KR101229062B1 - Composition against striae distensae with the extract of momordica charantia l - Google Patents

Abstract

PURPOSE: A composition containing a Momordica charantia L. extract for promoting collagen synthesis is provided to prevent and treat striae distensae and to be used as a cosmetic product or an external use formulation. CONSTITUTION: A cosmetic composition for preventing or treating striae distensae contains 0.000001-90.0 wt% of a Momordica charantia L. extract as an active ingredient. The cosmetic composition is lotion, gel, water soluble liquid, cream, essence, an oil-in-water type or water-in-oil type basic skin care formulation, oil-in-water type or water-in-oil type make-up base, foundation, skin cover, lip stick, lip gross, face powder, eye shadow, cheek color, or eyebrow pencil. An external use composition for preventing or treating striae distensae contains the extract as an active ingredient.

Description

Composition against striae distensae with the extract of Momordica charantia L.

The present invention relates to a composition for preventing or improving stretch marks, and more particularly, to a composition for preventing or improving swelling swelling, which includes an extract of gourd.

The skin surrounding the human body is a very elastic tissue, but if the subcutaneous body tissues, such as muscles or fats, suddenly quantitatively swell, the skin can tear without keeping up with the rate of expansion. The thighs and abdomen, such as the skin is not able to keep up with the expansion rate of the subcutaneous body tissues, thin cracking or tearing is often called 'stretched'.

Stretch marks are also commonly referred to as stretch marks, which in medical terms are called 'striae distensae' or 'swelling gland'. Stretch marks are atrophic linear band lesions that appear on the skin of damaged areas by pulling force. In histological terms, the epidermis is atrophy and the dermis is thinned with collagen fibers and rearranged parallel to the skin. Early lesions appear to have red lines or bands on the skin, and if you look closely, they are slightly sunk than normal skin, making them feel bumpy when touched. These lesions are called purple ancestors (striae rubra). Purple ancestors gradually turn white over time, becoming less pronounced, turning into wrinkled and atrophic skin, called white ancestors (striae alba). Stretch marks usually have a reddish color when they are early or in progress, and after a long time the color changes to a white or milky color, and the white stretch marks differ from the normal skin color around them. As a result, the appearance of the skin looks ugly.

Stretch marks are most common in the areas of arms, thighs, abdomen, buttocks, and furrows, but they can also occur in areas such as the face, and stretch marks due to Cushing's syndrome may occur on the skin of the folding area. In addition, stretch marks from pregnancy are common in the abdomen and breast. Stretch marks in obese patients tend to be less pronounced, less atrophic, and narrower than stretch marks in Cushing's syndrome patients. On large stretch marks, rarely ulcers or cracking of the skin occur.

On the other hand, stretch marks may be caused by an increase in corticosteroids in the body due to physiological or pathological factors, resulting in the destruction of collagen and elastin fibers in the dermal layer, or the failure of the collagen to respond to rapid muscle growth. have. In other words, stretch marks are proteins belonging to collagen due to physiological and physical factors. Collagen fibers in the dermal dermal layer, which is the main component of skin connective tissue, are destroyed or elastin is lost. It was expressed.

Types of stretch marks include corticosteroids secreted to protect the fetus during pregnancy, along with the expansion of the skin due to the growth of the fetus or the abdominal cortex caused by pregnancy, which sometimes occurs as a result of abrupt ablation of the increased belly after childbirth. There are stretch marks in women due to excessive secretion of hormones, stretch marks caused by obesity and diet, endocrine diseases, chronic wasting diseases, stretch marks caused by long-term use of steroid preparations, and stretch marks caused by excessive muscle movement or stretching. In medicine, the "improvement effect" is more appropriate than the word "treatment" because it is almost impossible to recover the prototype.

Stretch marks are more likely to be treated early on, but after turning white, it is difficult to expect a satisfactory therapeutic effect. Conventionally, there was a method of treating stretch marks in the early stages using a Versapulse laser, or applying retinoid acid to the site of the development of the swelling line. In addition, as a method of treating the swelling of the late stage, retinoic acid is applied for several months, and the epidermis is peeled thinly using an ultrapulse CO ₂ laser or Erbium-YAG laser. There has been a method of stimulating collagen fibroblasts to promote collagen remodeling. However, the conventional swelling treatment method of the conventional swelling takes about 6 months to 1 year, it is difficult to show more than 50% improvement even if the treatment, in some cases may cause side effects such as erythema, pigmentation.

In the meantime, Momordica charantia L.) is a gourd and plant of the night tree, with leaves alternate and long sacks. Petiole split into 5 ~ 7 teeth with sawtooth. The fruit is oval shaped and covered with bumps. It is a vine and grows well in hot and humid climates. It is tropical in Asia and planted for ornamental purposes. The stem is thin and grows 1 ~ 3m in length and winds up with other things with tendrils. Leaves are alternate, long sack, 5-7 edges split, forked pieces are serrated. Fruits are gourds, long oval, narrow at both ends, hump-like projections, and ripened to reddish red and split irregularly to give red flesh-like seeds. The fruit is similar to yeoji, so it is called yeoju. The young fruit and red seedling (種 皮) is for food and the seeds are for medical use. Also used as an ornamental.

Yeoju contains 5 times more vitamin C than mature cucumber, 10 times more immature Yeoju, and 19 times more yeast grass. It contains various vitamins and minerals in addition to vitamin C. It contains amino acids, saponins, and fatty acids. Tic acid, stearic acid, linoleic acid, oleic acid), and the like.

This study has been discussed mainly on the anti-diabetic effect to date, and it has not been determined whether it can prevent or improve stretch marks as external application for skin.

Methods for treating or preventing stretch marks include Republic of Korea Patent No. 10-656807, "Composition for preventing and treating swelling filigree consisting of citric acid, zinc and arginine" and Republic of Korea Patent Publication No. 10-2001-0097020, "Dose quantification The composition for the treatment of swelling filigree containing the extract ”is disclosed. In addition, International Publication No. WO2003 / 004043 discloses various scar and swelling streak compositions containing onion extracts.

The present invention is to develop and provide an external composition for skin having the effect of preventing and improving swelling ancestry using the extract of gourd that is a gourd.

The present invention is Momordica It provides a cosmetic composition for preventing or improving stretch marks, characterized in that the charantia L.) extract as an active ingredient. The invention also gourd (Momordica It provides an external preparation composition for preventing or improving swelling filigree, characterized in that it contains charantia L.) extract as an active ingredient.

On the other hand, in the composition of the present invention, the cosmetic composition, for example, the base cosmetic formulation consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type. ; Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek collars and eyebrow pencils; It may be any one selected from.

On the other hand, in the composition of the present invention, the external composition is, for example, PLASTERS, lotion (LOTIONS), linings (LINIMENTS), liquid (LIQUIDS AND SOLUTIONS), aerosol (AEROSOLS), X EXTRACTS, OINTMENTS, FLUIDEXTRACTS, EMULSIONS, SUSPENSIONS, CAPSULES, CREAMS, soft or hard gelatin capsules, patches, sustained release agents It may be any one selected from among the above.

On the other hand, in the composition of the present invention, the bitter gourd extract may be preferably added 0.000001 ~ 90.0% by weight based on the dry weight of the total composition.

On the other hand, in the composition of the present invention, the bitter gourd extract may preferably be obtained by using an ethanol aqueous solution as the extraction solvent.

Hereinafter, the problem solving means of the present invention will be described in detail.

The present invention in order to provide a cosmetic composition or external preparation composition excellent in the prevention and improvement effect of swelling ancestors, Momordica charantia L.) It is characterized by containing the extract as an active ingredient. Yeoju extract used in the present invention can be obtained by extracting from the fruit, seeds of Yeoju. At this time, the extraction method of gourd may use a method commonly used in the art to which the present invention belongs. For example, about 2 to 20 times, preferably about 2 to 5 times water, methanol or ethanol, and the like (C ₁ -C ₄₎ to the powder obtained by freeze-drying and grinding the fruit and seeds of Yeoju A polar solvent of alcohol or a mixed solvent thereof may be extracted by applying extraction methods such as cold needle extraction, reflux cooling extraction, hot water extraction, ultrasonic extraction, and ultrahigh pressure extraction at an extraction temperature of 10 to 30 ° C. In addition, preferably, the extract obtained in this way can be used after filtration, concentration under reduced pressure or lyophilization. Moreover, it can also be soluble in a polar solvent as needed. For example, the obtained extract is filtered and then transferred to a concentration tank, concentrated under reduced pressure and lyophilized at 50 ° C. or lower, and the purified water, ethanol, The extract used in the present invention may be prepared by adding at least one solvent selected from butylene glycol and propylene glycol.

Yeochi extract of the present invention obtained as described above is very effective in promoting collagen biosynthesis effect, elastin production, skin moisturizing effect, swelling prophylaxis and improvement effect can be used as a multifunctional skin external preparation composition Was confirmed.

On the other hand, the topical skin composition of the present invention preferably contains 0.000001 to 90.0% by weight of the total extract of the composition, if less than 0.000001% by weight of the skin improvement effect is insignificant, when it exceeds 90.0% by weight This is because it is not economical because the efficiency is inferior to the material input amount.

Meanwhile, in the present invention, "contained as an active ingredient" means that the extract of bitter gourd is added to the composition for external application for skin to the extent that it can show the effect of preventing and inhibiting stretch marks from the external preparation for skin of the present invention. And, for drug delivery and stabilization, etc. are added to the various components as a subcomponent means to include the formulation (formulation) in various forms.

On the other hand, the composition of the present invention may preferably be a cosmetic composition, the formulation of the cosmetic composition, for example, lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) Basic cosmetic formulations; Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek collars and eyebrow pencils; Etc.

On the other hand, the composition of the present invention may preferably be a topical skin composition, the formulation of the topical composition, for example, PLASTERS, lotion (LOTIONS), linings (LINIMENTS), liquids (LIQUIDS AND SOLUTIONS), AEROSOLS, EXTRACTS, Ointment, FLUIDEXTRACTS, Emulsion, Emulsion, SUSPESIONS, CAPSULES, CREAMS, Soft or hard gelatin Capsules, patches, sustained-release agents.

The external preparation composition of the present invention may be a pharmacologically acceptable base; Carrier; Excipients; Binders including starch, tragacanth gum, gelatin, molasses, polyvinyl alcohol, polyvinyl ether, polyvinyl pyrrolidone, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose and carboxymethyl cellulose; Grinding agents including agar, starch, gelatin powder, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, crystalline cellulose, calcium carbonate, sodium bicarbonate and sodium alginate; Lubricants including magnesium stearate, talc and hydrogenated vegetable oils; And a coloring agent. Examples of the carrier and excipient include lactose, glucose, sucrose, mannitol, potato starch, cornstarch, calcium carbonate, calcium phosphate, and cellulose. In addition to the above, additives such as stabilizers, solubilizers, perfume fragrances such as transdermal absorption accelerators, and preservatives may be further added.

On the other hand, the standard dosage of the external preparation composition of the present invention may be varied in amount by the individual characteristics of the patient, and a practically skilled clinician may determine the ideal dosage and administration of the external skin composition of the present invention for the patient. The most appropriate treatment strategy can be determined in terms of the plan, for example the specific needs and the overall condition of the patient. For the dermal topical composition of the present invention, a number of references can be referred to in determining an appropriate dosage.

In addition, the appropriate dosage of the external preparation composition for skin of the present invention may be generally determined in vitro or in an animal model. For example, appropriate concentrations may be determined by adding various concentrations of the topical skin composition of the invention to target cells in vitro.

Since the present invention exhibits excellent collagen synthesis promoting, skin moisturizing, and swelling prevention and improvement effects, it can be used as cosmetics or external preparations for the purpose of preventing and improving stretch marks.

Hereinafter, the present invention will be described in more detail in the following Examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[ Example  1: Preparation of Yeast Extract]

Litchi ( Momordica After washing the immature fruit and Yeoju seeds of charantia L. ), mix the immature fruit and Yeoju seeds in a weight ratio of 1: 1, and extract reflux three times for 5 hours in an aqueous solution containing 70% (v / v) of ethanol. After cooling, it was filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 ° C. or lower, and then dissolved in a mixed solvent of purified water, ethanol, butylene glycol and propylene glycol so as to contain 15% by weight of the obtained freeze-dried extract. (Hereinafter, referred to as 'yum extract') was prepared (Example 1).

[ Experimental Example  1: Determination of Promoting Type 1 Procollagen Biosynthesis]

In Experimental Example 1, TGF-β was used as a positive control group in order to confirm the effect of promoting the biosynthesis of the skin matrix component of type 1 procollagen obtained in Example 1, procollagen assay (procollagen assay) Was carried out as follows.

Human dermal fibroblasts isolated from neonatal epidermal tissue were distributed from Modern Tissue Technology (MTT, Korea) and 10% fetal calf serum (FBS) was added to DMEM / F-12 (3: 1) medium. Incubated at 1 × 10 ⁴ cell / cm ² concentration. When grown at about 70-80%, the cells were subcultured at a ratio of 1: 3, and cells cultured in 3rd to 4th passages were used for the experiment.

For experiments to measure the amount of procollagen, fibroblasts were cultured at least 90% in a 48-well plate, and then the extracts were added at a concentration of 0.02%, 0.01%, and 0.005% by weight. Afterwards, the amount of free collagen in the medium was measured using the procollagen type-1 C-peptide EIA kit (MK101, Takara, Japan).

Name of sample Procollagen biosynthesis promoting effect (%) Yew Extract 0.02 wt% 34 Yew Extract 0.01% by weight 18 Yew Extract 0.005% by weight 13 TGF-β 0.001 wt% 35

As a result of measuring the effect of promoting procollagen biosynthesis (Table 1), when 0.02% by weight of the extract was treated, procollagen biosynthesis was promoted 34%, which showed a similar effect to the cell signaling substance TGF-β.

From the above results, it was possible to confirm the procollagen biosynthesis promoting effect of the Yeoju extract, from which it was found that the present invention is excellent in the procollagen biosynthesis promoting effect.

[ Experimental Example  2: Elastase  Activity inhibitory effect measurement]

In this Experimental Example 2, in order to measure the elastin production promoting effect of the bitter gourd extract obtained in Example 1, the following experiments were carried out by treating the bitumen extract to human fibroblasts directly collected from human or commercially purchased. .

First, human fibroblasts were placed in a culture flask and incubated until about 70-80% growth. Subsequently, after treating the extract for one day, the culture medium was collected and the elastin production was measured using a commercially available elastin measuring instrument [Bieth J: Biochem. med . , 11, 350-357 (1974), Schwartz DE: J. Invest Dermatol ., 86, 63-68 (1986). That is, the activity of the elastase was measured by using the absorbance to change the color generated when the NA of the Suc- (Ala) 3 NA, which is the substrate of the elastase, was decomposed. At this time, the control group was used as a group not treated with the extract.

Name of sample Elastase activity inhibition rate (%) Control group 0 Yew Extract 0.02 wt% 28 Yew Extract 0.01% by weight 16 Yew Extract 0.005% by weight 8

As a result of measuring the inhibition rate of elastase activity (Table 2), it was confirmed that the extract of Yeoju increased the activity inhibition rate of elastase in a concentration-dependent manner. From the above results it was confirmed the excellent elasticity promoting effect of the present invention.

[ Experimental Example  3: moisture hygroscopicity measurement]

In Experimental Example 3, in order to measure the moisture hygroscopicity of the extract of the bitter gourd, the bitter gourd extract obtained in Example 1 and distilled water were respectively saturated and absorbed into the dried epidermal cells of a human directly or commercially purchased. After the amount was measured, the mass change after drying for 48 hours was measured.

The amount of water absorbed per unit of skin cell mass was compared from each measured change in mass. This experiment is a method for comparing the amount of water absorbed to the epidermal cells, and is used as one method for predicting the moisturizing effect even in the human skin.

Specifically, after the human epidermal cells were dried to make the amount of water contained per unit mass constant, the weight of each epidermal cell sample and the amount of water contained therein were measured using the Kaiser method. The epidermal cells with water content were immersed in Yeoju extract and purified water for 24 hours, and then saturated and absorbed. The epidermal cells saturated with water were taken out of the cells, weighed, and some were collected and contained in a Kaiser moisture meter. The amount of water per unit mass was measured. Thereafter, the resultant was dried under reduced pressure for 48 hours, weighed, and then a portion of the sample was collected and measured by Kaiser method to determine the amount of water per unit mass contained therein. (Moisture mg / epidermal cell mass g)

Moisture content  Initial dry state Saturation After re-drying Litchi Extract 400 820 610 Purified water 400 770 400

As a result of measuring water hygroscopicity (Table 3), it was confirmed that the epidermal cells saturated with hygroscopic extract contained much more water in the redrying process than the epidermal cells saturated with hygroscopic.

From the above results, it was possible to confirm the excellent skin hygroscopicity of the extract of Yeoju, from which the excellent moisturizing effect of the present invention was found.

[ Example  2 and Comparative example  1: containing bitter gourd extract Cosmetics  Produce]

In Example 2 was prepared a cosmetic containing the extract extract obtained in Example 1. Prepared cosmetics are in the form of a cream, the composition is shown in Table 4 below. First, the phase b) recorded in Table 4 below was heated and stored at 70 ° C., and the phase b) was added to pre-emulsion and then emulsified uniformly with a homomixer and gradually cooled to a cream (Example 2, comparison). Example 1) was prepared.

Example 2 (%) Comparative Example 1 (%)


end Stearyl alcohol 8 8 Stearic acid 2 2 Stearic acid 2 2 Squalane 4 4 2-octyldodecyl alcohol 6 6 Polyoxyethylene (25 mole addition) alcohol ester 3 3 Glyceryl monostearate Aster 2 2
I Litchi Extract 3 - Propylene glycol 5 5 Purified water Until 100 Until 100

[ Experimental Example  5: Cosmetic  Skin elasticity improvement effect]

In order to measure the skin elasticity improvement effect of the cosmetic preparations prepared in Example 2 and Comparative Example 1, Example 2 and Comparative Example 1 were respectively applied to the skin stretch marks of 20 test subjects (20 to 35 year old women). It was applied twice a day for 3 consecutive months.

In order to confirm the skin elasticity improvement effect was measured using a skin elasticity measuring instrument (cutometer SEM 575, C + K Electronic Co., Germany) before and after using the product for 2 months.

The experimental results are described in Table 5 below as R7 value of Cutometer SEM 575, where R7 value represents the nature of the viscoelasticity of the skin (n = 10, p <0.05).

Experimental product (R7) Example 2 0.28 Comparative Example 1 0.13

As a result of measuring the skin elasticity improvement effect of the cosmetic (Table 5), it was confirmed that the skin elasticity improvement effect of the experimenter who applied Example 2 containing the extract of Yeoju was superior to Comparative Example 1. From the above results, it was possible to confirm the excellent skin elasticity improving effect of the external composition for skin of the present invention.

[ Experimental Example  6: Cosmetic Swelling  Improvement effect measurement]

In order to measure the effect of improving the swelling ancestry of the cosmetics prepared in Example 2 and Comparative Example 1, Example 2 and Comparative Example 1 were respectively applied to the skin stretch marks of 20 test subjects (20 to 35 year old women). It was applied twice a day for 3 consecutive months. The improvement effect on the swelling line was evaluated by measuring chromaticity, length (cm) and width (mm).

The width and length of the stretch marks were measured (0) before the application of the composition cream to the skin, and measured at 4, 8, and 12 weeks, respectively, to evaluate the changing pattern. The change in color of the skin stretch marks was measured using a videomicroscope equipped with image anasoftware.

0 (before application) 4 weeks 8 weeks 12 Weeks Length (mm) 37.0 36.5 35.5 34.0 Width (mm) 3.8 3.6 3.4 3.1 Chromaticity 4.1 3.5 3.2 2.3

As a result of measuring the swelling improvement effect of the cosmetics (Table 6), the effect of improving the stretch marks of the experimenter who applied the Example 2 containing the extract of Licorice was 12 weeks later than before the application in the value of measuring the length and width of the stretch marks. The improvement of 8% and 18% was shown, respectively, and the result of measuring chromaticity was found to be 43.9%.

From the above results, it was confirmed that the excellent swelling improvement effect of the composition of the present invention.

Claims (8)

Litchi ( Momordica A composition for preventing or improving stretch marks, characterized in that it contains charantia L.) extract as an active ingredient.
The method of claim 1,
The cosmetic composition,
Basic cosmetic formulations consisting of lotion, gel, water soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type; Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek collars and eyebrow pencils; A stretch composition for preventing or improving stretch marks, characterized in that any one selected from.
The method of claim 1,
Litchi ( Momordica charantia L.) extract
A stretch composition for preventing or improving stretch marks, characterized in that 0.000001 to 90.0% by weight of the total composition is added on a dry weight basis.
The method of claim 1,
The bitter gourd extract,
A stretch composition for preventing or improving stretch marks, which is obtained by using an ethanol aqueous solution as an extraction solvent.
Litchi ( Momordica An external preparation composition for preventing or improving swelling filigree, characterized in that it contains charantia L.) extract as an active ingredient.
The method of claim 5,
The external preparation composition,
PLASTERS, LOTIONS, LINMENTS, LIQUIDS AND SOLUTIONS, AEROSOLS, EXTRACTS, OINTMENTS, FLUIDEXTRACTS, For preventing or improving swelling swelling, characterized in that it is any one selected from emulsions, suspensions (SUSPESIONS), capsules (CAPSULES), creams (CREAMS), soft or hard gelatin capsules, patches, sustained-release agent. External preparation composition.
The method of claim 5,
Litchi ( Momordica charantia L.) extract
An external preparation composition for preventing or improving swelling filigree, which is added in an amount of 0.000001 to 90.0% by weight based on the dry weight of the total composition.
The method of claim 5,
The bitter gourd extract,
An external preparation composition for preventing or improving dilatation of the swelling line, which is obtained by using an ethanol aqueous solution as an extraction solvent.