KR101917645B1 - Cosmetic composition comprising saccharomyces ferment filtrate, asparagus officinalis stem extract and tussilago farfara flower extract - Google Patents

Abstract

The present invention relates to Saccharomyces fermentation filtrate, Asparagus Officinalis Stem extract and Tussilago Farfara ( Coltsfoot Flower) extract as an active ingredient. The cosmetic composition may exhibit an anti-inflammatory or skin-improving effect.

Description

FIELD OF THE INVENTION The present invention relates to a cosmetic composition comprising a saccharomyces yeast fermentation filtrate, an asparagus stem extract, and a Kanto tiger flower extract,

The present invention relates to Saccharomyces fermentation filtrate, Asparagus Officinalis Stem extract and Tussilago Farfara ( Coltsfoot Flower) extract as an active ingredient. The cosmetic composition may exhibit an anti-inflammatory or skin-improving effect.

Inflammation is the immune response of the body in response to a wound or disease. Oxidative stresses such as ultraviolet rays, active oxygen, and free radicals activate inflammatory factors and cause aging of various diseases and skin. The vasoactive polypeptide, kinin, plasmin and complement, have vasodilatory and contraction and chemotaxis effects, as well as lymphokines such as interleukin-6 (IL-6) Phosphorus and arachidonic acid are responsible for inflammation. Arachidonic acid is metabolized through inflammatory mediators such as prostaglandin and leukotrienes via two pathways: cyclooxygenase or lipooxygenase, mediating a variety of inflammatory responses.

Removal of inflammation to eliminate inflammation, the action of reducing vital signs and symptoms is called anti-inflammatory. To date, there have been a number of nonsteroidal anti-inflammatory agents, including flufenamic acid, ibuprofen, benzydamine, indomethacin, prednisolone, , Dexamethasone, and the like. It is also known that allantoin, azene, hydrocortisone and the like are effective for antiinflammation. However, these materials are limited in their use due to safety of skin, .

In addition to the inflammatory reaction described above, the secretion of various hormones that regulate metabolism is reduced internally, and the function of immune cells and the activity of cells are decreased with age. Externally, ozone layer destruction increases the ultraviolet ray content in the sunlight. As the environmental pollution becomes more intense, the excessive physical and chemical stimuli and stress caused by the increase of free radicals and active harmful oxygen, And the skin is damaged by promoting the generation of spots and freckles due to melanin deposits and skin aging phenomenon.

In order to prevent skin aging and damage due to such internal and external factors, physiologically active substances obtained from various animals, plants, microorganisms and the like are added to cosmetics in order to maintain healthier and more beautiful skin, Efforts have been made to maintain skin health by promoting skin metabolism by activating cells, and thus, cosmetics and external preparations for skin have been developed.

However, these materials have limited use due to safety problems such as irritation, erythema, and redness when applied to the skin, or the effect is insignificant, and it is difficult to substantially improve the skin function. Therefore, there is a demand for development of a novel composition for external application for skin, which is safer and more effective for a living body than conventional skin external composition compositions.

Korean Patent Publication No. 10-2010-0067700 Korean Patent No. 10-1477891

It is an object of the present invention to provide a method for producing Saccharomyces fermented filtrate, Asparagus Officinalis Stem) Extracts and Kanto Flowers ( Tussilago Farfara (Coltsfoot) Flower) extract as an active ingredient.

One aspect of the present invention for achieving the above object, the MRS yeast fermentation was filtered with a water Saccharomyces (Saccharomyces Ferment Filtrate), asparagus stem (Asparagus Officinalis Stem) and Extract Kanto flowers (Tussilago Farfara (Coltsfoot) Flower) extract as an active ingredient.

In the present invention, the " Saccharomyces yeast fermentation filtrate " is a fermentation filtrate of saccharomyces, a natural yeast used for making bread or beer, and is rich in amino acids, nucleic acids, peptides, minerals and vitamins. Saccharomyces yeast fermented filtrate contains natural moisturizing factor NMF and contains amino acid 30%, nucleic acid 15%, peptide 30% or more, and the nucleic acid is an essential component of skin cell metabolism, It is said to soften the skin gently.

In the present invention, " Asparagus Officinalis Stem) "is the stem of asparagus, and asparagus is said to be extinct or tropical as a monocot plant of lily. Special ingredients include asparagin and asparaginic acid alkaloids in large quantities. It also contains minerals such as calcium, phosphorus and potassium and vitamin A · B1 · B2 · C. Asparagine, the main component of asparagus, is known to be effective in helping kidney function, promoting uric acid excretion, and destroying oxalic acid crystals in the kidneys and all muscles, thereby contributing to neuralgia and rheumatism by accumulating uric acid.

In the present invention, " Kanton flower ( Tussilago Farfara (Coltsfoot) Flower "is a perennial flower of Asteraceae. The taste is spicy, the quality is warm, and the herbal medicine has a function of circulation (jung) in the herb, so it has the characteristics of jinhae and geumdam. Especially when the cough is caused by the ruins (肺虚) It is known to be effective for abscess (肺肺 下 气). In addition, it is widely applied to seawater, asthma, and upper respiratory tract infections caused by external sensation.

The " extract " of the present invention is obtained by extracting the extract obtained by extracting the mixture of the above extracts, the asparagus stem and / or the Kanto flower mixture, the diluted or concentrated solution of the extract, And extracts of all the formulations which can be formed using the extract itself and the extract, such as the dried product obtained by the above method, the preparation or the purified product of the above extract, or a mixture thereof.

The extract of the present invention can be extracted from natural, hybrid, or mutant plants of the respective plants, and can also be extracted from plant tissue cultures.

The method for extracting the extract of the present invention is not particularly limited and may be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.

The kind of the extraction solvent used in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water, anhydrous or lower alcohol of C ₁ to C ₄ , a mixed solvent of water and lower alcohol, acetone, 1,3-butylene glycol, ethyl acetate, chloroform Or a mixture of two or more of them.

More specifically, water (or distilled water) may be used as an extraction solvent in the present invention. The extract of the asparagus stem and / or the Kanto bloom may be extracted once or more by using the solvent, and the dry extract obtained by vacuum distillation or freeze-drying or spray drying the solvent extract may be prepared. In one specific embodiment of the present invention, a mixture of asparagus stem extract, Kanto flower extract and saccharomyces yeast fermentation filtrate was prepared using water (or distilled water) (Example 1).

In the present invention, the extract obtained by hot water extraction or cold extraction is filtered to remove suspended solid particles by filtration, for example, nylon or the like, or by freeze filtration, Spray drying and the like.

Specifically, the asparagus stem extract and the Kanto floral extract may be contained at a weight ratio of 1 to 10: 1 to 10, and the saccharomyces yeast fermentation filtrate may be contained in a ratio of 0.001 wt% to 1 wt% ≪ / RTI >

In one embodiment of the present invention, the asparagus stem extract and the Kanto floral extract were mixed at a weight ratio of 5: 5 and a mixture of saccharomyces yeast fermentation filtrate and 0.5 wt% of the total mixture weight was prepared. As shown in FIG. In particular, the raw materials of all the extracts of the present invention are derived from natural materials and thus have no toxicity. They are classified as EWG (Environmental Working Group) grade 1 and have been proved to have excellent safety. Therefore, even if a specific extract is contained in excess, .

Specifically, the cosmetic composition comprising the saccharomyces yeast fermentation filtrate, asparagus stem extract, and Kanto bloom extract of the present invention as an active ingredient may be used for anti-inflammation or skin improvement.

In the present invention, " anti-inflammatory " refers to the inhibition of the production of inflammation or the elimination or promotion of the already-generated inflammation. The inflammatory reaction is a mechanism to repair and regenerate the damaged area when an invasion that brings about physical changes such as physical action, chemical substance, bacterial infection or the like is applied to the living body or tissue. Once inflammation is applied, the inflammatory reaction locally causes histamine, serotonin, Vascular active substances such as bradykinin, prostaglandin, hydroxyeicosatetraenoic acid (HETE) and leukotriene are liberated, resulting in increased vascular permeability and inflammation. Inhibition of the inflammation by inhibiting the mechanism is shown in Table 1. In the case of applying the mixture containing the filtrate of Saccharomyces yeast fermentation, the extract of asparagus stem, and the extract of Ganoderma lucidum in one embodiment of the present invention, And prostaglandin E2 production inhibitory effect (Table 2), it was confirmed that the effect of suppressing the inflammation generation mechanism was excellent.

In the present invention, " skin improvement " means a process of treating, alleviating, or alleviating damage to the skin caused by intrinsic or extrinsic factors of the skin or its effect. For the purpose of the present invention, the skin improvement may be interpreted as exhibiting the effects of promoting collagen synthesis in the skin cells and alleviating the inflammation generated in the skin, but the present invention is not limited thereto. In addition, the skin improvement may include not only anti-inflammatory effects but also skin elasticity improvement and skin wrinkle improvement.

In the present invention, " skin elasticity " is expressed by elastic fibers composed of elastin existing in the dermal layer. Such elastic fibers have a very low elastic modulus such as rubber and are easily deformed by a small force, When the force is removed, it easily returns to the original shape. In addition, the elastic fibers have a form in which microfibrils are embedded in an amorphous matrix called elastin, and elastin is a protein that is found only in elastic fibers such as desmosine and isodesmosine derived from lysine, It is a protein composed of one amino acid. These desmosins and isodesmesynes form cross-links in long peptide chains, which makes elastin behave like a rubber.

In the present invention, " skin elasticity improvement " means that elastic fibers composed of elastin are present together with collagen fibers called collagen, and elastic elasticity is maintained or increased in a state where elastin and collagen are sufficiently present.

The term " skin wrinkle " in the present invention means a skin caused by a decline in skin, which may be caused by a cause of a gene, a decrease in collagen and elastin present in the skin dermis, and an external environment.

In the present invention, " improvement of skin wrinkles " refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already formed wrinkles.

In the case of applying the mixture containing the saccharomyces yeast fermentation filtrate, the asparagus stem extract and the Kanto flower extract in the present invention, the excellent collagen synthesis increase effect (Table 3), the skin elasticity improvement effect (Table 5) Wrinkle improving effect (Table 6), it was confirmed that the skin improving effect can be substantially remarkably improved.

In particular, the composition of the present invention may be characterized in that it is transdermally delivered through fine needle aspiration.

In the present invention, " fine needle " is thinner and finer fine needle than hair, which is used to physically create a small hole in the skin to create a drug penetration path. When such a fine needle is used, a drug having a large molecular weight or a drug that is difficult to be ionized or polarized can effectively penetrate into the deep dermis in the skin.

The fine needle may be solid, hollow or soluble and is not limited thereto, and may be modified and used by using a suitable material if necessary. In addition, transdermal delivery may be performed through a method of contacting or attaching a patch or sheet having a plurality of microspheres to the skin, but the present invention is not limited thereto.

In accordance with one embodiment of the present invention, it was confirmed that the composition of the present invention is suitable for transdermal delivery through micro needle without any pain or irritation, erythema, itching, and inflammation.

In addition, in the case of delivering the composition of the present invention through micro needle, the NO production inhibitory effect (anti-inflammatory effect) (Table 9), collagen synthesis increasing effect (Table 10) (Table 11) and skin wrinkle improvement effect (Table 12). Accordingly, the composition of the present invention can exhibit a more excellent effect by being transferred through fine needle.

The cosmetic composition of the present invention can be used as a cosmetic composition in the form of a solution, a topical ointment, a cream, a foam, a nutritional lotion, a softening lotion, a pack, a soft water, a latex, a makeup base, But are not limited to, those selected from the group consisting of solutions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powdered foundations, emulsion foundations, wax foundations, patches and sprays no.

The cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a cosmetic composition for general skin. Examples of the cosmetic composition include ordinary ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, , A chelating agent, a coloring agent, a preservative, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier contained in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulations of the present invention are ointments, pastes, creams or gels, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like may be used as a carrier component. In particular, But are not limited to, propellants such as rocaborn, propane / butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto. These may be used alone or in combination of two or more.

Specifically, in the cosmetic composition as described above, the saccharomyces yeast fermentation filtrate, the asparagus stem extract and the Kanto floral extract may be contained in an amount of 0.005 to 30.0% by weight based on the total weight of the cosmetic composition. And more specifically from 0.01% to 20% by weight. There is an advantage that excellent antiinflammation or skin improvement effect is exhibited within the above range, and there is an advantage that the formulation of the composition is stabilized.

In addition, the composition of the present invention can be used as a transdermal administration method such as direct application or spraying on the skin, and the administration route of the composition of the present invention can be administered through any ordinary route as long as it can reach the target tissue.

The amount of the composition of the present invention can be appropriately adjusted according to individual differences such as age, degree of lesion, and the like, and can be used for one week to several months after once to several times daily application.

The cosmetic composition comprising the saccharomyces yeast fermentation filtrate, asparagus stem extract and Kanto bloom extract as an active ingredient of the present invention is excellent in anti-inflammatory or skin-improving effect.

In addition, since the composition of the present invention is a substance derived from a natural substance, it has no toxicity and does not cause side effects even when it is applied to human body, and thus it is highly utilized as a cosmetic composition. Particularly, all the raw materials used in the present invention are classified as EWG (Environmental Working Group) grade 1, and thus it has been proved that the raw materials are excellent in safety.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited by the following examples.

Example  One. Sakaromisses Yeast fermentation filtrate , Asparagus stem extract and Kanto flower  Preparation of mixtures containing extracts

100g dry weight of asparagus stem and Kantou flower were put into a flask and extracted with 1,000g of extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare an extract of each of asparagus stem and Kanto bloom. Each of the above extracts was mixed at a weight ratio of 5: 5, and the saccharomyces yeast fermentation filtrate was mixed so as to contain 0.5% by weight based on the total weight of the mixture.

Comparative Example  1. Preparation of asparagus stem extract

100 g of asparagus stem dry weight was put into a flask and extracted with 1,000 g of extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare an asparagus stem extract.

Comparative Example  2. Kanto flower  Preparation of extract

100 g of Kanto flower dry weight was put into a flask and extracted with 1,000 g of extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a Kanto flowering extract.

Experimental Example  1. Identification of anti-inflammatory effect

1-1. Identification of NO production inhibition

In order to confirm the anti-inflammatory effect and the skin trouble relieving effect of Example 1, Comparative Example 1 and Comparative Example 2, inhibition of nitric oxide (NO) formation by the GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278) Experiments were conducted.

Specifically, RAW264.7 cells, macrophages of mice, were subcultured several times, placed in 24-well plates at 3 × 10 ⁵ per well, and cultured for 24 hours. Subsequently, Example 1, Comparative Example 1, and Comparative Example 2 were diluted with a concentration of 0.5% to replace the cell culture medium. At this time, L-NMMA (L-NG-Monomethylarginine), which is an inhibitor of NO production, was treated together as a positive control and cultured for 30 minutes. Lipopolysaccharide (LPS) 100 μl of the supernatant was transferred to a 96-well plate, and 100 μl of the GRIESS solution was added thereto at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the inhibitory effect of the herbal medicine extract on NO production. Table 1 shows the results. The experiment was repeated three times.

sample(%) NO production inhibition rate (%) Negative control (no treatment) - L-NMMA (10 [mu] g / ml) 38.21 Example 1 39.52 Comparative Example 1 11.93 Comparative Example 2 13.57

As shown in Table 1, it was confirmed that Example 1 exhibits superior NO production inhibitory activity similar to or better than that of L-NMMA, which is known as NO production inhibitor, as compared with Comparative Example 1 and Comparative Example 2. [

One- 2. Prostaglandins  E2 ( PGE2 ) Production inhibitory effect

In order to examine the inhibitory effect of the composition of the present invention on the production of prostaglandin E2, the following experiment was conducted (Reference: Jeffrey, KH, Anglea, SW, Zoe, S., and Rhia CM Intracellular Measurement of Prostaglandin E2: Effect of Anti- inflammatory Drugs on Cyclooxygenase Activity and Prostanoid Expression, Analytical Biochemistry, 1999, 271, 18-28). First, fibroblasts isolated from human epidermal tissue were cultured in a CO ₂ incubator for 24 hours, then replaced with a medium containing no FBS (fetal bovine serum), and cultured for 2 hours. 1 and Comparative Example 2 were prepared and treated at a concentration of 10 μg / mL, respectively, and then cultured in a CO ₂ incubator at 37 ° C. and 5% (v / v) for 2 hours.

Then, arachidonic acid was treated for 5 minutes as a prostaglandin E2 stimulating agent for 5 minutes, the cells were lysed, and the absorbance was measured at 450 nm in an ELISA reader to quantitate prostaglandin E2. The results are shown in Table 2 below.

Prostaglandin E2 (pg / 10 ⁵ cells) Negative control (no treatment) 24.2 ± 3.4 Arachidonic acid 375 ± 4.5 Arachidonic acid + Example 1 193 ± 8.0 Arachidonic acid + Comparative Example 1 277 ± 6.4 Arachidonic acid + Comparative Example 2 288 ± 5.3

As shown in Table 2, it was found that the effect of inhibiting the production of prostaglandin E2 in Example 1 was remarkably high.

Experimental Example  2. Confirmation of increased collagen synthesis

The above Example 1, Comparative Example 1, and Comparative Example 2 were added to a culture solution of human-derived fibroblasts to examine the effect of increasing the total collagen amount by promoting collagen synthesis at the cell level. The total amount of collagen was measured using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay Kit). The fibroblasts were cultured in different concentrations (% by weight) in Example 1, Comparative Example 1 and Comparative Example 2, and cultured in the same manner as described in Mossman T The cytotoxicity was assessed by measuring the median of 0.5% of the concentration below 1% without cytotoxicity. The results are shown in Table 1. %, And the degree of increase in the total amount of collagen was evaluated.

Each sample was added to the culture medium of human fibroblasts and cultured for 1 day. Then, the culture solution was taken and the degree of increase in the total amount of collagen at each concentration was measured at 450 nm using a PICP EIA kit using a spectrophotometer. For the comparison of the effects, the extent of increase in the total amount of collagen was measured in the same manner for the culture medium (negative control) of fibroblasts to which nothing had been added and the sample (positive control) added with vitamin C to a final concentration of 52.8 / / ml . The total amount of collagen was measured as the UV absorbance, and the increase rate of the total amount of collagen was calculated by the ratio of the total amount of collagen relative to the control group, and the results are summarized in Table 3 below. The experiment was repeated three times.

sample Total collagen (Absorbance) Growth rate (%) Negative control group 452 - Vitamin C (50 / / ml)
(Positive control group) 533 117.92 Example 1 566 125.22 Comparative Example 1 512 113.27 Comparative Example 2 518 114.60

As shown in Table 3, in Example 1, it was confirmed that the effect of promoting collagen synthesis and increasing the total amount of collagen was superior to that of vitamin C, which is a known wrinkle improving substance.

Experimental Example  3. Confirm skin elasticity improvement effect

The skin elasticity-enhancing effect of the cosmetic composition containing Example 1, Comparative Example 1 or Comparative Example 2 as an active ingredient was tested. 30 healthy women aged 20 years or older (average age 37 years) at a temperature of 24 to 26 占 폚 and a humidity of 75% were divided into three groups and were classified into three groups according to the following Table 4 including Example 1, Comparative Example 1 or Comparative Example 2 Respectively.

ingredient Experimental Example Positive control group Example 1, Comparative Example 1 or Comparative Example 2 10.0 - Vitamin C (50 / / ml) - 10.0 Cetostearyl alcohol 2.0 2.0 Chin type glyceryl stearate 1.5 1.5 Glyceryl stearate SE 1.5 1.5 Phitosqualan 4.0 4.0 Hydrogenated Polydecenes 3.0 3.0 Dimethicone 0.5 0.5 Polysorbate 60 1.0 1.0 Sorbitan sesquioleate 0.5 0.5 Methyl paraben 0.1 0.1 Propylparaben 0.1 0.1 Purified water Balance Balance Butylene glycol 5.0 5.0 Polyacrylate-13 0.5 0.5

The formulated cosmetic composition was applied for 12 weeks twice a day (morning and evening) around the eyes, and skin elasticity was measured using a skin elasticity meter (Cutometer MPA580, Conrage + Khazaka, Germany). The results of the test are shown in R8 (R8 (12 weeks) -R8 (0 weeks)) of the cutometer MPA580, and R8 values are properties of skin viscoelasticity.

division Skin elasticity improvement effect Example 1 0.88 Comparative Example 1 0.56 Comparative Example 2 0.64 Positive control group 0.91 Negative control (no treatment) 0.42

As shown in Table 5, in Example 1, the skin elasticity-improving effect was enhanced to an extent better than that of the positive control group, vitamin C.

Experimental Example  4. Confirm skin wrinkle improvement effect

The skin wrinkle-improving effect of the cosmetic material containing the above-described Example 1, Comparative Example 1 or Comparative Example 2 as an active ingredient was evaluated. The subjects were divided into three groups of 30 adult women over 30 years old, and the cosmetic composition of Table 4, which contained Example 1, Comparative Example 1 or Comparative Example 2, Uniformly applied and sufficiently absorbed. ANTERA 3D (Miravex, Ireland) was applied for evaluation of wrinkle improvement. Instrument measurement was performed before the test substance and after 4 weeks of use. The same test person measured the left part of the left eye of each subject and the same area was measured by overlapping with the image measured before the test material for the reproducibility of the measurement. The captured images were matched using ANTERA pro software, which is dedicated to ANTERA 3D, and the matched measurement sites were used for analysis.

The measured values were analyzed using the Indentation index, which is a measurement variable. Table 6 shows changes in the Wrinkles small value of the subject after 4 weeks according to the use of the cosmetic material. Wrinkles As the variation of small value increases, the effect of improving wrinkles is excellent.

division Wrinkles small value Example 1 1.77 Comparative Example 1 1.14 Comparative Example 2 1.18 Positive control (vitamin C) 1.23 Negative control (no treatment) 0.47

As shown in Table 6, it was confirmed that the wrinkle-improving effect of Example 1 was significantly superior to that of Comparative Example 1 and Comparative Example 2.

Experimental Example  4. On fine needle  by Transdermal  Check conformity

Twenty adult women aged 30 years or older were treated with ampoule prepared by mixing Example 1 according to Table 7 on the inside of the arm twice, and then infiltrated using micro-needle.

Raw material name Content (% by weight) ethanol 10 Propylene glycol 7 1,3-butylene glycol 5 Polysorbate 80 3 Salicylic acid 0.3 Nicotinamide 0.5 Dipotassium glycyrrhizate 0.5 Allantoin 0.5 Panthenol 0.5 glycerin 3 Bis-phage-18 methylethyldimethylsilane One antiseptic Suitable amount Example 1 Mixture 10 Purified water To 100

Erythema, irritation, itching, and inflammation were confirmed as the determinants of microsomal compatibility. (0: no erythema; 1: erythema but insufficient; 2: erythema but overall good; 3: erythema is severe). 4: erythema was very severe), and the extent of onset or persistence of itching after microneedle procedure was 0 to 4 (0: no itching, 1: itching but not enough, 2: itching, but overall good) (0: no inflammation, 1: inflammation but insufficient) were evaluated by visual observation through the visual observation. The degree of inflammation was evaluated according to the following criteria: 0: severe, 3: severe itching, 4: 2: inflammation but overall good 3: severe inflammation 4: very inflammatory).

Symptom Degree Pain or irritation 0.2 Erythema 0.2 Itching 0.1 Inflammation 0.1

As shown in Table 8, it was confirmed that the composition of the present invention is suitable for transdermal delivery through microspheres.

Experimental Example  5. On fine needle  Confirmation of transfer effect by

5-1. Identification of NO production inhibition

The inhibitory effect of NO production was confirmed in the same manner as in Experimental Example 1, except that the mixture of Example 1 was simply applied to skin and the case of fine needle penetration was compared.

Example 1 NO production inhibition rate (%) Simple application 39.52 Fine needle 44.89

As shown in Table 9, it was confirmed that when the mixture of Example 1 was delivered through micro needle, the NO production inhibition effect was more excellent. Particularly, in Example 1, it was confirmed through Experimental Example 1-1 that the composition itself exhibits an excellent inhibitory effect on NO formation, but the effect is further increased when it is delivered through micro needle.

5-2. Confirmation of increased collagen synthesis

The effect of increasing the collagen synthesis was confirmed by the same method as in Experimental Example 2, but the case of applying the mixture of Example 1 to the skin and the case of delivering through fine needle were comparatively confirmed. The rate of increase was calculated based on the control group of Experimental Example 2.

Example 1 Total amount of collagen (%) Growth rate (%) Simple application 566 125.22 Fine needle 598 132.30

As shown in Table 10, when the mixture of Example 1 was transferred through micro needle, it was confirmed that the collagen synthesis increase was more excellent. Particularly, in Example 1, it was confirmed through Experimental Example 2 that the composition itself exhibited an excellent effect of increasing collagen synthesis, but it was found that the effect was further increased when it was delivered through micro needle.

5-3. Skin elasticity improvement effect

The skin elasticity improvement effect was confirmed in the same manner as in Experimental Example 3, except that the case of applying the mixture of Example 1 to the skin and the case of delivering through the micro needle were comparatively confirmed.

Example 1 Skin elasticity improvement effect Simple application 0.88 Fine needle 0.97

As shown in Table 11, it was confirmed that when the mixture of Example 1 was delivered through a micro needle, the skin elasticity improving effect was more excellent. Particularly, in Example 1, it was confirmed through Experimental Example 3 that the composition exhibits excellent skin elasticity improving effect even when the composition itself is used.

5-4. Wrinkle improvement effect

The skin elasticity improvement effect was confirmed in the same manner as in Experimental Example 4, except that the mixture of Example 1 was simply applied to the skin and the case of being delivered through the micro needle was compared.

Example 1 Wrinkles small value Simple application 1.77 Fine needle 1.98

As shown in Table 12, it was confirmed that when the mixture of Example 1 was transferred through microspheres, the effect of improving skin wrinkles was more excellent. In particular, in Example 1, it was confirmed through Experimental Example 4 that the composition itself exhibited excellent skin wrinkle-improving effect, but it was found that the effect was further increased when the composition was delivered through micro needle.

Manufacturing example 1. Bonn  Preparation of an essence containing an extract of the invention

A cosmetic composition of the essence formulation was prepared as shown in Table 13 below (unit: wt%).

Raw material name Content (% by weight) Cetearyl alcohol 2 Cetearyl glucoside One Jojoba seed oil 5 Silica 0.5 glycerin 10 The extract of the present invention 5 Purified water To 100

Manufacturing example 2. Bonn  Production of a cream containing an extract of the invention

A cream form cosmetic composition was prepared as shown in Table 14 below (unit: wt%).

Composition Content (% by weight) The extract of the present invention 5 Stearic acid 15 ethanol One Potassium hydroxide 0.7 glycerin 5 Propylene glycol 3 antiseptic Suitable amount incense Suitable amount Purified water to 100

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (8)

A cosmetic composition for improving anti-inflammation and skin wrinkles, comprising Saccharomyces Ferment Filtrate, Asparagus Officinalis Stem extract and Tusilago Farfara ( Coltsfoot Flower) extract as active ingredients. The method according to claim 1,
Wherein the extract is selected from the group consisting of water, an anhydrous or lower alcohol of C ₁ to C ₄ , a mixed solvent of water and a lower alcohol, acetone, 1,3-butylene glycol, ethyl acetate and chloroform And extracting the cosmetic composition. The cosmetic composition according to claim 1, wherein the saccharomyces yeast fermentation filtrate is contained in an amount of 0.001 wt% to 1 wt% based on the total weight of the cosmetic composition. The method according to claim 1,
Wherein the saccharomyces yeast fermentation filtrate, the asparagus stem extract and the Kanto floral extract are contained in an amount of 0.005 to 30.0% by weight based on the total weight of the cosmetic composition. The method according to claim 1,
Wherein the composition is transdermally delivered through a microspheres. 6. The cosmetic composition according to claim 5, wherein the microspheres are solid, hollow or soluble. 6. The method of claim 5,
Wherein the transdermal delivery is carried out by contacting or attaching a patch or sheet with a plurality of microspheres attached thereto. delete