JP2007534308A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2007534308A5 JP2007534308A5 JP2006541541A JP2006541541A JP2007534308A5 JP 2007534308 A5 JP2007534308 A5 JP 2007534308A5 JP 2006541541 A JP2006541541 A JP 2006541541A JP 2006541541 A JP2006541541 A JP 2006541541A JP 2007534308 A5 JP2007534308 A5 JP 2007534308A5
- Authority
- JP
- Japan
- Prior art keywords
- photometrically
- labeled
- oligonucleotide
- labeling molecules
- labeling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000002372 labelling Methods 0.000 claims 6
- 108091034117 Oligonucleotide Proteins 0.000 claims 5
- 230000005284 excitation Effects 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
Description
Claims (1)
- 標識オリゴヌクレオチドであって、以下:
オリゴヌクレオチド配列;および
少なくとも2つの光度測定可能なラベリング分子であって、当該少なくとも2つの光度測定可能なラベリング分子は、互いの励起波長の15nm以内にある励起波長を有し、ここで、少なくとも2つの光度測定可能なラベリング分子は当該オリゴヌクレオチドに連結され、そして当該オリゴヌクレオチドに連結された少なくとも2つの光度測定可能なラベリング分子は互いに3〜60ヌクレオチドの範囲内にある、
を含んでなり、
ここで、前記の少なくとも2つの光度測定可能なラベリング分子の少なくとも2つが互いから引き離される時に、前記の光度測定可能なラベリング分子の少なくとも2つがシグナルの検出可能な変化を発生する、
前記標識オリゴヌクレオチド。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US52326303P | 2003-11-19 | 2003-11-19 | |
PCT/US2004/038946 WO2005051967A2 (en) | 2003-11-19 | 2004-11-19 | Oligonucleotides labeled with a plurality of fluorophores |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011177659A Division JP5307206B2 (ja) | 2003-11-19 | 2011-08-15 | 複数のフルオロフォアで標識したオリゴヌクレオチド |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2007534308A JP2007534308A (ja) | 2007-11-29 |
JP2007534308A5 true JP2007534308A5 (ja) | 2011-10-06 |
Family
ID=34632765
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006541541A Withdrawn JP2007534308A (ja) | 2003-11-19 | 2004-11-19 | 複数のフルオロフォアで標識したオリゴヌクレオチド |
JP2011177659A Expired - Fee Related JP5307206B2 (ja) | 2003-11-19 | 2011-08-15 | 複数のフルオロフォアで標識したオリゴヌクレオチド |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011177659A Expired - Fee Related JP5307206B2 (ja) | 2003-11-19 | 2011-08-15 | 複数のフルオロフォアで標識したオリゴヌクレオチド |
Country Status (5)
Country | Link |
---|---|
US (2) | US7667024B2 (ja) |
EP (1) | EP1689764B1 (ja) |
JP (2) | JP2007534308A (ja) |
CN (1) | CN101076537B (ja) |
WO (1) | WO2005051967A2 (ja) |
Families Citing this family (48)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006002167A2 (en) * | 2004-06-17 | 2006-01-05 | University Of Florida Research Foudation, Inc. | Multi-acceptor molecular probes and applications thereof |
US8685711B2 (en) | 2004-09-28 | 2014-04-01 | Singulex, Inc. | Methods and compositions for highly sensitive detection of molecules |
US9040305B2 (en) | 2004-09-28 | 2015-05-26 | Singulex, Inc. | Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry |
WO2007100392A2 (en) | 2005-11-30 | 2007-09-07 | Biotium, Inc. | Enzyme substrate comprising a functional dye and associated technology and methods |
ES2550004T3 (es) * | 2006-04-04 | 2015-11-03 | Singulex, Inc. | Sistema de alta sensibilidad y métodos de análisis de la troponina |
EP2232271B1 (en) | 2007-12-19 | 2019-09-11 | Singulex, Inc. | Scanning analyzer for single molecule detection and methods of use |
ES2594873T3 (es) | 2008-09-03 | 2016-12-23 | Quantumdx Group Limited | Métodos y kits para la secuenciación de ácido nucleico |
US10759824B2 (en) | 2008-09-03 | 2020-09-01 | Quantumdx Group Limited | Design, synthesis and use of synthetic nucleotides comprising charge mass tags |
US9579402B2 (en) * | 2009-12-04 | 2017-02-28 | Biotium, Inc. | Heterocycle-substituted xanthene dyes |
CN101709335B (zh) * | 2009-12-10 | 2011-12-21 | 浙江大学 | 一种人乳头瘤病毒常见型四重荧光定量pcr分型检测方法 |
JP5822843B2 (ja) * | 2009-12-21 | 2015-11-24 | シージーン アイエヌシー | Tsgプライマーターゲット検出 |
EP2516678A4 (en) * | 2009-12-24 | 2013-06-19 | Seegene Inc | REAL TIME MULTIPLEX DETECTION OF TARGET NUCLEIC ACID SEQUENCES WITH FALSE SIGNAL REMOVAL |
DE102010003781B4 (de) * | 2010-04-08 | 2012-08-16 | Aj Innuscreen Gmbh | Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen |
EP3369828B1 (en) | 2011-02-07 | 2020-07-15 | The Governing Council Of The University Of Toronto | Bioprobes and methods of use thereof |
JP6120449B2 (ja) * | 2011-05-06 | 2017-04-26 | キアゲン ゲーエムベーハー | リンカーを介して結合した標識を含むオリゴヌクレオチド |
JP6522339B2 (ja) | 2011-11-21 | 2019-05-29 | プロメガ コーポレイションPromega Corporation | カルボキシxローダミン類似体 |
EP4361606A3 (en) | 2012-02-03 | 2024-07-10 | California Institute of Technology | Signal encoding and decoding in multiplexed biochemical assays |
IN2015DN01609A (ja) | 2012-08-03 | 2015-07-03 | California Inst Of Techn | |
WO2014104818A1 (en) * | 2012-12-27 | 2014-07-03 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent non-hybridization assay |
WO2014116884A1 (en) | 2013-01-24 | 2014-07-31 | California Institute Of Technology | Chromophore-based characterization and detection methods |
WO2014144548A2 (en) | 2013-03-15 | 2014-09-18 | Nanobiosym, Inc. | Systems and methods for mobile device analysis of nucleic acids and proteins |
CN105377994B (zh) | 2013-08-22 | 2018-03-02 | 索尼公司 | 水溶性荧光染料或有色染料及其使用方法 |
CN106460057A (zh) * | 2014-06-02 | 2017-02-22 | 贝斯4创新公司 | 核苷酸多态性检测方法 |
CN107208147B (zh) * | 2014-11-20 | 2022-02-18 | 安普里怀斯公司 | 用于核酸扩增的组合物和方法 |
KR102676814B1 (ko) | 2015-02-26 | 2024-06-21 | 소니그룹주식회사 | 접합 그룹을 포함하는 수용성 형광 또는 착색 염료 |
WO2016138457A1 (en) | 2015-02-26 | 2016-09-01 | Sony Corporation | Phenylethynylnaphthalene dyes and methods for their use |
EP3400444A4 (en) | 2016-01-04 | 2019-10-16 | QuantuMDx Group Limited | DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES COMPRISING SPECIFIC CHARGE LABELS |
BR112018070164B1 (pt) | 2016-04-01 | 2022-09-27 | Sony Corporation Of America | Corantes diméricos ou poliméricos ultrabrilhantes |
WO2017173348A1 (en) | 2016-04-01 | 2017-10-05 | Sony Corporation | Ultra bright dimeric or polymeric dyes with rigid spacing groups |
JP7069033B2 (ja) | 2016-04-06 | 2022-05-17 | ソニーグループ株式会社 | スペーシングリンカー基を有する超明色ダイマーまたはポリマー染料 |
JP7527537B2 (ja) | 2016-05-10 | 2024-08-05 | ソニーグループ株式会社 | ペプチド骨格を有する超明色ポリマー染料 |
WO2017197014A2 (en) | 2016-05-10 | 2017-11-16 | Sony Corporation | Compositions comprising a polymeric dye and a cyclodextrin and uses thereof |
EP3455300A1 (en) | 2016-05-11 | 2019-03-20 | Sony Corporation | Ultra bright dimeric or polymeric dyes |
EP3464477A1 (en) | 2016-06-06 | 2019-04-10 | Sony Corporation | Ionic polymers comprising fluorescent or colored reporter groups |
EP3472354A4 (en) | 2016-06-17 | 2020-01-01 | California Institute of Technology | NUCLEIC ACID ACTIONS AND RELATED PROCEDURES AND COMPOSITIONS |
EP3491071A1 (en) | 2016-07-29 | 2019-06-05 | Sony Corporation | Ultra bright dimeric or polymeric dyes and methods for preparation of the same |
CN111315415A (zh) | 2017-10-05 | 2020-06-19 | 索尼公司 | 可编程的聚合药物 |
EP3710062A1 (en) | 2017-11-16 | 2020-09-23 | Sony Corporation | Programmable polymeric drugs |
WO2019140301A1 (en) | 2018-01-12 | 2019-07-18 | Sony Corporation | Polymers with rigid spacing groups comprising biologically active compounds |
KR20200135424A (ko) | 2018-03-19 | 2020-12-02 | 소니 주식회사 | 형광 신호 향상을 위한 2가 금속의 사용 |
CN118480073A (zh) | 2018-03-21 | 2024-08-13 | 索尼公司 | 具有连接体基团的聚合串联染料 |
EP3814366A1 (en) | 2018-06-27 | 2021-05-05 | Sony Corporation | Polymeric dyes with linker groups comprising deoxyribose |
WO2020154546A1 (en) * | 2019-01-23 | 2020-07-30 | Quantum-Si Incorporated | High intensity labeled reactant compositions and methods for sequencing |
WO2021004379A1 (zh) * | 2019-07-05 | 2021-01-14 | 南京金斯瑞生物科技有限公司 | 一种荧光标记核酸及其合成方法 |
EP3861074A2 (en) | 2019-09-26 | 2021-08-11 | Sony Group Corporation | Polymeric tandem dyes with linker groups |
WO2021067483A1 (en) * | 2019-09-30 | 2021-04-08 | Sony Corporation | Nucleotide probes |
US20230279479A1 (en) * | 2021-12-29 | 2023-09-07 | Co-Diagnostics, Inc. | Methods and systems for analyzing nucleic acids using increased ifret with multiple acceptor fluorophores |
US20230366038A1 (en) | 2022-05-16 | 2023-11-16 | Bioo Scientific Corporation | Compositions and methods relating to loop mediated isothermal amplification (lamp) |
Family Cites Families (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4766062A (en) | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
US5118605A (en) * | 1984-10-16 | 1992-06-02 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
US5268486A (en) | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
US5654419A (en) * | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
US5801155A (en) | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
US6312894B1 (en) * | 1995-04-03 | 2001-11-06 | Epoch Pharmaceuticals, Inc. | Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders |
DE69610304T2 (de) * | 1995-05-05 | 2001-04-05 | Perkin-Elmer Corp., Foster City | Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay |
US6008373A (en) | 1995-06-07 | 1999-12-28 | Carnegie Mellon University | Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer |
JP2002515738A (ja) * | 1996-01-23 | 2002-05-28 | アフィメトリックス,インコーポレイティド | 核酸分析法 |
EP0892808B1 (en) * | 1996-04-12 | 2008-05-14 | PHRI Properties, Inc. | Detection probes, kits and assays |
US5800996A (en) * | 1996-05-03 | 1998-09-01 | The Perkin Elmer Corporation | Energy transfer dyes with enchanced fluorescence |
ATE428801T1 (de) | 1996-06-04 | 2009-05-15 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
US5866336A (en) | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
US5696157A (en) | 1996-11-15 | 1997-12-09 | Molecular Probes, Inc. | Sulfonated derivatives of 7-aminocoumarin |
US6893868B2 (en) * | 1997-02-20 | 2005-05-17 | Onco Immunin, Inc. | Homo-doubly labeled compositions for the detection of enzyme activity in biological samples |
US6037137A (en) | 1997-02-20 | 2000-03-14 | Oncoimmunin, Inc. | Fluorogenic peptides for the detection of protease activity |
US5846726A (en) * | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
US6130101A (en) | 1997-09-23 | 2000-10-10 | Molecular Probes, Inc. | Sulfonated xanthene derivatives |
US6485901B1 (en) * | 1997-10-27 | 2002-11-26 | Boston Probes, Inc. | Methods, kits and compositions pertaining to linear beacons |
US6133445A (en) | 1997-12-17 | 2000-10-17 | Carnegie Mellon University | Rigidized trimethine cyanine dyes |
ATE414169T1 (de) | 1998-03-05 | 2008-11-15 | Johnson & Johnson Res Pty Ltd | Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits |
GB2341189B (en) | 1998-08-31 | 2001-06-13 | Amersham Pharm Biotech Inc | Energy transfer dyes |
US6573047B1 (en) | 1999-04-13 | 2003-06-03 | Dna Sciences, Inc. | Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation |
US6472153B1 (en) | 1999-10-26 | 2002-10-29 | Epoch Biosciences, Inc. | Hybridization-triggered fluorescent detection of nucleic acids |
GB2359809B (en) | 1999-12-24 | 2004-01-14 | Sumitomo Chemical Co | Phenylacetylene compound liquid crystal composition, polymer optically anisotropic product, and liquid crystal or optical element |
CA2417986C (en) * | 2000-08-11 | 2013-11-26 | University Of Utah Research Foundation | Single-labeled oligonucleotide probes |
EP1322710B2 (en) | 2000-09-29 | 2015-02-18 | Life Technologies Corporation | Modified carbocyanine dyes and their conjugates |
-
2004
- 2004-11-19 JP JP2006541541A patent/JP2007534308A/ja not_active Withdrawn
- 2004-11-19 WO PCT/US2004/038946 patent/WO2005051967A2/en active Application Filing
- 2004-11-19 US US10/993,625 patent/US7667024B2/en not_active Expired - Fee Related
- 2004-11-19 CN CN200480040647.1A patent/CN101076537B/zh not_active Expired - Fee Related
- 2004-11-19 EP EP04811635A patent/EP1689764B1/en not_active Not-in-force
-
2009
- 2009-12-23 US US12/646,514 patent/US20100240103A1/en not_active Abandoned
-
2011
- 2011-08-15 JP JP2011177659A patent/JP5307206B2/ja not_active Expired - Fee Related
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP2007534308A5 (ja) | ||
Kolpashchikov | Binary malachite green aptamer for fluorescent detection of nucleic acids | |
JP2007506790A5 (ja) | ||
Qaddare et al. | Amplified fluorescent sensing of DNA using luminescent carbon dots and AuNPs/GO as a sensing platform: A novel coupling of FRET and DNA hybridization for homogeneous HIV-1 gene detection at femtomolar level | |
Algar et al. | The application of quantum dots, gold nanoparticles and molecular switches to optical nucleic-acid diagnostics | |
JP2009521707A5 (ja) | ||
Guo et al. | Conformational details of quantum dot-DNA resolved by forster resonance energy transfer lifetime nanoruler | |
JP2004033215A5 (ja) | ||
WO2017008177A1 (en) | Compositions and methods for detection of genetic deafness gene mutation | |
JP2007505908A5 (ja) | ||
JP2009528027A5 (ja) | ||
JP2010539940A5 (ja) | ||
JP2008532485A5 (ja) | ||
ATE487136T1 (de) | Nanopartikel mit gebundenen oligonukleotiden und verwendungen derselben | |
JP2014501497A (ja) | マイクロアレイに基づくアッセイのための粒子と結合させた発光団で標識された分子 | |
JP2008504842A5 (ja) | ||
JP2004194669A5 (ja) | ||
JP2004043819A5 (ja) | ||
JP2007516843A5 (ja) | ||
JP2009542225A5 (ja) | ||
WO2008001376A3 (en) | Detection of analytes in a medium | |
JP2004298200A5 (ja) | ||
JP2005530695A5 (ja) | ||
Chen et al. | FRET with MoS2 nanosheets integrated CRISPR/Cas12a sensors for robust and visual food-borne parasites detection | |
JP2007051233A5 (ja) |