the skin wrinkles and elasticity, to provide a composition of the cosmetics of various formulations to moisturize the skin. However, the composition of the present invention is not limited only to the following examples.

Formulation Example 1 Flexible Cosmetic (Skin Lotion)

Ingredient weight% Purified water To 100 Plantain Outpost Extract and / or Plantain Seed Extract 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity

Formulation Example 2 Nutritious Longevity (Milk Lotion)

Ingredient weight% Purified water To 100 Plantain Outpost Extract and / or Plantain Seed Extract 0.1 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / Capric Triglycerides 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

Formulation Example 3 Nutrition Cream

Ingredient weight% Purified water To 100 Plantain Outpost Extract and / or Plantain Seed Extract 0.1 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / Capric Triglycerides 5.0 glycerin 5.0 Putylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

Formulation Example 4 Massage Cream

Ingredient weight% Purified water To 100 Plantain Outpost Extract and / or Plantain Seed Extract 0.1 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / Capric Triglycerides 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

Formulation Example 5 Pack

Ingredient weight% Purified water To 100 Plantain Outpost Extract and / or Plantain Seed Extract 0.1 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Pingzi-12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, flavoring Quantity

As described above, by containing at least one of the plantain outpost extract and plantain seed extract, it controls the micro-inflammatory state of the skin, adjusts the skin thickness, maintains the elastic and healthy skin condition, and also collagen, elastin in the dermis Increasing the biosynthesis of glycosaminoglycans, such as wrinkles and elasticity recovery can be exhibited. In addition, the skin can be kept moist to prevent wrinkles and prevent and improve skin dryness.

Claims (12)

An external preparation composition for moisturizing skin, comprising a plantain extract. According to claim 1, wherein the plantain extract is an external composition for moisturizing the skin, characterized in that the extract of Plantago asiatica ( Plantago Esiatica ). The external preparation composition for moisturizing skin according to claim 1, wherein the plantain extract is an extract of plantain seed. The external preparation composition for moisturizing skin according to claim 1, wherein the plantain extract is an extract of plantain outpost. The external preparation composition for moisturizing skin according to claim 1, wherein the plantain extract is a mixture of an extract of plantain seed and an extract of plantain outpost. The external preparation composition for moisturizing skin according to claim 1, wherein the plantain extract is contained in an amount of 0.001 to 10% by weight in the total composition. The external preparation composition for moisturizing skin according to claim 6, wherein the plantain extract is contained in an amount of 0.01 to 5% by weight in the total composition. delete delete delete delete delete

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